{"database": "metadata", "table": "run_metadata", "rows": [[11745, "ERR11422848", "ERX10830019", "ERS15422303", "ERP147133", "PRJEB62042", "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "E-MTAB-12934", "Transcriptome Analysis", "RNA seq of zebrafish embryos with mutations in srpk3 sa1890 allele and/or ttn.1 sa5562 allele. Each of the 24 samples six samples for each of four genotypes represents RNA from a pool of three 5 dpf zebrafish embryos.", "ENA FIRST PUBLIC:2024 02 09|ENA LAST UPDATE:2024 02 09", null, "Protocols: Zebrafish embryos were collected for DNA and RNA extraction at 5 dpf in 6 pools of 3 embryos per genotype. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3 M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was then removed without xxx the beads. Whilst still on the magnet  the beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalog number M0303L. 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Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3 M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was then removed without xxx the beads. Whilst still on the magnet  the beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalog number M0303L. 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