{"database": "metadata", "table": "run_metadata", "rows": [[34659, "SRR32162988", "SRX27508419", "SRS23927741", "SRP560539", "PRJNA1216901", "Impaired detoxification of Acrolein interrupted ocular vascular integrity and kidney structure in akr7a3 /  zebrafish via enhancing Arachidonic acid/leukotriene metabolism", "GSE288313", "Transcriptome Analysis", "Acrolein ACR is an endogenous reactive unsaturated aldehyde that can be detoxified by the aldo keto reductase AKR enzyme system. The accumulation of ACR is associated with several health issues  including inflammation  oxidative stress  and cardiovascular diseases. In this study  an akr7a3 mutant zebrafish with the Tgfli1: EGFP signature was generated to investigate the effect of ACR on vascular integrity. Elevated ACR levels were observed in akr7a3 /  zebrafish larvae and adults. Subsequent experiments demonstrated that increased ACR induced an enlargement of the hyaloid and retinal vasculature  as well as alterations in the larvae pronephron and adult kidney. Transcriptome and metabolomics analyses  followed by validation experiments  revealed that the upregulation of arachidonic acid metabolism and leukotriene production are responsible for the observed vascular and organ changes. In conclusion  our data suggests that the loss of akr7a3 in zebrafish impairs the detoxification of ACR  which subsequently disrupts vascular integrity and normal kidney structure by promoting an inflammatory response. Overall design: akr7a3 / zebrafish were generated using CRISPR/Cas9 technology.  Multiple experiments are performed regarding vasculature alterations  transcriptome  and metabolomics in Tgfli1:EGFP zebrafish.", null, "pubmed:40258306", null, "zf akr7a3 hom8", "GSM8764270", null, "source name:eye|tissue:eye|genotype:akr7a3 / |geo loc name:missing|collection date:missing", "zf akr7a3 hom8", "Raw sequence data with adapter sequences or low quality sequences was filtered. Filtered reads were aligned to the Zebrafish reference genome GRCz11 Raw counts levels were quantified using FeatureCounts v2.0.3 Assembly: GRCz11 Supplementary files format and content: tab delimited text file includes raw counts for each sample.", "eye", null, "Total RNA was extracted using RNeasy Mini KitQIAGEN  3ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA seq were prepared using DNBSEQ Eukaryotic Strand specific mRNA library.", null, "tissue:eye|genotype:akr7a3 / ", "GSM8764270", "GSM8764270: zf akr7a3 hom8; Danio rerio; RNA Seq", "GSM8764270 r1", "GSM8764270", "1", "Total RNA was extracted using RNeasy Mini KitQIAGEN  3ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA seq were prepared using DNBSEQ Eukaryotic Strand specific mRNA library.", null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "DNBSEQ", "DNBSEQ-G400", null, "SRP560539", null, null, "h8_1.fq.gz h8_2.fq.gz", "fastq fastq", 4842768400.0, 24213842.0, "GSM8764270 r1", "0:100 1:100", "A:1306148372;C:1113721147;G:1128251057;T:1294647824;N:0", 100, 100, null, null, 1306148372, 1113721147, 1128251057, 1294647824, 0, "SRX27508419", "SRS23927741", "SRA2064109", "Medical Faculty Mannheim", "Medical Faculty Mannheim", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "bgi", "bgi", "unknown", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "Germany", "2025-01-29", "Undetermined", "Larval", "Eye", "Sensory System"]], "columns": ["rowid", "run.accession", "experiment.accession", "sample.accession", "study.accession", "bioproject", "study.title", "study.alias", "study.type", "study.abstract", "study.attributes", "study.PMIDs", "sample.description", "sample.title", "sample.alias", "sample.centername", "sample.attributes", "GEOsample.title", "GEOsample.dataprocessing", "GEOsample.source", "GEOsample.treatmentprotocol", "GEOsample.extractprotocol", "GEOsample.growthprotocol", "GEOsample.characteristics", "GEOsample.accession", "experiment.title", "experiment.alias", "experiment.library_name", "experiment.design_description", "experiment.library_construction_protocol", "experiment.attributes", "experiment.library_strategy", "experiment.library_source", "experiment.library_selection", "experiment.library_layout", "experiment.platform", "experiment.instrument_model", "experiment.spot_descriptor", "experiment.study_ref", "run.title", "run.attributes", "run.filename", "run.semantic_name", "run.total_bases", "run.total_spots", "run.alias", "run.read_lengths", "run.base_counts", "run.r1_length", "run.r2_length", "run.r3_length", "run.r4_length", "run.Acount", "run.Ccount", "run.Gcount", "run.Tcount", "run.Ncount", "run.experiment", "run.pool_member", "submission.accession", "submission.srasource", "submission.bioprojectsource", "seqdetective.n_mates", "seqdetective.mapping_rate.mate1", "seqdetective.mapping_rate.mate2", "seqdetective.nofeature_rate.mate1", "seqdetective.nofeature_rate.mate2", "seqdetective.sparsity.mate1", "seqdetective.sparsity.mate2", "seqdetective.pos_strand_rate.mate1", "seqdetective.pos_strand_rate.mate2", "seqdetective.readlen.mate1", "seqdetective.readlen.mate2", "seqdetective.judgement.mate1", "seqdetective.judgement.mate2", "seqdetective.judgement.reason", "platform_family", "instrument_generation", "read_bias", "selection_class", "prep_kit", "sc_or_bulk", "tech_class", "technology", "tech_variant", "submission.bioprojectsource.country", "earliest_date", "devstage_curation", "devstage_curation_coarse", "tissue_curation", "tissue_curation_coarse"], "primary_keys": ["rowid"], "primary_key_values": ["34659"], "units": {}, "query_ms": 10.126174995093606}