{"database": "metadata", "table": "run_metadata", "rows": [[34606, "SRR32129952", "SRX27476251", "SRS23900300", "SRP559953", "PRJNA1215813", "Comparative Toxicological Effects of Tire Wear and Latex Particle Leachates in Zebrafish Embryos: Focus on Oxidative Stress and Ferroptosis", "PRJNA1215813", "Other", "Microrubber  a subset of microplastics  has emerged as a significant environmental concern due to its persistence  bioaccumulation  and potential toxicity in aquatic ecosystems. This study investigates the toxicological effects of leachates derived from tire wear particles TWP and latex particles LAP on zebrafish embryos  focusing on physiological  oxidative stress  and transcriptomic responses. LAP leachate exhibited significantly higher toxicity than TWP  characterized by increased mortality  delayed hatching  reduced spontaneous movement  suppressed heart rate  and severe morphological malformations. Chemical analysis identified elevated levels of heavy metals and biologically active organic compounds  with higher zinc concentrations and benzothiazole derivatives in LAP leachate contributing to its greater toxicity. Oxidative stress markers revealed elevated catalase CAT and malondialdehyde MDA levels in both groups  but LAP exposure significantly reduced glutathione S transferase GST activity  indicating compromised detoxification capacity. Transcriptomic analysis identified ferroptosis as a central pathway mediating toxicity in both leachates. LAP exposure was associated with the upregulation of mt2 and fthl31 and the downregulation of slc40a1  suggesting disrupted iron metabolism and exacerbated oxidative damage. In contrast  TWP exposure triggered adaptive responses  including the upregulation of detoxification related genes such as cyp1a and gstt1b. These findings elucidate distinct toxicity mechanisms between TWP and LAP leachates and underscore the need for enhanced environmental monitoring and regulatory strategies to mitigate their ecological impacts.", null, null, null, null, "LAP2", null, "breed:AB|age:5 days|collection date:2024 05 26|geo loc name:Not collected|sex:Missing|tissue:Whole body|Replicate:Replicate = biological replicate 8|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of danio rerio: zebrafish embryos", "LAP2", "LAP2", "mRNA seq of Danio rerio  relication 2 of LAP", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PCR", "PAIRED", "ILLUMINA", "Illumina NovaSeq X Plus", null, "SRP559953", null, null, "SLAP2.R1.raw.fastq.gz SLAP2.R2.raw.fastq.gz", "fastq fastq", 7139867692.0, 23641946.0, "SLAP2.R1.raw.fastq.gz", "0:151 1:151", "A:1904886971;C:1644487548;G:1698548524;T:1891760900;N:183749", 151, 151, null, null, 1904886971, 1644487548, 1698548524, 1891760900, 183749, "SRX27476251", "SRS23900300", "SRA2060932", "Qingdao University of Science and Technology|College of Marine Science and biological engineeri", "Qingdao University of Science and Technology", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2025-01-26", "Larval", "Larval", "Trunk", "Surface Structure"]], "columns": ["rowid", "run.accession", "experiment.accession", "sample.accession", "study.accession", "bioproject", "study.title", "study.alias", "study.type", "study.abstract", "study.attributes", "study.PMIDs", "sample.description", "sample.title", "sample.alias", "sample.centername", "sample.attributes", "GEOsample.title", "GEOsample.dataprocessing", "GEOsample.source", "GEOsample.treatmentprotocol", "GEOsample.extractprotocol", "GEOsample.growthprotocol", "GEOsample.characteristics", "GEOsample.accession", "experiment.title", "experiment.alias", "experiment.library_name", "experiment.design_description", "experiment.library_construction_protocol", "experiment.attributes", "experiment.library_strategy", "experiment.library_source", "experiment.library_selection", "experiment.library_layout", "experiment.platform", "experiment.instrument_model", "experiment.spot_descriptor", "experiment.study_ref", "run.title", "run.attributes", "run.filename", "run.semantic_name", "run.total_bases", "run.total_spots", "run.alias", "run.read_lengths", "run.base_counts", "run.r1_length", "run.r2_length", "run.r3_length", "run.r4_length", "run.Acount", "run.Ccount", "run.Gcount", "run.Tcount", "run.Ncount", "run.experiment", "run.pool_member", "submission.accession", "submission.srasource", "submission.bioprojectsource", "seqdetective.n_mates", "seqdetective.mapping_rate.mate1", "seqdetective.mapping_rate.mate2", "seqdetective.nofeature_rate.mate1", "seqdetective.nofeature_rate.mate2", "seqdetective.sparsity.mate1", "seqdetective.sparsity.mate2", "seqdetective.pos_strand_rate.mate1", "seqdetective.pos_strand_rate.mate2", "seqdetective.readlen.mate1", "seqdetective.readlen.mate2", "seqdetective.judgement.mate1", "seqdetective.judgement.mate2", "seqdetective.judgement.reason", "platform_family", "instrument_generation", "read_bias", "selection_class", "prep_kit", "sc_or_bulk", "tech_class", "technology", "tech_variant", "submission.bioprojectsource.country", "earliest_date", "devstage_curation", "devstage_curation_coarse", "tissue_curation", "tissue_curation_coarse"], "primary_keys": ["rowid"], "primary_key_values": ["34606"], "units": {}, "query_ms": 12.9101089987671}