{"database": "metadata", "table": "run_metadata", "rows": [[41986, "SRR5378553", "SRX2673815", "SRS2073010", "SRP102493", "PRJNA379493", "Transcriptomic effects of 17 alpha methyltestosterone in gonads during zebrafish gonad development.", "PRJNA379493", "Other", "Sexual differentiation in zebrafish is complex. Although zebrafish sex determination is primarily genetic  hormonal and environmental factors can influence sexual development. 17 alpha methyltestosterone MT  a synthetic androgen  induces female to male sex reversal in zebrafish. MT treatment is routinely used in aquaculture for production of all male populations. However  the molecular mechanisms underlying 17 alpha methyltestosterone induced gonad masculinisation in fish are poorly understood.In this study  we analysed gonad transcriptomes of zebrafish treated with 17 alpha methyltestosterone during gonadal development from 20 dpf to 40 dpf and 60 dpf and compared them with testis and ovary transcriptomes of untreated zebrafish. These data improve our understanding of the role of androgens in teleost sex differentiation.", null, "pubmed:28738802", null, null, "60MT2", null, "strain:Tgvas:egfp|age:60 dpf|dev stage:juvenile|sex:male|tissue:testis|genotype:Tgvas:egfp|sample type:gonadal tissue|treatment:methyltestoster1 treated|replicate:60 dpf MT treated testis Replicate 2|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of danio rerio: 60 dpf methyltestoster1 treated testis", "60MT2", "60MT2", "1 \u00b5g of total RNA was used with the Illumina TruSeq RNA sample preparation kit v2 for construction of each library. 100 bp paired end reads were generated using the HiSeq 2000 platform.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina HiSeq 2000", "<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>200</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>101</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>", "SRP102493", null, null, "C1PF2ACXX_NZGL00075_60MTMT2_AGTTCC_L005_R1_001.fastq.gz C1PF2ACXX_NZGL00075_60MTMT2_AGTTCC_L005_R2_001.fastq.gz", "fastq fastq", 3535698400.0, 17678492.0, "C1PF2ACXX NZGL00075 60MTMT2 AGTTCC L005 R1 001.fastq.gz", "0:100 1:100", "A:953667018;C:819969570;G:806855071;T:952124680;N:3082061", 100, 100, null, null, 953667018, 819969570, 806855071, 952124680, 3082061, "SRX2673815", "SRS2073010", "SRA549173", "University of Otago|Anatomy", "University of Otago", 2, 0.94013, 0.93993, 0.09566, 0.09661, 0.62591, 0.62731, 0.48997, 0.488, 100, 100, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "cdna_unspecified", "trueseq", "bulk", "unknown", "unknown", null, "New Zealand", "2017-09-26", "Juvenile", "Juvenile", "Gonad", "Reproductive System"]], "columns": ["rowid", "run.accession", "experiment.accession", "sample.accession", "study.accession", "bioproject", "study.title", "study.alias", "study.type", "study.abstract", "study.attributes", "study.PMIDs", "sample.description", "sample.title", "sample.alias", "sample.centername", "sample.attributes", "GEOsample.title", "GEOsample.dataprocessing", "GEOsample.source", "GEOsample.treatmentprotocol", "GEOsample.extractprotocol", "GEOsample.growthprotocol", "GEOsample.characteristics", "GEOsample.accession", "experiment.title", "experiment.alias", "experiment.library_name", "experiment.design_description", "experiment.library_construction_protocol", "experiment.attributes", "experiment.library_strategy", "experiment.library_source", "experiment.library_selection", "experiment.library_layout", "experiment.platform", "experiment.instrument_model", "experiment.spot_descriptor", "experiment.study_ref", "run.title", "run.attributes", "run.filename", "run.semantic_name", "run.total_bases", "run.total_spots", "run.alias", "run.read_lengths", "run.base_counts", "run.r1_length", "run.r2_length", "run.r3_length", "run.r4_length", "run.Acount", "run.Ccount", "run.Gcount", "run.Tcount", "run.Ncount", "run.experiment", "run.pool_member", "submission.accession", "submission.srasource", "submission.bioprojectsource", "seqdetective.n_mates", "seqdetective.mapping_rate.mate1", "seqdetective.mapping_rate.mate2", "seqdetective.nofeature_rate.mate1", "seqdetective.nofeature_rate.mate2", "seqdetective.sparsity.mate1", "seqdetective.sparsity.mate2", "seqdetective.pos_strand_rate.mate1", "seqdetective.pos_strand_rate.mate2", "seqdetective.readlen.mate1", "seqdetective.readlen.mate2", "seqdetective.judgement.mate1", "seqdetective.judgement.mate2", "seqdetective.judgement.reason", "platform_family", "instrument_generation", "read_bias", "selection_class", "prep_kit", "sc_or_bulk", "tech_class", "technology", "tech_variant", "submission.bioprojectsource.country", "earliest_date", "devstage_curation", "devstage_curation_coarse", "tissue_curation", "tissue_curation_coarse"], "primary_keys": ["rowid"], "primary_key_values": ["41986"], "units": {}, "query_ms": 9.98367799911648}