{"database": "metadata", "table": "run_metadata", "rows": [[32117, "SRR29012743", "SRX24539425", "SRS21284302", "SRP507393", "PRJNA1111146", "RNA seq of adult zebrafish brain following traumatic brain injury and/or ISRIB exposure", "PRJNA1111146", "Other", "Here analysed gene expression changes post Traumatic Brain Injury TBI and subsequent exposure to ISRIB in the brain of adult Danio rerio. 4 groups are available: control C  traumatic brain injury TBI; T  ISRIB injection I TBI and ISRIB injection TI.", null, null, null, null, "TI5 TK5", null, "strain:Wild type|age:5 month|collection date:2023 08 20|geo loc name:Russia|sex:NA|tissue:brain|sample type:TI5 TK5|treatment:TBI + ISRIB|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Here analysed gene expression changes post Traumatic Brain Injury TBI and subsequent exposure to ISRIB in the brain of adult Danio rerio. 4 groups are available: control C  traumatic brain injury TBI; T  ISRIB injection I TBI and ISRIB injection TI.", "TK5", "TK5", "RNA isolation was performed using the ExtractRNA reagent following the standard protocol for TriZol mediated extraction. The RNA quality was checked by capillary electrophoresis using the QIAxcel Advanced program. Total RNA was used for isolation of polyA fraction using NEBNext PolyA mRNA Magnetic Isolation Module according to the manufacturer's recommendations. RNA was next quantified on Quntus fluorometer and used for library preparation using NEBNext UltraTM Directional RNA Library PrepKit for Illumina with NEBNext Multiplex Oligos for Illumina according to the manufacturer's recommendations. The quality of libraries was confirmed by capillary electrophoresis using the QIAxcel Advanced program. Pair end sequencing was performed using Illumina HiSeq2500 with a read length of 110 bp.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA_oligo_dT", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP507393", null, null, "TI5-TK5_S4_R1_001.fastq.gz TI5-TK5_S4_R2_001.fastq.gz", "fastq fastq", 2048648256.0, 9524761.0, "TI5 TK5 S4 R1 001.fastq.gz", "0:107.53 1:107.56", "A:575845206;C:442981520;G:439368910;T:583602331;N:6850289", 107, 107, null, null, 575845206, 442981520, 439368910, 583602331, 6850289, "SRX24539425", "SRS21284302", "SRA1865594", "Sirius University of Science and Technology|Genetics and Life Science", "Sirius University of Science and Technology", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "nebnext", "bulk", "unknown", "unknown", null, "Russia", "2024-05-13", "Adult", "Adult", "Brain", "Nervous System"]], "columns": ["rowid", "run.accession", "experiment.accession", "sample.accession", "study.accession", "bioproject", "study.title", "study.alias", "study.type", "study.abstract", "study.attributes", "study.PMIDs", "sample.description", "sample.title", "sample.alias", "sample.centername", "sample.attributes", "GEOsample.title", "GEOsample.dataprocessing", "GEOsample.source", "GEOsample.treatmentprotocol", "GEOsample.extractprotocol", "GEOsample.growthprotocol", "GEOsample.characteristics", "GEOsample.accession", "experiment.title", "experiment.alias", "experiment.library_name", "experiment.design_description", "experiment.library_construction_protocol", "experiment.attributes", "experiment.library_strategy", "experiment.library_source", "experiment.library_selection", "experiment.library_layout", "experiment.platform", "experiment.instrument_model", "experiment.spot_descriptor", "experiment.study_ref", "run.title", "run.attributes", "run.filename", "run.semantic_name", "run.total_bases", "run.total_spots", "run.alias", "run.read_lengths", "run.base_counts", "run.r1_length", "run.r2_length", "run.r3_length", "run.r4_length", "run.Acount", "run.Ccount", "run.Gcount", "run.Tcount", "run.Ncount", "run.experiment", "run.pool_member", "submission.accession", "submission.srasource", "submission.bioprojectsource", "seqdetective.n_mates", "seqdetective.mapping_rate.mate1", "seqdetective.mapping_rate.mate2", "seqdetective.nofeature_rate.mate1", "seqdetective.nofeature_rate.mate2", "seqdetective.sparsity.mate1", "seqdetective.sparsity.mate2", "seqdetective.pos_strand_rate.mate1", "seqdetective.pos_strand_rate.mate2", "seqdetective.readlen.mate1", "seqdetective.readlen.mate2", "seqdetective.judgement.mate1", "seqdetective.judgement.mate2", "seqdetective.judgement.reason", "platform_family", "instrument_generation", "read_bias", "selection_class", "prep_kit", "sc_or_bulk", "tech_class", "technology", "tech_variant", "submission.bioprojectsource.country", "earliest_date", "devstage_curation", "devstage_curation_coarse", "tissue_curation", "tissue_curation_coarse"], "primary_keys": ["rowid"], "primary_key_values": ["32117"], "units": {}, "query_ms": 9.427322002011351}