{"database": "metadata", "table": "run_metadata", "rows": [[30775, "SRR28357669", "SRX23962905", "SRS20762620", "SRP495490", "PRJNA1088483", "Mechanistic Studies of the Regulation of Cardiomyocytes Spatial Distributions by Heterogeneous Erbb2 Expression [scRNA Seq]", "GSE261718", "Other", "Purpose: To explore the endogenous expression pattern of Erbb2. Methods:  Approximately 1000 hearts dissected from 72 hpf Tgmyl7:mCherry embryos were digested in 0.25% trypsin EDTA solution for single cell RNA sequencing. Results: We found that ventricular Erbb2 expression was highly enriched in subcluster 0 and 2  but weakly expressed in subcluster 3  10 and 12. Conclusions: Erbb2 exhibits heterogeneous expression during trabeculation. Overall design: 72 hpf Tgmyl7:mCherry zebrafish hearts were collected for single cell RNA sequencing.", null, "pubmed:40053597", null, "S72hpf trunks", "GSM8149579", null, "source name:zebrafish hearts|tissue:zebrafish hearts|cell line:Tgmyl7:mCherry heart cells|cell type:zebrafish heart cells|geo loc name:missing|collection date:missing", "S72hpf trunks", "Illumina sequencing reads were aligned to the zebrafish mRNA reference genome GRCz11 using the 10x Genomics CellRanger pipeline version 3.0.2 with default parameters. Assembly: GRCz11 Supplementary files format and content: zip compressed  files include filtered gene bc matrices post running CellRanger pipeline", "zebrafish hearts", null, "A total of 1000 hearts dissected from Tgmyl7:mCherry embryos at 72 hpf were digested in 0.25% trypsin EDTA solution at 37 \u00b0C for 30 min. Single cell RNA seq library construction was performed using a droplet based library prep platform Chromium 10x Genomics with a Chromium Single Cell Reagent Kit according to the manufacturer\u2019s instructions. Single cell RNA Seq 10x", "Dechorionated zebrafish embryos were maintained in 0.3x Danieau\u2019s Buffer at 28 \u00b0C with a photoperiod of 14/10\u2009h light/dark.", "tissue:zebrafish hearts|cell line:Tgmyl7:mCherry heart cells|cell type:zebrafish heart cells", "GSM8149579", "GSM8149579: S72hpf trunks; Danio rerio; RNA Seq", "GSM8149579 r1", "GSM8149579", "1", "A total of 1000 hearts dissected from Tgmyl7:mCherry embryos at 72 hpf were digested in 0.25% trypsin EDTA solution at 37 \u00b0C for 30 min. Single cell RNA seq library construction was performed using a droplet based library prep platform Chromium 10x Genomics with a Chromium Single Cell Reagent Kit according to the manufacturer's instructions. 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