{"database": "metadata", "table": "run_metadata", "rows": [[10241, "ERR7131165", "ERX6698606", "ERS8070395", "ERP132560", "PRJEB48218", "Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb", "E-MTAB-11083", "Transcriptome Analysis", "To investigate the effects of rabies infection on neuronal gene expression  we compared gene profiles of rabies infected and non infected GABAergic neurons in the Zebrafish olfactory bulb.", "ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07", null, "Protocols: EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.", "F8 mCherry", "SAMEA10418611", "Friedrich Miescher Institute for Biomedical Research", "ENA first public:2021 12 01|ENA last update:2021 12 01|External Id:SAMEA10418611|INSDC center alias:Friedrich Miescher Institute for Biomedical Research|INSDC center name:Friedrich Miescher Institute for Biomedical Research|INSDC first public:2021 12 01T00:23:59Z|INSDC last update:2021 12 01T00:23:59Z|INSDC status:public|Submitter Id:E MTAB 11083:F8 mCherry|age:9|broker name:ArrayExpress|cell type:GABAergic neuron|common name:zebrafish|developmental stage:adult|fraction:mCherry+/GFP |genotype:Tg[gad1b:Gal4  UAS:TVA mCherry]|individual:pool 8|organism part:olfactory bulb|sample name:E MTAB 11083:F8 mCherry|sex:mixed|stimulus:injected with EnvA RV GFP|strain:Ab x Tu x TL x WIK", null, null, null, null, null, null, null, null, "Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb", "E MTAB 11083:F8 mCherry s", "F8 mCherry s", "Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb", "EnvA RV GFP was injected to olfactory bulb in Tg[gad1b:Gal4  UAS:TVA mCherry] fish. Fish were kept in the standard fish system at 36 degree for 3 4 days. postwards  olfactory bulbs were extracted from 3 5 fish and the samples were pooled. post standard dissociation processes  cells were sorted by their fluorescent markers using LSRII. Batch 1 pool 1 to 3 was done 7 days before batch 2 pool 4 to 8. RNA was purified using single cell RNA purification Kit Norgen  cat. 51800 mRNA seq libraries were generated using the SmartSeq2 approach Picelli et al  Nature protocol 2014  with the following modifications: For cDNA pre amplification  up to 10ng of RNA was used as input typically 1 3ng  and Reverse Transcription was performed using Superscript IV Thermo Fisher Scientific   50C for 10min  80C for 10min. Amplified cDNA 1ng were converted to indexed sequencing libraries by tagmentation  using in house purified Tn5 Picelli et al  Genome Research 2014 and Illumina Nextera primers.", "Experimental Factor: fraction:mCherry+/GFP ", "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "SINGLE", "ILLUMINA", "Illumina HiSeq 2500", null, "ERP132560", "Illumina HiSeq 2500 sequencing; Effect of rabies virus infection on gene expression in GABAergic neurons in the Zebrafish olfactory bulb", "ENA FIRST PUBLIC:2022 07 07|ENA LAST UPDATE:2022 07 07", "2874F23-1_210715_D00404_0538_BCD91CANXX_TCGACGTC-CGTCTAAT_L006_R1_001.fastq.gz", "fastq", 667760646.0, 13093346.0, "E MTAB 11083:2874F23 1 210715 D00404 0538 BCD91CANXX TCGACGTC CGTCTAAT L006", "0:51 1:0", "A:179948739;C:150541918;G:145038845;T:192184092;N:47052", 51, 0, null, null, 179948739, 150541918, 145038845, 192184092, 47052, "ERX6698606", "ERS8070395", "ERA6757553", "Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive", "Friedrich Miescher Institute for Biomedical Research|European Nucleotide Archive", 1, 0.71661, null, 0.19774, null, 0.74576, null, 0.52117, null, 51, null, "B", null, "usable mapping rate", "illumina", "hiseq_era", "unknown", "poly_a", "nextera", "sc", "single_cell_plate", "smartseq", null, "Switzerland", "2021-12-01", "Adult", "Adult", "Brain", "Nervous System"]], "columns": ["rowid", "run.accession", "experiment.accession", "sample.accession", "study.accession", "bioproject", "study.title", "study.alias", "study.type", "study.abstract", "study.attributes", "study.PMIDs", "sample.description", "sample.title", "sample.alias", "sample.centername", "sample.attributes", "GEOsample.title", "GEOsample.dataprocessing", "GEOsample.source", "GEOsample.treatmentprotocol", "GEOsample.extractprotocol", "GEOsample.growthprotocol", "GEOsample.characteristics", "GEOsample.accession", "experiment.title", "experiment.alias", "experiment.library_name", "experiment.design_description", "experiment.library_construction_protocol", "experiment.attributes", "experiment.library_strategy", "experiment.library_source", "experiment.library_selection", "experiment.library_layout", "experiment.platform", "experiment.instrument_model", "experiment.spot_descriptor", "experiment.study_ref", "run.title", "run.attributes", "run.filename", "run.semantic_name", "run.total_bases", "run.total_spots", "run.alias", "run.read_lengths", "run.base_counts", "run.r1_length", "run.r2_length", "run.r3_length", "run.r4_length", "run.Acount", "run.Ccount", "run.Gcount", "run.Tcount", "run.Ncount", "run.experiment", "run.pool_member", "submission.accession", "submission.srasource", "submission.bioprojectsource", "seqdetective.n_mates", "seqdetective.mapping_rate.mate1", "seqdetective.mapping_rate.mate2", "seqdetective.nofeature_rate.mate1", "seqdetective.nofeature_rate.mate2", "seqdetective.sparsity.mate1", "seqdetective.sparsity.mate2", "seqdetective.pos_strand_rate.mate1", "seqdetective.pos_strand_rate.mate2", "seqdetective.readlen.mate1", "seqdetective.readlen.mate2", "seqdetective.judgement.mate1", "seqdetective.judgement.mate2", "seqdetective.judgement.reason", "platform_family", "instrument_generation", "read_bias", "selection_class", "prep_kit", "sc_or_bulk", "tech_class", "technology", "tech_variant", "submission.bioprojectsource.country", "earliest_date", "devstage_curation", "devstage_curation_coarse", "tissue_curation", "tissue_curation_coarse"], "primary_keys": ["rowid"], "primary_key_values": ["10241"], "units": {}, "query_ms": 10.846458000742132}