{"database": "metadata", "table": "run_metadata", "rows": [[39630, "SRR1947880", "SRX981061", "SRS895863", "SRP056748", "PRJNA279991", "Zebrafish Mespaa regulates miR 430 expression during gastrulation and  initiates cardiac laterality", "PRJNA279991", "Other", "Transcriptional events during initial vertebrate heart development in vivo remain poorly understood. Mesp1  a bHLH transcription factor  has been described as the earliest transcriptional regulator of cardiac progenitors in multiple species  and represents an excellent candidate for the investigation of relevant targets during cardiovascular development. We report here that both depletion and mutation of Mespaa  the zebrafish homolog of mammalian Mesp1  lead to randomization of cardiac looping  together with significant cardiac morphogenesis defects. These disruptions are preceded by a defect in cardiac left right asymmetry. Surprisingly  the defect in asymmetry was found to occur independently of defects in the transient organ of laterality  the Kupffer\u2019s vesicle KV. We show that Mespaa regulates miR 430 expression during gastrulation to control the levels of Nodal signaling  and that this regulation is required for asymmetric laterality signaling in the prospective heart field. Ectopic expression of miR 430 is sufficient to induce cardiac laterality defects  and consistent with Mespaa over expression in this system  the reduction of miR 430 leads to cardia bifida. This study reveals a novel transcriptional regulation of miR 430 by Mespaa and a role for this pathway in cardiac laterality during gastrulation.", null, null, null, "Danio rerio injected with negative control morpholino  5.5hpf", "Zebrafish injected with untargeted negative control morpholino", null, "strain:Ekkwill|dev stage:5.5 hpf|sex:pooled male and female|tissue:whole embryo|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Zebrafish Mespaa regulates miR 430 expression during gastrulation and  initiates cardiac laterality", "Zebrafish injected with untargeted negative control morpholino replicate 2", "Zebrafish injected with untargeted negative control morpholino replicate 2", "1", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "Illumina HiSeq 2000", null, "SRP056748", null, "loader:latf load", "Std-230413_GTGTTA_L008_R1.fastq.gz Std-230413_GTGTTA_L008_R2.fastq.gz", "fastq fastq", 10015452496.0, 49581448.0, "RNAseq Danio rerio  5.5hpf  negative control morpholino replicate2", "0:101 1:101", "A:2883382922;C:1998043392;G:2046542450;T:2937071125;N:150412607", 101, 101, null, null, 2883382922, 1998043392, 2046542450, 2937071125, 150412607, "SRX981061", "SRS895863", "SRA249481", "Gladstone Institute|Bioinformatics", "Gladstone Institutes", 2, 0.8766, 0.85002, 0.17158, 0.25762, 0.77167, 0.89057, 0.64584, 0.66342, 101, 101, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "United States", "2016-03-31", "Gastrula", "Embryo", "Whole Organism", "All anatomical structures"]], "columns": ["rowid", "run.accession", "experiment.accession", "sample.accession", "study.accession", "bioproject", "study.title", "study.alias", "study.type", "study.abstract", "study.attributes", "study.PMIDs", "sample.description", "sample.title", "sample.alias", "sample.centername", "sample.attributes", "GEOsample.title", "GEOsample.dataprocessing", "GEOsample.source", "GEOsample.treatmentprotocol", "GEOsample.extractprotocol", "GEOsample.growthprotocol", "GEOsample.characteristics", "GEOsample.accession", "experiment.title", "experiment.alias", "experiment.library_name", "experiment.design_description", "experiment.library_construction_protocol", "experiment.attributes", "experiment.library_strategy", "experiment.library_source", "experiment.library_selection", "experiment.library_layout", "experiment.platform", "experiment.instrument_model", "experiment.spot_descriptor", "experiment.study_ref", "run.title", "run.attributes", "run.filename", "run.semantic_name", "run.total_bases", "run.total_spots", "run.alias", "run.read_lengths", "run.base_counts", "run.r1_length", "run.r2_length", "run.r3_length", "run.r4_length", "run.Acount", "run.Ccount", "run.Gcount", "run.Tcount", "run.Ncount", "run.experiment", "run.pool_member", "submission.accession", "submission.srasource", "submission.bioprojectsource", "seqdetective.n_mates", "seqdetective.mapping_rate.mate1", "seqdetective.mapping_rate.mate2", "seqdetective.nofeature_rate.mate1", "seqdetective.nofeature_rate.mate2", "seqdetective.sparsity.mate1", "seqdetective.sparsity.mate2", "seqdetective.pos_strand_rate.mate1", "seqdetective.pos_strand_rate.mate2", "seqdetective.readlen.mate1", "seqdetective.readlen.mate2", "seqdetective.judgement.mate1", "seqdetective.judgement.mate2", "seqdetective.judgement.reason", "platform_family", "instrument_generation", "read_bias", "selection_class", "prep_kit", "sc_or_bulk", "tech_class", "technology", "tech_variant", "submission.bioprojectsource.country", "earliest_date", "devstage_curation", "devstage_curation_coarse", "tissue_curation", "tissue_curation_coarse"], "primary_keys": ["rowid"], "primary_key_values": ["39630"], "units": {}, "query_ms": 5.555381000021953}