{"database": "metadata", "table": "run_metadata", "is_view": false, "human_description_en": "where technology = \"unknown\", tissue_curation = \"Head\" and tissue_curation_coarse = \"Nervous System\"", "rows": [[7950, "ERR015567", "ERX005930", "ERS000089", "ERP000263", "PRJEB2208", "Zebrafish gene three prime end pull down for genome annotation", "E-MTAB-308", "Transcriptome Analysis", null, null, null, "RNA extracted from male adult zebrafish head", null, "SAMEA708835", "SC", "ENA FIRST PUBLIC:2010 02 26T10:44:13Z|ENA LAST UPDATE:2018 03 08T15:51:30Z|External Id:SAMEA708835|INSDC center name:SC|INSDC first public:2010 02 26T10:44:13Z|INSDC last update:2018 03 08T15:51:30Z|INSDC status:public|Submitter Id:ZF male head sample1|common name:zebrafish|sample name:ZF male head sample1|scientific name:Danio rerio|sex:male|strain:Tuebingen", null, null, null, null, null, null, null, null, "Illumina Genome Analyzer II paired end sequencing; Zebrafish gene 3 prime end pull down for genome annotation", "E MTAB 308:Illumina Genome Analyzer II sequencing of adult Zebrafish male head dpf three prime pull down paired end 250 to 300 bp insert", "Zebrafish adult male head mRNA three prime end", "Zebrafish gene three prime end pull down for genome annotation", "20 ug of total RNA was fragmented using RNA Fragmentation Reagent Ambion for 5 minutes at 70 C and ethanol precipitated with glycogen and LiCl.   RNA was annealed to the oligo stBPM1polyT22 biotin GGCCAGTCCTGGAGTTTTTTTTTTTTTTTTTTTTTTVN and bound to streptavidin  magnetic beads.   post washing by pull down on a magnet  the bound RNA was reverse transcribed with SuperScript II Invitrogen and a second strand synthesised with DNA polymerase I Promega and RNase H NEB.   post further washing  the double strand cDNA was released from the beads with BpmI NEB.   The cDNA was recovered with the QIAgen PCR Purification Kit and made into a standard Illumina library following the manufacturer's protocol with a fragment size of 250 to 300 bp.", "Experimental Factor: DEVELOPMENTAL STAGE:adult|Experimental Factor: ORGANISM PART:head|Experimental Factor: SEX:male", "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "Illumina Genome Analyzer II", null, "ERP000263", "Illumina Genome Analyzer II paired end sequencing; Zebrafish gene three prime end pull down for genome annotation", "ENA FIRST PUBLIC:2010 08 19|ENA LAST UPDATE:2018 11 16", "3444_7.srf", "srf", 1384012896.0, 9105348.0, "E MTAB 308:Illumina Genome Analyzer II sequencing of adult Zebrafish male head dpf three prime pull down paired end 250 to 300 bp insert", "0:76 1:76", "A:369912216;C:306963547;G:292249178;T:392053840;N:22834115", 76, 76, null, null, 369912216, 306963547, 292249178, 392053840, 22834115, "ERX005930", "ERS000089", "ERA010603", "SC|Wellcome Trust Sanger Institute", "SC|Wellcome Trust Sanger Institute", 2, 0.95932, 0.96084, 0.20719, 0.21321, 0.73959, 0.74763, 0.47227, 0.48033, 76, 76, "B", "B", "biological fallback assumption", "illumina", "early_illumina", "3prime", "other", "unknown", "bulk", "unknown", "unknown", null, "United Kingdom", "2010-02-26", "Adult", "Adult", "Head", "Nervous System"], [8059, "ERR022480", "ERX008923", "ERS000088", "ERP000400", "PRJEB2333", "Sequencing the Zebrafish transcriptome form a range of tissues and developmental stages using the Illumina Genome Analyzer", "E-MTAB-434", "Other", null, null, null, null, "ZF female head sample1", "SAMEA708836", "Wellcome Sanger Institute", "Alias:ZF female head sample1|Description:RNA extracted from female adult zebrafish head|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2010 02 26T10:44:13Z|INSDC last update:2018 03 08T15:24:37Z|INSDC status:public|SRA accession:ERS000088|Sample Name:ERS000088|Sex:female|Strain:Tuebingen|Title:Danio rerio", null, null, null, null, null, null, null, null, "Illumina Genome Analyzer II paired end sequencing; Sequencing the Zebrafish transcriptome form a range of tissues and developmental stages using the Illumina Genome Analyzer", "E MTAB 434:sequencing of Zebrafish adult female head", "RNA from Zebrafish adult female head", "Sequencing the Zebrafish transcriptome form a range of tissues and developmental stages using the Illumina Genome Analyzer", "Zebrafish embyos or tissues were collected from a Tuebingen strain incross and grown at 28 C.   Collected samples were snap frozen on dry ice and stored at  70 C. Total RNA was extracted using Trizol Reagent Invitrogen following the manufacturer's instructions. Pellets were resuspended RNase free 10 mM Tris pH 7.5 and the RNA was quantified using a NanoDrop ND 1000 Spectrophotometer Axon Instruments. Total RNA was made into an RNAseq Illumina library following the manufacturer's protocol including a DNase treatment between to 2 rounds of polyA pull down. The libraries have fragment size of 150 to 200 bp.", "Experimental Factor: DEVELPOMENTAL STAGE:adult|Experimental Factor: ORGANISM PART:head", "FL-cDNA", "TRANSCRIPTOMIC", "unspecified", "PAIRED", "ILLUMINA", "Illumina Genome Analyzer II", null, "ERP000400", "Illumina Genome Analyzer II paired end sequencing; Sequencing the Zebrafish transcriptome form a range of tissues and developmental stages using the Illumina Genome Analyzer", "ENA FIRST PUBLIC:2011 03 10|ENA LAST UPDATE:2018 11 16", "2719_5.srf", "srf", 1622590272.0, 15023984.0, "E MTAB 434:2719 5.srf", "0:54 1:54", "A:426435544;C:373743071;G:387589492;T:432086510;N:2735655", 54, 54, null, null, 426435544, 373743071, 387589492, 432086510, 2735655, "ERX008923", "ERS000088", "ERA015179", "SC|Wellcome Trust Sanger Institute", "SC|Wellcome Trust Sanger Institute", 2, 0.94624, 0.94338, 0.17949, 0.17965, 0.66454, 0.66872, 0.51097, 0.51377, 54, 54, "B", "B", "biological fallback assumption", "illumina", "early_illumina", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "United Kingdom", "2010-02-26", "Adult", "Adult", "Head", "Nervous System"], [8060, "ERR022481", "ERX008923", "ERS000088", "ERP000400", "PRJEB2333", "Sequencing the Zebrafish transcriptome form a range of tissues and developmental stages using the Illumina Genome Analyzer", "E-MTAB-434", "Other", null, null, null, null, "ZF female head sample1", "SAMEA708836", "Wellcome Sanger Institute", "Alias:ZF female head sample1|Description:RNA extracted from female adult zebrafish head|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2010 02 26T10:44:13Z|INSDC last update:2018 03 08T15:24:37Z|INSDC status:public|SRA accession:ERS000088|Sample Name:ERS000088|Sex:female|Strain:Tuebingen|Title:Danio rerio", null, null, null, null, null, null, null, null, "Illumina Genome Analyzer II paired end sequencing; Sequencing the Zebrafish transcriptome form a range of tissues and developmental stages using the Illumina Genome Analyzer", "E MTAB 434:sequencing of Zebrafish adult female head", "RNA from Zebrafish adult female head", "Sequencing the Zebrafish transcriptome form a range of tissues and developmental stages using the Illumina Genome Analyzer", "Zebrafish embyos or tissues were collected from a Tuebingen strain incross and grown at 28 C.   Collected samples were snap frozen on dry ice and stored at  70 C. Total RNA was extracted using Trizol Reagent Invitrogen following the manufacturer's instructions. Pellets were resuspended RNase free 10 mM Tris pH 7.5 and the RNA was quantified using a NanoDrop ND 1000 Spectrophotometer Axon Instruments. Total RNA was made into an RNAseq Illumina library following the manufacturer's protocol including a DNase treatment between to 2 rounds of polyA pull down. The libraries have fragment size of 150 to 200 bp.", "Experimental Factor: DEVELPOMENTAL STAGE:adult|Experimental Factor: ORGANISM PART:head", "FL-cDNA", "TRANSCRIPTOMIC", "unspecified", "PAIRED", "ILLUMINA", "Illumina Genome Analyzer II", null, "ERP000400", "Illumina Genome Analyzer II paired end sequencing; Sequencing the Zebrafish transcriptome form a range of tissues and developmental stages using the Illumina Genome Analyzer", "ENA FIRST PUBLIC:2011 03 10|ENA LAST UPDATE:2018 11 16", "2719_6.srf", "srf", 1693460736.0, 15680192.0, "E MTAB 434:2719 6.srf", "0:54 1:54", "A:445666325;C:389360280;G:403789644;T:451529226;N:3115261", 54, 54, null, null, 445666325, 389360280, 403789644, 451529226, 3115261, "ERX008923", "ERS000088", "ERA015179", "SC|Wellcome Trust Sanger Institute", "SC|Wellcome Trust Sanger Institute", 2, 0.94579, 0.94429, 0.17895, 0.17923, 0.66864, 0.67164, 0.51788, 0.51083, 54, 54, "B", "B", "biological fallback assumption", "illumina", "early_illumina", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "United Kingdom", "2010-02-26", "Adult", "Adult", "Head", "Nervous System"], [10407, "ERR9077202", "ERX8620336", "ERS10857224", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aWT5S8", "SAMEA13254181", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254181|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT5S8|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT5S8|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aWT5S8 s", "Sv2aWT5S8 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a+/+", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aWT5S8L001-1.fastq.gz", "fastq", 652046291.0, 8633105.0, "E MTAB 11505:sv2aWT5S8L001", "0:75.53 1:0", "A:159570243;C:156420495;G:154853315;T:181073030;N:129208", 75, 0, null, null, 159570243, 156420495, 154853315, 181073030, 129208, "ERX8620336", "ERS10857224", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.95245, null, 0.1148, null, 0.65756, null, 0.47011, null, 75, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10408, "ERR9077203", "ERX8620336", "ERS10857224", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aWT5S8", "SAMEA13254181", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254181|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT5S8|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT5S8|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aWT5S8 s", "Sv2aWT5S8 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a+/+", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aWT5S8L002-1.fastq.gz", "fastq", 653840525.0, 8656886.0, "E MTAB 11505:sv2aWT5S8L002", "0:75.53 1:0", "A:159902458;C:156826842;G:155509070;T:181475104;N:127051", 75, 0, null, null, 159902458, 156826842, 155509070, 181475104, 127051, "ERX8620336", "ERS10857224", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.95226, null, 0.1156, null, 0.65792, null, 0.4667, null, 76, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10409, "ERR9077204", "ERX8620336", "ERS10857224", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aWT5S8", "SAMEA13254181", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254181|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT5S8|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT5S8|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aWT5S8 s", "Sv2aWT5S8 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a+/+", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aWT5S8L003-1.fastq.gz", "fastq", 659584196.0, 8732885.0, "E MTAB 11505:sv2aWT5S8L003", "0:75.53 1:0", "A:161257146;C:158299734;G:156793443;T:183128972;N:104901", 75, 0, null, null, 161257146, 158299734, 156793443, 183128972, 104901, "ERX8620336", "ERS10857224", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.95298, null, 0.11485, null, 0.65798, null, 0.46853, null, 76, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10410, "ERR9077205", "ERX8620336", "ERS10857224", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aWT5S8", "SAMEA13254181", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254181|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT5S8|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT5S8|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aWT5S8 s", "Sv2aWT5S8 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  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Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aWT4S7", "SAMEA13254180", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254180|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT4S7|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT4S7|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aWT4S7 s", "Sv2aWT4S7 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a+/+", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aWT4S7L001-1.fastq.gz", "fastq", 683751258.0, 9051795.0, "E MTAB 11505:sv2aWT4S7L001", "0:75.54 1:0", "A:162889923;C:167127644;G:164779384;T:188822907;N:131400", 75, 0, null, null, 162889923, 167127644, 164779384, 188822907, 131400, "ERX8620335", "ERS10857223", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.95739, null, 0.0959, null, 0.6606, null, 0.46826, null, 75, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10412, "ERR9077199", "ERX8620335", "ERS10857223", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aWT4S7", "SAMEA13254180", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254180|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT4S7|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT4S7|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aWT4S7 s", "Sv2aWT4S7 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a+/+", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aWT4S7L002-1.fastq.gz", "fastq", 684187711.0, 9057591.0, "E MTAB 11505:sv2aWT4S7L002", "0:75.54 1:0", "A:162923879;C:167246213;G:165071145;T:188818774;N:127700", 75, 0, null, null, 162923879, 167246213, 165071145, 188818774, 127700, "ERX8620335", "ERS10857223", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.95724, null, 0.0959, null, 0.66036, null, 0.47026, null, 76, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10413, "ERR9077200", "ERX8620335", "ERS10857223", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aWT4S7", "SAMEA13254180", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254180|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT4S7|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT4S7|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aWT4S7 s", "Sv2aWT4S7 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a+/+", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aWT4S7L003-1.fastq.gz", "fastq", 692320213.0, 9165132.0, "E MTAB 11505:sv2aWT4S7L003", "0:75.54 1:0", "A:164772792;C:169329920;G:166952052;T:191164422;N:101027", 75, 0, null, null, 164772792, 169329920, 166952052, 191164422, 101027, "ERX8620335", "ERS10857223", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.95786, null, 0.09701, null, 0.66131, null, 0.47564, null, 76, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10414, "ERR9077201", "ERX8620335", "ERS10857223", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aWT4S7", "SAMEA13254180", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254180|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT4S7|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT4S7|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aWT4S7 s", "Sv2aWT4S7 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a+/+", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aWT4S7L004-1.fastq.gz", "fastq", 697047881.0, 9227702.0, "E MTAB 11505:sv2aWT4S7L004", "0:75.54 1:0", "A:165881728;C:170388393;G:168105900;T:192572068;N:99792", 75, 0, null, null, 165881728, 170388393, 168105900, 192572068, 99792, "ERX8620335", "ERS10857223", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.9584, null, 0.09565, null, 0.66115, null, 0.46967, null, 76, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10415, "ERR9077194", "ERX8620334", "ERS10857222", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aWT3S2", "SAMEA13254179", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254179|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT3S2|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT3S2|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aWT3S2 s", "Sv2aWT3S2 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a+/+", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aWT3S2L001-1.fastq.gz", "fastq", 723508205.0, 9579193.0, "E MTAB 11505:sv2aWT3S2L001", "0:75.53 1:0", "A:177832489;C:174193135;G:169978598;T:201360184;N:143799", 75, 0, null, null, 177832489, 174193135, 169978598, 201360184, 143799, "ERX8620334", "ERS10857222", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.95218, null, 0.11732, null, 0.65916, null, 0.47955, null, 71, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10416, "ERR9077195", "ERX8620334", "ERS10857222", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aWT3S2", "SAMEA13254179", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254179|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT3S2|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT3S2|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aWT3S2 s", "Sv2aWT3S2 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a+/+", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aWT3S2L002-1.fastq.gz", "fastq", 726190024.0, 9614674.0, "E MTAB 11505:sv2aWT3S2L002", "0:75.53 1:0", "A:178408224;C:174776263;G:170820745;T:202047224;N:137568", 75, 0, null, null, 178408224, 174776263, 170820745, 202047224, 137568, "ERX8620334", "ERS10857222", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.95093, null, 0.11567, null, 0.65865, null, 0.46877, null, 75, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10417, "ERR9077196", "ERX8620334", "ERS10857222", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aWT3S2", "SAMEA13254179", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254179|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT3S2|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT3S2|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aWT3S2 s", "Sv2aWT3S2 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a+/+", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aWT3S2L003-1.fastq.gz", "fastq", 735499955.0, 9737845.0, "E MTAB 11505:sv2aWT3S2L003", "0:75.53 1:0", "A:180659962;C:177141362;G:172905323;T:204678308;N:115000", 75, 0, null, null, 180659962, 177141362, 172905323, 204678308, 115000, "ERX8620334", "ERS10857222", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.95233, null, 0.11614, null, 0.65898, null, 0.47114, null, 76, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10418, "ERR9077197", "ERX8620334", "ERS10857222", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aWT3S2", "SAMEA13254179", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254179|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT3S2|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT3S2|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aWT3S2 s", "Sv2aWT3S2 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a+/+", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aWT3S2L004-1.fastq.gz", "fastq", 745285512.0, 9867337.0, "E MTAB 11505:sv2aWT3S2L004", "0:75.53 1:0", "A:183067487;C:179408382;G:175250814;T:207445912;N:112917", 75, 0, null, null, 183067487, 179408382, 175250814, 207445912, 112917, "ERX8620334", "ERS10857222", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.95245, null, 0.11655, null, 0.65857, null, 0.47087, null, 76, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10419, "ERR9077190", "ERX8620333", "ERS10857221", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aWT1S1", "SAMEA13254178", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254178|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT1S1|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT1S1|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aWT1S1 s", "Sv2aWT1S1 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a+/+", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aWT1S1L001-1.fastq.gz", "fastq", 647414748.0, 8571883.0, "E MTAB 11505:sv2aWT1S1L001", "0:75.53 1:0", "A:154593859;C:160498057;G:153809612;T:178389112;N:124108", 75, 0, null, null, 154593859, 160498057, 153809612, 178389112, 124108, "ERX8620333", "ERS10857221", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.95955, null, 0.08686, null, 0.65955, null, 0.47406, null, 76, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10420, "ERR9077191", "ERX8620333", "ERS10857221", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aWT1S1", "SAMEA13254178", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254178|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT1S1|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT1S1|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aWT1S1 s", "Sv2aWT1S1 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a+/+", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aWT1S1L002-1.fastq.gz", "fastq", 646657758.0, 8561861.0, "E MTAB 11505:sv2aWT1S1L002", "0:75.53 1:0", "A:154348044;C:160294625;G:153804048;T:178092212;N:118829", 75, 0, null, null, 154348044, 160294625, 153804048, 178092212, 118829, "ERX8620333", "ERS10857221", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.95942, null, 0.08632, null, 0.65991, null, 0.47539, null, 76, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10421, "ERR9077192", "ERX8620333", "ERS10857221", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aWT1S1", "SAMEA13254178", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254178|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT1S1|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT1S1|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aWT1S1 s", "Sv2aWT1S1 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a+/+", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aWT1S1L003-1.fastq.gz", "fastq", 654781451.0, 8669333.0, "E MTAB 11505:sv2aWT1S1L003", "0:75.53 1:0", "A:156252744;C:162396721;G:155681450;T:180352911;N:97625", 75, 0, null, null, 156252744, 162396721, 155681450, 180352911, 97625, "ERX8620333", "ERS10857221", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.95972, null, 0.08498, null, 0.66137, null, 0.47457, null, 75, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10422, "ERR9077193", "ERX8620333", "ERS10857221", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aWT1S1", "SAMEA13254178", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254178|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT1S1|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT1S1|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aWT1S1 s", "Sv2aWT1S1 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a+/+", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aWT1S1L004-1.fastq.gz", "fastq", 659716937.0, 8734618.0, "E MTAB 11505:sv2aWT1S1L004", "0:75.53 1:0", "A:157367211;C:163585842;G:156911139;T:181757809;N:94936", 75, 0, null, null, 157367211, 163585842, 156911139, 181757809, 94936, "ERX8620333", "ERS10857221", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.95985, null, 0.08585, null, 0.65857, null, 0.47349, null, 76, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10423, "ERR9077186", "ERX8620332", "ERS10857220", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aHO6S12", "SAMEA13254177", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254177|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO6S12|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO6S12|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aHO6S12 s", "Sv2aHO6S12 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a / ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aHO6S12L001-1.fastq.gz", "fastq", 675018261.0, 8937958.0, "E MTAB 11505:sv2aHO6S12L001", "0:75.52 1:0", "A:166564263;C:160539328;G:160003980;T:187773509;N:137181", 75, 0, null, null, 166564263, 160539328, 160003980, 187773509, 137181, "ERX8620332", "ERS10857220", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.94964, null, 0.13009, null, 0.65413, null, 0.47079, null, 76, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10424, "ERR9077187", "ERX8620332", "ERS10857220", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aHO6S12", "SAMEA13254177", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254177|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO6S12|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO6S12|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aHO6S12 s", "Sv2aHO6S12 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a / ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aHO6S12L002-1.fastq.gz", "fastq", 672995667.0, 8911161.0, "E MTAB 11505:sv2aHO6S12L002", "0:75.52 1:0", "A:165990319;C:160039398;G:159722501;T:187108781;N:134668", 75, 0, null, null, 165990319, 160039398, 159722501, 187108781, 134668, "ERX8620332", "ERS10857220", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.94837, null, 0.13029, null, 0.65744, null, 0.47959, null, 76, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10425, "ERR9077188", "ERX8620332", "ERS10857220", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aHO6S12", "SAMEA13254177", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254177|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO6S12|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO6S12|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aHO6S12 s", "Sv2aHO6S12 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a / ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aHO6S12L003-1.fastq.gz", "fastq", 683276767.0, 9047147.0, "E MTAB 11505:sv2aHO6S12L003", "0:75.52 1:0", "A:168501690;C:162583852;G:162063190;T:190018001;N:110034", 75, 0, null, null, 168501690, 162583852, 162063190, 190018001, 110034, "ERX8620332", "ERS10857220", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.94983, null, 0.13248, null, 0.6565, null, 0.47977, null, 76, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10426, "ERR9077189", "ERX8620332", "ERS10857220", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aHO6S12", "SAMEA13254177", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254177|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO6S12|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO6S12|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aHO6S12 s", "Sv2aHO6S12 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a / ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aHO6S12L004-1.fastq.gz", "fastq", 686469910.0, 9089431.0, "E MTAB 11505:sv2aHO6S12L004", "0:75.52 1:0", "A:169287189;C:163276361;G:162844724;T:190954218;N:107418", 75, 0, null, null, 169287189, 163276361, 162844724, 190954218, 107418, "ERX8620332", "ERS10857220", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.95061, null, 0.13029, null, 0.65389, null, 0.48044, null, 76, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10427, "ERR9077182", "ERX8620331", "ERS10857219", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aHO4S10", "SAMEA13254176", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254176|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO4S10|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO4S10|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aHO4S10 s", "Sv2aHO4S10 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a / ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aHO4S10L001-1.fastq.gz", "fastq", 713858966.0, 9452474.0, "E MTAB 11505:sv2aHO4S10L001", "0:75.52 1:0", "A:177913695;C:169425317;G:167540012;T:198835532;N:144410", 75, 0, null, null, 177913695, 169425317, 167540012, 198835532, 144410, "ERX8620331", "ERS10857219", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.94848, null, 0.12981, null, 0.6636, null, 0.47772, null, 76, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10428, "ERR9077183", "ERX8620331", "ERS10857219", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aHO4S10", "SAMEA13254176", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254176|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO4S10|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO4S10|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aHO4S10 s", "Sv2aHO4S10 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a / ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aHO4S10L002-1.fastq.gz", "fastq", 717619593.0, 9502301.0, "E MTAB 11505:sv2aHO4S10L002", "0:75.52 1:0", "A:178830542;C:170284720;G:168594514;T:199773382;N:136435", 75, 0, null, null, 178830542, 170284720, 168594514, 199773382, 136435, "ERX8620331", "ERS10857219", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.94812, null, 0.12868, null, 0.6645, null, 0.47281, null, 75, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10429, "ERR9077184", "ERX8620331", "ERS10857219", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aHO4S10", "SAMEA13254176", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254176|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO4S10|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO4S10|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aHO4S10 s", "Sv2aHO4S10 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a / ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aHO4S10L003-1.fastq.gz", "fastq", 727263626.0, 9629858.0, "E MTAB 11505:sv2aHO4S10L003", "0:75.52 1:0", "A:181201278;C:172619520;G:170780953;T:202550397;N:111478", 75, 0, null, null, 181201278, 172619520, 170780953, 202550397, 111478, "ERX8620331", "ERS10857219", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.94768, null, 0.12827, null, 0.66596, null, 0.47332, null, 76, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10430, "ERR9077185", "ERX8620331", "ERS10857219", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aHO4S10", "SAMEA13254176", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254176|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO4S10|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO4S10|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aHO4S10 s", "Sv2aHO4S10 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a / ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aHO4S10L004-1.fastq.gz", "fastq", 736595278.0, 9753337.0, "E MTAB 11505:sv2aHO4S10L004", "0:75.52 1:0", "A:183510872;C:174806534;G:173027794;T:205140640;N:109438", 75, 0, null, null, 183510872, 174806534, 173027794, 205140640, 109438, "ERX8620331", "ERS10857219", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.94925, null, 0.12904, null, 0.66417, null, 0.47297, null, 76, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10431, "ERR9077178", "ERX8620330", "ERS10857218", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aHO3S6", "SAMEA13254175", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254175|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO3S6|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO3S6|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aHO3S6 s", "Sv2aHO3S6 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a / ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aHO3S6L001-1.fastq.gz", "fastq", 710756305.0, 9410862.0, "E MTAB 11505:sv2aHO3S6L001", "0:75.53 1:0", "A:175585613;C:170261120;G:166658917;T:198106388;N:144267", 75, 0, null, null, 175585613, 170261120, 166658917, 198106388, 144267, "ERX8620330", "ERS10857218", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.94827, null, 0.14002, null, 0.66785, null, 0.474, null, 75, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10432, "ERR9077179", "ERX8620330", "ERS10857218", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aHO3S6", "SAMEA13254175", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254175|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO3S6|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO3S6|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aHO3S6 s", "Sv2aHO3S6 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a / ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aHO3S6L002-1.fastq.gz", "fastq", 715330324.0, 9471415.0, "E MTAB 11505:sv2aHO3S6L002", "0:75.53 1:0", "A:176679775;C:171311031;G:167903237;T:199299483;N:136798", 75, 0, null, null, 176679775, 171311031, 167903237, 199299483, 136798, "ERX8620330", "ERS10857218", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.9469, null, 0.13915, null, 0.67004, null, 0.47654, null, 75, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10433, "ERR9077180", "ERX8620330", "ERS10857218", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aHO3S6", "SAMEA13254175", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254175|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO3S6|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO3S6|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aHO3S6 s", "Sv2aHO3S6 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a / ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aHO3S6L003-1.fastq.gz", "fastq", 722810562.0, 9570364.0, "E MTAB 11505:sv2aHO3S6L003", "0:75.53 1:0", "A:178442845;C:173192786;G:169595085;T:201465522;N:114324", 75, 0, null, null, 178442845, 173192786, 169595085, 201465522, 114324, "ERX8620330", "ERS10857218", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.94918, null, 0.13979, null, 0.66902, null, 0.47572, null, 75, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10434, "ERR9077181", "ERX8620330", "ERS10857218", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aHO3S6", "SAMEA13254175", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254175|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO3S6|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO3S6|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aHO3S6 s", "Sv2aHO3S6 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a / ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aHO3S6L004-1.fastq.gz", "fastq", 732990507.0, 9705054.0, "E MTAB 11505:sv2aHO3S6L004", "0:75.53 1:0", "A:180951130;C:175567778;G:172037542;T:204323488;N:110569", 75, 0, null, null, 180951130, 175567778, 172037542, 204323488, 110569, "ERX8620330", "ERS10857218", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.94812, null, 0.13893, null, 0.67085, null, 0.47502, null, 75, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10435, "ERR9077174", "ERX8620329", "ERS10857217", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aHET7S9", "SAMEA13254174", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254174|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET7S9|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET7S9|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aHET7S9 s", "Sv2aHET7S9 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a+/ ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aHET7S9L001-1.fastq.gz", "fastq", 773209432.0, 10238087.0, "E MTAB 11505:sv2aHET7S9L001", "0:75.52 1:0", "A:191962953;C:184699376;G:180774008;T:215620759;N:152336", 75, 0, null, null, 191962953, 184699376, 180774008, 215620759, 152336, "ERX8620329", "ERS10857217", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.95047, null, 0.12485, null, 0.65705, null, 0.4775, null, 76, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10436, "ERR9077175", "ERX8620329", "ERS10857217", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aHET7S9", "SAMEA13254174", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254174|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET7S9|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET7S9|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aHET7S9 s", "Sv2aHET7S9 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a+/ ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aHET7S9L002-1.fastq.gz", "fastq", 773106152.0, 10236677.0, "E MTAB 11505:sv2aHET7S9L002", "0:75.52 1:0", "A:191812833;C:184676419;G:180997547;T:215473732;N:145621", 75, 0, null, null, 191812833, 184676419, 180997547, 215473732, 145621, "ERX8620329", "ERS10857217", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.95016, null, 0.12464, null, 0.65482, null, 0.47419, null, 76, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10437, "ERR9077176", "ERX8620329", "ERS10857217", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aHET7S9", "SAMEA13254174", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254174|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET7S9|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET7S9|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aHET7S9 s", "Sv2aHET7S9 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a+/ ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aHET7S9L003-1.fastq.gz", "fastq", 783061051.0, 10368445.0, "E MTAB 11505:sv2aHET7S9L003", "0:75.52 1:0", "A:194289152;C:187108364;G:183177036;T:218370500;N:115999", 75, 0, null, null, 194289152, 187108364, 183177036, 218370500, 115999, "ERX8620329", "ERS10857217", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.95172, null, 0.12488, null, 0.65445, null, 0.48157, null, 76, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10438, "ERR9077177", "ERX8620329", "ERS10857217", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aHET7S9", "SAMEA13254174", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254174|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET7S9|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET7S9|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aHET7S9 s", "Sv2aHET7S9 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a+/ ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aHET7S9L004-1.fastq.gz", "fastq", 790150025.0, 10462214.0, "E MTAB 11505:sv2aHET7S9L004", "0:75.52 1:0", "A:196056500;C:188746833;G:184927588;T:220305922;N:113182", 75, 0, null, null, 196056500, 188746833, 184927588, 220305922, 113182, "ERX8620329", "ERS10857217", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.95285, null, 0.12478, null, 0.65551, null, 0.4763, null, 76, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10439, "ERR9077170", "ERX8620328", "ERS10857216", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aHET4S5", "SAMEA13254173", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254173|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET4S5|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET4S5|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aHET4S5 s", "Sv2aHET4S5 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  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Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aHET4S5", "SAMEA13254173", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254173|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET4S5|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET4S5|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aHET4S5 s", "Sv2aHET4S5 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a+/ ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aHET4S5L002-1.fastq.gz", "fastq", 684402241.0, 9059974.0, "E MTAB 11505:sv2aHET4S5L002", "0:75.54 1:0", "A:161493428;C:167520248;G:166882054;T:188383816;N:122695", 75, 0, null, null, 161493428, 167520248, 166882054, 188383816, 122695, "ERX8620328", "ERS10857216", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.95537, null, 0.09915, null, 0.65719, null, 0.48261, null, 76, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10441, "ERR9077172", "ERX8620328", "ERS10857216", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aHET4S5", "SAMEA13254173", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254173|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET4S5|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET4S5|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aHET4S5 s", "Sv2aHET4S5 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a+/ ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aHET4S5L003-1.fastq.gz", "fastq", 694099126.0, 9188245.0, "E MTAB 11505:sv2aHET4S5L003", "0:75.54 1:0", "A:163727378;C:169989210;G:169143224;T:191137588;N:101726", 75, 0, null, null, 163727378, 169989210, 169143224, 191137588, 101726, "ERX8620328", "ERS10857216", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.95629, null, 0.09861, null, 0.65705, null, 0.47187, null, 75, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10442, "ERR9077173", "ERX8620328", "ERS10857216", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aHET4S5", "SAMEA13254173", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254173|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET4S5|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET4S5|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aHET4S5 s", "Sv2aHET4S5 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a+/ ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aHET4S5L004-1.fastq.gz", "fastq", 698753836.0, 9249820.0, "E MTAB 11505:sv2aHET4S5L004", "0:75.54 1:0", "A:164809473;C:171057633;G:170349692;T:192441413;N:95625", 75, 0, null, null, 164809473, 171057633, 170349692, 192441413, 95625, "ERX8620328", "ERS10857216", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.95613, null, 0.09885, null, 0.65798, null, 0.47227, null, 76, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10443, "ERR9077166", "ERX8620327", "ERS10857215", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aHET3S4", "SAMEA13254172", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254172|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET3S4|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET3S4|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aHET3S4 s", "Sv2aHET3S4 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a+/ ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aHET3S4L001-1.fastq.gz", "fastq", 691298605.0, 9151512.0, "E MTAB 11505:sv2aHET3S4L001", "0:75.54 1:0", "A:164743919;C:170752099;G:164763621;T:190912044;N:126922", 75, 0, null, null, 164743919, 170752099, 164763621, 190912044, 126922, "ERX8620327", "ERS10857215", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.95684, null, 0.09604, null, 0.6603, null, 0.47285, null, 76, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10444, "ERR9077167", "ERX8620327", "ERS10857215", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aHET3S4", "SAMEA13254172", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254172|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET3S4|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET3S4|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aHET3S4 s", "Sv2aHET3S4 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a+/ ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aHET3S4L002-1.fastq.gz", "fastq", 694080975.0, 9188259.0, "E MTAB 11505:sv2aHET3S4L002", "0:75.54 1:0", "A:165336810;C:171397273;G:165632321;T:191591872;N:122699", 75, 0, null, null, 165336810, 171397273, 165632321, 191591872, 122699, "ERX8620327", "ERS10857215", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.95612, null, 0.09694, null, 0.65993, null, 0.46918, null, 76, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10445, "ERR9077168", "ERX8620327", "ERS10857215", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aHET3S4", "SAMEA13254172", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254172|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET3S4|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET3S4|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aHET3S4 s", "Sv2aHET3S4 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  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Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aHET3S4", "SAMEA13254172", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254172|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET3S4|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET3S4|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aHET3S4 s", "Sv2aHET3S4 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  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Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aHET2S3", "SAMEA13254171", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254171|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET2S3|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET2S3|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aHET2S3 s", "Sv2aHET2S3 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  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Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aHET2S3", "SAMEA13254171", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254171|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET2S3|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET2S3|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aHET2S3 s", "Sv2aHET2S3 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  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Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aHET2S3", "SAMEA13254171", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254171|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET2S3|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET2S3|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aHET2S3 s", "Sv2aHET2S3 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  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Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aHET2S3", "SAMEA13254171", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254171|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET2S3|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET2S3|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aHET2S3 s", "Sv2aHET2S3 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a+/ ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aHET2S3L004-1.fastq.gz", "fastq", 747723481.0, 9898304.0, "E MTAB 11505:sv2aHET2S3L004", "0:75.54 1:0", "A:176935451;C:184684782;G:179046040;T:206946124;N:111084", 75, 0, null, null, 176935451, 184684782, 179046040, 206946124, 111084, "ERX8620326", "ERS10857214", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.95934, null, 0.08676, null, 0.65533, null, 0.47523, null, 75, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [11025, "ERR9787516", "ERX9334792", "ERS12088364", "ERP137937", "PRJEB53153", "RNA seq of depdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae", "E-MTAB-11776", "Transcriptome Analysis", "Transcriptome analysis of the heads of 5 dpf epdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12", null, "Protocols: 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.", "Sample 9", "SAMEA14460887", "Laboratory for Molecular Biodiscovery, KU Leuven", "ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12|External Id:SAMEA14460887|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven|INSDC first public:2022 09 12T12:19:24Z|INSDC last update:2022 09 12T12:19:24Z|INSDC status:public|Submitter Id:E MTAB 11776:Sample 9|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:tsc2 / |individual:pool of 10 larvae|isolate:not applicable|organism part:head|sample name:E MTAB 11776:Sample 9|scientific name:Danio rerio|sex:non available|strain:AB", null, null, null, null, null, null, null, null, "Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae", "E MTAB 11776:Sample 9 p", "Sample 9 p", "RNA seq of depdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae", "5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.", "Experimental Factor: genotype:tsc2 / ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP137937", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae", "ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12", "TSCHOM1_1.fastq.gz TSCHOM1_2.fastq.gz", "fastq fastq", 6360351174.0, 31486887.0, "E MTAB 11776:TSCHOM1 ", "0:101 1:101", "A:1750731340;C:1410426576;G:1460021447;T:1739131898;N:39913", 101, 101, null, null, 1750731340, 1410426576, 1460021447, 1739131898, 39913, "ERX9334792", "ERS12088364", "ERA14756702", "Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive", 2, 0.93517, 0.92417, 0.15551, 0.15199, 0.67736, 0.6786, 0.48763, 0.48988, 101, 101, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-09-12", "Larval", "Larval", "Head", "Nervous System"], [11026, "ERR9787515", "ERX9334791", "ERS12088363", "ERP137937", "PRJEB53153", "RNA seq of depdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae", "E-MTAB-11776", "Transcriptome Analysis", "Transcriptome analysis of the heads of 5 dpf epdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12", null, "Protocols: 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.", "Sample 8", "SAMEA14460886", "Laboratory for Molecular Biodiscovery, KU Leuven", "ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12|External Id:SAMEA14460886|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven|INSDC first public:2022 09 12T12:19:24Z|INSDC last update:2022 09 12T12:19:24Z|INSDC status:public|Submitter Id:E MTAB 11776:Sample 8|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:depdc5 / |individual:pool of 10 larvae|isolate:not applicable|organism part:head|sample name:E MTAB 11776:Sample 8|scientific name:Danio rerio|sex:non available|strain:AB", null, null, null, null, null, null, null, null, "Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae", "E MTAB 11776:Sample 8 p", "Sample 8 p", "RNA seq of depdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae", "5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.", "Experimental Factor: genotype:depdc5 / ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP137937", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae", "ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12", "DEPHOM4_1.fastq.gz DEPHOM4_2.fastq.gz", "fastq fastq", 6195443828.0, 30670514.0, "E MTAB 11776:DEPHOM4 ", "0:101 1:101", "A:1524344281;C:1573560980;G:1585093004;T:1512406843;N:38720", 101, 101, null, null, 1524344281, 1573560980, 1585093004, 1512406843, 38720, "ERX9334791", "ERS12088363", "ERA14756702", "Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive", 2, 0.95088, 0.9542, 0.17631, 0.17203, 0.71121, 0.70981, 0.56015, 0.57999, 101, 101, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-09-12", "Larval", "Larval", "Head", "Nervous System"], [11027, "ERR9787514", "ERX9334790", "ERS12088362", "ERP137937", "PRJEB53153", "RNA seq of depdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae", "E-MTAB-11776", "Transcriptome Analysis", "Transcriptome analysis of the heads of 5 dpf epdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12", null, "Protocols: 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.", "Sample 7", "SAMEA14460885", "Laboratory for Molecular Biodiscovery, KU Leuven", "ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12|External Id:SAMEA14460885|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven|INSDC first public:2022 09 12T12:19:23Z|INSDC last update:2022 09 12T12:19:23Z|INSDC status:public|Submitter Id:E MTAB 11776:Sample 7|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:depdc5 / |individual:pool of 10 larvae|isolate:not applicable|organism part:head|sample name:E MTAB 11776:Sample 7|scientific name:Danio rerio|sex:non available|strain:AB", null, null, null, null, null, null, null, null, "Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae", "E MTAB 11776:Sample 7 p", "Sample 7 p", "RNA seq of depdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae", "5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.", "Experimental Factor: genotype:depdc5 / ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP137937", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae", "ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12", "DEPHOM7_1.fastq.gz DEPHOM7_2.fastq.gz", "fastq fastq", 6201660378.0, 30701289.0, "E MTAB 11776:DEPHOM7 ", "0:101 1:101", "A:1699906420;C:1382805098;G:1427335171;T:1691574092;N:39597", 101, 101, null, null, 1699906420, 1382805098, 1427335171, 1691574092, 39597, "ERX9334790", "ERS12088362", "ERA14756702", "Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive", 2, 0.92781, 0.92603, 0.15556, 0.15416, 0.67965, 0.67963, 0.48209, 0.48229, 101, 101, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-09-12", "Larval", "Larval", "Head", "Nervous System"], [11028, "ERR9787513", "ERX9334789", "ERS12088361", "ERP137937", "PRJEB53153", "RNA seq of depdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae", "E-MTAB-11776", "Transcriptome Analysis", "Transcriptome analysis of the heads of 5 dpf epdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12", null, "Protocols: 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.", "Sample 6", "SAMEA14460884", "Laboratory for Molecular Biodiscovery, KU Leuven", "ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12|External Id:SAMEA14460884|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven|INSDC first public:2022 09 12T12:19:23Z|INSDC last update:2022 09 12T12:19:23Z|INSDC status:public|Submitter Id:E MTAB 11776:Sample 6|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:depdc5 / |individual:pool of 10 larvae|isolate:not applicable|organism part:head|sample name:E MTAB 11776:Sample 6|scientific name:Danio rerio|sex:non available|strain:AB", null, null, null, null, null, null, null, null, "Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae", "E MTAB 11776:Sample 6 p", "Sample 6 p", "RNA seq of depdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae", "5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.", "Experimental Factor: genotype:depdc5 / ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP137937", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae", "ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12", "DEPHOM6_1.fastq.gz DEPHOM6_2.fastq.gz", "fastq fastq", 6255857382.0, 30969591.0, "E MTAB 11776:DEPHOM6 ", "0:101 1:101", "A:1722554517;C:1398134491;G:1424183345;T:1710945949;N:39080", 101, 101, null, null, 1722554517, 1398134491, 1424183345, 1710945949, 39080, "ERX9334789", "ERS12088361", "ERA14756702", "Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive", 2, 0.93476, 0.93206, 0.16121, 0.1592, 0.67882, 0.67898, 0.48043, 0.47851, 101, 101, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-09-12", "Larval", "Larval", "Head", "Nervous System"], [11029, "ERR9787512", "ERX9334788", "ERS12088360", "ERP137937", "PRJEB53153", "RNA seq of depdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae", "E-MTAB-11776", "Transcriptome Analysis", "Transcriptome analysis of the heads of 5 dpf epdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12", null, "Protocols: 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.", "Sample 5", "SAMEA14460883", "Laboratory for Molecular Biodiscovery, KU Leuven", "ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12|External Id:SAMEA14460883|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven|INSDC first public:2022 09 12T12:19:23Z|INSDC last update:2022 09 12T12:19:23Z|INSDC status:public|Submitter Id:E MTAB 11776:Sample 5|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:depdc5 / |individual:pool of 10 larvae|isolate:not applicable|organism part:head|sample name:E MTAB 11776:Sample 5|scientific name:Danio rerio|sex:non available|strain:AB", null, null, null, null, null, null, null, null, "Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae", "E MTAB 11776:Sample 5 p", "Sample 5 p", "RNA seq of depdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae", "5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.", "Experimental Factor: genotype:depdc5 / ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP137937", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae", "ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12", "DEPHOM1_1.fastq.gz DEPHOM1_2.fastq.gz", "fastq fastq", 6340417006.0, 31388203.0, "E MTAB 11776:DEPHOM1 ", "0:101 1:101", "A:1738622221;C:1420060351;G:1455477148;T:1726216833;N:40453", 101, 101, null, null, 1738622221, 1420060351, 1455477148, 1726216833, 40453, "ERX9334788", "ERS12088360", "ERA14756702", "Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive", 2, 0.93634, 0.93167, 0.15036, 0.14813, 0.67809, 0.67813, 0.47978, 0.47851, 101, 101, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-09-12", "Larval", "Larval", "Head", "Nervous System"], [11030, "ERR9787511", "ERX9334787", "ERS12088359", "ERP137937", "PRJEB53153", "RNA seq of depdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae", "E-MTAB-11776", "Transcriptome Analysis", "Transcriptome analysis of the heads of 5 dpf epdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12", null, "Protocols: 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.", "Sample 4", "SAMEA14460882", "Laboratory for Molecular Biodiscovery, KU Leuven", "ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12|External Id:SAMEA14460882|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven|INSDC first public:2022 09 12T12:19:23Z|INSDC last update:2022 09 12T12:19:23Z|INSDC status:public|Submitter Id:E MTAB 11776:Sample 4|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:depdc5 /  x tsc2 / |individual:pool of 10 larvae|isolate:not applicable|organism part:head|sample name:E MTAB 11776:Sample 4|scientific name:Danio rerio|sex:non available|strain:AB", null, null, null, null, null, null, null, null, "Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae", "E MTAB 11776:Sample 4 p", "Sample 4 p", "RNA seq of depdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae", "5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.", "Experimental Factor: genotype:depdc5 /  x tsc2 / ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP137937", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae", "ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12", "2XHOM7_1.fastq.gz 2XHOM7_2.fastq.gz", "fastq fastq", 6291880446.0, 31147923.0, "E MTAB 11776:2XHOM7 ", "0:101 1:101", "A:1712784902;C:1424143534;G:1452943449;T:1701969086;N:39475", 101, 101, null, null, 1712784902, 1424143534, 1452943449, 1701969086, 39475, "ERX9334787", "ERS12088359", "ERA14756702", "Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive", 2, 0.93869, 0.93511, 0.15405, 0.15042, 0.67377, 0.67351, 0.50599, 0.50683, 101, 101, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-09-12", "Larval", "Larval", "Head", "Nervous System"], [11031, "ERR9787510", "ERX9334786", "ERS12088358", "ERP137937", "PRJEB53153", "RNA seq of depdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae", "E-MTAB-11776", "Transcriptome Analysis", "Transcriptome analysis of the heads of 5 dpf epdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12", null, "Protocols: 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.", "Sample 3", "SAMEA14460881", "Laboratory for Molecular Biodiscovery, KU Leuven", "ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12|External Id:SAMEA14460881|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven|INSDC first public:2022 09 12T12:19:23Z|INSDC last update:2022 09 12T12:19:23Z|INSDC status:public|Submitter Id:E MTAB 11776:Sample 3|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:depdc5 /  x tsc2 / |individual:pool of 10 larvae|isolate:not applicable|organism part:head|sample name:E MTAB 11776:Sample 3|scientific name:Danio rerio|sex:non available|strain:AB", null, null, null, null, null, null, null, null, "Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae", "E MTAB 11776:Sample 3 p", "Sample 3 p", "RNA seq of depdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae", "5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.", "Experimental Factor: genotype:depdc5 /  x tsc2 / ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP137937", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae", "ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12", "2XHOM6_1.fastq.gz 2XHOM6_2.fastq.gz", "fastq fastq", 6214327596.0, 30763998.0, "E MTAB 11776:2XHOM6 ", "0:101 1:101", "A:1705019759;C:1384064144;G:1432455427;T:1692749964;N:38302", 101, 101, null, null, 1705019759, 1384064144, 1432455427, 1692749964, 38302, "ERX9334786", "ERS12088358", "ERA14756702", "Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive", 2, 0.93591, 0.92636, 0.14492, 0.14145, 0.67489, 0.67491, 0.47753, 0.49286, 101, 101, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-09-12", "Larval", "Larval", "Head", "Nervous System"], [11032, "ERR9787509", "ERX9334785", "ERS12088357", "ERP137937", "PRJEB53153", "RNA seq of depdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae", "E-MTAB-11776", "Transcriptome Analysis", "Transcriptome analysis of the heads of 5 dpf epdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12", null, "Protocols: 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.", "Sample 2", "SAMEA14460880", "Laboratory for Molecular Biodiscovery, KU Leuven", "ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12|External Id:SAMEA14460880|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven|INSDC first public:2022 09 12T12:19:23Z|INSDC last update:2022 09 12T12:19:23Z|INSDC status:public|Submitter Id:E MTAB 11776:Sample 2|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:depdc5 /  x tsc2 / |individual:pool of 10 larvae|isolate:not applicable|organism part:head|sample name:E MTAB 11776:Sample 2|scientific name:Danio rerio|sex:non available|strain:AB", null, null, null, null, null, null, null, null, "Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae", "E MTAB 11776:Sample 2 p", "Sample 2 p", "RNA seq of depdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae", "5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.", "Experimental Factor: genotype:depdc5 /  x tsc2 / ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP137937", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae", "ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12", "2XHOM4_1.fastq.gz 2XHOM4_2.fastq.gz", "fastq fastq", 6301691990.0, 31196495.0, "E MTAB 11776:2XHOM4 ", "0:101 1:101", "A:1698118705;C:1442276237;G:1473988715;T:1687268823;N:39510", 101, 101, null, null, 1698118705, 1442276237, 1473988715, 1687268823, 39510, "ERX9334785", "ERS12088357", "ERA14756702", "Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive", 2, 0.94255, 0.93839, 0.13232, 0.13007, 0.67326, 0.67294, 0.49084, 0.49291, 101, 101, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-09-12", "Larval", "Larval", "Head", "Nervous System"], [11033, "ERR9787508", "ERX9334784", "ERS12088356", "ERP137937", "PRJEB53153", "RNA seq of depdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae", "E-MTAB-11776", "Transcriptome Analysis", "Transcriptome analysis of the heads of 5 dpf epdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12", null, "Protocols: 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.", "Sample 16", "SAMEA14460879", "Laboratory for Molecular Biodiscovery, KU Leuven", "ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12|External Id:SAMEA14460879|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven|INSDC first public:2022 09 12T12:19:23Z|INSDC last update:2022 09 12T12:19:23Z|INSDC status:public|Submitter Id:E MTAB 11776:Sample 16|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:wild type genotype|individual:pool of 10 larvae|isolate:not applicable|organism part:head|sample name:E MTAB 11776:Sample 16|scientific name:Danio rerio|sex:non available|strain:AB", null, null, null, null, null, null, null, null, "Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae", "E MTAB 11776:Sample 16 p", "Sample 16 p", "RNA seq of depdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae", "5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.", "Experimental Factor: genotype:wild type genotype", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP137937", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae", "ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12", "WT7_1.fastq.gz WT7_2.fastq.gz", "fastq fastq", 6206666746.0, 30726073.0, "E MTAB 11776:WT7 ", "0:101 1:101", "A:1705689174;C:1387290312;G:1420249657;T:1693397929;N:39674", 101, 101, null, null, 1705689174, 1387290312, 1420249657, 1693397929, 39674, "ERX9334784", "ERS12088356", "ERA14756702", "Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive", 2, 0.93493, 0.92964, 0.15803, 0.1554, 0.67405, 0.67501, 0.47988, 0.46768, 101, 101, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-09-12", "Larval", "Larval", "Head", "Nervous System"], [11034, "ERR9787507", "ERX9334783", "ERS12088355", "ERP137937", "PRJEB53153", "RNA seq of depdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae", "E-MTAB-11776", "Transcriptome Analysis", "Transcriptome analysis of the heads of 5 dpf epdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12", null, "Protocols: 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.", "Sample 15", "SAMEA14460878", "Laboratory for Molecular Biodiscovery, KU Leuven", "ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12|External Id:SAMEA14460878|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven|INSDC first public:2022 09 12T12:19:23Z|INSDC last update:2022 09 12T12:19:23Z|INSDC status:public|Submitter Id:E MTAB 11776:Sample 15|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:wild type genotype|individual:pool of 10 larvae|isolate:not applicable|organism part:head|sample name:E MTAB 11776:Sample 15|scientific name:Danio rerio|sex:non available|strain:AB", null, null, null, null, null, null, null, null, "Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae", "E MTAB 11776:Sample 15 p", "Sample 15 p", "RNA seq of depdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae", "5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.", "Experimental Factor: genotype:wild type genotype", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP137937", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae", "ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12", "WT6_1.fastq.gz WT6_2.fastq.gz", "fastq fastq", 6360740630.0, 31488815.0, "E MTAB 11776:WT6 ", "0:101 1:101", "A:1735195643;C:1436805269;G:1469558083;T:1719141699;N:39936", 101, 101, null, null, 1735195643, 1436805269, 1469558083, 1719141699, 39936, "ERX9334783", "ERS12088355", "ERA14756702", "Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive", 2, 0.93629, 0.93343, 0.15169, 0.1501, 0.67572, 0.67517, 0.47499, 0.47879, 101, 101, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-09-12", "Larval", "Larval", "Head", "Nervous System"], [11035, "ERR9787506", "ERX9334782", "ERS12088354", "ERP137937", "PRJEB53153", "RNA seq of depdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae", "E-MTAB-11776", "Transcriptome Analysis", "Transcriptome analysis of the heads of 5 dpf epdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12", null, "Protocols: 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.", "Sample 14", "SAMEA14460877", "Laboratory for Molecular Biodiscovery, KU Leuven", "ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12|External Id:SAMEA14460877|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven|INSDC first public:2022 09 12T12:19:23Z|INSDC last update:2022 09 12T12:19:23Z|INSDC status:public|Submitter Id:E MTAB 11776:Sample 14|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:wild type genotype|individual:pool of 10 larvae|isolate:not applicable|organism part:head|sample name:E MTAB 11776:Sample 14|scientific name:Danio rerio|sex:non available|strain:AB", null, null, null, null, null, null, null, null, "Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae", "E MTAB 11776:Sample 14 p", "Sample 14 p", "RNA seq of depdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae", "5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.", "Experimental Factor: genotype:wild type genotype", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP137937", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae", "ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12", "WT4_1.fastq.gz WT4_2.fastq.gz", "fastq fastq", 6227472342.0, 30829071.0, "E MTAB 11776:WT4 ", "0:101 1:101", "A:1668352581;C:1434152947;G:1468645621;T:1656281986;N:39207", 101, 101, null, null, 1668352581, 1434152947, 1468645621, 1656281986, 39207, "ERX9334782", "ERS12088354", "ERA14756702", "Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive", 2, 0.94261, 0.93675, 0.15334, 0.15049, 0.67927, 0.67872, 0.48762, 0.49138, 101, 101, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-09-12", "Larval", "Larval", "Head", "Nervous System"], [11036, "ERR9787505", "ERX9334781", "ERS12088353", "ERP137937", "PRJEB53153", "RNA seq of depdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae", "E-MTAB-11776", "Transcriptome Analysis", "Transcriptome analysis of the heads of 5 dpf epdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12", null, "Protocols: 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.", "Sample 13", "SAMEA14460876", "Laboratory for Molecular Biodiscovery, KU Leuven", "ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12|External Id:SAMEA14460876|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven|INSDC first public:2022 09 12T12:19:23Z|INSDC last update:2022 09 12T12:19:23Z|INSDC status:public|Submitter Id:E MTAB 11776:Sample 13|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:wild type genotype|individual:pool of 10 larvae|isolate:not applicable|organism part:head|sample name:E MTAB 11776:Sample 13|scientific name:Danio rerio|sex:non available|strain:AB", null, null, null, null, null, null, null, null, "Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae", "E MTAB 11776:Sample 13 p", "Sample 13 p", "RNA seq of depdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae", "5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.", "Experimental Factor: genotype:wild type genotype", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP137937", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae", "ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12", "WT1_1.fastq.gz WT1_2.fastq.gz", "fastq fastq", 6332757570.0, 31350285.0, "E MTAB 11776:WT1 ", "0:101 1:101", "A:1726838066;C:1431452165;G:1460746594;T:1713681043;N:39702", 101, 101, null, null, 1726838066, 1431452165, 1460746594, 1713681043, 39702, "ERX9334781", "ERS12088353", "ERA14756702", "Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive", 2, 0.92422, 0.93556, 0.14884, 0.14926, 0.67694, 0.67564, 0.48223, 0.48884, 101, 101, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-09-12", "Larval", "Larval", "Head", "Nervous System"], [11037, "ERR9787504", "ERX9334780", "ERS12088352", "ERP137937", "PRJEB53153", "RNA seq of depdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae", "E-MTAB-11776", "Transcriptome Analysis", "Transcriptome analysis of the heads of 5 dpf epdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12", null, "Protocols: 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.", "Sample 12", "SAMEA14460875", "Laboratory for Molecular Biodiscovery, KU Leuven", "ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12|External Id:SAMEA14460875|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven|INSDC first public:2022 09 12T12:19:23Z|INSDC last update:2022 09 12T12:19:23Z|INSDC status:public|Submitter Id:E MTAB 11776:Sample 12|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:tsc2 / |individual:pool of 10 larvae|isolate:not applicable|organism part:head|sample name:E MTAB 11776:Sample 12|scientific name:Danio rerio|sex:non available|strain:AB", null, null, null, null, null, null, null, null, "Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae", "E MTAB 11776:Sample 12 p", "Sample 12 p", "RNA seq of depdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae", "5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.", "Experimental Factor: genotype:tsc2 / ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP137937", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae", "ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12", "TSCHOM7_1.fastq.gz TSCHOM7_2.fastq.gz", "fastq fastq", 6216990360.0, 30777180.0, "E MTAB 11776:TSCHOM7 ", "0:101 1:101", "A:1710256020;C:1390027205;G:1419250792;T:1697417099;N:39244", 101, 101, null, null, 1710256020, 1390027205, 1419250792, 1697417099, 39244, "ERX9334780", "ERS12088352", "ERA14756702", "Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive", 2, 0.93162, 0.92801, 0.15191, 0.14943, 0.67789, 0.67951, 0.47915, 0.47762, 101, 101, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-09-12", "Larval", "Larval", "Head", "Nervous System"], [11038, "ERR9787503", "ERX9334779", "ERS12088351", "ERP137937", "PRJEB53153", "RNA seq of depdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae", "E-MTAB-11776", "Transcriptome Analysis", "Transcriptome analysis of the heads of 5 dpf epdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12", null, "Protocols: 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.", "Sample 11", "SAMEA14460874", "Laboratory for Molecular Biodiscovery, KU Leuven", "ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12|External Id:SAMEA14460874|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven|INSDC first public:2022 09 12T12:19:23Z|INSDC last update:2022 09 12T12:19:23Z|INSDC status:public|Submitter Id:E MTAB 11776:Sample 11|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:tsc2 / |individual:pool of 10 larvae|isolate:not applicable|organism part:head|sample name:E MTAB 11776:Sample 11|scientific name:Danio rerio|sex:non available|strain:AB", null, null, null, null, null, null, null, null, "Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae", "E MTAB 11776:Sample 11 p", "Sample 11 p", "RNA seq of depdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae", "5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.", "Experimental Factor: genotype:tsc2 / ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP137937", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae", "ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12", "TSCHOM6_1.fastq.gz TSCHOM6_2.fastq.gz", "fastq fastq", 6199081848.0, 30688524.0, "E MTAB 11776:TSCHOM6 ", "0:101 1:101", "A:1705859045;C:1387530558;G:1415224718;T:1690428701;N:38826", 101, 101, null, null, 1705859045, 1387530558, 1415224718, 1690428701, 38826, "ERX9334779", "ERS12088351", "ERA14756702", "Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive", 2, 0.93583, 0.93349, 0.15923, 0.15664, 0.67976, 0.67986, 0.48632, 0.48376, 101, 101, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-09-12", "Larval", "Larval", "Head", "Nervous System"], [11039, "ERR9787502", "ERX9334778", "ERS12088350", "ERP137937", "PRJEB53153", "RNA seq of depdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae", "E-MTAB-11776", "Transcriptome Analysis", "Transcriptome analysis of the heads of 5 dpf epdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12", null, "Protocols: 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.", "Sample 10", "SAMEA14460873", "Laboratory for Molecular Biodiscovery, KU Leuven", "ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12|External Id:SAMEA14460873|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven|INSDC first public:2022 09 12T12:19:23Z|INSDC last update:2022 09 12T12:19:23Z|INSDC status:public|Submitter Id:E MTAB 11776:Sample 10|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:tsc2 / |individual:pool of 10 larvae|isolate:not applicable|organism part:head|sample name:E MTAB 11776:Sample 10|scientific name:Danio rerio|sex:non available|strain:AB", null, null, null, null, null, null, null, null, "Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae", "E MTAB 11776:Sample 10 p", "Sample 10 p", "RNA seq of depdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae", "5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.", "Experimental Factor: genotype:tsc2 / ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP137937", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae", "ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12", "TSCHOM4_1.fastq.gz TSCHOM4_2.fastq.gz", "fastq fastq", 6287836204.0, 31127902.0, "E MTAB 11776:TSCHOM4 ", "0:101 1:101", "A:1673762523;C:1453880149;G:1493343671;T:1666809849;N:40012", 101, 101, null, null, 1673762523, 1453880149, 1493343671, 1666809849, 40012, "ERX9334778", "ERS12088350", "ERA14756702", "Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive", 2, 0.94269, 0.9375, 0.14166, 0.13979, 0.67476, 0.67722, 0.4927, 0.49365, 101, 101, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-09-12", "Larval", "Larval", "Head", "Nervous System"], [11040, "ERR9787501", "ERX9334777", "ERS12088349", "ERP137937", "PRJEB53153", "RNA seq of depdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae", "E-MTAB-11776", "Transcriptome Analysis", "Transcriptome analysis of the heads of 5 dpf epdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12", null, "Protocols: 5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.", "Sample 1", "SAMEA14460872", "Laboratory for Molecular Biodiscovery, KU Leuven", "ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12|External Id:SAMEA14460872|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven|INSDC first public:2022 09 12T12:19:23Z|INSDC last update:2022 09 12T12:19:23Z|INSDC status:public|Submitter Id:E MTAB 11776:Sample 1|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:depdc5 /  x tsc2 / |individual:pool of 10 larvae|isolate:not applicable|organism part:head|sample name:E MTAB 11776:Sample 1|scientific name:Danio rerio|sex:non available|strain:AB", null, null, null, null, null, null, null, null, "Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae", "E MTAB 11776:Sample 1 p", "Sample 1 p", "RNA seq of depdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae", "5 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled in quadruplicate per genotype. otal RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. Illumina TruSeq Stranded mRNA Seq sample preparation kit was used to prepare the mRNA sequencing library according to the manufacturer guidelines.", "Experimental Factor: genotype:depdc5 /  x tsc2 / ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP137937", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of depdc5 /   tsc2 /   depdc5 /  x tsc2 /  and wildtype zebrafish larvae", "ENA FIRST PUBLIC:2022 09 12|ENA LAST UPDATE:2022 09 12", "2XHOM1_1.fastq.gz 2XHOM1_2.fastq.gz", "fastq fastq", 6232605768.0, 30854484.0, "E MTAB 11776:2XHOM1 ", "0:101 1:101", "A:1725401812;C:1384507160;G:1410306583;T:1712351429;N:38784", 101, 101, null, null, 1725401812, 1384507160, 1410306583, 1712351429, 38784, "ERX9334777", "ERS12088349", "ERA14756702", "Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven|European Nucleotide Archive", 2, 0.93594, 0.93262, 0.15282, 0.14977, 0.67503, 0.67373, 0.49522, 0.49759, 101, 101, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-09-12", "Larval", "Larval", "Head", "Nervous System"], [32037, "SRR28992909", "SRX24520261", "SRS21267950", "SRP506997", "PRJNA1110229", "Zebrafish models of candidate human epilepsy associated genes provide evidence of hyperexcitability", "PRJNA1110229", "Other", "Hundreds of novel candidate human epilepsy associated genes have been identified thanks to advancements in next generation sequencing and large genome wide association studies  but establishing genetic etiology requires functional validation. We generated a list of >2200 candidate epilepsy associated genes  of which 81 were determined suitable for the generation of loss of function zebrafish models via CRISPR/Cas9 gene editing. Of those 81 crispants  48 were successfully established as stable mutant lines and assessed for seizure like swim patterns in a primary F2 screen. Evidence of seizure like behavior was present in 5 arfgef1  kcnd2  kcnv1  ubr5  wnt8b of the 48 mutant lines assessed. Further characterization of those 5 lines provided evidence for epileptiform activity via electrophysiology in kcnd2 and wnt8b mutants. Additionally  arfgef1 and wnt8b mutants showed a decrease in the number of inhibitory interneurons in the optic tectum of larval animals. Furthermore  RNAseq revealed convergent transcriptional abnormalities between mutant lines  consistent with their developmental defects and hyperexcitable phenotypes. These zebrafish models provide strongest experimental evidence supporting the role of ARFGEF1  KCND2  and WNT8B in human epilepsy and further demonstrate the utility of this model system for evaluating candidate human epilepsy genes.", null, null, null, null, "Kcnd2 Wt1", null, "strain:Casper|age:5dpf|collection date:2022 12 09|geo loc name:USA: Boston  MA|sex:not applicable|tissue:Head|genotype:Kcnd2 Wt1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio Rerio: pools of isolated larval head", "Kcnd2 Wt1", "Kcnd2 Wt1", "Three replicate larvae from Het in crosses  aged 5 dpf  were sacrificed on ice and stored in RNAlater Stabilization Solution according to product specifications. Larval heads were isolated from bodies  which were used for Sanger sequencing for genotyping. Heads were pooled in groups of 20 25 according to genotype and RNA was isolated using a Qiagen RNeasy plus kit according to specifications. QC was performed via Agilent 2100 bioanalyzer  and only samples with an RNA integrity number value of >5.8 were used for library preparation.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "SINGLE", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP506997", null, null, "Kcnd2_Wt1.fq.gz", "fastq", 3386455050.0, 22576367.0, "Kcnd2 Wt1.fq.gz", "0:150", "A:967137430;C:730108913;G:738646978;T:950549427;N:12302", 150, null, null, null, 967137430, 730108913, 738646978, 950549427, 12302, "SRX24520261", "SRS21267950", "SRA1863814", "Boston Childrens Hospital|Neurobiology", "Boston Childrens Hospital", null, null, null, null, null, null, null, null, null, null, null, "B", null, "usable mapping rate", "illumina", "novaseq_era", "unknown", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-05-10", "Larval", "Larval", "Head", "Nervous System"], [32038, "SRR28992910", "SRX24520260", "SRS21267949", "SRP506997", "PRJNA1110229", "Zebrafish models of candidate human epilepsy associated genes provide evidence of hyperexcitability", "PRJNA1110229", "Other", "Hundreds of novel candidate human epilepsy associated genes have been identified thanks to advancements in next generation sequencing and large genome wide association studies  but establishing genetic etiology requires functional validation. We generated a list of >2200 candidate epilepsy associated genes  of which 81 were determined suitable for the generation of loss of function zebrafish models via CRISPR/Cas9 gene editing. Of those 81 crispants  48 were successfully established as stable mutant lines and assessed for seizure like swim patterns in a primary F2 screen. Evidence of seizure like behavior was present in 5 arfgef1  kcnd2  kcnv1  ubr5  wnt8b of the 48 mutant lines assessed. Further characterization of those 5 lines provided evidence for epileptiform activity via electrophysiology in kcnd2 and wnt8b mutants. Additionally  arfgef1 and wnt8b mutants showed a decrease in the number of inhibitory interneurons in the optic tectum of larval animals. Furthermore  RNAseq revealed convergent transcriptional abnormalities between mutant lines  consistent with their developmental defects and hyperexcitable phenotypes. These zebrafish models provide strongest experimental evidence supporting the role of ARFGEF1  KCND2  and WNT8B in human epilepsy and further demonstrate the utility of this model system for evaluating candidate human epilepsy genes.", null, null, null, null, "Arfgef1 Hom3", null, "strain:Casper|age:5dpf|collection date:2022 12 09|geo loc name:USA: Boston  MA|sex:not applicable|tissue:Head|genotype:Arfgef1 Hom3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio Rerio: pools of isolated larval head", "Arfgef1 Hom3", "Arfgef1 Hom3", "Three replicate larvae from Het in crosses  aged 5 dpf  were sacrificed on ice and stored in RNAlater Stabilization Solution according to product specifications. Larval heads were isolated from bodies  which were used for Sanger sequencing for genotyping. Heads were pooled in groups of 20 25 according to genotype and RNA was isolated using a Qiagen RNeasy plus kit according to specifications. QC was performed via Agilent 2100 bioanalyzer  and only samples with an RNA integrity number value of >5.8 were used for library preparation.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "SINGLE", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP506997", null, null, "Arfgef1_Hom3.fq.gz", "fastq", 3647365500.0, 24315770.0, "Arfgef1 Hom3.fq.gz", "0:150", "A:923919033;C:903440333;G:911845870;T:908147370;N:12894", 150, null, null, null, 923919033, 903440333, 911845870, 908147370, 12894, "SRX24520260", "SRS21267949", "SRA1863814", "Boston Childrens Hospital|Neurobiology", "Boston Childrens Hospital", null, null, null, null, null, null, null, null, null, null, null, "B", null, "usable mapping rate", "illumina", "novaseq_era", "unknown", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-05-10", "Larval", "Larval", "Head", "Nervous System"], [32039, "SRR28992915", "SRX24520258", "SRS21267947", "SRP506997", "PRJNA1110229", "Zebrafish models of candidate human epilepsy associated genes provide evidence of hyperexcitability", "PRJNA1110229", "Other", "Hundreds of novel candidate human epilepsy associated genes have been identified thanks to advancements in next generation sequencing and large genome wide association studies  but establishing genetic etiology requires functional validation. We generated a list of >2200 candidate epilepsy associated genes  of which 81 were determined suitable for the generation of loss of function zebrafish models via CRISPR/Cas9 gene editing. Of those 81 crispants  48 were successfully established as stable mutant lines and assessed for seizure like swim patterns in a primary F2 screen. Evidence of seizure like behavior was present in 5 arfgef1  kcnd2  kcnv1  ubr5  wnt8b of the 48 mutant lines assessed. Further characterization of those 5 lines provided evidence for epileptiform activity via electrophysiology in kcnd2 and wnt8b mutants. Additionally  arfgef1 and wnt8b mutants showed a decrease in the number of inhibitory interneurons in the optic tectum of larval animals. Furthermore  RNAseq revealed convergent transcriptional abnormalities between mutant lines  consistent with their developmental defects and hyperexcitable phenotypes. These zebrafish models provide strongest experimental evidence supporting the role of ARFGEF1  KCND2  and WNT8B in human epilepsy and further demonstrate the utility of this model system for evaluating candidate human epilepsy genes.", null, null, null, null, "Arfgef1 Hom1", null, "strain:Casper|age:5dpf|collection date:2022 12 09|geo loc name:USA: Boston  MA|sex:not applicable|tissue:Head|genotype:Arfgef1 Hom1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio Rerio: pools of isolated larval head", "Arfgef1 Hom1", "Arfgef1 Hom1", "Three replicate larvae from Het in crosses  aged 5 dpf  were sacrificed on ice and stored in RNAlater Stabilization Solution according to product specifications. Larval heads were isolated from bodies  which were used for Sanger sequencing for genotyping. Heads were pooled in groups of 20 25 according to genotype and RNA was isolated using a Qiagen RNeasy plus kit according to specifications. QC was performed via Agilent 2100 bioanalyzer  and only samples with an RNA integrity number value of >5.8 were used for library preparation.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "SINGLE", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP506997", null, null, "Arfgef1_Hom1.fq.gz", "fastq", 3388869300.0, 22592462.0, "Arfgef1 Hom1.fq.gz", "0:150", "A:869162615;C:827706442;G:835453288;T:856534517;N:12438", 150, null, null, null, 869162615, 827706442, 835453288, 856534517, 12438, "SRX24520258", "SRS21267947", "SRA1863814", "Boston Childrens Hospital|Neurobiology", "Boston Childrens Hospital", null, null, null, null, null, null, null, null, null, null, null, "B", null, "usable mapping rate", "illumina", "novaseq_era", "unknown", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-05-10", "Larval", "Larval", "Head", "Nervous System"], [32040, "SRR28992912", "SRX24520257", "SRS21267946", "SRP506997", "PRJNA1110229", "Zebrafish models of candidate human epilepsy associated genes provide evidence of hyperexcitability", "PRJNA1110229", "Other", "Hundreds of novel candidate human epilepsy associated genes have been identified thanks to advancements in next generation sequencing and large genome wide association studies  but establishing genetic etiology requires functional validation. We generated a list of >2200 candidate epilepsy associated genes  of which 81 were determined suitable for the generation of loss of function zebrafish models via CRISPR/Cas9 gene editing. Of those 81 crispants  48 were successfully established as stable mutant lines and assessed for seizure like swim patterns in a primary F2 screen. Evidence of seizure like behavior was present in 5 arfgef1  kcnd2  kcnv1  ubr5  wnt8b of the 48 mutant lines assessed. Further characterization of those 5 lines provided evidence for epileptiform activity via electrophysiology in kcnd2 and wnt8b mutants. Additionally  arfgef1 and wnt8b mutants showed a decrease in the number of inhibitory interneurons in the optic tectum of larval animals. Furthermore  RNAseq revealed convergent transcriptional abnormalities between mutant lines  consistent with their developmental defects and hyperexcitable phenotypes. These zebrafish models provide strongest experimental evidence supporting the role of ARFGEF1  KCND2  and WNT8B in human epilepsy and further demonstrate the utility of this model system for evaluating candidate human epilepsy genes.", null, null, null, null, "Arfgef1 Het3", null, "strain:Casper|age:5dpf|collection date:2022 12 09|geo loc name:USA: Boston  MA|sex:not applicable|tissue:Head|genotype:Arfgef1 Het3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio Rerio: pools of isolated larval head", "Arfgef1 Het3", "Arfgef1 Het3", "Three replicate larvae from Het in crosses  aged 5 dpf  were sacrificed on ice and stored in RNAlater Stabilization Solution according to product specifications. Larval heads were isolated from bodies  which were used for Sanger sequencing for genotyping. Heads were pooled in groups of 20 25 according to genotype and RNA was isolated using a Qiagen RNeasy plus kit according to specifications. QC was performed via Agilent 2100 bioanalyzer  and only samples with an RNA integrity number value of >5.8 were used for library preparation.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "SINGLE", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP506997", null, null, "Arfgef1_Het3.fq.gz", "fastq", 3369996750.0, 22466645.0, "Arfgef1 Het3.fq.gz", "0:150", "A:861941573;C:824864964;G:833793356;T:849388791;N:8066", 150, null, null, null, 861941573, 824864964, 833793356, 849388791, 8066, "SRX24520257", "SRS21267946", "SRA1863814", "Boston Childrens Hospital|Neurobiology", "Boston Childrens Hospital", null, null, null, null, null, null, null, null, null, null, null, "B", null, "usable mapping rate", "illumina", "novaseq_era", "unknown", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-05-10", "Larval", "Larval", "Head", "Nervous System"], [32041, "SRR28992913", "SRX24520256", "SRS21267945", "SRP506997", "PRJNA1110229", "Zebrafish models of candidate human epilepsy associated genes provide evidence of hyperexcitability", "PRJNA1110229", "Other", "Hundreds of novel candidate human epilepsy associated genes have been identified thanks to advancements in next generation sequencing and large genome wide association studies  but establishing genetic etiology requires functional validation. We generated a list of >2200 candidate epilepsy associated genes  of which 81 were determined suitable for the generation of loss of function zebrafish models via CRISPR/Cas9 gene editing. Of those 81 crispants  48 were successfully established as stable mutant lines and assessed for seizure like swim patterns in a primary F2 screen. Evidence of seizure like behavior was present in 5 arfgef1  kcnd2  kcnv1  ubr5  wnt8b of the 48 mutant lines assessed. Further characterization of those 5 lines provided evidence for epileptiform activity via electrophysiology in kcnd2 and wnt8b mutants. Additionally  arfgef1 and wnt8b mutants showed a decrease in the number of inhibitory interneurons in the optic tectum of larval animals. Furthermore  RNAseq revealed convergent transcriptional abnormalities between mutant lines  consistent with their developmental defects and hyperexcitable phenotypes. These zebrafish models provide strongest experimental evidence supporting the role of ARFGEF1  KCND2  and WNT8B in human epilepsy and further demonstrate the utility of this model system for evaluating candidate human epilepsy genes.", null, null, null, null, "Arfgef1 Het2", null, "strain:Casper|age:5dpf|collection date:2022 12 09|geo loc name:USA: Boston  MA|sex:not applicable|tissue:Head|genotype:Arfgef1 Het2|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio Rerio: pools of isolated larval head", "Arfgef1 Het2", "Arfgef1 Het2", "Three replicate larvae from Het in crosses  aged 5 dpf  were sacrificed on ice and stored in RNAlater Stabilization Solution according to product specifications. Larval heads were isolated from bodies  which were used for Sanger sequencing for genotyping. Heads were pooled in groups of 20 25 according to genotype and RNA was isolated using a Qiagen RNeasy plus kit according to specifications. QC was performed via Agilent 2100 bioanalyzer  and only samples with an RNA integrity number value of >5.8 were used for library preparation.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "SINGLE", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP506997", null, null, "Arfgef1_Het2.fq.gz", "fastq", 4755406500.0, 31702710.0, "Arfgef1 Het2.fq.gz", "0:150", "A:1254995507;C:1127923454;G:1138127034;T:1234180808;N:179697", 150, null, null, null, 1254995507, 1127923454, 1138127034, 1234180808, 179697, "SRX24520256", "SRS21267945", "SRA1863814", "Boston Childrens Hospital|Neurobiology", "Boston Childrens Hospital", null, null, null, null, null, null, null, null, null, null, null, "B", null, "usable mapping rate", "illumina", "novaseq_era", "unknown", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-05-10", "Larval", "Larval", "Head", "Nervous System"], [32042, "SRR28992916", "SRX24520254", "SRS21267943", "SRP506997", "PRJNA1110229", "Zebrafish models of candidate human epilepsy associated genes provide evidence of hyperexcitability", "PRJNA1110229", "Other", "Hundreds of novel candidate human epilepsy associated genes have been identified thanks to advancements in next generation sequencing and large genome wide association studies  but establishing genetic etiology requires functional validation. We generated a list of >2200 candidate epilepsy associated genes  of which 81 were determined suitable for the generation of loss of function zebrafish models via CRISPR/Cas9 gene editing. Of those 81 crispants  48 were successfully established as stable mutant lines and assessed for seizure like swim patterns in a primary F2 screen. Evidence of seizure like behavior was present in 5 arfgef1  kcnd2  kcnv1  ubr5  wnt8b of the 48 mutant lines assessed. Further characterization of those 5 lines provided evidence for epileptiform activity via electrophysiology in kcnd2 and wnt8b mutants. Additionally  arfgef1 and wnt8b mutants showed a decrease in the number of inhibitory interneurons in the optic tectum of larval animals. Furthermore  RNAseq revealed convergent transcriptional abnormalities between mutant lines  consistent with their developmental defects and hyperexcitable phenotypes. These zebrafish models provide strongest experimental evidence supporting the role of ARFGEF1  KCND2  and WNT8B in human epilepsy and further demonstrate the utility of this model system for evaluating candidate human epilepsy genes.", null, null, null, null, "Wnt8b Hom3", null, "strain:Casper|age:5dpf|collection date:2022 12 09|geo loc name:USA: Boston  MA|sex:not applicable|tissue:Head|genotype:Wnt8b Hom3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio Rerio: pools of isolated larval head", "Wnt8b Hom3", "Wnt8b Hom3", "Three replicate larvae from Het in crosses  aged 5 dpf  were sacrificed on ice and stored in RNAlater Stabilization Solution according to product specifications. Larval heads were isolated from bodies  which were used for Sanger sequencing for genotyping. Heads were pooled in groups of 20 25 according to genotype and RNA was isolated using a Qiagen RNeasy plus kit according to specifications. QC was performed via Agilent 2100 bioanalyzer  and only samples with an RNA integrity number value of >5.8 were used for library preparation.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "SINGLE", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP506997", null, null, "Wnt8b_Hom3.fq.gz", "fastq", 5283928950.0, 35226193.0, "Wnt8b Hom3.fq.gz", "0:150", "A:1389228037;C:1262004726;G:1265392219;T:1367105548;N:198420", 150, null, null, null, 1389228037, 1262004726, 1265392219, 1367105548, 198420, "SRX24520254", "SRS21267943", "SRA1863814", "Boston Childrens Hospital|Neurobiology", "Boston Childrens Hospital", null, null, null, null, null, null, null, null, null, null, null, "B", null, "usable mapping rate", "illumina", "novaseq_era", "unknown", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-05-10", "Larval", "Larval", "Head", "Nervous System"], [32043, "SRR28992919", "SRX24520251", "SRS21267940", "SRP506997", "PRJNA1110229", "Zebrafish models of candidate human epilepsy associated genes provide evidence of hyperexcitability", "PRJNA1110229", "Other", "Hundreds of novel candidate human epilepsy associated genes have been identified thanks to advancements in next generation sequencing and large genome wide association studies  but establishing genetic etiology requires functional validation. We generated a list of >2200 candidate epilepsy associated genes  of which 81 were determined suitable for the generation of loss of function zebrafish models via CRISPR/Cas9 gene editing. Of those 81 crispants  48 were successfully established as stable mutant lines and assessed for seizure like swim patterns in a primary F2 screen. Evidence of seizure like behavior was present in 5 arfgef1  kcnd2  kcnv1  ubr5  wnt8b of the 48 mutant lines assessed. Further characterization of those 5 lines provided evidence for epileptiform activity via electrophysiology in kcnd2 and wnt8b mutants. Additionally  arfgef1 and wnt8b mutants showed a decrease in the number of inhibitory interneurons in the optic tectum of larval animals. Furthermore  RNAseq revealed convergent transcriptional abnormalities between mutant lines  consistent with their developmental defects and hyperexcitable phenotypes. These zebrafish models provide strongest experimental evidence supporting the role of ARFGEF1  KCND2  and WNT8B in human epilepsy and further demonstrate the utility of this model system for evaluating candidate human epilepsy genes.", null, null, null, null, "Wnt8b Het3", null, "strain:Casper|age:5dpf|collection date:2022 12 09|geo loc name:USA: Boston  MA|sex:not applicable|tissue:Head|genotype:Wnt8b Het3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio Rerio: pools of isolated larval head", "Wnt8b Het3", "Wnt8b Het3", "Three replicate larvae from Het in crosses  aged 5 dpf  were sacrificed on ice and stored in RNAlater Stabilization Solution according to product specifications. Larval heads were isolated from bodies  which were used for Sanger sequencing for genotyping. Heads were pooled in groups of 20 25 according to genotype and RNA was isolated using a Qiagen RNeasy plus kit according to specifications. QC was performed via Agilent 2100 bioanalyzer  and only samples with an RNA integrity number value of >5.8 were used for library preparation.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "SINGLE", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP506997", null, null, "Wnt8b_Het3.fq.gz", "fastq", 4357330950.0, 29048873.0, "Wnt8b Het3.fq.gz", "0:150", "A:1179652667;C:1002955689;G:1008147493;T:1166494534;N:80567", 150, null, null, null, 1179652667, 1002955689, 1008147493, 1166494534, 80567, "SRX24520251", "SRS21267940", "SRA1863814", "Boston Childrens Hospital|Neurobiology", "Boston Childrens Hospital", null, null, null, null, null, null, null, null, null, null, null, "B", null, "usable mapping rate", "illumina", "novaseq_era", "unknown", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-05-10", "Larval", "Larval", "Head", "Nervous System"], [32068, "SRR29007557", "SRX24534635", "SRS21280318", "SRP507312", "PRJNA1111077", "Zebrafish models of candidate human epilepsy associated genes provide evidence of hyperexcitability", "PRJNA1111077", "Other", "Hundreds of novel candidate human epilepsy associated genes have been identified thanks to advancements in next generation sequencing and large genome wide association studies  but establishing genetic etiology requires functional validation. We generated a list of >2200 candidate epilepsy associated genes  of which 48 were developed into stable loss of function zebrafish models. Of those 48  evidence of seizure like behavior was present in 5 arfgef1  kcnd2  kcnv1  ubr5  wnt8b. Further characterization provided evidence for epileptiform activity via electrophysiology in kcnd2 and wnt8b mutants. Additionally  arfgef1 and wnt8b mutants showed a decrease in the number of inhibitory interneurons in the optic tectum of larval animals. Further  RNA Seq revealed convergent transcriptional abnormalities between mutant lines  consistent with their developmental defects and hyperexcitable phenotypes. These zebrafish models provide strongest experimental evidence supporting the role of ARFGEF1  KCND2  and WNT8B in human epilepsy and further demonstrate the utility of this model system for evaluating candidate human epilepsy genes.", null, null, null, null, "Kcnd2 Wt 1", null, "strain:Casper|age:5dpf|collection date:2022 12 09|geo loc name:USA: Boston  MA|sex:not applicable|tissue:Head|genotype:Kcnd2 Wt1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio Rerio: pools of isolated larval head", "Kcnd2 Wt 1", "Kcnd2 Wt 1", "Three replicate larvae from Het in crosses  aged 5 dpf  were sacrificed on ice and stored in RNAlater Stabilization Solution according to product specifications. Larval heads were isolated from bodies  which were used for Sanger sequencing for genotyping. Heads were pooled in groups of 20 25 according to genotype and RNA was isolated using a Qiagen RNeasy plus kit according to specifications. QC was performed via Agilent 2100 bioanalyzer  and only samples with an RNA integrity number value of >5.8 were used for library preparation.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP507312", null, null, "Kcnd2_Wt_1_1.fq.gz Kcnd2_Wt_1_2.fq.gz", "fastq fastq", 6772910100.0, 22576367.0, "Kcnd2 Wt 1 1.fq.gz", "0:150 1:150", "A:1933789152;C:1464135438;G:1474439143;T:1900463092;N:83275", 150, 150, null, null, 1933789152, 1464135438, 1474439143, 1900463092, 83275, "SRX24534635", "SRS21280318", "SRA1864976", "Boston Childrens Hospital|Neurobiology", "Boston Childrens Hospital", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-05-13", "Larval", "Larval", "Head", "Nervous System"], [32069, "SRR29007558", "SRX24534634", "SRS21280317", "SRP507312", "PRJNA1111077", "Zebrafish models of candidate human epilepsy associated genes provide evidence of hyperexcitability", "PRJNA1111077", "Other", "Hundreds of novel candidate human epilepsy associated genes have been identified thanks to advancements in next generation sequencing and large genome wide association studies  but establishing genetic etiology requires functional validation. We generated a list of >2200 candidate epilepsy associated genes  of which 48 were developed into stable loss of function zebrafish models. Of those 48  evidence of seizure like behavior was present in 5 arfgef1  kcnd2  kcnv1  ubr5  wnt8b. Further characterization provided evidence for epileptiform activity via electrophysiology in kcnd2 and wnt8b mutants. Additionally  arfgef1 and wnt8b mutants showed a decrease in the number of inhibitory interneurons in the optic tectum of larval animals. Further  RNA Seq revealed convergent transcriptional abnormalities between mutant lines  consistent with their developmental defects and hyperexcitable phenotypes. These zebrafish models provide strongest experimental evidence supporting the role of ARFGEF1  KCND2  and WNT8B in human epilepsy and further demonstrate the utility of this model system for evaluating candidate human epilepsy genes.", null, null, null, null, "Arfgef1 Hom 3", null, "strain:Casper|age:5dpf|collection date:2022 12 09|geo loc name:USA: Boston  MA|sex:not applicable|tissue:Head|genotype:Arfgef1 Hom3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio Rerio: pools of isolated larval head", "Arfgef1 Hom 3", "Arfgef1 Hom 3", "Three replicate larvae from Het in crosses  aged 5 dpf  were sacrificed on ice and stored in RNAlater Stabilization Solution according to product specifications. Larval heads were isolated from bodies  which were used for Sanger sequencing for genotyping. Heads were pooled in groups of 20 25 according to genotype and RNA was isolated using a Qiagen RNeasy plus kit according to specifications. QC was performed via Agilent 2100 bioanalyzer  and only samples with an RNA integrity number value of >5.8 were used for library preparation.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP507312", null, null, "Arfgef1_Hom_3_1.fq.gz Arfgef1_Hom_3_2.fq.gz", "fastq fastq", 7294731000.0, 24315770.0, "Arfgef1 Hom 3 1.fq.gz", "0:150 1:150", "A:1847018467;C:1809604703;G:1818278888;T:1819707786;N:121156", 150, 150, null, null, 1847018467, 1809604703, 1818278888, 1819707786, 121156, "SRX24534634", "SRS21280317", "SRA1864976", "Boston Childrens Hospital|Neurobiology", "Boston Childrens Hospital", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-05-13", "Larval", "Larval", "Head", "Nervous System"], [32070, "SRR29007559", "SRX24534633", "SRS21280316", "SRP507312", "PRJNA1111077", "Zebrafish models of candidate human epilepsy associated genes provide evidence of hyperexcitability", "PRJNA1111077", "Other", "Hundreds of novel candidate human epilepsy associated genes have been identified thanks to advancements in next generation sequencing and large genome wide association studies  but establishing genetic etiology requires functional validation. We generated a list of >2200 candidate epilepsy associated genes  of which 48 were developed into stable loss of function zebrafish models. Of those 48  evidence of seizure like behavior was present in 5 arfgef1  kcnd2  kcnv1  ubr5  wnt8b. Further characterization provided evidence for epileptiform activity via electrophysiology in kcnd2 and wnt8b mutants. Additionally  arfgef1 and wnt8b mutants showed a decrease in the number of inhibitory interneurons in the optic tectum of larval animals. Further  RNA Seq revealed convergent transcriptional abnormalities between mutant lines  consistent with their developmental defects and hyperexcitable phenotypes. These zebrafish models provide strongest experimental evidence supporting the role of ARFGEF1  KCND2  and WNT8B in human epilepsy and further demonstrate the utility of this model system for evaluating candidate human epilepsy genes.", null, null, null, null, "Arfgef1 Hom 2", null, "strain:Casper|age:5dpf|collection date:2022 12 09|geo loc name:USA: Boston  MA|sex:not applicable|tissue:Head|genotype:Arfgef1 Hom2|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio Rerio: pools of isolated larval head", "Arfgef1 Hom 2", "Arfgef1 Hom 2", "Three replicate larvae from Het in crosses  aged 5 dpf  were sacrificed on ice and stored in RNAlater Stabilization Solution according to product specifications. Larval heads were isolated from bodies  which were used for Sanger sequencing for genotyping. Heads were pooled in groups of 20 25 according to genotype and RNA was isolated using a Qiagen RNeasy plus kit according to specifications. QC was performed via Agilent 2100 bioanalyzer  and only samples with an RNA integrity number value of >5.8 were used for library preparation.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP507312", null, null, "Arfgef1_Hom_2_1.fq.gz Arfgef1_Hom_2_2.fq.gz", "fastq fastq", 6607825200.0, 22026084.0, "Arfgef1 Hom 2 1.fq.gz", "0:150 1:150", "A:1694417387;C:1616938849;G:1625276704;T:1671046414;N:145846", 150, 150, null, null, 1694417387, 1616938849, 1625276704, 1671046414, 145846, "SRX24534633", "SRS21280316", "SRA1864976", "Boston Childrens Hospital|Neurobiology", "Boston Childrens Hospital", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-05-13", "Larval", "Larval", "Head", "Nervous System"], [32071, "SRR29007560", "SRX24534632", "SRS21280315", "SRP507312", "PRJNA1111077", "Zebrafish models of candidate human epilepsy associated genes provide evidence of hyperexcitability", "PRJNA1111077", "Other", "Hundreds of novel candidate human epilepsy associated genes have been identified thanks to advancements in next generation sequencing and large genome wide association studies  but establishing genetic etiology requires functional validation. We generated a list of >2200 candidate epilepsy associated genes  of which 48 were developed into stable loss of function zebrafish models. Of those 48  evidence of seizure like behavior was present in 5 arfgef1  kcnd2  kcnv1  ubr5  wnt8b. Further characterization provided evidence for epileptiform activity via electrophysiology in kcnd2 and wnt8b mutants. Additionally  arfgef1 and wnt8b mutants showed a decrease in the number of inhibitory interneurons in the optic tectum of larval animals. Further  RNA Seq revealed convergent transcriptional abnormalities between mutant lines  consistent with their developmental defects and hyperexcitable phenotypes. These zebrafish models provide strongest experimental evidence supporting the role of ARFGEF1  KCND2  and WNT8B in human epilepsy and further demonstrate the utility of this model system for evaluating candidate human epilepsy genes.", null, null, null, null, "Arfgef1 Hom 1", null, "strain:Casper|age:5dpf|collection date:2022 12 09|geo loc name:USA: Boston  MA|sex:not applicable|tissue:Head|genotype:Arfgef1 Hom1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio Rerio: pools of isolated larval head", "Arfgef1 Hom 1", "Arfgef1 Hom 1", "Three replicate larvae from Het in crosses  aged 5 dpf  were sacrificed on ice and stored in RNAlater Stabilization Solution according to product specifications. Larval heads were isolated from bodies  which were used for Sanger sequencing for genotyping. Heads were pooled in groups of 20 25 according to genotype and RNA was isolated using a Qiagen RNeasy plus kit according to specifications. QC was performed via Agilent 2100 bioanalyzer  and only samples with an RNA integrity number value of >5.8 were used for library preparation.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP507312", null, null, "Arfgef1_Hom_1_1.fq.gz Arfgef1_Hom_1_2.fq.gz", "fastq fastq", 6777738600.0, 22592462.0, "Arfgef1 Hom 1 1.fq.gz", "0:150 1:150", "A:1739306802;C:1658195023;G:1665806667;T:1714318423;N:111685", 150, 150, null, null, 1739306802, 1658195023, 1665806667, 1714318423, 111685, "SRX24534632", "SRS21280315", "SRA1864976", "Boston Childrens Hospital|Neurobiology", "Boston Childrens Hospital", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-05-13", "Larval", "Larval", "Head", "Nervous System"], [32072, "SRR29007561", "SRX24534631", "SRS21280314", "SRP507312", "PRJNA1111077", "Zebrafish models of candidate human epilepsy associated genes provide evidence of hyperexcitability", "PRJNA1111077", "Other", "Hundreds of novel candidate human epilepsy associated genes have been identified thanks to advancements in next generation sequencing and large genome wide association studies  but establishing genetic etiology requires functional validation. We generated a list of >2200 candidate epilepsy associated genes  of which 48 were developed into stable loss of function zebrafish models. Of those 48  evidence of seizure like behavior was present in 5 arfgef1  kcnd2  kcnv1  ubr5  wnt8b. Further characterization provided evidence for epileptiform activity via electrophysiology in kcnd2 and wnt8b mutants. Additionally  arfgef1 and wnt8b mutants showed a decrease in the number of inhibitory interneurons in the optic tectum of larval animals. Further  RNA Seq revealed convergent transcriptional abnormalities between mutant lines  consistent with their developmental defects and hyperexcitable phenotypes. These zebrafish models provide strongest experimental evidence supporting the role of ARFGEF1  KCND2  and WNT8B in human epilepsy and further demonstrate the utility of this model system for evaluating candidate human epilepsy genes.", null, null, null, null, "Arfgef1 Het 3", null, "strain:Casper|age:5dpf|collection date:2022 12 09|geo loc name:USA: Boston  MA|sex:not applicable|tissue:Head|genotype:Arfgef1 Het3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio Rerio: pools of isolated larval head", "Arfgef1 Het 3", "Arfgef1 Het 3", "Three replicate larvae from Het in crosses  aged 5 dpf  were sacrificed on ice and stored in RNAlater Stabilization Solution according to product specifications. Larval heads were isolated from bodies  which were used for Sanger sequencing for genotyping. Heads were pooled in groups of 20 25 according to genotype and RNA was isolated using a Qiagen RNeasy plus kit according to specifications. QC was performed via Agilent 2100 bioanalyzer  and only samples with an RNA integrity number value of >5.8 were used for library preparation.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP507312", null, null, "Arfgef1_Het_3_1.fq.gz Arfgef1_Het_3_2.fq.gz", "fastq fastq", 6739993500.0, 22466645.0, "Arfgef1 Het 3 1.fq.gz", "0:150 1:150", "A:1725253563;C:1652471358;G:1662793828;T:1699392429;N:82322", 150, 150, null, null, 1725253563, 1652471358, 1662793828, 1699392429, 82322, "SRX24534631", "SRS21280314", "SRA1864976", "Boston Childrens Hospital|Neurobiology", "Boston Childrens Hospital", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-05-13", "Larval", "Larval", "Head", "Nervous System"], [32073, "SRR29007562", "SRX24534630", "SRS21280313", "SRP507312", "PRJNA1111077", "Zebrafish models of candidate human epilepsy associated genes provide evidence of hyperexcitability", "PRJNA1111077", "Other", "Hundreds of novel candidate human epilepsy associated genes have been identified thanks to advancements in next generation sequencing and large genome wide association studies  but establishing genetic etiology requires functional validation. We generated a list of >2200 candidate epilepsy associated genes  of which 48 were developed into stable loss of function zebrafish models. Of those 48  evidence of seizure like behavior was present in 5 arfgef1  kcnd2  kcnv1  ubr5  wnt8b. Further characterization provided evidence for epileptiform activity via electrophysiology in kcnd2 and wnt8b mutants. Additionally  arfgef1 and wnt8b mutants showed a decrease in the number of inhibitory interneurons in the optic tectum of larval animals. Further  RNA Seq revealed convergent transcriptional abnormalities between mutant lines  consistent with their developmental defects and hyperexcitable phenotypes. These zebrafish models provide strongest experimental evidence supporting the role of ARFGEF1  KCND2  and WNT8B in human epilepsy and further demonstrate the utility of this model system for evaluating candidate human epilepsy genes.", null, null, null, null, "Arfgef1 Het 2", null, "strain:Casper|age:5dpf|collection date:2022 12 09|geo loc name:USA: Boston  MA|sex:not applicable|tissue:Head|genotype:Arfgef1 Het2|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio Rerio: pools of isolated larval head", "Arfgef1 Het 2", "Arfgef1 Het 2", "Three replicate larvae from Het in crosses  aged 5 dpf  were sacrificed on ice and stored in RNAlater Stabilization Solution according to product specifications. Larval heads were isolated from bodies  which were used for Sanger sequencing for genotyping. Heads were pooled in groups of 20 25 according to genotype and RNA was isolated using a Qiagen RNeasy plus kit according to specifications. QC was performed via Agilent 2100 bioanalyzer  and only samples with an RNA integrity number value of >5.8 were used for library preparation.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP507312", null, null, "Arfgef1_Het_2_1.fq.gz Arfgef1_Het_2_2.fq.gz", "fastq fastq", 9510813000.0, 31702710.0, "Arfgef1 Het 2 1.fq.gz", "0:150 1:150", "A:2509621883;C:2259671963;G:2271899998;T:2469380560;N:238596", 150, 150, null, null, 2509621883, 2259671963, 2271899998, 2469380560, 238596, "SRX24534630", "SRS21280313", "SRA1864976", "Boston Childrens Hospital|Neurobiology", "Boston Childrens Hospital", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-05-13", "Larval", "Larval", "Head", "Nervous System"], [32074, "SRR29007563", "SRX24534629", "SRS21280312", "SRP507312", "PRJNA1111077", "Zebrafish models of candidate human epilepsy associated genes provide evidence of hyperexcitability", "PRJNA1111077", "Other", "Hundreds of novel candidate human epilepsy associated genes have been identified thanks to advancements in next generation sequencing and large genome wide association studies  but establishing genetic etiology requires functional validation. We generated a list of >2200 candidate epilepsy associated genes  of which 48 were developed into stable loss of function zebrafish models. Of those 48  evidence of seizure like behavior was present in 5 arfgef1  kcnd2  kcnv1  ubr5  wnt8b. Further characterization provided evidence for epileptiform activity via electrophysiology in kcnd2 and wnt8b mutants. Additionally  arfgef1 and wnt8b mutants showed a decrease in the number of inhibitory interneurons in the optic tectum of larval animals. Further  RNA Seq revealed convergent transcriptional abnormalities between mutant lines  consistent with their developmental defects and hyperexcitable phenotypes. These zebrafish models provide strongest experimental evidence supporting the role of ARFGEF1  KCND2  and WNT8B in human epilepsy and further demonstrate the utility of this model system for evaluating candidate human epilepsy genes.", null, null, null, null, "Arfgef1 Het 1", null, "strain:Casper|age:5dpf|collection date:2022 12 09|geo loc name:USA: Boston  MA|sex:not applicable|tissue:Head|genotype:Arfgef1 Het1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio Rerio: pools of isolated larval head", "Arfgef1 Het 1", "Arfgef1 Het 1", "Three replicate larvae from Het in crosses  aged 5 dpf  were sacrificed on ice and stored in RNAlater Stabilization Solution according to product specifications. Larval heads were isolated from bodies  which were used for Sanger sequencing for genotyping. Heads were pooled in groups of 20 25 according to genotype and RNA was isolated using a Qiagen RNeasy plus kit according to specifications. QC was performed via Agilent 2100 bioanalyzer  and only samples with an RNA integrity number value of >5.8 were used for library preparation.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP507312", null, null, "Arfgef1_Het_1_1.fq.gz Arfgef1_Het_1_2.fq.gz", "fastq fastq", 6887369100.0, 22957897.0, "Arfgef1 Het 1 1.fq.gz", "0:150 1:150", "A:1766549896;C:1685533018;G:1694949682;T:1740214059;N:122445", 150, 150, null, null, 1766549896, 1685533018, 1694949682, 1740214059, 122445, "SRX24534629", "SRS21280312", "SRA1864976", "Boston Childrens Hospital|Neurobiology", "Boston Childrens Hospital", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-05-13", "Larval", "Larval", "Head", "Nervous System"], [32075, "SRR29007564", "SRX24534628", "SRS21280311", "SRP507312", "PRJNA1111077", "Zebrafish models of candidate human epilepsy associated genes provide evidence of hyperexcitability", "PRJNA1111077", "Other", "Hundreds of novel candidate human epilepsy associated genes have been identified thanks to advancements in next generation sequencing and large genome wide association studies  but establishing genetic etiology requires functional validation. We generated a list of >2200 candidate epilepsy associated genes  of which 48 were developed into stable loss of function zebrafish models. Of those 48  evidence of seizure like behavior was present in 5 arfgef1  kcnd2  kcnv1  ubr5  wnt8b. Further characterization provided evidence for epileptiform activity via electrophysiology in kcnd2 and wnt8b mutants. Additionally  arfgef1 and wnt8b mutants showed a decrease in the number of inhibitory interneurons in the optic tectum of larval animals. Further  RNA Seq revealed convergent transcriptional abnormalities between mutant lines  consistent with their developmental defects and hyperexcitable phenotypes. These zebrafish models provide strongest experimental evidence supporting the role of ARFGEF1  KCND2  and WNT8B in human epilepsy and further demonstrate the utility of this model system for evaluating candidate human epilepsy genes.", null, null, null, null, "Wnt8b Hom 3", null, "strain:Casper|age:5dpf|collection date:2022 12 09|geo loc name:USA: Boston  MA|sex:not applicable|tissue:Head|genotype:Wnt8b Hom3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio Rerio: pools of isolated larval head", "Wnt8b Hom 3", "Wnt8b Hom 3", "Three replicate larvae from Het in crosses  aged 5 dpf  were sacrificed on ice and stored in RNAlater Stabilization Solution according to product specifications. Larval heads were isolated from bodies  which were used for Sanger sequencing for genotyping. Heads were pooled in groups of 20 25 according to genotype and RNA was isolated using a Qiagen RNeasy plus kit according to specifications. QC was performed via Agilent 2100 bioanalyzer  and only samples with an RNA integrity number value of >5.8 were used for library preparation.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP507312", null, null, "Wnt8b_Hom_3_1.fq.gz Wnt8b_Hom_3_2.fq.gz", "fastq fastq", 10567857900.0, 35226193.0, "Wnt8b Hom 3 1.fq.gz", "0:150 1:150", "A:2775044850;C:2525957688;G:2527072827;T:2739479844;N:302691", 150, 150, null, null, 2775044850, 2525957688, 2527072827, 2739479844, 302691, "SRX24534628", "SRS21280311", "SRA1864976", "Boston Childrens Hospital|Neurobiology", "Boston Childrens Hospital", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-05-13", "Larval", "Larval", "Head", "Nervous System"], [32076, "SRR29007565", "SRX24534627", "SRS21280310", "SRP507312", "PRJNA1111077", "Zebrafish models of candidate human epilepsy associated genes provide evidence of hyperexcitability", "PRJNA1111077", "Other", "Hundreds of novel candidate human epilepsy associated genes have been identified thanks to advancements in next generation sequencing and large genome wide association studies  but establishing genetic etiology requires functional validation. We generated a list of >2200 candidate epilepsy associated genes  of which 48 were developed into stable loss of function zebrafish models. Of those 48  evidence of seizure like behavior was present in 5 arfgef1  kcnd2  kcnv1  ubr5  wnt8b. Further characterization provided evidence for epileptiform activity via electrophysiology in kcnd2 and wnt8b mutants. Additionally  arfgef1 and wnt8b mutants showed a decrease in the number of inhibitory interneurons in the optic tectum of larval animals. Further  RNA Seq revealed convergent transcriptional abnormalities between mutant lines  consistent with their developmental defects and hyperexcitable phenotypes. These zebrafish models provide strongest experimental evidence supporting the role of ARFGEF1  KCND2  and WNT8B in human epilepsy and further demonstrate the utility of this model system for evaluating candidate human epilepsy genes.", null, null, null, null, "Wnt8b Hom 2", null, "strain:Casper|age:5dpf|collection date:2022 12 09|geo loc name:USA: Boston  MA|sex:not applicable|tissue:Head|genotype:Wnt8b Hom2|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio Rerio: pools of isolated larval head", "Wnt8b Hom 2", "Wnt8b Hom 2", "Three replicate larvae from Het in crosses  aged 5 dpf  were sacrificed on ice and stored in RNAlater Stabilization Solution according to product specifications. Larval heads were isolated from bodies  which were used for Sanger sequencing for genotyping. Heads were pooled in groups of 20 25 according to genotype and RNA was isolated using a Qiagen RNeasy plus kit according to specifications. QC was performed via Agilent 2100 bioanalyzer  and only samples with an RNA integrity number value of >5.8 were used for library preparation.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP507312", null, null, "Wnt8b_Hom_2_1.fq.gz Wnt8b_Hom_2_2.fq.gz", "fastq fastq", 7666806600.0, 25556022.0, "Wnt8b Hom 2 1.fq.gz", "0:150 1:150", "A:2029345938;C:1820579149;G:1819606043;T:1996786660;N:488810", 150, 150, null, null, 2029345938, 1820579149, 1819606043, 1996786660, 488810, "SRX24534627", "SRS21280310", "SRA1864976", "Boston Childrens Hospital|Neurobiology", "Boston Childrens Hospital", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-05-13", "Larval", "Larval", "Head", "Nervous System"], [32077, "SRR29007566", "SRX24534626", "SRS21280309", "SRP507312", "PRJNA1111077", "Zebrafish models of candidate human epilepsy associated genes provide evidence of hyperexcitability", "PRJNA1111077", "Other", "Hundreds of novel candidate human epilepsy associated genes have been identified thanks to advancements in next generation sequencing and large genome wide association studies  but establishing genetic etiology requires functional validation. We generated a list of >2200 candidate epilepsy associated genes  of which 48 were developed into stable loss of function zebrafish models. Of those 48  evidence of seizure like behavior was present in 5 arfgef1  kcnd2  kcnv1  ubr5  wnt8b. Further characterization provided evidence for epileptiform activity via electrophysiology in kcnd2 and wnt8b mutants. Additionally  arfgef1 and wnt8b mutants showed a decrease in the number of inhibitory interneurons in the optic tectum of larval animals. Further  RNA Seq revealed convergent transcriptional abnormalities between mutant lines  consistent with their developmental defects and hyperexcitable phenotypes. These zebrafish models provide strongest experimental evidence supporting the role of ARFGEF1  KCND2  and WNT8B in human epilepsy and further demonstrate the utility of this model system for evaluating candidate human epilepsy genes.", null, null, null, null, "Wnt8b Hom 1", null, "strain:Casper|age:5dpf|collection date:2022 12 09|geo loc name:USA: Boston  MA|sex:not applicable|tissue:Head|genotype:Wnt8b Hom1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio Rerio: pools of isolated larval head", "Wnt8b Hom 1", "Wnt8b Hom 1", "Three replicate larvae from Het in crosses  aged 5 dpf  were sacrificed on ice and stored in RNAlater Stabilization Solution according to product specifications. Larval heads were isolated from bodies  which were used for Sanger sequencing for genotyping. Heads were pooled in groups of 20 25 according to genotype and RNA was isolated using a Qiagen RNeasy plus kit according to specifications. QC was performed via Agilent 2100 bioanalyzer  and only samples with an RNA integrity number value of >5.8 were used for library preparation.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP507312", null, null, "Wnt8b_Hom_1_1.fq.gz Wnt8b_Hom_1_2.fq.gz", "fastq fastq", 6514544400.0, 21715148.0, "Wnt8b Hom 1 1.fq.gz", "0:150 1:150", "A:1711943699;C:1556010812;G:1555687818;T:1690731439;N:170632", 150, 150, null, null, 1711943699, 1556010812, 1555687818, 1690731439, 170632, "SRX24534626", "SRS21280309", "SRA1864976", "Boston Childrens Hospital|Neurobiology", "Boston Childrens Hospital", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-05-13", "Larval", "Larval", "Head", "Nervous System"], [32078, "SRR29007567", "SRX24534625", "SRS21280308", "SRP507312", "PRJNA1111077", "Zebrafish models of candidate human epilepsy associated genes provide evidence of hyperexcitability", "PRJNA1111077", "Other", "Hundreds of novel candidate human epilepsy associated genes have been identified thanks to advancements in next generation sequencing and large genome wide association studies  but establishing genetic etiology requires functional validation. We generated a list of >2200 candidate epilepsy associated genes  of which 48 were developed into stable loss of function zebrafish models. Of those 48  evidence of seizure like behavior was present in 5 arfgef1  kcnd2  kcnv1  ubr5  wnt8b. Further characterization provided evidence for epileptiform activity via electrophysiology in kcnd2 and wnt8b mutants. Additionally  arfgef1 and wnt8b mutants showed a decrease in the number of inhibitory interneurons in the optic tectum of larval animals. Further  RNA Seq revealed convergent transcriptional abnormalities between mutant lines  consistent with their developmental defects and hyperexcitable phenotypes. These zebrafish models provide strongest experimental evidence supporting the role of ARFGEF1  KCND2  and WNT8B in human epilepsy and further demonstrate the utility of this model system for evaluating candidate human epilepsy genes.", null, null, null, null, "Wnt8b Het 3", null, "strain:Casper|age:5dpf|collection date:2022 12 09|geo loc name:USA: Boston  MA|sex:not applicable|tissue:Head|genotype:Wnt8b Het3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio Rerio: pools of isolated larval head", "Wnt8b Het 3", "Wnt8b Het 3", "Three replicate larvae from Het in crosses  aged 5 dpf  were sacrificed on ice and stored in RNAlater Stabilization Solution according to product specifications. Larval heads were isolated from bodies  which were used for Sanger sequencing for genotyping. Heads were pooled in groups of 20 25 according to genotype and RNA was isolated using a Qiagen RNeasy plus kit according to specifications. QC was performed via Agilent 2100 bioanalyzer  and only samples with an RNA integrity number value of >5.8 were used for library preparation.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP507312", null, null, "Wnt8b_Het_3_1.fq.gz Wnt8b_Het_3_2.fq.gz", "fastq fastq", 8714661900.0, 29048873.0, "Wnt8b Het 3 1.fq.gz", "0:150 1:150", "A:2360994905;C:2008091028;G:2013456743;T:2332006781;N:112443", 150, 150, null, null, 2360994905, 2008091028, 2013456743, 2332006781, 112443, "SRX24534625", "SRS21280308", "SRA1864976", "Boston Childrens Hospital|Neurobiology", "Boston Childrens Hospital", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-05-13", "Larval", "Larval", "Head", "Nervous System"], [32079, "SRR29007568", "SRX24534624", "SRS21280307", "SRP507312", "PRJNA1111077", "Zebrafish models of candidate human epilepsy associated genes provide evidence of hyperexcitability", "PRJNA1111077", "Other", "Hundreds of novel candidate human epilepsy associated genes have been identified thanks to advancements in next generation sequencing and large genome wide association studies  but establishing genetic etiology requires functional validation. We generated a list of >2200 candidate epilepsy associated genes  of which 48 were developed into stable loss of function zebrafish models. Of those 48  evidence of seizure like behavior was present in 5 arfgef1  kcnd2  kcnv1  ubr5  wnt8b. Further characterization provided evidence for epileptiform activity via electrophysiology in kcnd2 and wnt8b mutants. Additionally  arfgef1 and wnt8b mutants showed a decrease in the number of inhibitory interneurons in the optic tectum of larval animals. Further  RNA Seq revealed convergent transcriptional abnormalities between mutant lines  consistent with their developmental defects and hyperexcitable phenotypes. These zebrafish models provide strongest experimental evidence supporting the role of ARFGEF1  KCND2  and WNT8B in human epilepsy and further demonstrate the utility of this model system for evaluating candidate human epilepsy genes.", null, null, null, null, "Wnt8b Het 2", null, "strain:Casper|age:5dpf|collection date:2022 12 09|geo loc name:USA: Boston  MA|sex:not applicable|tissue:Head|genotype:Wnt8b Het2|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio Rerio: pools of isolated larval head", "Wnt8b Het 2", "Wnt8b Het 2", "Three replicate larvae from Het in crosses  aged 5 dpf  were sacrificed on ice and stored in RNAlater Stabilization Solution according to product specifications. Larval heads were isolated from bodies  which were used for Sanger sequencing for genotyping. Heads were pooled in groups of 20 25 according to genotype and RNA was isolated using a Qiagen RNeasy plus kit according to specifications. QC was performed via Agilent 2100 bioanalyzer  and only samples with an RNA integrity number value of >5.8 were used for library preparation.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP507312", null, null, "Wnt8b_Het_2_1.fq.gz Wnt8b_Het_2_2.fq.gz", "fastq fastq", 8370112800.0, 27900376.0, "Wnt8b Het 2 1.fq.gz", "0:150 1:150", "A:2275496039;C:1920819728;G:1927597917;T:2246091711;N:107405", 150, 150, null, null, 2275496039, 1920819728, 1927597917, 2246091711, 107405, "SRX24534624", "SRS21280307", "SRA1864976", "Boston Childrens Hospital|Neurobiology", "Boston Childrens Hospital", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-05-13", "Larval", "Larval", "Head", "Nervous System"], [32080, "SRR29007569", "SRX24534623", "SRS21280306", "SRP507312", "PRJNA1111077", "Zebrafish models of candidate human epilepsy associated genes provide evidence of hyperexcitability", "PRJNA1111077", "Other", "Hundreds of novel candidate human epilepsy associated genes have been identified thanks to advancements in next generation sequencing and large genome wide association studies  but establishing genetic etiology requires functional validation. We generated a list of >2200 candidate epilepsy associated genes  of which 48 were developed into stable loss of function zebrafish models. Of those 48  evidence of seizure like behavior was present in 5 arfgef1  kcnd2  kcnv1  ubr5  wnt8b. Further characterization provided evidence for epileptiform activity via electrophysiology in kcnd2 and wnt8b mutants. Additionally  arfgef1 and wnt8b mutants showed a decrease in the number of inhibitory interneurons in the optic tectum of larval animals. Further  RNA Seq revealed convergent transcriptional abnormalities between mutant lines  consistent with their developmental defects and hyperexcitable phenotypes. These zebrafish models provide strongest experimental evidence supporting the role of ARFGEF1  KCND2  and WNT8B in human epilepsy and further demonstrate the utility of this model system for evaluating candidate human epilepsy genes.", null, null, null, null, "Wnt8b Het 1", null, "strain:Casper|age:5dpf|collection date:2022 12 09|geo loc name:USA: Boston  MA|sex:not applicable|tissue:Head|genotype:Wnt8b Het1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio Rerio: pools of isolated larval head", "Wnt8b Het 1", "Wnt8b Het 1", "Three replicate larvae from Het in crosses  aged 5 dpf  were sacrificed on ice and stored in RNAlater Stabilization Solution according to product specifications. Larval heads were isolated from bodies  which were used for Sanger sequencing for genotyping. Heads were pooled in groups of 20 25 according to genotype and RNA was isolated using a Qiagen RNeasy plus kit according to specifications. QC was performed via Agilent 2100 bioanalyzer  and only samples with an RNA integrity number value of >5.8 were used for library preparation.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP507312", null, null, "Wnt8b_Het_1_1.fq.gz Wnt8b_Het_1_2.fq.gz", "fastq fastq", 7614189000.0, 25380630.0, "Wnt8b Het 1 1.fq.gz", "0:150 1:150", "A:2059784093;C:1755740426;G:1761242316;T:2037325656;N:96509", 150, 150, null, null, 2059784093, 1755740426, 1761242316, 2037325656, 96509, "SRX24534623", "SRS21280306", "SRA1864976", "Boston Childrens Hospital|Neurobiology", "Boston Childrens Hospital", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-05-13", "Larval", "Larval", "Head", "Nervous System"], [32081, "SRR29007570", "SRX24534622", "SRS21280305", "SRP507312", "PRJNA1111077", "Zebrafish models of candidate human epilepsy associated genes provide evidence of hyperexcitability", "PRJNA1111077", "Other", "Hundreds of novel candidate human epilepsy associated genes have been identified thanks to advancements in next generation sequencing and large genome wide association studies  but establishing genetic etiology requires functional validation. We generated a list of >2200 candidate epilepsy associated genes  of which 48 were developed into stable loss of function zebrafish models. Of those 48  evidence of seizure like behavior was present in 5 arfgef1  kcnd2  kcnv1  ubr5  wnt8b. Further characterization provided evidence for epileptiform activity via electrophysiology in kcnd2 and wnt8b mutants. Additionally  arfgef1 and wnt8b mutants showed a decrease in the number of inhibitory interneurons in the optic tectum of larval animals. Further  RNA Seq revealed convergent transcriptional abnormalities between mutant lines  consistent with their developmental defects and hyperexcitable phenotypes. These zebrafish models provide strongest experimental evidence supporting the role of ARFGEF1  KCND2  and WNT8B in human epilepsy and further demonstrate the utility of this model system for evaluating candidate human epilepsy genes.", null, null, null, null, "Wnt8b Wt 3", null, "strain:Casper|age:5dpf|collection date:2022 12 09|geo loc name:USA: Boston  MA|sex:not applicable|tissue:Head|genotype:Wnt8b Wt3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio Rerio: pools of isolated larval head", "Wnt8b Wt 3", "Wnt8b Wt 3", "Three replicate larvae from Het in crosses  aged 5 dpf  were sacrificed on ice and stored in RNAlater Stabilization Solution according to product specifications. Larval heads were isolated from bodies  which were used for Sanger sequencing for genotyping. Heads were pooled in groups of 20 25 according to genotype and RNA was isolated using a Qiagen RNeasy plus kit according to specifications. QC was performed via Agilent 2100 bioanalyzer  and only samples with an RNA integrity number value of >5.8 were used for library preparation.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP507312", null, null, "Wnt8b_Wt_3_1.fq.gz Wnt8b_Wt_3_2.fq.gz", "fastq fastq", 6809737500.0, 22699125.0, "Wnt8b Wt 3 1.fq.gz", "0:150 1:150", "A:1869159615;C:1548130956;G:1547169482;T:1845192460;N:84987", 150, 150, null, null, 1869159615, 1548130956, 1547169482, 1845192460, 84987, "SRX24534622", "SRS21280305", "SRA1864976", "Boston Childrens Hospital|Neurobiology", "Boston Childrens Hospital", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-05-13", "Larval", "Larval", "Head", "Nervous System"], [32082, "SRR29007571", "SRX24534621", "SRS21280304", "SRP507312", "PRJNA1111077", "Zebrafish models of candidate human epilepsy associated genes provide evidence of hyperexcitability", "PRJNA1111077", "Other", "Hundreds of novel candidate human epilepsy associated genes have been identified thanks to advancements in next generation sequencing and large genome wide association studies  but establishing genetic etiology requires functional validation. We generated a list of >2200 candidate epilepsy associated genes  of which 48 were developed into stable loss of function zebrafish models. Of those 48  evidence of seizure like behavior was present in 5 arfgef1  kcnd2  kcnv1  ubr5  wnt8b. Further characterization provided evidence for epileptiform activity via electrophysiology in kcnd2 and wnt8b mutants. Additionally  arfgef1 and wnt8b mutants showed a decrease in the number of inhibitory interneurons in the optic tectum of larval animals. Further  RNA Seq revealed convergent transcriptional abnormalities between mutant lines  consistent with their developmental defects and hyperexcitable phenotypes. These zebrafish models provide strongest experimental evidence supporting the role of ARFGEF1  KCND2  and WNT8B in human epilepsy and further demonstrate the utility of this model system for evaluating candidate human epilepsy genes.", null, null, null, null, "Arfgef1 Wt 3", null, "strain:Casper|age:5dpf|collection date:2022 12 09|geo loc name:USA: Boston  MA|sex:not applicable|tissue:Head|genotype:Arfgef1 Wt3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio Rerio: pools of isolated larval head", "Arfgef1 Wt 3", "Arfgef1 Wt 3", "Three replicate larvae from Het in crosses  aged 5 dpf  were sacrificed on ice and stored in RNAlater Stabilization Solution according to product specifications. Larval heads were isolated from bodies  which were used for Sanger sequencing for genotyping. Heads were pooled in groups of 20 25 according to genotype and RNA was isolated using a Qiagen RNeasy plus kit according to specifications. QC was performed via Agilent 2100 bioanalyzer  and only samples with an RNA integrity number value of >5.8 were used for library preparation.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP507312", null, null, "Arfgef1_Wt_3_1.fq.gz Arfgef1_Wt_3_2.fq.gz", "fastq fastq", 6162364200.0, 20541214.0, "Arfgef1 Wt 3 1.fq.gz", "0:150 1:150", "A:1606427072;C:1484826935;G:1488745137;T:1582299135;N:65921", 150, 150, null, null, 1606427072, 1484826935, 1488745137, 1582299135, 65921, "SRX24534621", "SRS21280304", "SRA1864976", "Boston Childrens Hospital|Neurobiology", "Boston Childrens Hospital", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-05-13", "Larval", "Larval", "Head", "Nervous System"], [32083, "SRR29007572", "SRX24534620", "SRS21280303", "SRP507312", "PRJNA1111077", "Zebrafish models of candidate human epilepsy associated genes provide evidence of hyperexcitability", "PRJNA1111077", "Other", "Hundreds of novel candidate human epilepsy associated genes have been identified thanks to advancements in next generation sequencing and large genome wide association studies  but establishing genetic etiology requires functional validation. We generated a list of >2200 candidate epilepsy associated genes  of which 48 were developed into stable loss of function zebrafish models. Of those 48  evidence of seizure like behavior was present in 5 arfgef1  kcnd2  kcnv1  ubr5  wnt8b. Further characterization provided evidence for epileptiform activity via electrophysiology in kcnd2 and wnt8b mutants. Additionally  arfgef1 and wnt8b mutants showed a decrease in the number of inhibitory interneurons in the optic tectum of larval animals. Further  RNA Seq revealed convergent transcriptional abnormalities between mutant lines  consistent with their developmental defects and hyperexcitable phenotypes. These zebrafish models provide strongest experimental evidence supporting the role of ARFGEF1  KCND2  and WNT8B in human epilepsy and further demonstrate the utility of this model system for evaluating candidate human epilepsy genes.", null, null, null, null, "Wnt8b Wt 2", null, "strain:Casper|age:5dpf|collection date:2022 12 09|geo loc name:USA: Boston  MA|sex:not applicable|tissue:Head|genotype:Wnt8b Wt2|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio Rerio: pools of isolated larval head", "Wnt8b Wt 2", "Wnt8b Wt 2", "Three replicate larvae from Het in crosses  aged 5 dpf  were sacrificed on ice and stored in RNAlater Stabilization Solution according to product specifications. Larval heads were isolated from bodies  which were used for Sanger sequencing for genotyping. Heads were pooled in groups of 20 25 according to genotype and RNA was isolated using a Qiagen RNeasy plus kit according to specifications. QC was performed via Agilent 2100 bioanalyzer  and only samples with an RNA integrity number value of >5.8 were used for library preparation.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP507312", null, null, "Wnt8b_Wt_2_1.fq.gz Wnt8b_Wt_2_2.fq.gz", "fastq fastq", 6743670600.0, 22478902.0, "Wnt8b Wt 2 1.fq.gz", "0:150 1:150", "A:1852520280;C:1531023930;G:1536795067;T:1823243896;N:87427", 150, 150, null, null, 1852520280, 1531023930, 1536795067, 1823243896, 87427, "SRX24534620", "SRS21280303", "SRA1864976", "Boston Childrens Hospital|Neurobiology", "Boston Childrens Hospital", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-05-13", "Larval", "Larval", "Head", "Nervous System"], [32084, "SRR29007573", "SRX24534619", "SRS21280302", "SRP507312", "PRJNA1111077", "Zebrafish models of candidate human epilepsy associated genes provide evidence of hyperexcitability", "PRJNA1111077", "Other", "Hundreds of novel candidate human epilepsy associated genes have been identified thanks to advancements in next generation sequencing and large genome wide association studies  but establishing genetic etiology requires functional validation. We generated a list of >2200 candidate epilepsy associated genes  of which 48 were developed into stable loss of function zebrafish models. Of those 48  evidence of seizure like behavior was present in 5 arfgef1  kcnd2  kcnv1  ubr5  wnt8b. Further characterization provided evidence for epileptiform activity via electrophysiology in kcnd2 and wnt8b mutants. Additionally  arfgef1 and wnt8b mutants showed a decrease in the number of inhibitory interneurons in the optic tectum of larval animals. Further  RNA Seq revealed convergent transcriptional abnormalities between mutant lines  consistent with their developmental defects and hyperexcitable phenotypes. These zebrafish models provide strongest experimental evidence supporting the role of ARFGEF1  KCND2  and WNT8B in human epilepsy and further demonstrate the utility of this model system for evaluating candidate human epilepsy genes.", null, null, null, null, "Wnt8b Wt 1", null, "strain:Casper|age:5dpf|collection date:2022 12 09|geo loc name:USA: Boston  MA|sex:not applicable|tissue:Head|genotype:Wnt8b Wt1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio Rerio: pools of isolated larval head", "Wnt8b Wt 1", "Wnt8b Wt 1", "Three replicate larvae from Het in crosses  aged 5 dpf  were sacrificed on ice and stored in RNAlater Stabilization Solution according to product specifications. Larval heads were isolated from bodies  which were used for Sanger sequencing for genotyping. Heads were pooled in groups of 20 25 according to genotype and RNA was isolated using a Qiagen RNeasy plus kit according to specifications. QC was performed via Agilent 2100 bioanalyzer  and only samples with an RNA integrity number value of >5.8 were used for library preparation.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP507312", null, null, "Wnt8b_Wt_1_1.fq.gz Wnt8b_Wt_1_2.fq.gz", "fastq fastq", 6534620400.0, 21782068.0, "Wnt8b Wt 1 1.fq.gz", "0:150 1:150", "A:1783250074;C:1493088330;G:1497228073;T:1760957638;N:96285", 150, 150, null, null, 1783250074, 1493088330, 1497228073, 1760957638, 96285, "SRX24534619", "SRS21280302", "SRA1864976", "Boston Childrens Hospital|Neurobiology", "Boston Childrens Hospital", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-05-13", "Larval", "Larval", "Head", "Nervous System"], [32085, "SRR29007574", "SRX24534618", "SRS21280301", "SRP507312", "PRJNA1111077", "Zebrafish models of candidate human epilepsy associated genes provide evidence of hyperexcitability", "PRJNA1111077", "Other", "Hundreds of novel candidate human epilepsy associated genes have been identified thanks to advancements in next generation sequencing and large genome wide association studies  but establishing genetic etiology requires functional validation. We generated a list of >2200 candidate epilepsy associated genes  of which 48 were developed into stable loss of function zebrafish models. Of those 48  evidence of seizure like behavior was present in 5 arfgef1  kcnd2  kcnv1  ubr5  wnt8b. Further characterization provided evidence for epileptiform activity via electrophysiology in kcnd2 and wnt8b mutants. Additionally  arfgef1 and wnt8b mutants showed a decrease in the number of inhibitory interneurons in the optic tectum of larval animals. Further  RNA Seq revealed convergent transcriptional abnormalities between mutant lines  consistent with their developmental defects and hyperexcitable phenotypes. These zebrafish models provide strongest experimental evidence supporting the role of ARFGEF1  KCND2  and WNT8B in human epilepsy and further demonstrate the utility of this model system for evaluating candidate human epilepsy genes.", null, null, null, null, "Kcnd2 Hom 4", null, "strain:Casper|age:5dpf|collection date:2022 12 09|geo loc name:USA: Boston  MA|sex:not applicable|tissue:Head|genotype:Kcnd2 Hom4|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio Rerio: pools of isolated larval head", "Kcnd2 Hom 4", "Kcnd2 Hom 4", "Three replicate larvae from Het in crosses  aged 5 dpf  were sacrificed on ice and stored in RNAlater Stabilization Solution according to product specifications. Larval heads were isolated from bodies  which were used for Sanger sequencing for genotyping. Heads were pooled in groups of 20 25 according to genotype and RNA was isolated using a Qiagen RNeasy plus kit according to specifications. QC was performed via Agilent 2100 bioanalyzer  and only samples with an RNA integrity number value of >5.8 were used for library preparation.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP507312", null, null, "Kcnd2_Hom_4_1.fq.gz Kcnd2_Hom_4_2.fq.gz", "fastq fastq", 7596974100.0, 25323247.0, "Kcnd2 Hom 4 1.fq.gz", "0:150 1:150", "A:2007694949;C:1807188755;G:1800403701;T:1981369650;N:317045", 150, 150, null, null, 2007694949, 1807188755, 1800403701, 1981369650, 317045, "SRX24534618", "SRS21280301", "SRA1864976", "Boston Childrens Hospital|Neurobiology", "Boston Childrens Hospital", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-05-13", "Larval", "Larval", "Head", "Nervous System"], [32086, "SRR29007575", "SRX24534617", "SRS21280300", "SRP507312", "PRJNA1111077", "Zebrafish models of candidate human epilepsy associated genes provide evidence of hyperexcitability", "PRJNA1111077", "Other", "Hundreds of novel candidate human epilepsy associated genes have been identified thanks to advancements in next generation sequencing and large genome wide association studies  but establishing genetic etiology requires functional validation. We generated a list of >2200 candidate epilepsy associated genes  of which 48 were developed into stable loss of function zebrafish models. Of those 48  evidence of seizure like behavior was present in 5 arfgef1  kcnd2  kcnv1  ubr5  wnt8b. Further characterization provided evidence for epileptiform activity via electrophysiology in kcnd2 and wnt8b mutants. Additionally  arfgef1 and wnt8b mutants showed a decrease in the number of inhibitory interneurons in the optic tectum of larval animals. Further  RNA Seq revealed convergent transcriptional abnormalities between mutant lines  consistent with their developmental defects and hyperexcitable phenotypes. These zebrafish models provide strongest experimental evidence supporting the role of ARFGEF1  KCND2  and WNT8B in human epilepsy and further demonstrate the utility of this model system for evaluating candidate human epilepsy genes.", null, null, null, null, "Kcnd2 Hom 2", null, "strain:Casper|age:5dpf|collection date:2022 12 09|geo loc name:USA: Boston  MA|sex:not applicable|tissue:Head|genotype:Kcnd2 Hom2|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio Rerio: pools of isolated larval head", "Kcnd2 Hom 2", "Kcnd2 Hom 2", "Three replicate larvae from Het in crosses  aged 5 dpf  were sacrificed on ice and stored in RNAlater Stabilization Solution according to product specifications. Larval heads were isolated from bodies  which were used for Sanger sequencing for genotyping. Heads were pooled in groups of 20 25 according to genotype and RNA was isolated using a Qiagen RNeasy plus kit according to specifications. QC was performed via Agilent 2100 bioanalyzer  and only samples with an RNA integrity number value of >5.8 were used for library preparation.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP507312", null, null, "Kcnd2_Hom_2_1.fq.gz Kcnd2_Hom_2_2.fq.gz", "fastq fastq", 7289261100.0, 24297537.0, "Kcnd2 Hom 2 1.fq.gz", "0:150 1:150", "A:2078143741;C:1581832453;G:1587225393;T:2041970189;N:89324", 150, 150, null, null, 2078143741, 1581832453, 1587225393, 2041970189, 89324, "SRX24534617", "SRS21280300", "SRA1864976", "Boston Childrens Hospital|Neurobiology", "Boston Childrens Hospital", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-05-13", "Larval", "Larval", "Head", "Nervous System"], [32087, "SRR29007576", "SRX24534616", "SRS21280299", "SRP507312", "PRJNA1111077", "Zebrafish models of candidate human epilepsy associated genes provide evidence of hyperexcitability", "PRJNA1111077", "Other", "Hundreds of novel candidate human epilepsy associated genes have been identified thanks to advancements in next generation sequencing and large genome wide association studies  but establishing genetic etiology requires functional validation. We generated a list of >2200 candidate epilepsy associated genes  of which 48 were developed into stable loss of function zebrafish models. Of those 48  evidence of seizure like behavior was present in 5 arfgef1  kcnd2  kcnv1  ubr5  wnt8b. Further characterization provided evidence for epileptiform activity via electrophysiology in kcnd2 and wnt8b mutants. Additionally  arfgef1 and wnt8b mutants showed a decrease in the number of inhibitory interneurons in the optic tectum of larval animals. Further  RNA Seq revealed convergent transcriptional abnormalities between mutant lines  consistent with their developmental defects and hyperexcitable phenotypes. These zebrafish models provide strongest experimental evidence supporting the role of ARFGEF1  KCND2  and WNT8B in human epilepsy and further demonstrate the utility of this model system for evaluating candidate human epilepsy genes.", null, null, null, null, "Kcnd2 Hom 1", null, "strain:Casper|age:5dpf|collection date:2022 12 09|geo loc name:USA: Boston  MA|sex:not applicable|tissue:Head|genotype:Kcnd2 Hom1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio Rerio: pools of isolated larval head", "Kcnd2 Hom 1", "Kcnd2 Hom 1", "Three replicate larvae from Het in crosses  aged 5 dpf  were sacrificed on ice and stored in RNAlater Stabilization Solution according to product specifications. Larval heads were isolated from bodies  which were used for Sanger sequencing for genotyping. Heads were pooled in groups of 20 25 according to genotype and RNA was isolated using a Qiagen RNeasy plus kit according to specifications. QC was performed via Agilent 2100 bioanalyzer  and only samples with an RNA integrity number value of >5.8 were used for library preparation.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP507312", null, null, "Kcnd2_Hom_1_1.fq.gz Kcnd2_Hom_1_2.fq.gz", "fastq fastq", 8331212400.0, 27770708.0, "Kcnd2 Hom 1 1.fq.gz", "0:150 1:150", "A:2185320924;C:1987553985;G:1999101969;T:2159195984;N:39538", 150, 150, null, null, 2185320924, 1987553985, 1999101969, 2159195984, 39538, "SRX24534616", "SRS21280299", "SRA1864976", "Boston Childrens Hospital|Neurobiology", "Boston Childrens Hospital", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-05-13", "Larval", "Larval", "Head", "Nervous System"], [32088, "SRR29007577", "SRX24534615", "SRS21280298", "SRP507312", "PRJNA1111077", "Zebrafish models of candidate human epilepsy associated genes provide evidence of hyperexcitability", "PRJNA1111077", "Other", "Hundreds of novel candidate human epilepsy associated genes have been identified thanks to advancements in next generation sequencing and large genome wide association studies  but establishing genetic etiology requires functional validation. We generated a list of >2200 candidate epilepsy associated genes  of which 48 were developed into stable loss of function zebrafish models. Of those 48  evidence of seizure like behavior was present in 5 arfgef1  kcnd2  kcnv1  ubr5  wnt8b. Further characterization provided evidence for epileptiform activity via electrophysiology in kcnd2 and wnt8b mutants. Additionally  arfgef1 and wnt8b mutants showed a decrease in the number of inhibitory interneurons in the optic tectum of larval animals. Further  RNA Seq revealed convergent transcriptional abnormalities between mutant lines  consistent with their developmental defects and hyperexcitable phenotypes. These zebrafish models provide strongest experimental evidence supporting the role of ARFGEF1  KCND2  and WNT8B in human epilepsy and further demonstrate the utility of this model system for evaluating candidate human epilepsy genes.", null, null, null, null, "Kcnd2 Het 4", null, "strain:Casper|age:5dpf|collection date:2022 12 09|geo loc name:USA: Boston  MA|sex:not applicable|tissue:Head|genotype:Kcnd2 Het4|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio Rerio: pools of isolated larval head", "Kcnd2 Het 4", "Kcnd2 Het 4", "Three replicate larvae from Het in crosses  aged 5 dpf  were sacrificed on ice and stored in RNAlater Stabilization Solution according to product specifications. Larval heads were isolated from bodies  which were used for Sanger sequencing for genotyping. Heads were pooled in groups of 20 25 according to genotype and RNA was isolated using a Qiagen RNeasy plus kit according to specifications. QC was performed via Agilent 2100 bioanalyzer  and only samples with an RNA integrity number value of >5.8 were used for library preparation.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP507312", null, null, "Kcnd2_Het_4_1.fq.gz Kcnd2_Het_4_2.fq.gz", "fastq fastq", 10195958700.0, 33986529.0, "Kcnd2 Het 4 1.fq.gz", "0:150 1:150", "A:2675050868;C:2442920285;G:2439165482;T:2638402351;N:419714", 150, 150, null, null, 2675050868, 2442920285, 2439165482, 2638402351, 419714, "SRX24534615", "SRS21280298", "SRA1864976", "Boston Childrens Hospital|Neurobiology", "Boston Childrens Hospital", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-05-13", "Larval", "Larval", "Head", "Nervous System"], [32089, "SRR29007578", "SRX24534614", "SRS21280297", "SRP507312", "PRJNA1111077", "Zebrafish models of candidate human epilepsy associated genes provide evidence of hyperexcitability", "PRJNA1111077", "Other", "Hundreds of novel candidate human epilepsy associated genes have been identified thanks to advancements in next generation sequencing and large genome wide association studies  but establishing genetic etiology requires functional validation. We generated a list of >2200 candidate epilepsy associated genes  of which 48 were developed into stable loss of function zebrafish models. Of those 48  evidence of seizure like behavior was present in 5 arfgef1  kcnd2  kcnv1  ubr5  wnt8b. Further characterization provided evidence for epileptiform activity via electrophysiology in kcnd2 and wnt8b mutants. Additionally  arfgef1 and wnt8b mutants showed a decrease in the number of inhibitory interneurons in the optic tectum of larval animals. Further  RNA Seq revealed convergent transcriptional abnormalities between mutant lines  consistent with their developmental defects and hyperexcitable phenotypes. These zebrafish models provide strongest experimental evidence supporting the role of ARFGEF1  KCND2  and WNT8B in human epilepsy and further demonstrate the utility of this model system for evaluating candidate human epilepsy genes.", null, null, null, null, "Kcnd2 Het 2", null, "strain:Casper|age:5dpf|collection date:2022 12 09|geo loc name:USA: Boston  MA|sex:not applicable|tissue:Head|genotype:Kcnd2 Het2|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio Rerio: pools of isolated larval head", "Kcnd2 Het 2", "Kcnd2 Het 2", "Three replicate larvae from Het in crosses  aged 5 dpf  were sacrificed on ice and stored in RNAlater Stabilization Solution according to product specifications. Larval heads were isolated from bodies  which were used for Sanger sequencing for genotyping. Heads were pooled in groups of 20 25 according to genotype and RNA was isolated using a Qiagen RNeasy plus kit according to specifications. QC was performed via Agilent 2100 bioanalyzer  and only samples with an RNA integrity number value of >5.8 were used for library preparation.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP507312", null, null, "Kcnd2_Het_2_1.fq.gz Kcnd2_Het_2_2.fq.gz", "fastq fastq", 7549108800.0, 25163696.0, "Kcnd2 Het 2 1.fq.gz", "0:150 1:150", "A:1994641167;C:1795831385;G:1796069017;T:1962477823;N:89408", 150, 150, null, null, 1994641167, 1795831385, 1796069017, 1962477823, 89408, "SRX24534614", "SRS21280297", "SRA1864976", "Boston Childrens Hospital|Neurobiology", "Boston Childrens Hospital", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-05-13", "Larval", "Larval", "Head", "Nervous System"], [32090, "SRR29007579", "SRX24534613", "SRS21280296", "SRP507312", "PRJNA1111077", "Zebrafish models of candidate human epilepsy associated genes provide evidence of hyperexcitability", "PRJNA1111077", "Other", "Hundreds of novel candidate human epilepsy associated genes have been identified thanks to advancements in next generation sequencing and large genome wide association studies  but establishing genetic etiology requires functional validation. We generated a list of >2200 candidate epilepsy associated genes  of which 48 were developed into stable loss of function zebrafish models. Of those 48  evidence of seizure like behavior was present in 5 arfgef1  kcnd2  kcnv1  ubr5  wnt8b. Further characterization provided evidence for epileptiform activity via electrophysiology in kcnd2 and wnt8b mutants. Additionally  arfgef1 and wnt8b mutants showed a decrease in the number of inhibitory interneurons in the optic tectum of larval animals. Further  RNA Seq revealed convergent transcriptional abnormalities between mutant lines  consistent with their developmental defects and hyperexcitable phenotypes. These zebrafish models provide strongest experimental evidence supporting the role of ARFGEF1  KCND2  and WNT8B in human epilepsy and further demonstrate the utility of this model system for evaluating candidate human epilepsy genes.", null, null, null, null, "Kcnd2 Het 1", null, "strain:Casper|age:5dpf|collection date:2022 12 09|geo loc name:USA: Boston  MA|sex:not applicable|tissue:Head|genotype:Kcnd2 Het1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio Rerio: pools of isolated larval head", "Kcnd2 Het 1", "Kcnd2 Het 1", "Three replicate larvae from Het in crosses  aged 5 dpf  were sacrificed on ice and stored in RNAlater Stabilization Solution according to product specifications. Larval heads were isolated from bodies  which were used for Sanger sequencing for genotyping. Heads were pooled in groups of 20 25 according to genotype and RNA was isolated using a Qiagen RNeasy plus kit according to specifications. QC was performed via Agilent 2100 bioanalyzer  and only samples with an RNA integrity number value of >5.8 were used for library preparation.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP507312", null, null, "Kcnd2_Het_1_1.fq.gz Kcnd2_Het_1_2.fq.gz", "fastq fastq", 7081857600.0, 23606192.0, "Kcnd2 Het 1 1.fq.gz", "0:150 1:150", "A:1860484446;C:1694088253;G:1695161253;T:1832038793;N:84855", 150, 150, null, null, 1860484446, 1694088253, 1695161253, 1832038793, 84855, "SRX24534613", "SRS21280296", "SRA1864976", "Boston Childrens Hospital|Neurobiology", "Boston Childrens Hospital", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-05-13", "Larval", "Larval", "Head", "Nervous System"], [32091, "SRR29007580", "SRX24534612", "SRS21280295", "SRP507312", "PRJNA1111077", "Zebrafish models of candidate human epilepsy associated genes provide evidence of hyperexcitability", "PRJNA1111077", "Other", "Hundreds of novel candidate human epilepsy associated genes have been identified thanks to advancements in next generation sequencing and large genome wide association studies  but establishing genetic etiology requires functional validation. We generated a list of >2200 candidate epilepsy associated genes  of which 48 were developed into stable loss of function zebrafish models. Of those 48  evidence of seizure like behavior was present in 5 arfgef1  kcnd2  kcnv1  ubr5  wnt8b. Further characterization provided evidence for epileptiform activity via electrophysiology in kcnd2 and wnt8b mutants. Additionally  arfgef1 and wnt8b mutants showed a decrease in the number of inhibitory interneurons in the optic tectum of larval animals. Further  RNA Seq revealed convergent transcriptional abnormalities between mutant lines  consistent with their developmental defects and hyperexcitable phenotypes. These zebrafish models provide strongest experimental evidence supporting the role of ARFGEF1  KCND2  and WNT8B in human epilepsy and further demonstrate the utility of this model system for evaluating candidate human epilepsy genes.", null, null, null, null, "Kcnd2 Wt 4", null, "strain:Casper|age:5dpf|collection date:2022 12 09|geo loc name:USA: Boston  MA|sex:not applicable|tissue:Head|genotype:Kcnd2 Wt4|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio Rerio: pools of isolated larval head", "Kcnd2 Wt 4", "Kcnd2 Wt 4", "Three replicate larvae from Het in crosses  aged 5 dpf  were sacrificed on ice and stored in RNAlater Stabilization Solution according to product specifications. Larval heads were isolated from bodies  which were used for Sanger sequencing for genotyping. Heads were pooled in groups of 20 25 according to genotype and RNA was isolated using a Qiagen RNeasy plus kit according to specifications. QC was performed via Agilent 2100 bioanalyzer  and only samples with an RNA integrity number value of >5.8 were used for library preparation.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP507312", null, null, "Kcnd2_Wt_4_1.fq.gz Kcnd2_Wt_4_2.fq.gz", "fastq fastq", 8142446700.0, 27141489.0, "Kcnd2 Wt 4 1.fq.gz", "0:150 1:150", "A:2136890254;C:1951822302;G:1947544318;T:2105854363;N:335463", 150, 150, null, null, 2136890254, 1951822302, 1947544318, 2105854363, 335463, "SRX24534612", "SRS21280295", "SRA1864976", "Boston Childrens Hospital|Neurobiology", "Boston Childrens Hospital", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-05-13", "Larval", "Larval", "Head", "Nervous System"], [32092, "SRR29007581", "SRX24534611", "SRS21280294", "SRP507312", "PRJNA1111077", "Zebrafish models of candidate human epilepsy associated genes provide evidence of hyperexcitability", "PRJNA1111077", "Other", "Hundreds of novel candidate human epilepsy associated genes have been identified thanks to advancements in next generation sequencing and large genome wide association studies  but establishing genetic etiology requires functional validation. We generated a list of >2200 candidate epilepsy associated genes  of which 48 were developed into stable loss of function zebrafish models. Of those 48  evidence of seizure like behavior was present in 5 arfgef1  kcnd2  kcnv1  ubr5  wnt8b. Further characterization provided evidence for epileptiform activity via electrophysiology in kcnd2 and wnt8b mutants. Additionally  arfgef1 and wnt8b mutants showed a decrease in the number of inhibitory interneurons in the optic tectum of larval animals. Further  RNA Seq revealed convergent transcriptional abnormalities between mutant lines  consistent with their developmental defects and hyperexcitable phenotypes. These zebrafish models provide strongest experimental evidence supporting the role of ARFGEF1  KCND2  and WNT8B in human epilepsy and further demonstrate the utility of this model system for evaluating candidate human epilepsy genes.", null, null, null, null, "Kcnd2 Wt 2", null, "strain:Casper|age:5dpf|collection date:2022 12 09|geo loc name:USA: Boston  MA|sex:not applicable|tissue:Head|genotype:Kcnd2 Wt2|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio Rerio: pools of isolated larval head", "Kcnd2 Wt 2", "Kcnd2 Wt 2", "Three replicate larvae from Het in crosses  aged 5 dpf  were sacrificed on ice and stored in RNAlater Stabilization Solution according to product specifications. Larval heads were isolated from bodies  which were used for Sanger sequencing for genotyping. Heads were pooled in groups of 20 25 according to genotype and RNA was isolated using a Qiagen RNeasy plus kit according to specifications. QC was performed via Agilent 2100 bioanalyzer  and only samples with an RNA integrity number value of >5.8 were used for library preparation.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP507312", null, null, "Kcnd2_Wt_2_1.fq.gz Kcnd2_Wt_2_2.fq.gz", "fastq fastq", 6688680600.0, 22295602.0, "Kcnd2 Wt 2 1.fq.gz", "0:150 1:150", "A:1902466623;C:1451301657;G:1460696794;T:1874184765;N:30761", 150, 150, null, null, 1902466623, 1451301657, 1460696794, 1874184765, 30761, "SRX24534611", "SRS21280294", "SRA1864976", "Boston Childrens Hospital|Neurobiology", "Boston Childrens Hospital", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-05-13", "Larval", "Larval", "Head", "Nervous System"], [32093, "SRR29007582", "SRX24534610", "SRS21280293", "SRP507312", "PRJNA1111077", "Zebrafish models of candidate human epilepsy associated genes provide evidence of hyperexcitability", "PRJNA1111077", "Other", "Hundreds of novel candidate human epilepsy associated genes have been identified thanks to advancements in next generation sequencing and large genome wide association studies  but establishing genetic etiology requires functional validation. We generated a list of >2200 candidate epilepsy associated genes  of which 48 were developed into stable loss of function zebrafish models. Of those 48  evidence of seizure like behavior was present in 5 arfgef1  kcnd2  kcnv1  ubr5  wnt8b. Further characterization provided evidence for epileptiform activity via electrophysiology in kcnd2 and wnt8b mutants. Additionally  arfgef1 and wnt8b mutants showed a decrease in the number of inhibitory interneurons in the optic tectum of larval animals. Further  RNA Seq revealed convergent transcriptional abnormalities between mutant lines  consistent with their developmental defects and hyperexcitable phenotypes. These zebrafish models provide strongest experimental evidence supporting the role of ARFGEF1  KCND2  and WNT8B in human epilepsy and further demonstrate the utility of this model system for evaluating candidate human epilepsy genes.", null, null, null, null, "Arfgef1 Wt 2", null, "strain:Casper|age:5dpf|collection date:2022 12 09|geo loc name:USA: Boston  MA|sex:not applicable|tissue:Head|genotype:Arfgef1 Wt2|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio Rerio: pools of isolated larval head", "Arfgef1 Wt 2", "Arfgef1 Wt 2", "Three replicate larvae from Het in crosses  aged 5 dpf  were sacrificed on ice and stored in RNAlater Stabilization Solution according to product specifications. Larval heads were isolated from bodies  which were used for Sanger sequencing for genotyping. Heads were pooled in groups of 20 25 according to genotype and RNA was isolated using a Qiagen RNeasy plus kit according to specifications. QC was performed via Agilent 2100 bioanalyzer  and only samples with an RNA integrity number value of >5.8 were used for library preparation.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP507312", null, null, "Arfgef1_Wt_2_1.fq.gz Arfgef1_Wt_2_2.fq.gz", "fastq fastq", 7399981500.0, 24666605.0, "Arfgef1 Wt 2 1.fq.gz", "0:150 1:150", "A:1904125951;C:1807905407;G:1812898141;T:1874904149;N:147852", 150, 150, null, null, 1904125951, 1807905407, 1812898141, 1874904149, 147852, "SRX24534610", "SRS21280293", "SRA1864976", "Boston Childrens Hospital|Neurobiology", "Boston Childrens Hospital", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-05-13", "Larval", "Larval", "Head", "Nervous System"], [32094, "SRR29007583", "SRX24534609", "SRS21280292", "SRP507312", "PRJNA1111077", "Zebrafish models of candidate human epilepsy associated genes provide evidence of hyperexcitability", "PRJNA1111077", "Other", "Hundreds of novel candidate human epilepsy associated genes have been identified thanks to advancements in next generation sequencing and large genome wide association studies  but establishing genetic etiology requires functional validation. We generated a list of >2200 candidate epilepsy associated genes  of which 48 were developed into stable loss of function zebrafish models. Of those 48  evidence of seizure like behavior was present in 5 arfgef1  kcnd2  kcnv1  ubr5  wnt8b. Further characterization provided evidence for epileptiform activity via electrophysiology in kcnd2 and wnt8b mutants. Additionally  arfgef1 and wnt8b mutants showed a decrease in the number of inhibitory interneurons in the optic tectum of larval animals. Further  RNA Seq revealed convergent transcriptional abnormalities between mutant lines  consistent with their developmental defects and hyperexcitable phenotypes. These zebrafish models provide strongest experimental evidence supporting the role of ARFGEF1  KCND2  and WNT8B in human epilepsy and further demonstrate the utility of this model system for evaluating candidate human epilepsy genes.", null, null, null, null, "Arfgef1 Wt 1", null, "strain:Casper|age:5dpf|collection date:2022 12 09|geo loc name:USA: Boston  MA|sex:not applicable|tissue:Head|genotype:Arfgef1 Wt1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio Rerio: pools of isolated larval head", "Arfgef1 Wt 1", "Arfgef1 Wt 1", "Three replicate larvae from Het in crosses  aged 5 dpf  were sacrificed on ice and stored in RNAlater Stabilization Solution according to product specifications. Larval heads were isolated from bodies  which were used for Sanger sequencing for genotyping. Heads were pooled in groups of 20 25 according to genotype and RNA was isolated using a Qiagen RNeasy plus kit according to specifications. QC was performed via Agilent 2100 bioanalyzer  and only samples with an RNA integrity number value of >5.8 were used for library preparation.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP507312", null, null, "Arfgef1_Wt_1_1.fq.gz Arfgef1_Wt_1_2.fq.gz", "fastq fastq", 7542057300.0, 25140191.0, "Arfgef1 Wt 1 1.fq.gz", "0:150 1:150", "A:1927852618;C:1855757148;G:1860100302;T:1898222485;N:124747", 150, 150, null, null, 1927852618, 1855757148, 1860100302, 1898222485, 124747, "SRX24534609", "SRS21280292", "SRA1864976", "Boston Childrens Hospital|Neurobiology", "Boston Childrens Hospital", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-05-13", "Larval", "Larval", "Head", "Nervous System"], [32098, "SRR29008446", "SRX24535482", "SRS21281082", "SRP507332", "PRJNA1111082", "Integration of ATAC and RNA Sequencing identifies chromatin and transcriptomic signatures in classical and non classical zebrafish osteoblasts and indicates mechanisms of entpd5a regulation [RNA]", "GSE267333", "Transcriptome Analysis", "Two types of osteoblasts are required to assemble the zebrafish embryonic skeleton: classical osteoblasts homologous to the mammalian situation  and notochord sheath cells  which serve as non classical osteoblasts. The gene entpd5a is critically required for ossification via both types of osteoblasts. Despite the usefulness of zebrafish models in vertebrate research  the genetic regulation of bone formation  as well as mechanisms of transcriptional control of entpd5a  remain largely unknown. Here  using a newly generated transgenic line  we isolate classical and non classical osteoblasts from zebrafish embryos and performed both ATAC Seq and RNA Seq. We analysed results independently and integratively to understand those chromatin dynamics and accompanying transcriptomic changes that occur in different skeletal cells. We show that although Dlx family factors are playing important roles in classical osteoblast regulation  Hox family factors are involved in governing spinal ossification via non classical osteoblasts. We further present a resource driven analysis of the entpd5a promoter  experimentally validating the ATAC Seq dataset and proposing mechanisms of regulating the complex entpd5a expression pattern during zebrafish osteogenesis. Our results thus provide a necessary comprehensive resource for the field of bone development and indicate spatio temporally regulated promoter/enhancer interactions taking place in the entpd5a locus. Overall design: We used the newly generated entpd5a:Gal4FF;UAS:GFP;R2col2a1a:mCherry transgenic line to isolate via FACS head classical and trunk non classical osteoblasts from zebrafish embryos at 15dpf. We also extracted adjacent head cartilage and intersegmental spinal chondrocytes as controls. With these four isolated cell populations we performed both ATAC Seq and RNA Seq and analysed data at first individually  to establish open chromatin regions and gene expression profiles  respectively. We then integrated the data to enable us to produce a list of candidate transcription factors likely to function in vivo in each of the cell types during zebrafish skeletogenesis.", null, null, null, "Chondrocyte head biolRep3", "GSM8264152", null, "source name:head|tissue:head|cell type:head chondrocyte|genotype:WT; entpd5a:Gal4FF;UAS:GFP;R2col2a1a:mCherry|Stage:15 dpf loc name:missing|collection date:missing", "Chondrocyte head biolRep3", "QC of sequences  adapter trimming and duplicate removal using fastp package with   dedup option Sequence alignment to genome using the STAR aligner samtools to sort and index aligned reads reads counted using the featureCounts command from the Subread package Assembly: danRer11 Supplementary files format and content: tab deliminated text files including raw count for each sample 7th column", "head", null, "RNA was isolated using the RNeasy Plus Micro Kit QIAGEN  74034. Samples were sent to Eurofins Genomics for library preparation and paired end sequencing with the INVIEW Transcriptome Ultra Low service 2x 150. Per sample  15M reads were sequenced.", null, "tissue:head|cell type:head chondrocyte|genotype:WT; entpd5a:Gal4FF;UAS:GFP;R2col2a1a:mCherry|Stage:15 dpf", "GSM8264152", "GSM8264152: Chondrocyte head biolRep3; Danio rerio; RNA Seq", "GSM8264152 r1", "GSM8264152", "1", "RNA was isolated using the RNeasy Plus Micro Kit QIAGEN  74034. Samples were sent to Eurofins Genomics for library preparation and paired end sequencing with the INVIEW Transcriptome Ultra Low service 2x 150. Per sample  15M reads were sequenced.", null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP507332", null, "loader:fastq load.py", "cartilRep3_R1.fastq.gz cartilRep3_R2.fastq.gz", "fastq fastq", 6819648393.0, 25781563.0, "GSM8264152 r1", "0:132.28 1:132.24", "A:1843991471;C:1548648708;G:1568393744;T:1858508350;N:106120", 132, 132, null, null, 1843991471, 1548648708, 1568393744, 1858508350, 106120, "SRX24535482", "SRS21281082", "SRA1865629", "Institute for Cardiovascular Organogenesis and Regeneration", "Institute for Cardiovascular Organogenesis and Regeneration", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "Germany", "2024-05-13", "Larval", "Larval", "Head", "Nervous System"], [32099, "SRR29008447", "SRX24535481", "SRS21281081", "SRP507332", "PRJNA1111082", "Integration of ATAC and RNA Sequencing identifies chromatin and transcriptomic signatures in classical and non classical zebrafish osteoblasts and indicates mechanisms of entpd5a regulation [RNA]", "GSE267333", "Transcriptome Analysis", "Two types of osteoblasts are required to assemble the zebrafish embryonic skeleton: classical osteoblasts homologous to the mammalian situation  and notochord sheath cells  which serve as non classical osteoblasts. The gene entpd5a is critically required for ossification via both types of osteoblasts. Despite the usefulness of zebrafish models in vertebrate research  the genetic regulation of bone formation  as well as mechanisms of transcriptional control of entpd5a  remain largely unknown. Here  using a newly generated transgenic line  we isolate classical and non classical osteoblasts from zebrafish embryos and performed both ATAC Seq and RNA Seq. We analysed results independently and integratively to understand those chromatin dynamics and accompanying transcriptomic changes that occur in different skeletal cells. We show that although Dlx family factors are playing important roles in classical osteoblast regulation  Hox family factors are involved in governing spinal ossification via non classical osteoblasts. We further present a resource driven analysis of the entpd5a promoter  experimentally validating the ATAC Seq dataset and proposing mechanisms of regulating the complex entpd5a expression pattern during zebrafish osteogenesis. Our results thus provide a necessary comprehensive resource for the field of bone development and indicate spatio temporally regulated promoter/enhancer interactions taking place in the entpd5a locus. Overall design: We used the newly generated entpd5a:Gal4FF;UAS:GFP;R2col2a1a:mCherry transgenic line to isolate via FACS head classical and trunk non classical osteoblasts from zebrafish embryos at 15dpf. We also extracted adjacent head cartilage and intersegmental spinal chondrocytes as controls. With these four isolated cell populations we performed both ATAC Seq and RNA Seq and analysed data at first individually  to establish open chromatin regions and gene expression profiles  respectively. We then integrated the data to enable us to produce a list of candidate transcription factors likely to function in vivo in each of the cell types during zebrafish skeletogenesis.", null, null, null, "Chondrocyte head biolRep2", "GSM8264151", null, "source name:head|tissue:head|cell type:head chondrocyte|genotype:WT; entpd5a:Gal4FF;UAS:GFP;R2col2a1a:mCherry|Stage:15 dpf loc name:missing|collection date:missing", "Chondrocyte head biolRep2", "QC of sequences  adapter trimming and duplicate removal using fastp package with   dedup option Sequence alignment to genome using the STAR aligner samtools to sort and index aligned reads reads counted using the featureCounts command from the Subread package Assembly: danRer11 Supplementary files format and content: tab deliminated text files including raw count for each sample 7th column", "head", null, "RNA was isolated using the RNeasy Plus Micro Kit QIAGEN  74034. Samples were sent to Eurofins Genomics for library preparation and paired end sequencing with the INVIEW Transcriptome Ultra Low service 2x 150. Per sample  15M reads were sequenced.", null, "tissue:head|cell type:head chondrocyte|genotype:WT; entpd5a:Gal4FF;UAS:GFP;R2col2a1a:mCherry|Stage:15 dpf", "GSM8264151", "GSM8264151: Chondrocyte head biolRep2; Danio rerio; RNA Seq", "GSM8264151 r1", "GSM8264151", "1", "RNA was isolated using the RNeasy Plus Micro Kit QIAGEN  74034. Samples were sent to Eurofins Genomics for library preparation and paired end sequencing with the INVIEW Transcriptome Ultra Low service 2x 150. Per sample  15M reads were sequenced.", null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP507332", null, "loader:fastq load.py", "cartilRep2_R1.fastq.gz cartilRep2_R2.fastq.gz", "fastq fastq", 6328377834.0, 23616303.0, "GSM8264151 r1", "0:134.02 1:133.95", "A:1697207927;C:1464700434;G:1476121489;T:1690068467;N:279517", 134, 133, null, null, 1697207927, 1464700434, 1476121489, 1690068467, 279517, "SRX24535481", "SRS21281081", "SRA1865629", "Institute for Cardiovascular Organogenesis and Regeneration", "Institute for Cardiovascular Organogenesis and Regeneration", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "Germany", "2024-05-13", "Larval", "Larval", "Head", "Nervous System"], [32100, "SRR29008448", "SRX24535480", "SRS21281080", "SRP507332", "PRJNA1111082", "Integration of ATAC and RNA Sequencing identifies chromatin and transcriptomic signatures in classical and non classical zebrafish osteoblasts and indicates mechanisms of entpd5a regulation [RNA]", "GSE267333", "Transcriptome Analysis", "Two types of osteoblasts are required to assemble the zebrafish embryonic skeleton: classical osteoblasts homologous to the mammalian situation  and notochord sheath cells  which serve as non classical osteoblasts. The gene entpd5a is critically required for ossification via both types of osteoblasts. Despite the usefulness of zebrafish models in vertebrate research  the genetic regulation of bone formation  as well as mechanisms of transcriptional control of entpd5a  remain largely unknown. Here  using a newly generated transgenic line  we isolate classical and non classical osteoblasts from zebrafish embryos and performed both ATAC Seq and RNA Seq. We analysed results independently and integratively to understand those chromatin dynamics and accompanying transcriptomic changes that occur in different skeletal cells. We show that although Dlx family factors are playing important roles in classical osteoblast regulation  Hox family factors are involved in governing spinal ossification via non classical osteoblasts. We further present a resource driven analysis of the entpd5a promoter  experimentally validating the ATAC Seq dataset and proposing mechanisms of regulating the complex entpd5a expression pattern during zebrafish osteogenesis. Our results thus provide a necessary comprehensive resource for the field of bone development and indicate spatio temporally regulated promoter/enhancer interactions taking place in the entpd5a locus. Overall design: We used the newly generated entpd5a:Gal4FF;UAS:GFP;R2col2a1a:mCherry transgenic line to isolate via FACS head classical and trunk non classical osteoblasts from zebrafish embryos at 15dpf. We also extracted adjacent head cartilage and intersegmental spinal chondrocytes as controls. With these four isolated cell populations we performed both ATAC Seq and RNA Seq and analysed data at first individually  to establish open chromatin regions and gene expression profiles  respectively. We then integrated the data to enable us to produce a list of candidate transcription factors likely to function in vivo in each of the cell types during zebrafish skeletogenesis.", null, null, null, "Chondrocyte head biolRep1", "GSM8264150", null, "source name:head|tissue:head|cell type:head chondrocyte|genotype:WT; entpd5a:Gal4FF;UAS:GFP;R2col2a1a:mCherry|Stage:15 dpf loc name:missing|collection date:missing", "Chondrocyte head biolRep1", "QC of sequences  adapter trimming and duplicate removal using fastp package with   dedup option Sequence alignment to genome using the STAR aligner samtools to sort and index aligned reads reads counted using the featureCounts command from the Subread package Assembly: danRer11 Supplementary files format and content: tab deliminated text files including raw count for each sample 7th column", "head", null, "RNA was isolated using the RNeasy Plus Micro Kit QIAGEN  74034. Samples were sent to Eurofins Genomics for library preparation and paired end sequencing with the INVIEW Transcriptome Ultra Low service 2x 150. Per sample  15M reads were sequenced.", null, "tissue:head|cell type:head chondrocyte|genotype:WT; entpd5a:Gal4FF;UAS:GFP;R2col2a1a:mCherry|Stage:15 dpf", "GSM8264150", "GSM8264150: Chondrocyte head biolRep1; Danio rerio; RNA Seq", "GSM8264150 r1", "GSM8264150", "1", "RNA was isolated using the RNeasy Plus Micro Kit QIAGEN  74034. Samples were sent to Eurofins Genomics for library preparation and paired end sequencing with the INVIEW Transcriptome Ultra Low service 2x 150. Per sample  15M reads were sequenced.", null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP507332", null, "loader:fastq load.py", "cartilRep1_R1.fastq.gz cartilRep1_R2.fastq.gz", "fastq fastq", 3594349367.0, 13391209.0, "GSM8264150 r1", "0:134.24 1:134.17", "A:956150712;C:837180050;G:846748291;T:954113728;N:156586", 134, 134, null, null, 956150712, 837180050, 846748291, 954113728, 156586, "SRX24535480", "SRS21281080", "SRA1865629", "Institute for Cardiovascular Organogenesis and Regeneration", "Institute for Cardiovascular Organogenesis and Regeneration", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "Germany", "2024-05-13", "Larval", "Larval", "Head", "Nervous System"]], "truncated": false, "filtered_table_rows_count": 408, "expanded_columns": [], "expandable_columns": [], "columns": ["rowid", "run.accession", "experiment.accession", "sample.accession", "study.accession", "bioproject", "study.title", "study.alias", "study.type", "study.abstract", "study.attributes", "study.PMIDs", "sample.description", "sample.title", "sample.alias", "sample.centername", "sample.attributes", "GEOsample.title", "GEOsample.dataprocessing", "GEOsample.source", "GEOsample.treatmentprotocol", "GEOsample.extractprotocol", "GEOsample.growthprotocol", "GEOsample.characteristics", "GEOsample.accession", "experiment.title", "experiment.alias", "experiment.library_name", "experiment.design_description", "experiment.library_construction_protocol", "experiment.attributes", "experiment.library_strategy", "experiment.library_source", "experiment.library_selection", "experiment.library_layout", "experiment.platform", "experiment.instrument_model", "experiment.spot_descriptor", "experiment.study_ref", "run.title", "run.attributes", "run.filename", "run.semantic_name", "run.total_bases", "run.total_spots", "run.alias", "run.read_lengths", "run.base_counts", "run.r1_length", "run.r2_length", "run.r3_length", "run.r4_length", "run.Acount", "run.Ccount", "run.Gcount", "run.Tcount", "run.Ncount", "run.experiment", "run.pool_member", "submission.accession", "submission.srasource", "submission.bioprojectsource", "seqdetective.n_mates", "seqdetective.mapping_rate.mate1", "seqdetective.mapping_rate.mate2", "seqdetective.nofeature_rate.mate1", "seqdetective.nofeature_rate.mate2", "seqdetective.sparsity.mate1", "seqdetective.sparsity.mate2", "seqdetective.pos_strand_rate.mate1", "seqdetective.pos_strand_rate.mate2", "seqdetective.readlen.mate1", "seqdetective.readlen.mate2", "seqdetective.judgement.mate1", "seqdetective.judgement.mate2", "seqdetective.judgement.reason", "platform_family", "instrument_generation", "read_bias", "selection_class", "prep_kit", "sc_or_bulk", "tech_class", "technology", "tech_variant", "submission.bioprojectsource.country", "earliest_date", "devstage_curation", "devstage_curation_coarse", "tissue_curation", "tissue_curation_coarse"], "primary_keys": [], "units": {}, "query": {"sql": "select rowid, [run.accession], [experiment.accession], [sample.accession], [study.accession], bioproject, [study.title], [study.alias], [study.type], [study.abstract], [study.attributes], [study.PMIDs], [sample.description], [sample.title], [sample.alias], [sample.centername], [sample.attributes], [GEOsample.title], [GEOsample.dataprocessing], [GEOsample.source], [GEOsample.treatmentprotocol], [GEOsample.extractprotocol], [GEOsample.growthprotocol], [GEOsample.characteristics], [GEOsample.accession], [experiment.title], [experiment.alias], [experiment.library_name], [experiment.design_description], [experiment.library_construction_protocol], [experiment.attributes], [experiment.library_strategy], [experiment.library_source], [experiment.library_selection], [experiment.library_layout], [experiment.platform], [experiment.instrument_model], [experiment.spot_descriptor], [experiment.study_ref], [run.title], [run.attributes], [run.filename], [run.semantic_name], [run.total_bases], [run.total_spots], [run.alias], [run.read_lengths], [run.base_counts], [run.r1_length], [run.r2_length], [run.r3_length], [run.r4_length], [run.Acount], [run.Ccount], [run.Gcount], [run.Tcount], [run.Ncount], [run.experiment], [run.pool_member], [submission.accession], [submission.srasource], [submission.bioprojectsource], [seqdetective.n_mates], [seqdetective.mapping_rate.mate1], [seqdetective.mapping_rate.mate2], [seqdetective.nofeature_rate.mate1], [seqdetective.nofeature_rate.mate2], [seqdetective.sparsity.mate1], [seqdetective.sparsity.mate2], [seqdetective.pos_strand_rate.mate1], [seqdetective.pos_strand_rate.mate2], [seqdetective.readlen.mate1], [seqdetective.readlen.mate2], [seqdetective.judgement.mate1], [seqdetective.judgement.mate2], [seqdetective.judgement.reason], platform_family, instrument_generation, read_bias, selection_class, prep_kit, sc_or_bulk, tech_class, technology, tech_variant, [submission.bioprojectsource.country], earliest_date, devstage_curation, devstage_curation_coarse, tissue_curation, tissue_curation_coarse from run_metadata where \"technology\" = :p0 and \"tissue_curation\" = :p1 and \"tissue_curation_coarse\" = :p2 order by rowid limit 101", "params": {"p0": "unknown", "p1": "Head", "p2": "Nervous System"}}, "facet_results": {"experiment.library_strategy": {"name": "experiment.library_strategy", "type": "column", "hideable": false, "toggle_url": "/metadata/run_metadata.json?technology=unknown&tissue_curation=Head&tissue_curation_coarse=Nervous+System", "results": [{"value": "RNA-Seq", "label": "RNA-Seq", "count": 396, "toggle_url": "http://metadata.rnaquarium.org/metadata/run_metadata.json?technology=unknown&tissue_curation=Head&tissue_curation_coarse=Nervous+System&experiment.library_strategy=RNA-Seq", "selected": false}, {"value": "OTHER", "label": "OTHER", "count": 10, "toggle_url": "http://metadata.rnaquarium.org/metadata/run_metadata.json?technology=unknown&tissue_curation=Head&tissue_curation_coarse=Nervous+System&experiment.library_strategy=OTHER", "selected": false}, {"value": "FL-cDNA", "label": "FL-cDNA", "count": 2, "toggle_url": "http://metadata.rnaquarium.org/metadata/run_metadata.json?technology=unknown&tissue_curation=Head&tissue_curation_coarse=Nervous+System&experiment.library_strategy=FL-cDNA", "selected": false}], "truncated": false}, "experiment.library_source": {"name": "experiment.library_source", "type": "column", "hideable": false, "toggle_url": "/metadata/run_metadata.json?technology=unknown&tissue_curation=Head&tissue_curation_coarse=Nervous+System", "results": [{"value": "TRANSCRIPTOMIC", "label": "TRANSCRIPTOMIC", "count": 408, "toggle_url": "http://metadata.rnaquarium.org/metadata/run_metadata.json?technology=unknown&tissue_curation=Head&tissue_curation_coarse=Nervous+System&experiment.library_source=TRANSCRIPTOMIC", "selected": false}], "truncated": false}, "experiment.library_selection": {"name": "experiment.library_selection", "type": "column", "hideable": false, "toggle_url": "/metadata/run_metadata.json?technology=unknown&tissue_curation=Head&tissue_curation_coarse=Nervous+System", "results": [{"value": "cDNA", "label": "cDNA", "count": 307, "toggle_url": "http://metadata.rnaquarium.org/metadata/run_metadata.json?technology=unknown&tissue_curation=Head&tissue_curation_coarse=Nervous+System&experiment.library_selection=cDNA", "selected": false}, {"value": "PolyA", "label": "PolyA", "count": 65, "toggle_url": "http://metadata.rnaquarium.org/metadata/run_metadata.json?technology=unknown&tissue_curation=Head&tissue_curation_coarse=Nervous+System&experiment.library_selection=PolyA", "selected": false}, {"value": "Oligo-dT", "label": "Oligo-dT", "count": 17, "toggle_url": "http://metadata.rnaquarium.org/metadata/run_metadata.json?technology=unknown&tissue_curation=Head&tissue_curation_coarse=Nervous+System&experiment.library_selection=Oligo-dT", "selected": false}, {"value": "other", "label": "other", "count": 11, "toggle_url": "http://metadata.rnaquarium.org/metadata/run_metadata.json?technology=unknown&tissue_curation=Head&tissue_curation_coarse=Nervous+System&experiment.library_selection=other", "selected": false}, {"value": "RT-PCR", "label": "RT-PCR", "count": 5, "toggle_url": "http://metadata.rnaquarium.org/metadata/run_metadata.json?technology=unknown&tissue_curation=Head&tissue_curation_coarse=Nervous+System&experiment.library_selection=RT-PCR", "selected": false}, {"value": "unspecified", "label": "unspecified", "count": 2, "toggle_url": "http://metadata.rnaquarium.org/metadata/run_metadata.json?technology=unknown&tissue_curation=Head&tissue_curation_coarse=Nervous+System&experiment.library_selection=unspecified", "selected": false}, {"value": "RANDOM", "label": "RANDOM", "count": 1, "toggle_url": "http://metadata.rnaquarium.org/metadata/run_metadata.json?technology=unknown&tissue_curation=Head&tissue_curation_coarse=Nervous+System&experiment.library_selection=RANDOM", "selected": false}], "truncated": false}, "experiment.library_layout": {"name": "experiment.library_layout", "type": "column", "hideable": false, "toggle_url": "/metadata/run_metadata.json?technology=unknown&tissue_curation=Head&tissue_curation_coarse=Nervous+System", "results": [{"value": "SINGLE", "label": "SINGLE", "count": 287, "toggle_url": "http://metadata.rnaquarium.org/metadata/run_metadata.json?technology=unknown&tissue_curation=Head&tissue_curation_coarse=Nervous+System&experiment.library_layout=SINGLE", "selected": false}, {"value": "PAIRED", "label": "PAIRED", "count": 121, "toggle_url": "http://metadata.rnaquarium.org/metadata/run_metadata.json?technology=unknown&tissue_curation=Head&tissue_curation_coarse=Nervous+System&experiment.library_layout=PAIRED", "selected": false}], "truncated": false}, "experiment.platform": {"name": "experiment.platform", "type": "column", "hideable": false, "toggle_url": "/metadata/run_metadata.json?technology=unknown&tissue_curation=Head&tissue_curation_coarse=Nervous+System", "results": [{"value": "ILLUMINA", "label": "ILLUMINA", "count": 399, "toggle_url": "http://metadata.rnaquarium.org/metadata/run_metadata.json?technology=unknown&tissue_curation=Head&tissue_curation_coarse=Nervous+System&experiment.platform=ILLUMINA", "selected": false}, {"value": "BGISEQ", "label": "BGISEQ", "count": 9, "toggle_url": "http://metadata.rnaquarium.org/metadata/run_metadata.json?technology=unknown&tissue_curation=Head&tissue_curation_coarse=Nervous+System&experiment.platform=BGISEQ", "selected": false}], "truncated": false}, "devstage_curation_coarse": {"name": "devstage_curation_coarse", "type": "column", "hideable": false, "toggle_url": "/metadata/run_metadata.json?technology=unknown&tissue_curation=Head&tissue_curation_coarse=Nervous+System", "results": [{"value": "Larval", "label": "Larval", "count": 182, "toggle_url": "http://metadata.rnaquarium.org/metadata/run_metadata.json?technology=unknown&tissue_curation=Head&tissue_curation_coarse=Nervous+System&devstage_curation_coarse=Larval", "selected": false}, {"value": "Embryo", "label": "Embryo", "count": 154, "toggle_url": "http://metadata.rnaquarium.org/metadata/run_metadata.json?technology=unknown&tissue_curation=Head&tissue_curation_coarse=Nervous+System&devstage_curation_coarse=Embryo", "selected": false}, {"value": "Adult", "label": "Adult", "count": 38, "toggle_url": "http://metadata.rnaquarium.org/metadata/run_metadata.json?technology=unknown&tissue_curation=Head&tissue_curation_coarse=Nervous+System&devstage_curation_coarse=Adult", "selected": false}, {"value": "Multi-stage", "label": "Multi-stage", "count": 30, "toggle_url": "http://metadata.rnaquarium.org/metadata/run_metadata.json?technology=unknown&tissue_curation=Head&tissue_curation_coarse=Nervous+System&devstage_curation_coarse=Multi-stage", "selected": false}, {"value": "Undetermined", "label": "Undetermined", "count": 4, "toggle_url": "http://metadata.rnaquarium.org/metadata/run_metadata.json?technology=unknown&tissue_curation=Head&tissue_curation_coarse=Nervous+System&devstage_curation_coarse=Undetermined", "selected": false}], "truncated": false}, "devstage_curation": {"name": "devstage_curation", "type": "column", "hideable": false, "toggle_url": "/metadata/run_metadata.json?technology=unknown&tissue_curation=Head&tissue_curation_coarse=Nervous+System", "results": [{"value": "Larval", "label": "Larval", "count": 182, "toggle_url": "http://metadata.rnaquarium.org/metadata/run_metadata.json?technology=unknown&tissue_curation=Head&tissue_curation_coarse=Nervous+System&devstage_curation=Larval", "selected": false}, {"value": "Multi-stage", "label": "Multi-stage", "count": 86, "toggle_url": "http://metadata.rnaquarium.org/metadata/run_metadata.json?technology=unknown&tissue_curation=Head&tissue_curation_coarse=Nervous+System&devstage_curation=Multi-stage", "selected": false}, {"value": "Hatching", "label": "Hatching", "count": 74, "toggle_url": "http://metadata.rnaquarium.org/metadata/run_metadata.json?technology=unknown&tissue_curation=Head&tissue_curation_coarse=Nervous+System&devstage_curation=Hatching", "selected": false}, {"value": "Adult", "label": "Adult", "count": 24, "toggle_url": "http://metadata.rnaquarium.org/metadata/run_metadata.json?technology=unknown&tissue_curation=Head&tissue_curation_coarse=Nervous+System&devstage_curation=Adult", "selected": false}, {"value": "Undetermined", "label": "Undetermined", "count": 18, "toggle_url": "http://metadata.rnaquarium.org/metadata/run_metadata.json?technology=unknown&tissue_curation=Head&tissue_curation_coarse=Nervous+System&devstage_curation=Undetermined", "selected": false}, {"value": "Pharyngula", "label": "Pharyngula", "count": 15, "toggle_url": "http://metadata.rnaquarium.org/metadata/run_metadata.json?technology=unknown&tissue_curation=Head&tissue_curation_coarse=Nervous+System&devstage_curation=Pharyngula", "selected": false}, {"value": "Segmentation", "label": "Segmentation", "count": 9, "toggle_url": "http://metadata.rnaquarium.org/metadata/run_metadata.json?technology=unknown&tissue_curation=Head&tissue_curation_coarse=Nervous+System&devstage_curation=Segmentation", "selected": false}], "truncated": false}, "tissue_curation_coarse": {"name": "tissue_curation_coarse", "type": "column", "hideable": false, "toggle_url": "/metadata/run_metadata.json?technology=unknown&tissue_curation=Head&tissue_curation_coarse=Nervous+System", "results": [{"value": "Nervous System", "label": "Nervous System", "count": 408, "toggle_url": "http://metadata.rnaquarium.org/metadata/run_metadata.json?technology=unknown&tissue_curation=Head", "selected": true}], "truncated": false}, "tissue_curation": {"name": "tissue_curation", "type": "column", "hideable": false, "toggle_url": "/metadata/run_metadata.json?technology=unknown&tissue_curation=Head&tissue_curation_coarse=Nervous+System", "results": [{"value": "Head", "label": "Head", "count": 408, "toggle_url": "http://metadata.rnaquarium.org/metadata/run_metadata.json?technology=unknown&tissue_curation_coarse=Nervous+System", "selected": true}], "truncated": false}, "technology": {"name": "technology", "type": "column", "hideable": false, "toggle_url": "/metadata/run_metadata.json?technology=unknown&tissue_curation=Head&tissue_curation_coarse=Nervous+System", "results": [{"value": "unknown", "label": "unknown", "count": 408, "toggle_url": "http://metadata.rnaquarium.org/metadata/run_metadata.json?tissue_curation=Head&tissue_curation_coarse=Nervous+System", "selected": true}], "truncated": false}}, "suggested_facets": [], "next": "32100", "next_url": "http://metadata.rnaquarium.org/metadata/run_metadata.json?technology=unknown&tissue_curation=Head&tissue_curation_coarse=Nervous+System&_next=32100", "private": false, "allow_execute_sql": true, "query_ms": 125.32094099151436}