{"database": "metadata", "table": "run_metadata", "rows": [[41976, "SRR5378563", "SRX2673825", "SRS2073020", "SRP102493", "PRJNA379493", "Transcriptomic effects of 17 alpha methyltestosterone in gonads during zebrafish gonad development.", "PRJNA379493", "Other", "Sexual differentiation in zebrafish is complex. Although zebrafish sex determination is primarily genetic  hormonal and environmental factors can influence sexual development. 17 alpha methyltestosterone MT  a synthetic androgen  induces female to male sex reversal in zebrafish. MT treatment is routinely used in aquaculture for production of all male populations. However  the molecular mechanisms underlying 17 alpha methyltestosterone induced gonad masculinisation in fish are poorly understood.In this study  we analysed gonad transcriptomes of zebrafish treated with 17 alpha methyltestosterone during gonadal development from 20 dpf to 40 dpf and 60 dpf and compared them with testis and ovary transcriptomes of untreated zebrafish. These data improve our understanding of the role of androgens in teleost sex differentiation.", null, "pubmed:28738802", null, null, "40CO1", null, "strain:Tgvas:egfp|age:40 dpf|dev stage:juvenile|sex:female|tissue:ovary|genotype:Tgvas:egfp|sample type:gonadal tissue|treatment:untreated|replicate:40 dpf control ovary Replicate 1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of danio rerio: 40 dpf untreated ovary", "40CO1", "40CO1", "1 \u00b5g of total RNA was used with the Illumina TruSeq RNA sample preparation kit v2 for construction of each library. 100 bp paired end reads were generated using the HiSeq 2000 platform.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina HiSeq 2000", null, "SRP102493", null, null, "C1PF2ACXX_NZGL00075_40SCFO1_CGATGT_L005_R1_001.fastq.gz C1PF2ACXX_NZGL00075_40SCFO1_CGATGT_L005_R2_001.fastq.gz", "fastq fastq", 2794786000.0, 13973930.0, "C1PF2ACXX NZGL00075 40SCFO1 CGATGT L005 R2 001.fastq.gz", "0:100 1:100", "A:733793512;C:667693170;G:653892765;T:737072945;N:2333608", 100, 100, null, null, 733793512, 667693170, 653892765, 737072945, 2333608, "SRX2673825", "SRS2073020", "SRA549173", "University of Otago|Anatomy", "University of Otago", 2, 0.93457, 0.93414, 0.03501, 0.03513, 0.74416, 0.74306, 0.45464, 0.46296, 100, 100, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "cdna_unspecified", "trueseq", "bulk", "unknown", "unknown", null, "New Zealand", "2017-09-26", "Juvenile", "Juvenile", "Gonad", "Reproductive System"]], "columns": ["rowid", "run.accession", "experiment.accession", "sample.accession", "study.accession", "bioproject", "study.title", "study.alias", "study.type", "study.abstract", "study.attributes", "study.PMIDs", "sample.description", "sample.title", "sample.alias", "sample.centername", "sample.attributes", "GEOsample.title", "GEOsample.dataprocessing", "GEOsample.source", "GEOsample.treatmentprotocol", "GEOsample.extractprotocol", "GEOsample.growthprotocol", "GEOsample.characteristics", "GEOsample.accession", "experiment.title", "experiment.alias", "experiment.library_name", "experiment.design_description", "experiment.library_construction_protocol", "experiment.attributes", "experiment.library_strategy", "experiment.library_source", "experiment.library_selection", "experiment.library_layout", "experiment.platform", "experiment.instrument_model", "experiment.spot_descriptor", "experiment.study_ref", "run.title", "run.attributes", "run.filename", "run.semantic_name", "run.total_bases", "run.total_spots", "run.alias", "run.read_lengths", "run.base_counts", "run.r1_length", "run.r2_length", "run.r3_length", "run.r4_length", "run.Acount", "run.Ccount", "run.Gcount", "run.Tcount", "run.Ncount", "run.experiment", "run.pool_member", "submission.accession", "submission.srasource", "submission.bioprojectsource", "seqdetective.n_mates", "seqdetective.mapping_rate.mate1", "seqdetective.mapping_rate.mate2", "seqdetective.nofeature_rate.mate1", "seqdetective.nofeature_rate.mate2", "seqdetective.sparsity.mate1", "seqdetective.sparsity.mate2", "seqdetective.pos_strand_rate.mate1", "seqdetective.pos_strand_rate.mate2", "seqdetective.readlen.mate1", "seqdetective.readlen.mate2", "seqdetective.judgement.mate1", "seqdetective.judgement.mate2", "seqdetective.judgement.reason", "platform_family", "instrument_generation", "read_bias", "selection_class", "prep_kit", "sc_or_bulk", "tech_class", "technology", "tech_variant", "submission.bioprojectsource.country", "earliest_date", "devstage_curation", "devstage_curation_coarse", "tissue_curation", "tissue_curation_coarse"], "primary_keys": ["rowid"], "primary_key_values": ["41976"], "units": {}, "query_ms": 7.9861769918352365}