{"database": "metadata", "table": "run_metadata", "rows": [[11181, "ERR10180594", "ERX9716160", "ERS13447536", "ERP140949", "PRJEB56027", "Transcriptional coupling between Nrf2 and aB crystallin in the lens and heart of zebrafish under proteostatic stress", "E-MTAB-12172", "Transcriptome Analysis", "To identify the molecular pathways in the cryaba mutant by Nrf2 deficiency  we performed high throughput transcriptome profiling on adult zebrafish lens and heart tissues from the WT  cryaba /   nrf2fh318/fh318  and cryaba / ;nrf2fh318/fh318 lines.", "ENA FIRST PUBLIC:2022 09 24|ENA LAST UPDATE:2022 09 24", null, "Protocols: adult zebrafish lens tissues were dissected The following mutant and transgenic fish lines were used: cryabavu612 cry\uf061ba / ; cryabbvu613 cry\uf061bb / ; nrf2fh318. All animal procedures were approved by the Vanderbilt University Institutional Animal Care and Use Committee. Gupta  T. and M.C. Mullins  Dissection of organs from the adult zebrafish. J Vis Exp  201037. The following mutant and transgenic fish lines were used: cryabavu612 cry\uf061ba / ; cryabbvu613 cry\uf061bb / ; nrf2fh318. All animal procedures were approved by the Vanderbilt University Institutional Animal Care and Use Committee. RNA was extracted using a Trizol reagent with RNA clean up kit Zymo R2050 according to the manufacturer's instruction. Raw sequencing reads were obtained for the paired end samples.", "lens abaKO 1", "SAMEA111349989", "Vanderbilt Univiersity", "ENA FIRST PUBLIC:2022 09 24|ENA LAST UPDATE:2022 09 24|External Id:SAMEA111349989|INSDC center alias:Vanderbilt Univiersity|INSDC center name:Vanderbilt Univiersity|INSDC first public:2022 09 24T00:21:08Z|INSDC last update:2022 09 24T00:21:08Z|INSDC status:public|Submitter Id:E MTAB 12172:lens abaKO 1|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:cryaba knockout|organism part:lens|sample name:E MTAB 12172:lens abaKO 1|sex:male", null, null, null, null, null, null, null, null, "Illumina NovaSeq 6000 paired end sequencing; Transcriptional coupling between Nrf2 and aB crystallin in the lens and heart of zebrafish under proteostatic stress", "E MTAB 12172:lens abaKO 1 p", "lens abaKO 1 p", "Transcriptional coupling between Nrf2 and aB crystallin in the lens and heart of zebrafish under proteostatic stress", "adult zebrafish lens tissues were dissected The following mutant and transgenic fish lines were used: cryabavu612 cry\uf061ba / ; cryabbvu613 cry\uf061bb / ; nrf2fh318. All animal procedures were approved by the Vanderbilt University Institutional Animal Care and Use Committee. Gupta  T. and M.C. Mullins  Dissection of organs from the adult zebrafish. J Vis Exp  201037. The following mutant and transgenic fish lines were used: cryabavu612 cry\uf061ba / ; cryabbvu613 cry\uf061bb / ; nrf2fh318. All animal procedures were approved by the Vanderbilt University Institutional Animal Care and Use Committee. RNA was extracted using a Trizol reagent with RNA clean up kit Zymo R2050 according to the manufacturer's instruction.  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