{"database": "metadata", "table": "run_metadata", "is_view": false, "human_description_en": "where experiment.library_strategy = \"RNA-Seq\", experiment.platform = \"BGISEQ\" and tissue_curation = \"Cancer or Tumor\"", "rows": [[66210, "SRR16102702", "SRX12388690", "SRS10358692", "SRP339154", "PRJNA767083", "RNA seq analysis for IRF4 driven zebrafish tumors post JQ1 treatment", "GSE184946", "Transcriptome Analysis", "RNA seq analysis was performed using tumor cells driven by IRF4 to analyze gene expression changes post JQ1 treatment Overall design: The human IRF4 gene and mCherry fluorescent marker gene were transduced under the zebrafish lck promoter in zebrafish.  post the development of lymphoma phenotype  animals were treated with JQ1 or control DMSO for 1 or 3 days.  Tumor cells were harvested and sorted by flow cytometry based on mCherry fluorescence expression.  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Total RNAs were harvested from each sample and then applied for RNA seq analysis.", "cell type:Zebrafish tumor cells|condition:control|treatment:DMSO", "GSM5602247", "GSM5602247: Tumor cells  DMSO day3 rep2 RNA Seq; Danio rerio; RNA Seq", "GSM5602247", null, "1", "Total RNA was harvested using the miRNeasy Mini Kit Qiagen.  ERCC spike in was added based on the total cell number. 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Tumor cells were harvested and sorted by flow cytometry based on mCherry fluorescence expression.  Total RNAs were harvested from each sample and then applied for RNA seq analysis.", "parent bioproject:PRJNA701471", "pubmed:35504924", null, "Tumor cells  DMSO day3 rep1 RNA Seq", "GSM5602246", null, "tissue:Zebrafish tumor cells|cell type:Zebrafish tumor cells|condition:control|treatment:DMSO", "Tumor cells  DMSO day3 rep1 RNA Seq", "RNA seq datasets of DMSO treated control samples and JQ1 treated samples were aligned to the GRCz11 zebrafish genome Ensembl annotation with ERCC spike in information using STAR 2.5.2a with the parameter outFilterMultimapNmax set to 1. featureCount was used for the mapped reads in .bam files to generate count tables based on the Ensembl gene annotation and ERCC annotation. Bioconductor package DESeq2 v1.12.4 was used to analyze differential gene expression using 3 DMSO vs 3 JQ1 treated  samples. 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Tumor cells were harvested and sorted by flow cytometry based on mCherry fluorescence expression.  Total RNAs were harvested from each sample and then applied for RNA seq analysis.", "parent bioproject:PRJNA701471", "pubmed:35504924", null, "Tumor cells  JQ1 day1 rep3 RNA Seq", "GSM5602245", null, "tissue:Zebrafish tumor cells|cell type:Zebrafish tumor cells|condition:treatment|treatment:JQ1", "Tumor cells  JQ1 day1 rep3 RNA Seq", "RNA seq datasets of DMSO treated control samples and JQ1 treated samples were aligned to the GRCz11 zebrafish genome Ensembl annotation with ERCC spike in information using STAR 2.5.2a with the parameter outFilterMultimapNmax set to 1. featureCount was used for the mapped reads in .bam files to generate count tables based on the Ensembl gene annotation and ERCC annotation. Bioconductor package DESeq2 v1.12.4 was used to analyze differential gene expression using 3 DMSO vs 3 JQ1 treated  samples. 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Strand specific library construction and sequencing of paired end  100 bp long reads by the BGISeq500 were performed at the BGI Biotech Solutions Hong Kong Co Ltd Hong Kong.", "GEO Accession:GSM5602245", "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "BGISEQ", "BGISEQ-500", null, "SRP339154", null, null, "D1_J3_1.fq.gz D1_J3_2.fq.gz", "fastq fastq", 4550470000.0, 45504700.0, "GSM5602245 r1", "0:100 1:100", "A:1213052369;C:1047508174;G:1069309019;T:1220600438;N:0", 100, 100, null, null, 1213052369, 1047508174, 1069309019, 1220600438, 0, "SRX12388684", "SRS10358685", "SRA1302013", "GEO", "Takaomi Sanda Lab, Cancer Science Institute, National University of Singapore", 1, 0.94051, null, 0.24901, null, 0.74899, null, 0.50515, null, 100, null, "B", null, "usable mapping rate", "bgi", "bgi", "unknown", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "Singapore", "2021-09-28", "Undetermined", "Undetermined", "Cancer or Tumor", "Cancer or Tumor"], [66217, "SRR16102695", "SRX12388683", "SRS10358684", "SRP339154", "PRJNA767083", "RNA seq analysis for IRF4 driven zebrafish tumors post JQ1 treatment", "GSE184946", "Transcriptome Analysis", "RNA seq analysis was performed using tumor cells driven by IRF4 to analyze gene expression changes post JQ1 treatment Overall design: The human IRF4 gene and mCherry fluorescent marker gene were transduced under the zebrafish lck promoter in zebrafish.  post the development of lymphoma phenotype  animals were treated with JQ1 or control DMSO for 1 or 3 days.  Tumor cells were harvested and sorted by flow cytometry based on mCherry fluorescence expression.  Total RNAs were harvested from each sample and then applied for RNA seq analysis.", "parent bioproject:PRJNA701471", "pubmed:35504924", null, "Tumor cells  JQ1 day1 rep2 RNA Seq", "GSM5602244", null, "tissue:Zebrafish tumor cells|cell type:Zebrafish tumor cells|condition:treatment|treatment:JQ1", "Tumor cells  JQ1 day1 rep2 RNA Seq", "RNA seq datasets of DMSO treated control samples and JQ1 treated samples were aligned to the GRCz11 zebrafish genome Ensembl annotation with ERCC spike in information using STAR 2.5.2a with the parameter outFilterMultimapNmax set to 1. featureCount was used for the mapped reads in .bam files to generate count tables based on the Ensembl gene annotation and ERCC annotation. Bioconductor package DESeq2 v1.12.4 was used to analyze differential gene expression using 3 DMSO vs 3 JQ1 treated  samples. Genome build: danRer11 Supplementary files format and content: featureCount read count for all sample  DESeq2 files for differentially expressed genes", "Zebrafish tumor cells", "JQ1 was dissolved in DMSO.  Animals were treated with JQ1 at 4microM or the same amount of DMSO in individual tanks for 1 or 3 days.  Experiments were done using 3 independent animals.", "Total RNA was harvested using the miRNeasy Mini Kit Qiagen.  ERCC spike in was added based on the total cell number. Strand specific library construction and sequencing of paired end  100 bp long reads by the BGISeq500 were performed at the BGI Biotech Solutions Hong Kong Co Ltd Hong Kong.", "The human IRF4 gene and mCherry fluorescent marker gene were transduced under the zebrafish lck promoter in zebrafish.  post the development of lymphoma phenotype  animals were treated with JQ1.   Tumor cells were harvested and sorted by flow cytometry based on mCherry fluorescence expression.  Total RNAs were harvested from each sample and then applied for RNA seq analysis.", "cell type:Zebrafish tumor cells|condition:treatment|treatment:JQ1", "GSM5602244", "GSM5602244: Tumor cells  JQ1 day1 rep2 RNA Seq; Danio rerio; RNA Seq", "GSM5602244", null, "1", "Total RNA was harvested using the miRNeasy Mini Kit Qiagen.  ERCC spike in was added based on the total cell number. Strand specific library construction and sequencing of paired end  100 bp long reads by the BGISeq500 were performed at the BGI Biotech Solutions Hong Kong Co Ltd Hong Kong.", "GEO Accession:GSM5602244", "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "BGISEQ", "BGISEQ-500", null, "SRP339154", null, null, "D1_J2_1.fq.gz D1_J2_2.fq.gz", "fastq fastq", 4490144800.0, 44901448.0, "GSM5602244 r1", "0:100 1:100", "A:1197596235;C:1031109315;G:1057301946;T:1204137304;N:0", 100, 100, null, null, 1197596235, 1031109315, 1057301946, 1204137304, 0, "SRX12388683", "SRS10358684", "SRA1302013", "GEO", "Takaomi Sanda Lab, Cancer Science Institute, National University of Singapore", 1, 0.94477, null, 0.24522, null, 0.74042, null, 0.51025, null, 100, null, "B", null, "usable mapping rate", "bgi", "bgi", "unknown", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "Singapore", "2021-09-28", "Undetermined", "Undetermined", "Cancer or Tumor", "Cancer or Tumor"], [66218, "SRR16102694", "SRX12388682", "SRS10358688", "SRP339154", "PRJNA767083", "RNA seq analysis for IRF4 driven zebrafish tumors post JQ1 treatment", "GSE184946", "Transcriptome Analysis", "RNA seq analysis was performed using tumor cells driven by IRF4 to analyze gene expression changes post JQ1 treatment Overall design: The human IRF4 gene and mCherry fluorescent marker gene were transduced under the zebrafish lck promoter in zebrafish.  post the development of lymphoma phenotype  animals were treated with JQ1 or control DMSO for 1 or 3 days.  Tumor cells were harvested and sorted by flow cytometry based on mCherry fluorescence expression.  Total RNAs were harvested from each sample and then applied for RNA seq analysis.", "parent bioproject:PRJNA701471", "pubmed:35504924", null, "Tumor cells  JQ1 day1 rep1 RNA Seq", "GSM5602243", null, "tissue:Zebrafish tumor cells|cell type:Zebrafish tumor cells|condition:treatment|treatment:JQ1", "Tumor cells  JQ1 day1 rep1 RNA Seq", "RNA seq datasets of DMSO treated control samples and JQ1 treated samples were aligned to the GRCz11 zebrafish genome Ensembl annotation with ERCC spike in information using STAR 2.5.2a with the parameter outFilterMultimapNmax set to 1. featureCount was used for the mapped reads in .bam files to generate count tables based on the Ensembl gene annotation and ERCC annotation. Bioconductor package DESeq2 v1.12.4 was used to analyze differential gene expression using 3 DMSO vs 3 JQ1 treated  samples. Genome build: danRer11 Supplementary files format and content: featureCount read count for all sample  DESeq2 files for differentially expressed genes", "Zebrafish tumor cells", "JQ1 was dissolved in DMSO.  Animals were treated with JQ1 at 4microM or the same amount of DMSO in individual tanks for 1 or 3 days.  Experiments were done using 3 independent animals.", "Total RNA was harvested using the miRNeasy Mini Kit Qiagen.  ERCC spike in was added based on the total cell number. Strand specific library construction and sequencing of paired end  100 bp long reads by the BGISeq500 were performed at the BGI Biotech Solutions Hong Kong Co Ltd Hong Kong.", "The human IRF4 gene and mCherry fluorescent marker gene were transduced under the zebrafish lck promoter in zebrafish.  post the development of lymphoma phenotype  animals were treated with JQ1.   Tumor cells were harvested and sorted by flow cytometry based on mCherry fluorescence expression.  Total RNAs were harvested from each sample and then applied for RNA seq analysis.", "cell type:Zebrafish tumor cells|condition:treatment|treatment:JQ1", "GSM5602243", "GSM5602243: Tumor cells  JQ1 day1 rep1 RNA Seq; Danio rerio; RNA Seq", "GSM5602243", null, "1", "Total RNA was harvested using the miRNeasy Mini Kit Qiagen.  ERCC spike in was added based on the total cell number. Strand specific library construction and sequencing of paired end  100 bp long reads by the BGISeq500 were performed at the BGI Biotech Solutions Hong Kong Co Ltd Hong Kong.", "GEO Accession:GSM5602243", "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "BGISEQ", "BGISEQ-500", null, "SRP339154", null, null, "D1_J1_1.fq.gz D1_J1_2.fq.gz", "fastq fastq", 4434887800.0, 44348878.0, "GSM5602243 r1", "0:100 1:100", "A:1178050199;C:1024865996;G:1046777721;T:1185193884;N:0", 100, 100, null, null, 1178050199, 1024865996, 1046777721, 1185193884, 0, "SRX12388682", "SRS10358688", "SRA1302013", "GEO", "Takaomi Sanda Lab, Cancer Science Institute, National University of Singapore", 1, 0.94501, null, 0.2305, null, 0.76432, null, 0.49472, null, 100, null, "B", null, "usable mapping rate", "bgi", "bgi", "unknown", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "Singapore", "2021-09-28", "Undetermined", "Undetermined", "Cancer or Tumor", "Cancer or Tumor"], [66219, "SRR16102693", "SRX12388681", "SRS10358683", "SRP339154", "PRJNA767083", "RNA seq analysis for IRF4 driven zebrafish tumors post JQ1 treatment", "GSE184946", "Transcriptome Analysis", "RNA seq analysis was performed using tumor cells driven by IRF4 to analyze gene expression changes post JQ1 treatment Overall design: The human IRF4 gene and mCherry fluorescent marker gene were transduced under the zebrafish lck promoter in zebrafish.  post the development of lymphoma phenotype  animals were treated with JQ1 or control DMSO for 1 or 3 days.  Tumor cells were harvested and sorted by flow cytometry based on mCherry fluorescence expression.  Total RNAs were harvested from each sample and then applied for RNA seq analysis.", "parent bioproject:PRJNA701471", "pubmed:35504924", null, "Tumor cells  DMSO day1 rep3 RNA Seq", "GSM5602242", null, "tissue:Zebrafish tumor cells|cell type:Zebrafish tumor cells|condition:control|treatment:DMSO", "Tumor cells  DMSO day1 rep3 RNA Seq", "RNA seq datasets of DMSO treated control samples and JQ1 treated samples were aligned to the GRCz11 zebrafish genome Ensembl annotation with ERCC spike in information using STAR 2.5.2a with the parameter outFilterMultimapNmax set to 1. featureCount was used for the mapped reads in .bam files to generate count tables based on the Ensembl gene annotation and ERCC annotation. Bioconductor package DESeq2 v1.12.4 was used to analyze differential gene expression using 3 DMSO vs 3 JQ1 treated  samples. Genome build: danRer11 Supplementary files format and content: featureCount read count for all sample  DESeq2 files for differentially expressed genes", "Zebrafish tumor cells", "JQ1 was dissolved in DMSO.  Animals were treated with JQ1 at 4microM or the same amount of DMSO in individual tanks for 1 or 3 days.  Experiments were done using 3 independent animals.", "Total RNA was harvested using the miRNeasy Mini Kit Qiagen.  ERCC spike in was added based on the total cell number. Strand specific library construction and sequencing of paired end  100 bp long reads by the BGISeq500 were performed at the BGI Biotech Solutions Hong Kong Co Ltd Hong Kong.", "The human IRF4 gene and mCherry fluorescent marker gene were transduced under the zebrafish lck promoter in zebrafish.  post the development of lymphoma phenotype  animals were treated with JQ1.   Tumor cells were harvested and sorted by flow cytometry based on mCherry fluorescence expression.  Total RNAs were harvested from each sample and then applied for RNA seq analysis.", "cell type:Zebrafish tumor cells|condition:control|treatment:DMSO", "GSM5602242", "GSM5602242: Tumor cells  DMSO day1 rep3 RNA Seq; Danio rerio; RNA Seq", "GSM5602242", null, "1", "Total RNA was harvested using the miRNeasy Mini Kit Qiagen.  ERCC spike in was added based on the total cell number. Strand specific library construction and sequencing of paired end  100 bp long reads by the BGISeq500 were performed at the BGI Biotech Solutions Hong Kong Co Ltd Hong Kong.", "GEO Accession:GSM5602242", "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "BGISEQ", "BGISEQ-500", null, "SRP339154", null, null, "D1_D3_1.fq.gz D1_D3_2.fq.gz", "fastq fastq", 4497882800.0, 44978828.0, "GSM5602242 r1", "0:100 1:100", "A:1193693228;C:1038160916;G:1064460652;T:1201568004;N:0", 100, 100, null, null, 1193693228, 1038160916, 1064460652, 1201568004, 0, "SRX12388681", "SRS10358683", "SRA1302013", "GEO", "Takaomi Sanda Lab, Cancer Science Institute, National University of Singapore", 1, 0.93714, null, 0.19697, null, 0.74554, null, 0.50928, null, 100, null, "B", null, "usable mapping rate", "bgi", "bgi", "unknown", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "Singapore", "2021-09-28", "Undetermined", "Undetermined", "Cancer or Tumor", "Cancer or Tumor"], [66220, "SRR16102692", "SRX12388680", "SRS10358682", "SRP339154", "PRJNA767083", "RNA seq analysis for IRF4 driven zebrafish tumors post JQ1 treatment", "GSE184946", "Transcriptome Analysis", "RNA seq analysis was performed using tumor cells driven by IRF4 to analyze gene expression changes post JQ1 treatment Overall design: The human IRF4 gene and mCherry fluorescent marker gene were transduced under the zebrafish lck promoter in zebrafish.  post the development of lymphoma phenotype  animals were treated with JQ1 or control DMSO for 1 or 3 days.  Tumor cells were harvested and sorted by flow cytometry based on mCherry fluorescence expression.  Total RNAs were harvested from each sample and then applied for RNA seq analysis.", "parent bioproject:PRJNA701471", "pubmed:35504924", null, "Tumor cells  DMSO day1 rep2 RNA Seq", "GSM5602241", null, "tissue:Zebrafish tumor cells|cell type:Zebrafish tumor cells|condition:control|treatment:DMSO", "Tumor cells  DMSO day1 rep2 RNA Seq", "RNA seq datasets of DMSO treated control samples and JQ1 treated samples were aligned to the GRCz11 zebrafish genome Ensembl annotation with ERCC spike in information using STAR 2.5.2a with the parameter outFilterMultimapNmax set to 1. featureCount was used for the mapped reads in .bam files to generate count tables based on the Ensembl gene annotation and ERCC annotation. Bioconductor package DESeq2 v1.12.4 was used to analyze differential gene expression using 3 DMSO vs 3 JQ1 treated  samples. Genome build: danRer11 Supplementary files format and content: featureCount read count for all sample  DESeq2 files for differentially expressed genes", "Zebrafish tumor cells", "JQ1 was dissolved in DMSO.  Animals were treated with JQ1 at 4microM or the same amount of DMSO in individual tanks for 1 or 3 days.  Experiments were done using 3 independent animals.", "Total RNA was harvested using the miRNeasy Mini Kit Qiagen.  ERCC spike in was added based on the total cell number. Strand specific library construction and sequencing of paired end  100 bp long reads by the BGISeq500 were performed at the BGI Biotech Solutions Hong Kong Co Ltd Hong Kong.", "The human IRF4 gene and mCherry fluorescent marker gene were transduced under the zebrafish lck promoter in zebrafish.  post the development of lymphoma phenotype  animals were treated with JQ1.   Tumor cells were harvested and sorted by flow cytometry based on mCherry fluorescence expression.  Total RNAs were harvested from each sample and then applied for RNA seq analysis.", "cell type:Zebrafish tumor cells|condition:control|treatment:DMSO", "GSM5602241", "GSM5602241: Tumor cells  DMSO day1 rep2 RNA Seq; Danio rerio; RNA Seq", "GSM5602241", null, "1", "Total RNA was harvested using the miRNeasy Mini Kit Qiagen.  ERCC spike in was added based on the total cell number. Strand specific library construction and sequencing of paired end  100 bp long reads by the BGISeq500 were performed at the BGI Biotech Solutions Hong Kong Co Ltd Hong Kong.", "GEO Accession:GSM5602241", "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "BGISEQ", "BGISEQ-500", null, "SRP339154", null, null, "D1_D2_1.fq.gz D1_D2_2.fq.gz", "fastq fastq", 4464096200.0, 44640962.0, "GSM5602241 r1", "0:100 1:100", "A:1185692334;C:1031585570;G:1050205768;T:1196612528;N:0", 100, 100, null, null, 1185692334, 1031585570, 1050205768, 1196612528, 0, "SRX12388680", "SRS10358682", "SRA1302013", "GEO", "Takaomi Sanda Lab, Cancer Science Institute, National University of Singapore", 1, 0.94785, null, 0.24425, null, 0.78165, null, 0.4975, null, 100, null, "B", null, "usable mapping rate", "bgi", "bgi", "unknown", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "Singapore", "2021-09-28", "Undetermined", "Undetermined", "Cancer or Tumor", "Cancer or Tumor"], [66221, "SRR16102691", "SRX12388679", "SRS10358681", "SRP339154", "PRJNA767083", "RNA seq analysis for IRF4 driven zebrafish tumors post JQ1 treatment", "GSE184946", "Transcriptome Analysis", "RNA seq analysis was performed using tumor cells driven by IRF4 to analyze gene expression changes post JQ1 treatment Overall design: The human IRF4 gene and mCherry fluorescent marker gene were transduced under the zebrafish lck promoter in zebrafish.  post the development of lymphoma phenotype  animals were treated with JQ1 or control DMSO for 1 or 3 days.  Tumor cells were harvested and sorted by flow cytometry based on mCherry fluorescence expression.  Total RNAs were harvested from each sample and then applied for RNA seq analysis.", "parent bioproject:PRJNA701471", "pubmed:35504924", null, "Tumor cells  DMSO day1 rep1 RNA Seq", "GSM5602240", null, "tissue:Zebrafish tumor cells|cell type:Zebrafish tumor cells|condition:control|treatment:DMSO", "Tumor cells  DMSO day1 rep1 RNA Seq", "RNA seq datasets of DMSO treated control samples and JQ1 treated samples were aligned to the GRCz11 zebrafish genome Ensembl annotation with ERCC spike in information using STAR 2.5.2a with the parameter outFilterMultimapNmax set to 1. featureCount was used for the mapped reads in .bam files to generate count tables based on the Ensembl gene annotation and ERCC annotation. Bioconductor package DESeq2 v1.12.4 was used to analyze differential gene expression using 3 DMSO vs 3 JQ1 treated  samples. Genome build: danRer11 Supplementary files format and content: featureCount read count for all sample  DESeq2 files for differentially expressed genes", "Zebrafish tumor cells", "JQ1 was dissolved in DMSO.  Animals were treated with JQ1 at 4microM or the same amount of DMSO in individual tanks for 1 or 3 days.  Experiments were done using 3 independent animals.", "Total RNA was harvested using the miRNeasy Mini Kit Qiagen.  ERCC spike in was added based on the total cell number. Strand specific library construction and sequencing of paired end  100 bp long reads by the BGISeq500 were performed at the BGI Biotech Solutions Hong Kong Co Ltd Hong Kong.", "The human IRF4 gene and mCherry fluorescent marker gene were transduced under the zebrafish lck promoter in zebrafish.  post the development of lymphoma phenotype  animals were treated with JQ1.   Tumor cells were harvested and sorted by flow cytometry based on mCherry fluorescence expression.  Total RNAs were harvested from each sample and then applied for RNA seq analysis.", "cell type:Zebrafish tumor cells|condition:control|treatment:DMSO", "GSM5602240", "GSM5602240: Tumor cells  DMSO day1 rep1 RNA Seq; Danio rerio; RNA Seq", "GSM5602240", null, "1", "Total RNA was harvested using the miRNeasy Mini Kit Qiagen.  ERCC spike in was added based on the total cell number. Strand specific library construction and sequencing of paired end  100 bp long reads by the BGISeq500 were performed at the BGI Biotech Solutions Hong Kong Co Ltd Hong Kong.", "GEO Accession:GSM5602240", "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "BGISEQ", "BGISEQ-500", null, "SRP339154", null, null, "D1_D1_1.fq.gz D1_D1_2.fq.gz", "fastq fastq", 4466502200.0, 44665022.0, "GSM5602240 r1", "0:100 1:100", "A:1191785539;C:1024344626;G:1050598581;T:1199773454;N:0", 100, 100, null, null, 1191785539, 1024344626, 1050598581, 1199773454, 0, "SRX12388679", "SRS10358681", "SRA1302013", "GEO", "Takaomi Sanda Lab, Cancer Science Institute, National University of Singapore", 1, 0.94073, null, 0.24308, null, 0.77094, null, 0.48493, null, 100, null, "B", null, "usable mapping rate", "bgi", "bgi", "unknown", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "Singapore", "2021-09-28", "Undetermined", "Undetermined", "Cancer or Tumor", "Cancer or Tumor"], [74560, "SRR23829118", "SRX19650818", "SRS17019470", "SRP426991", "PRJNA944150", "Absence of telomerase restricts melanoma development.", "PRJNA944150", "Other", "Most cancers reactivate telomerase to maintain telomere length to acquire immortality. The importance of this process is well illustrated by the fact that telomerase promoter mutations are found at a high frequency in many cancer types  including melanoma. However  it is unclear when and if telomerase is strictly required during tumorigenesis. Here we show that melanoma can occur in the absence of telomerase. Nevertheless  it is required to sustain later growth and avoid regression. Using telomerase mutant zebrafish tert /  combined with two established melanoma models  we found equal melanoma incidence and invasiveness as tumors became visible. Later  however  while WT fish develop increasing larger tumors  tert /  tumors stagnate growth and regress. Consistently  tert /  tumors showed lower cell proliferation  higher apoptosis and melanocyte differentiation. We also detected an immune response to tert /  tumors. Indeed  tert /  tumors resumed growth when transplanted to immunocompromised hosts. We propose that  telomerase is required for melanoma in zebrafish  albeit at later stages of progression  to sustain growth while avoiding immune rejection and regression. Thus  absence of telomerase restricts melanoma through tumor autonomous mechanisms cell cycle arrest  apoptosis and melanocyte differentiation and a non tumor autonomous mechanisms immune rejection.", null, null, null, "3006/10", "7B", null, "strain:AB|age:3 month|sex:male|tissue:Tumor|birth date:10.03.2021|birth location:PEMAV  IRCAN France|death date:30.06.2021|genotype:tert+/+ rep4|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio : 3months male tumor", "7B", "7B", "RNA Seq of Danio rerio : 3months male tumor", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "PAIRED", "BGISEQ", "BGISEQ-500", null, "SRP426991", null, null, "7B_1.fq.gz 7B_2.fq.gz", "fastq fastq", 10796340200.0, 53981701.0, "7B 1.fq.gz", "0:100 1:100", "A:2946489343;C:2411825502;G:2477056291;T:2960969064;N:0", 100, 100, null, null, 2946489343, 2411825502, 2477056291, 2960969064, 0, "SRX19650818", "SRS17019470", "SRA1603485", "Sun Yat-sen University Cancer Center|State Key Laboratory of Oncology in South China", "Sun Yat-sen University Cancer Center Miguel Godinho Ferreira", 2, 0.91639, 0.92098, 0.13624, 0.1358, 0.7443, 0.74367, 0.52593, 0.52158, 100, 100, "B", "B", "biological fallback assumption", "bgi", "bgi", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2023-03-14", "Adult", "Adult", "Cancer or Tumor", "Cancer or Tumor"], [74561, "SRR23829119", "SRX19650817", "SRS17019469", "SRP426991", "PRJNA944150", "Absence of telomerase restricts melanoma development.", "PRJNA944150", "Other", "Most cancers reactivate telomerase to maintain telomere length to acquire immortality. The importance of this process is well illustrated by the fact that telomerase promoter mutations are found at a high frequency in many cancer types  including melanoma. However  it is unclear when and if telomerase is strictly required during tumorigenesis. Here we show that melanoma can occur in the absence of telomerase. Nevertheless  it is required to sustain later growth and avoid regression. Using telomerase mutant zebrafish tert /  combined with two established melanoma models  we found equal melanoma incidence and invasiveness as tumors became visible. Later  however  while WT fish develop increasing larger tumors  tert /  tumors stagnate growth and regress. Consistently  tert /  tumors showed lower cell proliferation  higher apoptosis and melanocyte differentiation. We also detected an immune response to tert /  tumors. Indeed  tert /  tumors resumed growth when transplanted to immunocompromised hosts. We propose that  telomerase is required for melanoma in zebrafish  albeit at later stages of progression  to sustain growth while avoiding immune rejection and regression. Thus  absence of telomerase restricts melanoma through tumor autonomous mechanisms cell cycle arrest  apoptosis and melanocyte differentiation and a non tumor autonomous mechanisms immune rejection.", null, null, null, "3006/12", "8B", null, "strain:AB|age:3 month|sex:male|tissue:Tumor|birth date:10.03.2021|birth location:PEMAV  IRCAN France|death date:30.06.2021|genotype:tert+/+ rep5|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio : 3months male tumor", "8B", "8B", "RNA Seq of Danio rerio : 3months male tumor", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "PAIRED", "BGISEQ", "BGISEQ-500", null, "SRP426991", null, null, "8B_1.fq.gz 8B_2.fq.gz", "fastq fastq", 10560562800.0, 52802814.0, "8B 1.fq.gz", "0:100 1:100", "A:2867789187;C:2366678430;G:2438457525;T:2887637658;N:0", 100, 100, null, null, 2867789187, 2366678430, 2438457525, 2887637658, 0, "SRX19650817", "SRS17019469", "SRA1603485", "Sun Yat-sen University Cancer Center|State Key Laboratory of Oncology in South China", "Sun Yat-sen University Cancer Center Miguel Godinho Ferreira", 2, 0.90976, 0.91391, 0.15248, 0.15201, 0.73499, 0.73379, 0.51572, 0.51882, 100, 100, "B", "B", "biological fallback assumption", "bgi", "bgi", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2023-03-14", "Adult", "Adult", "Cancer or Tumor", "Cancer or Tumor"], [74562, "SRR23829120", "SRX19650816", "SRS17019468", "SRP426991", "PRJNA944150", "Absence of telomerase restricts melanoma development.", "PRJNA944150", "Other", "Most cancers reactivate telomerase to maintain telomere length to acquire immortality. The importance of this process is well illustrated by the fact that telomerase promoter mutations are found at a high frequency in many cancer types  including melanoma. However  it is unclear when and if telomerase is strictly required during tumorigenesis. Here we show that melanoma can occur in the absence of telomerase. Nevertheless  it is required to sustain later growth and avoid regression. Using telomerase mutant zebrafish tert /  combined with two established melanoma models  we found equal melanoma incidence and invasiveness as tumors became visible. Later  however  while WT fish develop increasing larger tumors  tert /  tumors stagnate growth and regress. Consistently  tert /  tumors showed lower cell proliferation  higher apoptosis and melanocyte differentiation. We also detected an immune response to tert /  tumors. Indeed  tert /  tumors resumed growth when transplanted to immunocompromised hosts. We propose that  telomerase is required for melanoma in zebrafish  albeit at later stages of progression  to sustain growth while avoiding immune rejection and regression. Thus  absence of telomerase restricts melanoma through tumor autonomous mechanisms cell cycle arrest  apoptosis and melanocyte differentiation and a non tumor autonomous mechanisms immune rejection.", null, null, null, "2806/1", "9B", null, "strain:AB|age:12 month|sex:male|tissue:Tumor|birth date:24.06.2020|birth location:PEMAV  IRCAN France|death date:28.06.2021|genotype:tert /  rep4|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio : 12 month male tumor", "9B", "9B", "RNA Seq of Danio rerio : 12 month male tumor", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "PAIRED", "BGISEQ", "BGISEQ-500", null, "SRP426991", null, null, "9B_1.fq.gz 9B_2.fq.gz", "fastq fastq", 10805598600.0, 54027993.0, "9B 1.fq.gz", "0:100 1:100", "A:2899169569;C:2457980147;G:2528851393;T:2919597491;N:0", 100, 100, null, null, 2899169569, 2457980147, 2528851393, 2919597491, 0, "SRX19650816", "SRS17019468", "SRA1603485", "Sun Yat-sen University Cancer Center|State Key Laboratory of Oncology in South China", "Sun Yat-sen University Cancer Center Miguel Godinho Ferreira", 2, 0.92637, 0.93052, 0.12486, 0.12507, 0.73352, 0.73188, 0.51498, 0.52101, 100, 100, "B", "B", "biological fallback assumption", "bgi", "bgi", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2023-03-14", "Adult", "Adult", "Cancer or Tumor", "Cancer or Tumor"], [74563, "SRR23829121", "SRX19650815", "SRS17019467", "SRP426991", "PRJNA944150", "Absence of telomerase restricts melanoma development.", "PRJNA944150", "Other", "Most cancers reactivate telomerase to maintain telomere length to acquire immortality. The importance of this process is well illustrated by the fact that telomerase promoter mutations are found at a high frequency in many cancer types  including melanoma. However  it is unclear when and if telomerase is strictly required during tumorigenesis. Here we show that melanoma can occur in the absence of telomerase. Nevertheless  it is required to sustain later growth and avoid regression. Using telomerase mutant zebrafish tert /  combined with two established melanoma models  we found equal melanoma incidence and invasiveness as tumors became visible. Later  however  while WT fish develop increasing larger tumors  tert /  tumors stagnate growth and regress. Consistently  tert /  tumors showed lower cell proliferation  higher apoptosis and melanocyte differentiation. We also detected an immune response to tert /  tumors. Indeed  tert /  tumors resumed growth when transplanted to immunocompromised hosts. We propose that  telomerase is required for melanoma in zebrafish  albeit at later stages of progression  to sustain growth while avoiding immune rejection and regression. Thus  absence of telomerase restricts melanoma through tumor autonomous mechanisms cell cycle arrest  apoptosis and melanocyte differentiation and a non tumor autonomous mechanisms immune rejection.", null, null, null, "2806/2", "10B", null, "strain:AB|age:12 month|sex:male|tissue:Tumor|birth date:24.06.2020|birth location:PEMAV  IRCAN France|death date:28.06.2021|genotype:tert+/+ rep1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio : 12 month male tumor", "10B", "10B", "RNA Seq of Danio rerio : 12 month male tumor", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "PAIRED", "BGISEQ", "BGISEQ-500", null, "SRP426991", null, null, "10B_1.fq.gz 10B_2.fq.gz", "fastq fastq", 10811556000.0, 54057780.0, "10B 1.fq.gz", "0:100 1:100", "A:2898576858;C:2460872365;G:2527128196;T:2924978581;N:0", 100, 100, null, null, 2898576858, 2460872365, 2527128196, 2924978581, 0, "SRX19650815", "SRS17019467", "SRA1603485", "Sun Yat-sen University Cancer Center|State Key Laboratory of Oncology in South China", "Sun Yat-sen University Cancer Center Miguel Godinho Ferreira", 2, 0.92176, 0.92836, 0.12295, 0.12256, 0.72604, 0.72458, 0.51306, 0.51966, 100, 100, "B", "B", "biological fallback assumption", "bgi", "bgi", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2023-03-14", "Adult", "Adult", "Cancer or Tumor", "Cancer or Tumor"], [74564, "SRR23829122", "SRX19650814", "SRS17019466", "SRP426991", "PRJNA944150", "Absence of telomerase restricts melanoma development.", "PRJNA944150", "Other", "Most cancers reactivate telomerase to maintain telomere length to acquire immortality. The importance of this process is well illustrated by the fact that telomerase promoter mutations are found at a high frequency in many cancer types  including melanoma. However  it is unclear when and if telomerase is strictly required during tumorigenesis. Here we show that melanoma can occur in the absence of telomerase. Nevertheless  it is required to sustain later growth and avoid regression. Using telomerase mutant zebrafish tert /  combined with two established melanoma models  we found equal melanoma incidence and invasiveness as tumors became visible. Later  however  while WT fish develop increasing larger tumors  tert /  tumors stagnate growth and regress. Consistently  tert /  tumors showed lower cell proliferation  higher apoptosis and melanocyte differentiation. We also detected an immune response to tert /  tumors. Indeed  tert /  tumors resumed growth when transplanted to immunocompromised hosts. We propose that  telomerase is required for melanoma in zebrafish  albeit at later stages of progression  to sustain growth while avoiding immune rejection and regression. Thus  absence of telomerase restricts melanoma through tumor autonomous mechanisms cell cycle arrest  apoptosis and melanocyte differentiation and a non tumor autonomous mechanisms immune rejection.", null, null, null, "3006/9", "6B", null, "strain:AB|age:3 month|sex:male|tissue:Tumor|birth date:10.03.2021|birth location:PEMAV  IRCAN France|death date:30.06.2021|genotype:tert+/+ rep3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio : 3months male tumor", "6B", "6B", "RNA Seq of Danio rerio : 3months male tumor", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "PAIRED", "BGISEQ", "BGISEQ-500", null, "SRP426991", null, null, "6B_1.fq.gz 6B_2.fq.gz", "fastq fastq", 10809952000.0, 54049760.0, "6B 1.fq.gz", "0:100 1:100", "A:2931041183;C:2429723251;G:2502283305;T:2946904261;N:0", 100, 100, null, null, 2931041183, 2429723251, 2502283305, 2946904261, 0, "SRX19650814", "SRS17019466", "SRA1603485", "Sun Yat-sen University Cancer Center|State Key Laboratory of Oncology in South China", "Sun Yat-sen University Cancer Center Miguel Godinho Ferreira", 2, 0.92179, 0.92775, 0.13661, 0.1365, 0.74679, 0.74556, 0.52477, 0.52471, 100, 100, "B", "B", "biological fallback assumption", "bgi", "bgi", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2023-03-14", "Adult", "Adult", "Cancer or Tumor", "Cancer or Tumor"], [74565, "SRR23829123", "SRX19650813", "SRS17019465", "SRP426991", "PRJNA944150", "Absence of telomerase restricts melanoma development.", "PRJNA944150", "Other", "Most cancers reactivate telomerase to maintain telomere length to acquire immortality. The importance of this process is well illustrated by the fact that telomerase promoter mutations are found at a high frequency in many cancer types  including melanoma. However  it is unclear when and if telomerase is strictly required during tumorigenesis. Here we show that melanoma can occur in the absence of telomerase. Nevertheless  it is required to sustain later growth and avoid regression. Using telomerase mutant zebrafish tert /  combined with two established melanoma models  we found equal melanoma incidence and invasiveness as tumors became visible. Later  however  while WT fish develop increasing larger tumors  tert /  tumors stagnate growth and regress. Consistently  tert /  tumors showed lower cell proliferation  higher apoptosis and melanocyte differentiation. We also detected an immune response to tert /  tumors. Indeed  tert /  tumors resumed growth when transplanted to immunocompromised hosts. We propose that  telomerase is required for melanoma in zebrafish  albeit at later stages of progression  to sustain growth while avoiding immune rejection and regression. Thus  absence of telomerase restricts melanoma through tumor autonomous mechanisms cell cycle arrest  apoptosis and melanocyte differentiation and a non tumor autonomous mechanisms immune rejection.", null, null, null, "3006/8", "5B", null, "strain:AB|age:3 month|sex:male|tissue:Tumor|birth date:10.03.2021|birth location:PEMAV  IRCAN France|death date:30.06.2021|genotype:tert+/+ rep2|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio : 3months male tumor", "5B", "5B", "RNA Seq of Danio rerio : 3months male tumor", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "PAIRED", "BGISEQ", "BGISEQ-500", null, "SRP426991", null, null, "5B_1.fq.gz 5B_2.fq.gz", "fastq fastq", 10604822000.0, 53024110.0, "5B 1.fq.gz", "0:100 1:100", "A:2847359136;C:2411906169;G:2481911629;T:2863645066;N:0", 100, 100, null, null, 2847359136, 2411906169, 2481911629, 2863645066, 0, "SRX19650813", "SRS17019465", "SRA1603485", "Sun Yat-sen University Cancer Center|State Key Laboratory of Oncology in South China", "Sun Yat-sen University Cancer Center Miguel Godinho Ferreira", 2, 0.92231, 0.92771, 0.13302, 0.13258, 0.74052, 0.73843, 0.51718, 0.51163, 100, 100, "B", "B", "biological fallback assumption", "bgi", "bgi", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2023-03-14", "Adult", "Adult", "Cancer or Tumor", "Cancer or Tumor"], [74566, "SRR23829124", "SRX19650812", "SRS17019464", "SRP426991", "PRJNA944150", "Absence of telomerase restricts melanoma development.", "PRJNA944150", "Other", "Most cancers reactivate telomerase to maintain telomere length to acquire immortality. The importance of this process is well illustrated by the fact that telomerase promoter mutations are found at a high frequency in many cancer types  including melanoma. However  it is unclear when and if telomerase is strictly required during tumorigenesis. Here we show that melanoma can occur in the absence of telomerase. Nevertheless  it is required to sustain later growth and avoid regression. Using telomerase mutant zebrafish tert /  combined with two established melanoma models  we found equal melanoma incidence and invasiveness as tumors became visible. Later  however  while WT fish develop increasing larger tumors  tert /  tumors stagnate growth and regress. Consistently  tert /  tumors showed lower cell proliferation  higher apoptosis and melanocyte differentiation. We also detected an immune response to tert /  tumors. Indeed  tert /  tumors resumed growth when transplanted to immunocompromised hosts. We propose that  telomerase is required for melanoma in zebrafish  albeit at later stages of progression  to sustain growth while avoiding immune rejection and regression. Thus  absence of telomerase restricts melanoma through tumor autonomous mechanisms cell cycle arrest  apoptosis and melanocyte differentiation and a non tumor autonomous mechanisms immune rejection.", null, null, null, "3006/7", "4B", null, "strain:AB|age:3 month|sex:male|tissue:Tumor|birth date:10.03.2021|birth location:PEMAV  IRCAN France|death date:30.06.2021|genotype:tert+/+ rep1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio : 3months male tumor", "4B", "4B", "RNA Seq of Danio rerio : 3months male tumor", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "PAIRED", "BGISEQ", "BGISEQ-500", null, "SRP426991", null, null, "4B_1.fq.gz 4B_2.fq.gz", "fastq fastq", 10792140600.0, 53960703.0, "4B 1.fq.gz", "0:100 1:100", "A:2882693797;C:2464129073;G:2535344124;T:2909973606;N:0", 100, 100, null, null, 2882693797, 2464129073, 2535344124, 2909973606, 0, "SRX19650812", "SRS17019464", "SRA1603485", "Sun Yat-sen University Cancer Center|State Key Laboratory of Oncology in South China", "Sun Yat-sen University Cancer Center Miguel Godinho Ferreira", 2, 0.91884, 0.92345, 0.12967, 0.12884, 0.74117, 0.74103, 0.50792, 0.51065, 100, 100, "B", "B", "biological fallback assumption", "bgi", "bgi", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2023-03-14", "Adult", "Adult", "Cancer or Tumor", "Cancer or Tumor"], [74567, "SRR23829125", "SRX19650811", "SRS17019463", "SRP426991", "PRJNA944150", "Absence of telomerase restricts melanoma development.", "PRJNA944150", "Other", "Most cancers reactivate telomerase to maintain telomere length to acquire immortality. The importance of this process is well illustrated by the fact that telomerase promoter mutations are found at a high frequency in many cancer types  including melanoma. However  it is unclear when and if telomerase is strictly required during tumorigenesis. Here we show that melanoma can occur in the absence of telomerase. Nevertheless  it is required to sustain later growth and avoid regression. Using telomerase mutant zebrafish tert /  combined with two established melanoma models  we found equal melanoma incidence and invasiveness as tumors became visible. Later  however  while WT fish develop increasing larger tumors  tert /  tumors stagnate growth and regress. Consistently  tert /  tumors showed lower cell proliferation  higher apoptosis and melanocyte differentiation. We also detected an immune response to tert /  tumors. Indeed  tert /  tumors resumed growth when transplanted to immunocompromised hosts. We propose that  telomerase is required for melanoma in zebrafish  albeit at later stages of progression  to sustain growth while avoiding immune rejection and regression. Thus  absence of telomerase restricts melanoma through tumor autonomous mechanisms cell cycle arrest  apoptosis and melanocyte differentiation and a non tumor autonomous mechanisms immune rejection.", null, null, null, "3006/4", "3B", null, "strain:AB|age:3 month|sex:male|tissue:Tumor|birth date:10.03.2021|birth location:PEMAV  IRCAN France|death date:30.06.2021|genotype:tert /  rep3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio : 3months male tumor", "3B", "3B", "RNA Seq of Danio rerio : 3months male tumor", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "PAIRED", "BGISEQ", "BGISEQ-500", null, "SRP426991", null, null, "3B_1.fq.gz 3B_2.fq.gz", "fastq fastq", 10817072400.0, 54085362.0, "3B 1.fq.gz", "0:100 1:100", "A:2980754877;C:2390812269;G:2448674730;T:2996830524;N:0", 100, 100, null, null, 2980754877, 2390812269, 2448674730, 2996830524, 0, "SRX19650811", "SRS17019463", "SRA1603485", "Sun Yat-sen University Cancer Center|State Key Laboratory of Oncology in South China", "Sun Yat-sen University Cancer Center Miguel Godinho Ferreira", 2, 0.91015, 0.9166, 0.15854, 0.15694, 0.73724, 0.73442, 0.5243, 0.52496, 100, 100, "B", "B", "biological fallback assumption", "bgi", "bgi", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2023-03-14", "Adult", "Adult", "Cancer or Tumor", "Cancer or Tumor"], [74568, "SRR23829126", "SRX19650810", "SRS17019462", "SRP426991", "PRJNA944150", "Absence of telomerase restricts melanoma development.", "PRJNA944150", "Other", "Most cancers reactivate telomerase to maintain telomere length to acquire immortality. The importance of this process is well illustrated by the fact that telomerase promoter mutations are found at a high frequency in many cancer types  including melanoma. However  it is unclear when and if telomerase is strictly required during tumorigenesis. Here we show that melanoma can occur in the absence of telomerase. Nevertheless  it is required to sustain later growth and avoid regression. Using telomerase mutant zebrafish tert /  combined with two established melanoma models  we found equal melanoma incidence and invasiveness as tumors became visible. Later  however  while WT fish develop increasing larger tumors  tert /  tumors stagnate growth and regress. Consistently  tert /  tumors showed lower cell proliferation  higher apoptosis and melanocyte differentiation. We also detected an immune response to tert /  tumors. Indeed  tert /  tumors resumed growth when transplanted to immunocompromised hosts. We propose that  telomerase is required for melanoma in zebrafish  albeit at later stages of progression  to sustain growth while avoiding immune rejection and regression. Thus  absence of telomerase restricts melanoma through tumor autonomous mechanisms cell cycle arrest  apoptosis and melanocyte differentiation and a non tumor autonomous mechanisms immune rejection.", null, null, null, "2104/14", "19B", null, "strain:AB|age:10 month|sex:male|tissue:Tumor|birth date:24.06.2020|birth location:PEMAV  IRCAN France|death date:21.04.2021|genotype:tert /  rep3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio : 10 month male tumor", "19B", "19B", "RNA Seq of Danio rerio : 10 month male tumor", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "PAIRED", "BGISEQ", "BGISEQ-500", null, "SRP426991", null, null, "19B_1.fq.gz 19B_2.fq.gz", "fastq fastq", 12603676000.0, 63018380.0, "19B 1.fq.gz", "0:100 1:100", "A:3414935292;C:2859787251;G:2916851430;T:3412102027;N:0", 100, 100, null, null, 3414935292, 2859787251, 2916851430, 3412102027, 0, "SRX19650810", "SRS17019462", "SRA1603485", "Sun Yat-sen University Cancer Center|State Key Laboratory of Oncology in South China", "Sun Yat-sen University Cancer Center Miguel Godinho Ferreira", 2, 0.92494, 0.92995, 0.12064, 0.12054, 0.73807, 0.73649, 0.52554, 0.52564, 100, 100, "B", "B", "biological fallback assumption", "bgi", "bgi", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2023-03-14", "Adult", "Adult", "Cancer or Tumor", "Cancer or Tumor"], [74569, "SRR23829127", "SRX19650809", "SRS17019461", "SRP426991", "PRJNA944150", "Absence of telomerase restricts melanoma development.", "PRJNA944150", "Other", "Most cancers reactivate telomerase to maintain telomere length to acquire immortality. The importance of this process is well illustrated by the fact that telomerase promoter mutations are found at a high frequency in many cancer types  including melanoma. However  it is unclear when and if telomerase is strictly required during tumorigenesis. Here we show that melanoma can occur in the absence of telomerase. Nevertheless  it is required to sustain later growth and avoid regression. Using telomerase mutant zebrafish tert /  combined with two established melanoma models  we found equal melanoma incidence and invasiveness as tumors became visible. Later  however  while WT fish develop increasing larger tumors  tert /  tumors stagnate growth and regress. Consistently  tert /  tumors showed lower cell proliferation  higher apoptosis and melanocyte differentiation. We also detected an immune response to tert /  tumors. Indeed  tert /  tumors resumed growth when transplanted to immunocompromised hosts. We propose that  telomerase is required for melanoma in zebrafish  albeit at later stages of progression  to sustain growth while avoiding immune rejection and regression. Thus  absence of telomerase restricts melanoma through tumor autonomous mechanisms cell cycle arrest  apoptosis and melanocyte differentiation and a non tumor autonomous mechanisms immune rejection.", null, null, null, "2104/9", "18B", null, "strain:AB|age:10 month|sex:male|tissue:Tumor|birth date:24.06.2020|birth location:PEMAV  IRCAN France|death date:21.04.2021|genotype:tert /  rep2|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio : 10 month male tumor", "18B", "18B", "RNA Seq of Danio rerio : 10 month male tumor", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "PAIRED", "BGISEQ", "BGISEQ-500", null, "SRP426991", null, null, "18B_1.fq.gz 18B_2.fq.gz", "fastq fastq", 12562625800.0, 62813129.0, "18B 1.fq.gz", "0:100 1:100", "A:3435125429;C:2818006678;G:2880078673;T:3429415020;N:0", 100, 100, null, null, 3435125429, 2818006678, 2880078673, 3429415020, 0, "SRX19650809", "SRS17019461", "SRA1603485", "Sun Yat-sen University Cancer Center|State Key Laboratory of Oncology in South China", "Sun Yat-sen University Cancer Center Miguel Godinho Ferreira", 2, 0.92476, 0.92851, 0.12505, 0.12384, 0.74961, 0.74882, 0.52843, 0.52817, 100, 100, "B", "B", "biological fallback assumption", "bgi", "bgi", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2023-03-14", "Adult", "Adult", "Cancer or Tumor", "Cancer or Tumor"], [74570, "SRR23829128", "SRX19650808", "SRS17019460", "SRP426991", "PRJNA944150", "Absence of telomerase restricts melanoma development.", "PRJNA944150", "Other", "Most cancers reactivate telomerase to maintain telomere length to acquire immortality. The importance of this process is well illustrated by the fact that telomerase promoter mutations are found at a high frequency in many cancer types  including melanoma. However  it is unclear when and if telomerase is strictly required during tumorigenesis. Here we show that melanoma can occur in the absence of telomerase. Nevertheless  it is required to sustain later growth and avoid regression. Using telomerase mutant zebrafish tert /  combined with two established melanoma models  we found equal melanoma incidence and invasiveness as tumors became visible. Later  however  while WT fish develop increasing larger tumors  tert /  tumors stagnate growth and regress. Consistently  tert /  tumors showed lower cell proliferation  higher apoptosis and melanocyte differentiation. We also detected an immune response to tert /  tumors. Indeed  tert /  tumors resumed growth when transplanted to immunocompromised hosts. We propose that  telomerase is required for melanoma in zebrafish  albeit at later stages of progression  to sustain growth while avoiding immune rejection and regression. Thus  absence of telomerase restricts melanoma through tumor autonomous mechanisms cell cycle arrest  apoptosis and melanocyte differentiation and a non tumor autonomous mechanisms immune rejection.", null, null, null, "2104/1", "17B", null, "strain:AB|age:10 month|sex:male|tissue:Tumor|birth date:24.06.2020|birth location:PEMAV  IRCAN France|death date:21.04.2021|genotype:tert+/+ rep5|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio : 10 month male tumor", "17B", "17B", "RNA Seq of Danio rerio : 10 month male tumor", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "PAIRED", "BGISEQ", "BGISEQ-500", null, "SRP426991", null, null, "17B_1.fq.gz 17B_2.fq.gz", "fastq fastq", 8787148400.0, 43935742.0, "17B 1.fq.gz", "0:100 1:100", "A:2617726378;C:1773416208;G:1829194025;T:2566811789;N:0", 100, 100, null, null, 2617726378, 1773416208, 1829194025, 2566811789, 0, "SRX19650808", "SRS17019460", "SRA1603485", "Sun Yat-sen University Cancer Center|State Key Laboratory of Oncology in South China", "Sun Yat-sen University Cancer Center Miguel Godinho Ferreira", 2, 0.84629, 0.85164, 0.49471, 0.49585, 0.79545, 0.79275, 0.64785, 0.57659, 100, 100, "B", "B", "biological fallback assumption", "bgi", "bgi", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2023-03-14", "Adult", "Adult", "Cancer or Tumor", "Cancer or Tumor"], [74571, "SRR23829129", "SRX19650807", "SRS17019459", "SRP426991", "PRJNA944150", "Absence of telomerase restricts melanoma development.", "PRJNA944150", "Other", "Most cancers reactivate telomerase to maintain telomere length to acquire immortality. The importance of this process is well illustrated by the fact that telomerase promoter mutations are found at a high frequency in many cancer types  including melanoma. However  it is unclear when and if telomerase is strictly required during tumorigenesis. Here we show that melanoma can occur in the absence of telomerase. Nevertheless  it is required to sustain later growth and avoid regression. Using telomerase mutant zebrafish tert /  combined with two established melanoma models  we found equal melanoma incidence and invasiveness as tumors became visible. Later  however  while WT fish develop increasing larger tumors  tert /  tumors stagnate growth and regress. Consistently  tert /  tumors showed lower cell proliferation  higher apoptosis and melanocyte differentiation. We also detected an immune response to tert /  tumors. Indeed  tert /  tumors resumed growth when transplanted to immunocompromised hosts. We propose that  telomerase is required for melanoma in zebrafish  albeit at later stages of progression  to sustain growth while avoiding immune rejection and regression. Thus  absence of telomerase restricts melanoma through tumor autonomous mechanisms cell cycle arrest  apoptosis and melanocyte differentiation and a non tumor autonomous mechanisms immune rejection.", null, null, null, "2806/3", "16B", null, "strain:AB|age:12 month|sex:male|tissue:Tumor|birth date:24.06.2020|birth location:PEMAV  IRCAN France|death date:28.06.2021|genotype:tert+/+ rep4|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio : 12 month male tumor", "16B", "16B", "RNA Seq of Danio rerio : 12 month male tumor", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "PAIRED", "BGISEQ", "BGISEQ-500", null, "SRP426991", null, null, "16B_1.fq.gz 16B_2.fq.gz", "fastq fastq", 12372317000.0, 61861585.0, "16B 1.fq.gz", "0:100 1:100", "A:3325923130;C:2824746317;G:2889873754;T:3331773799;N:0", 100, 100, null, null, 3325923130, 2824746317, 2889873754, 3331773799, 0, "SRX19650807", "SRS17019459", "SRA1603485", "Sun Yat-sen University Cancer Center|State Key Laboratory of Oncology in South China", "Sun Yat-sen University Cancer Center Miguel Godinho Ferreira", 2, 0.92023, 0.92579, 0.13379, 0.13406, 0.75974, 0.75954, 0.51721, 0.53155, 100, 100, "B", "B", "biological fallback assumption", "bgi", "bgi", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2023-03-14", "Adult", "Adult", "Cancer or Tumor", "Cancer or Tumor"], [74572, "SRR23829130", "SRX19650806", "SRS17019458", "SRP426991", "PRJNA944150", "Absence of telomerase restricts melanoma development.", "PRJNA944150", "Other", "Most cancers reactivate telomerase to maintain telomere length to acquire immortality. The importance of this process is well illustrated by the fact that telomerase promoter mutations are found at a high frequency in many cancer types  including melanoma. However  it is unclear when and if telomerase is strictly required during tumorigenesis. Here we show that melanoma can occur in the absence of telomerase. Nevertheless  it is required to sustain later growth and avoid regression. Using telomerase mutant zebrafish tert /  combined with two established melanoma models  we found equal melanoma incidence and invasiveness as tumors became visible. Later  however  while WT fish develop increasing larger tumors  tert /  tumors stagnate growth and regress. Consistently  tert /  tumors showed lower cell proliferation  higher apoptosis and melanocyte differentiation. We also detected an immune response to tert /  tumors. Indeed  tert /  tumors resumed growth when transplanted to immunocompromised hosts. We propose that  telomerase is required for melanoma in zebrafish  albeit at later stages of progression  to sustain growth while avoiding immune rejection and regression. Thus  absence of telomerase restricts melanoma through tumor autonomous mechanisms cell cycle arrest  apoptosis and melanocyte differentiation and a non tumor autonomous mechanisms immune rejection.", null, null, null, "2506/2", "15B", null, "strain:AB|age:12 month|sex:male|tissue:Tumor|birth date:24.06.2020|birth location:PEMAV  IRCAN France|death date:25.06.2021|genotype:tert+/+ rep3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio : 12 month male tumor", "15B", "15B", "RNA Seq of Danio rerio : 12 month male tumor", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "PAIRED", "BGISEQ", "BGISEQ-500", null, "SRP426991", null, null, "15B_1.fq.gz 15B_2.fq.gz", "fastq fastq", 12111580000.0, 60557900.0, "15B 1.fq.gz", "0:100 1:100", "A:3253473957;C:2781714853;G:2829572138;T:3246819052;N:0", 100, 100, null, null, 3253473957, 2781714853, 2829572138, 3246819052, 0, "SRX19650806", "SRS17019458", "SRA1603485", "Sun Yat-sen University Cancer Center|State Key Laboratory of Oncology in South China", "Sun Yat-sen University Cancer Center Miguel Godinho Ferreira", 2, 0.92909, 0.9333, 0.12994, 0.13008, 0.76997, 0.76921, 0.53473, 0.52603, 100, 100, "B", "B", "biological fallback assumption", "bgi", "bgi", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2023-03-14", "Adult", "Adult", "Cancer or Tumor", "Cancer or Tumor"], [74573, "SRR23829131", "SRX19650805", "SRS17019457", "SRP426991", "PRJNA944150", "Absence of telomerase restricts melanoma development.", "PRJNA944150", "Other", "Most cancers reactivate telomerase to maintain telomere length to acquire immortality. The importance of this process is well illustrated by the fact that telomerase promoter mutations are found at a high frequency in many cancer types  including melanoma. However  it is unclear when and if telomerase is strictly required during tumorigenesis. Here we show that melanoma can occur in the absence of telomerase. Nevertheless  it is required to sustain later growth and avoid regression. Using telomerase mutant zebrafish tert /  combined with two established melanoma models  we found equal melanoma incidence and invasiveness as tumors became visible. Later  however  while WT fish develop increasing larger tumors  tert /  tumors stagnate growth and regress. Consistently  tert /  tumors showed lower cell proliferation  higher apoptosis and melanocyte differentiation. We also detected an immune response to tert /  tumors. Indeed  tert /  tumors resumed growth when transplanted to immunocompromised hosts. We propose that  telomerase is required for melanoma in zebrafish  albeit at later stages of progression  to sustain growth while avoiding immune rejection and regression. Thus  absence of telomerase restricts melanoma through tumor autonomous mechanisms cell cycle arrest  apoptosis and melanocyte differentiation and a non tumor autonomous mechanisms immune rejection.", null, null, null, "2506/1", "14B", null, "strain:AB|age:12 month|sex:male|tissue:Tumor|birth date:24.06.2020|birth location:PEMAV  IRCAN France|death date:25.06.2021|genotype:tert /  rep1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio : 12 month male tumor", "14B", "14B", "RNA Seq of Danio rerio : 12 month male tumor", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "PAIRED", "BGISEQ", "BGISEQ-500", null, "SRP426991", null, null, "14B_1.fq.gz 14B_2.fq.gz", "fastq fastq", 12314492000.0, 61572460.0, "14B 1.fq.gz", "0:100 1:100", "A:3364570176;C:2760126284;G:2813170851;T:3376624689;N:0", 100, 100, null, null, 3364570176, 2760126284, 2813170851, 3376624689, 0, "SRX19650805", "SRS17019457", "SRA1603485", "Sun Yat-sen University Cancer Center|State Key Laboratory of Oncology in South China", "Sun Yat-sen University Cancer Center Miguel Godinho Ferreira", 2, 0.92824, 0.93249, 0.16314, 0.16227, 0.74217, 0.74142, 0.54103, 0.54082, 100, 100, "B", "B", "biological fallback assumption", "bgi", "bgi", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2023-03-14", "Adult", "Adult", "Cancer or Tumor", "Cancer or Tumor"], [74574, "SRR23829132", "SRX19650804", "SRS17019456", "SRP426991", "PRJNA944150", "Absence of telomerase restricts melanoma development.", "PRJNA944150", "Other", "Most cancers reactivate telomerase to maintain telomere length to acquire immortality. The importance of this process is well illustrated by the fact that telomerase promoter mutations are found at a high frequency in many cancer types  including melanoma. However  it is unclear when and if telomerase is strictly required during tumorigenesis. Here we show that melanoma can occur in the absence of telomerase. Nevertheless  it is required to sustain later growth and avoid regression. Using telomerase mutant zebrafish tert /  combined with two established melanoma models  we found equal melanoma incidence and invasiveness as tumors became visible. Later  however  while WT fish develop increasing larger tumors  tert /  tumors stagnate growth and regress. Consistently  tert /  tumors showed lower cell proliferation  higher apoptosis and melanocyte differentiation. We also detected an immune response to tert /  tumors. Indeed  tert /  tumors resumed growth when transplanted to immunocompromised hosts. We propose that  telomerase is required for melanoma in zebrafish  albeit at later stages of progression  to sustain growth while avoiding immune rejection and regression. Thus  absence of telomerase restricts melanoma through tumor autonomous mechanisms cell cycle arrest  apoptosis and melanocyte differentiation and a non tumor autonomous mechanisms immune rejection.", null, null, null, "3006/11", "13B", null, "strain:AB|age:3 month|sex:male|tissue:Tumor|birth date:10.03.2021|birth location:PEMAV  IRCAN France|death date:30.06.2021|genotype:tert+/+ rep6|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio : 3months male tumor", "13B", "13B", "RNA Seq of Danio rerio : 3months male tumor", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "PAIRED", "BGISEQ", "BGISEQ-500", null, "SRP426991", null, null, "13B_1.fq.gz 13B_2.fq.gz", "fastq fastq", 12281192200.0, 61405961.0, "13B 1.fq.gz", "0:100 1:100", "A:3369650885;C:2745871807;G:2821246280;T:3344423228;N:0", 100, 100, null, null, 3369650885, 2745871807, 2821246280, 3344423228, 0, "SRX19650804", "SRS17019456", "SRA1603485", "Sun Yat-sen University Cancer Center|State Key Laboratory of Oncology in South China", "Sun Yat-sen University Cancer Center Miguel Godinho Ferreira", 2, 0.93143, 0.93303, 0.1814, 0.18252, 0.76207, 0.76169, 0.55108, 0.54553, 100, 100, "B", "B", "biological fallback assumption", "bgi", "bgi", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2023-03-14", "Adult", "Adult", "Cancer or Tumor", "Cancer or Tumor"], [74575, "SRR23829133", "SRX19650803", "SRS17019455", "SRP426991", "PRJNA944150", "Absence of telomerase restricts melanoma development.", "PRJNA944150", "Other", "Most cancers reactivate telomerase to maintain telomere length to acquire immortality. The importance of this process is well illustrated by the fact that telomerase promoter mutations are found at a high frequency in many cancer types  including melanoma. However  it is unclear when and if telomerase is strictly required during tumorigenesis. Here we show that melanoma can occur in the absence of telomerase. Nevertheless  it is required to sustain later growth and avoid regression. Using telomerase mutant zebrafish tert /  combined with two established melanoma models  we found equal melanoma incidence and invasiveness as tumors became visible. Later  however  while WT fish develop increasing larger tumors  tert /  tumors stagnate growth and regress. Consistently  tert /  tumors showed lower cell proliferation  higher apoptosis and melanocyte differentiation. We also detected an immune response to tert /  tumors. Indeed  tert /  tumors resumed growth when transplanted to immunocompromised hosts. We propose that  telomerase is required for melanoma in zebrafish  albeit at later stages of progression  to sustain growth while avoiding immune rejection and regression. Thus  absence of telomerase restricts melanoma through tumor autonomous mechanisms cell cycle arrest  apoptosis and melanocyte differentiation and a non tumor autonomous mechanisms immune rejection.", null, null, null, "3006/2", "12B", null, "strain:AB|age:3 month|sex:male|tissue:Tumor|birth date:10.03.2021|birth location:PEMAV  IRCAN France|death date:30.06.2021|genotype:tert /  rep4|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio : 3months male tumor", "12B", "12B", "RNA Seq of Danio rerio : 3months male tumor", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "PAIRED", "BGISEQ", "BGISEQ-500", null, "SRP426991", null, null, "12B_1.fq.gz 12B_2.fq.gz", "fastq fastq", 12163842800.0, 60819214.0, "12B 1.fq.gz", "0:100 1:100", "A:3431206764;C:2629189354;G:2672190281;T:3431256401;N:0", 100, 100, null, null, 3431206764, 2629189354, 2672190281, 3431256401, 0, "SRX19650803", "SRS17019455", "SRA1603485", "Sun Yat-sen University Cancer Center|State Key Laboratory of Oncology in South China", "Sun Yat-sen University Cancer Center Miguel Godinho Ferreira", 2, 0.91204, 0.91933, 0.22594, 0.22625, 0.73777, 0.73586, 0.5046, 0.47081, 100, 100, "B", "B", "biological fallback assumption", "bgi", "bgi", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2023-03-14", "Adult", "Adult", "Cancer or Tumor", "Cancer or Tumor"], [74576, "SRR23829134", "SRX19650802", "SRS17019454", "SRP426991", "PRJNA944150", "Absence of telomerase restricts melanoma development.", "PRJNA944150", "Other", "Most cancers reactivate telomerase to maintain telomere length to acquire immortality. The importance of this process is well illustrated by the fact that telomerase promoter mutations are found at a high frequency in many cancer types  including melanoma. However  it is unclear when and if telomerase is strictly required during tumorigenesis. Here we show that melanoma can occur in the absence of telomerase. Nevertheless  it is required to sustain later growth and avoid regression. Using telomerase mutant zebrafish tert /  combined with two established melanoma models  we found equal melanoma incidence and invasiveness as tumors became visible. Later  however  while WT fish develop increasing larger tumors  tert /  tumors stagnate growth and regress. Consistently  tert /  tumors showed lower cell proliferation  higher apoptosis and melanocyte differentiation. We also detected an immune response to tert /  tumors. Indeed  tert /  tumors resumed growth when transplanted to immunocompromised hosts. We propose that  telomerase is required for melanoma in zebrafish  albeit at later stages of progression  to sustain growth while avoiding immune rejection and regression. Thus  absence of telomerase restricts melanoma through tumor autonomous mechanisms cell cycle arrest  apoptosis and melanocyte differentiation and a non tumor autonomous mechanisms immune rejection.", null, null, null, "2806/4", "11B", null, "strain:AB|age:12 month|sex:male|tissue:Tumor|birth date:24.06.2020|birth location:PEMAV  IRCAN France|death date:28.06.2021|genotype:tert+/+ rep2|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio : 12 month male tumor", "11B", "11B", "RNA Seq of Danio rerio : 12 month male tumor", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "PAIRED", "BGISEQ", "BGISEQ-500", null, "SRP426991", null, null, "11B_1.fq.gz 11B_2.fq.gz", "fastq fastq", 10583058000.0, 52915290.0, "11B 1.fq.gz", "0:100 1:100", "A:2970647072;C:2293859072;G:2357521919;T:2961029937;N:0", 100, 100, null, null, 2970647072, 2293859072, 2357521919, 2961029937, 0, "SRX19650802", "SRS17019454", "SRA1603485", "Sun Yat-sen University Cancer Center|State Key Laboratory of Oncology in South China", "Sun Yat-sen University Cancer Center Miguel Godinho Ferreira", 2, 0.90787, 0.91228, 0.1916, 0.19016, 0.72271, 0.72092, 0.52084, 0.52228, 100, 100, "B", "B", "biological fallback assumption", "bgi", "bgi", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2023-03-14", "Adult", "Adult", "Cancer or Tumor", "Cancer or Tumor"], [74577, "SRR23829135", "SRX19650801", "SRS17019453", "SRP426991", "PRJNA944150", "Absence of telomerase restricts melanoma development.", "PRJNA944150", "Other", "Most cancers reactivate telomerase to maintain telomere length to acquire immortality. The importance of this process is well illustrated by the fact that telomerase promoter mutations are found at a high frequency in many cancer types  including melanoma. However  it is unclear when and if telomerase is strictly required during tumorigenesis. Here we show that melanoma can occur in the absence of telomerase. Nevertheless  it is required to sustain later growth and avoid regression. Using telomerase mutant zebrafish tert /  combined with two established melanoma models  we found equal melanoma incidence and invasiveness as tumors became visible. Later  however  while WT fish develop increasing larger tumors  tert /  tumors stagnate growth and regress. Consistently  tert /  tumors showed lower cell proliferation  higher apoptosis and melanocyte differentiation. We also detected an immune response to tert /  tumors. Indeed  tert /  tumors resumed growth when transplanted to immunocompromised hosts. We propose that  telomerase is required for melanoma in zebrafish  albeit at later stages of progression  to sustain growth while avoiding immune rejection and regression. Thus  absence of telomerase restricts melanoma through tumor autonomous mechanisms cell cycle arrest  apoptosis and melanocyte differentiation and a non tumor autonomous mechanisms immune rejection.", null, null, null, "3006/3", "2B", null, "strain:AB|age:3 month|sex:male|tissue:Tumor|birth date:10.03.2021|birth location:PEMAV  IRCAN France|death date:30.06.2021|genotype:tert /  rep2|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio : 3months male tumor", "2B", "2B", "RNA Seq of Danio rerio : 3months male tumor", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "PAIRED", "BGISEQ", "BGISEQ-500", null, "SRP426991", null, null, "2B_1.fq.gz 2B_2.fq.gz", "fastq fastq", 10776715000.0, 53883575.0, "2B 1.fq.gz", "0:100 1:100", "A:2948670507;C:2400698003;G:2461964006;T:2965382484;N:0", 100, 100, null, null, 2948670507, 2400698003, 2461964006, 2965382484, 0, "SRX19650801", "SRS17019453", "SRA1603485", "Sun Yat-sen University Cancer Center|State Key Laboratory of Oncology in South China", "Sun Yat-sen University Cancer Center Miguel Godinho Ferreira", 2, 0.91594, 0.91925, 0.17372, 0.17392, 0.73708, 0.73742, 0.52748, 0.53599, 100, 100, "B", "B", "biological fallback assumption", "bgi", "bgi", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2023-03-14", "Adult", "Adult", "Cancer or Tumor", "Cancer or Tumor"], [74578, "SRR23829136", "SRX19650800", "SRS17019452", "SRP426991", "PRJNA944150", "Absence of telomerase restricts melanoma development.", "PRJNA944150", "Other", "Most cancers reactivate telomerase to maintain telomere length to acquire immortality. The importance of this process is well illustrated by the fact that telomerase promoter mutations are found at a high frequency in many cancer types  including melanoma. However  it is unclear when and if telomerase is strictly required during tumorigenesis. Here we show that melanoma can occur in the absence of telomerase. Nevertheless  it is required to sustain later growth and avoid regression. Using telomerase mutant zebrafish tert /  combined with two established melanoma models  we found equal melanoma incidence and invasiveness as tumors became visible. Later  however  while WT fish develop increasing larger tumors  tert /  tumors stagnate growth and regress. Consistently  tert /  tumors showed lower cell proliferation  higher apoptosis and melanocyte differentiation. We also detected an immune response to tert /  tumors. Indeed  tert /  tumors resumed growth when transplanted to immunocompromised hosts. We propose that  telomerase is required for melanoma in zebrafish  albeit at later stages of progression  to sustain growth while avoiding immune rejection and regression. Thus  absence of telomerase restricts melanoma through tumor autonomous mechanisms cell cycle arrest  apoptosis and melanocyte differentiation and a non tumor autonomous mechanisms immune rejection.", null, null, null, "3006/1", "1B", null, "strain:AB|age:3 month|sex:male|tissue:Tumor|birth date:10.03.2021|birth location:PEMAV  IRCAN France|death date:30.06.2021|genotype:tert /  rep1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio : 3months male tumor", "1B", "1B", "RNA Seq of Danio rerio : 3months male tumor", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "PAIRED", "BGISEQ", "BGISEQ-500", null, "SRP426991", null, null, "1B_1.fq.gz 1B_2.fq.gz", "fastq fastq", 10556248400.0, 52781242.0, "1B 1.fq.gz", "0:100 1:100", "A:2849865226;C:2384848330;G:2457353526;T:2864181318;N:0", 100, 100, null, null, 2849865226, 2384848330, 2457353526, 2864181318, 0, "SRX19650800", "SRS17019452", "SRA1603485", "Sun Yat-sen University Cancer Center|State Key Laboratory of Oncology in South China", "Sun Yat-sen University Cancer Center Miguel Godinho Ferreira", 2, 0.91378, 0.91775, 0.13203, 0.13044, 0.73373, 0.73342, 0.51084, 0.51194, 100, 100, "B", "B", "biological fallback assumption", "bgi", "bgi", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2023-03-14", "Adult", "Adult", "Cancer or Tumor", "Cancer or Tumor"]], "truncated": false, "filtered_table_rows_count": 31, "expanded_columns": [], "expandable_columns": [], "columns": ["rowid", "run.accession", "experiment.accession", "sample.accession", "study.accession", "bioproject", "study.title", "study.alias", "study.type", "study.abstract", "study.attributes", "study.PMIDs", "sample.description", "sample.title", "sample.alias", "sample.centername", "sample.attributes", "GEOsample.title", "GEOsample.dataprocessing", "GEOsample.source", "GEOsample.treatmentprotocol", "GEOsample.extractprotocol", "GEOsample.growthprotocol", "GEOsample.characteristics", "GEOsample.accession", "experiment.title", "experiment.alias", "experiment.library_name", "experiment.design_description", "experiment.library_construction_protocol", "experiment.attributes", "experiment.library_strategy", "experiment.library_source", "experiment.library_selection", "experiment.library_layout", "experiment.platform", "experiment.instrument_model", "experiment.spot_descriptor", "experiment.study_ref", "run.title", "run.attributes", "run.filename", "run.semantic_name", "run.total_bases", "run.total_spots", "run.alias", "run.read_lengths", "run.base_counts", "run.r1_length", "run.r2_length", "run.r3_length", "run.r4_length", "run.Acount", "run.Ccount", "run.Gcount", "run.Tcount", "run.Ncount", "run.experiment", "run.pool_member", "submission.accession", "submission.srasource", "submission.bioprojectsource", "seqdetective.n_mates", "seqdetective.mapping_rate.mate1", "seqdetective.mapping_rate.mate2", "seqdetective.nofeature_rate.mate1", "seqdetective.nofeature_rate.mate2", "seqdetective.sparsity.mate1", "seqdetective.sparsity.mate2", "seqdetective.pos_strand_rate.mate1", "seqdetective.pos_strand_rate.mate2", "seqdetective.readlen.mate1", "seqdetective.readlen.mate2", "seqdetective.judgement.mate1", "seqdetective.judgement.mate2", "seqdetective.judgement.reason", "platform_family", "instrument_generation", "read_bias", "selection_class", "prep_kit", "sc_or_bulk", "tech_class", "technology", "tech_variant", "submission.bioprojectsource.country", "earliest_date", "devstage_curation", "devstage_curation_coarse", "tissue_curation", "tissue_curation_coarse"], "primary_keys": [], "units": {}, "query": {"sql": "select rowid, [run.accession], [experiment.accession], [sample.accession], [study.accession], bioproject, [study.title], [study.alias], [study.type], [study.abstract], [study.attributes], [study.PMIDs], [sample.description], [sample.title], [sample.alias], [sample.centername], [sample.attributes], [GEOsample.title], [GEOsample.dataprocessing], [GEOsample.source], [GEOsample.treatmentprotocol], [GEOsample.extractprotocol], [GEOsample.growthprotocol], [GEOsample.characteristics], [GEOsample.accession], [experiment.title], [experiment.alias], [experiment.library_name], [experiment.design_description], [experiment.library_construction_protocol], [experiment.attributes], [experiment.library_strategy], [experiment.library_source], [experiment.library_selection], [experiment.library_layout], [experiment.platform], [experiment.instrument_model], [experiment.spot_descriptor], [experiment.study_ref], [run.title], [run.attributes], [run.filename], [run.semantic_name], [run.total_bases], [run.total_spots], [run.alias], [run.read_lengths], [run.base_counts], [run.r1_length], [run.r2_length], [run.r3_length], [run.r4_length], [run.Acount], [run.Ccount], [run.Gcount], [run.Tcount], [run.Ncount], [run.experiment], [run.pool_member], [submission.accession], [submission.srasource], [submission.bioprojectsource], [seqdetective.n_mates], [seqdetective.mapping_rate.mate1], [seqdetective.mapping_rate.mate2], [seqdetective.nofeature_rate.mate1], [seqdetective.nofeature_rate.mate2], [seqdetective.sparsity.mate1], [seqdetective.sparsity.mate2], [seqdetective.pos_strand_rate.mate1], [seqdetective.pos_strand_rate.mate2], [seqdetective.readlen.mate1], [seqdetective.readlen.mate2], [seqdetective.judgement.mate1], [seqdetective.judgement.mate2], [seqdetective.judgement.reason], platform_family, instrument_generation, read_bias, selection_class, prep_kit, sc_or_bulk, tech_class, technology, tech_variant, [submission.bioprojectsource.country], earliest_date, devstage_curation, devstage_curation_coarse, tissue_curation, tissue_curation_coarse from run_metadata where \"experiment.library_strategy\" = :p0 and \"experiment.platform\" = :p1 and \"tissue_curation\" = :p2 order by rowid limit 101", "params": {"p0": "RNA-Seq", "p1": "BGISEQ", "p2": "Cancer or Tumor"}}, "facet_results": 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