{"database": "metadata", "table": "run_metadata", "rows": [[37961, "SRR1205174", "SRX501301", "SRS582373", "SRP040561", "PRJNA242641", "Gene expression analysis of hair cell regeneration in the zebrafish lateral line", "GSE56176", "Transcriptome Analysis", "Deafness due to the terminal loss of inner ear hair cells is one of the most common sensory diseases. However  non mammalian animals e.g. birds  amphibian and fish regenerate damaged hair cells. In order to better understand the reasons underpinning such regeneration disparities in vertebrates  we set out to define the changes in gene expression associated with the regeneration of hair cells in the zebrafish lateral line at high resolution. We performed RNA Seq analyses on regenerating support cells purified by fluorescence activated cell sorting FACS. The zebrafish lateral line provides an experimentally accessible system to define the complex signaling events triggered by injury and regeneration  because these cells can be acutely killed by exposure to neomycin  post which they regenerate rapidly. Lateral line hair cells are located in the center of a mechanosensory organ known as the neuromast and are surrounded by inner support cells and an outer ring of mantle cells. TgsqET20 larvae express GFP strongly in mantle cells and to a lesser degree in inner support cells. We isolated GFP positive and GFP negative cells from 5 dpf dpf TgsqET20 larvae at 1  3 and 5 hours post neomycin treatment  as well as from a non treated control. Overall design: Transgenic zebrafish TgsqET20 larvae at 5 dpf were exposed to neomycin  dissociated  and FACS sorted into GFP positive and GFP negative populations at xxx  3  and 5 hours following treatment  along with a mock treated 1 hr control. The experiment was performed in triplicate  for a total of 24 samples.", null, "pubmed:24706903", null, "cntl neo 5hr 3", "GSM1357182", null, "tissue:GFP negative cells from 5 dpf larvae|drug:neomycin|time:5hr|gfp status:negative|genotype:TgsqET20|experiment date:Apr2012", "cntl neo 5hr 3", "Data was processed with CASAVA version 1.8.2 Sequence reads in fastq format were mapped to danRer7using tophat 1.4.1 with options \u2013g 1 and a GTF description of transcripts based on Ensembl 63. FPKM values for these transcripts were generated using cufflinks v1.3. Genome build: danRer7 Supplementary files format and content: FPKM values per gene.", "GFP negative cells from 5 dpf larvae", "For neomycin treatment  5dpf larvae were treated for 30min with 300\u03bcM neomycin Fisher BioReagents diluted in 0.5X E2 medium  rinsed three times and recovered in 0.5X E2 medium at 28.5\u00b0C for the indicated time.", "Two hundred 5dpf untreated TgsqET20 control or neomycin treated TgsqET20 larvae were anesthetized  collected in a 2mL tube  dissociated with trypsin and filtered to remove un dissociated tissue. A two gate FACS strategy was used to select only living target cells from excesses of dead cells and cellular debris of the larval cell suspensions see Materials and Methods of associated manuscript for detailed FACS protocol. Approximately 30 000 GFP  or GFP+ cells were used for total RNA extraction using Trizol Invitrogen following the manufacturer\u2019s manual. Libraries for RNA sequencing were made with poly A selected mRNA using the Illumina TruSeq RNA library construction kit v2 Illumina. The resulting libraries were purified using Agencourt AMPure XP system Backman Coulter  then quantified using a Bioanalyzer or Qubit Fluorometer Life Technologies.", "Larvae were generated by paired matings and raised in 0.5X E2 medium at 28.5\u00b0C. GFP positive larvae were sorted from wild type larvae at 48 hpf under fluorescent stereoscope.", "drug:neomycin|time:5hr|gfp status:negative|genotype:TgsqET20|experiment date:Apr2012", "GSM1357182", "GSM1357182: cntl neo 5hr 3; Danio rerio; RNA Seq", "GSM1357182", null, "1", "Two hundred 5dpf untreated TgsqET20 control or neomycin treated TgsqET20 larvae were anesthetized  collected in a 2mL tube  dissociated with trypsin and filtered to remove un dissociated tissue. A two gate FACS strategy was used to select only living target cells from excesses of dead cells and cellular debris of the larval cell suspensions see Materials and Methods of associated manuscript for detailed FACS protocol. Approximately 30 000 GFP  or GFP+ cells were used for total RNA extraction using Trizol Invitrogen following the manufacturer\u2019s manual. Libraries for RNA sequencing were made with poly A selected mRNA using the Illumina TruSeq RNA library construction kit v2 Illumina. The resulting libraries were purified using Agencourt AMPure XP system Backman Coulter  then quantified using a Bioanalyzer or Qubit Fluorometer Life Technologies.", "GEO Accession:GSM1357182", "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "SINGLE", "ILLUMINA", "Illumina HiSeq 2000", null, "SRP040561", null, "loader:latf load", "cntl_neo_5_3.fastq.gz", "fastq", 1630216750.0, 32604335.0, "GSM1357182 r1", "0:50 1:0", "A:435441738;C:384703554;G:377838654;T:432163463;N:69341", 50, 0, null, null, 435441738, 384703554, 377838654, 432163463, 69341, "SRX501301", "SRS582373", "SRA149178", "GEO", "Seidel, Genomics, Stowers Institute", 1, 0.93632, null, 0.20492, null, 0.67551, null, 0.50281, null, 50, null, "B", null, "usable mapping rate", "illumina", "hiseq_era", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "United States", "2014-03-25", "Multi-stage", "Multi-stage", "Undetermined", "Undetermined"]], "columns": ["rowid", "run.accession", "experiment.accession", "sample.accession", "study.accession", "bioproject", "study.title", "study.alias", "study.type", "study.abstract", "study.attributes", "study.PMIDs", "sample.description", "sample.title", "sample.alias", "sample.centername", "sample.attributes", "GEOsample.title", "GEOsample.dataprocessing", "GEOsample.source", "GEOsample.treatmentprotocol", "GEOsample.extractprotocol", "GEOsample.growthprotocol", "GEOsample.characteristics", "GEOsample.accession", "experiment.title", "experiment.alias", "experiment.library_name", "experiment.design_description", "experiment.library_construction_protocol", "experiment.attributes", "experiment.library_strategy", "experiment.library_source", "experiment.library_selection", "experiment.library_layout", "experiment.platform", "experiment.instrument_model", "experiment.spot_descriptor", "experiment.study_ref", "run.title", "run.attributes", "run.filename", "run.semantic_name", "run.total_bases", "run.total_spots", "run.alias", "run.read_lengths", "run.base_counts", "run.r1_length", "run.r2_length", "run.r3_length", "run.r4_length", "run.Acount", "run.Ccount", "run.Gcount", "run.Tcount", "run.Ncount", "run.experiment", "run.pool_member", "submission.accession", "submission.srasource", "submission.bioprojectsource", "seqdetective.n_mates", "seqdetective.mapping_rate.mate1", "seqdetective.mapping_rate.mate2", "seqdetective.nofeature_rate.mate1", "seqdetective.nofeature_rate.mate2", "seqdetective.sparsity.mate1", "seqdetective.sparsity.mate2", "seqdetective.pos_strand_rate.mate1", "seqdetective.pos_strand_rate.mate2", "seqdetective.readlen.mate1", "seqdetective.readlen.mate2", "seqdetective.judgement.mate1", "seqdetective.judgement.mate2", "seqdetective.judgement.reason", "platform_family", "instrument_generation", "read_bias", "selection_class", "prep_kit", "sc_or_bulk", "tech_class", "technology", "tech_variant", "submission.bioprojectsource.country", "earliest_date", "devstage_curation", "devstage_curation_coarse", "tissue_curation", "tissue_curation_coarse"], "primary_keys": ["rowid"], "primary_key_values": ["37961"], "units": {}, "query_ms": 13.387308001256315}