{"database": "metadata", "table": "run_metadata", "rows": [[11748, "ERR11608179", "ERX11010310", "ERS15939664", "ERP148707", "PRJEB63561", "RNA seq of Danio rerio wild type and kdm4b knockout mutant gata1+ erythroid progenitors and precursors", "E-MTAB-13116", "Transcriptome Analysis", "Transcriptomic differences of gata1+ erythrocytes between the kdm4b /  mutants and the WT embryos at 48 hpf were analyzed by RNA sequencing.", "ENA FIRST PUBLIC:2024 07 01|ENA LAST UPDATE:2024 07 01", null, "Protocols: The 48 hpf gata1+ erythrocytes were sorted FACSAria  BD Bioscience  CA  USA Two independent groups of RNA from wt and kdm4b /  erythrocytes were extracted using TRIzol\u00ae Reagent Life Technologies each group contains three biological repeats. post adenylation of three prime ends of double strand cDNA fragments  Adaptor with hairpin loop structure were ligated to prepare for hybridization. The library fragments were purified with AMPure XP system Beckman Coulter  Beverly  USA. Then PCR was performed with Phusion High Fidelity DNA polymerase. At last  PCR products were purified and the library quality was assessed on the Agilent Bioanalyzer 2100 system.", "WT1", "SAMEA113945414", "Shanghai Institute of Nutrition and Diseases, Chinese Academy of Sciences", "ENA first public:2024 07 01|External Id:SAMEA113945414|INSDC center name:Shanghai Institute of Nutrition and Diseases  Chinese Academy of Sciences|INSDC last update:2023 06 27T11:19:32Z|INSDC status:public|Submitter Id:E MTAB 13116:WT1|age:48|broker name:ArrayExpress|cell type:erythrocyte|collection date:not collected|common name:zebrafish|developmental stage:embryo stage|disease:normal|genotype:wild type genotype|geographic location country and/or sea:not collected|immunophenotype:gata1 positive|individual:mixed pool of 200 individuals|isolate:not applicable|organism part:embryo|sample name:E MTAB 13116:WT1|scientific name:Danio rerio|sex:mixed|strain:Tubingen", null, null, null, null, null, null, null, null, "RNA seq of Danio rerio wild type and kdm4b knockout mutant gata1+ erythroid progenitors and precursors", "E MTAB 13116:WT1 p", "WT1 p", "RNA seq of Danio rerio wild type and kdm4b knockout mutant gata1+ erythroid progenitors and precursors", "The 48 hpf gata1+ erythrocytes were sorted FACSAria  BD Bioscience  CA  USA Two independent groups of RNA from wt and kdm4b /  erythrocytes were extracted using TRIzol\u00ae Reagent Life Technologies each group contains three biological repeats. post adenylation of three prime ends of double strand cDNA fragments  Adaptor with hairpin loop structure were ligated to prepare for hybridization. The library fragments were purified with AMPure XP system Beckman Coulter  Beverly  USA. Then PCR was performed with Phusion High Fidelity DNA polymerase. 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