{"database": "metadata", "table": "run_metadata", "rows": [[48057, "SRR7008009", "SRX3940578", "SRS3171380", "SRP140462", "PRJNA450341", "Primary cilia regulate hematopoietic stem and progenitor cell specification through Notch signaling", "PRJNA450341", "Other", "Hematopoietic stem and progenitor cells HSPCs are capable of producing all mature blood lineages  as well as maintaining the self renewal ability throughout life. The hairy like organelle  cilia  are present in most types of vertebrate cells  and play important roles in various biological processes. However  it is unclear whether and how cilia regulate HSPC development in vertebrates. Here  we show that cilia specific genes  involved in primary cilia formation and signaling transduction  are required for HSPC development  especially at the hemogenic endothelium HE specification step in zebrafish embryos. Blocking primary cilia formation or function by genetic or chemical manipulations impaired HSPC development. Mechanistically  we uncover that primary cilia on endothelial cells ECs transduce Notch signal to the earliest HE for proper HSPC specification during embryogenesis. Altogether  our findings reveal a pivotal role of endothelial primary cilia in HSPC development  and may shed lights into in vitro directed differentiation of HSPCs.", null, null, "WT26h rep2", "WT26h 2", "Sample2", null, "strain:zebrafish embryos|isolate:not applicable|dev stage:26 hpf|sex:not applicable|tissue:trunk region|collection date:2017 08 11|geo loc name:China: Beijing|isolation source:not applicable|sample type:normal embryos 2|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA seq of normal embryos2", "WT26h 2", "WT26h 2", "RNA was  isolated from trunk regions of control embryos and fsd1 morphants  for cDNA library. The cDNA library for sequenced using BGISEQ 500 platform.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PCR", "SINGLE", "BGISEQ", "BGISEQ-500", null, "SRP140462", null, null, "WT26h_2_1.fq.gz", "fastq", 1199179850.0, 23983597.0, "WT26h 2 1.fq.gz", "0:50", "A:316605984;C:281184572;G:292581373;T:308419476;N:388445", 50, null, null, null, 316605984, 281184572, 292581373, 308419476, 388445, "SRX3940578", "SRS3171380", "SRA690964", "Institute of Zoology, CHINESE ACADEMY OF SCIENCES|State Key Laboratory of Membrane Biology", "Institute of Zoology, CHINESE ACADEMY OF SCIENCES", 1, 0.95377, null, 0.08617, null, 0.70508, null, 0.4775, null, 50, null, "B", null, "usable mapping rate", "bgi", "bgi", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2018-04-16", "Pharyngula", "Embryo", "Trunk", "Surface Structure"]], "columns": ["rowid", "run.accession", "experiment.accession", "sample.accession", "study.accession", "bioproject", "study.title", "study.alias", "study.type", "study.abstract", "study.attributes", "study.PMIDs", "sample.description", "sample.title", "sample.alias", "sample.centername", "sample.attributes", "GEOsample.title", "GEOsample.dataprocessing", "GEOsample.source", "GEOsample.treatmentprotocol", "GEOsample.extractprotocol", "GEOsample.growthprotocol", "GEOsample.characteristics", "GEOsample.accession", "experiment.title", "experiment.alias", "experiment.library_name", "experiment.design_description", "experiment.library_construction_protocol", "experiment.attributes", "experiment.library_strategy", "experiment.library_source", "experiment.library_selection", "experiment.library_layout", "experiment.platform", "experiment.instrument_model", "experiment.spot_descriptor", "experiment.study_ref", "run.title", "run.attributes", "run.filename", "run.semantic_name", "run.total_bases", "run.total_spots", "run.alias", "run.read_lengths", "run.base_counts", "run.r1_length", "run.r2_length", "run.r3_length", "run.r4_length", "run.Acount", "run.Ccount", "run.Gcount", "run.Tcount", "run.Ncount", "run.experiment", "run.pool_member", "submission.accession", "submission.srasource", "submission.bioprojectsource", "seqdetective.n_mates", "seqdetective.mapping_rate.mate1", "seqdetective.mapping_rate.mate2", "seqdetective.nofeature_rate.mate1", "seqdetective.nofeature_rate.mate2", "seqdetective.sparsity.mate1", "seqdetective.sparsity.mate2", "seqdetective.pos_strand_rate.mate1", "seqdetective.pos_strand_rate.mate2", "seqdetective.readlen.mate1", "seqdetective.readlen.mate2", "seqdetective.judgement.mate1", "seqdetective.judgement.mate2", "seqdetective.judgement.reason", "platform_family", "instrument_generation", "read_bias", "selection_class", "prep_kit", "sc_or_bulk", "tech_class", "technology", "tech_variant", "submission.bioprojectsource.country", "earliest_date", "devstage_curation", "devstage_curation_coarse", "tissue_curation", "tissue_curation_coarse"], "primary_keys": ["rowid"], "primary_key_values": ["48057"], "units": {}, "query_ms": 12.580534999869997}