{"database": "metadata", "table": "run_metadata", "rows": [[41678, "SRR5119937", "SRX2435212", "SRS1870208", "SRP095327", "PRJNA358007", "Estrogen sensing by GPER1 activates PI3K/mTOR to promote gender dimorphism in liver growth and cancer", "GSE92544", "Transcriptome Analysis", "Liver cancer is a common cause of cancer death  with a male predominant incidence due  in part  to increased estrogen levels in cirrhotic patients. It is unknown  however  how estrogen is sensed to influence this process. Here  we show that estrogen activates the G protein coupled estrogen receptor 1 GPER1  expressed in hepatocytes  to enhancing hepatocyte size  cell cycle progression and cell proliferation  thereby increasing liver growth in zebrafish larvae and adults. GPER1 stimulation activates PI3K/mTOR signaling  and mTOR is essential for both normal and regenerative organ growth. Genetic loss of GPER1 diminishes and estrogen exposure accelerates chemical carcinogenesis  specifically in males. Chemical inhibition of GPER1 significantly reduces cancer incidence and progression in a gender specific fashion. Our studies identify GPER1 as a hepatic estrogen sensor that mediates gender dimorphic growth  mTOR activation  and can serve as a therapeutic target for liver cancer treatment. Overall design: RNA Seq analysis of control DMSO treated livers and \u00df Estradiol exposed livers were examined.", null, "pubmed:30641053", null, "GC9 WT Male 3", "GSM2432108", null, "source name:liver  DMSO|tissue:liver|developmental stage:adult|genotype:Wild type", "GC9 WT Male 3", "Sequenced reads were trimmed for adaptor sequence  and masked for low complexity or low quality sequence using cutadapt  then mapped to UCSC danRer7 whole genome using Tophat 2.0.11 without xxx splicing form calls Transcript abundance and differential expression were calculated with Cufflinks 2.2.1. FPKM values were used to normalize and quantify each transcripts Genome build: danRer7 Supplementary files format and content: excel files include RPKM values for each Sample", "liver  DMSO", "Zebrafish were raised until at 3 mpf Fish were then treated daily with DMSO or \u03b2 Estradiol for a period of 6 weeks.", "Adult zebrafish livers were surgically isolated and RNA was extracted in Trizol Life Technologies  isopropanol precipitated and ethanol washed. RNA was DNase treated using TURBO DNA free kit Life Technologies and RNA quality and quantity were determined using Qubit Thermo Fisher and bioanalyzer Agilent. For library construction  NEBNext Directional RNA Library Prep Kit for Illumina #E7420L was used according to the company's protocol", "All zebrafish was maintained according to the standard Institutional Animal Care and Use Committee guidelines IACUC. At 3 mpf  a selected group of wild type male and female zebrafish were seperated into individual tanks to control food consumption.", "tissue:liver|developmental stage:adult|genotype:Wild type", "GSM2432108", "GSM2432108: GC9 WT Male 3; Danio rerio; RNA Seq", "GSM2432108", null, "1", "Adult zebrafish livers were surgically isolated and RNA was extracted in Trizol Life Technologies  isopropanol precipitated and ethanol washed. RNA was DNase treated using TURBO DNA free kit Life Technologies and RNA quality and quantity were determined using Qubit Thermo Fisher and bioanalyzer Agilent. For library construction  NEBNext Directional RNA Library Prep Kit for Illumina #E7420L was used according to the company's protocol", "GEO Accession:GSM2432108", "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "SRP095327", null, null, "GC9_WT_Male_3_R1_.final.fastq.gz", "fastq", 1512010654.0, 20022544.0, "GSM2432108 r1", "0:75.52 1:0", "A:384125071;C:357935858;G:341294088;T:428626442;N:29195", 75, 0, null, null, 384125071, 357935858, 341294088, 428626442, 29195, "SRX2435212", "SRS1870208", "SRA505252", "GEO", "Oncology/Hematology, Boston Children's Hospital", 1, 0.95739, null, 0.06355, null, 0.82146, null, 0.54213, null, 76, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "random_priming", "nebnext", "bulk", "unknown", "unknown", null, "United States", "2016-12-19", "Adult", "Adult", "Liver", "Liver and Biliary System"]], "columns": ["rowid", "run.accession", "experiment.accession", "sample.accession", "study.accession", "bioproject", "study.title", "study.alias", "study.type", "study.abstract", "study.attributes", "study.PMIDs", "sample.description", "sample.title", "sample.alias", "sample.centername", "sample.attributes", "GEOsample.title", "GEOsample.dataprocessing", "GEOsample.source", "GEOsample.treatmentprotocol", "GEOsample.extractprotocol", "GEOsample.growthprotocol", "GEOsample.characteristics", "GEOsample.accession", "experiment.title", "experiment.alias", "experiment.library_name", "experiment.design_description", "experiment.library_construction_protocol", "experiment.attributes", "experiment.library_strategy", "experiment.library_source", "experiment.library_selection", "experiment.library_layout", "experiment.platform", "experiment.instrument_model", "experiment.spot_descriptor", "experiment.study_ref", "run.title", "run.attributes", "run.filename", "run.semantic_name", "run.total_bases", "run.total_spots", "run.alias", "run.read_lengths", "run.base_counts", "run.r1_length", "run.r2_length", "run.r3_length", "run.r4_length", "run.Acount", "run.Ccount", "run.Gcount", "run.Tcount", "run.Ncount", "run.experiment", "run.pool_member", "submission.accession", "submission.srasource", "submission.bioprojectsource", "seqdetective.n_mates", "seqdetective.mapping_rate.mate1", "seqdetective.mapping_rate.mate2", "seqdetective.nofeature_rate.mate1", "seqdetective.nofeature_rate.mate2", "seqdetective.sparsity.mate1", "seqdetective.sparsity.mate2", "seqdetective.pos_strand_rate.mate1", "seqdetective.pos_strand_rate.mate2", "seqdetective.readlen.mate1", "seqdetective.readlen.mate2", "seqdetective.judgement.mate1", "seqdetective.judgement.mate2", "seqdetective.judgement.reason", "platform_family", "instrument_generation", "read_bias", "selection_class", "prep_kit", "sc_or_bulk", "tech_class", "technology", "tech_variant", "submission.bioprojectsource.country", "earliest_date", "devstage_curation", "devstage_curation_coarse", "tissue_curation", "tissue_curation_coarse"], "primary_keys": ["rowid"], "primary_key_values": ["41678"], "units": {}, "query_ms": 5.498074999195524}