{"database": "metadata", "table": "run_metadata", "rows": [[34060, "SRR31034585", "SRX26420026", "SRS22939658", "SRP539240", "PRJNA1174234", "Organogenesis and replication phenoypes in vivo suggest distinct effects of hypusinated and unhypusinated eIF5A", "GSE279773", "Transcriptome Analysis", "Prior to type 2 diabetes onset  \u00df cells adapt to insulin resistance through compensation\u2014a process that maintains insulin secretion and glucose homeostasis. Our lab has previously shown that \u00df cell compensation requires the activity of deoxyhypusine synthase DHPS  which post translationally catalyzes the formation of the amino acid hypusine at Lys50 of eukaryotic initiation factor eIF5A. Although hypusinated eIF5A is required for \u00df cell compensation  it is unclear if unhypusinated eIF5A limits this compensatory response. To identify the role of unhypusinated eIF5A  we used the following animal and cell based models: transgenic zebrafish and inducible  \u00df cell specific knockout mice fed a high fat diet. Zebrafish embryos injected with morpholinos to reduce global DHPS and accumulate unhypusinated eIF5A showed stunted exocrine pancreas growth at xxx dpf Although  those injected with anti eIF5A morpholinos to deplete all eIF5A showed normal pancreas growth. Although a unique function of unhypusinated eIF5A has not yet been documented  these findings suggest that the presence of unhypusinated eIF5A may be the major driver of altered pancreas phenotypes. Similarly  following 4 weeks of high fat diet feeding and obesity  mice lacking total eIF5A in \u00df cells had improved glucose tolerance compared to mice lacking DHPS in \u00df cells  despite similar weight gain and insulin sensitivity. Taken together  our data provide evidence that DHPS deficiency and obesity conditions impair \u00df cell function  inpart  from the accumulation of the unhypusinated form of eIF5A. Our studies reveal a mechanism in which \u00df cells respond to obesity by regulating mRNA translation through the balance between hypusinated and unhypusinated forms of eIF5A. Overall design: Zebrafish embryos were injected with control  dhps  or eif5a1/2 morpholinos; 24 hpf embryoes were processed for RNA and sent to sequencing.", null, "pubmed:39832654", null, "Control morpholino 2", "GSM8579956", null, "source name:whole body|tissue:whole body|treatment:Control morpholino|geo loc name:missing|collection date:missing", "Control morpholino 2", "Data was analyzed using Dr. Tom Assembly: GRCz11 Supplementary files format and content: excel file with tpm and read counts Supplementary files format and content: excel file with fold change and q value", "whole body", "zebrafish were treated with morpholino and RNA was extracted 24 hours later", "RNA was extracted using a trizol chloroform protocol mRNA enrichment was performed on total RNA using oligodT attached magnetic beads. The enriched mRNA with polyA tails was fragmented using a fragmentation buffer  followed by reverse transcription using random N6 primers to synthesize cDNA double strands. The synthesized double stranded DNA was then end repaired and five prime phosphorylated  with a protruding 'A' at the three prime end forming a blunt end  followed by ligation of a bubble shaped adapter with a protruding 'T' at the three primeend. The ligation products were PCR amplified using specific primers. The PCR products were denatured to single strands  and then single stranded circular DNA libraries were generated using a bridged primer. The constructed libraries were quality checked and sequenced post passing the quality control. mRNA enrichment was performed on total RNA using oligodT attached magnetic beads. The enriched mRNA with polyA tails was fragmented using a fragmentation buffer  followed by reverse transcription using random N6 primers to synthesize cDNA double strands. The synthesized double stranded DNA was then end repaired and five prime phosphorylated  with a protruding 'A' at the three prime end forming a blunt end  followed by ligation of a bubble shaped adapter with a protruding 'T' at the three primeend. The ligation products were PCR amplified using specific primers. The PCR products were denatured to single strands  and then single stranded circular DNA libraries were generated using a bridged primer. The constructed libraries were quality checked and sequenced post passing the quality control.", null, "tissue:whole body|treatment:Control morpholino", "GSM8579956", "GSM8579956: Control morpholino 2; Danio rerio; RNA Seq", "GSM8579956 r1", "GSM8579956", "1", "RNA was extracted using a trizol chloroform protocol mRNA enrichment was performed on total RNA using oligodT attached magnetic beads. The enriched mRNA with polyA tails was fragmented using a fragmentation buffer  followed by reverse transcription using random N6 primers to synthesize cDNA double strands. The synthesized double stranded DNA was then end repaired and five prime phosphorylated  with a protruding 'A' at the three prime end forming a blunt end  followed by ligation of a bubble shaped adapter with a protruding 'T' at the three primeend. The ligation products were PCR amplified using specific primers. The PCR products were denatured to single strands  and then single stranded circular DNA libraries were generated using a bridged primer. The constructed libraries were quality checked and sequenced post passing the quality control. mRNA enrichment was performed on total RNA using oligodT attached magnetic beads. The enriched mRNA with polyA tails was fragmented using a fragmentation buffer  followed by reverse transcription using random N6 primers to synthesize cDNA double strands. The synthesized double stranded DNA was then end repaired and five prime phosphorylated  with a protruding 'A' at the three prime end forming a blunt end  followed by ligation of a bubble shaped adapter with a protruding 'T' at the three primeend. The ligation products were PCR amplified using specific primers. The PCR products were denatured to single strands  and then single stranded circular DNA libraries were generated using a bridged primer. The constructed libraries were quality checked and sequenced post passing the quality control.", null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "DNBSEQ", "DNBSEQ-G400", null, "SRP539240", null, null, "CtrlMO2_1.fq.gz CtrlMO2_2.fq.gz", "fastq fastq", 6823000500.0, 22743335.0, "GSM8579956 r1", "0:150 1:150", "A:1777305843;C:1633379395;G:1649025317;T:1763289945;N:0", 150, 150, null, null, 1777305843, 1633379395, 1649025317, 1763289945, 0, "SRX26420026", "SRS22939658", "SRA1993170", "Medicine, University of Chicago", "Medicine, University of Chicago", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "bgi", "bgi", "3prime", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-10-17", "Undetermined", "Embryo", "Trunk", "Surface Structure"]], "columns": ["rowid", "run.accession", "experiment.accession", "sample.accession", "study.accession", "bioproject", "study.title", "study.alias", "study.type", "study.abstract", "study.attributes", "study.PMIDs", "sample.description", "sample.title", "sample.alias", "sample.centername", "sample.attributes", "GEOsample.title", "GEOsample.dataprocessing", "GEOsample.source", "GEOsample.treatmentprotocol", "GEOsample.extractprotocol", "GEOsample.growthprotocol", "GEOsample.characteristics", "GEOsample.accession", "experiment.title", "experiment.alias", "experiment.library_name", "experiment.design_description", "experiment.library_construction_protocol", "experiment.attributes", "experiment.library_strategy", "experiment.library_source", "experiment.library_selection", "experiment.library_layout", "experiment.platform", "experiment.instrument_model", "experiment.spot_descriptor", "experiment.study_ref", "run.title", "run.attributes", "run.filename", "run.semantic_name", "run.total_bases", "run.total_spots", "run.alias", "run.read_lengths", "run.base_counts", "run.r1_length", "run.r2_length", "run.r3_length", "run.r4_length", "run.Acount", "run.Ccount", "run.Gcount", "run.Tcount", "run.Ncount", "run.experiment", "run.pool_member", "submission.accession", "submission.srasource", "submission.bioprojectsource", "seqdetective.n_mates", "seqdetective.mapping_rate.mate1", "seqdetective.mapping_rate.mate2", "seqdetective.nofeature_rate.mate1", "seqdetective.nofeature_rate.mate2", "seqdetective.sparsity.mate1", "seqdetective.sparsity.mate2", "seqdetective.pos_strand_rate.mate1", "seqdetective.pos_strand_rate.mate2", "seqdetective.readlen.mate1", "seqdetective.readlen.mate2", "seqdetective.judgement.mate1", "seqdetective.judgement.mate2", "seqdetective.judgement.reason", "platform_family", "instrument_generation", "read_bias", "selection_class", "prep_kit", "sc_or_bulk", "tech_class", "technology", "tech_variant", "submission.bioprojectsource.country", "earliest_date", "devstage_curation", "devstage_curation_coarse", "tissue_curation", "tissue_curation_coarse"], "primary_keys": ["rowid"], "primary_key_values": ["34060"], "units": {}, "query_ms": 9.656453999923542}