{"database": "metadata", "table": "run_metadata", "rows": [[43661, "SRR6003529", "SRX3158822", "SRS2490159", "SRP116760", "PRJNA401346", "Raw sequence reads of miR 462/miR 731 knockdwon zebrafish embryos", "PRJNA401346", "Other", "To elucidate the biological roles of miR 462 731 during embryonic development  two morpholinos MOs targeting the mature sequence of miR 462 and miR 731  respectively  were microinjected into zebrafish embryos. A total of 100 embryos of uninjected control  injected with miR 462 MO or injected with miR 731 MO were collected at xxx hpf  respectively  for RNA Seq.", null, null, "100 embyos of uninjected control", "Danio rerio embryos", "Control 48hpf", null, "strain:AB|dev stage:48hpf|sex:not applicable|tissue:embryos|biomaterial provider:Key Laboratory of Freshwater Animal Breeding  College of Fishery  Huazhong Agricultural University|collected by:Chun Xiao Huang|sample type:whole embryo|treatment:uninjected control|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of zebrafish: 48hpf embryos", "Control 48hpf", "Control 48hpf", "Sequencing libraries were generated using NEBNext? Ultra? RNA LibraryPrep Kit for Illumina? NEB  USA following manufacturer\u00a1\u00afs recommendations and indexcodes were added to attribute sequences to each sample.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RT-PCR", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP116760", null, null, null, null, 3723608000.0, 14894432.0, "Contr48 2.fq.gz", "0:125 1:125", "A:947710287;C:910496767;G:932389849;T:932231152;N:779945", 125, 125, null, null, 947710287, 910496767, 932389849, 932231152, 779945, "SRX3158822", "SRS2490159", "SRA605241", "Huazhong Agricultural University|College of Fisheries", "Huazhong Agricultural University", 2, 0.94491, 0.94085, 0.0282, 0.02794, 0.70928, 0.71636, 0.46153, 0.4685, 125, 125, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "other", "nebnext", "bulk", "unknown", "unknown", null, "China", "2017-09-05", "Hatching", "Embryo", "Embryo Imprecise", "All anatomical structures"]], "columns": ["rowid", "run.accession", "experiment.accession", "sample.accession", "study.accession", "bioproject", "study.title", "study.alias", "study.type", "study.abstract", "study.attributes", "study.PMIDs", "sample.description", "sample.title", "sample.alias", "sample.centername", "sample.attributes", "GEOsample.title", "GEOsample.dataprocessing", "GEOsample.source", "GEOsample.treatmentprotocol", "GEOsample.extractprotocol", "GEOsample.growthprotocol", "GEOsample.characteristics", "GEOsample.accession", "experiment.title", "experiment.alias", "experiment.library_name", "experiment.design_description", "experiment.library_construction_protocol", "experiment.attributes", "experiment.library_strategy", "experiment.library_source", "experiment.library_selection", "experiment.library_layout", "experiment.platform", "experiment.instrument_model", "experiment.spot_descriptor", "experiment.study_ref", "run.title", "run.attributes", "run.filename", "run.semantic_name", "run.total_bases", "run.total_spots", "run.alias", "run.read_lengths", "run.base_counts", "run.r1_length", "run.r2_length", "run.r3_length", "run.r4_length", "run.Acount", "run.Ccount", "run.Gcount", "run.Tcount", "run.Ncount", "run.experiment", "run.pool_member", "submission.accession", "submission.srasource", "submission.bioprojectsource", "seqdetective.n_mates", "seqdetective.mapping_rate.mate1", "seqdetective.mapping_rate.mate2", "seqdetective.nofeature_rate.mate1", "seqdetective.nofeature_rate.mate2", "seqdetective.sparsity.mate1", "seqdetective.sparsity.mate2", "seqdetective.pos_strand_rate.mate1", "seqdetective.pos_strand_rate.mate2", "seqdetective.readlen.mate1", "seqdetective.readlen.mate2", "seqdetective.judgement.mate1", "seqdetective.judgement.mate2", "seqdetective.judgement.reason", "platform_family", "instrument_generation", "read_bias", "selection_class", "prep_kit", "sc_or_bulk", "tech_class", "technology", "tech_variant", "submission.bioprojectsource.country", "earliest_date", "devstage_curation", "devstage_curation_coarse", "tissue_curation", "tissue_curation_coarse"], "primary_keys": ["rowid"], "primary_key_values": ["43661"], "units": {}, "query_ms": 13.285271001223009}