{"database": "metadata", "table": "run_metadata", "rows": [[42227, "SRR5837498", "SRX3013992", "SRS2363586", "SRP112722", "PRJNA385064", "Transcriptomic effects of 17 alpha methyltestosterone on zebrafish brain sexual development.", "PRJNA385064", "Other", "Sexual differentiation in zebrafish is complex. Although zebrafish sex determination is primarily genetic  hormonal and environmental factors can influence sexual development. Androgen treatment triggers phenotypic and behavioural masculinisation in zebrafish. 17 alpha methyltestosterone MT  a synthetic androgen  is routinely used in aquaculture for production of all male populations. However  the molecular mechanisms underlying 17 alpha methyltestosterone induced brain and behavioural masculinisation in fish are poorly understood. In this study  we analysed brain transcriptomes of zebrafish treated with 17 alpha methyltestosterone during gonadal development from 20 dpf to 40 dpf and 60 dpf and compared them with brain transcriptomes of untreated female and male zebrafish. These data give insight into the role of androgens in teleost brain sexual differentiation.", null, null, null, null, "40MTB1", null, "strain:Tgvas:egfp|age:40 dpf|dev stage:juvenile|sex:male|tissue:brain|genotype:Tgvas:egfp|sample type:whole brain|treatment:methyltestoster1 treated|replicate:40 dpf MT treated male brain Replicate 1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of danio rerio: 40 dpf methyltestoster1 treated male brain", "40MTB1", "40MTB1", "1 \u00b5g of total RNA was used with the Illumina TruSeq RNA sample preparation kit v2 for construction of each library. 100 bp paired end reads were generated using the HiSeq 2000 platform.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina HiSeq 2000", null, "SRP112722", null, null, "BC1JJDACXX_NZGL00075_40MTMB1_GATCAG_L005_R1_001.fastq.gz BC1JJDACXX_NZGL00075_40MTMB1_GATCAG_L005_R2_001.fastq.gz C1PF2ACXX_NZGL00075_40MTMB1_GATCAG_L003_R1_001.fastq.gz C1PF2ACXX_NZGL00075_40MTMB1_GATCAG_L003_R2_001.fastq.gz C1PF2ACXX_NZGL00075_40MTMB1_GATCAG_L004_R1_001.fastq.gz C1PF2ACXX_NZGL00075_40MTMB1_GATCAG_L004_R2_001.fastq.gz", "fastq fastq fastq fastq fastq fastq", 3626521400.0, 18132607.0, "C1PF2ACXX NZGL00075 40MTMB1 GATCAG L004 R2 001.fastq.gz", "0:100 1:100", "A:1008131839;C:809568850;G:796148693;T:1004689114;N:7982904", 100, 100, null, null, 1008131839, 809568850, 796148693, 1004689114, 7982904, "SRX3013992", "SRS2363586", "SRA588281", "University of Otago|Anatomy", "University of Otago", 2, 0.94168, 0.94206, 0.12488, 0.12478, 0.69656, 0.69514, 0.51439, 0.51446, 100, 100, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "cdna_unspecified", "trueseq", "bulk", "unknown", "unknown", null, "New Zealand", "2018-07-31", "Juvenile", "Juvenile", "Brain", "Nervous System"]], "columns": ["rowid", "run.accession", "experiment.accession", "sample.accession", "study.accession", "bioproject", "study.title", "study.alias", "study.type", "study.abstract", "study.attributes", "study.PMIDs", "sample.description", "sample.title", "sample.alias", "sample.centername", "sample.attributes", "GEOsample.title", "GEOsample.dataprocessing", "GEOsample.source", "GEOsample.treatmentprotocol", "GEOsample.extractprotocol", "GEOsample.growthprotocol", "GEOsample.characteristics", "GEOsample.accession", "experiment.title", "experiment.alias", "experiment.library_name", "experiment.design_description", "experiment.library_construction_protocol", "experiment.attributes", "experiment.library_strategy", "experiment.library_source", "experiment.library_selection", "experiment.library_layout", "experiment.platform", "experiment.instrument_model", "experiment.spot_descriptor", "experiment.study_ref", "run.title", "run.attributes", "run.filename", "run.semantic_name", "run.total_bases", "run.total_spots", "run.alias", "run.read_lengths", "run.base_counts", "run.r1_length", "run.r2_length", "run.r3_length", "run.r4_length", "run.Acount", "run.Ccount", "run.Gcount", "run.Tcount", "run.Ncount", "run.experiment", "run.pool_member", "submission.accession", "submission.srasource", "submission.bioprojectsource", "seqdetective.n_mates", "seqdetective.mapping_rate.mate1", "seqdetective.mapping_rate.mate2", "seqdetective.nofeature_rate.mate1", "seqdetective.nofeature_rate.mate2", "seqdetective.sparsity.mate1", "seqdetective.sparsity.mate2", "seqdetective.pos_strand_rate.mate1", "seqdetective.pos_strand_rate.mate2", "seqdetective.readlen.mate1", "seqdetective.readlen.mate2", "seqdetective.judgement.mate1", "seqdetective.judgement.mate2", "seqdetective.judgement.reason", "platform_family", "instrument_generation", "read_bias", "selection_class", "prep_kit", "sc_or_bulk", "tech_class", "technology", "tech_variant", "submission.bioprojectsource.country", "earliest_date", "devstage_curation", "devstage_curation_coarse", "tissue_curation", "tissue_curation_coarse"], "primary_keys": ["rowid"], "primary_key_values": ["42227"], "units": {}, "query_ms": 10.724125000706408}