{"database": "metadata", "table": "run_metadata", "rows": [[30280, "SRR27747456", "SRX23412764", "SRS20268085", "SRP486175", "PRJNA1069777", "Effect of phox2a knockout on the molecular profiles of hindbrain vestibular neurons in the larval zebrafish bulk RNA Seq", "GSE254345", "Transcriptome Analysis", "Sensorimotor reflex circuits engage distinct neuronal subtypes  defined by precise connectivity  to transform sensation into compensatory behavior. Whether and how motor partner populations shape the subtype fate and connectivity of their pre motor counterparts remains controversial. Here  we discovered that motor partners are dispensable for proper connectivity across an entire vestibular reflex circuit that stabilizes gaze. We first measured activity following vestibular sensation in pre motor projection neurons post constitutive loss of their extraocular motor neuron partners.We observed normal responses and topography consistent with unchanged functional connectivity between sensory neurons and projection neurons. Next  we show that projection neurons remain anatomically and molecularly poised to connect appropriately with their motor partners. Lastly  we show that the transcriptional signatures of projection neuron subtypes develop independently of motor partners. Our findings comprehensively overturn a long standing model: that connectivity in the circuit for gaze stabilization is retrogradely determined by motor partner derived signals. By defining the contribution of motor neurons to canonical sensorimotor circuit assembly  our work speaks to comparable processes in spinal circuits and advances our understanding of general principles of neural development. Overall design: Hindbrain vestibular neurons  labeled by Tg 6.7Tru.Hcrtr2:GAL4 VP16;TgUAS:E1b Kaede  harvested from zebrafish embryos between 72 hpf 74 hpf. Embyros were from two conditions: larvae from a stable line of phox2a /  mutants  and sibling controls phox2a+/+ or +/ . Fluorescent neurons were isolated by fluorescence activated cell sorting FACS according to Kaede fluorescence. Four experimental repeats were performed  each generating two samples phox2a /  and sibling control. Bulk RNA sequencing was performed.", null, null, null, "Hindbrain vestibular neurons  phox2a /   replicate 2", "GSM8038032", null, "tissue:Hindbrain vestibular neurons|time:72 hpf 74 hpf type:Hindbrain vestibular neurons|genotype:Tg 6.7Tru.Hcrtr2:GAL4 VP16; TgUAS:E1b Kaede; Tgisl1:GFP; phox2a / |geo loc name:missing|collection date:missing", "Hindbrain vestibular neurons  phox2a /   replicate 2", "DESeq2 Assembly: GRCz11 Supplementary files format and content: excel file includes raw counts for each sample Supplementary files format and content: excel file includes normalized counts for each sample", "Hindbrain vestibular neurons", null, "RNA was isolated using an RNAqueous Total RNA Isolation Kit. RNA quality and concentration was assessed using an RNA 6000 Pico Kit and a 2100 BioAnalyzer system. Libraries for bulk RNA sequencing were prepared using the low input Clontech SMART Seq HT with Nxt HT kit Takara", null, "time:72 hpf 74 hpf type:Hindbrain vestibular neurons|genotype:Tg 6.7Tru.Hcrtr2:GAL4 VP16; TgUAS:E1b Kaede; Tgisl1:GFP; phox2a / ", "GSM8038032", "GSM8038032: Hindbrain vestibular neurons  phox2a /   replicate 2; Danio rerio; RNA Seq", "GSM8038032 r1", "GSM8038032", "1", "RNA was isolated using an RNAqueous Total RNA Isolation Kit. RNA quality and concentration was assessed using an RNA 6000 Pico Kit and a 2100 BioAnalyzer system. Libraries for bulk RNA sequencing were prepared using the low input Clontech SMART Seq HT with Nxt HT kit Takara", null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP486175", null, "loader:fastq load.py", "1122_phox2a_S27_L002_R1_001.fastq.gz 1122_phox2a_S27_L002_R2_001.fastq.gz", "fastq fastq", 3449800956.0, 33821578.0, "GSM8038032 r1", "0:51 1:51", "A:1024161989;C:698197523;G:689509862;T:1037871222;N:60360", 51, 51, null, null, 1024161989, 698197523, 689509862, 1037871222, 60360, "SRX23412764", "SRS20268085", "SRA1792521", "Neuroscience Institute, New York University Grossman School of Medicine", "Neuroscience Institute, New York University Grossman School of Medicine", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_plate", "smartseq", null, "United States", "2024-01-26", "Larval", "Larval", "Brain", "Nervous System"]], "columns": ["rowid", "run.accession", "experiment.accession", "sample.accession", "study.accession", "bioproject", "study.title", "study.alias", "study.type", "study.abstract", "study.attributes", "study.PMIDs", "sample.description", "sample.title", "sample.alias", "sample.centername", "sample.attributes", "GEOsample.title", "GEOsample.dataprocessing", "GEOsample.source", "GEOsample.treatmentprotocol", "GEOsample.extractprotocol", "GEOsample.growthprotocol", "GEOsample.characteristics", "GEOsample.accession", "experiment.title", "experiment.alias", "experiment.library_name", "experiment.design_description", "experiment.library_construction_protocol", "experiment.attributes", "experiment.library_strategy", "experiment.library_source", "experiment.library_selection", "experiment.library_layout", "experiment.platform", "experiment.instrument_model", "experiment.spot_descriptor", "experiment.study_ref", "run.title", "run.attributes", "run.filename", "run.semantic_name", "run.total_bases", "run.total_spots", "run.alias", "run.read_lengths", "run.base_counts", "run.r1_length", "run.r2_length", "run.r3_length", "run.r4_length", "run.Acount", "run.Ccount", "run.Gcount", "run.Tcount", "run.Ncount", "run.experiment", "run.pool_member", "submission.accession", "submission.srasource", "submission.bioprojectsource", "seqdetective.n_mates", "seqdetective.mapping_rate.mate1", "seqdetective.mapping_rate.mate2", "seqdetective.nofeature_rate.mate1", "seqdetective.nofeature_rate.mate2", "seqdetective.sparsity.mate1", "seqdetective.sparsity.mate2", "seqdetective.pos_strand_rate.mate1", "seqdetective.pos_strand_rate.mate2", "seqdetective.readlen.mate1", "seqdetective.readlen.mate2", "seqdetective.judgement.mate1", "seqdetective.judgement.mate2", "seqdetective.judgement.reason", "platform_family", "instrument_generation", "read_bias", "selection_class", "prep_kit", "sc_or_bulk", "tech_class", "technology", "tech_variant", "submission.bioprojectsource.country", "earliest_date", "devstage_curation", "devstage_curation_coarse", "tissue_curation", "tissue_curation_coarse"], "primary_keys": ["rowid"], "primary_key_values": ["30280"], "units": {}, "query_ms": 10.583593000774272}