{"database": "metadata", "table": "run_metadata", "rows": [[36212, "SRR33570992", "SRX28800977", "SRS25038275", "SRP585332", "PRJNA1262902", "BCAS2 promotes primitive hematopoiesis by sequestering \u00df catenin within the nucleus", "GSE297155", "Transcriptome Analysis", "Breast carcinoma amplified sequence 2 BCAS2  a core component of the hPrP19 complex  plays crucial roles in various physiological and pathological processes. However  whether BCAS2 has functions other than being a key RNA splicing regulator within the nucleus remains unknown. Here  we show that BCAS2 is essential for primitive hematopoiesis in zebrafish and mouse embryos. The activation of Wnt/\u00df catenin signal  which is required for hematopoietic progenitor differentiation  is significantly decreased upon depletion of bcas2 in zebrafish embryos and mouse embryonic fibroblasts MEFs. Interestingly  BCAS2 de?ciency has no obvious impact on the splicing efficiency of \u00df catenin pre mRNA  while significantly attenuating \u00df catenin nuclear accumulation. Moreover  we find that BCAS2 directly binds to \u00df catenin via its coiled coil domains  thereby sequestering \u00df catenin within the nucleus. Thus  our results uncover a previously unknown function of BCAS2 in promoting Wnt signaling by enhancing \u00df catenin nuclear retention during primitive hematopoiesis. Overall design: RNA sequencing profiling for wild type and bcas2 +/?14 embryos at 10 ss.", null, null, null, "b14", "GSM8984757", null, "source name:embryo|tissue:embryo|genotype:heterozygous|geo loc name:missing|collection date:missing", "b14", "The difference in the number of alternative splicing events between groups was analyzed using rMATS version 4.1.0 Assembly: GRCz11 Supplementary files format and content: Gene expression matrix", "embryo", null, "Embryos were collected at the 10 somite stage and gently transferred into lysis buffer. Reverse transcription was performed using a SMARTer Ultra Low RNA Kit Clontech  634437 directly from the cell lysates. The cDNA library was prepared using an Advantage 2 PCR Kit Clontech\uff0c639206 and then sequenced via the Illumina sequencing platform NovaSeq 6000.", null, "tissue:embryo|genotype:heterozygous", "GSM8984757", "GSM8984757: b14; Danio rerio; RNA Seq", "GSM8984757 r1", "GSM8984757", "1", "Embryos were collected at the 10 somite stage and gently transferred into lysis buffer. Reverse transcription was performed using a SMARTer Ultra Low RNA Kit Clontech  634437 directly from the cell lysates. The cDNA library was prepared using an Advantage 2 PCR Kit Clontech\uff0c639206 and then sequenced via the Illumina sequencing platform NovaSeq 6000.", null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP585332", null, null, "b14_1.fq.gz b14_2.fq.gz", "fastq fastq", 19276565400.0, 64255218.0, "GSM8984757 r1", "0:150 1:150", "A:5518694219;C:3928662779;G:4574767411;T:5253904649;N:536342", 150, 150, null, null, 5518694219, 3928662779, 4574767411, 5253904649, 536342, "SRX28800977", "SRS25038275", "SRA2129895", "Affiliated Hospital of Guangdong Medical University", "Affiliated Hospital of Guangdong Medical University", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "full_length", "cdna_unspecified", "smarter", "bulk", "unknown", "unknown", null, "China", "2025-05-14", "Segmentation", "Embryo", "Embryo Imprecise", "All anatomical structures"]], "columns": ["rowid", "run.accession", "experiment.accession", "sample.accession", "study.accession", "bioproject", "study.title", "study.alias", "study.type", "study.abstract", "study.attributes", "study.PMIDs", "sample.description", "sample.title", "sample.alias", "sample.centername", "sample.attributes", "GEOsample.title", "GEOsample.dataprocessing", "GEOsample.source", "GEOsample.treatmentprotocol", "GEOsample.extractprotocol", "GEOsample.growthprotocol", "GEOsample.characteristics", "GEOsample.accession", "experiment.title", "experiment.alias", "experiment.library_name", "experiment.design_description", "experiment.library_construction_protocol", "experiment.attributes", "experiment.library_strategy", "experiment.library_source", "experiment.library_selection", "experiment.library_layout", "experiment.platform", "experiment.instrument_model", "experiment.spot_descriptor", "experiment.study_ref", "run.title", "run.attributes", "run.filename", "run.semantic_name", "run.total_bases", "run.total_spots", "run.alias", "run.read_lengths", "run.base_counts", "run.r1_length", "run.r2_length", "run.r3_length", "run.r4_length", "run.Acount", "run.Ccount", "run.Gcount", "run.Tcount", "run.Ncount", "run.experiment", "run.pool_member", "submission.accession", "submission.srasource", "submission.bioprojectsource", "seqdetective.n_mates", "seqdetective.mapping_rate.mate1", "seqdetective.mapping_rate.mate2", "seqdetective.nofeature_rate.mate1", "seqdetective.nofeature_rate.mate2", "seqdetective.sparsity.mate1", "seqdetective.sparsity.mate2", "seqdetective.pos_strand_rate.mate1", "seqdetective.pos_strand_rate.mate2", "seqdetective.readlen.mate1", "seqdetective.readlen.mate2", "seqdetective.judgement.mate1", "seqdetective.judgement.mate2", "seqdetective.judgement.reason", "platform_family", "instrument_generation", "read_bias", "selection_class", "prep_kit", "sc_or_bulk", "tech_class", "technology", "tech_variant", "submission.bioprojectsource.country", "earliest_date", "devstage_curation", "devstage_curation_coarse", "tissue_curation", "tissue_curation_coarse"], "primary_keys": ["rowid"], "primary_key_values": ["36212"], "units": {}, "query_ms": 11.412625004595611}