{"database": "metadata", "table": "run_metadata", "rows": [[37121, "SRR952244", "SRX334986", "SRS469877", "SRP028754", "PRJNA214896", "Danio rerio Transcriptome or Gene expression", "PRJNA214896", "Other", "During early vertebrate development  various small non coding RNAs sRNAs such as MicroRNAs miRNAs and Piwi interacting RNAs piRNAs are dynamically expressed for orchestrating the maternal to zygotic transition MZT. Systematic analysis of expression profiles of zebrafish small RNAome will be greatly helpful for understanding the sRNA regulation during embryonic development.", null, null, "embryonic development", "General Sample for zebrafish", "1 cell", null, "breed:wild type zebrafish", null, null, null, null, null, null, null, null, "1 cell developmental stage of zebrafish", "1 cell", "1", "Zebrafish embryos were collected at 1 cell 0.2 hpf  16 cell 1.5 hpf  512 cell 2.75 hpf  oblong 3.7 hpf  5.3 hpf 50% epibody  6 somite 12 hpf  24 hpf day1 hpf and 48 hpf day2 stages. Total RNA from embryos was isolated using Trizol reagent Invitrogen. RNAs were fractioned on 15% denaturing polyacrylamide gels  and small RNAs were isolated and purified. Subsequently  small RNAs were ligated with both a 5\u2019 adapter and 3\u2019 adapter for reverse transcription using SuperscriptTM II reverse transcription kit Invitrogen following the manufacturer's instructions at 42 oC for 1 h and 70 oC for 15 min. post that  the reverse transcribed product  cDNA was amplified by the following PCR program: a 15 cycle reaction at 98 oC for 30 sec  followed by 15 cycles consisting of 10 sec at 98 oC  15 sec at 72 oC  and then 10 min at 72 oC. post obtaining a 92bp DNA band on 6% denaturing PAGE gels  the PCR products were enriched by ethanol precipitation and purified using Spin X filter columns Fisher.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "size fractionation", "SINGLE", "ILLUMINA", "Illumina HiSeq 2000", "<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>44</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>", "SRP028754", null, null, "s1-cell.fq", "fastq", 1002077048.0, 20450552.0, "1 cell", "0:49 1:0", "A:210178307;C:226566574;G:255003953;T:310259058;N:69156", 49, 0, null, null, 210178307, 226566574, 255003953, 310259058, 69156, "SRX334986", "SRS469877", "SRA097312", "Huazhong University of Science and Technology|CUCKOO", "Huazhong University of Science and Technology", 1, 0.0001, null, 3e-05, null, 0.99977, null, 0.58333, null, 49, null, "T", null, "under 1.2% mapping rate", "illumina", "hiseq_era", "unknown", "size_fractionation", "unknown", "bulk", "unknown", "unknown", null, "China", "2014-08-12", "Multi-stage", "Embryo", "Embryo Imprecise", "All anatomical structures"]], "columns": ["rowid", "run.accession", "experiment.accession", "sample.accession", "study.accession", "bioproject", "study.title", "study.alias", "study.type", "study.abstract", "study.attributes", "study.PMIDs", "sample.description", "sample.title", "sample.alias", "sample.centername", "sample.attributes", "GEOsample.title", "GEOsample.dataprocessing", "GEOsample.source", "GEOsample.treatmentprotocol", "GEOsample.extractprotocol", "GEOsample.growthprotocol", "GEOsample.characteristics", "GEOsample.accession", "experiment.title", "experiment.alias", "experiment.library_name", "experiment.design_description", "experiment.library_construction_protocol", "experiment.attributes", "experiment.library_strategy", "experiment.library_source", "experiment.library_selection", "experiment.library_layout", "experiment.platform", "experiment.instrument_model", "experiment.spot_descriptor", "experiment.study_ref", "run.title", "run.attributes", "run.filename", "run.semantic_name", "run.total_bases", "run.total_spots", "run.alias", "run.read_lengths", "run.base_counts", "run.r1_length", "run.r2_length", "run.r3_length", "run.r4_length", "run.Acount", "run.Ccount", "run.Gcount", "run.Tcount", "run.Ncount", "run.experiment", "run.pool_member", "submission.accession", "submission.srasource", "submission.bioprojectsource", "seqdetective.n_mates", "seqdetective.mapping_rate.mate1", "seqdetective.mapping_rate.mate2", "seqdetective.nofeature_rate.mate1", "seqdetective.nofeature_rate.mate2", "seqdetective.sparsity.mate1", "seqdetective.sparsity.mate2", "seqdetective.pos_strand_rate.mate1", "seqdetective.pos_strand_rate.mate2", "seqdetective.readlen.mate1", "seqdetective.readlen.mate2", "seqdetective.judgement.mate1", "seqdetective.judgement.mate2", "seqdetective.judgement.reason", "platform_family", "instrument_generation", "read_bias", "selection_class", "prep_kit", "sc_or_bulk", "tech_class", "technology", "tech_variant", "submission.bioprojectsource.country", "earliest_date", "devstage_curation", "devstage_curation_coarse", "tissue_curation", "tissue_curation_coarse"], "primary_keys": ["rowid"], "primary_key_values": ["37121"], "units": {}, "query_ms": 12.367944000288844}