{"database": "metadata", "table": "run_metadata", "rows": [[10376, "ERR8058373", "ERX7625094", "ERS10119354", "ERP134902", "PRJEB50332", "RNA seq pf zebrafish embryos treated with drugs of abuse  morphine and cocaine", "E-MTAB-11346", "Transcriptome Analysis", "To determine the transcriptomic changes elicted by drugs of abuse  zebrafish embryos were exposed to 10uM morphine from xxx hpf ttwo xxx hpf. Embryos were euthanized and deep frozen with N2l. RNA was extracted and an RNA seq assay was performed with Illumina Platform", "ENA FIRST PUBLIC:2023 01 03|ENA LAST UPDATE:2023 01 03", null, "Protocols: At 72 hpf embryos were sacrificed by cold exposure  incubating the multiwell plates on crushed ice. Embryos were transferred to eppendorfs  medium was removed and they were frozen in N2l. Tissue was stored at  80\u00baC until use. RNA was obtained using PureLinkTM RNA Mini Kit Ambion Fisher Scientific following the manufacturer\u00b4s instructions. In column DNase I digestion Invitrogen Fisher Scientific was performed to remove the traces of gDNA. RNA concentration was initially determined with a NanoDrop 2000C spectrophotometer Thermo Scientific  the purity of the sample was established according to the ratio of absorbances measured at 260/280 nm  and RNA integrity was check with a Bioanalyzer RNA Agilent. Ribosomal RNA was depleted using RiboMinus\u2122 RNA was fragmented  reverse transcrided using random hexamers  three prime and five prime adapters were attached and library was amplified by PCR", "Morphine 1", "SAMEA12512750", "Dept. Biochemistry and Molecular Biology. Faculty of Medicine. Instituto de Neurociencias de Castilla y Leon (INCyL). University of Salamanca, Spain.", "ENA FIRST PUBLIC:2022 12 31|ENA LAST UPDATE:2022 12 31|External Id:SAMEA12512750|INSDC center alias:Dept. Biochemistry and Molecular Biology. Faculty of Medicine. Instituto de Neurociencias de Castilla y Leon INCyL. University of Salamanca  Spain.|INSDC center name:Dept. Biochemistry and Molecular Biology. Faculty of Medicine. Instituto de Neurociencias de Castilla y Leon INCyL. University of Salamanca  Spain.|INSDC first public:2022 12 31T00:57:45Z|INSDC last update:2022 12 31T00:57:45Z|INSDC status:public|Submitter Id:E MTAB 11346:Morphine 1|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo|developmental stage:72|genotype:wild type genotype|isolate:not applicable|organism part:whole organism|sample name:E MTAB 11346:Morphine 1|strain:AB", null, null, null, null, null, null, null, null, "Illumina HiSeq 4000 paired end sequencing; RNA seq pf zebrafish embryos treated with drugs of abuse  morphine and cocaine", "E MTAB 11346:Morphine 1 p", "Morphine 1 p", "RNA seq pf zebrafish embryos treated with drugs of abuse  morphine and cocaine", "At 72 hpf embryos were sacrificed by cold exposure  incubating the multiwell plates on crushed ice. 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