{"database": "metadata", "table": "run_metadata", "rows": [[36286, "SRR363984", "SRX105297", "SRS270140", "SRP009275", "PRJNA148581", "Hen1 analysis in zebrafish", "GSE33582", "Transcriptome Analysis", "small RNA libraries from wild type and Hen1 mutant testes were made with either polyA tailing VASAGFPHen1minus/plus or adapter ligation Hen1Testis and WTTestis and sequenced on an Illumina GAII platform. Overall design: RNA was isolated from total testis tissue of both Hen1 wildtype and Hen1 mutant animals. post size selection from gel  the small RNA libraries wre made.", null, "pubmed:20859253", null, "hen1 mutant ligation", "GSM830246", null, "source name:testis|strain:TL|genotype/variation:Hen1 mutant|tissue:testis|small rna library prep method:adapter ligation", "hen1 mutant ligation", "three prime adapter sequences were trimmed and inserts longer than 18 nt were mapped to the zebrafish genome Zv8", "testis", null, "Small RNAs in the size range of 19 31 bases were excised from a denaturing gel. Adaptors were ligated to the five prime and three prime ends of the isolated RNA  and the product was converted to cDNA using a primer on the three prime adaptor. post 15 cycles PCR amplification of the library the product was gel purified and sequenced on a Solexa platform.", null, "strain:TL|genotype/variation:Hen1 mutant|tissue:testis|small rna library prep method:adapter ligation", "GSM830246", "GSM830246: hen1 mutant ligation", "GSM830246: hen1 mutant ligation", "GSM830246: hen1 mutant ligation", "1", null, "GEO Accession:GSM830246", "RNA-Seq", "TRANSCRIPTOMIC", "other", "SINGLE", "ILLUMINA", "Illumina Genome Analyzer II", "<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>46</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>", "SRP009275", null, "read name barcode proc directive:ignore", "HEN1TESTIS.fastq", "fastq", 440876374.0, 9584269.0, "GSM830246 1", "0:46", "A:91693980;C:99515953;G:105634029;T:143841954;N:190458", 46, null, null, null, 91693980, 99515953, 105634029, 143841954, 190458, "SRX105297", "SRS270140", "SRA047996", "GEO", "European Research Institute for the Biology of Ageing, University Medical Center Groningen", 1, 0.00039, null, 0.00033, null, 0.99995, null, 0.0, null, 46, null, "T", null, "under 1.2% mapping rate", "illumina", "early_illumina", "5prime", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "Netherlands", "2011-11-09", "Undetermined", "Undetermined", "Gonad", "Reproductive System"]], "columns": ["rowid", "run.accession", "experiment.accession", "sample.accession", "study.accession", "bioproject", "study.title", "study.alias", "study.type", "study.abstract", "study.attributes", "study.PMIDs", "sample.description", "sample.title", "sample.alias", "sample.centername", "sample.attributes", "GEOsample.title", "GEOsample.dataprocessing", "GEOsample.source", "GEOsample.treatmentprotocol", "GEOsample.extractprotocol", "GEOsample.growthprotocol", "GEOsample.characteristics", "GEOsample.accession", "experiment.title", "experiment.alias", "experiment.library_name", "experiment.design_description", "experiment.library_construction_protocol", "experiment.attributes", "experiment.library_strategy", "experiment.library_source", "experiment.library_selection", "experiment.library_layout", "experiment.platform", "experiment.instrument_model", "experiment.spot_descriptor", "experiment.study_ref", "run.title", "run.attributes", "run.filename", "run.semantic_name", "run.total_bases", "run.total_spots", "run.alias", "run.read_lengths", "run.base_counts", "run.r1_length", "run.r2_length", "run.r3_length", "run.r4_length", "run.Acount", "run.Ccount", "run.Gcount", "run.Tcount", "run.Ncount", "run.experiment", "run.pool_member", "submission.accession", "submission.srasource", "submission.bioprojectsource", "seqdetective.n_mates", "seqdetective.mapping_rate.mate1", "seqdetective.mapping_rate.mate2", "seqdetective.nofeature_rate.mate1", "seqdetective.nofeature_rate.mate2", "seqdetective.sparsity.mate1", "seqdetective.sparsity.mate2", "seqdetective.pos_strand_rate.mate1", "seqdetective.pos_strand_rate.mate2", "seqdetective.readlen.mate1", "seqdetective.readlen.mate2", "seqdetective.judgement.mate1", "seqdetective.judgement.mate2", "seqdetective.judgement.reason", "platform_family", "instrument_generation", "read_bias", "selection_class", "prep_kit", "sc_or_bulk", "tech_class", "technology", "tech_variant", "submission.bioprojectsource.country", "earliest_date", "devstage_curation", "devstage_curation_coarse", "tissue_curation", "tissue_curation_coarse"], "primary_keys": ["rowid"], "primary_key_values": ["36286"], "units": {}, "query_ms": 8.766619001107756}