{"database": "metadata", "table": "run_metadata", "is_view": false, "human_description_en": "where experiment.library_source = \"TRANSCRIPTOMIC SINGLE CELL\" and tissue_curation_coarse = \"Liver and Biliary System\"", "rows": [[33210, "SRR29868290", "SRX25365557", "SRS22031297", "SRP520597", "PRJNA1136957", "Cholangiocytes contribute to hepatocyte regeneration post partial liver injury in larval zebrafish", "GSE272484", "Transcriptome Analysis", "Liver possesses robust regenerative ability  characterized by flexibility in the cellular source of regeneration based on the extent of the injury. post partial hepatectomy or minor injuries  hepatocytes  the primary liver cells  undergo self duplication to replenish the liver mass. In contrast  when the damage is extensive  or hepatocyte proliferation is impaired  cholangiocytes contribute to hepatocyte recovery. This current paradigm of regenerative flexibility in the liver has been established for animals with little or no growth. However  the regenerative mechanisms during periods of growth in young animals remain unexplored. Here  we establish two new partial liver injury protocols in the zebrafish model of rapid growth during late larval stage and observe emergence of de novo hepatocytes in the presence of spared hepatocytes. Using single cell RNA sequencing and lineage tracing  we identify cholangiocytes as the source of de novo hepatocytes. Our study offers a new perspective on the current paradigm of liver regenerating by proposing cholangiocyte to hepatocyte transdifferentiation as the default mechanism of hepatocyte recovery in late larval stage zebrafish. Overall design: We conducted single cell RNA Sequencing using the 10x platform of larval zebrafish liver. This was performed in the basal state Control1  Control2  post partial ablation MTZ 0dppa  MTZ 1dppa and MTZ 9dppa and post partial hepatectomy PHx 4dpi and PHx 11dpi. The two control samples were not treated with 4 OHT  and were also not injured. The control samples are from 13 dpf zebrafish raised under normal rearing condition. For all experiments Tgfabp10a:CellCousin line was used.", null, "pubmed:40480975", null, "Liver PHx 11dpi", "GSM8403320", null, "source name:Liver|tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Partial Hepatectomy|geo loc name:missing|collection date:missing", "Liver PHx 11dpi", "A custom Cell Ranger index was generated from the Ensembl GRCz11 genome sequence and annotation filtered with \u2018mkgtf\u2019 command of Cell Ranger options: \u2018\u2014attribute = gene biotype:lincRNA \u2013attribute = gene biotype:antisense  as well as the pseudo mTagBFP2  H2B mGL  mCherry NTR sequences in these cells using the mkref command. This index was used to map reads and generate gene expression matrices using the \u2018count\u2019 command of the Cell Ranger software v.7.1.0 provided by 10x Genomics with the option \u201c\u2013include  introns\u201d set to False all other options were used as per default. Assembly: GRCz11 Supplementary files format and content: Count Matrix as h5ad file Supplementary files format and content: Metadata listing the cell annotations as csv file", "Liver", null, "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer\u2019s instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell 3\u2019 v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer\u2019s protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Partial Hepatectomy", "GSM8403320", "GSM8403320: Liver PHx 11dpi; Danio rerio; RNA Seq", "GSM8403320 r1", "GSM8403320", "1", "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer's instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell three prime v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer's protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 550", null, "SRP520597", null, "loader:fastq load.py", "ULB-CB-cr-zf-EES2-SEE-PHx-11dpi-GEM-11-04-24_S24_L001_R2_001.fastq.gz ULB-CB-cr-zf-EES2-SEE-PHx-11dpi-GEM-11-04-24_S24_L001_R1_001.fastq.gz ULB-CB-cr-zf-EES2-SEE-PHx-11dpi-GEM-11-04-24_S24_L001_I2_001.fastq.gz ULB-CB-cr-zf-EES2-SEE-PHx-11dpi-GEM-11-04-24_S24_L001_I1_001.fastq.gz", "fastq fastq fastq fastq", 14533781976.0, 86510607.0, "GSM8403320 r1", null, null, null, null, null, null, null, null, null, null, null, "SRX25365557", "SRS22031297", "SRA1926595", "Regeneration and Stress Biology, IRIBHM, ULB", "Regeneration and Stress Biology, IRIBHM, ULB", 1, 0.92123, null, 0.07848, null, 0.87767, null, 0.63307, null, 120, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "Belgium", "2024-07-17", "Undetermined", "Larval", "Liver", "Liver and Biliary System"], [33211, "SRR29868291", "SRX25365557", "SRS22031297", "SRP520597", "PRJNA1136957", "Cholangiocytes contribute to hepatocyte regeneration post partial liver injury in larval zebrafish", "GSE272484", "Transcriptome Analysis", "Liver possesses robust regenerative ability  characterized by flexibility in the cellular source of regeneration based on the extent of the injury. post partial hepatectomy or minor injuries  hepatocytes  the primary liver cells  undergo self duplication to replenish the liver mass. In contrast  when the damage is extensive  or hepatocyte proliferation is impaired  cholangiocytes contribute to hepatocyte recovery. This current paradigm of regenerative flexibility in the liver has been established for animals with little or no growth. However  the regenerative mechanisms during periods of growth in young animals remain unexplored. Here  we establish two new partial liver injury protocols in the zebrafish model of rapid growth during late larval stage and observe emergence of de novo hepatocytes in the presence of spared hepatocytes. Using single cell RNA sequencing and lineage tracing  we identify cholangiocytes as the source of de novo hepatocytes. Our study offers a new perspective on the current paradigm of liver regenerating by proposing cholangiocyte to hepatocyte transdifferentiation as the default mechanism of hepatocyte recovery in late larval stage zebrafish. Overall design: We conducted single cell RNA Sequencing using the 10x platform of larval zebrafish liver. This was performed in the basal state Control1  Control2  post partial ablation MTZ 0dppa  MTZ 1dppa and MTZ 9dppa and post partial hepatectomy PHx 4dpi and PHx 11dpi. The two control samples were not treated with 4 OHT  and were also not injured. The control samples are from 13 dpf zebrafish raised under normal rearing condition. For all experiments Tgfabp10a:CellCousin line was used.", null, "pubmed:40480975", null, "Liver PHx 11dpi", "GSM8403320", null, "source name:Liver|tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Partial Hepatectomy|geo loc name:missing|collection date:missing", "Liver PHx 11dpi", "A custom Cell Ranger index was generated from the Ensembl GRCz11 genome sequence and annotation filtered with \u2018mkgtf\u2019 command of Cell Ranger options: \u2018\u2014attribute = gene biotype:lincRNA \u2013attribute = gene biotype:antisense  as well as the pseudo mTagBFP2  H2B mGL  mCherry NTR sequences in these cells using the mkref command. This index was used to map reads and generate gene expression matrices using the \u2018count\u2019 command of the Cell Ranger software v.7.1.0 provided by 10x Genomics with the option \u201c\u2013include  introns\u201d set to False all other options were used as per default. Assembly: GRCz11 Supplementary files format and content: Count Matrix as h5ad file Supplementary files format and content: Metadata listing the cell annotations as csv file", "Liver", null, "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer\u2019s instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell 3\u2019 v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer\u2019s protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Partial Hepatectomy", "GSM8403320", "GSM8403320: Liver PHx 11dpi; Danio rerio; RNA Seq", "GSM8403320 r1", "GSM8403320", "1", "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer's instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell three prime v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer's protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 550", null, "SRP520597", null, "loader:fastq load.py", "ULB-CB-cr-zf-EES2-SEE-PHx-11dpi-GEM-11-04-24_S24_L002_R2_001.fastq.gz ULB-CB-cr-zf-EES2-SEE-PHx-11dpi-GEM-11-04-24_S24_L002_R1_001.fastq.gz ULB-CB-cr-zf-EES2-SEE-PHx-11dpi-GEM-11-04-24_S24_L002_I2_001.fastq.gz ULB-CB-cr-zf-EES2-SEE-PHx-11dpi-GEM-11-04-24_S24_L002_I1_001.fastq.gz", "fastq fastq fastq fastq", 14988315888.0, 89216166.0, "GSM8403320 r2", "0:10 1:10 2:28 3:120", "A:3248904317;C:2406836003;G:2506118019;T:2544017722;N:63859", 10, 10, 28, 120, 3248904317, 2406836003, 2506118019, 2544017722, 63859, "SRX25365557", "SRS22031297", "SRA1926595", "Regeneration and Stress Biology, IRIBHM, ULB", "Regeneration and Stress Biology, IRIBHM, ULB", 1, 0.92258, null, 0.07901, null, 0.87805, null, 0.61529, null, 120, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "Belgium", "2024-07-17", "Undetermined", "Larval", "Liver", "Liver and Biliary System"], [33212, "SRR29868292", "SRX25365557", "SRS22031297", "SRP520597", "PRJNA1136957", "Cholangiocytes contribute to hepatocyte regeneration post partial liver injury in larval zebrafish", "GSE272484", "Transcriptome Analysis", "Liver possesses robust regenerative ability  characterized by flexibility in the cellular source of regeneration based on the extent of the injury. post partial hepatectomy or minor injuries  hepatocytes  the primary liver cells  undergo self duplication to replenish the liver mass. In contrast  when the damage is extensive  or hepatocyte proliferation is impaired  cholangiocytes contribute to hepatocyte recovery. This current paradigm of regenerative flexibility in the liver has been established for animals with little or no growth. However  the regenerative mechanisms during periods of growth in young animals remain unexplored. Here  we establish two new partial liver injury protocols in the zebrafish model of rapid growth during late larval stage and observe emergence of de novo hepatocytes in the presence of spared hepatocytes. Using single cell RNA sequencing and lineage tracing  we identify cholangiocytes as the source of de novo hepatocytes. Our study offers a new perspective on the current paradigm of liver regenerating by proposing cholangiocyte to hepatocyte transdifferentiation as the default mechanism of hepatocyte recovery in late larval stage zebrafish. Overall design: We conducted single cell RNA Sequencing using the 10x platform of larval zebrafish liver. This was performed in the basal state Control1  Control2  post partial ablation MTZ 0dppa  MTZ 1dppa and MTZ 9dppa and post partial hepatectomy PHx 4dpi and PHx 11dpi. The two control samples were not treated with 4 OHT  and were also not injured. The control samples are from 13 dpf zebrafish raised under normal rearing condition. For all experiments Tgfabp10a:CellCousin line was used.", null, "pubmed:40480975", null, "Liver PHx 11dpi", "GSM8403320", null, "source name:Liver|tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Partial Hepatectomy|geo loc name:missing|collection date:missing", "Liver PHx 11dpi", "A custom Cell Ranger index was generated from the Ensembl GRCz11 genome sequence and annotation filtered with \u2018mkgtf\u2019 command of Cell Ranger options: \u2018\u2014attribute = gene biotype:lincRNA \u2013attribute = gene biotype:antisense  as well as the pseudo mTagBFP2  H2B mGL  mCherry NTR sequences in these cells using the mkref command. This index was used to map reads and generate gene expression matrices using the \u2018count\u2019 command of the Cell Ranger software v.7.1.0 provided by 10x Genomics with the option \u201c\u2013include  introns\u201d set to False all other options were used as per default. Assembly: GRCz11 Supplementary files format and content: Count Matrix as h5ad file Supplementary files format and content: Metadata listing the cell annotations as csv file", "Liver", null, "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer\u2019s instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell 3\u2019 v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer\u2019s protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Partial Hepatectomy", "GSM8403320", "GSM8403320: Liver PHx 11dpi; Danio rerio; RNA Seq", "GSM8403320 r1", "GSM8403320", "1", "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer's instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell three prime v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer's protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 550", null, "SRP520597", null, "loader:fastq load.py", "ULB-CB-cr-zf-EES2-SEE-PHx-11dpi-GEM-11-04-24_S24_L003_R2_001.fastq.gz ULB-CB-cr-zf-EES2-SEE-PHx-11dpi-GEM-11-04-24_S24_L003_R1_001.fastq.gz ULB-CB-cr-zf-EES2-SEE-PHx-11dpi-GEM-11-04-24_S24_L003_I2_001.fastq.gz ULB-CB-cr-zf-EES2-SEE-PHx-11dpi-GEM-11-04-24_S24_L003_I1_001.fastq.gz", "fastq fastq fastq fastq", 15113782152.0, 89962989.0, "GSM8403320 r3", "0:10 1:10 2:28 3:120", "A:3272277396;C:2428985319;G:2529710055;T:2564489719;N:96191", 10, 10, 28, 120, 3272277396, 2428985319, 2529710055, 2564489719, 96191, "SRX25365557", "SRS22031297", "SRA1926595", "Regeneration and Stress Biology, IRIBHM, ULB", "Regeneration and Stress Biology, IRIBHM, ULB", 1, 0.92256, null, 0.07848, null, 0.87815, null, 0.62057, null, 120, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "Belgium", "2024-07-17", "Undetermined", "Larval", "Liver", "Liver and Biliary System"], [33213, "SRR29868293", "SRX25365557", "SRS22031297", "SRP520597", "PRJNA1136957", "Cholangiocytes contribute to hepatocyte regeneration post partial liver injury in larval zebrafish", "GSE272484", "Transcriptome Analysis", "Liver possesses robust regenerative ability  characterized by flexibility in the cellular source of regeneration based on the extent of the injury. post partial hepatectomy or minor injuries  hepatocytes  the primary liver cells  undergo self duplication to replenish the liver mass. In contrast  when the damage is extensive  or hepatocyte proliferation is impaired  cholangiocytes contribute to hepatocyte recovery. This current paradigm of regenerative flexibility in the liver has been established for animals with little or no growth. However  the regenerative mechanisms during periods of growth in young animals remain unexplored. Here  we establish two new partial liver injury protocols in the zebrafish model of rapid growth during late larval stage and observe emergence of de novo hepatocytes in the presence of spared hepatocytes. Using single cell RNA sequencing and lineage tracing  we identify cholangiocytes as the source of de novo hepatocytes. Our study offers a new perspective on the current paradigm of liver regenerating by proposing cholangiocyte to hepatocyte transdifferentiation as the default mechanism of hepatocyte recovery in late larval stage zebrafish. Overall design: We conducted single cell RNA Sequencing using the 10x platform of larval zebrafish liver. This was performed in the basal state Control1  Control2  post partial ablation MTZ 0dppa  MTZ 1dppa and MTZ 9dppa and post partial hepatectomy PHx 4dpi and PHx 11dpi. The two control samples were not treated with 4 OHT  and were also not injured. The control samples are from 13 dpf zebrafish raised under normal rearing condition. For all experiments Tgfabp10a:CellCousin line was used.", null, "pubmed:40480975", null, "Liver PHx 11dpi", "GSM8403320", null, "source name:Liver|tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Partial Hepatectomy|geo loc name:missing|collection date:missing", "Liver PHx 11dpi", "A custom Cell Ranger index was generated from the Ensembl GRCz11 genome sequence and annotation filtered with \u2018mkgtf\u2019 command of Cell Ranger options: \u2018\u2014attribute = gene biotype:lincRNA \u2013attribute = gene biotype:antisense  as well as the pseudo mTagBFP2  H2B mGL  mCherry NTR sequences in these cells using the mkref command. This index was used to map reads and generate gene expression matrices using the \u2018count\u2019 command of the Cell Ranger software v.7.1.0 provided by 10x Genomics with the option \u201c\u2013include  introns\u201d set to False all other options were used as per default. Assembly: GRCz11 Supplementary files format and content: Count Matrix as h5ad file Supplementary files format and content: Metadata listing the cell annotations as csv file", "Liver", null, "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer\u2019s instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell 3\u2019 v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer\u2019s protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Partial Hepatectomy", "GSM8403320", "GSM8403320: Liver PHx 11dpi; Danio rerio; RNA Seq", "GSM8403320 r1", "GSM8403320", "1", "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer's instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell three prime v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer's protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 550", null, "SRP520597", null, "loader:fastq load.py", "ULB-CB-cr-zf-EES2-SEE-PHx-11dpi-GEM-11-04-24_S24_L004_R2_001.fastq.gz ULB-CB-cr-zf-EES2-SEE-PHx-11dpi-GEM-11-04-24_S24_L004_R1_001.fastq.gz ULB-CB-cr-zf-EES2-SEE-PHx-11dpi-GEM-11-04-24_S24_L004_I2_001.fastq.gz ULB-CB-cr-zf-EES2-SEE-PHx-11dpi-GEM-11-04-24_S24_L004_I1_001.fastq.gz", "fastq fastq fastq fastq", 14872072488.0, 88524241.0, "GSM8403320 r4", "0:10 1:10 2:28 3:120", "A:3227423261;C:2385566347;G:2480919292;T:2528894334;N:105686", 10, 10, 28, 120, 3227423261, 2385566347, 2480919292, 2528894334, 105686, "SRX25365557", "SRS22031297", "SRA1926595", "Regeneration and Stress Biology, IRIBHM, ULB", "Regeneration and Stress Biology, IRIBHM, ULB", 1, 0.92066, null, 0.07821, null, 0.87941, null, 0.63528, null, 120, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "Belgium", "2024-07-17", "Undetermined", "Larval", "Liver", "Liver and Biliary System"], [33214, "SRR29868294", "SRX25365556", "SRS22031296", "SRP520597", "PRJNA1136957", "Cholangiocytes contribute to hepatocyte regeneration post partial liver injury in larval zebrafish", "GSE272484", "Transcriptome Analysis", "Liver possesses robust regenerative ability  characterized by flexibility in the cellular source of regeneration based on the extent of the injury. post partial hepatectomy or minor injuries  hepatocytes  the primary liver cells  undergo self duplication to replenish the liver mass. In contrast  when the damage is extensive  or hepatocyte proliferation is impaired  cholangiocytes contribute to hepatocyte recovery. This current paradigm of regenerative flexibility in the liver has been established for animals with little or no growth. However  the regenerative mechanisms during periods of growth in young animals remain unexplored. Here  we establish two new partial liver injury protocols in the zebrafish model of rapid growth during late larval stage and observe emergence of de novo hepatocytes in the presence of spared hepatocytes. Using single cell RNA sequencing and lineage tracing  we identify cholangiocytes as the source of de novo hepatocytes. Our study offers a new perspective on the current paradigm of liver regenerating by proposing cholangiocyte to hepatocyte transdifferentiation as the default mechanism of hepatocyte recovery in late larval stage zebrafish. Overall design: We conducted single cell RNA Sequencing using the 10x platform of larval zebrafish liver. This was performed in the basal state Control1  Control2  post partial ablation MTZ 0dppa  MTZ 1dppa and MTZ 9dppa and post partial hepatectomy PHx 4dpi and PHx 11dpi. The two control samples were not treated with 4 OHT  and were also not injured. The control samples are from 13 dpf zebrafish raised under normal rearing condition. For all experiments Tgfabp10a:CellCousin line was used.", null, "pubmed:40480975", null, "Liver PHx 4dpi", "GSM8403319", null, "source name:Liver|tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Partial Hepatectomy|geo loc name:missing|collection date:missing", "Liver PHx 4dpi", "A custom Cell Ranger index was generated from the Ensembl GRCz11 genome sequence and annotation filtered with \u2018mkgtf\u2019 command of Cell Ranger options: \u2018\u2014attribute = gene biotype:lincRNA \u2013attribute = gene biotype:antisense  as well as the pseudo mTagBFP2  H2B mGL  mCherry NTR sequences in these cells using the mkref command. This index was used to map reads and generate gene expression matrices using the \u2018count\u2019 command of the Cell Ranger software v.7.1.0 provided by 10x Genomics with the option \u201c\u2013include  introns\u201d set to False all other options were used as per default. Assembly: GRCz11 Supplementary files format and content: Count Matrix as h5ad file Supplementary files format and content: Metadata listing the cell annotations as csv file", "Liver", null, "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer\u2019s instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell 3\u2019 v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer\u2019s protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Partial Hepatectomy", "GSM8403319", "GSM8403319: Liver PHx 4dpi; Danio rerio; RNA Seq", "GSM8403319 r1", "GSM8403319", "1", "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer's instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell three prime v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer's protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 550", null, "SRP520597", null, "loader:fastq load.py", "ULB-CB-cr-zf-EES1-SEE-BBNTR-PHx-4dpi-GEM-04-04-24_S23_L001_I1_001.fastq.gz ULB-CB-cr-zf-EES1-SEE-BBNTR-PHx-4dpi-GEM-04-04-24_S23_L001_I2_001.fastq.gz ULB-CB-cr-zf-EES1-SEE-BBNTR-PHx-4dpi-GEM-04-04-24_S23_L001_R1_001.fastq.gz ULB-CB-cr-zf-EES1-SEE-BBNTR-PHx-4dpi-GEM-04-04-24_S23_L001_R2_001.fastq.gz", "fastq fastq fastq fastq", 17814930504.0, 106041253.0, "GSM8403319 r1", "0:10 1:10 2:28 3:120", "A:3778122253;C:2833144305;G:2888284601;T:3225338000;N:61201", 10, 10, 28, 120, 3778122253, 2833144305, 2888284601, 3225338000, 61201, "SRX25365556", "SRS22031296", "SRA1926595", "Regeneration and Stress Biology, IRIBHM, ULB", "Regeneration and Stress Biology, IRIBHM, ULB", 1, 0.92579, null, 0.07861, null, 0.82518, null, 0.61746, null, 120, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "Belgium", "2024-07-17", "Undetermined", "Larval", "Liver", "Liver and Biliary System"], [33215, "SRR29868295", "SRX25365556", "SRS22031296", "SRP520597", "PRJNA1136957", "Cholangiocytes contribute to hepatocyte regeneration post partial liver injury in larval zebrafish", "GSE272484", "Transcriptome Analysis", "Liver possesses robust regenerative ability  characterized by flexibility in the cellular source of regeneration based on the extent of the injury. post partial hepatectomy or minor injuries  hepatocytes  the primary liver cells  undergo self duplication to replenish the liver mass. In contrast  when the damage is extensive  or hepatocyte proliferation is impaired  cholangiocytes contribute to hepatocyte recovery. This current paradigm of regenerative flexibility in the liver has been established for animals with little or no growth. However  the regenerative mechanisms during periods of growth in young animals remain unexplored. Here  we establish two new partial liver injury protocols in the zebrafish model of rapid growth during late larval stage and observe emergence of de novo hepatocytes in the presence of spared hepatocytes. Using single cell RNA sequencing and lineage tracing  we identify cholangiocytes as the source of de novo hepatocytes. Our study offers a new perspective on the current paradigm of liver regenerating by proposing cholangiocyte to hepatocyte transdifferentiation as the default mechanism of hepatocyte recovery in late larval stage zebrafish. Overall design: We conducted single cell RNA Sequencing using the 10x platform of larval zebrafish liver. This was performed in the basal state Control1  Control2  post partial ablation MTZ 0dppa  MTZ 1dppa and MTZ 9dppa and post partial hepatectomy PHx 4dpi and PHx 11dpi. The two control samples were not treated with 4 OHT  and were also not injured. The control samples are from 13 dpf zebrafish raised under normal rearing condition. For all experiments Tgfabp10a:CellCousin line was used.", null, "pubmed:40480975", null, "Liver PHx 4dpi", "GSM8403319", null, "source name:Liver|tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Partial Hepatectomy|geo loc name:missing|collection date:missing", "Liver PHx 4dpi", "A custom Cell Ranger index was generated from the Ensembl GRCz11 genome sequence and annotation filtered with \u2018mkgtf\u2019 command of Cell Ranger options: \u2018\u2014attribute = gene biotype:lincRNA \u2013attribute = gene biotype:antisense  as well as the pseudo mTagBFP2  H2B mGL  mCherry NTR sequences in these cells using the mkref command. This index was used to map reads and generate gene expression matrices using the \u2018count\u2019 command of the Cell Ranger software v.7.1.0 provided by 10x Genomics with the option \u201c\u2013include  introns\u201d set to False all other options were used as per default. Assembly: GRCz11 Supplementary files format and content: Count Matrix as h5ad file Supplementary files format and content: Metadata listing the cell annotations as csv file", "Liver", null, "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer\u2019s instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell 3\u2019 v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer\u2019s protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Partial Hepatectomy", "GSM8403319", "GSM8403319: Liver PHx 4dpi; Danio rerio; RNA Seq", "GSM8403319 r1", "GSM8403319", "1", "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer's instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell three prime v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer's protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 550", null, "SRP520597", null, "loader:fastq load.py", "ULB-CB-cr-zf-EES1-SEE-BBNTR-PHx-4dpi-GEM-04-04-24_S23_L002_I1_001.fastq.gz ULB-CB-cr-zf-EES1-SEE-BBNTR-PHx-4dpi-GEM-04-04-24_S23_L002_I2_001.fastq.gz ULB-CB-cr-zf-EES1-SEE-BBNTR-PHx-4dpi-GEM-04-04-24_S23_L002_R1_001.fastq.gz ULB-CB-cr-zf-EES1-SEE-BBNTR-PHx-4dpi-GEM-04-04-24_S23_L002_R2_001.fastq.gz", "fastq fastq fastq fastq", 18469195752.0, 109935689.0, "GSM8403319 r2", "0:10 1:10 2:28 3:120", "A:3912835271;C:2940938740;G:3000841815;T:3337590023;N:76831", 10, 10, 28, 120, 3912835271, 2940938740, 3000841815, 3337590023, 76831, "SRX25365556", "SRS22031296", "SRA1926595", "Regeneration and Stress Biology, IRIBHM, ULB", "Regeneration and Stress Biology, IRIBHM, ULB", 1, 0.92613, null, 0.07975, null, 0.82599, null, 0.6186, null, 120, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "Belgium", "2024-07-17", "Undetermined", "Larval", "Liver", "Liver and Biliary System"], [33216, "SRR29868296", "SRX25365556", "SRS22031296", "SRP520597", "PRJNA1136957", "Cholangiocytes contribute to hepatocyte regeneration post partial liver injury in larval zebrafish", "GSE272484", "Transcriptome Analysis", "Liver possesses robust regenerative ability  characterized by flexibility in the cellular source of regeneration based on the extent of the injury. post partial hepatectomy or minor injuries  hepatocytes  the primary liver cells  undergo self duplication to replenish the liver mass. In contrast  when the damage is extensive  or hepatocyte proliferation is impaired  cholangiocytes contribute to hepatocyte recovery. This current paradigm of regenerative flexibility in the liver has been established for animals with little or no growth. However  the regenerative mechanisms during periods of growth in young animals remain unexplored. Here  we establish two new partial liver injury protocols in the zebrafish model of rapid growth during late larval stage and observe emergence of de novo hepatocytes in the presence of spared hepatocytes. Using single cell RNA sequencing and lineage tracing  we identify cholangiocytes as the source of de novo hepatocytes. Our study offers a new perspective on the current paradigm of liver regenerating by proposing cholangiocyte to hepatocyte transdifferentiation as the default mechanism of hepatocyte recovery in late larval stage zebrafish. Overall design: We conducted single cell RNA Sequencing using the 10x platform of larval zebrafish liver. This was performed in the basal state Control1  Control2  post partial ablation MTZ 0dppa  MTZ 1dppa and MTZ 9dppa and post partial hepatectomy PHx 4dpi and PHx 11dpi. The two control samples were not treated with 4 OHT  and were also not injured. The control samples are from 13 dpf zebrafish raised under normal rearing condition. For all experiments Tgfabp10a:CellCousin line was used.", null, "pubmed:40480975", null, "Liver PHx 4dpi", "GSM8403319", null, "source name:Liver|tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Partial Hepatectomy|geo loc name:missing|collection date:missing", "Liver PHx 4dpi", "A custom Cell Ranger index was generated from the Ensembl GRCz11 genome sequence and annotation filtered with \u2018mkgtf\u2019 command of Cell Ranger options: \u2018\u2014attribute = gene biotype:lincRNA \u2013attribute = gene biotype:antisense  as well as the pseudo mTagBFP2  H2B mGL  mCherry NTR sequences in these cells using the mkref command. This index was used to map reads and generate gene expression matrices using the \u2018count\u2019 command of the Cell Ranger software v.7.1.0 provided by 10x Genomics with the option \u201c\u2013include  introns\u201d set to False all other options were used as per default. Assembly: GRCz11 Supplementary files format and content: Count Matrix as h5ad file Supplementary files format and content: Metadata listing the cell annotations as csv file", "Liver", null, "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer\u2019s instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell 3\u2019 v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer\u2019s protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Partial Hepatectomy", "GSM8403319", "GSM8403319: Liver PHx 4dpi; Danio rerio; RNA Seq", "GSM8403319 r1", "GSM8403319", "1", "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer's instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell three prime v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer's protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 550", null, "SRP520597", null, "loader:fastq load.py", "ULB-CB-cr-zf-EES1-SEE-BBNTR-PHx-4dpi-GEM-04-04-24_S23_L003_I1_001.fastq.gz ULB-CB-cr-zf-EES1-SEE-BBNTR-PHx-4dpi-GEM-04-04-24_S23_L003_I2_001.fastq.gz ULB-CB-cr-zf-EES1-SEE-BBNTR-PHx-4dpi-GEM-04-04-24_S23_L003_R1_001.fastq.gz ULB-CB-cr-zf-EES1-SEE-BBNTR-PHx-4dpi-GEM-04-04-24_S23_L003_R2_001.fastq.gz", "fastq fastq fastq fastq", 18744044088.0, 111571691.0, "GSM8403319 r3", "0:10 1:10 2:28 3:120", "A:3967793098;C:2986585695;G:3048563710;T:3385544059;N:116358", 10, 10, 28, 120, 3967793098, 2986585695, 3048563710, 3385544059, 116358, "SRX25365556", "SRS22031296", "SRA1926595", "Regeneration and Stress Biology, IRIBHM, ULB", "Regeneration and Stress Biology, IRIBHM, ULB", 1, 0.9274, null, 0.07865, null, 0.82605, null, 0.61088, null, 120, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "Belgium", "2024-07-17", "Undetermined", "Larval", "Liver", "Liver and Biliary System"], [33217, "SRR29868297", "SRX25365556", "SRS22031296", "SRP520597", "PRJNA1136957", "Cholangiocytes contribute to hepatocyte regeneration post partial liver injury in larval zebrafish", "GSE272484", "Transcriptome Analysis", "Liver possesses robust regenerative ability  characterized by flexibility in the cellular source of regeneration based on the extent of the injury. post partial hepatectomy or minor injuries  hepatocytes  the primary liver cells  undergo self duplication to replenish the liver mass. In contrast  when the damage is extensive  or hepatocyte proliferation is impaired  cholangiocytes contribute to hepatocyte recovery. This current paradigm of regenerative flexibility in the liver has been established for animals with little or no growth. However  the regenerative mechanisms during periods of growth in young animals remain unexplored. Here  we establish two new partial liver injury protocols in the zebrafish model of rapid growth during late larval stage and observe emergence of de novo hepatocytes in the presence of spared hepatocytes. Using single cell RNA sequencing and lineage tracing  we identify cholangiocytes as the source of de novo hepatocytes. Our study offers a new perspective on the current paradigm of liver regenerating by proposing cholangiocyte to hepatocyte transdifferentiation as the default mechanism of hepatocyte recovery in late larval stage zebrafish. Overall design: We conducted single cell RNA Sequencing using the 10x platform of larval zebrafish liver. This was performed in the basal state Control1  Control2  post partial ablation MTZ 0dppa  MTZ 1dppa and MTZ 9dppa and post partial hepatectomy PHx 4dpi and PHx 11dpi. The two control samples were not treated with 4 OHT  and were also not injured. The control samples are from 13 dpf zebrafish raised under normal rearing condition. For all experiments Tgfabp10a:CellCousin line was used.", null, "pubmed:40480975", null, "Liver PHx 4dpi", "GSM8403319", null, "source name:Liver|tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Partial Hepatectomy|geo loc name:missing|collection date:missing", "Liver PHx 4dpi", "A custom Cell Ranger index was generated from the Ensembl GRCz11 genome sequence and annotation filtered with \u2018mkgtf\u2019 command of Cell Ranger options: \u2018\u2014attribute = gene biotype:lincRNA \u2013attribute = gene biotype:antisense  as well as the pseudo mTagBFP2  H2B mGL  mCherry NTR sequences in these cells using the mkref command. This index was used to map reads and generate gene expression matrices using the \u2018count\u2019 command of the Cell Ranger software v.7.1.0 provided by 10x Genomics with the option \u201c\u2013include  introns\u201d set to False all other options were used as per default. Assembly: GRCz11 Supplementary files format and content: Count Matrix as h5ad file Supplementary files format and content: Metadata listing the cell annotations as csv file", "Liver", null, "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer\u2019s instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell 3\u2019 v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer\u2019s protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Partial Hepatectomy", "GSM8403319", "GSM8403319: Liver PHx 4dpi; Danio rerio; RNA Seq", "GSM8403319 r1", "GSM8403319", "1", "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer's instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell three prime v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer's protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 550", null, "SRP520597", null, "loader:fastq load.py", "ULB-CB-cr-zf-EES1-SEE-BBNTR-PHx-4dpi-GEM-04-04-24_S23_L004_I1_001.fastq.gz ULB-CB-cr-zf-EES1-SEE-BBNTR-PHx-4dpi-GEM-04-04-24_S23_L004_I2_001.fastq.gz ULB-CB-cr-zf-EES1-SEE-BBNTR-PHx-4dpi-GEM-04-04-24_S23_L004_R1_001.fastq.gz ULB-CB-cr-zf-EES1-SEE-BBNTR-PHx-4dpi-GEM-04-04-24_S23_L004_R2_001.fastq.gz", "fastq fastq fastq fastq", 18250884624.0, 108636218.0, "GSM8403319 r4", "0:10 1:10 2:28 3:120", "A:3870590300;C:2903010183;G:2961077382;T:3301541312;N:126983", 10, 10, 28, 120, 3870590300, 2903010183, 2961077382, 3301541312, 126983, "SRX25365556", "SRS22031296", "SRA1926595", "Regeneration and Stress Biology, IRIBHM, ULB", "Regeneration and Stress Biology, IRIBHM, ULB", 1, 0.92712, null, 0.07894, null, 0.82509, null, 0.60912, null, 120, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "Belgium", "2024-07-17", "Undetermined", "Larval", "Liver", "Liver and Biliary System"], [33218, "SRR29868298", "SRX25365555", "SRS22031295", "SRP520597", "PRJNA1136957", "Cholangiocytes contribute to hepatocyte regeneration post partial liver injury in larval zebrafish", "GSE272484", "Transcriptome Analysis", "Liver possesses robust regenerative ability  characterized by flexibility in the cellular source of regeneration based on the extent of the injury. post partial hepatectomy or minor injuries  hepatocytes  the primary liver cells  undergo self duplication to replenish the liver mass. In contrast  when the damage is extensive  or hepatocyte proliferation is impaired  cholangiocytes contribute to hepatocyte recovery. This current paradigm of regenerative flexibility in the liver has been established for animals with little or no growth. However  the regenerative mechanisms during periods of growth in young animals remain unexplored. Here  we establish two new partial liver injury protocols in the zebrafish model of rapid growth during late larval stage and observe emergence of de novo hepatocytes in the presence of spared hepatocytes. Using single cell RNA sequencing and lineage tracing  we identify cholangiocytes as the source of de novo hepatocytes. Our study offers a new perspective on the current paradigm of liver regenerating by proposing cholangiocyte to hepatocyte transdifferentiation as the default mechanism of hepatocyte recovery in late larval stage zebrafish. Overall design: We conducted single cell RNA Sequencing using the 10x platform of larval zebrafish liver. This was performed in the basal state Control1  Control2  post partial ablation MTZ 0dppa  MTZ 1dppa and MTZ 9dppa and post partial hepatectomy PHx 4dpi and PHx 11dpi. The two control samples were not treated with 4 OHT  and were also not injured. The control samples are from 13 dpf zebrafish raised under normal rearing condition. For all experiments Tgfabp10a:CellCousin line was used.", null, "pubmed:40480975", null, "Liver MTZ 9dppa", "GSM8403318", null, "source name:Liver|tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Partial Ablation|geo loc name:missing|collection date:missing", "Liver MTZ 9dppa", "A custom Cell Ranger index was generated from the Ensembl GRCz11 genome sequence and annotation filtered with \u2018mkgtf\u2019 command of Cell Ranger options: \u2018\u2014attribute = gene biotype:lincRNA \u2013attribute = gene biotype:antisense  as well as the pseudo mTagBFP2  H2B mGL  mCherry NTR sequences in these cells using the mkref command. This index was used to map reads and generate gene expression matrices using the \u2018count\u2019 command of the Cell Ranger software v.7.1.0 provided by 10x Genomics with the option \u201c\u2013include  introns\u201d set to False all other options were used as per default. Assembly: GRCz11 Supplementary files format and content: Count Matrix as h5ad file Supplementary files format and content: Metadata listing the cell annotations as csv file", "Liver", null, "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer\u2019s instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell 3\u2019 v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer\u2019s protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Partial Ablation", "GSM8403318", "GSM8403318: Liver MTZ 9dppa; Danio rerio; RNA Seq", "GSM8403318 r1", "GSM8403318", "1", "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer's instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell three prime v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer's protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 550", null, "SRP520597", null, "loader:fastq load.py", "ULB-CB-cr-zf-EES3-SEE-MTZ-9dppa-GEM-26-04-24_S26_L001_R2_001.fastq.gz ULB-CB-cr-zf-EES3-SEE-MTZ-9dppa-GEM-26-04-24_S26_L001_R1_001.fastq.gz ULB-CB-cr-zf-EES3-SEE-MTZ-9dppa-GEM-26-04-24_S26_L001_I2_001.fastq.gz ULB-CB-cr-zf-EES3-SEE-MTZ-9dppa-GEM-26-04-24_S26_L001_I1_001.fastq.gz", "fastq fastq fastq fastq", 17110767912.0, 101849809.0, "GSM8403318 r1", "0:10 1:10 2:28 3:120", "A:3788205667;C:2699657929;G:2699620981;T:3034435232;N:57271", 10, 10, 28, 120, 3788205667, 2699657929, 2699620981, 3034435232, 57271, "SRX25365555", "SRS22031295", "SRA1926595", "Regeneration and Stress Biology, IRIBHM, ULB", "Regeneration and Stress Biology, IRIBHM, ULB", 1, 0.91437, null, 0.05435, null, 0.86983, null, 0.62046, null, 120, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "Belgium", "2024-07-17", "Undetermined", "Larval", "Liver", "Liver and Biliary System"], [33219, "SRR29868299", "SRX25365555", "SRS22031295", "SRP520597", "PRJNA1136957", "Cholangiocytes contribute to hepatocyte regeneration post partial liver injury in larval zebrafish", "GSE272484", "Transcriptome Analysis", "Liver possesses robust regenerative ability  characterized by flexibility in the cellular source of regeneration based on the extent of the injury. post partial hepatectomy or minor injuries  hepatocytes  the primary liver cells  undergo self duplication to replenish the liver mass. In contrast  when the damage is extensive  or hepatocyte proliferation is impaired  cholangiocytes contribute to hepatocyte recovery. This current paradigm of regenerative flexibility in the liver has been established for animals with little or no growth. However  the regenerative mechanisms during periods of growth in young animals remain unexplored. Here  we establish two new partial liver injury protocols in the zebrafish model of rapid growth during late larval stage and observe emergence of de novo hepatocytes in the presence of spared hepatocytes. Using single cell RNA sequencing and lineage tracing  we identify cholangiocytes as the source of de novo hepatocytes. Our study offers a new perspective on the current paradigm of liver regenerating by proposing cholangiocyte to hepatocyte transdifferentiation as the default mechanism of hepatocyte recovery in late larval stage zebrafish. Overall design: We conducted single cell RNA Sequencing using the 10x platform of larval zebrafish liver. This was performed in the basal state Control1  Control2  post partial ablation MTZ 0dppa  MTZ 1dppa and MTZ 9dppa and post partial hepatectomy PHx 4dpi and PHx 11dpi. The two control samples were not treated with 4 OHT  and were also not injured. The control samples are from 13 dpf zebrafish raised under normal rearing condition. For all experiments Tgfabp10a:CellCousin line was used.", null, "pubmed:40480975", null, "Liver MTZ 9dppa", "GSM8403318", null, "source name:Liver|tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Partial Ablation|geo loc name:missing|collection date:missing", "Liver MTZ 9dppa", "A custom Cell Ranger index was generated from the Ensembl GRCz11 genome sequence and annotation filtered with \u2018mkgtf\u2019 command of Cell Ranger options: \u2018\u2014attribute = gene biotype:lincRNA \u2013attribute = gene biotype:antisense  as well as the pseudo mTagBFP2  H2B mGL  mCherry NTR sequences in these cells using the mkref command. This index was used to map reads and generate gene expression matrices using the \u2018count\u2019 command of the Cell Ranger software v.7.1.0 provided by 10x Genomics with the option \u201c\u2013include  introns\u201d set to False all other options were used as per default. Assembly: GRCz11 Supplementary files format and content: Count Matrix as h5ad file Supplementary files format and content: Metadata listing the cell annotations as csv file", "Liver", null, "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer\u2019s instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell 3\u2019 v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer\u2019s protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Partial Ablation", "GSM8403318", "GSM8403318: Liver MTZ 9dppa; Danio rerio; RNA Seq", "GSM8403318 r1", "GSM8403318", "1", "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer's instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell three prime v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer's protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 550", null, "SRP520597", null, "loader:fastq load.py", "ULB-CB-cr-zf-EES3-SEE-MTZ-9dppa-GEM-26-04-24_S26_L003_R2_001.fastq.gz ULB-CB-cr-zf-EES3-SEE-MTZ-9dppa-GEM-26-04-24_S26_L003_R1_001.fastq.gz ULB-CB-cr-zf-EES3-SEE-MTZ-9dppa-GEM-26-04-24_S26_L003_I2_001.fastq.gz ULB-CB-cr-zf-EES3-SEE-MTZ-9dppa-GEM-26-04-24_S26_L003_I1_001.fastq.gz", "fastq fastq fastq fastq", 17981827752.0, 107034689.0, "GSM8403318 r3", "0:10 1:10 2:28 3:120", "A:3977642248;C:2840653787;G:2844602651;T:3181150070;N:113924", 10, 10, 28, 120, 3977642248, 2840653787, 2844602651, 3181150070, 113924, "SRX25365555", "SRS22031295", "SRA1926595", "Regeneration and Stress Biology, IRIBHM, ULB", "Regeneration and Stress Biology, IRIBHM, ULB", 1, 0.91394, null, 0.05332, null, 0.87, null, 0.61803, null, 120, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "Belgium", "2024-07-17", "Undetermined", "Larval", "Liver", "Liver and Biliary System"], [33220, "SRR29868300", "SRX25365555", "SRS22031295", "SRP520597", "PRJNA1136957", "Cholangiocytes contribute to hepatocyte regeneration post partial liver injury in larval zebrafish", "GSE272484", "Transcriptome Analysis", "Liver possesses robust regenerative ability  characterized by flexibility in the cellular source of regeneration based on the extent of the injury. post partial hepatectomy or minor injuries  hepatocytes  the primary liver cells  undergo self duplication to replenish the liver mass. In contrast  when the damage is extensive  or hepatocyte proliferation is impaired  cholangiocytes contribute to hepatocyte recovery. This current paradigm of regenerative flexibility in the liver has been established for animals with little or no growth. However  the regenerative mechanisms during periods of growth in young animals remain unexplored. Here  we establish two new partial liver injury protocols in the zebrafish model of rapid growth during late larval stage and observe emergence of de novo hepatocytes in the presence of spared hepatocytes. Using single cell RNA sequencing and lineage tracing  we identify cholangiocytes as the source of de novo hepatocytes. Our study offers a new perspective on the current paradigm of liver regenerating by proposing cholangiocyte to hepatocyte transdifferentiation as the default mechanism of hepatocyte recovery in late larval stage zebrafish. Overall design: We conducted single cell RNA Sequencing using the 10x platform of larval zebrafish liver. This was performed in the basal state Control1  Control2  post partial ablation MTZ 0dppa  MTZ 1dppa and MTZ 9dppa and post partial hepatectomy PHx 4dpi and PHx 11dpi. The two control samples were not treated with 4 OHT  and were also not injured. The control samples are from 13 dpf zebrafish raised under normal rearing condition. For all experiments Tgfabp10a:CellCousin line was used.", null, "pubmed:40480975", null, "Liver MTZ 9dppa", "GSM8403318", null, "source name:Liver|tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Partial Ablation|geo loc name:missing|collection date:missing", "Liver MTZ 9dppa", "A custom Cell Ranger index was generated from the Ensembl GRCz11 genome sequence and annotation filtered with \u2018mkgtf\u2019 command of Cell Ranger options: \u2018\u2014attribute = gene biotype:lincRNA \u2013attribute = gene biotype:antisense  as well as the pseudo mTagBFP2  H2B mGL  mCherry NTR sequences in these cells using the mkref command. This index was used to map reads and generate gene expression matrices using the \u2018count\u2019 command of the Cell Ranger software v.7.1.0 provided by 10x Genomics with the option \u201c\u2013include  introns\u201d set to False all other options were used as per default. Assembly: GRCz11 Supplementary files format and content: Count Matrix as h5ad file Supplementary files format and content: Metadata listing the cell annotations as csv file", "Liver", null, "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer\u2019s instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell 3\u2019 v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer\u2019s protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Partial Ablation", "GSM8403318", "GSM8403318: Liver MTZ 9dppa; Danio rerio; RNA Seq", "GSM8403318 r1", "GSM8403318", "1", "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer's instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell three prime v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer's protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 550", null, "SRP520597", null, "loader:fastq load.py", "ULB-CB-cr-zf-EES3-SEE-MTZ-9dppa-GEM-26-04-24_S26_L004_R2_001.fastq.gz ULB-CB-cr-zf-EES3-SEE-MTZ-9dppa-GEM-26-04-24_S26_L004_R1_001.fastq.gz ULB-CB-cr-zf-EES3-SEE-MTZ-9dppa-GEM-26-04-24_S26_L004_I2_001.fastq.gz ULB-CB-cr-zf-EES3-SEE-MTZ-9dppa-GEM-26-04-24_S26_L004_I1_001.fastq.gz", "fastq fastq fastq fastq", 17550170232.0, 104465299.0, "GSM8403318 r4", "0:10 1:10 2:28 3:120", "A:3887199949;C:2768808747;G:2769733142;T:3109971563;N:122479", 10, 10, 28, 120, 3887199949, 2768808747, 2769733142, 3109971563, 122479, "SRX25365555", "SRS22031295", "SRA1926595", "Regeneration and Stress Biology, IRIBHM, ULB", "Regeneration and Stress Biology, IRIBHM, ULB", 1, 0.91342, null, 0.0541, null, 0.87046, null, 0.61388, null, 120, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "Belgium", "2024-07-17", "Undetermined", "Larval", "Liver", "Liver and Biliary System"], [33221, "SRR29868306", "SRX25365555", "SRS22031295", "SRP520597", "PRJNA1136957", "Cholangiocytes contribute to hepatocyte regeneration post partial liver injury in larval zebrafish", "GSE272484", "Transcriptome Analysis", "Liver possesses robust regenerative ability  characterized by flexibility in the cellular source of regeneration based on the extent of the injury. post partial hepatectomy or minor injuries  hepatocytes  the primary liver cells  undergo self duplication to replenish the liver mass. In contrast  when the damage is extensive  or hepatocyte proliferation is impaired  cholangiocytes contribute to hepatocyte recovery. This current paradigm of regenerative flexibility in the liver has been established for animals with little or no growth. However  the regenerative mechanisms during periods of growth in young animals remain unexplored. Here  we establish two new partial liver injury protocols in the zebrafish model of rapid growth during late larval stage and observe emergence of de novo hepatocytes in the presence of spared hepatocytes. Using single cell RNA sequencing and lineage tracing  we identify cholangiocytes as the source of de novo hepatocytes. Our study offers a new perspective on the current paradigm of liver regenerating by proposing cholangiocyte to hepatocyte transdifferentiation as the default mechanism of hepatocyte recovery in late larval stage zebrafish. Overall design: We conducted single cell RNA Sequencing using the 10x platform of larval zebrafish liver. This was performed in the basal state Control1  Control2  post partial ablation MTZ 0dppa  MTZ 1dppa and MTZ 9dppa and post partial hepatectomy PHx 4dpi and PHx 11dpi. The two control samples were not treated with 4 OHT  and were also not injured. The control samples are from 13 dpf zebrafish raised under normal rearing condition. For all experiments Tgfabp10a:CellCousin line was used.", null, "pubmed:40480975", null, "Liver MTZ 9dppa", "GSM8403318", null, "source name:Liver|tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Partial Ablation|geo loc name:missing|collection date:missing", "Liver MTZ 9dppa", "A custom Cell Ranger index was generated from the Ensembl GRCz11 genome sequence and annotation filtered with \u2018mkgtf\u2019 command of Cell Ranger options: \u2018\u2014attribute = gene biotype:lincRNA \u2013attribute = gene biotype:antisense  as well as the pseudo mTagBFP2  H2B mGL  mCherry NTR sequences in these cells using the mkref command. This index was used to map reads and generate gene expression matrices using the \u2018count\u2019 command of the Cell Ranger software v.7.1.0 provided by 10x Genomics with the option \u201c\u2013include  introns\u201d set to False all other options were used as per default. Assembly: GRCz11 Supplementary files format and content: Count Matrix as h5ad file Supplementary files format and content: Metadata listing the cell annotations as csv file", "Liver", null, "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer\u2019s instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell 3\u2019 v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer\u2019s protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Partial Ablation", "GSM8403318", "GSM8403318: Liver MTZ 9dppa; Danio rerio; RNA Seq", "GSM8403318 r1", "GSM8403318", "1", "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer's instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell three prime v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer's protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 550", null, "SRP520597", null, "loader:fastq load.py", "ULB-CB-cr-zf-EES3-SEE-MTZ-9dppa-GEM-26-04-24_S26_L002_R2_001.fastq.gz ULB-CB-cr-zf-EES3-SEE-MTZ-9dppa-GEM-26-04-24_S26_L002_R1_001.fastq.gz ULB-CB-cr-zf-EES3-SEE-MTZ-9dppa-GEM-26-04-24_S26_L002_I2_001.fastq.gz ULB-CB-cr-zf-EES3-SEE-MTZ-9dppa-GEM-26-04-24_S26_L002_I1_001.fastq.gz", "fastq fastq fastq fastq", 17719057944.0, 105470583.0, "GSM8403318 r2", "0:10 1:10 2:28 3:120", "A:3923720947;C:2796640453;G:2800418238;T:3135616927;N:73395", 10, 10, 28, 120, 3923720947, 2796640453, 2800418238, 3135616927, 73395, "SRX25365555", "SRS22031295", "SRA1926595", "Regeneration and Stress Biology, IRIBHM, ULB", "Regeneration and Stress Biology, IRIBHM, ULB", 1, 0.91325, null, 0.05422, null, 0.86949, null, 0.6266, null, 120, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "Belgium", "2024-07-17", "Undetermined", "Larval", "Liver", "Liver and Biliary System"], [33222, "SRR29868301", "SRX25365554", "SRS22031294", "SRP520597", "PRJNA1136957", "Cholangiocytes contribute to hepatocyte regeneration post partial liver injury in larval zebrafish", "GSE272484", "Transcriptome Analysis", "Liver possesses robust regenerative ability  characterized by flexibility in the cellular source of regeneration based on the extent of the injury. post partial hepatectomy or minor injuries  hepatocytes  the primary liver cells  undergo self duplication to replenish the liver mass. In contrast  when the damage is extensive  or hepatocyte proliferation is impaired  cholangiocytes contribute to hepatocyte recovery. This current paradigm of regenerative flexibility in the liver has been established for animals with little or no growth. However  the regenerative mechanisms during periods of growth in young animals remain unexplored. Here  we establish two new partial liver injury protocols in the zebrafish model of rapid growth during late larval stage and observe emergence of de novo hepatocytes in the presence of spared hepatocytes. Using single cell RNA sequencing and lineage tracing  we identify cholangiocytes as the source of de novo hepatocytes. Our study offers a new perspective on the current paradigm of liver regenerating by proposing cholangiocyte to hepatocyte transdifferentiation as the default mechanism of hepatocyte recovery in late larval stage zebrafish. Overall design: We conducted single cell RNA Sequencing using the 10x platform of larval zebrafish liver. This was performed in the basal state Control1  Control2  post partial ablation MTZ 0dppa  MTZ 1dppa and MTZ 9dppa and post partial hepatectomy PHx 4dpi and PHx 11dpi. The two control samples were not treated with 4 OHT  and were also not injured. The control samples are from 13 dpf zebrafish raised under normal rearing condition. For all experiments Tgfabp10a:CellCousin line was used.", null, "pubmed:40480975", null, "Liver MTZ 1dppa", "GSM8403317", null, "source name:Liver|tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Partial Ablation|geo loc name:missing|collection date:missing", "Liver MTZ 1dppa", "A custom Cell Ranger index was generated from the Ensembl GRCz11 genome sequence and annotation filtered with \u2018mkgtf\u2019 command of Cell Ranger options: \u2018\u2014attribute = gene biotype:lincRNA \u2013attribute = gene biotype:antisense  as well as the pseudo mTagBFP2  H2B mGL  mCherry NTR sequences in these cells using the mkref command. This index was used to map reads and generate gene expression matrices using the \u2018count\u2019 command of the Cell Ranger software v.7.1.0 provided by 10x Genomics with the option \u201c\u2013include  introns\u201d set to False all other options were used as per default. Assembly: GRCz11 Supplementary files format and content: Count Matrix as h5ad file Supplementary files format and content: Metadata listing the cell annotations as csv file", "Liver", null, "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer\u2019s instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell 3\u2019 v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer\u2019s protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Partial Ablation", "GSM8403317", "GSM8403317: Liver MTZ 1dppa; Danio rerio; RNA Seq", "GSM8403317 r1", "GSM8403317", "1", "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer's instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell three prime v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer's protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 550", null, "SRP520597", null, "loader:fastq load.py", "ULB-CB-cr-zf-EES2-reload-EES3-1dppa-GEM-240122_S18_L001_I1_001.fastq.gz ULB-CB-cr-zf-EES2-reload-EES3-1dppa-GEM-240122_S18_L001_I2_001.fastq.gz ULB-CB-cr-zf-EES2-reload-EES3-1dppa-GEM-240122_S18_L001_R1_001.fastq.gz ULB-CB-cr-zf-EES2-reload-EES3-1dppa-GEM-240122_S18_L001_R2_001.fastq.gz", "fastq fastq fastq fastq", 21323163288.0, 126923591.0, "GSM8403317 r1", "0:10 1:10 2:28 3:120", "A:4482829539;C:3366994739;G:3484245391;T:3896538980;N:222271", 10, 10, 28, 120, 4482829539, 3366994739, 3484245391, 3896538980, 222271, "SRX25365554", "SRS22031294", "SRA1926595", "Regeneration and Stress Biology, IRIBHM, ULB", "Regeneration and Stress Biology, IRIBHM, ULB", 1, 0.93349, null, 0.07482, null, 0.81925, null, 0.57693, null, 120, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "Belgium", "2024-07-17", "Undetermined", "Larval", "Liver", "Liver and Biliary System"], [33223, "SRR29868302", "SRX25365554", "SRS22031294", "SRP520597", "PRJNA1136957", "Cholangiocytes contribute to hepatocyte regeneration post partial liver injury in larval zebrafish", "GSE272484", "Transcriptome Analysis", "Liver possesses robust regenerative ability  characterized by flexibility in the cellular source of regeneration based on the extent of the injury. post partial hepatectomy or minor injuries  hepatocytes  the primary liver cells  undergo self duplication to replenish the liver mass. In contrast  when the damage is extensive  or hepatocyte proliferation is impaired  cholangiocytes contribute to hepatocyte recovery. This current paradigm of regenerative flexibility in the liver has been established for animals with little or no growth. However  the regenerative mechanisms during periods of growth in young animals remain unexplored. Here  we establish two new partial liver injury protocols in the zebrafish model of rapid growth during late larval stage and observe emergence of de novo hepatocytes in the presence of spared hepatocytes. Using single cell RNA sequencing and lineage tracing  we identify cholangiocytes as the source of de novo hepatocytes. Our study offers a new perspective on the current paradigm of liver regenerating by proposing cholangiocyte to hepatocyte transdifferentiation as the default mechanism of hepatocyte recovery in late larval stage zebrafish. Overall design: We conducted single cell RNA Sequencing using the 10x platform of larval zebrafish liver. This was performed in the basal state Control1  Control2  post partial ablation MTZ 0dppa  MTZ 1dppa and MTZ 9dppa and post partial hepatectomy PHx 4dpi and PHx 11dpi. The two control samples were not treated with 4 OHT  and were also not injured. The control samples are from 13 dpf zebrafish raised under normal rearing condition. For all experiments Tgfabp10a:CellCousin line was used.", null, "pubmed:40480975", null, "Liver MTZ 1dppa", "GSM8403317", null, "source name:Liver|tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Partial Ablation|geo loc name:missing|collection date:missing", "Liver MTZ 1dppa", "A custom Cell Ranger index was generated from the Ensembl GRCz11 genome sequence and annotation filtered with \u2018mkgtf\u2019 command of Cell Ranger options: \u2018\u2014attribute = gene biotype:lincRNA \u2013attribute = gene biotype:antisense  as well as the pseudo mTagBFP2  H2B mGL  mCherry NTR sequences in these cells using the mkref command. This index was used to map reads and generate gene expression matrices using the \u2018count\u2019 command of the Cell Ranger software v.7.1.0 provided by 10x Genomics with the option \u201c\u2013include  introns\u201d set to False all other options were used as per default. Assembly: GRCz11 Supplementary files format and content: Count Matrix as h5ad file Supplementary files format and content: Metadata listing the cell annotations as csv file", "Liver", null, "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer\u2019s instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell 3\u2019 v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer\u2019s protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Partial Ablation", "GSM8403317", "GSM8403317: Liver MTZ 1dppa; Danio rerio; RNA Seq", "GSM8403317 r1", "GSM8403317", "1", "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer's instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell three prime v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer's protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 550", null, "SRP520597", null, "loader:fastq load.py", "ULB-CB-cr-zf-EES3-1dppa-GEM-240122_S35_L001_I1_001.fastq.gz ULB-CB-cr-zf-EES3-1dppa-GEM-240122_S35_L001_I2_001.fastq.gz ULB-CB-cr-zf-EES3-1dppa-GEM-240122_S35_L001_R2_001.fastq.gz ULB-CB-cr-zf-EES3-1dppa-GEM-240122_S35_L001_R1_001.fastq.gz", "fastq fastq fastq fastq", 7887231744.0, 46947808.0, "GSM8403317 r5", "0:10 1:10 2:28 3:120", "A:1641421944;C:1251181706;G:1291844073;T:1449242410;N:46827", 10, 10, 28, 120, 1641421944, 1251181706, 1291844073, 1449242410, 46827, "SRX25365554", "SRS22031294", "SRA1926595", "Regeneration and Stress Biology, IRIBHM, ULB", "Regeneration and Stress Biology, IRIBHM, ULB", 1, 0.93251, null, 0.07497, null, 0.81763, null, 0.58245, null, 120, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "Belgium", "2024-07-17", "Undetermined", "Larval", "Liver", "Liver and Biliary System"], [33224, "SRR29868303", "SRX25365554", "SRS22031294", "SRP520597", "PRJNA1136957", "Cholangiocytes contribute to hepatocyte regeneration post partial liver injury in larval zebrafish", "GSE272484", "Transcriptome Analysis", "Liver possesses robust regenerative ability  characterized by flexibility in the cellular source of regeneration based on the extent of the injury. post partial hepatectomy or minor injuries  hepatocytes  the primary liver cells  undergo self duplication to replenish the liver mass. In contrast  when the damage is extensive  or hepatocyte proliferation is impaired  cholangiocytes contribute to hepatocyte recovery. This current paradigm of regenerative flexibility in the liver has been established for animals with little or no growth. However  the regenerative mechanisms during periods of growth in young animals remain unexplored. Here  we establish two new partial liver injury protocols in the zebrafish model of rapid growth during late larval stage and observe emergence of de novo hepatocytes in the presence of spared hepatocytes. Using single cell RNA sequencing and lineage tracing  we identify cholangiocytes as the source of de novo hepatocytes. Our study offers a new perspective on the current paradigm of liver regenerating by proposing cholangiocyte to hepatocyte transdifferentiation as the default mechanism of hepatocyte recovery in late larval stage zebrafish. Overall design: We conducted single cell RNA Sequencing using the 10x platform of larval zebrafish liver. This was performed in the basal state Control1  Control2  post partial ablation MTZ 0dppa  MTZ 1dppa and MTZ 9dppa and post partial hepatectomy PHx 4dpi and PHx 11dpi. The two control samples were not treated with 4 OHT  and were also not injured. The control samples are from 13 dpf zebrafish raised under normal rearing condition. For all experiments Tgfabp10a:CellCousin line was used.", null, "pubmed:40480975", null, "Liver MTZ 1dppa", "GSM8403317", null, "source name:Liver|tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Partial Ablation|geo loc name:missing|collection date:missing", "Liver MTZ 1dppa", "A custom Cell Ranger index was generated from the Ensembl GRCz11 genome sequence and annotation filtered with \u2018mkgtf\u2019 command of Cell Ranger options: \u2018\u2014attribute = gene biotype:lincRNA \u2013attribute = gene biotype:antisense  as well as the pseudo mTagBFP2  H2B mGL  mCherry NTR sequences in these cells using the mkref command. This index was used to map reads and generate gene expression matrices using the \u2018count\u2019 command of the Cell Ranger software v.7.1.0 provided by 10x Genomics with the option \u201c\u2013include  introns\u201d set to False all other options were used as per default. Assembly: GRCz11 Supplementary files format and content: Count Matrix as h5ad file Supplementary files format and content: Metadata listing the cell annotations as csv file", "Liver", null, "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer\u2019s instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell 3\u2019 v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer\u2019s protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Partial Ablation", "GSM8403317", "GSM8403317: Liver MTZ 1dppa; Danio rerio; RNA Seq", "GSM8403317 r1", "GSM8403317", "1", "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer's instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell three prime v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer's protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 550", null, "SRP520597", null, "loader:fastq load.py", "ULB-CB-cr-zf-EES3-1dppa-GEM-240122_S35_L002_I1_001.fastq.gz ULB-CB-cr-zf-EES3-1dppa-GEM-240122_S35_L002_I2_001.fastq.gz ULB-CB-cr-zf-EES3-1dppa-GEM-240122_S35_L002_R1_001.fastq.gz ULB-CB-cr-zf-EES3-1dppa-GEM-240122_S35_L002_R2_001.fastq.gz", "fastq fastq fastq fastq", 8028118896.0, 47786422.0, "GSM8403317 r6", "0:10 1:10 2:28 3:120", "A:1672066662;C:1273372069;G:1312792441;T:1476102756;N:36712", 10, 10, 28, 120, 1672066662, 1273372069, 1312792441, 1476102756, 36712, "SRX25365554", "SRS22031294", "SRA1926595", "Regeneration and Stress Biology, IRIBHM, ULB", "Regeneration and Stress Biology, IRIBHM, ULB", 1, 0.93266, null, 0.07544, null, 0.81675, null, 0.57597, null, 120, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "Belgium", "2024-07-17", "Undetermined", "Larval", "Liver", "Liver and Biliary System"], [33225, "SRR29868304", "SRX25365554", "SRS22031294", "SRP520597", "PRJNA1136957", "Cholangiocytes contribute to hepatocyte regeneration post partial liver injury in larval zebrafish", "GSE272484", "Transcriptome Analysis", "Liver possesses robust regenerative ability  characterized by flexibility in the cellular source of regeneration based on the extent of the injury. post partial hepatectomy or minor injuries  hepatocytes  the primary liver cells  undergo self duplication to replenish the liver mass. In contrast  when the damage is extensive  or hepatocyte proliferation is impaired  cholangiocytes contribute to hepatocyte recovery. This current paradigm of regenerative flexibility in the liver has been established for animals with little or no growth. However  the regenerative mechanisms during periods of growth in young animals remain unexplored. Here  we establish two new partial liver injury protocols in the zebrafish model of rapid growth during late larval stage and observe emergence of de novo hepatocytes in the presence of spared hepatocytes. Using single cell RNA sequencing and lineage tracing  we identify cholangiocytes as the source of de novo hepatocytes. Our study offers a new perspective on the current paradigm of liver regenerating by proposing cholangiocyte to hepatocyte transdifferentiation as the default mechanism of hepatocyte recovery in late larval stage zebrafish. Overall design: We conducted single cell RNA Sequencing using the 10x platform of larval zebrafish liver. This was performed in the basal state Control1  Control2  post partial ablation MTZ 0dppa  MTZ 1dppa and MTZ 9dppa and post partial hepatectomy PHx 4dpi and PHx 11dpi. The two control samples were not treated with 4 OHT  and were also not injured. The control samples are from 13 dpf zebrafish raised under normal rearing condition. For all experiments Tgfabp10a:CellCousin line was used.", null, "pubmed:40480975", null, "Liver MTZ 1dppa", "GSM8403317", null, "source name:Liver|tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Partial Ablation|geo loc name:missing|collection date:missing", "Liver MTZ 1dppa", "A custom Cell Ranger index was generated from the Ensembl GRCz11 genome sequence and annotation filtered with \u2018mkgtf\u2019 command of Cell Ranger options: \u2018\u2014attribute = gene biotype:lincRNA \u2013attribute = gene biotype:antisense  as well as the pseudo mTagBFP2  H2B mGL  mCherry NTR sequences in these cells using the mkref command. This index was used to map reads and generate gene expression matrices using the \u2018count\u2019 command of the Cell Ranger software v.7.1.0 provided by 10x Genomics with the option \u201c\u2013include  introns\u201d set to False all other options were used as per default. Assembly: GRCz11 Supplementary files format and content: Count Matrix as h5ad file Supplementary files format and content: Metadata listing the cell annotations as csv file", "Liver", null, "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer\u2019s instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell 3\u2019 v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer\u2019s protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Partial Ablation", "GSM8403317", "GSM8403317: Liver MTZ 1dppa; Danio rerio; RNA Seq", "GSM8403317 r1", "GSM8403317", "1", "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer's instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell three prime v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer's protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 550", null, "SRP520597", null, "loader:fastq load.py", "ULB-CB-cr-zf-EES3-1dppa-GEM-240122_S35_L003_I1_001.fastq.gz ULB-CB-cr-zf-EES3-1dppa-GEM-240122_S35_L003_I2_001.fastq.gz ULB-CB-cr-zf-EES3-1dppa-GEM-240122_S35_L003_R1_001.fastq.gz ULB-CB-cr-zf-EES3-1dppa-GEM-240122_S35_L003_R2_001.fastq.gz", "fastq fastq fastq fastq", 8223531120.0, 48949590.0, "GSM8403317 r7", "0:10 1:10 2:28 3:120", "A:1713836781;C:1304685662;G:1345155931;T:1510228105;N:44321", 10, 10, 28, 120, 1713836781, 1304685662, 1345155931, 1510228105, 44321, "SRX25365554", "SRS22031294", "SRA1926595", "Regeneration and Stress Biology, IRIBHM, ULB", "Regeneration and Stress Biology, IRIBHM, ULB", 1, 0.93273, null, 0.07513, null, 0.81546, null, 0.58099, null, 120, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "Belgium", "2024-07-17", "Undetermined", "Larval", "Liver", "Liver and Biliary System"], [33226, "SRR29868305", "SRX25365554", "SRS22031294", "SRP520597", "PRJNA1136957", "Cholangiocytes contribute to hepatocyte regeneration post partial liver injury in larval zebrafish", "GSE272484", "Transcriptome Analysis", "Liver possesses robust regenerative ability  characterized by flexibility in the cellular source of regeneration based on the extent of the injury. post partial hepatectomy or minor injuries  hepatocytes  the primary liver cells  undergo self duplication to replenish the liver mass. In contrast  when the damage is extensive  or hepatocyte proliferation is impaired  cholangiocytes contribute to hepatocyte recovery. This current paradigm of regenerative flexibility in the liver has been established for animals with little or no growth. However  the regenerative mechanisms during periods of growth in young animals remain unexplored. Here  we establish two new partial liver injury protocols in the zebrafish model of rapid growth during late larval stage and observe emergence of de novo hepatocytes in the presence of spared hepatocytes. Using single cell RNA sequencing and lineage tracing  we identify cholangiocytes as the source of de novo hepatocytes. Our study offers a new perspective on the current paradigm of liver regenerating by proposing cholangiocyte to hepatocyte transdifferentiation as the default mechanism of hepatocyte recovery in late larval stage zebrafish. Overall design: We conducted single cell RNA Sequencing using the 10x platform of larval zebrafish liver. This was performed in the basal state Control1  Control2  post partial ablation MTZ 0dppa  MTZ 1dppa and MTZ 9dppa and post partial hepatectomy PHx 4dpi and PHx 11dpi. The two control samples were not treated with 4 OHT  and were also not injured. The control samples are from 13 dpf zebrafish raised under normal rearing condition. For all experiments Tgfabp10a:CellCousin line was used.", null, "pubmed:40480975", null, "Liver MTZ 1dppa", "GSM8403317", null, "source name:Liver|tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Partial Ablation|geo loc name:missing|collection date:missing", "Liver MTZ 1dppa", "A custom Cell Ranger index was generated from the Ensembl GRCz11 genome sequence and annotation filtered with \u2018mkgtf\u2019 command of Cell Ranger options: \u2018\u2014attribute = gene biotype:lincRNA \u2013attribute = gene biotype:antisense  as well as the pseudo mTagBFP2  H2B mGL  mCherry NTR sequences in these cells using the mkref command. This index was used to map reads and generate gene expression matrices using the \u2018count\u2019 command of the Cell Ranger software v.7.1.0 provided by 10x Genomics with the option \u201c\u2013include  introns\u201d set to False all other options were used as per default. Assembly: GRCz11 Supplementary files format and content: Count Matrix as h5ad file Supplementary files format and content: Metadata listing the cell annotations as csv file", "Liver", null, "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer\u2019s instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell 3\u2019 v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer\u2019s protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Partial Ablation", "GSM8403317", "GSM8403317: Liver MTZ 1dppa; Danio rerio; RNA Seq", "GSM8403317 r1", "GSM8403317", "1", "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer's instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell three prime v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer's protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 550", null, "SRP520597", null, "loader:fastq load.py", "ULB-CB-cr-zf-EES3-1dppa-GEM-240122_S35_L004_R2_001.fastq.gz ULB-CB-cr-zf-EES3-1dppa-GEM-240122_S35_L004_R1_001.fastq.gz ULB-CB-cr-zf-EES3-1dppa-GEM-240122_S35_L004_I2_001.fastq.gz ULB-CB-cr-zf-EES3-1dppa-GEM-240122_S35_L004_I1_001.fastq.gz", "fastq fastq fastq fastq", 7715042160.0, 45922870.0, "GSM8403317 r8", "0:10 1:10 2:28 3:120", "A:1604997878;C:1222312105;G:1258545155;T:1424847802;N:41460", 10, 10, 28, 120, 1604997878, 1222312105, 1258545155, 1424847802, 41460, "SRX25365554", "SRS22031294", "SRA1926595", "Regeneration and Stress Biology, IRIBHM, ULB", "Regeneration and Stress Biology, IRIBHM, ULB", 1, 0.93359, null, 0.07524, null, 0.81479, null, 0.57882, null, 120, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "Belgium", "2024-07-17", "Undetermined", "Larval", "Liver", "Liver and Biliary System"], [33227, "SRR29868320", "SRX25365554", "SRS22031294", "SRP520597", "PRJNA1136957", "Cholangiocytes contribute to hepatocyte regeneration post partial liver injury in larval zebrafish", "GSE272484", "Transcriptome Analysis", "Liver possesses robust regenerative ability  characterized by flexibility in the cellular source of regeneration based on the extent of the injury. post partial hepatectomy or minor injuries  hepatocytes  the primary liver cells  undergo self duplication to replenish the liver mass. In contrast  when the damage is extensive  or hepatocyte proliferation is impaired  cholangiocytes contribute to hepatocyte recovery. This current paradigm of regenerative flexibility in the liver has been established for animals with little or no growth. However  the regenerative mechanisms during periods of growth in young animals remain unexplored. Here  we establish two new partial liver injury protocols in the zebrafish model of rapid growth during late larval stage and observe emergence of de novo hepatocytes in the presence of spared hepatocytes. Using single cell RNA sequencing and lineage tracing  we identify cholangiocytes as the source of de novo hepatocytes. Our study offers a new perspective on the current paradigm of liver regenerating by proposing cholangiocyte to hepatocyte transdifferentiation as the default mechanism of hepatocyte recovery in late larval stage zebrafish. Overall design: We conducted single cell RNA Sequencing using the 10x platform of larval zebrafish liver. This was performed in the basal state Control1  Control2  post partial ablation MTZ 0dppa  MTZ 1dppa and MTZ 9dppa and post partial hepatectomy PHx 4dpi and PHx 11dpi. The two control samples were not treated with 4 OHT  and were also not injured. The control samples are from 13 dpf zebrafish raised under normal rearing condition. For all experiments Tgfabp10a:CellCousin line was used.", null, "pubmed:40480975", null, "Liver MTZ 1dppa", "GSM8403317", null, "source name:Liver|tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Partial Ablation|geo loc name:missing|collection date:missing", "Liver MTZ 1dppa", "A custom Cell Ranger index was generated from the Ensembl GRCz11 genome sequence and annotation filtered with \u2018mkgtf\u2019 command of Cell Ranger options: \u2018\u2014attribute = gene biotype:lincRNA \u2013attribute = gene biotype:antisense  as well as the pseudo mTagBFP2  H2B mGL  mCherry NTR sequences in these cells using the mkref command. This index was used to map reads and generate gene expression matrices using the \u2018count\u2019 command of the Cell Ranger software v.7.1.0 provided by 10x Genomics with the option \u201c\u2013include  introns\u201d set to False all other options were used as per default. Assembly: GRCz11 Supplementary files format and content: Count Matrix as h5ad file Supplementary files format and content: Metadata listing the cell annotations as csv file", "Liver", null, "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer\u2019s instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell 3\u2019 v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer\u2019s protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Partial Ablation", "GSM8403317", "GSM8403317: Liver MTZ 1dppa; Danio rerio; RNA Seq", "GSM8403317 r1", "GSM8403317", "1", "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer's instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell three prime v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer's protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 550", null, "SRP520597", null, "loader:fastq load.py", "ULB-CB-cr-zf-EES2-reload-EES3-1dppa-GEM-240122_S18_L002_I1_001.fastq.gz ULB-CB-cr-zf-EES2-reload-EES3-1dppa-GEM-240122_S18_L002_I2_001.fastq.gz ULB-CB-cr-zf-EES2-reload-EES3-1dppa-GEM-240122_S18_L002_R1_001.fastq.gz ULB-CB-cr-zf-EES2-reload-EES3-1dppa-GEM-240122_S18_L002_R2_001.fastq.gz", "fastq fastq fastq fastq", 21662633664.0, 128944248.0, "GSM8403317 r2", "0:10 1:10 2:28 3:120", "A:4553543446;C:3422125565;G:3539342477;T:3957989911;N:308361", 10, 10, 28, 120, 4553543446, 3422125565, 3539342477, 3957989911, 308361, "SRX25365554", "SRS22031294", "SRA1926595", "Regeneration and Stress Biology, IRIBHM, ULB", "Regeneration and Stress Biology, IRIBHM, ULB", 1, 0.93227, null, 0.07246, null, 0.81696, null, 0.50066, null, 120, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "Belgium", "2024-07-17", "Undetermined", "Larval", "Liver", "Liver and Biliary System"], [33228, "SRR29868321", "SRX25365554", "SRS22031294", "SRP520597", "PRJNA1136957", "Cholangiocytes contribute to hepatocyte regeneration post partial liver injury in larval zebrafish", "GSE272484", "Transcriptome Analysis", "Liver possesses robust regenerative ability  characterized by flexibility in the cellular source of regeneration based on the extent of the injury. post partial hepatectomy or minor injuries  hepatocytes  the primary liver cells  undergo self duplication to replenish the liver mass. In contrast  when the damage is extensive  or hepatocyte proliferation is impaired  cholangiocytes contribute to hepatocyte recovery. This current paradigm of regenerative flexibility in the liver has been established for animals with little or no growth. However  the regenerative mechanisms during periods of growth in young animals remain unexplored. Here  we establish two new partial liver injury protocols in the zebrafish model of rapid growth during late larval stage and observe emergence of de novo hepatocytes in the presence of spared hepatocytes. Using single cell RNA sequencing and lineage tracing  we identify cholangiocytes as the source of de novo hepatocytes. Our study offers a new perspective on the current paradigm of liver regenerating by proposing cholangiocyte to hepatocyte transdifferentiation as the default mechanism of hepatocyte recovery in late larval stage zebrafish. Overall design: We conducted single cell RNA Sequencing using the 10x platform of larval zebrafish liver. This was performed in the basal state Control1  Control2  post partial ablation MTZ 0dppa  MTZ 1dppa and MTZ 9dppa and post partial hepatectomy PHx 4dpi and PHx 11dpi. The two control samples were not treated with 4 OHT  and were also not injured. The control samples are from 13 dpf zebrafish raised under normal rearing condition. For all experiments Tgfabp10a:CellCousin line was used.", null, "pubmed:40480975", null, "Liver MTZ 1dppa", "GSM8403317", null, "source name:Liver|tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Partial Ablation|geo loc name:missing|collection date:missing", "Liver MTZ 1dppa", "A custom Cell Ranger index was generated from the Ensembl GRCz11 genome sequence and annotation filtered with \u2018mkgtf\u2019 command of Cell Ranger options: \u2018\u2014attribute = gene biotype:lincRNA \u2013attribute = gene biotype:antisense  as well as the pseudo mTagBFP2  H2B mGL  mCherry NTR sequences in these cells using the mkref command. This index was used to map reads and generate gene expression matrices using the \u2018count\u2019 command of the Cell Ranger software v.7.1.0 provided by 10x Genomics with the option \u201c\u2013include  introns\u201d set to False all other options were used as per default. Assembly: GRCz11 Supplementary files format and content: Count Matrix as h5ad file Supplementary files format and content: Metadata listing the cell annotations as csv file", "Liver", null, "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer\u2019s instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell 3\u2019 v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer\u2019s protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Partial Ablation", "GSM8403317", "GSM8403317: Liver MTZ 1dppa; Danio rerio; RNA Seq", "GSM8403317 r1", "GSM8403317", "1", "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer's instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell three prime v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer's protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 550", null, "SRP520597", null, "loader:fastq load.py", "ULB-CB-cr-zf-EES2-reload-EES3-1dppa-GEM-240122_S18_L003_R2_001.fastq.gz ULB-CB-cr-zf-EES2-reload-EES3-1dppa-GEM-240122_S18_L003_R1_001.fastq.gz ULB-CB-cr-zf-EES2-reload-EES3-1dppa-GEM-240122_S18_L003_I2_001.fastq.gz ULB-CB-cr-zf-EES2-reload-EES3-1dppa-GEM-240122_S18_L003_I1_001.fastq.gz", "fastq fastq fastq fastq", 22267198800.0, 132542850.0, "GSM8403317 r3", "0:10 1:10 2:28 3:120", "A:4677084299;C:3522052285;G:3641789558;T:4063831592;N:384266", 10, 10, 28, 120, 4677084299, 3522052285, 3641789558, 4063831592, 384266, "SRX25365554", "SRS22031294", "SRA1926595", "Regeneration and Stress Biology, IRIBHM, ULB", "Regeneration and Stress Biology, IRIBHM, ULB", 1, 0.93308, null, 0.07402, null, 0.82035, null, 0.58114, null, 120, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "Belgium", "2024-07-17", "Undetermined", "Larval", "Liver", "Liver and Biliary System"], [33229, "SRR29868322", "SRX25365554", "SRS22031294", "SRP520597", "PRJNA1136957", "Cholangiocytes contribute to hepatocyte regeneration post partial liver injury in larval zebrafish", "GSE272484", "Transcriptome Analysis", "Liver possesses robust regenerative ability  characterized by flexibility in the cellular source of regeneration based on the extent of the injury. post partial hepatectomy or minor injuries  hepatocytes  the primary liver cells  undergo self duplication to replenish the liver mass. In contrast  when the damage is extensive  or hepatocyte proliferation is impaired  cholangiocytes contribute to hepatocyte recovery. This current paradigm of regenerative flexibility in the liver has been established for animals with little or no growth. However  the regenerative mechanisms during periods of growth in young animals remain unexplored. Here  we establish two new partial liver injury protocols in the zebrafish model of rapid growth during late larval stage and observe emergence of de novo hepatocytes in the presence of spared hepatocytes. Using single cell RNA sequencing and lineage tracing  we identify cholangiocytes as the source of de novo hepatocytes. Our study offers a new perspective on the current paradigm of liver regenerating by proposing cholangiocyte to hepatocyte transdifferentiation as the default mechanism of hepatocyte recovery in late larval stage zebrafish. Overall design: We conducted single cell RNA Sequencing using the 10x platform of larval zebrafish liver. This was performed in the basal state Control1  Control2  post partial ablation MTZ 0dppa  MTZ 1dppa and MTZ 9dppa and post partial hepatectomy PHx 4dpi and PHx 11dpi. The two control samples were not treated with 4 OHT  and were also not injured. The control samples are from 13 dpf zebrafish raised under normal rearing condition. For all experiments Tgfabp10a:CellCousin line was used.", null, "pubmed:40480975", null, "Liver MTZ 1dppa", "GSM8403317", null, "source name:Liver|tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Partial Ablation|geo loc name:missing|collection date:missing", "Liver MTZ 1dppa", "A custom Cell Ranger index was generated from the Ensembl GRCz11 genome sequence and annotation filtered with \u2018mkgtf\u2019 command of Cell Ranger options: \u2018\u2014attribute = gene biotype:lincRNA \u2013attribute = gene biotype:antisense  as well as the pseudo mTagBFP2  H2B mGL  mCherry NTR sequences in these cells using the mkref command. This index was used to map reads and generate gene expression matrices using the \u2018count\u2019 command of the Cell Ranger software v.7.1.0 provided by 10x Genomics with the option \u201c\u2013include  introns\u201d set to False all other options were used as per default. Assembly: GRCz11 Supplementary files format and content: Count Matrix as h5ad file Supplementary files format and content: Metadata listing the cell annotations as csv file", "Liver", null, "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer\u2019s instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell 3\u2019 v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer\u2019s protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Partial Ablation", "GSM8403317", "GSM8403317: Liver MTZ 1dppa; Danio rerio; RNA Seq", "GSM8403317 r1", "GSM8403317", "1", "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer's instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell three prime v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer's protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 550", null, "SRP520597", null, "loader:fastq load.py", "ULB-CB-cr-zf-EES2-reload-EES3-1dppa-GEM-240122_S18_L004_R2_001.fastq.gz ULB-CB-cr-zf-EES2-reload-EES3-1dppa-GEM-240122_S18_L004_R1_001.fastq.gz ULB-CB-cr-zf-EES2-reload-EES3-1dppa-GEM-240122_S18_L004_I2_001.fastq.gz ULB-CB-cr-zf-EES2-reload-EES3-1dppa-GEM-240122_S18_L004_I1_001.fastq.gz", "fastq fastq fastq fastq", 21946980216.0, 130636787.0, "GSM8403317 r4", "0:10 1:10 2:28 3:120", "A:4613356051;C:3469396471;G:3586452720;T:4006684161;N:525037", 10, 10, 28, 120, 4613356051, 3469396471, 3586452720, 4006684161, 525037, "SRX25365554", "SRS22031294", "SRA1926595", "Regeneration and Stress Biology, IRIBHM, ULB", "Regeneration and Stress Biology, IRIBHM, ULB", 1, 0.932, null, 0.07454, null, 0.8199, null, 0.57856, null, 120, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "Belgium", "2024-07-17", "Undetermined", "Larval", "Liver", "Liver and Biliary System"], [33230, "SRR29868307", "SRX25365553", "SRS22031298", "SRP520597", "PRJNA1136957", "Cholangiocytes contribute to hepatocyte regeneration post partial liver injury in larval zebrafish", "GSE272484", "Transcriptome Analysis", "Liver possesses robust regenerative ability  characterized by flexibility in the cellular source of regeneration based on the extent of the injury. post partial hepatectomy or minor injuries  hepatocytes  the primary liver cells  undergo self duplication to replenish the liver mass. In contrast  when the damage is extensive  or hepatocyte proliferation is impaired  cholangiocytes contribute to hepatocyte recovery. This current paradigm of regenerative flexibility in the liver has been established for animals with little or no growth. However  the regenerative mechanisms during periods of growth in young animals remain unexplored. Here  we establish two new partial liver injury protocols in the zebrafish model of rapid growth during late larval stage and observe emergence of de novo hepatocytes in the presence of spared hepatocytes. Using single cell RNA sequencing and lineage tracing  we identify cholangiocytes as the source of de novo hepatocytes. Our study offers a new perspective on the current paradigm of liver regenerating by proposing cholangiocyte to hepatocyte transdifferentiation as the default mechanism of hepatocyte recovery in late larval stage zebrafish. Overall design: We conducted single cell RNA Sequencing using the 10x platform of larval zebrafish liver. This was performed in the basal state Control1  Control2  post partial ablation MTZ 0dppa  MTZ 1dppa and MTZ 9dppa and post partial hepatectomy PHx 4dpi and PHx 11dpi. The two control samples were not treated with 4 OHT  and were also not injured. The control samples are from 13 dpf zebrafish raised under normal rearing condition. For all experiments Tgfabp10a:CellCousin line was used.", null, "pubmed:40480975", null, "Liver Control2", "GSM8403315", null, "source name:Liver|tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Control \u2013 No 4 OHT|geo loc name:missing|collection date:missing", "Liver Control2", "A custom Cell Ranger index was generated from the Ensembl GRCz11 genome sequence and annotation filtered with \u2018mkgtf\u2019 command of Cell Ranger options: \u2018\u2014attribute = gene biotype:lincRNA \u2013attribute = gene biotype:antisense  as well as the pseudo mTagBFP2  H2B mGL  mCherry NTR sequences in these cells using the mkref command. This index was used to map reads and generate gene expression matrices using the \u2018count\u2019 command of the Cell Ranger software v.7.1.0 provided by 10x Genomics with the option \u201c\u2013include  introns\u201d set to False all other options were used as per default. Assembly: GRCz11 Supplementary files format and content: Count Matrix as h5ad file Supplementary files format and content: Metadata listing the cell annotations as csv file", "Liver", null, "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer\u2019s instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell 3\u2019 v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer\u2019s protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Control \u2013 No 4 OHT", "GSM8403315", "GSM8403315: Liver Control2; Danio rerio; RNA Seq", "GSM8403315 r1", "GSM8403315", "1", "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer's instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell three prime v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer's protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 550", null, "SRP520597", null, "loader:fastq load.py", "ULB-CB-cr-zf-EES2-larvalliver-CTL213dpf-GEM-230727_S11_L001_R2_001.fastq.gz ULB-CB-cr-zf-EES2-larvalliver-CTL213dpf-GEM-230727_S11_L001_R1_001.fastq.gz ULB-CB-cr-zf-EES2-larvalliver-CTL213dpf-GEM-230727_S11_L001_I2_001.fastq.gz ULB-CB-cr-zf-EES2-larvalliver-CTL213dpf-GEM-230727_S11_L001_I1_001.fastq.gz", "fastq fastq fastq fastq", 9879931488.0, 58809116.0, "GSM8403315 r1", "0:10 1:10 2:28 3:120", "A:2083561937;C:1579881891;G:1601331938;T:1792234199;N:83955", 10, 10, 28, 120, 2083561937, 1579881891, 1601331938, 1792234199, 83955, "SRX25365553", "SRS22031298", "SRA1926595", "Regeneration and Stress Biology, IRIBHM, ULB", "Regeneration and Stress Biology, IRIBHM, ULB", 1, 0.9245, null, 0.07832, null, 0.83207, null, 0.59038, null, 120, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "Belgium", "2024-07-17", "Undetermined", "Larval", "Liver", "Liver and Biliary System"], [33231, "SRR29868308", "SRX25365553", "SRS22031298", "SRP520597", "PRJNA1136957", "Cholangiocytes contribute to hepatocyte regeneration post partial liver injury in larval zebrafish", "GSE272484", "Transcriptome Analysis", "Liver possesses robust regenerative ability  characterized by flexibility in the cellular source of regeneration based on the extent of the injury. post partial hepatectomy or minor injuries  hepatocytes  the primary liver cells  undergo self duplication to replenish the liver mass. In contrast  when the damage is extensive  or hepatocyte proliferation is impaired  cholangiocytes contribute to hepatocyte recovery. This current paradigm of regenerative flexibility in the liver has been established for animals with little or no growth. However  the regenerative mechanisms during periods of growth in young animals remain unexplored. Here  we establish two new partial liver injury protocols in the zebrafish model of rapid growth during late larval stage and observe emergence of de novo hepatocytes in the presence of spared hepatocytes. Using single cell RNA sequencing and lineage tracing  we identify cholangiocytes as the source of de novo hepatocytes. Our study offers a new perspective on the current paradigm of liver regenerating by proposing cholangiocyte to hepatocyte transdifferentiation as the default mechanism of hepatocyte recovery in late larval stage zebrafish. Overall design: We conducted single cell RNA Sequencing using the 10x platform of larval zebrafish liver. This was performed in the basal state Control1  Control2  post partial ablation MTZ 0dppa  MTZ 1dppa and MTZ 9dppa and post partial hepatectomy PHx 4dpi and PHx 11dpi. The two control samples were not treated with 4 OHT  and were also not injured. The control samples are from 13 dpf zebrafish raised under normal rearing condition. For all experiments Tgfabp10a:CellCousin line was used.", null, "pubmed:40480975", null, "Liver Control2", "GSM8403315", null, "source name:Liver|tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Control \u2013 No 4 OHT|geo loc name:missing|collection date:missing", "Liver Control2", "A custom Cell Ranger index was generated from the Ensembl GRCz11 genome sequence and annotation filtered with \u2018mkgtf\u2019 command of Cell Ranger options: \u2018\u2014attribute = gene biotype:lincRNA \u2013attribute = gene biotype:antisense  as well as the pseudo mTagBFP2  H2B mGL  mCherry NTR sequences in these cells using the mkref command. This index was used to map reads and generate gene expression matrices using the \u2018count\u2019 command of the Cell Ranger software v.7.1.0 provided by 10x Genomics with the option \u201c\u2013include  introns\u201d set to False all other options were used as per default. Assembly: GRCz11 Supplementary files format and content: Count Matrix as h5ad file Supplementary files format and content: Metadata listing the cell annotations as csv file", "Liver", null, "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer\u2019s instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell 3\u2019 v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer\u2019s protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Control \u2013 No 4 OHT", "GSM8403315", "GSM8403315: Liver Control2; Danio rerio; RNA Seq", "GSM8403315 r1", "GSM8403315", "1", "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer's instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell three prime v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer's protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 550", null, "SRP520597", null, "loader:fastq load.py", "ULB-CB-cr-zf-EES4-reload-EES2-larvlivCTL213dpf-GEM-230727_S20_L001_R2_001.fastq.gz ULB-CB-cr-zf-EES4-reload-EES2-larvlivCTL213dpf-GEM-230727_S20_L001_R1_001.fastq.gz ULB-CB-cr-zf-EES4-reload-EES2-larvlivCTL213dpf-GEM-230727_S20_L001_I2_001.fastq.gz ULB-CB-cr-zf-EES4-reload-EES2-larvlivCTL213dpf-GEM-230727_S20_L001_I1_001.fastq.gz", "fastq fastq fastq fastq", 11728809288.0, 69814341.0, "GSM8403315 r5", "0:10 1:10 2:28 3:120", "A:2469898525;C:1869748167;G:1900689647;T:2137258339;N:126242", 10, 10, 28, 120, 2469898525, 1869748167, 1900689647, 2137258339, 126242, "SRX25365553", "SRS22031298", "SRA1926595", "Regeneration and Stress Biology, IRIBHM, ULB", "Regeneration and Stress Biology, IRIBHM, ULB", 1, 0.92438, null, 0.08084, null, 0.83181, null, 0.58855, null, 120, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "Belgium", "2024-07-17", "Undetermined", "Larval", "Liver", "Liver and Biliary System"], [33232, "SRR29868309", "SRX25365553", "SRS22031298", "SRP520597", "PRJNA1136957", "Cholangiocytes contribute to hepatocyte regeneration post partial liver injury in larval zebrafish", "GSE272484", "Transcriptome Analysis", "Liver possesses robust regenerative ability  characterized by flexibility in the cellular source of regeneration based on the extent of the injury. post partial hepatectomy or minor injuries  hepatocytes  the primary liver cells  undergo self duplication to replenish the liver mass. In contrast  when the damage is extensive  or hepatocyte proliferation is impaired  cholangiocytes contribute to hepatocyte recovery. This current paradigm of regenerative flexibility in the liver has been established for animals with little or no growth. However  the regenerative mechanisms during periods of growth in young animals remain unexplored. Here  we establish two new partial liver injury protocols in the zebrafish model of rapid growth during late larval stage and observe emergence of de novo hepatocytes in the presence of spared hepatocytes. Using single cell RNA sequencing and lineage tracing  we identify cholangiocytes as the source of de novo hepatocytes. Our study offers a new perspective on the current paradigm of liver regenerating by proposing cholangiocyte to hepatocyte transdifferentiation as the default mechanism of hepatocyte recovery in late larval stage zebrafish. Overall design: We conducted single cell RNA Sequencing using the 10x platform of larval zebrafish liver. This was performed in the basal state Control1  Control2  post partial ablation MTZ 0dppa  MTZ 1dppa and MTZ 9dppa and post partial hepatectomy PHx 4dpi and PHx 11dpi. The two control samples were not treated with 4 OHT  and were also not injured. The control samples are from 13 dpf zebrafish raised under normal rearing condition. For all experiments Tgfabp10a:CellCousin line was used.", null, "pubmed:40480975", null, "Liver Control2", "GSM8403315", null, "source name:Liver|tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Control \u2013 No 4 OHT|geo loc name:missing|collection date:missing", "Liver Control2", "A custom Cell Ranger index was generated from the Ensembl GRCz11 genome sequence and annotation filtered with \u2018mkgtf\u2019 command of Cell Ranger options: \u2018\u2014attribute = gene biotype:lincRNA \u2013attribute = gene biotype:antisense  as well as the pseudo mTagBFP2  H2B mGL  mCherry NTR sequences in these cells using the mkref command. This index was used to map reads and generate gene expression matrices using the \u2018count\u2019 command of the Cell Ranger software v.7.1.0 provided by 10x Genomics with the option \u201c\u2013include  introns\u201d set to False all other options were used as per default. Assembly: GRCz11 Supplementary files format and content: Count Matrix as h5ad file Supplementary files format and content: Metadata listing the cell annotations as csv file", "Liver", null, "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer\u2019s instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell 3\u2019 v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer\u2019s protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Control \u2013 No 4 OHT", "GSM8403315", "GSM8403315: Liver Control2; Danio rerio; RNA Seq", "GSM8403315 r1", "GSM8403315", "1", "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer's instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell three prime v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer's protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 550", null, "SRP520597", null, "loader:fastq load.py", "ULB-CB-cr-zf-EES4-reload-EES2-larvlivCTL213dpf-GEM-230727_S20_L002_R2_001.fastq.gz ULB-CB-cr-zf-EES4-reload-EES2-larvlivCTL213dpf-GEM-230727_S20_L002_R1_001.fastq.gz ULB-CB-cr-zf-EES4-reload-EES2-larvlivCTL213dpf-GEM-230727_S20_L002_I2_001.fastq.gz ULB-CB-cr-zf-EES4-reload-EES2-larvlivCTL213dpf-GEM-230727_S20_L002_I1_001.fastq.gz", "fastq fastq fastq fastq", 11877483744.0, 70699308.0, "GSM8403315 r6", "0:10 1:10 2:28 3:120", "A:2501780371;C:1893908685;G:1923797061;T:2164259893;N:170950", 10, 10, 28, 120, 2501780371, 1893908685, 1923797061, 2164259893, 170950, "SRX25365553", "SRS22031298", "SRA1926595", "Regeneration and Stress Biology, IRIBHM, ULB", "Regeneration and Stress Biology, IRIBHM, ULB", 1, 0.92477, null, 0.07874, null, 0.83321, null, 0.5933, null, 120, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "Belgium", "2024-07-17", "Undetermined", "Larval", "Liver", "Liver and Biliary System"], [33233, "SRR29868310", "SRX25365553", "SRS22031298", "SRP520597", "PRJNA1136957", "Cholangiocytes contribute to hepatocyte regeneration post partial liver injury in larval zebrafish", "GSE272484", "Transcriptome Analysis", "Liver possesses robust regenerative ability  characterized by flexibility in the cellular source of regeneration based on the extent of the injury. post partial hepatectomy or minor injuries  hepatocytes  the primary liver cells  undergo self duplication to replenish the liver mass. In contrast  when the damage is extensive  or hepatocyte proliferation is impaired  cholangiocytes contribute to hepatocyte recovery. This current paradigm of regenerative flexibility in the liver has been established for animals with little or no growth. However  the regenerative mechanisms during periods of growth in young animals remain unexplored. Here  we establish two new partial liver injury protocols in the zebrafish model of rapid growth during late larval stage and observe emergence of de novo hepatocytes in the presence of spared hepatocytes. Using single cell RNA sequencing and lineage tracing  we identify cholangiocytes as the source of de novo hepatocytes. Our study offers a new perspective on the current paradigm of liver regenerating by proposing cholangiocyte to hepatocyte transdifferentiation as the default mechanism of hepatocyte recovery in late larval stage zebrafish. Overall design: We conducted single cell RNA Sequencing using the 10x platform of larval zebrafish liver. This was performed in the basal state Control1  Control2  post partial ablation MTZ 0dppa  MTZ 1dppa and MTZ 9dppa and post partial hepatectomy PHx 4dpi and PHx 11dpi. The two control samples were not treated with 4 OHT  and were also not injured. The control samples are from 13 dpf zebrafish raised under normal rearing condition. For all experiments Tgfabp10a:CellCousin line was used.", null, "pubmed:40480975", null, "Liver Control2", "GSM8403315", null, "source name:Liver|tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Control \u2013 No 4 OHT|geo loc name:missing|collection date:missing", "Liver Control2", "A custom Cell Ranger index was generated from the Ensembl GRCz11 genome sequence and annotation filtered with \u2018mkgtf\u2019 command of Cell Ranger options: \u2018\u2014attribute = gene biotype:lincRNA \u2013attribute = gene biotype:antisense  as well as the pseudo mTagBFP2  H2B mGL  mCherry NTR sequences in these cells using the mkref command. This index was used to map reads and generate gene expression matrices using the \u2018count\u2019 command of the Cell Ranger software v.7.1.0 provided by 10x Genomics with the option \u201c\u2013include  introns\u201d set to False all other options were used as per default. Assembly: GRCz11 Supplementary files format and content: Count Matrix as h5ad file Supplementary files format and content: Metadata listing the cell annotations as csv file", "Liver", null, "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer\u2019s instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell 3\u2019 v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer\u2019s protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Control \u2013 No 4 OHT", "GSM8403315", "GSM8403315: Liver Control2; Danio rerio; RNA Seq", "GSM8403315 r1", "GSM8403315", "1", "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer's instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell three prime v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer's protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 550", null, "SRP520597", null, "loader:fastq load.py", "ULB-CB-cr-zf-EES4-reload-EES2-larvlivCTL213dpf-GEM-230727_S20_L003_R2_001.fastq.gz ULB-CB-cr-zf-EES4-reload-EES2-larvlivCTL213dpf-GEM-230727_S20_L003_R1_001.fastq.gz ULB-CB-cr-zf-EES4-reload-EES2-larvlivCTL213dpf-GEM-230727_S20_L003_I2_001.fastq.gz ULB-CB-cr-zf-EES4-reload-EES2-larvlivCTL213dpf-GEM-230727_S20_L003_I1_001.fastq.gz", "fastq fastq fastq fastq", 12148826256.0, 72314442.0, "GSM8403315 r7", "0:10 1:10 2:28 3:120", "A:2557348707;C:1939726759;G:1969388742;T:2211056576;N:212256", 10, 10, 28, 120, 2557348707, 1939726759, 1969388742, 2211056576, 212256, "SRX25365553", "SRS22031298", "SRA1926595", "Regeneration and Stress Biology, IRIBHM, ULB", "Regeneration and Stress Biology, IRIBHM, ULB", 1, 0.9241, null, 0.07899, null, 0.83246, null, 0.59733, null, 120, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "Belgium", "2024-07-17", "Undetermined", "Larval", "Liver", "Liver and Biliary System"], [33234, "SRR29868311", "SRX25365553", "SRS22031298", "SRP520597", "PRJNA1136957", "Cholangiocytes contribute to hepatocyte regeneration post partial liver injury in larval zebrafish", "GSE272484", "Transcriptome Analysis", "Liver possesses robust regenerative ability  characterized by flexibility in the cellular source of regeneration based on the extent of the injury. post partial hepatectomy or minor injuries  hepatocytes  the primary liver cells  undergo self duplication to replenish the liver mass. In contrast  when the damage is extensive  or hepatocyte proliferation is impaired  cholangiocytes contribute to hepatocyte recovery. This current paradigm of regenerative flexibility in the liver has been established for animals with little or no growth. However  the regenerative mechanisms during periods of growth in young animals remain unexplored. Here  we establish two new partial liver injury protocols in the zebrafish model of rapid growth during late larval stage and observe emergence of de novo hepatocytes in the presence of spared hepatocytes. Using single cell RNA sequencing and lineage tracing  we identify cholangiocytes as the source of de novo hepatocytes. Our study offers a new perspective on the current paradigm of liver regenerating by proposing cholangiocyte to hepatocyte transdifferentiation as the default mechanism of hepatocyte recovery in late larval stage zebrafish. Overall design: We conducted single cell RNA Sequencing using the 10x platform of larval zebrafish liver. This was performed in the basal state Control1  Control2  post partial ablation MTZ 0dppa  MTZ 1dppa and MTZ 9dppa and post partial hepatectomy PHx 4dpi and PHx 11dpi. The two control samples were not treated with 4 OHT  and were also not injured. The control samples are from 13 dpf zebrafish raised under normal rearing condition. For all experiments Tgfabp10a:CellCousin line was used.", null, "pubmed:40480975", null, "Liver Control2", "GSM8403315", null, "source name:Liver|tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Control \u2013 No 4 OHT|geo loc name:missing|collection date:missing", "Liver Control2", "A custom Cell Ranger index was generated from the Ensembl GRCz11 genome sequence and annotation filtered with \u2018mkgtf\u2019 command of Cell Ranger options: \u2018\u2014attribute = gene biotype:lincRNA \u2013attribute = gene biotype:antisense  as well as the pseudo mTagBFP2  H2B mGL  mCherry NTR sequences in these cells using the mkref command. This index was used to map reads and generate gene expression matrices using the \u2018count\u2019 command of the Cell Ranger software v.7.1.0 provided by 10x Genomics with the option \u201c\u2013include  introns\u201d set to False all other options were used as per default. Assembly: GRCz11 Supplementary files format and content: Count Matrix as h5ad file Supplementary files format and content: Metadata listing the cell annotations as csv file", "Liver", null, "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer\u2019s instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell 3\u2019 v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer\u2019s protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Control \u2013 No 4 OHT", "GSM8403315", "GSM8403315: Liver Control2; Danio rerio; RNA Seq", "GSM8403315 r1", "GSM8403315", "1", "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer's instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell three prime v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer's protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 550", null, "SRP520597", null, "loader:fastq load.py", "ULB-CB-cr-zf-EES4-reload-EES2-larvlivCTL213dpf-GEM-230727_S20_L004_R2_001.fastq.gz ULB-CB-cr-zf-EES4-reload-EES2-larvlivCTL213dpf-GEM-230727_S20_L004_R1_001.fastq.gz ULB-CB-cr-zf-EES4-reload-EES2-larvlivCTL213dpf-GEM-230727_S20_L004_I2_001.fastq.gz ULB-CB-cr-zf-EES4-reload-EES2-larvlivCTL213dpf-GEM-230727_S20_L004_I1_001.fastq.gz", "fastq fastq fastq fastq", 12000520224.0, 71431668.0, "GSM8403315 r8", "0:10 1:10 2:28 3:120", "A:2527907019;C:1914860315;G:1943708096;T:2185034081;N:290649", 10, 10, 28, 120, 2527907019, 1914860315, 1943708096, 2185034081, 290649, "SRX25365553", "SRS22031298", "SRA1926595", "Regeneration and Stress Biology, IRIBHM, ULB", "Regeneration and Stress Biology, IRIBHM, ULB", 1, 0.92467, null, 0.07869, null, 0.83274, null, 0.59327, null, 120, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "Belgium", "2024-07-17", "Undetermined", "Larval", "Liver", "Liver and Biliary System"], [33235, "SRR29868331", "SRX25365553", "SRS22031298", "SRP520597", "PRJNA1136957", "Cholangiocytes contribute to hepatocyte regeneration post partial liver injury in larval zebrafish", "GSE272484", "Transcriptome Analysis", "Liver possesses robust regenerative ability  characterized by flexibility in the cellular source of regeneration based on the extent of the injury. post partial hepatectomy or minor injuries  hepatocytes  the primary liver cells  undergo self duplication to replenish the liver mass. In contrast  when the damage is extensive  or hepatocyte proliferation is impaired  cholangiocytes contribute to hepatocyte recovery. This current paradigm of regenerative flexibility in the liver has been established for animals with little or no growth. However  the regenerative mechanisms during periods of growth in young animals remain unexplored. Here  we establish two new partial liver injury protocols in the zebrafish model of rapid growth during late larval stage and observe emergence of de novo hepatocytes in the presence of spared hepatocytes. Using single cell RNA sequencing and lineage tracing  we identify cholangiocytes as the source of de novo hepatocytes. Our study offers a new perspective on the current paradigm of liver regenerating by proposing cholangiocyte to hepatocyte transdifferentiation as the default mechanism of hepatocyte recovery in late larval stage zebrafish. Overall design: We conducted single cell RNA Sequencing using the 10x platform of larval zebrafish liver. This was performed in the basal state Control1  Control2  post partial ablation MTZ 0dppa  MTZ 1dppa and MTZ 9dppa and post partial hepatectomy PHx 4dpi and PHx 11dpi. The two control samples were not treated with 4 OHT  and were also not injured. The control samples are from 13 dpf zebrafish raised under normal rearing condition. For all experiments Tgfabp10a:CellCousin line was used.", null, "pubmed:40480975", null, "Liver Control2", "GSM8403315", null, "source name:Liver|tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Control \u2013 No 4 OHT|geo loc name:missing|collection date:missing", "Liver Control2", "A custom Cell Ranger index was generated from the Ensembl GRCz11 genome sequence and annotation filtered with \u2018mkgtf\u2019 command of Cell Ranger options: \u2018\u2014attribute = gene biotype:lincRNA \u2013attribute = gene biotype:antisense  as well as the pseudo mTagBFP2  H2B mGL  mCherry NTR sequences in these cells using the mkref command. This index was used to map reads and generate gene expression matrices using the \u2018count\u2019 command of the Cell Ranger software v.7.1.0 provided by 10x Genomics with the option \u201c\u2013include  introns\u201d set to False all other options were used as per default. Assembly: GRCz11 Supplementary files format and content: Count Matrix as h5ad file Supplementary files format and content: Metadata listing the cell annotations as csv file", "Liver", null, "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer\u2019s instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell 3\u2019 v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer\u2019s protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Control \u2013 No 4 OHT", "GSM8403315", "GSM8403315: Liver Control2; Danio rerio; RNA Seq", "GSM8403315 r1", "GSM8403315", "1", "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer's instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell three prime v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer's protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 550", null, "SRP520597", null, "loader:fastq load.py", "ULB-CB-cr-zf-EES2-larvalliver-CTL213dpf-GEM-230727_S11_L002_I1_001.fastq.gz ULB-CB-cr-zf-EES2-larvalliver-CTL213dpf-GEM-230727_S11_L002_I2_001.fastq.gz ULB-CB-cr-zf-EES2-larvalliver-CTL213dpf-GEM-230727_S11_L002_R1_001.fastq.gz ULB-CB-cr-zf-EES2-larvalliver-CTL213dpf-GEM-230727_S11_L002_R2_001.fastq.gz", "fastq fastq fastq fastq", 10005892344.0, 59558883.0, "GSM8403315 r2", "0:10 1:10 2:28 3:120", "A:2111541927;C:1599222457;G:1620308600;T:1815908968;N:84008", 10, 10, 28, 120, 2111541927, 1599222457, 1620308600, 1815908968, 84008, "SRX25365553", "SRS22031298", "SRA1926595", "Regeneration and Stress Biology, IRIBHM, ULB", "Regeneration and Stress Biology, IRIBHM, ULB", 1, 0.925, null, 0.07865, null, 0.83435, null, 0.60287, null, 120, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "Belgium", "2024-07-17", "Undetermined", "Larval", "Liver", "Liver and Biliary System"], [33236, "SRR29868332", "SRX25365553", "SRS22031298", "SRP520597", "PRJNA1136957", "Cholangiocytes contribute to hepatocyte regeneration post partial liver injury in larval zebrafish", "GSE272484", "Transcriptome Analysis", "Liver possesses robust regenerative ability  characterized by flexibility in the cellular source of regeneration based on the extent of the injury. post partial hepatectomy or minor injuries  hepatocytes  the primary liver cells  undergo self duplication to replenish the liver mass. In contrast  when the damage is extensive  or hepatocyte proliferation is impaired  cholangiocytes contribute to hepatocyte recovery. This current paradigm of regenerative flexibility in the liver has been established for animals with little or no growth. However  the regenerative mechanisms during periods of growth in young animals remain unexplored. Here  we establish two new partial liver injury protocols in the zebrafish model of rapid growth during late larval stage and observe emergence of de novo hepatocytes in the presence of spared hepatocytes. Using single cell RNA sequencing and lineage tracing  we identify cholangiocytes as the source of de novo hepatocytes. Our study offers a new perspective on the current paradigm of liver regenerating by proposing cholangiocyte to hepatocyte transdifferentiation as the default mechanism of hepatocyte recovery in late larval stage zebrafish. Overall design: We conducted single cell RNA Sequencing using the 10x platform of larval zebrafish liver. This was performed in the basal state Control1  Control2  post partial ablation MTZ 0dppa  MTZ 1dppa and MTZ 9dppa and post partial hepatectomy PHx 4dpi and PHx 11dpi. The two control samples were not treated with 4 OHT  and were also not injured. The control samples are from 13 dpf zebrafish raised under normal rearing condition. For all experiments Tgfabp10a:CellCousin line was used.", null, "pubmed:40480975", null, "Liver Control2", "GSM8403315", null, "source name:Liver|tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Control \u2013 No 4 OHT|geo loc name:missing|collection date:missing", "Liver Control2", "A custom Cell Ranger index was generated from the Ensembl GRCz11 genome sequence and annotation filtered with \u2018mkgtf\u2019 command of Cell Ranger options: \u2018\u2014attribute = gene biotype:lincRNA \u2013attribute = gene biotype:antisense  as well as the pseudo mTagBFP2  H2B mGL  mCherry NTR sequences in these cells using the mkref command. This index was used to map reads and generate gene expression matrices using the \u2018count\u2019 command of the Cell Ranger software v.7.1.0 provided by 10x Genomics with the option \u201c\u2013include  introns\u201d set to False all other options were used as per default. Assembly: GRCz11 Supplementary files format and content: Count Matrix as h5ad file Supplementary files format and content: Metadata listing the cell annotations as csv file", "Liver", null, "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer\u2019s instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell 3\u2019 v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer\u2019s protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Control \u2013 No 4 OHT", "GSM8403315", "GSM8403315: Liver Control2; Danio rerio; RNA Seq", "GSM8403315 r1", "GSM8403315", "1", "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer's instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell three prime v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer's protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 550", null, "SRP520597", null, "loader:fastq load.py", "ULB-CB-cr-zf-EES2-larvalliver-CTL213dpf-GEM-230727_S11_L003_I1_001.fastq.gz ULB-CB-cr-zf-EES2-larvalliver-CTL213dpf-GEM-230727_S11_L003_I2_001.fastq.gz ULB-CB-cr-zf-EES2-larvalliver-CTL213dpf-GEM-230727_S11_L003_R1_001.fastq.gz ULB-CB-cr-zf-EES2-larvalliver-CTL213dpf-GEM-230727_S11_L003_R2_001.fastq.gz", "fastq fastq fastq fastq", 10016183856.0, 59620142.0, "GSM8403315 r3", "0:10 1:10 2:28 3:120", "A:2111419802;C:1602592297;G:1623486867;T:1816842279;N:75795", 10, 10, 28, 120, 2111419802, 1602592297, 1623486867, 1816842279, 75795, "SRX25365553", "SRS22031298", "SRA1926595", "Regeneration and Stress Biology, IRIBHM, ULB", "Regeneration and Stress Biology, IRIBHM, ULB", 1, 0.9252, null, 0.07706, null, 0.83311, null, 0.58482, null, 120, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "Belgium", "2024-07-17", "Undetermined", "Larval", "Liver", "Liver and Biliary System"], [33237, "SRR29868333", "SRX25365553", "SRS22031298", "SRP520597", "PRJNA1136957", "Cholangiocytes contribute to hepatocyte regeneration post partial liver injury in larval zebrafish", "GSE272484", "Transcriptome Analysis", "Liver possesses robust regenerative ability  characterized by flexibility in the cellular source of regeneration based on the extent of the injury. post partial hepatectomy or minor injuries  hepatocytes  the primary liver cells  undergo self duplication to replenish the liver mass. In contrast  when the damage is extensive  or hepatocyte proliferation is impaired  cholangiocytes contribute to hepatocyte recovery. This current paradigm of regenerative flexibility in the liver has been established for animals with little or no growth. However  the regenerative mechanisms during periods of growth in young animals remain unexplored. Here  we establish two new partial liver injury protocols in the zebrafish model of rapid growth during late larval stage and observe emergence of de novo hepatocytes in the presence of spared hepatocytes. Using single cell RNA sequencing and lineage tracing  we identify cholangiocytes as the source of de novo hepatocytes. Our study offers a new perspective on the current paradigm of liver regenerating by proposing cholangiocyte to hepatocyte transdifferentiation as the default mechanism of hepatocyte recovery in late larval stage zebrafish. Overall design: We conducted single cell RNA Sequencing using the 10x platform of larval zebrafish liver. This was performed in the basal state Control1  Control2  post partial ablation MTZ 0dppa  MTZ 1dppa and MTZ 9dppa and post partial hepatectomy PHx 4dpi and PHx 11dpi. The two control samples were not treated with 4 OHT  and were also not injured. The control samples are from 13 dpf zebrafish raised under normal rearing condition. For all experiments Tgfabp10a:CellCousin line was used.", null, "pubmed:40480975", null, "Liver Control2", "GSM8403315", null, "source name:Liver|tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Control \u2013 No 4 OHT|geo loc name:missing|collection date:missing", "Liver Control2", "A custom Cell Ranger index was generated from the Ensembl GRCz11 genome sequence and annotation filtered with \u2018mkgtf\u2019 command of Cell Ranger options: \u2018\u2014attribute = gene biotype:lincRNA \u2013attribute = gene biotype:antisense  as well as the pseudo mTagBFP2  H2B mGL  mCherry NTR sequences in these cells using the mkref command. This index was used to map reads and generate gene expression matrices using the \u2018count\u2019 command of the Cell Ranger software v.7.1.0 provided by 10x Genomics with the option \u201c\u2013include  introns\u201d set to False all other options were used as per default. Assembly: GRCz11 Supplementary files format and content: Count Matrix as h5ad file Supplementary files format and content: Metadata listing the cell annotations as csv file", "Liver", null, "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer\u2019s instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell 3\u2019 v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer\u2019s protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Control \u2013 No 4 OHT", "GSM8403315", "GSM8403315: Liver Control2; Danio rerio; RNA Seq", "GSM8403315 r1", "GSM8403315", "1", "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer's instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell three prime v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer's protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 550", null, "SRP520597", null, "loader:fastq load.py", "ULB-CB-cr-zf-EES2-larvalliver-CTL213dpf-GEM-230727_S11_L004_R2_001.fastq.gz ULB-CB-cr-zf-EES2-larvalliver-CTL213dpf-GEM-230727_S11_L004_R1_001.fastq.gz ULB-CB-cr-zf-EES2-larvalliver-CTL213dpf-GEM-230727_S11_L004_I2_001.fastq.gz ULB-CB-cr-zf-EES2-larvalliver-CTL213dpf-GEM-230727_S11_L004_I1_001.fastq.gz", "fastq fastq fastq fastq", 9972679080.0, 59361185.0, "GSM8403315 r4", "0:10 1:10 2:28 3:120", "A:2102584213;C:1595356295;G:1615800606;T:1809513301;N:87785", 10, 10, 28, 120, 2102584213, 1595356295, 1615800606, 1809513301, 87785, "SRX25365553", "SRS22031298", "SRA1926595", "Regeneration and Stress Biology, IRIBHM, ULB", "Regeneration and Stress Biology, IRIBHM, ULB", 1, 0.92471, null, 0.07908, null, 0.83368, null, 0.59597, null, 120, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "Belgium", "2024-07-17", "Undetermined", "Larval", "Liver", "Liver and Biliary System"], [33238, "SRR29868312", "SRX25365552", "SRS22031293", "SRP520597", "PRJNA1136957", "Cholangiocytes contribute to hepatocyte regeneration post partial liver injury in larval zebrafish", "GSE272484", "Transcriptome Analysis", "Liver possesses robust regenerative ability  characterized by flexibility in the cellular source of regeneration based on the extent of the injury. post partial hepatectomy or minor injuries  hepatocytes  the primary liver cells  undergo self duplication to replenish the liver mass. In contrast  when the damage is extensive  or hepatocyte proliferation is impaired  cholangiocytes contribute to hepatocyte recovery. This current paradigm of regenerative flexibility in the liver has been established for animals with little or no growth. However  the regenerative mechanisms during periods of growth in young animals remain unexplored. Here  we establish two new partial liver injury protocols in the zebrafish model of rapid growth during late larval stage and observe emergence of de novo hepatocytes in the presence of spared hepatocytes. Using single cell RNA sequencing and lineage tracing  we identify cholangiocytes as the source of de novo hepatocytes. Our study offers a new perspective on the current paradigm of liver regenerating by proposing cholangiocyte to hepatocyte transdifferentiation as the default mechanism of hepatocyte recovery in late larval stage zebrafish. Overall design: We conducted single cell RNA Sequencing using the 10x platform of larval zebrafish liver. This was performed in the basal state Control1  Control2  post partial ablation MTZ 0dppa  MTZ 1dppa and MTZ 9dppa and post partial hepatectomy PHx 4dpi and PHx 11dpi. The two control samples were not treated with 4 OHT  and were also not injured. The control samples are from 13 dpf zebrafish raised under normal rearing condition. For all experiments Tgfabp10a:CellCousin line was used.", null, "pubmed:40480975", null, "Liver MTZ 0dppa", "GSM8403316", null, "source name:Liver|tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Partial Ablation|geo loc name:missing|collection date:missing", "Liver MTZ 0dppa", "A custom Cell Ranger index was generated from the Ensembl GRCz11 genome sequence and annotation filtered with \u2018mkgtf\u2019 command of Cell Ranger options: \u2018\u2014attribute = gene biotype:lincRNA \u2013attribute = gene biotype:antisense  as well as the pseudo mTagBFP2  H2B mGL  mCherry NTR sequences in these cells using the mkref command. This index was used to map reads and generate gene expression matrices using the \u2018count\u2019 command of the Cell Ranger software v.7.1.0 provided by 10x Genomics with the option \u201c\u2013include  introns\u201d set to False all other options were used as per default. Assembly: GRCz11 Supplementary files format and content: Count Matrix as h5ad file Supplementary files format and content: Metadata listing the cell annotations as csv file", "Liver", null, "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer\u2019s instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell 3\u2019 v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer\u2019s protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Partial Ablation", "GSM8403316", "GSM8403316: Liver MTZ 0dppa; Danio rerio; RNA Seq", "GSM8403316 r1", "GSM8403316", "1", "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer's instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell three prime v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer's protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 550", null, "SRP520597", null, "loader:fastq load.py", "ULB-CB-cr-zf-EES1-reload-EES2-0dppa-GEM-240122_S17_L001_R2_001.fastq.gz ULB-CB-cr-zf-EES1-reload-EES2-0dppa-GEM-240122_S17_L001_R1_001.fastq.gz ULB-CB-cr-zf-EES1-reload-EES2-0dppa-GEM-240122_S17_L001_I2_001.fastq.gz ULB-CB-cr-zf-EES1-reload-EES2-0dppa-GEM-240122_S17_L001_I1_001.fastq.gz", "fastq fastq fastq fastq", 21831966072.0, 129952179.0, "GSM8403316 r1", "0:10 1:10 2:28 3:120", "A:4479350098;C:3460914438;G:3623235900;T:4030530673;N:230371", 10, 10, 28, 120, 4479350098, 3460914438, 3623235900, 4030530673, 230371, "SRX25365552", "SRS22031293", "SRA1926595", "Regeneration and Stress Biology, IRIBHM, ULB", "Regeneration and Stress Biology, IRIBHM, ULB", 1, 0.93487, null, 0.07462, null, 0.81168, null, 0.58593, null, 120, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "Belgium", "2024-07-17", "Undetermined", "Larval", "Liver", "Liver and Biliary System"], [33239, "SRR29868313", "SRX25365552", "SRS22031293", "SRP520597", "PRJNA1136957", "Cholangiocytes contribute to hepatocyte regeneration post partial liver injury in larval zebrafish", "GSE272484", "Transcriptome Analysis", "Liver possesses robust regenerative ability  characterized by flexibility in the cellular source of regeneration based on the extent of the injury. post partial hepatectomy or minor injuries  hepatocytes  the primary liver cells  undergo self duplication to replenish the liver mass. In contrast  when the damage is extensive  or hepatocyte proliferation is impaired  cholangiocytes contribute to hepatocyte recovery. This current paradigm of regenerative flexibility in the liver has been established for animals with little or no growth. However  the regenerative mechanisms during periods of growth in young animals remain unexplored. Here  we establish two new partial liver injury protocols in the zebrafish model of rapid growth during late larval stage and observe emergence of de novo hepatocytes in the presence of spared hepatocytes. Using single cell RNA sequencing and lineage tracing  we identify cholangiocytes as the source of de novo hepatocytes. Our study offers a new perspective on the current paradigm of liver regenerating by proposing cholangiocyte to hepatocyte transdifferentiation as the default mechanism of hepatocyte recovery in late larval stage zebrafish. Overall design: We conducted single cell RNA Sequencing using the 10x platform of larval zebrafish liver. This was performed in the basal state Control1  Control2  post partial ablation MTZ 0dppa  MTZ 1dppa and MTZ 9dppa and post partial hepatectomy PHx 4dpi and PHx 11dpi. The two control samples were not treated with 4 OHT  and were also not injured. The control samples are from 13 dpf zebrafish raised under normal rearing condition. For all experiments Tgfabp10a:CellCousin line was used.", null, "pubmed:40480975", null, "Liver MTZ 0dppa", "GSM8403316", null, "source name:Liver|tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Partial Ablation|geo loc name:missing|collection date:missing", "Liver MTZ 0dppa", "A custom Cell Ranger index was generated from the Ensembl GRCz11 genome sequence and annotation filtered with \u2018mkgtf\u2019 command of Cell Ranger options: \u2018\u2014attribute = gene biotype:lincRNA \u2013attribute = gene biotype:antisense  as well as the pseudo mTagBFP2  H2B mGL  mCherry NTR sequences in these cells using the mkref command. This index was used to map reads and generate gene expression matrices using the \u2018count\u2019 command of the Cell Ranger software v.7.1.0 provided by 10x Genomics with the option \u201c\u2013include  introns\u201d set to False all other options were used as per default. Assembly: GRCz11 Supplementary files format and content: Count Matrix as h5ad file Supplementary files format and content: Metadata listing the cell annotations as csv file", "Liver", null, "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer\u2019s instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell 3\u2019 v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer\u2019s protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Partial Ablation", "GSM8403316", "GSM8403316: Liver MTZ 0dppa; Danio rerio; RNA Seq", "GSM8403316 r1", "GSM8403316", "1", "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer's instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell three prime v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer's protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 550", null, "SRP520597", null, "loader:fastq load.py", "ULB-CB-cr-zf-EES1-reload-EES2-0dppa-GEM-240122_S17_L002_I1_001.fastq.gz ULB-CB-cr-zf-EES1-reload-EES2-0dppa-GEM-240122_S17_L002_I2_001.fastq.gz ULB-CB-cr-zf-EES1-reload-EES2-0dppa-GEM-240122_S17_L002_R1_001.fastq.gz ULB-CB-cr-zf-EES1-reload-EES2-0dppa-GEM-240122_S17_L002_R2_001.fastq.gz", "fastq fastq fastq fastq", 22210988016.0, 132208262.0, "GSM8403316 r2", "0:10 1:10 2:28 3:120", "A:4554555932;C:3523295618;G:3687384727;T:4099433293;N:321870", 10, 10, 28, 120, 4554555932, 3523295618, 3687384727, 4099433293, 321870, "SRX25365552", "SRS22031293", "SRA1926595", "Regeneration and Stress Biology, IRIBHM, ULB", "Regeneration and Stress Biology, IRIBHM, ULB", 1, 0.93515, null, 0.07425, null, 0.81251, null, 0.56065, null, 120, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "Belgium", "2024-07-17", "Undetermined", "Larval", "Liver", "Liver and Biliary System"], [33240, "SRR29868314", "SRX25365552", "SRS22031293", "SRP520597", "PRJNA1136957", "Cholangiocytes contribute to hepatocyte regeneration post partial liver injury in larval zebrafish", "GSE272484", "Transcriptome Analysis", "Liver possesses robust regenerative ability  characterized by flexibility in the cellular source of regeneration based on the extent of the injury. post partial hepatectomy or minor injuries  hepatocytes  the primary liver cells  undergo self duplication to replenish the liver mass. In contrast  when the damage is extensive  or hepatocyte proliferation is impaired  cholangiocytes contribute to hepatocyte recovery. This current paradigm of regenerative flexibility in the liver has been established for animals with little or no growth. However  the regenerative mechanisms during periods of growth in young animals remain unexplored. Here  we establish two new partial liver injury protocols in the zebrafish model of rapid growth during late larval stage and observe emergence of de novo hepatocytes in the presence of spared hepatocytes. Using single cell RNA sequencing and lineage tracing  we identify cholangiocytes as the source of de novo hepatocytes. Our study offers a new perspective on the current paradigm of liver regenerating by proposing cholangiocyte to hepatocyte transdifferentiation as the default mechanism of hepatocyte recovery in late larval stage zebrafish. Overall design: We conducted single cell RNA Sequencing using the 10x platform of larval zebrafish liver. This was performed in the basal state Control1  Control2  post partial ablation MTZ 0dppa  MTZ 1dppa and MTZ 9dppa and post partial hepatectomy PHx 4dpi and PHx 11dpi. The two control samples were not treated with 4 OHT  and were also not injured. The control samples are from 13 dpf zebrafish raised under normal rearing condition. For all experiments Tgfabp10a:CellCousin line was used.", null, "pubmed:40480975", null, "Liver MTZ 0dppa", "GSM8403316", null, "source name:Liver|tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Partial Ablation|geo loc name:missing|collection date:missing", "Liver MTZ 0dppa", "A custom Cell Ranger index was generated from the Ensembl GRCz11 genome sequence and annotation filtered with \u2018mkgtf\u2019 command of Cell Ranger options: \u2018\u2014attribute = gene biotype:lincRNA \u2013attribute = gene biotype:antisense  as well as the pseudo mTagBFP2  H2B mGL  mCherry NTR sequences in these cells using the mkref command. This index was used to map reads and generate gene expression matrices using the \u2018count\u2019 command of the Cell Ranger software v.7.1.0 provided by 10x Genomics with the option \u201c\u2013include  introns\u201d set to False all other options were used as per default. Assembly: GRCz11 Supplementary files format and content: Count Matrix as h5ad file Supplementary files format and content: Metadata listing the cell annotations as csv file", "Liver", null, "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer\u2019s instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell 3\u2019 v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer\u2019s protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Partial Ablation", "GSM8403316", "GSM8403316: Liver MTZ 0dppa; Danio rerio; RNA Seq", "GSM8403316 r1", "GSM8403316", "1", "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer's instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell three prime v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer's protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 550", null, "SRP520597", null, "loader:fastq load.py", "ULB-CB-cr-zf-EES1-reload-EES2-0dppa-GEM-240122_S17_L003_I1_001.fastq.gz ULB-CB-cr-zf-EES1-reload-EES2-0dppa-GEM-240122_S17_L003_I2_001.fastq.gz ULB-CB-cr-zf-EES1-reload-EES2-0dppa-GEM-240122_S17_L003_R1_001.fastq.gz ULB-CB-cr-zf-EES1-reload-EES2-0dppa-GEM-240122_S17_L003_R2_001.fastq.gz", "fastq fastq fastq fastq", 22964241384.0, 136691913.0, "GSM8403316 r3", "0:10 1:10 2:28 3:120", "A:4705118723;C:3647298654;G:3815152922;T:4235059775;N:399486", 10, 10, 28, 120, 4705118723, 3647298654, 3815152922, 4235059775, 399486, "SRX25365552", "SRS22031293", "SRA1926595", "Regeneration and Stress Biology, IRIBHM, ULB", "Regeneration and Stress Biology, IRIBHM, ULB", 1, 0.93515, null, 0.07425, null, 0.81085, null, 0.58414, null, 120, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "Belgium", "2024-07-17", "Undetermined", "Larval", "Liver", "Liver and Biliary System"], [33241, "SRR29868315", "SRX25365552", "SRS22031293", "SRP520597", "PRJNA1136957", "Cholangiocytes contribute to hepatocyte regeneration post partial liver injury in larval zebrafish", "GSE272484", "Transcriptome Analysis", "Liver possesses robust regenerative ability  characterized by flexibility in the cellular source of regeneration based on the extent of the injury. post partial hepatectomy or minor injuries  hepatocytes  the primary liver cells  undergo self duplication to replenish the liver mass. In contrast  when the damage is extensive  or hepatocyte proliferation is impaired  cholangiocytes contribute to hepatocyte recovery. This current paradigm of regenerative flexibility in the liver has been established for animals with little or no growth. However  the regenerative mechanisms during periods of growth in young animals remain unexplored. Here  we establish two new partial liver injury protocols in the zebrafish model of rapid growth during late larval stage and observe emergence of de novo hepatocytes in the presence of spared hepatocytes. Using single cell RNA sequencing and lineage tracing  we identify cholangiocytes as the source of de novo hepatocytes. Our study offers a new perspective on the current paradigm of liver regenerating by proposing cholangiocyte to hepatocyte transdifferentiation as the default mechanism of hepatocyte recovery in late larval stage zebrafish. Overall design: We conducted single cell RNA Sequencing using the 10x platform of larval zebrafish liver. This was performed in the basal state Control1  Control2  post partial ablation MTZ 0dppa  MTZ 1dppa and MTZ 9dppa and post partial hepatectomy PHx 4dpi and PHx 11dpi. The two control samples were not treated with 4 OHT  and were also not injured. The control samples are from 13 dpf zebrafish raised under normal rearing condition. For all experiments Tgfabp10a:CellCousin line was used.", null, "pubmed:40480975", null, "Liver MTZ 0dppa", "GSM8403316", null, "source name:Liver|tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Partial Ablation|geo loc name:missing|collection date:missing", "Liver MTZ 0dppa", "A custom Cell Ranger index was generated from the Ensembl GRCz11 genome sequence and annotation filtered with \u2018mkgtf\u2019 command of Cell Ranger options: \u2018\u2014attribute = gene biotype:lincRNA \u2013attribute = gene biotype:antisense  as well as the pseudo mTagBFP2  H2B mGL  mCherry NTR sequences in these cells using the mkref command. This index was used to map reads and generate gene expression matrices using the \u2018count\u2019 command of the Cell Ranger software v.7.1.0 provided by 10x Genomics with the option \u201c\u2013include  introns\u201d set to False all other options were used as per default. Assembly: GRCz11 Supplementary files format and content: Count Matrix as h5ad file Supplementary files format and content: Metadata listing the cell annotations as csv file", "Liver", null, "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer\u2019s instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell 3\u2019 v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer\u2019s protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Partial Ablation", "GSM8403316", "GSM8403316: Liver MTZ 0dppa; Danio rerio; RNA Seq", "GSM8403316 r1", "GSM8403316", "1", "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer's instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell three prime v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer's protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 550", null, "SRP520597", null, "loader:fastq load.py", "ULB-CB-cr-zf-EES1-reload-EES2-0dppa-GEM-240122_S17_L004_I1_001.fastq.gz ULB-CB-cr-zf-EES1-reload-EES2-0dppa-GEM-240122_S17_L004_I2_001.fastq.gz ULB-CB-cr-zf-EES1-reload-EES2-0dppa-GEM-240122_S17_L004_R1_001.fastq.gz ULB-CB-cr-zf-EES1-reload-EES2-0dppa-GEM-240122_S17_L004_R2_001.fastq.gz", "fastq fastq fastq fastq", 22572116784.0, 134357838.0, "GSM8403316 r4", "0:10 1:10 2:28 3:120", "A:4628982434;C:3582861623;G:3746732678;T:4163813221;N:550604", 10, 10, 28, 120, 4628982434, 3582861623, 3746732678, 4163813221, 550604, "SRX25365552", "SRS22031293", "SRA1926595", "Regeneration and Stress Biology, IRIBHM, ULB", "Regeneration and Stress Biology, IRIBHM, ULB", 1, 0.93487, null, 0.07514, null, 0.80862, null, 0.57745, null, 120, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "Belgium", "2024-07-17", "Undetermined", "Larval", "Liver", "Liver and Biliary System"], [33242, "SRR29868316", "SRX25365552", "SRS22031293", "SRP520597", "PRJNA1136957", "Cholangiocytes contribute to hepatocyte regeneration post partial liver injury in larval zebrafish", "GSE272484", "Transcriptome Analysis", "Liver possesses robust regenerative ability  characterized by flexibility in the cellular source of regeneration based on the extent of the injury. post partial hepatectomy or minor injuries  hepatocytes  the primary liver cells  undergo self duplication to replenish the liver mass. In contrast  when the damage is extensive  or hepatocyte proliferation is impaired  cholangiocytes contribute to hepatocyte recovery. This current paradigm of regenerative flexibility in the liver has been established for animals with little or no growth. However  the regenerative mechanisms during periods of growth in young animals remain unexplored. Here  we establish two new partial liver injury protocols in the zebrafish model of rapid growth during late larval stage and observe emergence of de novo hepatocytes in the presence of spared hepatocytes. Using single cell RNA sequencing and lineage tracing  we identify cholangiocytes as the source of de novo hepatocytes. Our study offers a new perspective on the current paradigm of liver regenerating by proposing cholangiocyte to hepatocyte transdifferentiation as the default mechanism of hepatocyte recovery in late larval stage zebrafish. Overall design: We conducted single cell RNA Sequencing using the 10x platform of larval zebrafish liver. This was performed in the basal state Control1  Control2  post partial ablation MTZ 0dppa  MTZ 1dppa and MTZ 9dppa and post partial hepatectomy PHx 4dpi and PHx 11dpi. The two control samples were not treated with 4 OHT  and were also not injured. The control samples are from 13 dpf zebrafish raised under normal rearing condition. For all experiments Tgfabp10a:CellCousin line was used.", null, "pubmed:40480975", null, "Liver MTZ 0dppa", "GSM8403316", null, "source name:Liver|tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Partial Ablation|geo loc name:missing|collection date:missing", "Liver MTZ 0dppa", "A custom Cell Ranger index was generated from the Ensembl GRCz11 genome sequence and annotation filtered with \u2018mkgtf\u2019 command of Cell Ranger options: \u2018\u2014attribute = gene biotype:lincRNA \u2013attribute = gene biotype:antisense  as well as the pseudo mTagBFP2  H2B mGL  mCherry NTR sequences in these cells using the mkref command. This index was used to map reads and generate gene expression matrices using the \u2018count\u2019 command of the Cell Ranger software v.7.1.0 provided by 10x Genomics with the option \u201c\u2013include  introns\u201d set to False all other options were used as per default. Assembly: GRCz11 Supplementary files format and content: Count Matrix as h5ad file Supplementary files format and content: Metadata listing the cell annotations as csv file", "Liver", null, "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer\u2019s instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell 3\u2019 v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer\u2019s protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Partial Ablation", "GSM8403316", "GSM8403316: Liver MTZ 0dppa; Danio rerio; RNA Seq", "GSM8403316 r1", "GSM8403316", "1", "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer's instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell three prime v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer's protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 550", null, "SRP520597", null, "loader:fastq load.py", "ULB-CB-cr-zf-EES2-0dppa-GEM-240122_S34_L001_I1_001.fastq.gz ULB-CB-cr-zf-EES2-0dppa-GEM-240122_S34_L001_I2_001.fastq.gz ULB-CB-cr-zf-EES2-0dppa-GEM-240122_S34_L001_R1_001.fastq.gz ULB-CB-cr-zf-EES2-0dppa-GEM-240122_S34_L001_R2_001.fastq.gz", "fastq fastq fastq fastq", 9319118424.0, 55470943.0, "GSM8403316 r5", "0:10 1:10 2:28 3:120", "A:1895887547;C:1477863924;G:1543846946;T:1738857665;N:57078", 10, 10, 28, 120, 1895887547, 1477863924, 1543846946, 1738857665, 57078, "SRX25365552", "SRS22031293", "SRA1926595", "Regeneration and Stress Biology, IRIBHM, ULB", "Regeneration and Stress Biology, IRIBHM, ULB", 1, 0.93507, null, 0.07612, null, 0.80862, null, 0.5708, null, 120, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "Belgium", "2024-07-17", "Undetermined", "Larval", "Liver", "Liver and Biliary System"], [33243, "SRR29868317", "SRX25365552", "SRS22031293", "SRP520597", "PRJNA1136957", "Cholangiocytes contribute to hepatocyte regeneration post partial liver injury in larval zebrafish", "GSE272484", "Transcriptome Analysis", "Liver possesses robust regenerative ability  characterized by flexibility in the cellular source of regeneration based on the extent of the injury. post partial hepatectomy or minor injuries  hepatocytes  the primary liver cells  undergo self duplication to replenish the liver mass. In contrast  when the damage is extensive  or hepatocyte proliferation is impaired  cholangiocytes contribute to hepatocyte recovery. This current paradigm of regenerative flexibility in the liver has been established for animals with little or no growth. However  the regenerative mechanisms during periods of growth in young animals remain unexplored. Here  we establish two new partial liver injury protocols in the zebrafish model of rapid growth during late larval stage and observe emergence of de novo hepatocytes in the presence of spared hepatocytes. Using single cell RNA sequencing and lineage tracing  we identify cholangiocytes as the source of de novo hepatocytes. Our study offers a new perspective on the current paradigm of liver regenerating by proposing cholangiocyte to hepatocyte transdifferentiation as the default mechanism of hepatocyte recovery in late larval stage zebrafish. Overall design: We conducted single cell RNA Sequencing using the 10x platform of larval zebrafish liver. This was performed in the basal state Control1  Control2  post partial ablation MTZ 0dppa  MTZ 1dppa and MTZ 9dppa and post partial hepatectomy PHx 4dpi and PHx 11dpi. The two control samples were not treated with 4 OHT  and were also not injured. The control samples are from 13 dpf zebrafish raised under normal rearing condition. For all experiments Tgfabp10a:CellCousin line was used.", null, "pubmed:40480975", null, "Liver MTZ 0dppa", "GSM8403316", null, "source name:Liver|tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Partial Ablation|geo loc name:missing|collection date:missing", "Liver MTZ 0dppa", "A custom Cell Ranger index was generated from the Ensembl GRCz11 genome sequence and annotation filtered with \u2018mkgtf\u2019 command of Cell Ranger options: \u2018\u2014attribute = gene biotype:lincRNA \u2013attribute = gene biotype:antisense  as well as the pseudo mTagBFP2  H2B mGL  mCherry NTR sequences in these cells using the mkref command. This index was used to map reads and generate gene expression matrices using the \u2018count\u2019 command of the Cell Ranger software v.7.1.0 provided by 10x Genomics with the option \u201c\u2013include  introns\u201d set to False all other options were used as per default. Assembly: GRCz11 Supplementary files format and content: Count Matrix as h5ad file Supplementary files format and content: Metadata listing the cell annotations as csv file", "Liver", null, "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer\u2019s instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell 3\u2019 v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer\u2019s protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Partial Ablation", "GSM8403316", "GSM8403316: Liver MTZ 0dppa; Danio rerio; RNA Seq", "GSM8403316 r1", "GSM8403316", "1", "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer's instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell three prime v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer's protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 550", null, "SRP520597", null, "loader:fastq load.py", "ULB-CB-cr-zf-EES2-0dppa-GEM-240122_S34_L002_I1_001.fastq.gz ULB-CB-cr-zf-EES2-0dppa-GEM-240122_S34_L002_I2_001.fastq.gz ULB-CB-cr-zf-EES2-0dppa-GEM-240122_S34_L002_R1_001.fastq.gz ULB-CB-cr-zf-EES2-0dppa-GEM-240122_S34_L002_R2_001.fastq.gz", "fastq fastq fastq fastq", 9433874184.0, 56154013.0, "GSM8403316 r6", "0:10 1:10 2:28 3:120", "A:1919903956;C:1496318299;G:1560772790;T:1761443349;N:43166", 10, 10, 28, 120, 1919903956, 1496318299, 1560772790, 1761443349, 43166, "SRX25365552", "SRS22031293", "SRA1926595", "Regeneration and Stress Biology, IRIBHM, ULB", "Regeneration and Stress Biology, IRIBHM, ULB", 1, 0.93514, null, 0.07597, null, 0.80937, null, 0.5877, null, 120, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "Belgium", "2024-07-17", "Undetermined", "Larval", "Liver", "Liver and Biliary System"], [33244, "SRR29868318", "SRX25365552", "SRS22031293", "SRP520597", "PRJNA1136957", "Cholangiocytes contribute to hepatocyte regeneration post partial liver injury in larval zebrafish", "GSE272484", "Transcriptome Analysis", "Liver possesses robust regenerative ability  characterized by flexibility in the cellular source of regeneration based on the extent of the injury. post partial hepatectomy or minor injuries  hepatocytes  the primary liver cells  undergo self duplication to replenish the liver mass. In contrast  when the damage is extensive  or hepatocyte proliferation is impaired  cholangiocytes contribute to hepatocyte recovery. This current paradigm of regenerative flexibility in the liver has been established for animals with little or no growth. However  the regenerative mechanisms during periods of growth in young animals remain unexplored. Here  we establish two new partial liver injury protocols in the zebrafish model of rapid growth during late larval stage and observe emergence of de novo hepatocytes in the presence of spared hepatocytes. Using single cell RNA sequencing and lineage tracing  we identify cholangiocytes as the source of de novo hepatocytes. Our study offers a new perspective on the current paradigm of liver regenerating by proposing cholangiocyte to hepatocyte transdifferentiation as the default mechanism of hepatocyte recovery in late larval stage zebrafish. Overall design: We conducted single cell RNA Sequencing using the 10x platform of larval zebrafish liver. This was performed in the basal state Control1  Control2  post partial ablation MTZ 0dppa  MTZ 1dppa and MTZ 9dppa and post partial hepatectomy PHx 4dpi and PHx 11dpi. The two control samples were not treated with 4 OHT  and were also not injured. The control samples are from 13 dpf zebrafish raised under normal rearing condition. For all experiments Tgfabp10a:CellCousin line was used.", null, "pubmed:40480975", null, "Liver MTZ 0dppa", "GSM8403316", null, "source name:Liver|tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Partial Ablation|geo loc name:missing|collection date:missing", "Liver MTZ 0dppa", "A custom Cell Ranger index was generated from the Ensembl GRCz11 genome sequence and annotation filtered with \u2018mkgtf\u2019 command of Cell Ranger options: \u2018\u2014attribute = gene biotype:lincRNA \u2013attribute = gene biotype:antisense  as well as the pseudo mTagBFP2  H2B mGL  mCherry NTR sequences in these cells using the mkref command. This index was used to map reads and generate gene expression matrices using the \u2018count\u2019 command of the Cell Ranger software v.7.1.0 provided by 10x Genomics with the option \u201c\u2013include  introns\u201d set to False all other options were used as per default. Assembly: GRCz11 Supplementary files format and content: Count Matrix as h5ad file Supplementary files format and content: Metadata listing the cell annotations as csv file", "Liver", null, "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer\u2019s instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell 3\u2019 v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer\u2019s protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Partial Ablation", "GSM8403316", "GSM8403316: Liver MTZ 0dppa; Danio rerio; RNA Seq", "GSM8403316 r1", "GSM8403316", "1", "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer's instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell three prime v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer's protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 550", null, "SRP520597", null, "loader:fastq load.py", "ULB-CB-cr-zf-EES2-0dppa-GEM-240122_S34_L003_R2_001.fastq.gz ULB-CB-cr-zf-EES2-0dppa-GEM-240122_S34_L003_R1_001.fastq.gz ULB-CB-cr-zf-EES2-0dppa-GEM-240122_S34_L003_I2_001.fastq.gz ULB-CB-cr-zf-EES2-0dppa-GEM-240122_S34_L003_I1_001.fastq.gz", "fastq fastq fastq fastq", 9674650944.0, 57587208.0, "GSM8403316 r7", "0:10 1:10 2:28 3:120", "A:1969538339;C:1535292977;G:1601577456;T:1804003343;N:52845", 10, 10, 28, 120, 1969538339, 1535292977, 1601577456, 1804003343, 52845, "SRX25365552", "SRS22031293", "SRA1926595", "Regeneration and Stress Biology, IRIBHM, ULB", "Regeneration and Stress Biology, IRIBHM, ULB", 1, 0.93521, null, 0.07665, null, 0.80876, null, 0.57512, null, 120, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "Belgium", "2024-07-17", "Undetermined", "Larval", "Liver", "Liver and Biliary System"], [33245, "SRR29868319", "SRX25365552", "SRS22031293", "SRP520597", "PRJNA1136957", "Cholangiocytes contribute to hepatocyte regeneration post partial liver injury in larval zebrafish", "GSE272484", "Transcriptome Analysis", "Liver possesses robust regenerative ability  characterized by flexibility in the cellular source of regeneration based on the extent of the injury. post partial hepatectomy or minor injuries  hepatocytes  the primary liver cells  undergo self duplication to replenish the liver mass. In contrast  when the damage is extensive  or hepatocyte proliferation is impaired  cholangiocytes contribute to hepatocyte recovery. This current paradigm of regenerative flexibility in the liver has been established for animals with little or no growth. However  the regenerative mechanisms during periods of growth in young animals remain unexplored. Here  we establish two new partial liver injury protocols in the zebrafish model of rapid growth during late larval stage and observe emergence of de novo hepatocytes in the presence of spared hepatocytes. Using single cell RNA sequencing and lineage tracing  we identify cholangiocytes as the source of de novo hepatocytes. Our study offers a new perspective on the current paradigm of liver regenerating by proposing cholangiocyte to hepatocyte transdifferentiation as the default mechanism of hepatocyte recovery in late larval stage zebrafish. Overall design: We conducted single cell RNA Sequencing using the 10x platform of larval zebrafish liver. This was performed in the basal state Control1  Control2  post partial ablation MTZ 0dppa  MTZ 1dppa and MTZ 9dppa and post partial hepatectomy PHx 4dpi and PHx 11dpi. The two control samples were not treated with 4 OHT  and were also not injured. The control samples are from 13 dpf zebrafish raised under normal rearing condition. For all experiments Tgfabp10a:CellCousin line was used.", null, "pubmed:40480975", null, "Liver MTZ 0dppa", "GSM8403316", null, "source name:Liver|tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Partial Ablation|geo loc name:missing|collection date:missing", "Liver MTZ 0dppa", "A custom Cell Ranger index was generated from the Ensembl GRCz11 genome sequence and annotation filtered with \u2018mkgtf\u2019 command of Cell Ranger options: \u2018\u2014attribute = gene biotype:lincRNA \u2013attribute = gene biotype:antisense  as well as the pseudo mTagBFP2  H2B mGL  mCherry NTR sequences in these cells using the mkref command. This index was used to map reads and generate gene expression matrices using the \u2018count\u2019 command of the Cell Ranger software v.7.1.0 provided by 10x Genomics with the option \u201c\u2013include  introns\u201d set to False all other options were used as per default. Assembly: GRCz11 Supplementary files format and content: Count Matrix as h5ad file Supplementary files format and content: Metadata listing the cell annotations as csv file", "Liver", null, "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer\u2019s instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell 3\u2019 v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer\u2019s protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Partial Ablation", "GSM8403316", "GSM8403316: Liver MTZ 0dppa; Danio rerio; RNA Seq", "GSM8403316 r1", "GSM8403316", "1", "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer's instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell three prime v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer's protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 550", null, "SRP520597", null, "loader:fastq load.py", "ULB-CB-cr-zf-EES2-0dppa-GEM-240122_S34_L004_I1_001.fastq.gz ULB-CB-cr-zf-EES2-0dppa-GEM-240122_S34_L004_I2_001.fastq.gz ULB-CB-cr-zf-EES2-0dppa-GEM-240122_S34_L004_R1_001.fastq.gz ULB-CB-cr-zf-EES2-0dppa-GEM-240122_S34_L004_R2_001.fastq.gz", "fastq fastq fastq fastq", 9063172776.0, 53947457.0, "GSM8403316 r8", "0:10 1:10 2:28 3:120", "A:1844279220;C:1435218712;G:1495137465;T:1699009389;N:50054", 10, 10, 28, 120, 1844279220, 1435218712, 1495137465, 1699009389, 50054, "SRX25365552", "SRS22031293", "SRA1926595", "Regeneration and Stress Biology, IRIBHM, ULB", "Regeneration and Stress Biology, IRIBHM, ULB", 1, 0.93431, null, 0.07653, null, 0.80803, null, 0.57631, null, 120, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "Belgium", "2024-07-17", "Undetermined", "Larval", "Liver", "Liver and Biliary System"], [33246, "SRR29868323", "SRX25365551", "SRS22031292", "SRP520597", "PRJNA1136957", "Cholangiocytes contribute to hepatocyte regeneration post partial liver injury in larval zebrafish", "GSE272484", "Transcriptome Analysis", "Liver possesses robust regenerative ability  characterized by flexibility in the cellular source of regeneration based on the extent of the injury. post partial hepatectomy or minor injuries  hepatocytes  the primary liver cells  undergo self duplication to replenish the liver mass. In contrast  when the damage is extensive  or hepatocyte proliferation is impaired  cholangiocytes contribute to hepatocyte recovery. This current paradigm of regenerative flexibility in the liver has been established for animals with little or no growth. However  the regenerative mechanisms during periods of growth in young animals remain unexplored. Here  we establish two new partial liver injury protocols in the zebrafish model of rapid growth during late larval stage and observe emergence of de novo hepatocytes in the presence of spared hepatocytes. Using single cell RNA sequencing and lineage tracing  we identify cholangiocytes as the source of de novo hepatocytes. Our study offers a new perspective on the current paradigm of liver regenerating by proposing cholangiocyte to hepatocyte transdifferentiation as the default mechanism of hepatocyte recovery in late larval stage zebrafish. Overall design: We conducted single cell RNA Sequencing using the 10x platform of larval zebrafish liver. This was performed in the basal state Control1  Control2  post partial ablation MTZ 0dppa  MTZ 1dppa and MTZ 9dppa and post partial hepatectomy PHx 4dpi and PHx 11dpi. The two control samples were not treated with 4 OHT  and were also not injured. The control samples are from 13 dpf zebrafish raised under normal rearing condition. For all experiments Tgfabp10a:CellCousin line was used.", null, "pubmed:40480975", null, "Liver Control1", "GSM8403314", null, "source name:Liver|tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Control \u2013 No 4 OHT|geo loc name:missing|collection date:missing", "Liver Control1", "A custom Cell Ranger index was generated from the Ensembl GRCz11 genome sequence and annotation filtered with \u2018mkgtf\u2019 command of Cell Ranger options: \u2018\u2014attribute = gene biotype:lincRNA \u2013attribute = gene biotype:antisense  as well as the pseudo mTagBFP2  H2B mGL  mCherry NTR sequences in these cells using the mkref command. This index was used to map reads and generate gene expression matrices using the \u2018count\u2019 command of the Cell Ranger software v.7.1.0 provided by 10x Genomics with the option \u201c\u2013include  introns\u201d set to False all other options were used as per default. Assembly: GRCz11 Supplementary files format and content: Count Matrix as h5ad file Supplementary files format and content: Metadata listing the cell annotations as csv file", "Liver", null, "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer\u2019s instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell 3\u2019 v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer\u2019s protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Control \u2013 No 4 OHT", "GSM8403314", "GSM8403314: Liver Control1; Danio rerio; RNA Seq", "GSM8403314 r1", "GSM8403314", "1", "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer's instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell three prime v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer's protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 550", null, "SRP520597", null, "loader:fastq load.py", "ULB-CB-cr-zf-EES1-larvalliver-CTL113dpf-GEM-230727_S10_L001_R2_001.fastq.gz ULB-CB-cr-zf-EES1-larvalliver-CTL113dpf-GEM-230727_S10_L001_R1_001.fastq.gz ULB-CB-cr-zf-EES1-larvalliver-CTL113dpf-GEM-230727_S10_L001_I2_001.fastq.gz ULB-CB-cr-zf-EES1-larvalliver-CTL113dpf-GEM-230727_S10_L001_I1_001.fastq.gz", "fastq fastq fastq fastq", 10244575656.0, 60979617.0, "GSM8403314 r1", "0:10 1:10 2:28 3:120", "A:2119295536;C:1665865582;G:1726802243;T:1805507036;N:83643", 10, 10, 28, 120, 2119295536, 1665865582, 1726802243, 1805507036, 83643, "SRX25365551", "SRS22031292", "SRA1926595", "Regeneration and Stress Biology, IRIBHM, ULB", "Regeneration and Stress Biology, IRIBHM, ULB", 1, 0.92821, null, 0.09448, null, 0.83725, null, 0.60089, null, 120, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "Belgium", "2024-07-17", "Undetermined", "Larval", "Liver", "Liver and Biliary System"], [33247, "SRR29868324", "SRX25365551", "SRS22031292", "SRP520597", "PRJNA1136957", "Cholangiocytes contribute to hepatocyte regeneration post partial liver injury in larval zebrafish", "GSE272484", "Transcriptome Analysis", "Liver possesses robust regenerative ability  characterized by flexibility in the cellular source of regeneration based on the extent of the injury. post partial hepatectomy or minor injuries  hepatocytes  the primary liver cells  undergo self duplication to replenish the liver mass. In contrast  when the damage is extensive  or hepatocyte proliferation is impaired  cholangiocytes contribute to hepatocyte recovery. This current paradigm of regenerative flexibility in the liver has been established for animals with little or no growth. However  the regenerative mechanisms during periods of growth in young animals remain unexplored. Here  we establish two new partial liver injury protocols in the zebrafish model of rapid growth during late larval stage and observe emergence of de novo hepatocytes in the presence of spared hepatocytes. Using single cell RNA sequencing and lineage tracing  we identify cholangiocytes as the source of de novo hepatocytes. Our study offers a new perspective on the current paradigm of liver regenerating by proposing cholangiocyte to hepatocyte transdifferentiation as the default mechanism of hepatocyte recovery in late larval stage zebrafish. Overall design: We conducted single cell RNA Sequencing using the 10x platform of larval zebrafish liver. This was performed in the basal state Control1  Control2  post partial ablation MTZ 0dppa  MTZ 1dppa and MTZ 9dppa and post partial hepatectomy PHx 4dpi and PHx 11dpi. The two control samples were not treated with 4 OHT  and were also not injured. The control samples are from 13 dpf zebrafish raised under normal rearing condition. For all experiments Tgfabp10a:CellCousin line was used.", null, "pubmed:40480975", null, "Liver Control1", "GSM8403314", null, "source name:Liver|tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Control \u2013 No 4 OHT|geo loc name:missing|collection date:missing", "Liver Control1", "A custom Cell Ranger index was generated from the Ensembl GRCz11 genome sequence and annotation filtered with \u2018mkgtf\u2019 command of Cell Ranger options: \u2018\u2014attribute = gene biotype:lincRNA \u2013attribute = gene biotype:antisense  as well as the pseudo mTagBFP2  H2B mGL  mCherry NTR sequences in these cells using the mkref command. This index was used to map reads and generate gene expression matrices using the \u2018count\u2019 command of the Cell Ranger software v.7.1.0 provided by 10x Genomics with the option \u201c\u2013include  introns\u201d set to False all other options were used as per default. Assembly: GRCz11 Supplementary files format and content: Count Matrix as h5ad file Supplementary files format and content: Metadata listing the cell annotations as csv file", "Liver", null, "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer\u2019s instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell 3\u2019 v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer\u2019s protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Control \u2013 No 4 OHT", "GSM8403314", "GSM8403314: Liver Control1; Danio rerio; RNA Seq", "GSM8403314 r1", "GSM8403314", "1", "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer's instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell three prime v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer's protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 550", null, "SRP520597", null, "loader:fastq load.py", "ULB-CB-cr-zf-EES1-larvalliver-CTL113dpf-GEM-230727_S10_L002_R2_001.fastq.gz ULB-CB-cr-zf-EES1-larvalliver-CTL113dpf-GEM-230727_S10_L002_R1_001.fastq.gz ULB-CB-cr-zf-EES1-larvalliver-CTL113dpf-GEM-230727_S10_L002_I2_001.fastq.gz ULB-CB-cr-zf-EES1-larvalliver-CTL113dpf-GEM-230727_S10_L002_I1_001.fastq.gz", "fastq fastq fastq fastq", 10380032880.0, 61785910.0, "GSM8403314 r2", "0:10 1:10 2:28 3:120", "A:2148125611;C:1687299842;G:1748516982;T:1830286430;N:80335", 10, 10, 28, 120, 2148125611, 1687299842, 1748516982, 1830286430, 80335, "SRX25365551", "SRS22031292", "SRA1926595", "Regeneration and Stress Biology, IRIBHM, ULB", "Regeneration and Stress Biology, IRIBHM, ULB", 1, 0.93012, null, 0.0931, null, 0.83621, null, 0.60089, null, 120, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "Belgium", "2024-07-17", "Undetermined", "Larval", "Liver", "Liver and Biliary System"], [33248, "SRR29868325", "SRX25365551", "SRS22031292", "SRP520597", "PRJNA1136957", "Cholangiocytes contribute to hepatocyte regeneration post partial liver injury in larval zebrafish", "GSE272484", "Transcriptome Analysis", "Liver possesses robust regenerative ability  characterized by flexibility in the cellular source of regeneration based on the extent of the injury. post partial hepatectomy or minor injuries  hepatocytes  the primary liver cells  undergo self duplication to replenish the liver mass. In contrast  when the damage is extensive  or hepatocyte proliferation is impaired  cholangiocytes contribute to hepatocyte recovery. This current paradigm of regenerative flexibility in the liver has been established for animals with little or no growth. However  the regenerative mechanisms during periods of growth in young animals remain unexplored. Here  we establish two new partial liver injury protocols in the zebrafish model of rapid growth during late larval stage and observe emergence of de novo hepatocytes in the presence of spared hepatocytes. Using single cell RNA sequencing and lineage tracing  we identify cholangiocytes as the source of de novo hepatocytes. Our study offers a new perspective on the current paradigm of liver regenerating by proposing cholangiocyte to hepatocyte transdifferentiation as the default mechanism of hepatocyte recovery in late larval stage zebrafish. Overall design: We conducted single cell RNA Sequencing using the 10x platform of larval zebrafish liver. This was performed in the basal state Control1  Control2  post partial ablation MTZ 0dppa  MTZ 1dppa and MTZ 9dppa and post partial hepatectomy PHx 4dpi and PHx 11dpi. The two control samples were not treated with 4 OHT  and were also not injured. The control samples are from 13 dpf zebrafish raised under normal rearing condition. For all experiments Tgfabp10a:CellCousin line was used.", null, "pubmed:40480975", null, "Liver Control1", "GSM8403314", null, "source name:Liver|tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Control \u2013 No 4 OHT|geo loc name:missing|collection date:missing", "Liver Control1", "A custom Cell Ranger index was generated from the Ensembl GRCz11 genome sequence and annotation filtered with \u2018mkgtf\u2019 command of Cell Ranger options: \u2018\u2014attribute = gene biotype:lincRNA \u2013attribute = gene biotype:antisense  as well as the pseudo mTagBFP2  H2B mGL  mCherry NTR sequences in these cells using the mkref command. This index was used to map reads and generate gene expression matrices using the \u2018count\u2019 command of the Cell Ranger software v.7.1.0 provided by 10x Genomics with the option \u201c\u2013include  introns\u201d set to False all other options were used as per default. Assembly: GRCz11 Supplementary files format and content: Count Matrix as h5ad file Supplementary files format and content: Metadata listing the cell annotations as csv file", "Liver", null, "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer\u2019s instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell 3\u2019 v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer\u2019s protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Control \u2013 No 4 OHT", "GSM8403314", "GSM8403314: Liver Control1; Danio rerio; RNA Seq", "GSM8403314 r1", "GSM8403314", "1", "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer's instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell three prime v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer's protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 550", null, "SRP520597", null, "loader:fastq load.py", "ULB-CB-cr-zf-EES1-larvalliver-CTL113dpf-GEM-230727_S10_L003_R2_001.fastq.gz ULB-CB-cr-zf-EES1-larvalliver-CTL113dpf-GEM-230727_S10_L003_R1_001.fastq.gz ULB-CB-cr-zf-EES1-larvalliver-CTL113dpf-GEM-230727_S10_L003_I2_001.fastq.gz ULB-CB-cr-zf-EES1-larvalliver-CTL113dpf-GEM-230727_S10_L003_I1_001.fastq.gz", "fastq fastq fastq fastq", 10397790648.0, 61891611.0, "GSM8403314 r3", "0:10 1:10 2:28 3:120", "A:2150008358;C:1691781234;G:1752867785;T:1832258443;N:77500", 10, 10, 28, 120, 2150008358, 1691781234, 1752867785, 1832258443, 77500, "SRX25365551", "SRS22031292", "SRA1926595", "Regeneration and Stress Biology, IRIBHM, ULB", "Regeneration and Stress Biology, IRIBHM, ULB", 1, 0.9284, null, 0.09293, null, 0.83605, null, 0.59853, null, 120, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "Belgium", "2024-07-17", "Undetermined", "Larval", "Liver", "Liver and Biliary System"], [33249, "SRR29868326", "SRX25365551", "SRS22031292", "SRP520597", "PRJNA1136957", "Cholangiocytes contribute to hepatocyte regeneration post partial liver injury in larval zebrafish", "GSE272484", "Transcriptome Analysis", "Liver possesses robust regenerative ability  characterized by flexibility in the cellular source of regeneration based on the extent of the injury. post partial hepatectomy or minor injuries  hepatocytes  the primary liver cells  undergo self duplication to replenish the liver mass. In contrast  when the damage is extensive  or hepatocyte proliferation is impaired  cholangiocytes contribute to hepatocyte recovery. This current paradigm of regenerative flexibility in the liver has been established for animals with little or no growth. However  the regenerative mechanisms during periods of growth in young animals remain unexplored. Here  we establish two new partial liver injury protocols in the zebrafish model of rapid growth during late larval stage and observe emergence of de novo hepatocytes in the presence of spared hepatocytes. Using single cell RNA sequencing and lineage tracing  we identify cholangiocytes as the source of de novo hepatocytes. Our study offers a new perspective on the current paradigm of liver regenerating by proposing cholangiocyte to hepatocyte transdifferentiation as the default mechanism of hepatocyte recovery in late larval stage zebrafish. Overall design: We conducted single cell RNA Sequencing using the 10x platform of larval zebrafish liver. This was performed in the basal state Control1  Control2  post partial ablation MTZ 0dppa  MTZ 1dppa and MTZ 9dppa and post partial hepatectomy PHx 4dpi and PHx 11dpi. The two control samples were not treated with 4 OHT  and were also not injured. The control samples are from 13 dpf zebrafish raised under normal rearing condition. For all experiments Tgfabp10a:CellCousin line was used.", null, "pubmed:40480975", null, "Liver Control1", "GSM8403314", null, "source name:Liver|tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Control \u2013 No 4 OHT|geo loc name:missing|collection date:missing", "Liver Control1", "A custom Cell Ranger index was generated from the Ensembl GRCz11 genome sequence and annotation filtered with \u2018mkgtf\u2019 command of Cell Ranger options: \u2018\u2014attribute = gene biotype:lincRNA \u2013attribute = gene biotype:antisense  as well as the pseudo mTagBFP2  H2B mGL  mCherry NTR sequences in these cells using the mkref command. This index was used to map reads and generate gene expression matrices using the \u2018count\u2019 command of the Cell Ranger software v.7.1.0 provided by 10x Genomics with the option \u201c\u2013include  introns\u201d set to False all other options were used as per default. Assembly: GRCz11 Supplementary files format and content: Count Matrix as h5ad file Supplementary files format and content: Metadata listing the cell annotations as csv file", "Liver", null, "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer\u2019s instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell 3\u2019 v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer\u2019s protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Control \u2013 No 4 OHT", "GSM8403314", "GSM8403314: Liver Control1; Danio rerio; RNA Seq", "GSM8403314 r1", "GSM8403314", "1", "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer's instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell three prime v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer's protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 550", null, "SRP520597", null, "loader:fastq load.py", "ULB-CB-cr-zf-EES1-larvalliver-CTL113dpf-GEM-230727_S10_L004_R2_001.fastq.gz ULB-CB-cr-zf-EES1-larvalliver-CTL113dpf-GEM-230727_S10_L004_R1_001.fastq.gz ULB-CB-cr-zf-EES1-larvalliver-CTL113dpf-GEM-230727_S10_L004_I2_001.fastq.gz ULB-CB-cr-zf-EES1-larvalliver-CTL113dpf-GEM-230727_S10_L004_I1_001.fastq.gz", "fastq fastq fastq fastq", 10333943592.0, 61511569.0, "GSM8403314 r4", "0:10 1:10 2:28 3:120", "A:2138003833;C:1680536471;G:1740834135;T:1821926938;N:86903", 10, 10, 28, 120, 2138003833, 1680536471, 1740834135, 1821926938, 86903, "SRX25365551", "SRS22031292", "SRA1926595", "Regeneration and Stress Biology, IRIBHM, ULB", "Regeneration and Stress Biology, IRIBHM, ULB", 1, 0.92828, null, 0.09437, null, 0.83611, null, 0.58915, null, 120, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "Belgium", "2024-07-17", "Undetermined", "Larval", "Liver", "Liver and Biliary System"], [33250, "SRR29868327", "SRX25365551", "SRS22031292", "SRP520597", "PRJNA1136957", "Cholangiocytes contribute to hepatocyte regeneration post partial liver injury in larval zebrafish", "GSE272484", "Transcriptome Analysis", "Liver possesses robust regenerative ability  characterized by flexibility in the cellular source of regeneration based on the extent of the injury. post partial hepatectomy or minor injuries  hepatocytes  the primary liver cells  undergo self duplication to replenish the liver mass. In contrast  when the damage is extensive  or hepatocyte proliferation is impaired  cholangiocytes contribute to hepatocyte recovery. This current paradigm of regenerative flexibility in the liver has been established for animals with little or no growth. However  the regenerative mechanisms during periods of growth in young animals remain unexplored. Here  we establish two new partial liver injury protocols in the zebrafish model of rapid growth during late larval stage and observe emergence of de novo hepatocytes in the presence of spared hepatocytes. Using single cell RNA sequencing and lineage tracing  we identify cholangiocytes as the source of de novo hepatocytes. Our study offers a new perspective on the current paradigm of liver regenerating by proposing cholangiocyte to hepatocyte transdifferentiation as the default mechanism of hepatocyte recovery in late larval stage zebrafish. Overall design: We conducted single cell RNA Sequencing using the 10x platform of larval zebrafish liver. This was performed in the basal state Control1  Control2  post partial ablation MTZ 0dppa  MTZ 1dppa and MTZ 9dppa and post partial hepatectomy PHx 4dpi and PHx 11dpi. The two control samples were not treated with 4 OHT  and were also not injured. The control samples are from 13 dpf zebrafish raised under normal rearing condition. For all experiments Tgfabp10a:CellCousin line was used.", null, "pubmed:40480975", null, "Liver Control1", "GSM8403314", null, "source name:Liver|tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Control \u2013 No 4 OHT|geo loc name:missing|collection date:missing", "Liver Control1", "A custom Cell Ranger index was generated from the Ensembl GRCz11 genome sequence and annotation filtered with \u2018mkgtf\u2019 command of Cell Ranger options: \u2018\u2014attribute = gene biotype:lincRNA \u2013attribute = gene biotype:antisense  as well as the pseudo mTagBFP2  H2B mGL  mCherry NTR sequences in these cells using the mkref command. This index was used to map reads and generate gene expression matrices using the \u2018count\u2019 command of the Cell Ranger software v.7.1.0 provided by 10x Genomics with the option \u201c\u2013include  introns\u201d set to False all other options were used as per default. Assembly: GRCz11 Supplementary files format and content: Count Matrix as h5ad file Supplementary files format and content: Metadata listing the cell annotations as csv file", "Liver", null, "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer\u2019s instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell 3\u2019 v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer\u2019s protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Control \u2013 No 4 OHT", "GSM8403314", "GSM8403314: Liver Control1; Danio rerio; RNA Seq", "GSM8403314 r1", "GSM8403314", "1", "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer's instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell three prime v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer's protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 550", null, "SRP520597", null, "loader:fastq load.py", "ULB-CB-cr-zf-EES3-reload-EES1-larvlivCTL113dpf-GEM-230727_S19_L001_I1_001.fastq.gz ULB-CB-cr-zf-EES3-reload-EES1-larvlivCTL113dpf-GEM-230727_S19_L001_R2_001.fastq.gz ULB-CB-cr-zf-EES3-reload-EES1-larvlivCTL113dpf-GEM-230727_S19_L001_R1_001.fastq.gz ULB-CB-cr-zf-EES3-reload-EES1-larvlivCTL113dpf-GEM-230727_S19_L001_I2_001.fastq.gz", "fastq fastq fastq fastq", 11552239440.0, 68763330.0, "GSM8403314 r5", "0:10 1:10 2:28 3:120", "A:2384461898;C:1875086137;G:1950680109;T:2041249149;N:122307", 10, 10, 28, 120, 2384461898, 1875086137, 1950680109, 2041249149, 122307, "SRX25365551", "SRS22031292", "SRA1926595", "Regeneration and Stress Biology, IRIBHM, ULB", "Regeneration and Stress Biology, IRIBHM, ULB", 1, 0.92842, null, 0.09581, null, 0.83591, null, 0.59709, null, 120, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "Belgium", "2024-07-17", "Undetermined", "Larval", "Liver", "Liver and Biliary System"], [33251, "SRR29868328", "SRX25365551", "SRS22031292", "SRP520597", "PRJNA1136957", "Cholangiocytes contribute to hepatocyte regeneration post partial liver injury in larval zebrafish", "GSE272484", "Transcriptome Analysis", "Liver possesses robust regenerative ability  characterized by flexibility in the cellular source of regeneration based on the extent of the injury. post partial hepatectomy or minor injuries  hepatocytes  the primary liver cells  undergo self duplication to replenish the liver mass. In contrast  when the damage is extensive  or hepatocyte proliferation is impaired  cholangiocytes contribute to hepatocyte recovery. This current paradigm of regenerative flexibility in the liver has been established for animals with little or no growth. However  the regenerative mechanisms during periods of growth in young animals remain unexplored. Here  we establish two new partial liver injury protocols in the zebrafish model of rapid growth during late larval stage and observe emergence of de novo hepatocytes in the presence of spared hepatocytes. Using single cell RNA sequencing and lineage tracing  we identify cholangiocytes as the source of de novo hepatocytes. Our study offers a new perspective on the current paradigm of liver regenerating by proposing cholangiocyte to hepatocyte transdifferentiation as the default mechanism of hepatocyte recovery in late larval stage zebrafish. Overall design: We conducted single cell RNA Sequencing using the 10x platform of larval zebrafish liver. This was performed in the basal state Control1  Control2  post partial ablation MTZ 0dppa  MTZ 1dppa and MTZ 9dppa and post partial hepatectomy PHx 4dpi and PHx 11dpi. The two control samples were not treated with 4 OHT  and were also not injured. The control samples are from 13 dpf zebrafish raised under normal rearing condition. For all experiments Tgfabp10a:CellCousin line was used.", null, "pubmed:40480975", null, "Liver Control1", "GSM8403314", null, "source name:Liver|tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Control \u2013 No 4 OHT|geo loc name:missing|collection date:missing", "Liver Control1", "A custom Cell Ranger index was generated from the Ensembl GRCz11 genome sequence and annotation filtered with \u2018mkgtf\u2019 command of Cell Ranger options: \u2018\u2014attribute = gene biotype:lincRNA \u2013attribute = gene biotype:antisense  as well as the pseudo mTagBFP2  H2B mGL  mCherry NTR sequences in these cells using the mkref command. This index was used to map reads and generate gene expression matrices using the \u2018count\u2019 command of the Cell Ranger software v.7.1.0 provided by 10x Genomics with the option \u201c\u2013include  introns\u201d set to False all other options were used as per default. Assembly: GRCz11 Supplementary files format and content: Count Matrix as h5ad file Supplementary files format and content: Metadata listing the cell annotations as csv file", "Liver", null, "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer\u2019s instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell 3\u2019 v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer\u2019s protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Control \u2013 No 4 OHT", "GSM8403314", "GSM8403314: Liver Control1; Danio rerio; RNA Seq", "GSM8403314 r1", "GSM8403314", "1", "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer's instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell three prime v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer's protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 550", null, "SRP520597", null, "loader:fastq load.py", "ULB-CB-cr-zf-EES3-reload-EES1-larvlivCTL113dpf-GEM-230727_S19_L002_R2_001.fastq.gz ULB-CB-cr-zf-EES3-reload-EES1-larvlivCTL113dpf-GEM-230727_S19_L002_R1_001.fastq.gz ULB-CB-cr-zf-EES3-reload-EES1-larvlivCTL113dpf-GEM-230727_S19_L002_I2_001.fastq.gz ULB-CB-cr-zf-EES3-reload-EES1-larvlivCTL113dpf-GEM-230727_S19_L002_I1_001.fastq.gz", "fastq fastq fastq fastq", 11713305912.0, 69722059.0, "GSM8403314 r6", "0:10 1:10 2:28 3:120", "A:2418305019;C:1901514145;G:1976625286;T:2070035096;N:167534", 10, 10, 28, 120, 2418305019, 1901514145, 1976625286, 2070035096, 167534, "SRX25365551", "SRS22031292", "SRA1926595", "Regeneration and Stress Biology, IRIBHM, ULB", "Regeneration and Stress Biology, IRIBHM, ULB", 1, 0.92987, null, 0.09528, null, 0.8352, null, 0.60535, null, 120, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "Belgium", "2024-07-17", "Undetermined", "Larval", "Liver", "Liver and Biliary System"], [33252, "SRR29868329", "SRX25365551", "SRS22031292", "SRP520597", "PRJNA1136957", "Cholangiocytes contribute to hepatocyte regeneration post partial liver injury in larval zebrafish", "GSE272484", "Transcriptome Analysis", "Liver possesses robust regenerative ability  characterized by flexibility in the cellular source of regeneration based on the extent of the injury. post partial hepatectomy or minor injuries  hepatocytes  the primary liver cells  undergo self duplication to replenish the liver mass. In contrast  when the damage is extensive  or hepatocyte proliferation is impaired  cholangiocytes contribute to hepatocyte recovery. This current paradigm of regenerative flexibility in the liver has been established for animals with little or no growth. However  the regenerative mechanisms during periods of growth in young animals remain unexplored. Here  we establish two new partial liver injury protocols in the zebrafish model of rapid growth during late larval stage and observe emergence of de novo hepatocytes in the presence of spared hepatocytes. Using single cell RNA sequencing and lineage tracing  we identify cholangiocytes as the source of de novo hepatocytes. Our study offers a new perspective on the current paradigm of liver regenerating by proposing cholangiocyte to hepatocyte transdifferentiation as the default mechanism of hepatocyte recovery in late larval stage zebrafish. Overall design: We conducted single cell RNA Sequencing using the 10x platform of larval zebrafish liver. This was performed in the basal state Control1  Control2  post partial ablation MTZ 0dppa  MTZ 1dppa and MTZ 9dppa and post partial hepatectomy PHx 4dpi and PHx 11dpi. The two control samples were not treated with 4 OHT  and were also not injured. The control samples are from 13 dpf zebrafish raised under normal rearing condition. For all experiments Tgfabp10a:CellCousin line was used.", null, "pubmed:40480975", null, "Liver Control1", "GSM8403314", null, "source name:Liver|tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Control \u2013 No 4 OHT|geo loc name:missing|collection date:missing", "Liver Control1", "A custom Cell Ranger index was generated from the Ensembl GRCz11 genome sequence and annotation filtered with \u2018mkgtf\u2019 command of Cell Ranger options: \u2018\u2014attribute = gene biotype:lincRNA \u2013attribute = gene biotype:antisense  as well as the pseudo mTagBFP2  H2B mGL  mCherry NTR sequences in these cells using the mkref command. This index was used to map reads and generate gene expression matrices using the \u2018count\u2019 command of the Cell Ranger software v.7.1.0 provided by 10x Genomics with the option \u201c\u2013include  introns\u201d set to False all other options were used as per default. Assembly: GRCz11 Supplementary files format and content: Count Matrix as h5ad file Supplementary files format and content: Metadata listing the cell annotations as csv file", "Liver", null, "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer\u2019s instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell 3\u2019 v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer\u2019s protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Control \u2013 No 4 OHT", "GSM8403314", "GSM8403314: Liver Control1; Danio rerio; RNA Seq", "GSM8403314 r1", "GSM8403314", "1", "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer's instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell three prime v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer's protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 550", null, "SRP520597", null, "loader:fastq load.py", "ULB-CB-cr-zf-EES3-reload-EES1-larvlivCTL113dpf-GEM-230727_S19_L003_R2_001.fastq.gz ULB-CB-cr-zf-EES3-reload-EES1-larvlivCTL113dpf-GEM-230727_S19_L003_R1_001.fastq.gz ULB-CB-cr-zf-EES3-reload-EES1-larvlivCTL113dpf-GEM-230727_S19_L003_I2_001.fastq.gz ULB-CB-cr-zf-EES3-reload-EES1-larvlivCTL113dpf-GEM-230727_S19_L003_I1_001.fastq.gz", "fastq fastq fastq fastq", 12060725208.0, 71790031.0, "GSM8403314 r7", "0:10 1:10 2:28 3:120", "A:2488941083;C:1959911465;G:2036435394;T:2129304281;N:211497", 10, 10, 28, 120, 2488941083, 1959911465, 2036435394, 2129304281, 211497, "SRX25365551", "SRS22031292", "SRA1926595", "Regeneration and Stress Biology, IRIBHM, ULB", "Regeneration and Stress Biology, IRIBHM, ULB", 1, 0.92896, null, 0.09426, null, 0.83867, null, 0.53757, null, 120, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "Belgium", "2024-07-17", "Undetermined", "Larval", "Liver", "Liver and Biliary System"], [33253, "SRR29868330", "SRX25365551", "SRS22031292", "SRP520597", "PRJNA1136957", "Cholangiocytes contribute to hepatocyte regeneration post partial liver injury in larval zebrafish", "GSE272484", "Transcriptome Analysis", "Liver possesses robust regenerative ability  characterized by flexibility in the cellular source of regeneration based on the extent of the injury. post partial hepatectomy or minor injuries  hepatocytes  the primary liver cells  undergo self duplication to replenish the liver mass. In contrast  when the damage is extensive  or hepatocyte proliferation is impaired  cholangiocytes contribute to hepatocyte recovery. This current paradigm of regenerative flexibility in the liver has been established for animals with little or no growth. However  the regenerative mechanisms during periods of growth in young animals remain unexplored. Here  we establish two new partial liver injury protocols in the zebrafish model of rapid growth during late larval stage and observe emergence of de novo hepatocytes in the presence of spared hepatocytes. Using single cell RNA sequencing and lineage tracing  we identify cholangiocytes as the source of de novo hepatocytes. Our study offers a new perspective on the current paradigm of liver regenerating by proposing cholangiocyte to hepatocyte transdifferentiation as the default mechanism of hepatocyte recovery in late larval stage zebrafish. Overall design: We conducted single cell RNA Sequencing using the 10x platform of larval zebrafish liver. This was performed in the basal state Control1  Control2  post partial ablation MTZ 0dppa  MTZ 1dppa and MTZ 9dppa and post partial hepatectomy PHx 4dpi and PHx 11dpi. The two control samples were not treated with 4 OHT  and were also not injured. The control samples are from 13 dpf zebrafish raised under normal rearing condition. For all experiments Tgfabp10a:CellCousin line was used.", null, "pubmed:40480975", null, "Liver Control1", "GSM8403314", null, "source name:Liver|tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Control \u2013 No 4 OHT|geo loc name:missing|collection date:missing", "Liver Control1", "A custom Cell Ranger index was generated from the Ensembl GRCz11 genome sequence and annotation filtered with \u2018mkgtf\u2019 command of Cell Ranger options: \u2018\u2014attribute = gene biotype:lincRNA \u2013attribute = gene biotype:antisense  as well as the pseudo mTagBFP2  H2B mGL  mCherry NTR sequences in these cells using the mkref command. This index was used to map reads and generate gene expression matrices using the \u2018count\u2019 command of the Cell Ranger software v.7.1.0 provided by 10x Genomics with the option \u201c\u2013include  introns\u201d set to False all other options were used as per default. Assembly: GRCz11 Supplementary files format and content: Count Matrix as h5ad file Supplementary files format and content: Metadata listing the cell annotations as csv file", "Liver", null, "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer\u2019s instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell 3\u2019 v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer\u2019s protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "tissue:Liver|transgene:Tgfabp10a:CellCousin|treatment:Control \u2013 No 4 OHT", "GSM8403314", "GSM8403314: Liver Control1; Danio rerio; RNA Seq", "GSM8403314 r1", "GSM8403314", "1", "Zebrafish were euthanized in 500 mg/l solution of tricaine MS 222 Sigma Aldrich  E10521. The entire liver was dissected out of the body using Dumont #5 forceps Fine Science Tools  11295 10. All steps for making the single cell suspension were performed with tubes and pipette tips coated with 1% BSA in PBS prior to the sample preparation. Briefly  the liver was dissociated into single cells by incubation it in TrypLE Thermo Fisher  12563029 at 37\u00b0C in a benchtop shaker set at 1000 rpm for 15 min. Following dissociation  TrypLE was inactivated with goat serum. To remove undissociated chunks and debris  the solution was passed through a 40 \u03bcm cell strainer Miltenyi Biotec  130 041 407. Cells were pelleted by centrifugation at 500 g for 5 min at 4\u00b0C with soft stop setting. The supernatant was carefully discarded and the pellet re suspended in 500 \u03bcl of PBS. To remove dead cells  Calcein violet Thermo Fisher  C34858 or Draq7 Thermo Fisher  D15105 was added at a final concentration of 1 \u00b5M and 3 \u00b5M  respectively  and the cell suspension was incubated at room temperature for 20 min. The single cell preparation was sorted using appropriate gates  including excitation with UV 405 nm or a 633 nm laser for identifying live cells with Calcein+ or Draq7   respectively. FACS was performed  using a 100 \u00b5m nozzle  and a minimum of 15 000 live cells per condition were collected into BSA coated tubes containing 1% BSA in PBS. Sorting time did not exceed 15 min. For single cell RNA sequencing using the 10x Genomics platform  the cell suspension was adjusted with PBS to a density of 300 cells/\u00b5l and diluted with nuclease free water according to the manufacturer's instructions to yield 7 000 cells. Subsequently  the cells were carefully mixed with reverse transcription mix before loading the cells on the 10x Genomics Chromium system Single Cell three prime v3. post the gel emulsion bead suspension underwent the reverse transcription reaction  emulsion was broken and DNA purified using Silane beads. The complementary DNA was amplified with 12 cycles  following the guidelines of the 10x Genomics user manual. The 10x Genomics single cell RNA seq library preparation\u2014involving fragmentation  dA tailing  adapter ligation  and indexing PCR\u2014was performed based on the manufacturer's protocol. post quantification  the libraries were sequenced on an Illumina NextSeq 550 machine.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 550", null, "SRP520597", null, "loader:fastq load.py", "ULB-CB-cr-zf-EES3-reload-EES1-larvlivCTL113dpf-GEM-230727_S19_L004_I1_001.fastq.gz ULB-CB-cr-zf-EES3-reload-EES1-larvlivCTL113dpf-GEM-230727_S19_L004_I2_001.fastq.gz ULB-CB-cr-zf-EES3-reload-EES1-larvlivCTL113dpf-GEM-230727_S19_L004_R1_001.fastq.gz ULB-CB-cr-zf-EES3-reload-EES1-larvlivCTL113dpf-GEM-230727_S19_L004_R2_001.fastq.gz", "fastq fastq fastq fastq", 11892922944.0, 70791208.0, "GSM8403314 r8", "0:10 1:10 2:28 3:120", "A:2456445818;C:1931328609;G:2006328383;T:2100554789;N:287361", 10, 10, 28, 120, 2456445818, 1931328609, 2006328383, 2100554789, 287361, "SRX25365551", "SRS22031292", "SRA1926595", "Regeneration and Stress Biology, IRIBHM, ULB", "Regeneration and Stress Biology, IRIBHM, ULB", 1, 0.92946, null, 0.09343, null, 0.83704, null, 0.59688, null, 120, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "Belgium", "2024-07-17", "Undetermined", "Larval", "Liver", "Liver and Biliary System"], [70706, "SRR21457012", "SRX17460721", "SRS15014532", "SRP385641", "PRJNA857143", "Single cell transcriptomic data of zebrafish lbw mutant", "PRJNA857143", "Other", "Zebrafish lbw mutant exhibits hepatized anterior intestine. To characterize the functional liver cells in lbw intestine  we dissected lbw intestine at 6 dpf and carried out scRNA seq to compare its cell population clusters with wild type liver and intestine.", null, null, null, null, "Liver in the WT S4 L004", null, "isolate:wild type|dev stage:6 dpf|sex:female and male|tissue:liver|aliquot:aliquot 4|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "scRNA Seq of zebrafish: 6 dpf wild type liver", "Live S4 L004  aliquot 4", "Live S4 L004  aliquot 4", "using Chromium Single Cell three prime GEM v3.1 Reagent Kit", null, null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "Oligo-dT", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP385641", null, null, "20200806_S4_Live_20200825NB_S4_L004_I1_001.fastq.gz 20200806_S4_Live_20200825NB_S4_L004_R1_001.fastq.gz 20200806_S4_Live_20200825NB_S4_L004_R2_001.fastq.gz", "fastq fastq fastq", 15359722785.0, 82137555.0, "20200806 S4 Live 20200825NB S4 L004 I1 001.fastq.gz", "0:8 1:28 2:151", "A:3860252969;C:2756020453;G:2781498176;T:3004915512;N:83695", 8, 28, 151, null, 3860252969, 2756020453, 2781498176, 3004915512, 83695, "SRX17460721", "SRS15014532", "SRA1491985", "Southwest University|Institute of Developmental Biology and Regenerativ", "Southwest University", 1, 0.90519, null, 0.06757, null, 0.88243, null, 0.74961, null, 151, null, "B", null, "usable mapping rate", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_droplet", "10x", null, "China", "2022-09-11", "Larval", "Larval", "Liver", "Liver and Biliary System"], [70707, "SRR21457013", "SRX17460720", "SRS15014531", "SRP385641", "PRJNA857143", "Single cell transcriptomic data of zebrafish lbw mutant", "PRJNA857143", "Other", "Zebrafish lbw mutant exhibits hepatized anterior intestine. To characterize the functional liver cells in lbw intestine  we dissected lbw intestine at 6 dpf and carried out scRNA seq to compare its cell population clusters with wild type liver and intestine.", null, null, null, null, "Liver in the WT S4 L003", null, "isolate:wild type|dev stage:6 dpf|sex:female and male|tissue:liver|aliquot:aliquot 3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "scRNA Seq of zebrafish: 6 dpf wild type liver", "Live S4 L003  aliquot 3", "Live S4 L003  aliquot 3", "using Chromium Single Cell three prime GEM v3.1 Reagent Kit", null, null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "Oligo-dT", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP385641", null, null, "20200806_S4_Live_20200825NB_S4_L003_I1_001.fastq.gz 20200806_S4_Live_20200825NB_S4_L003_R1_001.fastq.gz 20200806_S4_Live_20200825NB_S4_L003_R2_001.fastq.gz", "fastq fastq fastq", 15277905984.0, 81700032.0, "20200806 S4 Live 20200825NB S4 L003 I1 001.fastq.gz", "0:8 1:28 2:151", "A:3834498109;C:2745604041;G:2766967723;T:2989497144;N:137815", 8, 28, 151, null, 3834498109, 2745604041, 2766967723, 2989497144, 137815, "SRX17460720", "SRS15014531", "SRA1491985", "Southwest University|Institute of Developmental Biology and Regenerativ", "Southwest University", 1, 0.90792, null, 0.06795, null, 0.88089, null, 0.75291, null, 151, null, "B", null, "usable mapping rate", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_droplet", "10x", null, "China", "2022-09-11", "Larval", "Larval", "Liver", "Liver and Biliary System"], [70710, "SRR21457016", "SRX17460717", "SRS15014528", "SRP385641", "PRJNA857143", "Single cell transcriptomic data of zebrafish lbw mutant", "PRJNA857143", "Other", "Zebrafish lbw mutant exhibits hepatized anterior intestine. To characterize the functional liver cells in lbw intestine  we dissected lbw intestine at 6 dpf and carried out scRNA seq to compare its cell population clusters with wild type liver and intestine.", null, null, null, null, "Liver in the WT S4 L002", null, "isolate:wild type|dev stage:6 dpf|sex:female and male|tissue:liver|aliquot:aliquot 2|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "scRNA Seq of zebrafish: 6 dpf wild type liver", "Live S4 L002  aliquot 2", "Live S4 L002  aliquot 2", "using Chromium Single Cell three prime GEM v3.1 Reagent Kit", null, null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "Oligo-dT", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP385641", null, null, "20200806_S4_Live_20200825NB_S4_L002_I1_001.fastq.gz 20200806_S4_Live_20200825NB_S4_L002_R1_001.fastq.gz 20200806_S4_Live_20200825NB_S4_L002_R2_001.fastq.gz", "fastq fastq fastq", 15033903335.0, 80395205.0, "20200806 S4 Live 20200825NB S4 L002 I1 001.fastq.gz", "0:8 1:28 2:151", "A:3774023536;C:2701104584;G:2719224706;T:2945204367;N:118762", 8, 28, 151, null, 3774023536, 2701104584, 2719224706, 2945204367, 118762, "SRX17460717", "SRS15014528", "SRA1491985", "Southwest University|Institute of Developmental Biology and Regenerativ", "Southwest University", 1, 0.90993, null, 0.06896, null, 0.88002, null, 0.75003, null, 151, null, "B", null, "usable mapping rate", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_droplet", "10x", null, "China", "2022-09-11", "Larval", "Larval", "Liver", "Liver and Biliary System"], [70711, "SRR21457017", "SRX17460716", "SRS15014527", "SRP385641", "PRJNA857143", "Single cell transcriptomic data of zebrafish lbw mutant", "PRJNA857143", "Other", "Zebrafish lbw mutant exhibits hepatized anterior intestine. To characterize the functional liver cells in lbw intestine  we dissected lbw intestine at 6 dpf and carried out scRNA seq to compare its cell population clusters with wild type liver and intestine.", null, null, null, null, "Liver in the WT S4 L001", null, "isolate:wild type|dev stage:6 dpf|sex:female and male|tissue:liver|aliquot:aliquot 1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "scRNA Seq of zebrafish: 6 dpf wild type liver", "Live S4 L001  aliquot 1", "Live S4 L001  aliquot 1", "using Chromium Single Cell three prime GEM v3.1 Reagent Kit", null, null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "Oligo-dT", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP385641", null, null, "20200806_S4_Live_20200825NB_S4_L001_I1_001.fastq.gz 20200806_S4_Live_20200825NB_S4_L001_R1_001.fastq.gz 20200806_S4_Live_20200825NB_S4_L001_R2_001.fastq.gz", "fastq fastq fastq", 15047379303.0, 80467269.0, "20200806 S4 Live 20200825NB S4 L001 I1 001.fastq.gz", "0:8 1:28 2:151", "A:3778269770;C:2702450055;G:2724848747;T:2944890394;N:98653", 8, 28, 151, null, 3778269770, 2702450055, 2724848747, 2944890394, 98653, "SRX17460716", "SRS15014527", "SRA1491985", "Southwest University|Institute of Developmental Biology and Regenerativ", "Southwest University", 1, 0.90977, null, 0.06799, null, 0.87998, null, 0.75241, null, 151, null, "B", null, "usable mapping rate", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_droplet", "10x", null, "China", "2022-09-11", "Larval", "Larval", "Liver", "Liver and Biliary System"], [72137, "SRR22266708", "SRX18243019", "SRS15738744", "SRP407581", "PRJNA900999", "Biliary epithelial cells are facultative liver stem cells during liver regeneration in adult zebrafish", "GSE217839", "Transcriptome Analysis", "Single cell sequencing was performed on the adult zebrafish liver  on both uninjured animals  and animals regenerating from hepatocyte ablation. Overall design: Samples were sequenced at 0  1  2  3  and 7 days post ablation. Mock treated and uninjured animals were also sequenced.", null, "pubmed:36625346", null, "7 dpa  replicate 31  scRNAseq", "GSM6727464", null, "source name:liver|tissue:liver|cell type:mCherry+ cells|genotype:TgTp1:CreERT2  Tg 3.5ubb:LOXP EGFP LOXP mCherry  Tgfabp10a:CFP NTR|treatment:7 dpa", "7 dpa  replicate 31  scRNAseq", "Fastq files were processed as described using the 2.4.0 version of the Drop seq tools  mapping the reads to GRCz11 with the default parameters. For the DigitalExpression command we set NUM CORE BARCODES=5000. We used STAR version 2.5.3 and fastqc version 0.11.2. Assembly: GRCz11 Supplementary files format and content: *.txt.gz Supplementary files format and content: Digital Gene Expression Matrix", "liver", null, "Adult zebrafish livers were enyzmatically dissociated and live  nucleated cells were enriched using FACS. The resultant single cell suspension was subjected to the Drop seq protocol. Beads were subjected to reverse transcription with Maxima H Minus Reverse Transcripase. cDNA was amplified with KAPA HiFi HotStart ReadyMix. Amplified cDNA was made into libraries using the Nextera XT DNA Library Preparation Kit  with the goal of capturing 1000 cells per library. Libraries were sequenced on an Illumina NextSeq 500 using the Single End 75bp kit with the following parameters: Read 1: 20 bp  Read 2: 50 bp  Read 1 Index: 8 bp  Custom Read 1 primer  GCCTGTCCGCGGAAGCAGTGGTATCAACGCAGAGTAC.", null, "tissue:liver|cell type:mCherry+ cells|genotype:TgTp1:CreERT2  Tg 3.5ubb:LOXP EGFP LOXP mCherry  Tgfabp10a:CFP NTR|treatment:7 dpa", "GSM6727464", "GSM6727464: 7 dpa  replicate 31  scRNAseq; Danio rerio; RNA Seq", "GSM6727464 r1", "GSM6727464", "1", "Adult zebrafish livers were enyzmatically dissociated and live  nucleated cells were enriched using FACS. The resultant single cell suspension was subjected to the Drop seq protocol. Beads were subjected to reverse transcription with Maxima H Minus Reverse Transcripase. cDNA was amplified with KAPA HiFi HotStart ReadyMix. Amplified cDNA was made into libraries using the Nextera XT DNA Library Preparation Kit  with the goal of capturing 1000 cells per library. Libraries were sequenced on an Illumina NextSeq 500 using the Single End 75bp kit with the following parameters: Read 1: 20 bp  Read 2: 50 bp  Read 1 Index: 8 bp  Custom Read 1 primer  GCCTGTCCGCGGAAGCAGTGGTATCAACGCAGAGTAC.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 500", null, "SRP407581", null, null, "20200107_LIB11R_IO7720_S6_R1_001.fastq.gz 20200107_LIB11R_IO7720_S6_R2_001.fastq.gz", "fastq fastq", 4802290236.0, 64895814.0, "GSM6727464 r1", "0:25 1:49", "A:1351689314;C:1044099826;G:1084104790;T:1321739644;N:656662", 25, 49, null, null, 1351689314, 1044099826, 1084104790, 1321739644, 656662, "SRX18243019", "SRS15738744", null, null, "Division of Genetics, Brigham and Women's Hospital", 2, 0.01055, 0.84852, 0.00192, 0.03717, 0.99527, 0.89234, 0.86731, 0.69661, 25, 49, "T", "B", "sc-like readlen", "illumina", "nextseq", "unknown", "cdna_unspecified", "nextera", "sc", "single_cell_droplet", "dropseq", null, "United States", "2022-11-12", "Adult", "Adult", "Liver", "Liver and Biliary System"], [72138, "SRR22266709", "SRX18243018", "SRS15738743", "SRP407581", "PRJNA900999", "Biliary epithelial cells are facultative liver stem cells during liver regeneration in adult zebrafish", "GSE217839", "Transcriptome Analysis", "Single cell sequencing was performed on the adult zebrafish liver  on both uninjured animals  and animals regenerating from hepatocyte ablation. Overall design: Samples were sequenced at 0  1  2  3  and 7 days post ablation. Mock treated and uninjured animals were also sequenced.", null, "pubmed:36625346", null, "3 dpa  replicate 3  scRNAseq", "GSM6727463", null, "source name:liver|tissue:liver|cell type:mCherry+ cells|genotype:TgTp1:CreERT2  Tg 3.5ubb:LOXP EGFP LOXP mCherry  Tgfabp10a:CFP NTR|treatment:3 dpa", "3 dpa  replicate 3  scRNAseq", "Fastq files were processed as described using the 2.4.0 version of the Drop seq tools  mapping the reads to GRCz11 with the default parameters. For the DigitalExpression command we set NUM CORE BARCODES=5000. We used STAR version 2.5.3 and fastqc version 0.11.2. Assembly: GRCz11 Supplementary files format and content: *.txt.gz Supplementary files format and content: Digital Gene Expression Matrix", "liver", null, "Adult zebrafish livers were enyzmatically dissociated and live  nucleated cells were enriched using FACS. The resultant single cell suspension was subjected to the Drop seq protocol. Beads were subjected to reverse transcription with Maxima H Minus Reverse Transcripase. cDNA was amplified with KAPA HiFi HotStart ReadyMix. Amplified cDNA was made into libraries using the Nextera XT DNA Library Preparation Kit  with the goal of capturing 1000 cells per library. Libraries were sequenced on an Illumina NextSeq 500 using the Single End 75bp kit with the following parameters: Read 1: 20 bp  Read 2: 50 bp  Read 1 Index: 8 bp  Custom Read 1 primer  GCCTGTCCGCGGAAGCAGTGGTATCAACGCAGAGTAC.", null, "tissue:liver|cell type:mCherry+ cells|genotype:TgTp1:CreERT2  Tg 3.5ubb:LOXP EGFP LOXP mCherry  Tgfabp10a:CFP NTR|treatment:3 dpa", "GSM6727463", "GSM6727463: 3 dpa  replicate 3  scRNAseq; Danio rerio; RNA Seq", "GSM6727463 r1", "GSM6727463", "1", "Adult zebrafish livers were enyzmatically dissociated and live  nucleated cells were enriched using FACS. The resultant single cell suspension was subjected to the Drop seq protocol. Beads were subjected to reverse transcription with Maxima H Minus Reverse Transcripase. cDNA was amplified with KAPA HiFi HotStart ReadyMix. Amplified cDNA was made into libraries using the Nextera XT DNA Library Preparation Kit  with the goal of capturing 1000 cells per library. Libraries were sequenced on an Illumina NextSeq 500 using the Single End 75bp kit with the following parameters: Read 1: 20 bp  Read 2: 50 bp  Read 1 Index: 8 bp  Custom Read 1 primer  GCCTGTCCGCGGAAGCAGTGGTATCAACGCAGAGTAC.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 500", null, "SRP407581", null, null, "20200107_LIB11Q_IO7720_S5_R1_001.fastq.gz 20200107_LIB11Q_IO7720_S5_R2_001.fastq.gz", "fastq fastq", 4327529324.0, 58480126.0, "GSM6727463 r1", "0:25 1:49", "A:1223359778;C:914892543;G:970000561;T:1218683252;N:593190", 25, 49, null, null, 1223359778, 914892543, 970000561, 1218683252, 593190, "SRX18243018", "SRS15738743", null, null, "Division of Genetics, Brigham and Women's Hospital", 2, 0.00899, 0.83745, 0.00188, 0.04633, 0.99431, 0.84492, 0.84148, 0.68261, 25, 49, "T", "B", "sc-like readlen", "illumina", "nextseq", "unknown", "cdna_unspecified", "nextera", "sc", "single_cell_droplet", "dropseq", null, "United States", "2022-11-12", "Adult", "Adult", "Liver", "Liver and Biliary System"], [72139, "SRR22266710", "SRX18243017", "SRS15738742", "SRP407581", "PRJNA900999", "Biliary epithelial cells are facultative liver stem cells during liver regeneration in adult zebrafish", "GSE217839", "Transcriptome Analysis", "Single cell sequencing was performed on the adult zebrafish liver  on both uninjured animals  and animals regenerating from hepatocyte ablation. Overall design: Samples were sequenced at 0  1  2  3  and 7 days post ablation. Mock treated and uninjured animals were also sequenced.", null, "pubmed:36625346", null, "2 dpa  replicate 3  scRNAseq", "GSM6727462", null, "source name:liver|tissue:liver|cell type:mCherry+ cells|genotype:TgTp1:CreERT2  Tg 3.5ubb:LOXP EGFP LOXP mCherry  Tgfabp10a:CFP NTR|treatment:2 dpa", "2 dpa  replicate 3  scRNAseq", "Fastq files were processed as described using the 2.4.0 version of the Drop seq tools  mapping the reads to GRCz11 with the default parameters. For the DigitalExpression command we set NUM CORE BARCODES=5000. We used STAR version 2.5.3 and fastqc version 0.11.2. Assembly: GRCz11 Supplementary files format and content: *.txt.gz Supplementary files format and content: Digital Gene Expression Matrix", "liver", null, "Adult zebrafish livers were enyzmatically dissociated and live  nucleated cells were enriched using FACS. The resultant single cell suspension was subjected to the Drop seq protocol. Beads were subjected to reverse transcription with Maxima H Minus Reverse Transcripase. cDNA was amplified with KAPA HiFi HotStart ReadyMix. Amplified cDNA was made into libraries using the Nextera XT DNA Library Preparation Kit  with the goal of capturing 1000 cells per library. Libraries were sequenced on an Illumina NextSeq 500 using the Single End 75bp kit with the following parameters: Read 1: 20 bp  Read 2: 50 bp  Read 1 Index: 8 bp  Custom Read 1 primer  GCCTGTCCGCGGAAGCAGTGGTATCAACGCAGAGTAC.", null, "tissue:liver|cell type:mCherry+ cells|genotype:TgTp1:CreERT2  Tg 3.5ubb:LOXP EGFP LOXP mCherry  Tgfabp10a:CFP NTR|treatment:2 dpa", "GSM6727462", "GSM6727462: 2 dpa  replicate 3  scRNAseq; Danio rerio; RNA Seq", "GSM6727462 r1", "GSM6727462", "1", "Adult zebrafish livers were enyzmatically dissociated and live  nucleated cells were enriched using FACS. The resultant single cell suspension was subjected to the Drop seq protocol. Beads were subjected to reverse transcription with Maxima H Minus Reverse Transcripase. cDNA was amplified with KAPA HiFi HotStart ReadyMix. Amplified cDNA was made into libraries using the Nextera XT DNA Library Preparation Kit  with the goal of capturing 1000 cells per library. Libraries were sequenced on an Illumina NextSeq 500 using the Single End 75bp kit with the following parameters: Read 1: 20 bp  Read 2: 50 bp  Read 1 Index: 8 bp  Custom Read 1 primer  GCCTGTCCGCGGAAGCAGTGGTATCAACGCAGAGTAC.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 500", null, "SRP407581", null, null, "20200107_LIB11P_IO7720_S4_R1_001.fastq.gz 20200107_LIB11P_IO7720_S4_R2_001.fastq.gz", "fastq fastq", 5036062970.0, 68054905.0, "GSM6727462 r1", "0:25 1:49", "A:1410235407;C:1062242143;G:1134885588;T:1428020401;N:679431", 25, 49, null, null, 1410235407, 1062242143, 1134885588, 1428020401, 679431, "SRX18243017", "SRS15738742", null, null, "Division of Genetics, Brigham and Women's Hospital", 2, 0.00859, 0.86477, 0.00193, 0.06588, 0.99387, 0.81124, 0.81235, 0.61962, 25, 49, "T", "B", "sc-like readlen", "illumina", "nextseq", "unknown", "cdna_unspecified", "nextera", "sc", "single_cell_droplet", "dropseq", null, "United States", "2022-11-12", "Adult", "Adult", "Liver", "Liver and Biliary System"], [72140, "SRR22266711", "SRX18243016", "SRS15738741", "SRP407581", "PRJNA900999", "Biliary epithelial cells are facultative liver stem cells during liver regeneration in adult zebrafish", "GSE217839", "Transcriptome Analysis", "Single cell sequencing was performed on the adult zebrafish liver  on both uninjured animals  and animals regenerating from hepatocyte ablation. Overall design: Samples were sequenced at 0  1  2  3  and 7 days post ablation. Mock treated and uninjured animals were also sequenced.", null, "pubmed:36625346", null, "1 dpa  replicate 3  scRNAseq", "GSM6727461", null, "source name:liver|tissue:liver|cell type:mCherry+ cells|genotype:TgTp1:CreERT2  Tg 3.5ubb:LOXP EGFP LOXP mCherry  Tgfabp10a:CFP NTR|treatment:1 dpa", "1 dpa  replicate 3  scRNAseq", "Fastq files were processed as described using the 2.4.0 version of the Drop seq tools  mapping the reads to GRCz11 with the default parameters. For the DigitalExpression command we set NUM CORE BARCODES=5000. We used STAR version 2.5.3 and fastqc version 0.11.2. Assembly: GRCz11 Supplementary files format and content: *.txt.gz Supplementary files format and content: Digital Gene Expression Matrix", "liver", null, "Adult zebrafish livers were enyzmatically dissociated and live  nucleated cells were enriched using FACS. The resultant single cell suspension was subjected to the Drop seq protocol. Beads were subjected to reverse transcription with Maxima H Minus Reverse Transcripase. cDNA was amplified with KAPA HiFi HotStart ReadyMix. Amplified cDNA was made into libraries using the Nextera XT DNA Library Preparation Kit  with the goal of capturing 1000 cells per library. Libraries were sequenced on an Illumina NextSeq 500 using the Single End 75bp kit with the following parameters: Read 1: 20 bp  Read 2: 50 bp  Read 1 Index: 8 bp  Custom Read 1 primer  GCCTGTCCGCGGAAGCAGTGGTATCAACGCAGAGTAC.", null, "tissue:liver|cell type:mCherry+ cells|genotype:TgTp1:CreERT2  Tg 3.5ubb:LOXP EGFP LOXP mCherry  Tgfabp10a:CFP NTR|treatment:1 dpa", "GSM6727461", "GSM6727461: 1 dpa  replicate 3  scRNAseq; Danio rerio; RNA Seq", "GSM6727461 r1", "GSM6727461", "1", "Adult zebrafish livers were enyzmatically dissociated and live  nucleated cells were enriched using FACS. The resultant single cell suspension was subjected to the Drop seq protocol. Beads were subjected to reverse transcription with Maxima H Minus Reverse Transcripase. cDNA was amplified with KAPA HiFi HotStart ReadyMix. Amplified cDNA was made into libraries using the Nextera XT DNA Library Preparation Kit  with the goal of capturing 1000 cells per library. Libraries were sequenced on an Illumina NextSeq 500 using the Single End 75bp kit with the following parameters: Read 1: 20 bp  Read 2: 50 bp  Read 1 Index: 8 bp  Custom Read 1 primer  GCCTGTCCGCGGAAGCAGTGGTATCAACGCAGAGTAC.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 500", null, "SRP407581", null, null, "20200107_LIB11O_IO7720_S3_R1_001.fastq.gz 20200107_LIB11O_IO7720_S3_R2_001.fastq.gz", "fastq fastq", 4734982870.0, 63986255.0, "GSM6727461 r1", "0:25 1:49", "A:1350059086;C:986396540;G:1055462585;T:1342412482;N:652177", 25, 49, null, null, 1350059086, 986396540, 1055462585, 1342412482, 652177, "SRX18243016", "SRS15738741", null, null, "Division of Genetics, Brigham and Women's Hospital", 2, 0.01072, 0.82414, 0.0025, 0.06977, 0.99159, 0.83004, 0.66346, 0.59359, 25, 49, "T", "B", "sc-like readlen", "illumina", "nextseq", "unknown", "cdna_unspecified", "nextera", "sc", "single_cell_droplet", "dropseq", null, "United States", "2022-11-12", "Adult", "Adult", "Liver", "Liver and Biliary System"], [72141, "SRR22266712", "SRX18243015", "SRS15738740", "SRP407581", "PRJNA900999", "Biliary epithelial cells are facultative liver stem cells during liver regeneration in adult zebrafish", "GSE217839", "Transcriptome Analysis", "Single cell sequencing was performed on the adult zebrafish liver  on both uninjured animals  and animals regenerating from hepatocyte ablation. Overall design: Samples were sequenced at 0  1  2  3  and 7 days post ablation. Mock treated and uninjured animals were also sequenced.", null, "pubmed:36625346", null, "0 dpa  replicate 3  scRNAseq", "GSM6727460", null, "source name:liver|tissue:liver|cell type:mCherry+ cells|genotype:TgTp1:CreERT2  Tg 3.5ubb:LOXP EGFP LOXP mCherry  Tgfabp10a:CFP NTR|treatment:0 dpa", "0 dpa  replicate 3  scRNAseq", "Fastq files were processed as described using the 2.4.0 version of the Drop seq tools  mapping the reads to GRCz11 with the default parameters. For the DigitalExpression command we set NUM CORE BARCODES=5000. We used STAR version 2.5.3 and fastqc version 0.11.2. Assembly: GRCz11 Supplementary files format and content: *.txt.gz Supplementary files format and content: Digital Gene Expression Matrix", "liver", null, "Adult zebrafish livers were enyzmatically dissociated and live  nucleated cells were enriched using FACS. The resultant single cell suspension was subjected to the Drop seq protocol. Beads were subjected to reverse transcription with Maxima H Minus Reverse Transcripase. cDNA was amplified with KAPA HiFi HotStart ReadyMix. Amplified cDNA was made into libraries using the Nextera XT DNA Library Preparation Kit  with the goal of capturing 1000 cells per library. Libraries were sequenced on an Illumina NextSeq 500 using the Single End 75bp kit with the following parameters: Read 1: 20 bp  Read 2: 50 bp  Read 1 Index: 8 bp  Custom Read 1 primer  GCCTGTCCGCGGAAGCAGTGGTATCAACGCAGAGTAC.", null, "tissue:liver|cell type:mCherry+ cells|genotype:TgTp1:CreERT2  Tg 3.5ubb:LOXP EGFP LOXP mCherry  Tgfabp10a:CFP NTR|treatment:0 dpa", "GSM6727460", "GSM6727460: 0 dpa  replicate 3  scRNAseq; Danio rerio; RNA Seq", "GSM6727460 r1", "GSM6727460", "1", "Adult zebrafish livers were enyzmatically dissociated and live  nucleated cells were enriched using FACS. The resultant single cell suspension was subjected to the Drop seq protocol. Beads were subjected to reverse transcription with Maxima H Minus Reverse Transcripase. cDNA was amplified with KAPA HiFi HotStart ReadyMix. Amplified cDNA was made into libraries using the Nextera XT DNA Library Preparation Kit  with the goal of capturing 1000 cells per library. Libraries were sequenced on an Illumina NextSeq 500 using the Single End 75bp kit with the following parameters: Read 1: 20 bp  Read 2: 50 bp  Read 1 Index: 8 bp  Custom Read 1 primer  GCCTGTCCGCGGAAGCAGTGGTATCAACGCAGAGTAC.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 500", null, "SRP407581", null, null, "20200107_LIB11N_IO7720_S2_R1_001.fastq.gz 20200107_LIB11N_IO7720_S2_R2_001.fastq.gz", "fastq fastq", 4224737108.0, 57091042.0, "GSM6727460 r1", "0:25 1:49", "A:1171021854;C:919004861;G:955423740;T:1178700581;N:586072", 25, 49, null, null, 1171021854, 919004861, 955423740, 1178700581, 586072, "SRX18243015", "SRS15738740", null, null, "Division of Genetics, Brigham and Women's Hospital", 2, 0.00691, 0.71165, 0.00186, 0.09225, 0.99634, 0.86354, 0.77015, 0.67507, 25, 49, "T", "B", "sc-like readlen", "illumina", "nextseq", "unknown", "cdna_unspecified", "nextera", "sc", "single_cell_droplet", "dropseq", null, "United States", "2022-11-12", "Adult", "Adult", "Liver", "Liver and Biliary System"], [72142, "SRR22266713", "SRX18243014", "SRS15738739", "SRP407581", "PRJNA900999", "Biliary epithelial cells are facultative liver stem cells during liver regeneration in adult zebrafish", "GSE217839", "Transcriptome Analysis", "Single cell sequencing was performed on the adult zebrafish liver  on both uninjured animals  and animals regenerating from hepatocyte ablation. Overall design: Samples were sequenced at 0  1  2  3  and 7 days post ablation. Mock treated and uninjured animals were also sequenced.", null, "pubmed:36625346", null, "mock  replicate 3  scRNAseq", "GSM6727459", null, "source name:liver|tissue:liver|cell type:mCherry+ cells|genotype:TgTp1:CreERT2  Tg 3.5ubb:LOXP EGFP LOXP mCherry  Tgfabp10a:CFP NTR|treatment:mock", "mock  replicate 3  scRNAseq", "Fastq files were processed as described using the 2.4.0 version of the Drop seq tools  mapping the reads to GRCz11 with the default parameters. For the DigitalExpression command we set NUM CORE BARCODES=5000. We used STAR version 2.5.3 and fastqc version 0.11.2. Assembly: GRCz11 Supplementary files format and content: *.txt.gz Supplementary files format and content: Digital Gene Expression Matrix", "liver", null, "Adult zebrafish livers were enyzmatically dissociated and live  nucleated cells were enriched using FACS. The resultant single cell suspension was subjected to the Drop seq protocol. Beads were subjected to reverse transcription with Maxima H Minus Reverse Transcripase. cDNA was amplified with KAPA HiFi HotStart ReadyMix. Amplified cDNA was made into libraries using the Nextera XT DNA Library Preparation Kit  with the goal of capturing 1000 cells per library. Libraries were sequenced on an Illumina NextSeq 500 using the Single End 75bp kit with the following parameters: Read 1: 20 bp  Read 2: 50 bp  Read 1 Index: 8 bp  Custom Read 1 primer  GCCTGTCCGCGGAAGCAGTGGTATCAACGCAGAGTAC.", null, "tissue:liver|cell type:mCherry+ cells|genotype:TgTp1:CreERT2  Tg 3.5ubb:LOXP EGFP LOXP mCherry  Tgfabp10a:CFP NTR|treatment:mock", "GSM6727459", "GSM6727459: mock  replicate 3  scRNAseq; Danio rerio; RNA Seq", "GSM6727459 r1", "GSM6727459", "1", "Adult zebrafish livers were enyzmatically dissociated and live  nucleated cells were enriched using FACS. The resultant single cell suspension was subjected to the Drop seq protocol. Beads were subjected to reverse transcription with Maxima H Minus Reverse Transcripase. cDNA was amplified with KAPA HiFi HotStart ReadyMix. Amplified cDNA was made into libraries using the Nextera XT DNA Library Preparation Kit  with the goal of capturing 1000 cells per library. Libraries were sequenced on an Illumina NextSeq 500 using the Single End 75bp kit with the following parameters: Read 1: 20 bp  Read 2: 50 bp  Read 1 Index: 8 bp  Custom Read 1 primer  GCCTGTCCGCGGAAGCAGTGGTATCAACGCAGAGTAC.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 500", null, "SRP407581", null, null, "20200107_LIB11M_IO7720_S1_R1_001.fastq.gz 20200107_LIB11M_IO7720_S1_R2_001.fastq.gz", "fastq fastq", 4136407822.0, 55897403.0, "GSM6727459 r1", "0:25 1:49", "A:1196087924;C:868741250;G:928417059;T:1142590047;N:571542", 25, 49, null, null, 1196087924, 868741250, 928417059, 1142590047, 571542, "SRX18243014", "SRS15738739", null, null, "Division of Genetics, Brigham and Women's Hospital", 2, 0.00992, 0.78, 0.00257, 0.11335, 0.99431, 0.83747, 0.75089, 0.69588, 25, 49, "T", "B", "sc-like readlen", "illumina", "nextseq", "unknown", "cdna_unspecified", "nextera", "sc", "single_cell_droplet", "dropseq", null, "United States", "2022-11-12", "Adult", "Adult", "Liver", "Liver and Biliary System"], [72143, "SRR22266714", "SRX18243013", "SRS15738738", "SRP407581", "PRJNA900999", "Biliary epithelial cells are facultative liver stem cells during liver regeneration in adult zebrafish", "GSE217839", "Transcriptome Analysis", "Single cell sequencing was performed on the adult zebrafish liver  on both uninjured animals  and animals regenerating from hepatocyte ablation. Overall design: Samples were sequenced at 0  1  2  3  and 7 days post ablation. Mock treated and uninjured animals were also sequenced.", null, "pubmed:36625346", null, "7 dpa  replicate 2  scRNAseq", "GSM6727458", null, "source name:liver|tissue:liver|cell type:mCherry+ cells|genotype:TgTp1:CreERT2  Tg 3.5ubb:LOXP EGFP LOXP mCherry  Tgfabp10a:CFP NTR|treatment:7 dpa", "7 dpa  replicate 2  scRNAseq", "Fastq files were processed as described using the 2.4.0 version of the Drop seq tools  mapping the reads to GRCz11 with the default parameters. For the DigitalExpression command we set NUM CORE BARCODES=5000. We used STAR version 2.5.3 and fastqc version 0.11.2. Assembly: GRCz11 Supplementary files format and content: *.txt.gz Supplementary files format and content: Digital Gene Expression Matrix", "liver", null, "Adult zebrafish livers were enyzmatically dissociated and live  nucleated cells were enriched using FACS. The resultant single cell suspension was subjected to the Drop seq protocol. Beads were subjected to reverse transcription with Maxima H Minus Reverse Transcripase. cDNA was amplified with KAPA HiFi HotStart ReadyMix. Amplified cDNA was made into libraries using the Nextera XT DNA Library Preparation Kit  with the goal of capturing 1000 cells per library. Libraries were sequenced on an Illumina NextSeq 500 using the Single End 75bp kit with the following parameters: Read 1: 20 bp  Read 2: 50 bp  Read 1 Index: 8 bp  Custom Read 1 primer  GCCTGTCCGCGGAAGCAGTGGTATCAACGCAGAGTAC.", null, "tissue:liver|cell type:mCherry+ cells|genotype:TgTp1:CreERT2  Tg 3.5ubb:LOXP EGFP LOXP mCherry  Tgfabp10a:CFP NTR|treatment:7 dpa", "GSM6727458", "GSM6727458: 7 dpa  replicate 2  scRNAseq; Danio rerio; RNA Seq", "GSM6727458 r1", "GSM6727458", "1", "Adult zebrafish livers were enyzmatically dissociated and live  nucleated cells were enriched using FACS. The resultant single cell suspension was subjected to the Drop seq protocol. Beads were subjected to reverse transcription with Maxima H Minus Reverse Transcripase. cDNA was amplified with KAPA HiFi HotStart ReadyMix. Amplified cDNA was made into libraries using the Nextera XT DNA Library Preparation Kit  with the goal of capturing 1000 cells per library. Libraries were sequenced on an Illumina NextSeq 500 using the Single End 75bp kit with the following parameters: Read 1: 20 bp  Read 2: 50 bp  Read 1 Index: 8 bp  Custom Read 1 primer  GCCTGTCCGCGGAAGCAGTGGTATCAACGCAGAGTAC.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 500", null, "SRP407581", null, null, "20200117_LIB11L_IO7719-2_S6_R1_001.fastq.gz 20200117_LIB11L_IO7719-2_S6_R2_001.fastq.gz", "fastq fastq", 3331789616.0, 45024184.0, "GSM6727458 r1", "0:25 1:49", "A:946310105;C:719718901;G:749385111;T:915418300;N:957199", 25, 49, null, null, 946310105, 719718901, 749385111, 915418300, 957199, "SRX18243013", "SRS15738738", null, null, "Division of Genetics, Brigham and Women's Hospital", 2, 0.01267, 0.86765, 0.00264, 0.03942, 0.99486, 0.90467, 0.88099, 0.69827, 25, 49, "T", "B", "sc-like readlen", "illumina", "nextseq", "unknown", "cdna_unspecified", "nextera", "sc", "single_cell_droplet", "dropseq", null, "United States", "2022-11-12", "Adult", "Adult", "Liver", "Liver and Biliary System"], [72144, "SRR22266715", "SRX18243012", "SRS15738737", "SRP407581", "PRJNA900999", "Biliary epithelial cells are facultative liver stem cells during liver regeneration in adult zebrafish", "GSE217839", "Transcriptome Analysis", "Single cell sequencing was performed on the adult zebrafish liver  on both uninjured animals  and animals regenerating from hepatocyte ablation. Overall design: Samples were sequenced at 0  1  2  3  and 7 days post ablation. Mock treated and uninjured animals were also sequenced.", null, "pubmed:36625346", null, "3 dpa  replicate 2  scRNAseq", "GSM6727457", null, "source name:liver|tissue:liver|cell type:mCherry+ cells|genotype:TgTp1:CreERT2  Tg 3.5ubb:LOXP EGFP LOXP mCherry  Tgfabp10a:CFP NTR|treatment:3 dpa", "3 dpa  replicate 2  scRNAseq", "Fastq files were processed as described using the 2.4.0 version of the Drop seq tools  mapping the reads to GRCz11 with the default parameters. For the DigitalExpression command we set NUM CORE BARCODES=5000. We used STAR version 2.5.3 and fastqc version 0.11.2. Assembly: GRCz11 Supplementary files format and content: *.txt.gz Supplementary files format and content: Digital Gene Expression Matrix", "liver", null, "Adult zebrafish livers were enyzmatically dissociated and live  nucleated cells were enriched using FACS. The resultant single cell suspension was subjected to the Drop seq protocol. Beads were subjected to reverse transcription with Maxima H Minus Reverse Transcripase. cDNA was amplified with KAPA HiFi HotStart ReadyMix. Amplified cDNA was made into libraries using the Nextera XT DNA Library Preparation Kit  with the goal of capturing 1000 cells per library. Libraries were sequenced on an Illumina NextSeq 500 using the Single End 75bp kit with the following parameters: Read 1: 20 bp  Read 2: 50 bp  Read 1 Index: 8 bp  Custom Read 1 primer  GCCTGTCCGCGGAAGCAGTGGTATCAACGCAGAGTAC.", null, "tissue:liver|cell type:mCherry+ cells|genotype:TgTp1:CreERT2  Tg 3.5ubb:LOXP EGFP LOXP mCherry  Tgfabp10a:CFP NTR|treatment:3 dpa", "GSM6727457", "GSM6727457: 3 dpa  replicate 2  scRNAseq; Danio rerio; RNA Seq", "GSM6727457 r1", "GSM6727457", "1", "Adult zebrafish livers were enyzmatically dissociated and live  nucleated cells were enriched using FACS. The resultant single cell suspension was subjected to the Drop seq protocol. Beads were subjected to reverse transcription with Maxima H Minus Reverse Transcripase. cDNA was amplified with KAPA HiFi HotStart ReadyMix. Amplified cDNA was made into libraries using the Nextera XT DNA Library Preparation Kit  with the goal of capturing 1000 cells per library. Libraries were sequenced on an Illumina NextSeq 500 using the Single End 75bp kit with the following parameters: Read 1: 20 bp  Read 2: 50 bp  Read 1 Index: 8 bp  Custom Read 1 primer  GCCTGTCCGCGGAAGCAGTGGTATCAACGCAGAGTAC.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 500", null, "SRP407581", null, null, "20200117_LIB11K_IO7719-2_S5_R1_001.fastq.gz 20200117_LIB11K_IO7719-2_S5_R2_001.fastq.gz", "fastq fastq", 3365491954.0, 45479621.0, "GSM6727457 r1", "0:25 1:49", "A:971335843;C:704444584;G:748234229;T:940472048;N:1005250", 25, 49, null, null, 971335843, 704444584, 748234229, 940472048, 1005250, "SRX18243012", "SRS15738737", null, null, "Division of Genetics, Brigham and Women's Hospital", 2, 0.01131, 0.8537, 0.00273, 0.04803, 0.99299, 0.85557, 0.84434, 0.68314, 25, 49, "T", "B", "sc-like readlen", "illumina", "nextseq", "unknown", "cdna_unspecified", "nextera", "sc", "single_cell_droplet", "dropseq", null, "United States", "2022-11-12", "Adult", "Adult", "Liver", "Liver and Biliary System"], [72145, "SRR22266716", "SRX18243011", "SRS15738736", "SRP407581", "PRJNA900999", "Biliary epithelial cells are facultative liver stem cells during liver regeneration in adult zebrafish", "GSE217839", "Transcriptome Analysis", "Single cell sequencing was performed on the adult zebrafish liver  on both uninjured animals  and animals regenerating from hepatocyte ablation. Overall design: Samples were sequenced at 0  1  2  3  and 7 days post ablation. Mock treated and uninjured animals were also sequenced.", null, "pubmed:36625346", null, "2 dpa  replicate 2  scRNAseq", "GSM6727456", null, "source name:liver|tissue:liver|cell type:mCherry+ cells|genotype:TgTp1:CreERT2  Tg 3.5ubb:LOXP EGFP LOXP mCherry  Tgfabp10a:CFP NTR|treatment:2 dpa", "2 dpa  replicate 2  scRNAseq", "Fastq files were processed as described using the 2.4.0 version of the Drop seq tools  mapping the reads to GRCz11 with the default parameters. For the DigitalExpression command we set NUM CORE BARCODES=5000. We used STAR version 2.5.3 and fastqc version 0.11.2. Assembly: GRCz11 Supplementary files format and content: *.txt.gz Supplementary files format and content: Digital Gene Expression Matrix", "liver", null, "Adult zebrafish livers were enyzmatically dissociated and live  nucleated cells were enriched using FACS. The resultant single cell suspension was subjected to the Drop seq protocol. Beads were subjected to reverse transcription with Maxima H Minus Reverse Transcripase. cDNA was amplified with KAPA HiFi HotStart ReadyMix. Amplified cDNA was made into libraries using the Nextera XT DNA Library Preparation Kit  with the goal of capturing 1000 cells per library. Libraries were sequenced on an Illumina NextSeq 500 using the Single End 75bp kit with the following parameters: Read 1: 20 bp  Read 2: 50 bp  Read 1 Index: 8 bp  Custom Read 1 primer  GCCTGTCCGCGGAAGCAGTGGTATCAACGCAGAGTAC.", null, "tissue:liver|cell type:mCherry+ cells|genotype:TgTp1:CreERT2  Tg 3.5ubb:LOXP EGFP LOXP mCherry  Tgfabp10a:CFP NTR|treatment:2 dpa", "GSM6727456", "GSM6727456: 2 dpa  replicate 2  scRNAseq; Danio rerio; RNA Seq", "GSM6727456 r1", "GSM6727456", "1", "Adult zebrafish livers were enyzmatically dissociated and live  nucleated cells were enriched using FACS. The resultant single cell suspension was subjected to the Drop seq protocol. Beads were subjected to reverse transcription with Maxima H Minus Reverse Transcripase. cDNA was amplified with KAPA HiFi HotStart ReadyMix. Amplified cDNA was made into libraries using the Nextera XT DNA Library Preparation Kit  with the goal of capturing 1000 cells per library. Libraries were sequenced on an Illumina NextSeq 500 using the Single End 75bp kit with the following parameters: Read 1: 20 bp  Read 2: 50 bp  Read 1 Index: 8 bp  Custom Read 1 primer  GCCTGTCCGCGGAAGCAGTGGTATCAACGCAGAGTAC.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 500", null, "SRP407581", null, null, "20200117_LIB11J_IO7719-2_S4_R1_001.fastq.gz 20200117_LIB11J_IO7719-2_S4_R2_001.fastq.gz", "fastq fastq", 2957589524.0, 39967426.0, "GSM6727456 r1", "0:25 1:49", "A:854274479;C:615108438;G:659483480;T:827845660;N:877467", 25, 49, null, null, 854274479, 615108438, 659483480, 827845660, 877467, "SRX18243011", "SRS15738736", null, null, "Division of Genetics, Brigham and Women's Hospital", 2, 0.01096, 0.85133, 0.00285, 0.07036, 0.99245, 0.82542, 0.82529, 0.61995, 25, 49, "T", "B", "sc-like readlen", "illumina", "nextseq", "unknown", "cdna_unspecified", "nextera", "sc", "single_cell_droplet", "dropseq", null, "United States", "2022-11-12", "Adult", "Adult", "Liver", "Liver and Biliary System"], [72146, "SRR22266717", "SRX18243010", "SRS15738735", "SRP407581", "PRJNA900999", "Biliary epithelial cells are facultative liver stem cells during liver regeneration in adult zebrafish", "GSE217839", "Transcriptome Analysis", "Single cell sequencing was performed on the adult zebrafish liver  on both uninjured animals  and animals regenerating from hepatocyte ablation. Overall design: Samples were sequenced at 0  1  2  3  and 7 days post ablation. Mock treated and uninjured animals were also sequenced.", null, "pubmed:36625346", null, "1 dpa  replicate 2  scRNAseq", "GSM6727455", null, "source name:liver|tissue:liver|cell type:mCherry+ cells|genotype:TgTp1:CreERT2  Tg 3.5ubb:LOXP EGFP LOXP mCherry  Tgfabp10a:CFP NTR|treatment:1 dpa", "1 dpa  replicate 2  scRNAseq", "Fastq files were processed as described using the 2.4.0 version of the Drop seq tools  mapping the reads to GRCz11 with the default parameters. For the DigitalExpression command we set NUM CORE BARCODES=5000. We used STAR version 2.5.3 and fastqc version 0.11.2. Assembly: GRCz11 Supplementary files format and content: *.txt.gz Supplementary files format and content: Digital Gene Expression Matrix", "liver", null, "Adult zebrafish livers were enyzmatically dissociated and live  nucleated cells were enriched using FACS. The resultant single cell suspension was subjected to the Drop seq protocol. Beads were subjected to reverse transcription with Maxima H Minus Reverse Transcripase. cDNA was amplified with KAPA HiFi HotStart ReadyMix. Amplified cDNA was made into libraries using the Nextera XT DNA Library Preparation Kit  with the goal of capturing 1000 cells per library. Libraries were sequenced on an Illumina NextSeq 500 using the Single End 75bp kit with the following parameters: Read 1: 20 bp  Read 2: 50 bp  Read 1 Index: 8 bp  Custom Read 1 primer  GCCTGTCCGCGGAAGCAGTGGTATCAACGCAGAGTAC.", null, "tissue:liver|cell type:mCherry+ cells|genotype:TgTp1:CreERT2  Tg 3.5ubb:LOXP EGFP LOXP mCherry  Tgfabp10a:CFP NTR|treatment:1 dpa", "GSM6727455", "GSM6727455: 1 dpa  replicate 2  scRNAseq; Danio rerio; RNA Seq", "GSM6727455 r1", "GSM6727455", "1", "Adult zebrafish livers were enyzmatically dissociated and live  nucleated cells were enriched using FACS. The resultant single cell suspension was subjected to the Drop seq protocol. Beads were subjected to reverse transcription with Maxima H Minus Reverse Transcripase. cDNA was amplified with KAPA HiFi HotStart ReadyMix. Amplified cDNA was made into libraries using the Nextera XT DNA Library Preparation Kit  with the goal of capturing 1000 cells per library. Libraries were sequenced on an Illumina NextSeq 500 using the Single End 75bp kit with the following parameters: Read 1: 20 bp  Read 2: 50 bp  Read 1 Index: 8 bp  Custom Read 1 primer  GCCTGTCCGCGGAAGCAGTGGTATCAACGCAGAGTAC.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 500", null, "SRP407581", null, null, "20200117_LIB11I_IO7719-2_S3_R1_001.fastq.gz 20200117_LIB11I_IO7719-2_S3_R2_001.fastq.gz", "fastq fastq", 3092505880.0, 41790620.0, "GSM6727455 r1", "0:25 1:49", "A:895995983;C:641925155;G:682159952;T:871498883;N:925907", 25, 49, null, null, 895995983, 641925155, 682159952, 871498883, 925907, "SRX18243010", "SRS15738735", null, null, "Division of Genetics, Brigham and Women's Hospital", 2, 0.01185, 0.82809, 0.00327, 0.07186, 0.99129, 0.84287, 0.7735, 0.60648, 25, 49, "T", "B", "sc-like readlen", "illumina", "nextseq", "unknown", "cdna_unspecified", "nextera", "sc", "single_cell_droplet", "dropseq", null, "United States", "2022-11-12", "Adult", "Adult", "Liver", "Liver and Biliary System"], [72147, "SRR22266718", "SRX18243009", "SRS15738734", "SRP407581", "PRJNA900999", "Biliary epithelial cells are facultative liver stem cells during liver regeneration in adult zebrafish", "GSE217839", "Transcriptome Analysis", "Single cell sequencing was performed on the adult zebrafish liver  on both uninjured animals  and animals regenerating from hepatocyte ablation. Overall design: Samples were sequenced at 0  1  2  3  and 7 days post ablation. Mock treated and uninjured animals were also sequenced.", null, "pubmed:36625346", null, "0 dpa  replicate 2  scRNAseq", "GSM6727454", null, "source name:liver|tissue:liver|cell type:mCherry+ cells|genotype:TgTp1:CreERT2  Tg 3.5ubb:LOXP EGFP LOXP mCherry  Tgfabp10a:CFP NTR|treatment:0 dpa", "0 dpa  replicate 2  scRNAseq", "Fastq files were processed as described using the 2.4.0 version of the Drop seq tools  mapping the reads to GRCz11 with the default parameters. For the DigitalExpression command we set NUM CORE BARCODES=5000. We used STAR version 2.5.3 and fastqc version 0.11.2. Assembly: GRCz11 Supplementary files format and content: *.txt.gz Supplementary files format and content: Digital Gene Expression Matrix", "liver", null, "Adult zebrafish livers were enyzmatically dissociated and live  nucleated cells were enriched using FACS. The resultant single cell suspension was subjected to the Drop seq protocol. Beads were subjected to reverse transcription with Maxima H Minus Reverse Transcripase. cDNA was amplified with KAPA HiFi HotStart ReadyMix. Amplified cDNA was made into libraries using the Nextera XT DNA Library Preparation Kit  with the goal of capturing 1000 cells per library. Libraries were sequenced on an Illumina NextSeq 500 using the Single End 75bp kit with the following parameters: Read 1: 20 bp  Read 2: 50 bp  Read 1 Index: 8 bp  Custom Read 1 primer  GCCTGTCCGCGGAAGCAGTGGTATCAACGCAGAGTAC.", null, "tissue:liver|cell type:mCherry+ cells|genotype:TgTp1:CreERT2  Tg 3.5ubb:LOXP EGFP LOXP mCherry  Tgfabp10a:CFP NTR|treatment:0 dpa", "GSM6727454", "GSM6727454: 0 dpa  replicate 2  scRNAseq; Danio rerio; RNA Seq", "GSM6727454 r1", "GSM6727454", "1", "Adult zebrafish livers were enyzmatically dissociated and live  nucleated cells were enriched using FACS. The resultant single cell suspension was subjected to the Drop seq protocol. Beads were subjected to reverse transcription with Maxima H Minus Reverse Transcripase. cDNA was amplified with KAPA HiFi HotStart ReadyMix. Amplified cDNA was made into libraries using the Nextera XT DNA Library Preparation Kit  with the goal of capturing 1000 cells per library. Libraries were sequenced on an Illumina NextSeq 500 using the Single End 75bp kit with the following parameters: Read 1: 20 bp  Read 2: 50 bp  Read 1 Index: 8 bp  Custom Read 1 primer  GCCTGTCCGCGGAAGCAGTGGTATCAACGCAGAGTAC.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 500", null, "SRP407581", null, null, "20200117_LIB11H_IO7719-2_S2_R1_001.fastq.gz 20200117_LIB11H_IO7719-2_S2_R2_001.fastq.gz", "fastq fastq", 3244996940.0, 43851310.0, "GSM6727454 r1", "0:25 1:49", "A:946884027;C:670640701;G:719365748;T:907134285;N:972179", 25, 49, null, null, 946884027, 670640701, 719365748, 907134285, 972179, "SRX18243009", "SRS15738734", null, null, "Division of Genetics, Brigham and Women's Hospital", 2, 0.01005, 0.81799, 0.00308, 0.10915, 0.99448, 0.86064, 0.79416, 0.67831, 25, 49, "T", "B", "sc-like readlen", "illumina", "nextseq", "unknown", "cdna_unspecified", "nextera", "sc", "single_cell_droplet", "dropseq", null, "United States", "2022-11-12", "Adult", "Adult", "Liver", "Liver and Biliary System"], [72148, "SRR22266719", "SRX18243008", "SRS15738733", "SRP407581", "PRJNA900999", "Biliary epithelial cells are facultative liver stem cells during liver regeneration in adult zebrafish", "GSE217839", "Transcriptome Analysis", "Single cell sequencing was performed on the adult zebrafish liver  on both uninjured animals  and animals regenerating from hepatocyte ablation. Overall design: Samples were sequenced at 0  1  2  3  and 7 days post ablation. Mock treated and uninjured animals were also sequenced.", null, "pubmed:36625346", null, "mock  replicate 2  scRNAseq", "GSM6727453", null, "source name:liver|tissue:liver|cell type:mCherry+ cells|genotype:TgTp1:CreERT2  Tg 3.5ubb:LOXP EGFP LOXP mCherry  Tgfabp10a:CFP NTR|treatment:mock", "mock  replicate 2  scRNAseq", "Fastq files were processed as described using the 2.4.0 version of the Drop seq tools  mapping the reads to GRCz11 with the default parameters. For the DigitalExpression command we set NUM CORE BARCODES=5000. We used STAR version 2.5.3 and fastqc version 0.11.2. Assembly: GRCz11 Supplementary files format and content: *.txt.gz Supplementary files format and content: Digital Gene Expression Matrix", "liver", null, "Adult zebrafish livers were enyzmatically dissociated and live  nucleated cells were enriched using FACS. The resultant single cell suspension was subjected to the Drop seq protocol. Beads were subjected to reverse transcription with Maxima H Minus Reverse Transcripase. cDNA was amplified with KAPA HiFi HotStart ReadyMix. Amplified cDNA was made into libraries using the Nextera XT DNA Library Preparation Kit  with the goal of capturing 1000 cells per library. Libraries were sequenced on an Illumina NextSeq 500 using the Single End 75bp kit with the following parameters: Read 1: 20 bp  Read 2: 50 bp  Read 1 Index: 8 bp  Custom Read 1 primer  GCCTGTCCGCGGAAGCAGTGGTATCAACGCAGAGTAC.", null, "tissue:liver|cell type:mCherry+ cells|genotype:TgTp1:CreERT2  Tg 3.5ubb:LOXP EGFP LOXP mCherry  Tgfabp10a:CFP NTR|treatment:mock", "GSM6727453", "GSM6727453: mock  replicate 2  scRNAseq; Danio rerio; RNA Seq", "GSM6727453 r1", "GSM6727453", "1", "Adult zebrafish livers were enyzmatically dissociated and live  nucleated cells were enriched using FACS. The resultant single cell suspension was subjected to the Drop seq protocol. Beads were subjected to reverse transcription with Maxima H Minus Reverse Transcripase. cDNA was amplified with KAPA HiFi HotStart ReadyMix. Amplified cDNA was made into libraries using the Nextera XT DNA Library Preparation Kit  with the goal of capturing 1000 cells per library. Libraries were sequenced on an Illumina NextSeq 500 using the Single End 75bp kit with the following parameters: Read 1: 20 bp  Read 2: 50 bp  Read 1 Index: 8 bp  Custom Read 1 primer  GCCTGTCCGCGGAAGCAGTGGTATCAACGCAGAGTAC.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 500", null, "SRP407581", null, null, "20200117_LIB11G_IO7719-2_S1_R1_001.fastq.gz 20200117_LIB11G_IO7719-2_S1_R2_001.fastq.gz", "fastq fastq", 3356731834.0, 45361241.0, "GSM6727453 r1", "0:25 1:49", "A:980697276;C:699683694;G:745214776;T:930134870;N:1001218", 25, 49, null, null, 980697276, 699683694, 745214776, 930134870, 1001218, "SRX18243008", "SRS15738733", null, null, "Division of Genetics, Brigham and Women's Hospital", 2, 0.011, 0.79428, 0.00351, 0.11936, 0.99373, 0.85478, 0.75547, 0.68815, 25, 49, "T", "B", "sc-like readlen", "illumina", "nextseq", "unknown", "cdna_unspecified", "nextera", "sc", "single_cell_droplet", "dropseq", null, "United States", "2022-11-12", "Adult", "Adult", "Liver", "Liver and Biliary System"], [72149, "SRR22266720", "SRX18243007", "SRS15738732", "SRP407581", "PRJNA900999", "Biliary epithelial cells are facultative liver stem cells during liver regeneration in adult zebrafish", "GSE217839", "Transcriptome Analysis", "Single cell sequencing was performed on the adult zebrafish liver  on both uninjured animals  and animals regenerating from hepatocyte ablation. Overall design: Samples were sequenced at 0  1  2  3  and 7 days post ablation. Mock treated and uninjured animals were also sequenced.", null, "pubmed:36625346", null, "7 dpa  replicate 1  scRNAseq", "GSM6727452", null, "source name:liver|tissue:liver|cell type:mCherry+ cells|genotype:TgTp1:CreERT2  Tg 3.5ubb:LOXP EGFP LOXP mCherry  Tgfabp10a:CFP NTR|treatment:7 dpa", "7 dpa  replicate 1  scRNAseq", "Fastq files were processed as described using the 2.4.0 version of the Drop seq tools  mapping the reads to GRCz11 with the default parameters. For the DigitalExpression command we set NUM CORE BARCODES=5000. We used STAR version 2.5.3 and fastqc version 0.11.2. Assembly: GRCz11 Supplementary files format and content: *.txt.gz Supplementary files format and content: Digital Gene Expression Matrix", "liver", null, "Adult zebrafish livers were enyzmatically dissociated and live  nucleated cells were enriched using FACS. The resultant single cell suspension was subjected to the Drop seq protocol. Beads were subjected to reverse transcription with Maxima H Minus Reverse Transcripase. cDNA was amplified with KAPA HiFi HotStart ReadyMix. Amplified cDNA was made into libraries using the Nextera XT DNA Library Preparation Kit  with the goal of capturing 1000 cells per library. Libraries were sequenced on an Illumina NextSeq 500 using the Single End 75bp kit with the following parameters: Read 1: 20 bp  Read 2: 50 bp  Read 1 Index: 8 bp  Custom Read 1 primer  GCCTGTCCGCGGAAGCAGTGGTATCAACGCAGAGTAC.", null, "tissue:liver|cell type:mCherry+ cells|genotype:TgTp1:CreERT2  Tg 3.5ubb:LOXP EGFP LOXP mCherry  Tgfabp10a:CFP NTR|treatment:7 dpa", "GSM6727452", "GSM6727452: 7 dpa  replicate 1  scRNAseq; Danio rerio; RNA Seq", "GSM6727452 r1", "GSM6727452", "1", "Adult zebrafish livers were enyzmatically dissociated and live  nucleated cells were enriched using FACS. The resultant single cell suspension was subjected to the Drop seq protocol. Beads were subjected to reverse transcription with Maxima H Minus Reverse Transcripase. cDNA was amplified with KAPA HiFi HotStart ReadyMix. Amplified cDNA was made into libraries using the Nextera XT DNA Library Preparation Kit  with the goal of capturing 1000 cells per library. Libraries were sequenced on an Illumina NextSeq 500 using the Single End 75bp kit with the following parameters: Read 1: 20 bp  Read 2: 50 bp  Read 1 Index: 8 bp  Custom Read 1 primer  GCCTGTCCGCGGAAGCAGTGGTATCAACGCAGAGTAC.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 500", null, "SRP407581", null, null, "20200107_LIB11F_IO7718_S6_R1_001.fastq.gz 20200107_LIB11F_IO7718_S6_R2_001.fastq.gz", "fastq fastq", 4840197106.0, 65408069.0, "GSM6727452 r1", "0:25 1:49", "A:1400160545;C:1045739437;G:1129539602;T:1263108590;N:1648932", 25, 49, null, null, 1400160545, 1045739437, 1129539602, 1263108590, 1648932, "SRX18243007", "SRS15738732", null, null, "Division of Genetics, Brigham and Women's Hospital", 2, 0.00433, 0.87197, 0.00121, 0.03946, 0.99849, 0.89603, 0.90123, 0.67206, 25, 49, "T", "B", "sc-like readlen", "illumina", "nextseq", "unknown", "cdna_unspecified", "nextera", "sc", "single_cell_droplet", "dropseq", null, "United States", "2022-11-12", "Adult", "Adult", "Liver", "Liver and Biliary System"], [72150, "SRR22266721", "SRX18243006", "SRS15738731", "SRP407581", "PRJNA900999", "Biliary epithelial cells are facultative liver stem cells during liver regeneration in adult zebrafish", "GSE217839", "Transcriptome Analysis", "Single cell sequencing was performed on the adult zebrafish liver  on both uninjured animals  and animals regenerating from hepatocyte ablation. Overall design: Samples were sequenced at 0  1  2  3  and 7 days post ablation. Mock treated and uninjured animals were also sequenced.", null, "pubmed:36625346", null, "3 dpa  replicate 1  scRNAseq", "GSM6727451", null, "source name:liver|tissue:liver|cell type:mCherry+ cells|genotype:TgTp1:CreERT2  Tg 3.5ubb:LOXP EGFP LOXP mCherry  Tgfabp10a:CFP NTR|treatment:3 dpa", "3 dpa  replicate 1  scRNAseq", "Fastq files were processed as described using the 2.4.0 version of the Drop seq tools  mapping the reads to GRCz11 with the default parameters. For the DigitalExpression command we set NUM CORE BARCODES=5000. We used STAR version 2.5.3 and fastqc version 0.11.2. Assembly: GRCz11 Supplementary files format and content: *.txt.gz Supplementary files format and content: Digital Gene Expression Matrix", "liver", null, "Adult zebrafish livers were enyzmatically dissociated and live  nucleated cells were enriched using FACS. The resultant single cell suspension was subjected to the Drop seq protocol. Beads were subjected to reverse transcription with Maxima H Minus Reverse Transcripase. cDNA was amplified with KAPA HiFi HotStart ReadyMix. Amplified cDNA was made into libraries using the Nextera XT DNA Library Preparation Kit  with the goal of capturing 1000 cells per library. Libraries were sequenced on an Illumina NextSeq 500 using the Single End 75bp kit with the following parameters: Read 1: 20 bp  Read 2: 50 bp  Read 1 Index: 8 bp  Custom Read 1 primer  GCCTGTCCGCGGAAGCAGTGGTATCAACGCAGAGTAC.", null, "tissue:liver|cell type:mCherry+ cells|genotype:TgTp1:CreERT2  Tg 3.5ubb:LOXP EGFP LOXP mCherry  Tgfabp10a:CFP NTR|treatment:3 dpa", "GSM6727451", "GSM6727451: 3 dpa  replicate 1  scRNAseq; Danio rerio; RNA Seq", "GSM6727451 r1", "GSM6727451", "1", "Adult zebrafish livers were enyzmatically dissociated and live  nucleated cells were enriched using FACS. The resultant single cell suspension was subjected to the Drop seq protocol. Beads were subjected to reverse transcription with Maxima H Minus Reverse Transcripase. cDNA was amplified with KAPA HiFi HotStart ReadyMix. Amplified cDNA was made into libraries using the Nextera XT DNA Library Preparation Kit  with the goal of capturing 1000 cells per library. Libraries were sequenced on an Illumina NextSeq 500 using the Single End 75bp kit with the following parameters: Read 1: 20 bp  Read 2: 50 bp  Read 1 Index: 8 bp  Custom Read 1 primer  GCCTGTCCGCGGAAGCAGTGGTATCAACGCAGAGTAC.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 500", null, "SRP407581", null, null, "20200107_LIB11E_IO7718_S5_R1_001.fastq.gz 20200107_LIB11E_IO7718_S5_R2_001.fastq.gz", "fastq fastq", 3874899962.0, 52363513.0, "GSM6727451 r1", "0:25 1:49", "A:1146498594;C:801586968;G:878214009;T:1047253303;N:1347088", 25, 49, null, null, 1146498594, 801586968, 878214009, 1047253303, 1347088, "SRX18243006", "SRS15738731", null, null, "Division of Genetics, Brigham and Women's Hospital", 2, 0.00431, 0.83478, 0.00141, 0.0513, 0.99803, 0.84839, 0.89787, 0.68748, 25, 49, "T", "B", "sc-like readlen", "illumina", "nextseq", "unknown", "cdna_unspecified", "nextera", "sc", "single_cell_droplet", "dropseq", null, "United States", "2022-11-12", "Adult", "Adult", "Liver", "Liver and Biliary System"], [72151, "SRR22266722", "SRX18243005", "SRS15738730", "SRP407581", "PRJNA900999", "Biliary epithelial cells are facultative liver stem cells during liver regeneration in adult zebrafish", "GSE217839", "Transcriptome Analysis", "Single cell sequencing was performed on the adult zebrafish liver  on both uninjured animals  and animals regenerating from hepatocyte ablation. Overall design: Samples were sequenced at 0  1  2  3  and 7 days post ablation. Mock treated and uninjured animals were also sequenced.", null, "pubmed:36625346", null, "2 dpa  replicate 1  scRNAseq", "GSM6727450", null, "source name:liver|tissue:liver|cell type:mCherry+ cells|genotype:TgTp1:CreERT2  Tg 3.5ubb:LOXP EGFP LOXP mCherry  Tgfabp10a:CFP NTR|treatment:2 dpa", "2 dpa  replicate 1  scRNAseq", "Fastq files were processed as described using the 2.4.0 version of the Drop seq tools  mapping the reads to GRCz11 with the default parameters. For the DigitalExpression command we set NUM CORE BARCODES=5000. We used STAR version 2.5.3 and fastqc version 0.11.2. Assembly: GRCz11 Supplementary files format and content: *.txt.gz Supplementary files format and content: Digital Gene Expression Matrix", "liver", null, "Adult zebrafish livers were enyzmatically dissociated and live  nucleated cells were enriched using FACS. The resultant single cell suspension was subjected to the Drop seq protocol. Beads were subjected to reverse transcription with Maxima H Minus Reverse Transcripase. cDNA was amplified with KAPA HiFi HotStart ReadyMix. Amplified cDNA was made into libraries using the Nextera XT DNA Library Preparation Kit  with the goal of capturing 1000 cells per library. Libraries were sequenced on an Illumina NextSeq 500 using the Single End 75bp kit with the following parameters: Read 1: 20 bp  Read 2: 50 bp  Read 1 Index: 8 bp  Custom Read 1 primer  GCCTGTCCGCGGAAGCAGTGGTATCAACGCAGAGTAC.", null, "tissue:liver|cell type:mCherry+ cells|genotype:TgTp1:CreERT2  Tg 3.5ubb:LOXP EGFP LOXP mCherry  Tgfabp10a:CFP NTR|treatment:2 dpa", "GSM6727450", "GSM6727450: 2 dpa  replicate 1  scRNAseq; Danio rerio; RNA Seq", "GSM6727450 r1", "GSM6727450", "1", "Adult zebrafish livers were enyzmatically dissociated and live  nucleated cells were enriched using FACS. The resultant single cell suspension was subjected to the Drop seq protocol. Beads were subjected to reverse transcription with Maxima H Minus Reverse Transcripase. cDNA was amplified with KAPA HiFi HotStart ReadyMix. Amplified cDNA was made into libraries using the Nextera XT DNA Library Preparation Kit  with the goal of capturing 1000 cells per library. Libraries were sequenced on an Illumina NextSeq 500 using the Single End 75bp kit with the following parameters: Read 1: 20 bp  Read 2: 50 bp  Read 1 Index: 8 bp  Custom Read 1 primer  GCCTGTCCGCGGAAGCAGTGGTATCAACGCAGAGTAC.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 500", null, "SRP407581", null, null, "20200107_LIB11D_IO7718_S4_R1_001.fastq.gz 20200107_LIB11D_IO7718_S4_R2_001.fastq.gz", "fastq fastq", 4290500982.0, 57979743.0, "GSM6727450 r1", "0:25 1:49", "A:1253875889;C:893179946;G:979642333;T:1162325902;N:1476912", 25, 49, null, null, 1253875889, 893179946, 979642333, 1162325902, 1476912, "SRX18243005", "SRS15738730", null, null, "Division of Genetics, Brigham and Women's Hospital", 2, 0.00318, 0.85906, 0.00119, 0.07192, 0.99791, 0.81201, 0.87066, 0.6047, 25, 49, "T", "B", "sc-like readlen", "illumina", "nextseq", "unknown", "cdna_unspecified", "nextera", "sc", "single_cell_droplet", "dropseq", null, "United States", "2022-11-12", "Adult", "Adult", "Liver", "Liver and Biliary System"], [72152, "SRR22266723", "SRX18243004", "SRS15738729", "SRP407581", "PRJNA900999", "Biliary epithelial cells are facultative liver stem cells during liver regeneration in adult zebrafish", "GSE217839", "Transcriptome Analysis", "Single cell sequencing was performed on the adult zebrafish liver  on both uninjured animals  and animals regenerating from hepatocyte ablation. Overall design: Samples were sequenced at 0  1  2  3  and 7 days post ablation. Mock treated and uninjured animals were also sequenced.", null, "pubmed:36625346", null, "1 dpa  replicate 1  scRNAseq", "GSM6727449", null, "source name:liver|tissue:liver|cell type:mCherry+ cells|genotype:TgTp1:CreERT2  Tg 3.5ubb:LOXP EGFP LOXP mCherry  Tgfabp10a:CFP NTR|treatment:1 dpa", "1 dpa  replicate 1  scRNAseq", "Fastq files were processed as described using the 2.4.0 version of the Drop seq tools  mapping the reads to GRCz11 with the default parameters. For the DigitalExpression command we set NUM CORE BARCODES=5000. We used STAR version 2.5.3 and fastqc version 0.11.2. Assembly: GRCz11 Supplementary files format and content: *.txt.gz Supplementary files format and content: Digital Gene Expression Matrix", "liver", null, "Adult zebrafish livers were enyzmatically dissociated and live  nucleated cells were enriched using FACS. The resultant single cell suspension was subjected to the Drop seq protocol. Beads were subjected to reverse transcription with Maxima H Minus Reverse Transcripase. cDNA was amplified with KAPA HiFi HotStart ReadyMix. Amplified cDNA was made into libraries using the Nextera XT DNA Library Preparation Kit  with the goal of capturing 1000 cells per library. Libraries were sequenced on an Illumina NextSeq 500 using the Single End 75bp kit with the following parameters: Read 1: 20 bp  Read 2: 50 bp  Read 1 Index: 8 bp  Custom Read 1 primer  GCCTGTCCGCGGAAGCAGTGGTATCAACGCAGAGTAC.", null, "tissue:liver|cell type:mCherry+ cells|genotype:TgTp1:CreERT2  Tg 3.5ubb:LOXP EGFP LOXP mCherry  Tgfabp10a:CFP NTR|treatment:1 dpa", "GSM6727449", "GSM6727449: 1 dpa  replicate 1  scRNAseq; Danio rerio; RNA Seq", "GSM6727449 r1", "GSM6727449", "1", "Adult zebrafish livers were enyzmatically dissociated and live  nucleated cells were enriched using FACS. The resultant single cell suspension was subjected to the Drop seq protocol. Beads were subjected to reverse transcription with Maxima H Minus Reverse Transcripase. cDNA was amplified with KAPA HiFi HotStart ReadyMix. Amplified cDNA was made into libraries using the Nextera XT DNA Library Preparation Kit  with the goal of capturing 1000 cells per library. Libraries were sequenced on an Illumina NextSeq 500 using the Single End 75bp kit with the following parameters: Read 1: 20 bp  Read 2: 50 bp  Read 1 Index: 8 bp  Custom Read 1 primer  GCCTGTCCGCGGAAGCAGTGGTATCAACGCAGAGTAC.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 500", null, "SRP407581", null, null, "20200107_LIB11C_IO7718_S3_R1_001.fastq.gz 20200107_LIB11C_IO7718_S3_R2_001.fastq.gz", "fastq fastq", 4132383258.0, 55843017.0, "GSM6727449 r1", "0:25 1:49", "A:1190680505;C:866874473;G:962110856;T:1111280875;N:1436549", 25, 49, null, null, 1190680505, 866874473, 962110856, 1111280875, 1436549, "SRX18243004", "SRS15738729", null, null, "Division of Genetics, Brigham and Women's Hospital", 2, 0.00379, 0.84562, 0.00142, 0.07321, 0.99793, 0.82974, 0.87967, 0.60001, 25, 49, "T", "B", "sc-like readlen", "illumina", "nextseq", "unknown", "cdna_unspecified", "nextera", "sc", "single_cell_droplet", "dropseq", null, "United States", "2022-11-12", "Adult", "Adult", "Liver", "Liver and Biliary System"], [72153, "SRR22266724", "SRX18243003", "SRS15738728", "SRP407581", "PRJNA900999", "Biliary epithelial cells are facultative liver stem cells during liver regeneration in adult zebrafish", "GSE217839", "Transcriptome Analysis", "Single cell sequencing was performed on the adult zebrafish liver  on both uninjured animals  and animals regenerating from hepatocyte ablation. Overall design: Samples were sequenced at 0  1  2  3  and 7 days post ablation. Mock treated and uninjured animals were also sequenced.", null, "pubmed:36625346", null, "0 dpa  replicate 1  scRNAseq", "GSM6727448", null, "source name:liver|tissue:liver|cell type:mCherry+ cells|genotype:TgTp1:CreERT2  Tg 3.5ubb:LOXP EGFP LOXP mCherry  Tgfabp10a:CFP NTR|treatment:0 dpa", "0 dpa  replicate 1  scRNAseq", "Fastq files were processed as described using the 2.4.0 version of the Drop seq tools  mapping the reads to GRCz11 with the default parameters. For the DigitalExpression command we set NUM CORE BARCODES=5000. We used STAR version 2.5.3 and fastqc version 0.11.2. Assembly: GRCz11 Supplementary files format and content: *.txt.gz Supplementary files format and content: Digital Gene Expression Matrix", "liver", null, "Adult zebrafish livers were enyzmatically dissociated and live  nucleated cells were enriched using FACS. The resultant single cell suspension was subjected to the Drop seq protocol. Beads were subjected to reverse transcription with Maxima H Minus Reverse Transcripase. cDNA was amplified with KAPA HiFi HotStart ReadyMix. Amplified cDNA was made into libraries using the Nextera XT DNA Library Preparation Kit  with the goal of capturing 1000 cells per library. Libraries were sequenced on an Illumina NextSeq 500 using the Single End 75bp kit with the following parameters: Read 1: 20 bp  Read 2: 50 bp  Read 1 Index: 8 bp  Custom Read 1 primer  GCCTGTCCGCGGAAGCAGTGGTATCAACGCAGAGTAC.", null, "tissue:liver|cell type:mCherry+ cells|genotype:TgTp1:CreERT2  Tg 3.5ubb:LOXP EGFP LOXP mCherry  Tgfabp10a:CFP NTR|treatment:0 dpa", "GSM6727448", "GSM6727448: 0 dpa  replicate 1  scRNAseq; Danio rerio; RNA Seq", "GSM6727448 r1", "GSM6727448", "1", "Adult zebrafish livers were enyzmatically dissociated and live  nucleated cells were enriched using FACS. The resultant single cell suspension was subjected to the Drop seq protocol. Beads were subjected to reverse transcription with Maxima H Minus Reverse Transcripase. cDNA was amplified with KAPA HiFi HotStart ReadyMix. Amplified cDNA was made into libraries using the Nextera XT DNA Library Preparation Kit  with the goal of capturing 1000 cells per library. Libraries were sequenced on an Illumina NextSeq 500 using the Single End 75bp kit with the following parameters: Read 1: 20 bp  Read 2: 50 bp  Read 1 Index: 8 bp  Custom Read 1 primer  GCCTGTCCGCGGAAGCAGTGGTATCAACGCAGAGTAC.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 500", null, "SRP407581", null, null, "20200107_LIB11B_IO7718_S2_R1_001.fastq.gz 20200107_LIB11B_IO7718_S2_R2_001.fastq.gz", "fastq fastq", 3517854180.0, 47538570.0, "GSM6727448 r1", "0:25 1:49", "A:1048135042;C:723233290;G:804794008;T:940454450;N:1237390", 25, 49, null, null, 1048135042, 723233290, 804794008, 940454450, 1237390, "SRX18243003", "SRS15738728", null, null, "Division of Genetics, Brigham and Women's Hospital", 2, 0.00348, 0.83158, 0.00149, 0.11394, 0.99817, 0.84737, 0.77258, 0.68387, 25, 49, "T", "B", "sc-like readlen", "illumina", "nextseq", "unknown", "cdna_unspecified", "nextera", "sc", "single_cell_droplet", "dropseq", null, "United States", "2022-11-12", "Adult", "Adult", "Liver", "Liver and Biliary System"], [72154, "SRR22266725", "SRX18243002", "SRS15738727", "SRP407581", "PRJNA900999", "Biliary epithelial cells are facultative liver stem cells during liver regeneration in adult zebrafish", "GSE217839", "Transcriptome Analysis", "Single cell sequencing was performed on the adult zebrafish liver  on both uninjured animals  and animals regenerating from hepatocyte ablation. Overall design: Samples were sequenced at 0  1  2  3  and 7 days post ablation. Mock treated and uninjured animals were also sequenced.", null, "pubmed:36625346", null, "mock  replicate 1  scRNAseq", "GSM6727447", null, "source name:liver|tissue:liver|cell type:mCherry+ cells|genotype:TgTp1:CreERT2  Tg 3.5ubb:LOXP EGFP LOXP mCherry  Tgfabp10a:CFP NTR|treatment:mock", "mock  replicate 1  scRNAseq", "Fastq files were processed as described using the 2.4.0 version of the Drop seq tools  mapping the reads to GRCz11 with the default parameters. For the DigitalExpression command we set NUM CORE BARCODES=5000. We used STAR version 2.5.3 and fastqc version 0.11.2. Assembly: GRCz11 Supplementary files format and content: *.txt.gz Supplementary files format and content: Digital Gene Expression Matrix", "liver", null, "Adult zebrafish livers were enyzmatically dissociated and live  nucleated cells were enriched using FACS. The resultant single cell suspension was subjected to the Drop seq protocol. Beads were subjected to reverse transcription with Maxima H Minus Reverse Transcripase. cDNA was amplified with KAPA HiFi HotStart ReadyMix. Amplified cDNA was made into libraries using the Nextera XT DNA Library Preparation Kit  with the goal of capturing 1000 cells per library. Libraries were sequenced on an Illumina NextSeq 500 using the Single End 75bp kit with the following parameters: Read 1: 20 bp  Read 2: 50 bp  Read 1 Index: 8 bp  Custom Read 1 primer  GCCTGTCCGCGGAAGCAGTGGTATCAACGCAGAGTAC.", null, "tissue:liver|cell type:mCherry+ cells|genotype:TgTp1:CreERT2  Tg 3.5ubb:LOXP EGFP LOXP mCherry  Tgfabp10a:CFP NTR|treatment:mock", "GSM6727447", "GSM6727447: mock  replicate 1  scRNAseq; Danio rerio; RNA Seq", "GSM6727447 r1", "GSM6727447", "1", "Adult zebrafish livers were enyzmatically dissociated and live  nucleated cells were enriched using FACS. The resultant single cell suspension was subjected to the Drop seq protocol. Beads were subjected to reverse transcription with Maxima H Minus Reverse Transcripase. cDNA was amplified with KAPA HiFi HotStart ReadyMix. Amplified cDNA was made into libraries using the Nextera XT DNA Library Preparation Kit  with the goal of capturing 1000 cells per library. Libraries were sequenced on an Illumina NextSeq 500 using the Single End 75bp kit with the following parameters: Read 1: 20 bp  Read 2: 50 bp  Read 1 Index: 8 bp  Custom Read 1 primer  GCCTGTCCGCGGAAGCAGTGGTATCAACGCAGAGTAC.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 500", null, "SRP407581", null, null, "20200107_LIB11A_IO7718_S1_R1_001.fastq.gz 20200107_LIB11A_IO7718_S1_R2_001.fastq.gz", "fastq fastq", 3054768766.0, 41280659.0, "GSM6727447 r1", "0:25 1:49", "A:927118702;C:625458757;G:696040428;T:805077948;N:1072931", 25, 49, null, null, 927118702, 625458757, 696040428, 805077948, 1072931, "SRX18243002", "SRS15738727", null, null, "Division of Genetics, Brigham and Women's Hospital", 2, 0.00329, 0.78939, 0.00143, 0.12148, 0.99819, 0.84214, 0.82711, 0.67658, 25, 49, "T", "B", "sc-like readlen", "illumina", "nextseq", "unknown", "cdna_unspecified", "nextera", "sc", "single_cell_droplet", "dropseq", null, "United States", "2022-11-12", "Adult", "Adult", "Liver", "Liver and Biliary System"], [72155, "SRR22266726", "SRX18243001", "SRS15738726", "SRP407581", "PRJNA900999", "Biliary epithelial cells are facultative liver stem cells during liver regeneration in adult zebrafish", "GSE217839", "Transcriptome Analysis", "Single cell sequencing was performed on the adult zebrafish liver  on both uninjured animals  and animals regenerating from hepatocyte ablation. Overall design: Samples were sequenced at 0  1  2  3  and 7 days post ablation. Mock treated and uninjured animals were also sequenced.", null, "pubmed:36625346", null, "uninjured GFP+ cells  replicate 1  scRNAseq", "GSM6727446", null, "source name:liver|tissue:liver|cell type:GFP+ cells|genotype:TgTp1:CreERT2  Tg 3.5ubb:LOXP EGFP LOXP mCherry  Tgfabp10a:CFP NTR|treatment:Uninjured", "uninjured GFP+ cells  replicate 1  scRNAseq", "Fastq files were processed as described using the 2.4.0 version of the Drop seq tools  mapping the reads to GRCz11 with the default parameters. For the DigitalExpression command we set NUM CORE BARCODES=5000. We used STAR version 2.5.3 and fastqc version 0.11.2. Assembly: GRCz11 Supplementary files format and content: *.txt.gz Supplementary files format and content: Digital Gene Expression Matrix", "liver", null, "Adult zebrafish livers were enyzmatically dissociated and live  nucleated cells were enriched using FACS. The resultant single cell suspension was subjected to the Drop seq protocol. Beads were subjected to reverse transcription with Maxima H Minus Reverse Transcripase. cDNA was amplified with KAPA HiFi HotStart ReadyMix. Amplified cDNA was made into libraries using the Nextera XT DNA Library Preparation Kit  with the goal of capturing 1000 cells per library. Libraries were sequenced on an Illumina NextSeq 500 using the Single End 75bp kit with the following parameters: Read 1: 20 bp  Read 2: 50 bp  Read 1 Index: 8 bp  Custom Read 1 primer  GCCTGTCCGCGGAAGCAGTGGTATCAACGCAGAGTAC.", null, "tissue:liver|cell type:GFP+ cells|genotype:TgTp1:CreERT2  Tg 3.5ubb:LOXP EGFP LOXP mCherry  Tgfabp10a:CFP NTR|treatment:Uninjured", "GSM6727446", "GSM6727446: uninjured GFP+ cells  replicate 1  scRNAseq; Danio rerio; RNA Seq", "GSM6727446 r1", "GSM6727446", "1", "Adult zebrafish livers were enyzmatically dissociated and live  nucleated cells were enriched using FACS. The resultant single cell suspension was subjected to the Drop seq protocol. Beads were subjected to reverse transcription with Maxima H Minus Reverse Transcripase. cDNA was amplified with KAPA HiFi HotStart ReadyMix. Amplified cDNA was made into libraries using the Nextera XT DNA Library Preparation Kit  with the goal of capturing 1000 cells per library. Libraries were sequenced on an Illumina NextSeq 500 using the Single End 75bp kit with the following parameters: Read 1: 20 bp  Read 2: 50 bp  Read 1 Index: 8 bp  Custom Read 1 primer  GCCTGTCCGCGGAAGCAGTGGTATCAACGCAGAGTAC.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 500", null, "SRP407581", null, null, "20191106_LIB10D_IO7549_S4_R1_001.fastq.gz 20191106_LIB10D_IO7549_S4_R2_001.fastq.gz", "fastq fastq", 8587107112.0, 116041988.0, "GSM6727446 r1", "0:25 1:49", "A:2580895720;C:1753463233;G:1921984995;T:2327869497;N:2893667", 25, 49, null, null, 2580895720, 1753463233, 1921984995, 2327869497, 2893667, "SRX18243001", "SRS15738726", null, null, "Division of Genetics, Brigham and Women's Hospital", 2, 0.02318, 0.81602, 0.00376, 0.07042, 0.99464, 0.925, 0.94019, 0.87362, 25, 49, "T", "B", "sc-like readlen", "illumina", "nextseq", "unknown", "cdna_unspecified", "nextera", "sc", "single_cell_droplet", "dropseq", null, "United States", "2022-11-12", "Adult", "Adult", "Liver", "Liver and Biliary System"], [72156, "SRR22266727", "SRX18243000", "SRS15738725", "SRP407581", "PRJNA900999", "Biliary epithelial cells are facultative liver stem cells during liver regeneration in adult zebrafish", "GSE217839", "Transcriptome Analysis", "Single cell sequencing was performed on the adult zebrafish liver  on both uninjured animals  and animals regenerating from hepatocyte ablation. Overall design: Samples were sequenced at 0  1  2  3  and 7 days post ablation. Mock treated and uninjured animals were also sequenced.", null, "pubmed:36625346", null, "uninjured mCherry+ cells  replicate 3  scRNAseq", "GSM6727445", null, "source name:liver|tissue:liver|cell type:mCherry+ cells|genotype:TgTp1:CreERT2  Tg 3.5ubb:LOXP EGFP LOXP mCherry  Tgfabp10a:CFP NTR|treatment:Uninjured", "uninjured mCherry+ cells  replicate 3  scRNAseq", "Fastq files were processed as described using the 2.4.0 version of the Drop seq tools  mapping the reads to GRCz11 with the default parameters. For the DigitalExpression command we set NUM CORE BARCODES=5000. We used STAR version 2.5.3 and fastqc version 0.11.2. Assembly: GRCz11 Supplementary files format and content: *.txt.gz Supplementary files format and content: Digital Gene Expression Matrix", "liver", null, "Adult zebrafish livers were enyzmatically dissociated and live  nucleated cells were enriched using FACS. The resultant single cell suspension was subjected to the Drop seq protocol. Beads were subjected to reverse transcription with Maxima H Minus Reverse Transcripase. cDNA was amplified with KAPA HiFi HotStart ReadyMix. Amplified cDNA was made into libraries using the Nextera XT DNA Library Preparation Kit  with the goal of capturing 1000 cells per library. Libraries were sequenced on an Illumina NextSeq 500 using the Single End 75bp kit with the following parameters: Read 1: 20 bp  Read 2: 50 bp  Read 1 Index: 8 bp  Custom Read 1 primer  GCCTGTCCGCGGAAGCAGTGGTATCAACGCAGAGTAC.", null, "tissue:liver|cell type:mCherry+ cells|genotype:TgTp1:CreERT2  Tg 3.5ubb:LOXP EGFP LOXP mCherry  Tgfabp10a:CFP NTR|treatment:Uninjured", "GSM6727445", "GSM6727445: uninjured mCherry+ cells  replicate 3  scRNAseq; Danio rerio; RNA Seq", "GSM6727445 r1", "GSM6727445", "1", "Adult zebrafish livers were enyzmatically dissociated and live  nucleated cells were enriched using FACS. The resultant single cell suspension was subjected to the Drop seq protocol. Beads were subjected to reverse transcription with Maxima H Minus Reverse Transcripase. cDNA was amplified with KAPA HiFi HotStart ReadyMix. Amplified cDNA was made into libraries using the Nextera XT DNA Library Preparation Kit  with the goal of capturing 1000 cells per library. Libraries were sequenced on an Illumina NextSeq 500 using the Single End 75bp kit with the following parameters: Read 1: 20 bp  Read 2: 50 bp  Read 1 Index: 8 bp  Custom Read 1 primer  GCCTGTCCGCGGAAGCAGTGGTATCAACGCAGAGTAC.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 500", null, "SRP407581", null, null, "20191106_LIB10C_IO7549_S3_R1_001.fastq.gz 20191106_LIB10C_IO7549_S3_R2_001.fastq.gz", "fastq fastq", 8043400252.0, 108694598.0, "GSM6727445 r1", "0:25 1:49", "A:2306379322;C:1670142146;G:1834433742;T:2229698464;N:2746578", 25, 49, null, null, 2306379322, 1670142146, 1834433742, 2229698464, 2746578, "SRX18243000", "SRS15738725", null, null, "Division of Genetics, Brigham and Women's Hospital", 2, 0.02105, 0.77629, 0.00529, 0.12681, 0.98827, 0.84358, 0.77585, 0.67675, 25, 49, "T", "B", "sc-like readlen", "illumina", "nextseq", "unknown", "cdna_unspecified", "nextera", "sc", "single_cell_droplet", "dropseq", null, "United States", "2022-11-12", "Adult", "Adult", "Liver", "Liver and Biliary System"], [72157, "SRR22266728", "SRX18242999", "SRS15738724", "SRP407581", "PRJNA900999", "Biliary epithelial cells are facultative liver stem cells during liver regeneration in adult zebrafish", "GSE217839", "Transcriptome Analysis", "Single cell sequencing was performed on the adult zebrafish liver  on both uninjured animals  and animals regenerating from hepatocyte ablation. Overall design: Samples were sequenced at 0  1  2  3  and 7 days post ablation. Mock treated and uninjured animals were also sequenced.", null, "pubmed:36625346", null, "uninjured mCherry+ cells  replicate 2  scRNAseq", "GSM6727444", null, "source name:liver|tissue:liver|cell type:mCherry+ cells|genotype:TgTp1:CreERT2  Tg 3.5ubb:LOXP EGFP LOXP mCherry  Tgfabp10a:CFP NTR|treatment:Uninjured", "uninjured mCherry+ cells  replicate 2  scRNAseq", "Fastq files were processed as described using the 2.4.0 version of the Drop seq tools  mapping the reads to GRCz11 with the default parameters. For the DigitalExpression command we set NUM CORE BARCODES=5000. We used STAR version 2.5.3 and fastqc version 0.11.2. Assembly: GRCz11 Supplementary files format and content: *.txt.gz Supplementary files format and content: Digital Gene Expression Matrix", "liver", null, "Adult zebrafish livers were enyzmatically dissociated and live  nucleated cells were enriched using FACS. The resultant single cell suspension was subjected to the Drop seq protocol. Beads were subjected to reverse transcription with Maxima H Minus Reverse Transcripase. cDNA was amplified with KAPA HiFi HotStart ReadyMix. Amplified cDNA was made into libraries using the Nextera XT DNA Library Preparation Kit  with the goal of capturing 1000 cells per library. Libraries were sequenced on an Illumina NextSeq 500 using the Single End 75bp kit with the following parameters: Read 1: 20 bp  Read 2: 50 bp  Read 1 Index: 8 bp  Custom Read 1 primer  GCCTGTCCGCGGAAGCAGTGGTATCAACGCAGAGTAC.", null, "tissue:liver|cell type:mCherry+ cells|genotype:TgTp1:CreERT2  Tg 3.5ubb:LOXP EGFP LOXP mCherry  Tgfabp10a:CFP NTR|treatment:Uninjured", "GSM6727444", "GSM6727444: uninjured mCherry+ cells  replicate 2  scRNAseq; Danio rerio; RNA Seq", "GSM6727444 r1", "GSM6727444", "1", "Adult zebrafish livers were enyzmatically dissociated and live  nucleated cells were enriched using FACS. The resultant single cell suspension was subjected to the Drop seq protocol. Beads were subjected to reverse transcription with Maxima H Minus Reverse Transcripase. cDNA was amplified with KAPA HiFi HotStart ReadyMix. Amplified cDNA was made into libraries using the Nextera XT DNA Library Preparation Kit  with the goal of capturing 1000 cells per library. Libraries were sequenced on an Illumina NextSeq 500 using the Single End 75bp kit with the following parameters: Read 1: 20 bp  Read 2: 50 bp  Read 1 Index: 8 bp  Custom Read 1 primer  GCCTGTCCGCGGAAGCAGTGGTATCAACGCAGAGTAC.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 500", null, "SRP407581", null, null, "20191106_LIB10B_IO7549_S2_R1_001.fastq.gz 20191106_LIB10B_IO7549_S2_R2_001.fastq.gz", "fastq fastq", 7485031772.0, 101149078.0, "GSM6727444 r1", "0:25 1:49", "A:2109751380;C:1556629400;G:1728261758;T:2087832467;N:2556767", 25, 49, null, null, 2109751380, 1556629400, 1728261758, 2087832467, 2556767, "SRX18242999", "SRS15738724", null, null, "Division of Genetics, Brigham and Women's Hospital", 2, 0.02151, 0.80489, 0.00493, 0.13325, 0.9893, 0.84776, 0.77861, 0.68035, 25, 49, "T", "B", "sc-like readlen", "illumina", "nextseq", "unknown", "cdna_unspecified", "nextera", "sc", "single_cell_droplet", "dropseq", null, "United States", "2022-11-12", "Adult", "Adult", "Liver", "Liver and Biliary System"], [72158, "SRR22266729", "SRX18242998", "SRS15738723", "SRP407581", "PRJNA900999", "Biliary epithelial cells are facultative liver stem cells during liver regeneration in adult zebrafish", "GSE217839", "Transcriptome Analysis", "Single cell sequencing was performed on the adult zebrafish liver  on both uninjured animals  and animals regenerating from hepatocyte ablation. Overall design: Samples were sequenced at 0  1  2  3  and 7 days post ablation. Mock treated and uninjured animals were also sequenced.", null, "pubmed:36625346", null, "uninjured mCherry+ cells  replicate 1  scRNAseq", "GSM6727443", null, "source name:liver|tissue:liver|cell type:mCherry+ cells|genotype:TgTp1:CreERT2  Tg 3.5ubb:LOXP EGFP LOXP mCherry  Tgfabp10a:CFP NTR|treatment:Uninjured", "uninjured mCherry+ cells  replicate 1  scRNAseq", "Fastq files were processed as described using the 2.4.0 version of the Drop seq tools  mapping the reads to GRCz11 with the default parameters. For the DigitalExpression command we set NUM CORE BARCODES=5000. We used STAR version 2.5.3 and fastqc version 0.11.2. Assembly: GRCz11 Supplementary files format and content: *.txt.gz Supplementary files format and content: Digital Gene Expression Matrix", "liver", null, "Adult zebrafish livers were enyzmatically dissociated and live  nucleated cells were enriched using FACS. The resultant single cell suspension was subjected to the Drop seq protocol. Beads were subjected to reverse transcription with Maxima H Minus Reverse Transcripase. cDNA was amplified with KAPA HiFi HotStart ReadyMix. Amplified cDNA was made into libraries using the Nextera XT DNA Library Preparation Kit  with the goal of capturing 1000 cells per library. Libraries were sequenced on an Illumina NextSeq 500 using the Single End 75bp kit with the following parameters: Read 1: 20 bp  Read 2: 50 bp  Read 1 Index: 8 bp  Custom Read 1 primer  GCCTGTCCGCGGAAGCAGTGGTATCAACGCAGAGTAC.", null, "tissue:liver|cell type:mCherry+ cells|genotype:TgTp1:CreERT2  Tg 3.5ubb:LOXP EGFP LOXP mCherry  Tgfabp10a:CFP NTR|treatment:Uninjured", "GSM6727443", "GSM6727443: uninjured mCherry+ cells  replicate 1  scRNAseq; Danio rerio; RNA Seq", "GSM6727443 r1", "GSM6727443", "1", "Adult zebrafish livers were enyzmatically dissociated and live  nucleated cells were enriched using FACS. The resultant single cell suspension was subjected to the Drop seq protocol. Beads were subjected to reverse transcription with Maxima H Minus Reverse Transcripase. cDNA was amplified with KAPA HiFi HotStart ReadyMix. Amplified cDNA was made into libraries using the Nextera XT DNA Library Preparation Kit  with the goal of capturing 1000 cells per library. Libraries were sequenced on an Illumina NextSeq 500 using the Single End 75bp kit with the following parameters: Read 1: 20 bp  Read 2: 50 bp  Read 1 Index: 8 bp  Custom Read 1 primer  GCCTGTCCGCGGAAGCAGTGGTATCAACGCAGAGTAC.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 500", null, "SRP407581", null, null, "20191106_LIB10A_IO7549_S1_R1_001.fastq.gz 20191106_LIB10A_IO7549_S1_R2_001.fastq.gz", "fastq fastq", 9329321266.0, 126071909.0, "GSM6727443 r1", "0:25 1:49", "A:2676820500;C:1936185283;G:2114138905;T:2598981203;N:3195375", 25, 49, null, null, 2676820500, 1936185283, 2114138905, 2598981203, 3195375, "SRX18242998", "SRS15738723", null, null, "Division of Genetics, Brigham and Women's Hospital", 2, 0.01987, 0.75428, 0.00528, 0.13497, 0.98963, 0.84887, 0.73157, 0.68004, 25, 49, "T", "B", "sc-like readlen", "illumina", "nextseq", "unknown", "cdna_unspecified", "nextera", "sc", "single_cell_droplet", "dropseq", null, "United States", "2022-11-12", "Adult", "Adult", "Liver", "Liver and Biliary System"], [72159, "SRR22266730", "SRX18242997", "SRS15738722", "SRP407581", "PRJNA900999", "Biliary epithelial cells are facultative liver stem cells during liver regeneration in adult zebrafish", "GSE217839", "Transcriptome Analysis", "Single cell sequencing was performed on the adult zebrafish liver  on both uninjured animals  and animals regenerating from hepatocyte ablation. Overall design: Samples were sequenced at 0  1  2  3  and 7 days post ablation. Mock treated and uninjured animals were also sequenced.", null, "pubmed:36625346", null, "uninjured  replicate 6  scRNAseq", "GSM6727442", null, "source name:liver|tissue:liver|cell type:all cells|genotype:TU|treatment:Uninjured", "uninjured  replicate 6  scRNAseq", "Fastq files were processed as described using the 2.4.0 version of the Drop seq tools  mapping the reads to GRCz11 with the default parameters. For the DigitalExpression command we set NUM CORE BARCODES=5000. We used STAR version 2.5.3 and fastqc version 0.11.2. Assembly: GRCz11 Supplementary files format and content: *.txt.gz Supplementary files format and content: Digital Gene Expression Matrix", "liver", null, "Adult zebrafish livers were enyzmatically dissociated and live  nucleated cells were enriched using FACS. The resultant single cell suspension was subjected to the Drop seq protocol. Beads were subjected to reverse transcription with Maxima H Minus Reverse Transcripase. cDNA was amplified with KAPA HiFi HotStart ReadyMix. Amplified cDNA was made into libraries using the Nextera XT DNA Library Preparation Kit  with the goal of capturing 1000 cells per library. Libraries were sequenced on an Illumina NextSeq 500 using the Single End 75bp kit with the following parameters: Read 1: 20 bp  Read 2: 50 bp  Read 1 Index: 8 bp  Custom Read 1 primer  GCCTGTCCGCGGAAGCAGTGGTATCAACGCAGAGTAC.", null, "tissue:liver|cell type:all cells|genotype:TU|treatment:Uninjured", "GSM6727442", "GSM6727442: uninjured  replicate 6  scRNAseq; Danio rerio; RNA Seq", "GSM6727442 r1", "GSM6727442", "1", "Adult zebrafish livers were enyzmatically dissociated and live  nucleated cells were enriched using FACS. The resultant single cell suspension was subjected to the Drop seq protocol. Beads were subjected to reverse transcription with Maxima H Minus Reverse Transcripase. cDNA was amplified with KAPA HiFi HotStart ReadyMix. Amplified cDNA was made into libraries using the Nextera XT DNA Library Preparation Kit  with the goal of capturing 1000 cells per library. Libraries were sequenced on an Illumina NextSeq 500 using the Single End 75bp kit with the following parameters: Read 1: 20 bp  Read 2: 50 bp  Read 1 Index: 8 bp  Custom Read 1 primer  GCCTGTCCGCGGAAGCAGTGGTATCAACGCAGAGTAC.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 500", null, "SRP407581", null, null, "20180316_LIB3D_IO5377-2_S3_R1_001.fastq.gz 20180316_LIB3D_IO5377-2_S3_R2_001.fastq.gz", "fastq fastq", 8019262192.0, 108368408.0, "GSM6727442 r1", "0:25 1:49", "A:2321354742;C:1785680783;G:1617017094;T:2294899508;N:310065", 25, 49, null, null, 2321354742, 1785680783, 1617017094, 2294899508, 310065, "SRX18242997", "SRS15738722", null, null, "Division of Genetics, Brigham and Women's Hospital", 2, 0.01825, 0.43176, 0.00603, 0.0356, 0.99054, 0.93872, 0.39005, 0.39912, 25, 49, "T", "B", "sc-like readlen", "illumina", "nextseq", "unknown", "cdna_unspecified", "nextera", "sc", "single_cell_droplet", "dropseq", null, "United States", "2022-11-12", "Adult", "Adult", "Liver", "Liver and Biliary System"], [72160, "SRR22266731", "SRX18242996", "SRS15738721", "SRP407581", "PRJNA900999", "Biliary epithelial cells are facultative liver stem cells during liver regeneration in adult zebrafish", "GSE217839", "Transcriptome Analysis", "Single cell sequencing was performed on the adult zebrafish liver  on both uninjured animals  and animals regenerating from hepatocyte ablation. Overall design: Samples were sequenced at 0  1  2  3  and 7 days post ablation. Mock treated and uninjured animals were also sequenced.", null, "pubmed:36625346", null, "uninjured  replicate 5  scRNAseq", "GSM6727441", null, "source name:liver|tissue:liver|cell type:all cells|genotype:TU|treatment:Uninjured", "uninjured  replicate 5  scRNAseq", "Fastq files were processed as described using the 2.4.0 version of the Drop seq tools  mapping the reads to GRCz11 with the default parameters. For the DigitalExpression command we set NUM CORE BARCODES=5000. We used STAR version 2.5.3 and fastqc version 0.11.2. Assembly: GRCz11 Supplementary files format and content: *.txt.gz Supplementary files format and content: Digital Gene Expression Matrix", "liver", null, "Adult zebrafish livers were enyzmatically dissociated and live  nucleated cells were enriched using FACS. The resultant single cell suspension was subjected to the Drop seq protocol. Beads were subjected to reverse transcription with Maxima H Minus Reverse Transcripase. cDNA was amplified with KAPA HiFi HotStart ReadyMix. Amplified cDNA was made into libraries using the Nextera XT DNA Library Preparation Kit  with the goal of capturing 1000 cells per library. Libraries were sequenced on an Illumina NextSeq 500 using the Single End 75bp kit with the following parameters: Read 1: 20 bp  Read 2: 50 bp  Read 1 Index: 8 bp  Custom Read 1 primer  GCCTGTCCGCGGAAGCAGTGGTATCAACGCAGAGTAC.", null, "tissue:liver|cell type:all cells|genotype:TU|treatment:Uninjured", "GSM6727441", "GSM6727441: uninjured  replicate 5  scRNAseq; Danio rerio; RNA Seq", "GSM6727441 r1", "GSM6727441", "1", "Adult zebrafish livers were enyzmatically dissociated and live  nucleated cells were enriched using FACS. The resultant single cell suspension was subjected to the Drop seq protocol. Beads were subjected to reverse transcription with Maxima H Minus Reverse Transcripase. cDNA was amplified with KAPA HiFi HotStart ReadyMix. Amplified cDNA was made into libraries using the Nextera XT DNA Library Preparation Kit  with the goal of capturing 1000 cells per library. Libraries were sequenced on an Illumina NextSeq 500 using the Single End 75bp kit with the following parameters: Read 1: 20 bp  Read 2: 50 bp  Read 1 Index: 8 bp  Custom Read 1 primer  GCCTGTCCGCGGAAGCAGTGGTATCAACGCAGAGTAC.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 500", null, "SRP407581", null, null, "20180316_LIB3C_IO5377-2_S2_R1_001.fastq.gz 20180316_LIB3C_IO5377-2_S2_R2_001.fastq.gz", "fastq fastq", 9922950894.0, 134093931.0, "GSM6727441 r1", "0:25 1:49", "A:2400612356;C:2523653639;G:2164027118;T:2834273253;N:384528", 25, 49, null, null, 2400612356, 2523653639, 2164027118, 2834273253, 384528, "SRX18242996", "SRS15738721", null, null, "Division of Genetics, Brigham and Women's Hospital", 2, 0.01765, 0.24689, 0.00828, 0.03521, 0.9891, 0.93519, 0.74426, 0.65974, 25, 49, "T", "B", "sc-like readlen", "illumina", "nextseq", "unknown", "cdna_unspecified", "nextera", "sc", "single_cell_droplet", "dropseq", null, "United States", "2022-11-12", "Adult", "Adult", "Liver", "Liver and Biliary System"], [72161, "SRR22266732", "SRX18242995", "SRS15738720", "SRP407581", "PRJNA900999", "Biliary epithelial cells are facultative liver stem cells during liver regeneration in adult zebrafish", "GSE217839", "Transcriptome Analysis", "Single cell sequencing was performed on the adult zebrafish liver  on both uninjured animals  and animals regenerating from hepatocyte ablation. Overall design: Samples were sequenced at 0  1  2  3  and 7 days post ablation. Mock treated and uninjured animals were also sequenced.", null, "pubmed:36625346", null, "uninjured  replicate 4  scRNAseq", "GSM6727440", null, "source name:liver|tissue:liver|cell type:all cells|genotype:TU|treatment:Uninjured", "uninjured  replicate 4  scRNAseq", "Fastq files were processed as described using the 2.4.0 version of the Drop seq tools  mapping the reads to GRCz11 with the default parameters. For the DigitalExpression command we set NUM CORE BARCODES=5000. We used STAR version 2.5.3 and fastqc version 0.11.2. Assembly: GRCz11 Supplementary files format and content: *.txt.gz Supplementary files format and content: Digital Gene Expression Matrix", "liver", null, "Adult zebrafish livers were enyzmatically dissociated and live  nucleated cells were enriched using FACS. The resultant single cell suspension was subjected to the Drop seq protocol. Beads were subjected to reverse transcription with Maxima H Minus Reverse Transcripase. cDNA was amplified with KAPA HiFi HotStart ReadyMix. Amplified cDNA was made into libraries using the Nextera XT DNA Library Preparation Kit  with the goal of capturing 1000 cells per library. Libraries were sequenced on an Illumina NextSeq 500 using the Single End 75bp kit with the following parameters: Read 1: 20 bp  Read 2: 50 bp  Read 1 Index: 8 bp  Custom Read 1 primer  GCCTGTCCGCGGAAGCAGTGGTATCAACGCAGAGTAC.", null, "tissue:liver|cell type:all cells|genotype:TU|treatment:Uninjured", "GSM6727440", "GSM6727440: uninjured  replicate 4  scRNAseq; Danio rerio; RNA Seq", "GSM6727440 r1", "GSM6727440", "1", "Adult zebrafish livers were enyzmatically dissociated and live  nucleated cells were enriched using FACS. The resultant single cell suspension was subjected to the Drop seq protocol. Beads were subjected to reverse transcription with Maxima H Minus Reverse Transcripase. cDNA was amplified with KAPA HiFi HotStart ReadyMix. Amplified cDNA was made into libraries using the Nextera XT DNA Library Preparation Kit  with the goal of capturing 1000 cells per library. Libraries were sequenced on an Illumina NextSeq 500 using the Single End 75bp kit with the following parameters: Read 1: 20 bp  Read 2: 50 bp  Read 1 Index: 8 bp  Custom Read 1 primer  GCCTGTCCGCGGAAGCAGTGGTATCAACGCAGAGTAC.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 500", null, "SRP407581", null, null, "20180316_LIB3A_IO5377-2_S1_R1_001.fastq.gz 20180316_LIB3A_IO5377-2_S1_R2_001.fastq.gz", "fastq fastq", 7562034174.0, 102189651.0, "GSM6727440 r1", "0:25 1:49", "A:2082168365;C:1773377474;G:1641155438;T:2065037229;N:295668", 25, 49, null, null, 2082168365, 1773377474, 1641155438, 2065037229, 295668, "SRX18242995", "SRS15738720", null, null, "Division of Genetics, Brigham and Women's Hospital", 2, 0.01855, 0.34293, 0.00452, 0.03498, 0.9876, 0.94, 0.33164, 0.69274, 25, 49, "T", "B", "sc-like readlen", "illumina", "nextseq", "unknown", "cdna_unspecified", "nextera", "sc", "single_cell_droplet", "dropseq", null, "United States", "2022-11-12", "Adult", "Adult", "Liver", "Liver and Biliary System"], [72162, "SRR22266733", "SRX18242994", "SRS15738719", "SRP407581", "PRJNA900999", "Biliary epithelial cells are facultative liver stem cells during liver regeneration in adult zebrafish", "GSE217839", "Transcriptome Analysis", "Single cell sequencing was performed on the adult zebrafish liver  on both uninjured animals  and animals regenerating from hepatocyte ablation. Overall design: Samples were sequenced at 0  1  2  3  and 7 days post ablation. Mock treated and uninjured animals were also sequenced.", null, "pubmed:36625346", null, "uninjured  replicate 3  scRNAseq", "GSM6727439", null, "source name:liver|tissue:liver|cell type:all cells|genotype:TU|treatment:Uninjured", "uninjured  replicate 3  scRNAseq", "Fastq files were processed as described using the 2.4.0 version of the Drop seq tools  mapping the reads to GRCz11 with the default parameters. For the DigitalExpression command we set NUM CORE BARCODES=5000. We used STAR version 2.5.3 and fastqc version 0.11.2. Assembly: GRCz11 Supplementary files format and content: *.txt.gz Supplementary files format and content: Digital Gene Expression Matrix", "liver", null, "Adult zebrafish livers were enyzmatically dissociated and live  nucleated cells were enriched using FACS. The resultant single cell suspension was subjected to the Drop seq protocol. Beads were subjected to reverse transcription with Maxima H Minus Reverse Transcripase. cDNA was amplified with KAPA HiFi HotStart ReadyMix. Amplified cDNA was made into libraries using the Nextera XT DNA Library Preparation Kit  with the goal of capturing 1000 cells per library. Libraries were sequenced on an Illumina NextSeq 500 using the Single End 75bp kit with the following parameters: Read 1: 20 bp  Read 2: 50 bp  Read 1 Index: 8 bp  Custom Read 1 primer  GCCTGTCCGCGGAAGCAGTGGTATCAACGCAGAGTAC.", null, "tissue:liver|cell type:all cells|genotype:TU|treatment:Uninjured", "GSM6727439", "GSM6727439: uninjured  replicate 3  scRNAseq; Danio rerio; RNA Seq", "GSM6727439 r1", "GSM6727439", "1", "Adult zebrafish livers were enyzmatically dissociated and live  nucleated cells were enriched using FACS. The resultant single cell suspension was subjected to the Drop seq protocol. Beads were subjected to reverse transcription with Maxima H Minus Reverse Transcripase. cDNA was amplified with KAPA HiFi HotStart ReadyMix. Amplified cDNA was made into libraries using the Nextera XT DNA Library Preparation Kit  with the goal of capturing 1000 cells per library. Libraries were sequenced on an Illumina NextSeq 500 using the Single End 75bp kit with the following parameters: Read 1: 20 bp  Read 2: 50 bp  Read 1 Index: 8 bp  Custom Read 1 primer  GCCTGTCCGCGGAAGCAGTGGTATCAACGCAGAGTAC.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 500", null, "SRP407581", null, null, "20180110_LIB2C_IO5170_S3_R1_001.fastq.gz 20180110_LIB2C_IO5170_S3_R2_001.fastq.gz", "fastq fastq", 6157056188.0, 83203462.0, "GSM6727439 r1", "0:25 1:49", "A:1886755825;C:1290134096;G:1216099330;T:1757471956;N:6594981", 25, 49, null, null, 1886755825, 1290134096, 1216099330, 1757471956, 6594981, "SRX18242994", "SRS15738719", null, null, "Division of Genetics, Brigham and Women's Hospital", 2, 0.0143, 0.53292, 0.0083, 0.06283, 0.99492, 0.94065, 0.83431, 0.72216, 25, 49, "T", "B", "sc-like readlen", "illumina", "nextseq", "unknown", "cdna_unspecified", "nextera", "sc", "single_cell_droplet", "dropseq", null, "United States", "2022-11-12", "Adult", "Adult", "Liver", "Liver and Biliary System"], [72163, "SRR22266734", "SRX18242993", "SRS15738718", "SRP407581", "PRJNA900999", "Biliary epithelial cells are facultative liver stem cells during liver regeneration in adult zebrafish", "GSE217839", "Transcriptome Analysis", "Single cell sequencing was performed on the adult zebrafish liver  on both uninjured animals  and animals regenerating from hepatocyte ablation. Overall design: Samples were sequenced at 0  1  2  3  and 7 days post ablation. Mock treated and uninjured animals were also sequenced.", null, "pubmed:36625346", null, "uninjured  replicate 2  scRNAseq", "GSM6727438", null, "source name:liver|tissue:liver|cell type:all cells|genotype:TU|treatment:Uninjured", "uninjured  replicate 2  scRNAseq", "Fastq files were processed as described using the 2.4.0 version of the Drop seq tools  mapping the reads to GRCz11 with the default parameters. For the DigitalExpression command we set NUM CORE BARCODES=5000. We used STAR version 2.5.3 and fastqc version 0.11.2. Assembly: GRCz11 Supplementary files format and content: *.txt.gz Supplementary files format and content: Digital Gene Expression Matrix", "liver", null, "Adult zebrafish livers were enyzmatically dissociated and live  nucleated cells were enriched using FACS. The resultant single cell suspension was subjected to the Drop seq protocol. Beads were subjected to reverse transcription with Maxima H Minus Reverse Transcripase. cDNA was amplified with KAPA HiFi HotStart ReadyMix. Amplified cDNA was made into libraries using the Nextera XT DNA Library Preparation Kit  with the goal of capturing 1000 cells per library. Libraries were sequenced on an Illumina NextSeq 500 using the Single End 75bp kit with the following parameters: Read 1: 20 bp  Read 2: 50 bp  Read 1 Index: 8 bp  Custom Read 1 primer  GCCTGTCCGCGGAAGCAGTGGTATCAACGCAGAGTAC.", null, "tissue:liver|cell type:all cells|genotype:TU|treatment:Uninjured", "GSM6727438", "GSM6727438: uninjured  replicate 2  scRNAseq; Danio rerio; RNA Seq", "GSM6727438 r1", "GSM6727438", "1", "Adult zebrafish livers were enyzmatically dissociated and live  nucleated cells were enriched using FACS. The resultant single cell suspension was subjected to the Drop seq protocol. Beads were subjected to reverse transcription with Maxima H Minus Reverse Transcripase. cDNA was amplified with KAPA HiFi HotStart ReadyMix. Amplified cDNA was made into libraries using the Nextera XT DNA Library Preparation Kit  with the goal of capturing 1000 cells per library. Libraries were sequenced on an Illumina NextSeq 500 using the Single End 75bp kit with the following parameters: Read 1: 20 bp  Read 2: 50 bp  Read 1 Index: 8 bp  Custom Read 1 primer  GCCTGTCCGCGGAAGCAGTGGTATCAACGCAGAGTAC.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 500", null, "SRP407581", null, null, "20180110_LIB2B_IO5170_S2_R1_001.fastq.gz 20180110_LIB2B_IO5170_S2_R2_001.fastq.gz", "fastq fastq", 4085425374.0, 55208451.0, "GSM6727438 r1", "0:25 1:49", "A:1148951414;C:943475846;G:839192575;T:1149676508;N:4129031", 25, 49, null, null, 1148951414, 943475846, 839192575, 1149676508, 4129031, "SRX18242993", "SRS15738718", null, null, "Division of Genetics, Brigham and Women's Hospital", 2, 0.01678, 0.38499, 0.00747, 0.03999, 0.99299, 0.95298, 0.82687, 0.74374, 25, 49, "T", "B", "sc-like readlen", "illumina", "nextseq", "unknown", "cdna_unspecified", "nextera", "sc", "single_cell_droplet", "dropseq", null, "United States", "2022-11-12", "Adult", "Adult", "Liver", "Liver and Biliary System"], [72164, "SRR22266735", "SRX18242992", "SRS15738717", "SRP407581", "PRJNA900999", "Biliary epithelial cells are facultative liver stem cells during liver regeneration in adult zebrafish", "GSE217839", "Transcriptome Analysis", "Single cell sequencing was performed on the adult zebrafish liver  on both uninjured animals  and animals regenerating from hepatocyte ablation. Overall design: Samples were sequenced at 0  1  2  3  and 7 days post ablation. Mock treated and uninjured animals were also sequenced.", null, "pubmed:36625346", null, "uninjured  replicate 1  scRNAseq", "GSM6727437", null, "source name:liver|tissue:liver|cell type:all cells|genotype:TU|treatment:Uninjured", "uninjured  replicate 1  scRNAseq", "Fastq files were processed as described using the 2.4.0 version of the Drop seq tools  mapping the reads to GRCz11 with the default parameters. For the DigitalExpression command we set NUM CORE BARCODES=5000. We used STAR version 2.5.3 and fastqc version 0.11.2. Assembly: GRCz11 Supplementary files format and content: *.txt.gz Supplementary files format and content: Digital Gene Expression Matrix", "liver", null, "Adult zebrafish livers were enyzmatically dissociated and live  nucleated cells were enriched using FACS. The resultant single cell suspension was subjected to the Drop seq protocol. Beads were subjected to reverse transcription with Maxima H Minus Reverse Transcripase. cDNA was amplified with KAPA HiFi HotStart ReadyMix. Amplified cDNA was made into libraries using the Nextera XT DNA Library Preparation Kit  with the goal of capturing 1000 cells per library. Libraries were sequenced on an Illumina NextSeq 500 using the Single End 75bp kit with the following parameters: Read 1: 20 bp  Read 2: 50 bp  Read 1 Index: 8 bp  Custom Read 1 primer  GCCTGTCCGCGGAAGCAGTGGTATCAACGCAGAGTAC.", null, "tissue:liver|cell type:all cells|genotype:TU|treatment:Uninjured", "GSM6727437", "GSM6727437: uninjured  replicate 1  scRNAseq; Danio rerio; RNA Seq", "GSM6727437 r1", "GSM6727437", "1", "Adult zebrafish livers were enyzmatically dissociated and live  nucleated cells were enriched using FACS. The resultant single cell suspension was subjected to the Drop seq protocol. Beads were subjected to reverse transcription with Maxima H Minus Reverse Transcripase. cDNA was amplified with KAPA HiFi HotStart ReadyMix. Amplified cDNA was made into libraries using the Nextera XT DNA Library Preparation Kit  with the goal of capturing 1000 cells per library. Libraries were sequenced on an Illumina NextSeq 500 using the Single End 75bp kit with the following parameters: Read 1: 20 bp  Read 2: 50 bp  Read 1 Index: 8 bp  Custom Read 1 primer  GCCTGTCCGCGGAAGCAGTGGTATCAACGCAGAGTAC.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 500", null, "SRP407581", null, null, "20180110_LIB2A_IO5170_S1_R1_001.fastq.gz 20180110_LIB2A_IO5170_S1_R2_001.fastq.gz", "fastq fastq", 5454978746.0, 73715929.0, "GSM6727437 r1", "0:25 1:49", "A:1662394748;C:1166773893;G:1080027941;T:1540187839;N:5594325", 25, 49, null, null, 1662394748, 1166773893, 1080027941, 1540187839, 5594325, "SRX18242992", "SRS15738717", null, null, "Division of Genetics, Brigham and Women's Hospital", 2, 0.02089, 0.45353, 0.01367, 0.0687, 0.99168, 0.93139, 0.76523, 0.68002, 25, 49, "T", "B", "sc-like readlen", "illumina", "nextseq", "unknown", "cdna_unspecified", "nextera", "sc", "single_cell_droplet", "dropseq", null, "United States", "2022-11-12", "Adult", "Adult", "Liver", "Liver and Biliary System"], [74480, "SRR23824324", "SRX19646191", "SRS17014001", "SRP426909", "PRJNA943553", "Single cell transcriptomic analyses of the dynamic local and systemic response to cardiac injury in mice and zebrafish [zebrafish]", "GSE227190", "Transcriptome Analysis", "Mice respond to myocardial infarction MI by generating a fibrotic scar in the heart. This is accompanied by systemic inflammation post the MI resolves. In contrast  zebrafish can fully regenerate their heart post heart injury. To further investigate the divergent species' responses to cardiac injury  single cell transcriptomic analyses were performed on the heart  blood  liver  kidney  and pancreatic islets. Overall design: Upon heart cryoinjury  cells from multiple organs heart  liver  pancreas  and whole kidney marrow were isolated for scRNA seq at 1 and 7 xxx post injury together with the uninjured controls.", "parent bioproject:PRJNA943547", "pubmed:39627536", null, "liver Ctrl replicate 2", "GSM7092955", null, "source name:Liver|tissue:Liver|cell type:whole organ|genotype:wild type|treatment:Heart cryoinjury", "liver Ctrl replicate 2", "Demultiplexing  alignment  filtering  and UMI counting was performed with Cell Ranger v6.0.0 using the cellranger count command. Sample integration batch correction  expression value normalization  clustering  cell type annotation and so on were done scater and scran. For more details and the cell type annotation information  see https://github.com/abcwcm/CZI MI mouseZebrafish Assembly: GRCz11 Supplementary files format and content: CellRanger produced filtered feature barcode matrix in H5 format.", "Liver", "Heart cryoinjury or untreated", "Heart ventricles were placed in the ice cold PBS buffer with 1% BSA. post several washes removing blood cells  ventricles were gently cut into small pieces. Tissues were incubated in digestion buffer 0.5 ml HBSS plus 0.26 U/ml Liberase DH [Roche] and 1% sheep serum for 45 minutes at 37\u00b0C while agitating at 750 rpm on an Eppendorf ThermoMixer. Supernatants were collected every 15 min and neutralized with 10% sheep serum  and cells were completely disaggregated by gently pipetting up and down. The dissociated cells were centrifuged at 200 g for 5 min at 4\u00b0C and re suspended in HBSS with 0.05% BSA. The cell suspension 2 ml was then gently added on top of 2 ml HBSS buffer with 7.5% BSA. post spinning down at 200 g for 5 min at 4\u00b0C  the pellet was resuspended in 500 \uf06dl and filtered through a 35 mm strainer.  Whole kidney marrow cells were isolated as described previously. Briefly  kidneys were collected on ice and homogenized immediately in PBS plus 0.05% BSA by using a 10ml syringe with 18 1/2 G. The dissociated cells were washed twice in PBS plus 0.05% BSA and spun down at 300 g for 5 min at 4\u00b0C. Pellets were re suspended in PBS plus 0.05% BSA and filtered through a 35 mm strainer. Livers were collected on ice and incubated in digestion buffer 0.5 ml HBSS plus 0.13 U/ml Liberase DH [Roche] and 1% sheep serum at room temperature. The cell lyses was gently stirred with a Spinbar magnetic stirring bar Bel Art Products  and the supernatant were collected every 5 minutes. The cell lysate was filtered through a 70 mm strainer and centrifuged at 150 g for 3 min at 4\u00b0C. Pellet was resuspended in PBS plus 0.05% BSA and then gently added on top of HBSS buffer with 7.5% BSA. post spinning down at 300 g for 5 min  pellets were washed one more time with PBS plus 0.05% BSA. post centrifuging at 300 g for 5 min  the resuspended cell lyses was filtered through a 35 mm strainer. Pancreatic cells were isolated using the same protocol as liver cells except for the initial spinning down at 300 g for 5 min. All suspended cells were stained with trypan blue and counted using a hemocytometer. Standard library construction protocol using 10x Genomics Chromium Single Cell 3\u2019 GEM  Library & Gel Bead Kit v3  and Chromium Single Cell B Chip Kit.", "Standard zebrafish husbandry protocol.", "tissue:Liver|cell type:whole organ|genotype:wild type|treatment:Heart cryoinjury", "GSM7092955", "GSM7092955: liver Ctrl replicate 2; Danio rerio; RNA Seq", "GSM7092955 r1", "GSM7092955", "1", "Heart ventricles were placed in the ice cold PBS buffer with 1% BSA. post several washes removing blood cells  ventricles were gently cut into small pieces. Tissues were incubated in digestion buffer 0.5 ml HBSS plus 0.26 U/ml Liberase DH [Roche] and 1% sheep serum for 45 minutes at 37\u00b0C while agitating at 750 rpm on an Eppendorf ThermoMixer. Supernatants were collected every 15 min and neutralized with 10% sheep serum  and cells were completely disaggregated by gently pipetting up and down. The dissociated cells were centrifuged at 200 g for 5 min at 4\u00b0C and re suspended in HBSS with 0.05% BSA. The cell suspension 2 ml was then gently added on top of 2 ml HBSS buffer with 7.5% BSA. post spinning down at 200 g for 5 min at 4\u00b0C  the pellet was resuspended in 500 \uf06dl and filtered through a 35 mm strainer.  Whole kidney marrow cells were isolated as described previously. Briefly  kidneys were collected on ice and homogenized immediately in PBS plus 0.05% BSA by using a 10ml syringe with 18 1/2 G. The dissociated cells were washed twice in PBS plus 0.05% BSA and spun down at 300 g for 5 min at 4\u00b0C. Pellets were re suspended in PBS plus 0.05% BSA and filtered through a 35 mm strainer. Livers were collected on ice and incubated in digestion buffer 0.5 ml HBSS plus 0.13 U/ml Liberase DH [Roche] and 1% sheep serum at room temperature. The cell lyses was gently stirred with a Spinbar magnetic stirring bar Bel Art Products  and the supernatant were collected every 5 minutes. The cell lysate was filtered through a 70 mm strainer and centrifuged at 150 g for 3 min at 4\u00b0C. Pellet was resuspended in PBS plus 0.05% BSA and then gently added on top of HBSS buffer with 7.5% BSA. post spinning down at 300 g for 5 min  pellets were washed one more time with PBS plus 0.05% BSA. post centrifuging at 300 g for 5 min  the resuspended cell lyses was filtered through a 35 mm strainer. Pancreatic cells were isolated using the same protocol as liver cells except for the initial spinning down at 300 g for 5 min. All suspended cells were stained with trypan blue and counted using a hemocytometer. Standard library construction protocol using 10x Genomics Chromium Single Cell three prime GEM  Library & Gel Bead Kit v3  and Chromium Single Cell B Chip Kit.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP426909", null, "loader:fastq load.py", "Ctrl-Liver-2_S7_L001_R1_001.fastq.gz Ctrl-Liver-2_S7_L001_R2_001.fastq.gz", "fastq fastq", 5104340986.0, 43257127.0, "GSM7092955 r1", "0:28 1:90", "A:1424416053;C:1192239684;G:1190425268;T:1297142390;N:117591", 28, 90, null, null, 1424416053, 1192239684, 1190425268, 1297142390, 117591, "SRX19646191", "SRS17014001", "SRA1603354", "Applied Bioinformatics Core, Applied Bioinformatics Core, Weill Cornell Medicine", "Applied Bioinformatics Core, Applied Bioinformatics Core, Weill Cornell Medicine", 2, 0.00469, 0.94025, 0.00107, 0.05406, 0.99533, 0.87308, 0.27077, 0.46309, 28, 90, "T", "B", "sc-like readlen", "illumina", "novaseq_era", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "United States", "2023-03-12", "Undetermined", "Undetermined", "Liver", "Liver and Biliary System"], [74481, "SRR23824325", "SRX19646191", "SRS17014001", "SRP426909", "PRJNA943553", "Single cell transcriptomic analyses of the dynamic local and systemic response to cardiac injury in mice and zebrafish [zebrafish]", "GSE227190", "Transcriptome Analysis", "Mice respond to myocardial infarction MI by generating a fibrotic scar in the heart. This is accompanied by systemic inflammation post the MI resolves. In contrast  zebrafish can fully regenerate their heart post heart injury. To further investigate the divergent species' responses to cardiac injury  single cell transcriptomic analyses were performed on the heart  blood  liver  kidney  and pancreatic islets. Overall design: Upon heart cryoinjury  cells from multiple organs heart  liver  pancreas  and whole kidney marrow were isolated for scRNA seq at 1 and 7 xxx post injury together with the uninjured controls.", "parent bioproject:PRJNA943547", "pubmed:39627536", null, "liver Ctrl replicate 2", "GSM7092955", null, "source name:Liver|tissue:Liver|cell type:whole organ|genotype:wild type|treatment:Heart cryoinjury", "liver Ctrl replicate 2", "Demultiplexing  alignment  filtering  and UMI counting was performed with Cell Ranger v6.0.0 using the cellranger count command. Sample integration batch correction  expression value normalization  clustering  cell type annotation and so on were done scater and scran. For more details and the cell type annotation information  see https://github.com/abcwcm/CZI MI mouseZebrafish Assembly: GRCz11 Supplementary files format and content: CellRanger produced filtered feature barcode matrix in H5 format.", "Liver", "Heart cryoinjury or untreated", "Heart ventricles were placed in the ice cold PBS buffer with 1% BSA. post several washes removing blood cells  ventricles were gently cut into small pieces. Tissues were incubated in digestion buffer 0.5 ml HBSS plus 0.26 U/ml Liberase DH [Roche] and 1% sheep serum for 45 minutes at 37\u00b0C while agitating at 750 rpm on an Eppendorf ThermoMixer. Supernatants were collected every 15 min and neutralized with 10% sheep serum  and cells were completely disaggregated by gently pipetting up and down. The dissociated cells were centrifuged at 200 g for 5 min at 4\u00b0C and re suspended in HBSS with 0.05% BSA. The cell suspension 2 ml was then gently added on top of 2 ml HBSS buffer with 7.5% BSA. post spinning down at 200 g for 5 min at 4\u00b0C  the pellet was resuspended in 500 \uf06dl and filtered through a 35 mm strainer.  Whole kidney marrow cells were isolated as described previously. Briefly  kidneys were collected on ice and homogenized immediately in PBS plus 0.05% BSA by using a 10ml syringe with 18 1/2 G. The dissociated cells were washed twice in PBS plus 0.05% BSA and spun down at 300 g for 5 min at 4\u00b0C. Pellets were re suspended in PBS plus 0.05% BSA and filtered through a 35 mm strainer. Livers were collected on ice and incubated in digestion buffer 0.5 ml HBSS plus 0.13 U/ml Liberase DH [Roche] and 1% sheep serum at room temperature. The cell lyses was gently stirred with a Spinbar magnetic stirring bar Bel Art Products  and the supernatant were collected every 5 minutes. The cell lysate was filtered through a 70 mm strainer and centrifuged at 150 g for 3 min at 4\u00b0C. Pellet was resuspended in PBS plus 0.05% BSA and then gently added on top of HBSS buffer with 7.5% BSA. post spinning down at 300 g for 5 min  pellets were washed one more time with PBS plus 0.05% BSA. post centrifuging at 300 g for 5 min  the resuspended cell lyses was filtered through a 35 mm strainer. Pancreatic cells were isolated using the same protocol as liver cells except for the initial spinning down at 300 g for 5 min. All suspended cells were stained with trypan blue and counted using a hemocytometer. Standard library construction protocol using 10x Genomics Chromium Single Cell 3\u2019 GEM  Library & Gel Bead Kit v3  and Chromium Single Cell B Chip Kit.", "Standard zebrafish husbandry protocol.", "tissue:Liver|cell type:whole organ|genotype:wild type|treatment:Heart cryoinjury", "GSM7092955", "GSM7092955: liver Ctrl replicate 2; Danio rerio; RNA Seq", "GSM7092955 r1", "GSM7092955", "1", "Heart ventricles were placed in the ice cold PBS buffer with 1% BSA. post several washes removing blood cells  ventricles were gently cut into small pieces. Tissues were incubated in digestion buffer 0.5 ml HBSS plus 0.26 U/ml Liberase DH [Roche] and 1% sheep serum for 45 minutes at 37\u00b0C while agitating at 750 rpm on an Eppendorf ThermoMixer. Supernatants were collected every 15 min and neutralized with 10% sheep serum  and cells were completely disaggregated by gently pipetting up and down. The dissociated cells were centrifuged at 200 g for 5 min at 4\u00b0C and re suspended in HBSS with 0.05% BSA. The cell suspension 2 ml was then gently added on top of 2 ml HBSS buffer with 7.5% BSA. post spinning down at 200 g for 5 min at 4\u00b0C  the pellet was resuspended in 500 \uf06dl and filtered through a 35 mm strainer.  Whole kidney marrow cells were isolated as described previously. Briefly  kidneys were collected on ice and homogenized immediately in PBS plus 0.05% BSA by using a 10ml syringe with 18 1/2 G. The dissociated cells were washed twice in PBS plus 0.05% BSA and spun down at 300 g for 5 min at 4\u00b0C. Pellets were re suspended in PBS plus 0.05% BSA and filtered through a 35 mm strainer. Livers were collected on ice and incubated in digestion buffer 0.5 ml HBSS plus 0.13 U/ml Liberase DH [Roche] and 1% sheep serum at room temperature. The cell lyses was gently stirred with a Spinbar magnetic stirring bar Bel Art Products  and the supernatant were collected every 5 minutes. The cell lysate was filtered through a 70 mm strainer and centrifuged at 150 g for 3 min at 4\u00b0C. Pellet was resuspended in PBS plus 0.05% BSA and then gently added on top of HBSS buffer with 7.5% BSA. post spinning down at 300 g for 5 min  pellets were washed one more time with PBS plus 0.05% BSA. post centrifuging at 300 g for 5 min  the resuspended cell lyses was filtered through a 35 mm strainer. Pancreatic cells were isolated using the same protocol as liver cells except for the initial spinning down at 300 g for 5 min. All suspended cells were stained with trypan blue and counted using a hemocytometer. Standard library construction protocol using 10x Genomics Chromium Single Cell three prime GEM  Library & Gel Bead Kit v3  and Chromium Single Cell B Chip Kit.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP426909", null, "loader:fastq load.py", "Ctrl-Liver-2_S7_L002_R1_001.fastq.gz Ctrl-Liver-2_S7_L002_R2_001.fastq.gz", "fastq fastq", 5141806812.0, 43574634.0, "GSM7092955 r2", "0:28 1:90", "A:1434538137;C:1200870495;G:1198733719;T:1307553313;N:111148", 28, 90, null, null, 1434538137, 1200870495, 1198733719, 1307553313, 111148, "SRX19646191", "SRS17014001", "SRA1603354", "Applied Bioinformatics Core, Applied Bioinformatics Core, Weill Cornell Medicine", "Applied Bioinformatics Core, Applied Bioinformatics Core, Weill Cornell Medicine", 2, 0.0048, 0.93996, 0.00104, 0.05254, 0.99515, 0.87499, 0.26301, 0.4732, 28, 90, "T", "B", "sc-like readlen", "illumina", "novaseq_era", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "United States", "2023-03-12", "Undetermined", "Undetermined", "Liver", "Liver and Biliary System"], [74482, "SRR23824326", "SRX19646191", "SRS17014001", "SRP426909", "PRJNA943553", "Single cell transcriptomic analyses of the dynamic local and systemic response to cardiac injury in mice and zebrafish [zebrafish]", "GSE227190", "Transcriptome Analysis", "Mice respond to myocardial infarction MI by generating a fibrotic scar in the heart. This is accompanied by systemic inflammation post the MI resolves. In contrast  zebrafish can fully regenerate their heart post heart injury. To further investigate the divergent species' responses to cardiac injury  single cell transcriptomic analyses were performed on the heart  blood  liver  kidney  and pancreatic islets. Overall design: Upon heart cryoinjury  cells from multiple organs heart  liver  pancreas  and whole kidney marrow were isolated for scRNA seq at 1 and 7 xxx post injury together with the uninjured controls.", "parent bioproject:PRJNA943547", "pubmed:39627536", null, "liver Ctrl replicate 2", "GSM7092955", null, "source name:Liver|tissue:Liver|cell type:whole organ|genotype:wild type|treatment:Heart cryoinjury", "liver Ctrl replicate 2", "Demultiplexing  alignment  filtering  and UMI counting was performed with Cell Ranger v6.0.0 using the cellranger count command. Sample integration batch correction  expression value normalization  clustering  cell type annotation and so on were done scater and scran. For more details and the cell type annotation information  see https://github.com/abcwcm/CZI MI mouseZebrafish Assembly: GRCz11 Supplementary files format and content: CellRanger produced filtered feature barcode matrix in H5 format.", "Liver", "Heart cryoinjury or untreated", "Heart ventricles were placed in the ice cold PBS buffer with 1% BSA. post several washes removing blood cells  ventricles were gently cut into small pieces. Tissues were incubated in digestion buffer 0.5 ml HBSS plus 0.26 U/ml Liberase DH [Roche] and 1% sheep serum for 45 minutes at 37\u00b0C while agitating at 750 rpm on an Eppendorf ThermoMixer. Supernatants were collected every 15 min and neutralized with 10% sheep serum  and cells were completely disaggregated by gently pipetting up and down. The dissociated cells were centrifuged at 200 g for 5 min at 4\u00b0C and re suspended in HBSS with 0.05% BSA. The cell suspension 2 ml was then gently added on top of 2 ml HBSS buffer with 7.5% BSA. post spinning down at 200 g for 5 min at 4\u00b0C  the pellet was resuspended in 500 \uf06dl and filtered through a 35 mm strainer.  Whole kidney marrow cells were isolated as described previously. Briefly  kidneys were collected on ice and homogenized immediately in PBS plus 0.05% BSA by using a 10ml syringe with 18 1/2 G. The dissociated cells were washed twice in PBS plus 0.05% BSA and spun down at 300 g for 5 min at 4\u00b0C. Pellets were re suspended in PBS plus 0.05% BSA and filtered through a 35 mm strainer. Livers were collected on ice and incubated in digestion buffer 0.5 ml HBSS plus 0.13 U/ml Liberase DH [Roche] and 1% sheep serum at room temperature. The cell lyses was gently stirred with a Spinbar magnetic stirring bar Bel Art Products  and the supernatant were collected every 5 minutes. The cell lysate was filtered through a 70 mm strainer and centrifuged at 150 g for 3 min at 4\u00b0C. Pellet was resuspended in PBS plus 0.05% BSA and then gently added on top of HBSS buffer with 7.5% BSA. post spinning down at 300 g for 5 min  pellets were washed one more time with PBS plus 0.05% BSA. post centrifuging at 300 g for 5 min  the resuspended cell lyses was filtered through a 35 mm strainer. Pancreatic cells were isolated using the same protocol as liver cells except for the initial spinning down at 300 g for 5 min. All suspended cells were stained with trypan blue and counted using a hemocytometer. Standard library construction protocol using 10x Genomics Chromium Single Cell 3\u2019 GEM  Library & Gel Bead Kit v3  and Chromium Single Cell B Chip Kit.", "Standard zebrafish husbandry protocol.", "tissue:Liver|cell type:whole organ|genotype:wild type|treatment:Heart cryoinjury", "GSM7092955", "GSM7092955: liver Ctrl replicate 2; Danio rerio; RNA Seq", "GSM7092955 r1", "GSM7092955", "1", "Heart ventricles were placed in the ice cold PBS buffer with 1% BSA. post several washes removing blood cells  ventricles were gently cut into small pieces. Tissues were incubated in digestion buffer 0.5 ml HBSS plus 0.26 U/ml Liberase DH [Roche] and 1% sheep serum for 45 minutes at 37\u00b0C while agitating at 750 rpm on an Eppendorf ThermoMixer. Supernatants were collected every 15 min and neutralized with 10% sheep serum  and cells were completely disaggregated by gently pipetting up and down. The dissociated cells were centrifuged at 200 g for 5 min at 4\u00b0C and re suspended in HBSS with 0.05% BSA. The cell suspension 2 ml was then gently added on top of 2 ml HBSS buffer with 7.5% BSA. post spinning down at 200 g for 5 min at 4\u00b0C  the pellet was resuspended in 500 \uf06dl and filtered through a 35 mm strainer.  Whole kidney marrow cells were isolated as described previously. Briefly  kidneys were collected on ice and homogenized immediately in PBS plus 0.05% BSA by using a 10ml syringe with 18 1/2 G. The dissociated cells were washed twice in PBS plus 0.05% BSA and spun down at 300 g for 5 min at 4\u00b0C. Pellets were re suspended in PBS plus 0.05% BSA and filtered through a 35 mm strainer. Livers were collected on ice and incubated in digestion buffer 0.5 ml HBSS plus 0.13 U/ml Liberase DH [Roche] and 1% sheep serum at room temperature. The cell lyses was gently stirred with a Spinbar magnetic stirring bar Bel Art Products  and the supernatant were collected every 5 minutes. The cell lysate was filtered through a 70 mm strainer and centrifuged at 150 g for 3 min at 4\u00b0C. Pellet was resuspended in PBS plus 0.05% BSA and then gently added on top of HBSS buffer with 7.5% BSA. post spinning down at 300 g for 5 min  pellets were washed one more time with PBS plus 0.05% BSA. post centrifuging at 300 g for 5 min  the resuspended cell lyses was filtered through a 35 mm strainer. Pancreatic cells were isolated using the same protocol as liver cells except for the initial spinning down at 300 g for 5 min. All suspended cells were stained with trypan blue and counted using a hemocytometer. Standard library construction protocol using 10x Genomics Chromium Single Cell three prime GEM  Library & Gel Bead Kit v3  and Chromium Single Cell B Chip Kit.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP426909", null, "loader:fastq load.py", "Ctrl-Liver-2_S7_L003_R1_001.fastq.gz Ctrl-Liver-2_S7_L003_R2_001.fastq.gz", "fastq fastq", 5192564158.0, 44004781.0, "GSM7092955 r3", "0:28 1:90", "A:1448034478;C:1213477598;G:1211749223;T:1319197183;N:105676", 28, 90, null, null, 1448034478, 1213477598, 1211749223, 1319197183, 105676, "SRX19646191", "SRS17014001", "SRA1603354", "Applied Bioinformatics Core, Applied Bioinformatics Core, Weill Cornell Medicine", "Applied Bioinformatics Core, Applied Bioinformatics Core, Weill Cornell Medicine", 2, 0.00504, 0.9401, 0.00113, 0.05317, 0.99462, 0.87361, 0.27858, 0.47856, 28, 90, "T", "B", "sc-like readlen", "illumina", "novaseq_era", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "United States", "2023-03-12", "Undetermined", "Undetermined", "Liver", "Liver and Biliary System"], [74483, "SRR23824327", "SRX19646191", "SRS17014001", "SRP426909", "PRJNA943553", "Single cell transcriptomic analyses of the dynamic local and systemic response to cardiac injury in mice and zebrafish [zebrafish]", "GSE227190", "Transcriptome Analysis", "Mice respond to myocardial infarction MI by generating a fibrotic scar in the heart. This is accompanied by systemic inflammation post the MI resolves. In contrast  zebrafish can fully regenerate their heart post heart injury. To further investigate the divergent species' responses to cardiac injury  single cell transcriptomic analyses were performed on the heart  blood  liver  kidney  and pancreatic islets. Overall design: Upon heart cryoinjury  cells from multiple organs heart  liver  pancreas  and whole kidney marrow were isolated for scRNA seq at 1 and 7 xxx post injury together with the uninjured controls.", "parent bioproject:PRJNA943547", "pubmed:39627536", null, "liver Ctrl replicate 2", "GSM7092955", null, "source name:Liver|tissue:Liver|cell type:whole organ|genotype:wild type|treatment:Heart cryoinjury", "liver Ctrl replicate 2", "Demultiplexing  alignment  filtering  and UMI counting was performed with Cell Ranger v6.0.0 using the cellranger count command. Sample integration batch correction  expression value normalization  clustering  cell type annotation and so on were done scater and scran. For more details and the cell type annotation information  see https://github.com/abcwcm/CZI MI mouseZebrafish Assembly: GRCz11 Supplementary files format and content: CellRanger produced filtered feature barcode matrix in H5 format.", "Liver", "Heart cryoinjury or untreated", "Heart ventricles were placed in the ice cold PBS buffer with 1% BSA. post several washes removing blood cells  ventricles were gently cut into small pieces. Tissues were incubated in digestion buffer 0.5 ml HBSS plus 0.26 U/ml Liberase DH [Roche] and 1% sheep serum for 45 minutes at 37\u00b0C while agitating at 750 rpm on an Eppendorf ThermoMixer. Supernatants were collected every 15 min and neutralized with 10% sheep serum  and cells were completely disaggregated by gently pipetting up and down. The dissociated cells were centrifuged at 200 g for 5 min at 4\u00b0C and re suspended in HBSS with 0.05% BSA. The cell suspension 2 ml was then gently added on top of 2 ml HBSS buffer with 7.5% BSA. post spinning down at 200 g for 5 min at 4\u00b0C  the pellet was resuspended in 500 \uf06dl and filtered through a 35 mm strainer.  Whole kidney marrow cells were isolated as described previously. Briefly  kidneys were collected on ice and homogenized immediately in PBS plus 0.05% BSA by using a 10ml syringe with 18 1/2 G. The dissociated cells were washed twice in PBS plus 0.05% BSA and spun down at 300 g for 5 min at 4\u00b0C. Pellets were re suspended in PBS plus 0.05% BSA and filtered through a 35 mm strainer. Livers were collected on ice and incubated in digestion buffer 0.5 ml HBSS plus 0.13 U/ml Liberase DH [Roche] and 1% sheep serum at room temperature. The cell lyses was gently stirred with a Spinbar magnetic stirring bar Bel Art Products  and the supernatant were collected every 5 minutes. The cell lysate was filtered through a 70 mm strainer and centrifuged at 150 g for 3 min at 4\u00b0C. Pellet was resuspended in PBS plus 0.05% BSA and then gently added on top of HBSS buffer with 7.5% BSA. post spinning down at 300 g for 5 min  pellets were washed one more time with PBS plus 0.05% BSA. post centrifuging at 300 g for 5 min  the resuspended cell lyses was filtered through a 35 mm strainer. Pancreatic cells were isolated using the same protocol as liver cells except for the initial spinning down at 300 g for 5 min. All suspended cells were stained with trypan blue and counted using a hemocytometer. Standard library construction protocol using 10x Genomics Chromium Single Cell 3\u2019 GEM  Library & Gel Bead Kit v3  and Chromium Single Cell B Chip Kit.", "Standard zebrafish husbandry protocol.", "tissue:Liver|cell type:whole organ|genotype:wild type|treatment:Heart cryoinjury", "GSM7092955", "GSM7092955: liver Ctrl replicate 2; Danio rerio; RNA Seq", "GSM7092955 r1", "GSM7092955", "1", "Heart ventricles were placed in the ice cold PBS buffer with 1% BSA. post several washes removing blood cells  ventricles were gently cut into small pieces. Tissues were incubated in digestion buffer 0.5 ml HBSS plus 0.26 U/ml Liberase DH [Roche] and 1% sheep serum for 45 minutes at 37\u00b0C while agitating at 750 rpm on an Eppendorf ThermoMixer. Supernatants were collected every 15 min and neutralized with 10% sheep serum  and cells were completely disaggregated by gently pipetting up and down. The dissociated cells were centrifuged at 200 g for 5 min at 4\u00b0C and re suspended in HBSS with 0.05% BSA. The cell suspension 2 ml was then gently added on top of 2 ml HBSS buffer with 7.5% BSA. post spinning down at 200 g for 5 min at 4\u00b0C  the pellet was resuspended in 500 \uf06dl and filtered through a 35 mm strainer.  Whole kidney marrow cells were isolated as described previously. Briefly  kidneys were collected on ice and homogenized immediately in PBS plus 0.05% BSA by using a 10ml syringe with 18 1/2 G. The dissociated cells were washed twice in PBS plus 0.05% BSA and spun down at 300 g for 5 min at 4\u00b0C. Pellets were re suspended in PBS plus 0.05% BSA and filtered through a 35 mm strainer. Livers were collected on ice and incubated in digestion buffer 0.5 ml HBSS plus 0.13 U/ml Liberase DH [Roche] and 1% sheep serum at room temperature. The cell lyses was gently stirred with a Spinbar magnetic stirring bar Bel Art Products  and the supernatant were collected every 5 minutes. The cell lysate was filtered through a 70 mm strainer and centrifuged at 150 g for 3 min at 4\u00b0C. Pellet was resuspended in PBS plus 0.05% BSA and then gently added on top of HBSS buffer with 7.5% BSA. post spinning down at 300 g for 5 min  pellets were washed one more time with PBS plus 0.05% BSA. post centrifuging at 300 g for 5 min  the resuspended cell lyses was filtered through a 35 mm strainer. Pancreatic cells were isolated using the same protocol as liver cells except for the initial spinning down at 300 g for 5 min. All suspended cells were stained with trypan blue and counted using a hemocytometer. Standard library construction protocol using 10x Genomics Chromium Single Cell three prime GEM  Library & Gel Bead Kit v3  and Chromium Single Cell B Chip Kit.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP426909", null, "loader:fastq load.py", "Ctrl-Liver-2_S7_L004_R1_001.fastq.gz Ctrl-Liver-2_S7_L004_R2_001.fastq.gz", "fastq fastq", 5107398366.0, 43283037.0, "GSM7092955 r4", "0:28 1:90", "A:1425848029;C:1192174260;G:1189956127;T:1299322516;N:97434", 28, 90, null, null, 1425848029, 1192174260, 1189956127, 1299322516, 97434, "SRX19646191", "SRS17014001", "SRA1603354", "Applied Bioinformatics Core, Applied Bioinformatics Core, Weill Cornell Medicine", "Applied Bioinformatics Core, Applied Bioinformatics Core, Weill Cornell Medicine", 2, 0.00498, 0.9401, 0.00127, 0.05293, 0.99494, 0.87572, 0.27094, 0.47517, 28, 90, "T", "B", "sc-like readlen", "illumina", "novaseq_era", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "United States", "2023-03-12", "Undetermined", "Undetermined", "Liver", "Liver and Biliary System"], [74484, "SRR23824328", "SRX19646190", "SRS17014000", "SRP426909", "PRJNA943553", "Single cell transcriptomic analyses of the dynamic local and systemic response to cardiac injury in mice and zebrafish [zebrafish]", "GSE227190", "Transcriptome Analysis", "Mice respond to myocardial infarction MI by generating a fibrotic scar in the heart. This is accompanied by systemic inflammation post the MI resolves. In contrast  zebrafish can fully regenerate their heart post heart injury. To further investigate the divergent species' responses to cardiac injury  single cell transcriptomic analyses were performed on the heart  blood  liver  kidney  and pancreatic islets. Overall design: Upon heart cryoinjury  cells from multiple organs heart  liver  pancreas  and whole kidney marrow were isolated for scRNA seq at 1 and 7 xxx post injury together with the uninjured controls.", "parent bioproject:PRJNA943547", "pubmed:39627536", null, "liver Ctrl replicate 1", "GSM7092954", null, "source name:Liver|tissue:Liver|cell type:whole organ|genotype:wild type|treatment:Heart cryoinjury", "liver Ctrl replicate 1", "Demultiplexing  alignment  filtering  and UMI counting was performed with Cell Ranger v6.0.0 using the cellranger count command. Sample integration batch correction  expression value normalization  clustering  cell type annotation and so on were done scater and scran. For more details and the cell type annotation information  see https://github.com/abcwcm/CZI MI mouseZebrafish Assembly: GRCz11 Supplementary files format and content: CellRanger produced filtered feature barcode matrix in H5 format.", "Liver", "Heart cryoinjury or untreated", "Heart ventricles were placed in the ice cold PBS buffer with 1% BSA. post several washes removing blood cells  ventricles were gently cut into small pieces. Tissues were incubated in digestion buffer 0.5 ml HBSS plus 0.26 U/ml Liberase DH [Roche] and 1% sheep serum for 45 minutes at 37\u00b0C while agitating at 750 rpm on an Eppendorf ThermoMixer. Supernatants were collected every 15 min and neutralized with 10% sheep serum  and cells were completely disaggregated by gently pipetting up and down. The dissociated cells were centrifuged at 200 g for 5 min at 4\u00b0C and re suspended in HBSS with 0.05% BSA. The cell suspension 2 ml was then gently added on top of 2 ml HBSS buffer with 7.5% BSA. post spinning down at 200 g for 5 min at 4\u00b0C  the pellet was resuspended in 500 \uf06dl and filtered through a 35 mm strainer.  Whole kidney marrow cells were isolated as described previously. Briefly  kidneys were collected on ice and homogenized immediately in PBS plus 0.05% BSA by using a 10ml syringe with 18 1/2 G. The dissociated cells were washed twice in PBS plus 0.05% BSA and spun down at 300 g for 5 min at 4\u00b0C. Pellets were re suspended in PBS plus 0.05% BSA and filtered through a 35 mm strainer. Livers were collected on ice and incubated in digestion buffer 0.5 ml HBSS plus 0.13 U/ml Liberase DH [Roche] and 1% sheep serum at room temperature. The cell lyses was gently stirred with a Spinbar magnetic stirring bar Bel Art Products  and the supernatant were collected every 5 minutes. The cell lysate was filtered through a 70 mm strainer and centrifuged at 150 g for 3 min at 4\u00b0C. Pellet was resuspended in PBS plus 0.05% BSA and then gently added on top of HBSS buffer with 7.5% BSA. post spinning down at 300 g for 5 min  pellets were washed one more time with PBS plus 0.05% BSA. post centrifuging at 300 g for 5 min  the resuspended cell lyses was filtered through a 35 mm strainer. Pancreatic cells were isolated using the same protocol as liver cells except for the initial spinning down at 300 g for 5 min. All suspended cells were stained with trypan blue and counted using a hemocytometer. Standard library construction protocol using 10x Genomics Chromium Single Cell 3\u2019 GEM  Library & Gel Bead Kit v3  and Chromium Single Cell B Chip Kit.", "Standard zebrafish husbandry protocol.", "tissue:Liver|cell type:whole organ|genotype:wild type|treatment:Heart cryoinjury", "GSM7092954", "GSM7092954: liver Ctrl replicate 1; Danio rerio; RNA Seq", "GSM7092954 r1", "GSM7092954", "1", "Heart ventricles were placed in the ice cold PBS buffer with 1% BSA. post several washes removing blood cells  ventricles were gently cut into small pieces. Tissues were incubated in digestion buffer 0.5 ml HBSS plus 0.26 U/ml Liberase DH [Roche] and 1% sheep serum for 45 minutes at 37\u00b0C while agitating at 750 rpm on an Eppendorf ThermoMixer. Supernatants were collected every 15 min and neutralized with 10% sheep serum  and cells were completely disaggregated by gently pipetting up and down. The dissociated cells were centrifuged at 200 g for 5 min at 4\u00b0C and re suspended in HBSS with 0.05% BSA. The cell suspension 2 ml was then gently added on top of 2 ml HBSS buffer with 7.5% BSA. post spinning down at 200 g for 5 min at 4\u00b0C  the pellet was resuspended in 500 \uf06dl and filtered through a 35 mm strainer.  Whole kidney marrow cells were isolated as described previously. Briefly  kidneys were collected on ice and homogenized immediately in PBS plus 0.05% BSA by using a 10ml syringe with 18 1/2 G. The dissociated cells were washed twice in PBS plus 0.05% BSA and spun down at 300 g for 5 min at 4\u00b0C. Pellets were re suspended in PBS plus 0.05% BSA and filtered through a 35 mm strainer. Livers were collected on ice and incubated in digestion buffer 0.5 ml HBSS plus 0.13 U/ml Liberase DH [Roche] and 1% sheep serum at room temperature. The cell lyses was gently stirred with a Spinbar magnetic stirring bar Bel Art Products  and the supernatant were collected every 5 minutes. The cell lysate was filtered through a 70 mm strainer and centrifuged at 150 g for 3 min at 4\u00b0C. Pellet was resuspended in PBS plus 0.05% BSA and then gently added on top of HBSS buffer with 7.5% BSA. post spinning down at 300 g for 5 min  pellets were washed one more time with PBS plus 0.05% BSA. post centrifuging at 300 g for 5 min  the resuspended cell lyses was filtered through a 35 mm strainer. Pancreatic cells were isolated using the same protocol as liver cells except for the initial spinning down at 300 g for 5 min. All suspended cells were stained with trypan blue and counted using a hemocytometer. Standard library construction protocol using 10x Genomics Chromium Single Cell three prime GEM  Library & Gel Bead Kit v3  and Chromium Single Cell B Chip Kit.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP426909", null, "loader:fastq load.py", "Ctrl-Liver-1_S3_L001_R1_001.fastq.gz Ctrl-Liver-1_S3_L001_R2_001.fastq.gz", "fastq fastq", 4496614672.0, 38106904.0, "GSM7092954 r1", "0:28 1:90", "A:1236795920;C:1061685679;G:1067304176;T:1130725493;N:103404", 28, 90, null, null, 1236795920, 1061685679, 1067304176, 1130725493, 103404, "SRX19646190", "SRS17014000", "SRA1603354", "Applied Bioinformatics Core, Applied Bioinformatics Core, Weill Cornell Medicine", "Applied Bioinformatics Core, Applied Bioinformatics Core, Weill Cornell Medicine", 2, 0.00769, 0.94857, 0.00194, 0.0642, 0.9948, 0.87919, 0.26391, 0.47988, 28, 90, "T", "B", "sc-like readlen", "illumina", "novaseq_era", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "United States", "2023-03-12", "Undetermined", "Undetermined", "Liver", "Liver and Biliary System"], [74485, "SRR23824329", "SRX19646190", "SRS17014000", "SRP426909", "PRJNA943553", "Single cell transcriptomic analyses of the dynamic local and systemic response to cardiac injury in mice and zebrafish [zebrafish]", "GSE227190", "Transcriptome Analysis", "Mice respond to myocardial infarction MI by generating a fibrotic scar in the heart. This is accompanied by systemic inflammation post the MI resolves. In contrast  zebrafish can fully regenerate their heart post heart injury. To further investigate the divergent species' responses to cardiac injury  single cell transcriptomic analyses were performed on the heart  blood  liver  kidney  and pancreatic islets. Overall design: Upon heart cryoinjury  cells from multiple organs heart  liver  pancreas  and whole kidney marrow were isolated for scRNA seq at 1 and 7 xxx post injury together with the uninjured controls.", "parent bioproject:PRJNA943547", "pubmed:39627536", null, "liver Ctrl replicate 1", "GSM7092954", null, "source name:Liver|tissue:Liver|cell type:whole organ|genotype:wild type|treatment:Heart cryoinjury", "liver Ctrl replicate 1", "Demultiplexing  alignment  filtering  and UMI counting was performed with Cell Ranger v6.0.0 using the cellranger count command. Sample integration batch correction  expression value normalization  clustering  cell type annotation and so on were done scater and scran. For more details and the cell type annotation information  see https://github.com/abcwcm/CZI MI mouseZebrafish Assembly: GRCz11 Supplementary files format and content: CellRanger produced filtered feature barcode matrix in H5 format.", "Liver", "Heart cryoinjury or untreated", "Heart ventricles were placed in the ice cold PBS buffer with 1% BSA. post several washes removing blood cells  ventricles were gently cut into small pieces. Tissues were incubated in digestion buffer 0.5 ml HBSS plus 0.26 U/ml Liberase DH [Roche] and 1% sheep serum for 45 minutes at 37\u00b0C while agitating at 750 rpm on an Eppendorf ThermoMixer. Supernatants were collected every 15 min and neutralized with 10% sheep serum  and cells were completely disaggregated by gently pipetting up and down. The dissociated cells were centrifuged at 200 g for 5 min at 4\u00b0C and re suspended in HBSS with 0.05% BSA. The cell suspension 2 ml was then gently added on top of 2 ml HBSS buffer with 7.5% BSA. post spinning down at 200 g for 5 min at 4\u00b0C  the pellet was resuspended in 500 \uf06dl and filtered through a 35 mm strainer.  Whole kidney marrow cells were isolated as described previously. Briefly  kidneys were collected on ice and homogenized immediately in PBS plus 0.05% BSA by using a 10ml syringe with 18 1/2 G. The dissociated cells were washed twice in PBS plus 0.05% BSA and spun down at 300 g for 5 min at 4\u00b0C. Pellets were re suspended in PBS plus 0.05% BSA and filtered through a 35 mm strainer. Livers were collected on ice and incubated in digestion buffer 0.5 ml HBSS plus 0.13 U/ml Liberase DH [Roche] and 1% sheep serum at room temperature. The cell lyses was gently stirred with a Spinbar magnetic stirring bar Bel Art Products  and the supernatant were collected every 5 minutes. The cell lysate was filtered through a 70 mm strainer and centrifuged at 150 g for 3 min at 4\u00b0C. Pellet was resuspended in PBS plus 0.05% BSA and then gently added on top of HBSS buffer with 7.5% BSA. post spinning down at 300 g for 5 min  pellets were washed one more time with PBS plus 0.05% BSA. post centrifuging at 300 g for 5 min  the resuspended cell lyses was filtered through a 35 mm strainer. Pancreatic cells were isolated using the same protocol as liver cells except for the initial spinning down at 300 g for 5 min. All suspended cells were stained with trypan blue and counted using a hemocytometer. Standard library construction protocol using 10x Genomics Chromium Single Cell 3\u2019 GEM  Library & Gel Bead Kit v3  and Chromium Single Cell B Chip Kit.", "Standard zebrafish husbandry protocol.", "tissue:Liver|cell type:whole organ|genotype:wild type|treatment:Heart cryoinjury", "GSM7092954", "GSM7092954: liver Ctrl replicate 1; Danio rerio; RNA Seq", "GSM7092954 r1", "GSM7092954", "1", "Heart ventricles were placed in the ice cold PBS buffer with 1% BSA. post several washes removing blood cells  ventricles were gently cut into small pieces. Tissues were incubated in digestion buffer 0.5 ml HBSS plus 0.26 U/ml Liberase DH [Roche] and 1% sheep serum for 45 minutes at 37\u00b0C while agitating at 750 rpm on an Eppendorf ThermoMixer. Supernatants were collected every 15 min and neutralized with 10% sheep serum  and cells were completely disaggregated by gently pipetting up and down. The dissociated cells were centrifuged at 200 g for 5 min at 4\u00b0C and re suspended in HBSS with 0.05% BSA. The cell suspension 2 ml was then gently added on top of 2 ml HBSS buffer with 7.5% BSA. post spinning down at 200 g for 5 min at 4\u00b0C  the pellet was resuspended in 500 \uf06dl and filtered through a 35 mm strainer.  Whole kidney marrow cells were isolated as described previously. Briefly  kidneys were collected on ice and homogenized immediately in PBS plus 0.05% BSA by using a 10ml syringe with 18 1/2 G. The dissociated cells were washed twice in PBS plus 0.05% BSA and spun down at 300 g for 5 min at 4\u00b0C. Pellets were re suspended in PBS plus 0.05% BSA and filtered through a 35 mm strainer. Livers were collected on ice and incubated in digestion buffer 0.5 ml HBSS plus 0.13 U/ml Liberase DH [Roche] and 1% sheep serum at room temperature. The cell lyses was gently stirred with a Spinbar magnetic stirring bar Bel Art Products  and the supernatant were collected every 5 minutes. The cell lysate was filtered through a 70 mm strainer and centrifuged at 150 g for 3 min at 4\u00b0C. Pellet was resuspended in PBS plus 0.05% BSA and then gently added on top of HBSS buffer with 7.5% BSA. post spinning down at 300 g for 5 min  pellets were washed one more time with PBS plus 0.05% BSA. post centrifuging at 300 g for 5 min  the resuspended cell lyses was filtered through a 35 mm strainer. Pancreatic cells were isolated using the same protocol as liver cells except for the initial spinning down at 300 g for 5 min. All suspended cells were stained with trypan blue and counted using a hemocytometer. Standard library construction protocol using 10x Genomics Chromium Single Cell three prime GEM  Library & Gel Bead Kit v3  and Chromium Single Cell B Chip Kit.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP426909", null, "loader:fastq load.py", "Ctrl-Liver-1_S3_L002_R2_001.fastq.gz Ctrl-Liver-1_S3_L002_R1_001.fastq.gz", "fastq fastq", 4500848984.0, 38142788.0, "GSM7092954 r2", "0:28 1:90", "A:1237725640;C:1062772315;G:1068127847;T:1132125691;N:97491", 28, 90, null, null, 1237725640, 1062772315, 1068127847, 1132125691, 97491, "SRX19646190", "SRS17014000", "SRA1603354", "Applied Bioinformatics Core, Applied Bioinformatics Core, Weill Cornell Medicine", "Applied Bioinformatics Core, Applied Bioinformatics Core, Weill Cornell Medicine", 2, 0.00735, 0.94796, 0.00174, 0.06344, 0.99504, 0.87706, 0.23275, 0.47453, 28, 90, "T", "B", "sc-like readlen", "illumina", "novaseq_era", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "United States", "2023-03-12", "Undetermined", "Undetermined", "Liver", "Liver and Biliary System"], [74486, "SRR23824330", "SRX19646190", "SRS17014000", "SRP426909", "PRJNA943553", "Single cell transcriptomic analyses of the dynamic local and systemic response to cardiac injury in mice and zebrafish [zebrafish]", "GSE227190", "Transcriptome Analysis", "Mice respond to myocardial infarction MI by generating a fibrotic scar in the heart. This is accompanied by systemic inflammation post the MI resolves. In contrast  zebrafish can fully regenerate their heart post heart injury. To further investigate the divergent species' responses to cardiac injury  single cell transcriptomic analyses were performed on the heart  blood  liver  kidney  and pancreatic islets. Overall design: Upon heart cryoinjury  cells from multiple organs heart  liver  pancreas  and whole kidney marrow were isolated for scRNA seq at 1 and 7 xxx post injury together with the uninjured controls.", "parent bioproject:PRJNA943547", "pubmed:39627536", null, "liver Ctrl replicate 1", "GSM7092954", null, "source name:Liver|tissue:Liver|cell type:whole organ|genotype:wild type|treatment:Heart cryoinjury", "liver Ctrl replicate 1", "Demultiplexing  alignment  filtering  and UMI counting was performed with Cell Ranger v6.0.0 using the cellranger count command. Sample integration batch correction  expression value normalization  clustering  cell type annotation and so on were done scater and scran. For more details and the cell type annotation information  see https://github.com/abcwcm/CZI MI mouseZebrafish Assembly: GRCz11 Supplementary files format and content: CellRanger produced filtered feature barcode matrix in H5 format.", "Liver", "Heart cryoinjury or untreated", "Heart ventricles were placed in the ice cold PBS buffer with 1% BSA. post several washes removing blood cells  ventricles were gently cut into small pieces. Tissues were incubated in digestion buffer 0.5 ml HBSS plus 0.26 U/ml Liberase DH [Roche] and 1% sheep serum for 45 minutes at 37\u00b0C while agitating at 750 rpm on an Eppendorf ThermoMixer. Supernatants were collected every 15 min and neutralized with 10% sheep serum  and cells were completely disaggregated by gently pipetting up and down. The dissociated cells were centrifuged at 200 g for 5 min at 4\u00b0C and re suspended in HBSS with 0.05% BSA. The cell suspension 2 ml was then gently added on top of 2 ml HBSS buffer with 7.5% BSA. post spinning down at 200 g for 5 min at 4\u00b0C  the pellet was resuspended in 500 \uf06dl and filtered through a 35 mm strainer.  Whole kidney marrow cells were isolated as described previously. Briefly  kidneys were collected on ice and homogenized immediately in PBS plus 0.05% BSA by using a 10ml syringe with 18 1/2 G. The dissociated cells were washed twice in PBS plus 0.05% BSA and spun down at 300 g for 5 min at 4\u00b0C. Pellets were re suspended in PBS plus 0.05% BSA and filtered through a 35 mm strainer. Livers were collected on ice and incubated in digestion buffer 0.5 ml HBSS plus 0.13 U/ml Liberase DH [Roche] and 1% sheep serum at room temperature. The cell lyses was gently stirred with a Spinbar magnetic stirring bar Bel Art Products  and the supernatant were collected every 5 minutes. The cell lysate was filtered through a 70 mm strainer and centrifuged at 150 g for 3 min at 4\u00b0C. Pellet was resuspended in PBS plus 0.05% BSA and then gently added on top of HBSS buffer with 7.5% BSA. post spinning down at 300 g for 5 min  pellets were washed one more time with PBS plus 0.05% BSA. post centrifuging at 300 g for 5 min  the resuspended cell lyses was filtered through a 35 mm strainer. Pancreatic cells were isolated using the same protocol as liver cells except for the initial spinning down at 300 g for 5 min. All suspended cells were stained with trypan blue and counted using a hemocytometer. Standard library construction protocol using 10x Genomics Chromium Single Cell 3\u2019 GEM  Library & Gel Bead Kit v3  and Chromium Single Cell B Chip Kit.", "Standard zebrafish husbandry protocol.", "tissue:Liver|cell type:whole organ|genotype:wild type|treatment:Heart cryoinjury", "GSM7092954", "GSM7092954: liver Ctrl replicate 1; Danio rerio; RNA Seq", "GSM7092954 r1", "GSM7092954", "1", "Heart ventricles were placed in the ice cold PBS buffer with 1% BSA. post several washes removing blood cells  ventricles were gently cut into small pieces. Tissues were incubated in digestion buffer 0.5 ml HBSS plus 0.26 U/ml Liberase DH [Roche] and 1% sheep serum for 45 minutes at 37\u00b0C while agitating at 750 rpm on an Eppendorf ThermoMixer. Supernatants were collected every 15 min and neutralized with 10% sheep serum  and cells were completely disaggregated by gently pipetting up and down. The dissociated cells were centrifuged at 200 g for 5 min at 4\u00b0C and re suspended in HBSS with 0.05% BSA. The cell suspension 2 ml was then gently added on top of 2 ml HBSS buffer with 7.5% BSA. post spinning down at 200 g for 5 min at 4\u00b0C  the pellet was resuspended in 500 \uf06dl and filtered through a 35 mm strainer.  Whole kidney marrow cells were isolated as described previously. Briefly  kidneys were collected on ice and homogenized immediately in PBS plus 0.05% BSA by using a 10ml syringe with 18 1/2 G. The dissociated cells were washed twice in PBS plus 0.05% BSA and spun down at 300 g for 5 min at 4\u00b0C. Pellets were re suspended in PBS plus 0.05% BSA and filtered through a 35 mm strainer. Livers were collected on ice and incubated in digestion buffer 0.5 ml HBSS plus 0.13 U/ml Liberase DH [Roche] and 1% sheep serum at room temperature. The cell lyses was gently stirred with a Spinbar magnetic stirring bar Bel Art Products  and the supernatant were collected every 5 minutes. The cell lysate was filtered through a 70 mm strainer and centrifuged at 150 g for 3 min at 4\u00b0C. Pellet was resuspended in PBS plus 0.05% BSA and then gently added on top of HBSS buffer with 7.5% BSA. post spinning down at 300 g for 5 min  pellets were washed one more time with PBS plus 0.05% BSA. post centrifuging at 300 g for 5 min  the resuspended cell lyses was filtered through a 35 mm strainer. Pancreatic cells were isolated using the same protocol as liver cells except for the initial spinning down at 300 g for 5 min. All suspended cells were stained with trypan blue and counted using a hemocytometer. Standard library construction protocol using 10x Genomics Chromium Single Cell three prime GEM  Library & Gel Bead Kit v3  and Chromium Single Cell B Chip Kit.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP426909", null, "loader:fastq load.py", "Ctrl-Liver-1_S3_L003_R1_001.fastq.gz Ctrl-Liver-1_S3_L003_R2_001.fastq.gz", "fastq fastq", 4565971178.0, 38694671.0, "GSM7092954 r3", "0:28 1:90", "A:1255012168;C:1078579990;G:1084287166;T:1147999283;N:92571", 28, 90, null, null, 1255012168, 1078579990, 1084287166, 1147999283, 92571, "SRX19646190", "SRS17014000", "SRA1603354", "Applied Bioinformatics Core, Applied Bioinformatics Core, Weill Cornell Medicine", "Applied Bioinformatics Core, Applied Bioinformatics Core, Weill Cornell Medicine", 2, 0.00832, 0.94796, 0.00204, 0.06357, 0.99407, 0.87775, 0.26311, 0.46735, 28, 90, "T", "B", "sc-like readlen", "illumina", "novaseq_era", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "United States", "2023-03-12", "Undetermined", "Undetermined", "Liver", "Liver and Biliary System"], [74487, "SRR23824331", "SRX19646190", "SRS17014000", "SRP426909", "PRJNA943553", "Single cell transcriptomic analyses of the dynamic local and systemic response to cardiac injury in mice and zebrafish [zebrafish]", "GSE227190", "Transcriptome Analysis", "Mice respond to myocardial infarction MI by generating a fibrotic scar in the heart. This is accompanied by systemic inflammation post the MI resolves. In contrast  zebrafish can fully regenerate their heart post heart injury. To further investigate the divergent species' responses to cardiac injury  single cell transcriptomic analyses were performed on the heart  blood  liver  kidney  and pancreatic islets. Overall design: Upon heart cryoinjury  cells from multiple organs heart  liver  pancreas  and whole kidney marrow were isolated for scRNA seq at 1 and 7 xxx post injury together with the uninjured controls.", "parent bioproject:PRJNA943547", "pubmed:39627536", null, "liver Ctrl replicate 1", "GSM7092954", null, "source name:Liver|tissue:Liver|cell type:whole organ|genotype:wild type|treatment:Heart cryoinjury", "liver Ctrl replicate 1", "Demultiplexing  alignment  filtering  and UMI counting was performed with Cell Ranger v6.0.0 using the cellranger count command. Sample integration batch correction  expression value normalization  clustering  cell type annotation and so on were done scater and scran. For more details and the cell type annotation information  see https://github.com/abcwcm/CZI MI mouseZebrafish Assembly: GRCz11 Supplementary files format and content: CellRanger produced filtered feature barcode matrix in H5 format.", "Liver", "Heart cryoinjury or untreated", "Heart ventricles were placed in the ice cold PBS buffer with 1% BSA. post several washes removing blood cells  ventricles were gently cut into small pieces. Tissues were incubated in digestion buffer 0.5 ml HBSS plus 0.26 U/ml Liberase DH [Roche] and 1% sheep serum for 45 minutes at 37\u00b0C while agitating at 750 rpm on an Eppendorf ThermoMixer. Supernatants were collected every 15 min and neutralized with 10% sheep serum  and cells were completely disaggregated by gently pipetting up and down. The dissociated cells were centrifuged at 200 g for 5 min at 4\u00b0C and re suspended in HBSS with 0.05% BSA. The cell suspension 2 ml was then gently added on top of 2 ml HBSS buffer with 7.5% BSA. post spinning down at 200 g for 5 min at 4\u00b0C  the pellet was resuspended in 500 \uf06dl and filtered through a 35 mm strainer.  Whole kidney marrow cells were isolated as described previously. Briefly  kidneys were collected on ice and homogenized immediately in PBS plus 0.05% BSA by using a 10ml syringe with 18 1/2 G. The dissociated cells were washed twice in PBS plus 0.05% BSA and spun down at 300 g for 5 min at 4\u00b0C. Pellets were re suspended in PBS plus 0.05% BSA and filtered through a 35 mm strainer. Livers were collected on ice and incubated in digestion buffer 0.5 ml HBSS plus 0.13 U/ml Liberase DH [Roche] and 1% sheep serum at room temperature. The cell lyses was gently stirred with a Spinbar magnetic stirring bar Bel Art Products  and the supernatant were collected every 5 minutes. The cell lysate was filtered through a 70 mm strainer and centrifuged at 150 g for 3 min at 4\u00b0C. Pellet was resuspended in PBS plus 0.05% BSA and then gently added on top of HBSS buffer with 7.5% BSA. post spinning down at 300 g for 5 min  pellets were washed one more time with PBS plus 0.05% BSA. post centrifuging at 300 g for 5 min  the resuspended cell lyses was filtered through a 35 mm strainer. Pancreatic cells were isolated using the same protocol as liver cells except for the initial spinning down at 300 g for 5 min. All suspended cells were stained with trypan blue and counted using a hemocytometer. Standard library construction protocol using 10x Genomics Chromium Single Cell 3\u2019 GEM  Library & Gel Bead Kit v3  and Chromium Single Cell B Chip Kit.", "Standard zebrafish husbandry protocol.", "tissue:Liver|cell type:whole organ|genotype:wild type|treatment:Heart cryoinjury", "GSM7092954", "GSM7092954: liver Ctrl replicate 1; Danio rerio; RNA Seq", "GSM7092954 r1", "GSM7092954", "1", "Heart ventricles were placed in the ice cold PBS buffer with 1% BSA. post several washes removing blood cells  ventricles were gently cut into small pieces. Tissues were incubated in digestion buffer 0.5 ml HBSS plus 0.26 U/ml Liberase DH [Roche] and 1% sheep serum for 45 minutes at 37\u00b0C while agitating at 750 rpm on an Eppendorf ThermoMixer. Supernatants were collected every 15 min and neutralized with 10% sheep serum  and cells were completely disaggregated by gently pipetting up and down. The dissociated cells were centrifuged at 200 g for 5 min at 4\u00b0C and re suspended in HBSS with 0.05% BSA. The cell suspension 2 ml was then gently added on top of 2 ml HBSS buffer with 7.5% BSA. post spinning down at 200 g for 5 min at 4\u00b0C  the pellet was resuspended in 500 \uf06dl and filtered through a 35 mm strainer.  Whole kidney marrow cells were isolated as described previously. Briefly  kidneys were collected on ice and homogenized immediately in PBS plus 0.05% BSA by using a 10ml syringe with 18 1/2 G. The dissociated cells were washed twice in PBS plus 0.05% BSA and spun down at 300 g for 5 min at 4\u00b0C. Pellets were re suspended in PBS plus 0.05% BSA and filtered through a 35 mm strainer. Livers were collected on ice and incubated in digestion buffer 0.5 ml HBSS plus 0.13 U/ml Liberase DH [Roche] and 1% sheep serum at room temperature. The cell lyses was gently stirred with a Spinbar magnetic stirring bar Bel Art Products  and the supernatant were collected every 5 minutes. The cell lysate was filtered through a 70 mm strainer and centrifuged at 150 g for 3 min at 4\u00b0C. Pellet was resuspended in PBS plus 0.05% BSA and then gently added on top of HBSS buffer with 7.5% BSA. post spinning down at 300 g for 5 min  pellets were washed one more time with PBS plus 0.05% BSA. post centrifuging at 300 g for 5 min  the resuspended cell lyses was filtered through a 35 mm strainer. Pancreatic cells were isolated using the same protocol as liver cells except for the initial spinning down at 300 g for 5 min. All suspended cells were stained with trypan blue and counted using a hemocytometer. Standard library construction protocol using 10x Genomics Chromium Single Cell three prime GEM  Library & Gel Bead Kit v3  and Chromium Single Cell B Chip Kit.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP426909", null, "loader:fastq load.py", "Ctrl-Liver-1_S3_L004_R1_001.fastq.gz Ctrl-Liver-1_S3_L004_R2_001.fastq.gz", "fastq fastq", 4487270016.0, 38027712.0, "GSM7092954 r4", "0:28 1:90", "A:1234826243;C:1058941866;G:1064097171;T:1129318562;N:86174", 28, 90, null, null, 1234826243, 1058941866, 1064097171, 1129318562, 86174, "SRX19646190", "SRS17014000", "SRA1603354", "Applied Bioinformatics Core, Applied Bioinformatics Core, Weill Cornell Medicine", "Applied Bioinformatics Core, Applied Bioinformatics Core, Weill Cornell Medicine", 2, 0.00802, 0.94821, 0.00201, 0.06394, 0.99472, 0.87618, 0.25364, 0.46914, 28, 90, "T", "B", "sc-like readlen", "illumina", "novaseq_era", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "United States", "2023-03-12", "Undetermined", "Undetermined", "Liver", "Liver and Biliary System"], [74512, "SRR23824356", "SRX19646183", "SRS17013993", "SRP426909", "PRJNA943553", "Single cell transcriptomic analyses of the dynamic local and systemic response to cardiac injury in mice and zebrafish [zebrafish]", "GSE227190", "Transcriptome Analysis", "Mice respond to myocardial infarction MI by generating a fibrotic scar in the heart. This is accompanied by systemic inflammation post the MI resolves. In contrast  zebrafish can fully regenerate their heart post heart injury. To further investigate the divergent species' responses to cardiac injury  single cell transcriptomic analyses were performed on the heart  blood  liver  kidney  and pancreatic islets. Overall design: Upon heart cryoinjury  cells from multiple organs heart  liver  pancreas  and whole kidney marrow were isolated for scRNA seq at 1 and 7 xxx post injury together with the uninjured controls.", "parent bioproject:PRJNA943547", "pubmed:39627536", null, "liver 7 dpi replicate 2", "GSM7092947", null, "source name:Liver|tissue:Liver|cell type:whole organ|genotype:wild type|treatment:Heart cryoinjury", "liver 7 dpi replicate 2", "Demultiplexing  alignment  filtering  and UMI counting was performed with Cell Ranger v6.0.0 using the cellranger count command. Sample integration batch correction  expression value normalization  clustering  cell type annotation and so on were done scater and scran. For more details and the cell type annotation information  see https://github.com/abcwcm/CZI MI mouseZebrafish Assembly: GRCz11 Supplementary files format and content: CellRanger produced filtered feature barcode matrix in H5 format.", "Liver", "Heart cryoinjury or untreated", "Heart ventricles were placed in the ice cold PBS buffer with 1% BSA. post several washes removing blood cells  ventricles were gently cut into small pieces. Tissues were incubated in digestion buffer 0.5 ml HBSS plus 0.26 U/ml Liberase DH [Roche] and 1% sheep serum for 45 minutes at 37\u00b0C while agitating at 750 rpm on an Eppendorf ThermoMixer. Supernatants were collected every 15 min and neutralized with 10% sheep serum  and cells were completely disaggregated by gently pipetting up and down. The dissociated cells were centrifuged at 200 g for 5 min at 4\u00b0C and re suspended in HBSS with 0.05% BSA. The cell suspension 2 ml was then gently added on top of 2 ml HBSS buffer with 7.5% BSA. post spinning down at 200 g for 5 min at 4\u00b0C  the pellet was resuspended in 500 \uf06dl and filtered through a 35 mm strainer.  Whole kidney marrow cells were isolated as described previously. Briefly  kidneys were collected on ice and homogenized immediately in PBS plus 0.05% BSA by using a 10ml syringe with 18 1/2 G. The dissociated cells were washed twice in PBS plus 0.05% BSA and spun down at 300 g for 5 min at 4\u00b0C. Pellets were re suspended in PBS plus 0.05% BSA and filtered through a 35 mm strainer. Livers were collected on ice and incubated in digestion buffer 0.5 ml HBSS plus 0.13 U/ml Liberase DH [Roche] and 1% sheep serum at room temperature. The cell lyses was gently stirred with a Spinbar magnetic stirring bar Bel Art Products  and the supernatant were collected every 5 minutes. The cell lysate was filtered through a 70 mm strainer and centrifuged at 150 g for 3 min at 4\u00b0C. Pellet was resuspended in PBS plus 0.05% BSA and then gently added on top of HBSS buffer with 7.5% BSA. post spinning down at 300 g for 5 min  pellets were washed one more time with PBS plus 0.05% BSA. post centrifuging at 300 g for 5 min  the resuspended cell lyses was filtered through a 35 mm strainer. Pancreatic cells were isolated using the same protocol as liver cells except for the initial spinning down at 300 g for 5 min. All suspended cells were stained with trypan blue and counted using a hemocytometer. Standard library construction protocol using 10x Genomics Chromium Single Cell 3\u2019 GEM  Library & Gel Bead Kit v3  and Chromium Single Cell B Chip Kit.", "Standard zebrafish husbandry protocol.", "tissue:Liver|cell type:whole organ|genotype:wild type|treatment:Heart cryoinjury", "GSM7092947", "GSM7092947: liver 7 dpi replicate 2; Danio rerio; RNA Seq", "GSM7092947 r1", "GSM7092947", "1", "Heart ventricles were placed in the ice cold PBS buffer with 1% BSA. post several washes removing blood cells  ventricles were gently cut into small pieces. Tissues were incubated in digestion buffer 0.5 ml HBSS plus 0.26 U/ml Liberase DH [Roche] and 1% sheep serum for 45 minutes at 37\u00b0C while agitating at 750 rpm on an Eppendorf ThermoMixer. Supernatants were collected every 15 min and neutralized with 10% sheep serum  and cells were completely disaggregated by gently pipetting up and down. The dissociated cells were centrifuged at 200 g for 5 min at 4\u00b0C and re suspended in HBSS with 0.05% BSA. The cell suspension 2 ml was then gently added on top of 2 ml HBSS buffer with 7.5% BSA. post spinning down at 200 g for 5 min at 4\u00b0C  the pellet was resuspended in 500 \uf06dl and filtered through a 35 mm strainer.  Whole kidney marrow cells were isolated as described previously. Briefly  kidneys were collected on ice and homogenized immediately in PBS plus 0.05% BSA by using a 10ml syringe with 18 1/2 G. The dissociated cells were washed twice in PBS plus 0.05% BSA and spun down at 300 g for 5 min at 4\u00b0C. Pellets were re suspended in PBS plus 0.05% BSA and filtered through a 35 mm strainer. Livers were collected on ice and incubated in digestion buffer 0.5 ml HBSS plus 0.13 U/ml Liberase DH [Roche] and 1% sheep serum at room temperature. The cell lyses was gently stirred with a Spinbar magnetic stirring bar Bel Art Products  and the supernatant were collected every 5 minutes. The cell lysate was filtered through a 70 mm strainer and centrifuged at 150 g for 3 min at 4\u00b0C. Pellet was resuspended in PBS plus 0.05% BSA and then gently added on top of HBSS buffer with 7.5% BSA. post spinning down at 300 g for 5 min  pellets were washed one more time with PBS plus 0.05% BSA. post centrifuging at 300 g for 5 min  the resuspended cell lyses was filtered through a 35 mm strainer. Pancreatic cells were isolated using the same protocol as liver cells except for the initial spinning down at 300 g for 5 min. All suspended cells were stained with trypan blue and counted using a hemocytometer. Standard library construction protocol using 10x Genomics Chromium Single Cell three prime GEM  Library & Gel Bead Kit v3  and Chromium Single Cell B Chip Kit.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP426909", null, "loader:fastq load.py", "7dpi-Liver-2_S23_L001_R1_001.fastq.gz 7dpi-Liver-2_S23_L001_R2_001.fastq.gz", "fastq fastq", 2901696228.0, 24590646.0, "GSM7092947 r1", "0:28 1:90", "A:800794183;C:688235467;G:699322734;T:713278095;N:65749", 28, 90, null, null, 800794183, 688235467, 699322734, 713278095, 65749, "SRX19646183", "SRS17013993", "SRA1603354", "Applied Bioinformatics Core, Applied Bioinformatics Core, Weill Cornell Medicine", "Applied Bioinformatics Core, Applied Bioinformatics Core, Weill Cornell Medicine", 2, 0.01367, 0.94349, 0.00359, 0.06447, 0.99371, 0.8983, 0.35863, 0.50961, 28, 90, "T", "B", "sc-like readlen", "illumina", "novaseq_era", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "United States", "2023-03-12", "Undetermined", "Undetermined", "Liver", "Liver and Biliary System"], [74513, "SRR23824357", "SRX19646183", "SRS17013993", "SRP426909", "PRJNA943553", "Single cell transcriptomic analyses of the dynamic local and systemic response to cardiac injury in mice and zebrafish [zebrafish]", "GSE227190", "Transcriptome Analysis", "Mice respond to myocardial infarction MI by generating a fibrotic scar in the heart. This is accompanied by systemic inflammation post the MI resolves. In contrast  zebrafish can fully regenerate their heart post heart injury. To further investigate the divergent species' responses to cardiac injury  single cell transcriptomic analyses were performed on the heart  blood  liver  kidney  and pancreatic islets. Overall design: Upon heart cryoinjury  cells from multiple organs heart  liver  pancreas  and whole kidney marrow were isolated for scRNA seq at 1 and 7 xxx post injury together with the uninjured controls.", "parent bioproject:PRJNA943547", "pubmed:39627536", null, "liver 7 dpi replicate 2", "GSM7092947", null, "source name:Liver|tissue:Liver|cell type:whole organ|genotype:wild type|treatment:Heart cryoinjury", "liver 7 dpi replicate 2", "Demultiplexing  alignment  filtering  and UMI counting was performed with Cell Ranger v6.0.0 using the cellranger count command. Sample integration batch correction  expression value normalization  clustering  cell type annotation and so on were done scater and scran. For more details and the cell type annotation information  see https://github.com/abcwcm/CZI MI mouseZebrafish Assembly: GRCz11 Supplementary files format and content: CellRanger produced filtered feature barcode matrix in H5 format.", "Liver", "Heart cryoinjury or untreated", "Heart ventricles were placed in the ice cold PBS buffer with 1% BSA. post several washes removing blood cells  ventricles were gently cut into small pieces. Tissues were incubated in digestion buffer 0.5 ml HBSS plus 0.26 U/ml Liberase DH [Roche] and 1% sheep serum for 45 minutes at 37\u00b0C while agitating at 750 rpm on an Eppendorf ThermoMixer. Supernatants were collected every 15 min and neutralized with 10% sheep serum  and cells were completely disaggregated by gently pipetting up and down. The dissociated cells were centrifuged at 200 g for 5 min at 4\u00b0C and re suspended in HBSS with 0.05% BSA. The cell suspension 2 ml was then gently added on top of 2 ml HBSS buffer with 7.5% BSA. post spinning down at 200 g for 5 min at 4\u00b0C  the pellet was resuspended in 500 \uf06dl and filtered through a 35 mm strainer.  Whole kidney marrow cells were isolated as described previously. Briefly  kidneys were collected on ice and homogenized immediately in PBS plus 0.05% BSA by using a 10ml syringe with 18 1/2 G. The dissociated cells were washed twice in PBS plus 0.05% BSA and spun down at 300 g for 5 min at 4\u00b0C. Pellets were re suspended in PBS plus 0.05% BSA and filtered through a 35 mm strainer. Livers were collected on ice and incubated in digestion buffer 0.5 ml HBSS plus 0.13 U/ml Liberase DH [Roche] and 1% sheep serum at room temperature. The cell lyses was gently stirred with a Spinbar magnetic stirring bar Bel Art Products  and the supernatant were collected every 5 minutes. The cell lysate was filtered through a 70 mm strainer and centrifuged at 150 g for 3 min at 4\u00b0C. Pellet was resuspended in PBS plus 0.05% BSA and then gently added on top of HBSS buffer with 7.5% BSA. post spinning down at 300 g for 5 min  pellets were washed one more time with PBS plus 0.05% BSA. post centrifuging at 300 g for 5 min  the resuspended cell lyses was filtered through a 35 mm strainer. Pancreatic cells were isolated using the same protocol as liver cells except for the initial spinning down at 300 g for 5 min. All suspended cells were stained with trypan blue and counted using a hemocytometer. Standard library construction protocol using 10x Genomics Chromium Single Cell 3\u2019 GEM  Library & Gel Bead Kit v3  and Chromium Single Cell B Chip Kit.", "Standard zebrafish husbandry protocol.", "tissue:Liver|cell type:whole organ|genotype:wild type|treatment:Heart cryoinjury", "GSM7092947", "GSM7092947: liver 7 dpi replicate 2; Danio rerio; RNA Seq", "GSM7092947 r1", "GSM7092947", "1", "Heart ventricles were placed in the ice cold PBS buffer with 1% BSA. post several washes removing blood cells  ventricles were gently cut into small pieces. Tissues were incubated in digestion buffer 0.5 ml HBSS plus 0.26 U/ml Liberase DH [Roche] and 1% sheep serum for 45 minutes at 37\u00b0C while agitating at 750 rpm on an Eppendorf ThermoMixer. Supernatants were collected every 15 min and neutralized with 10% sheep serum  and cells were completely disaggregated by gently pipetting up and down. The dissociated cells were centrifuged at 200 g for 5 min at 4\u00b0C and re suspended in HBSS with 0.05% BSA. The cell suspension 2 ml was then gently added on top of 2 ml HBSS buffer with 7.5% BSA. post spinning down at 200 g for 5 min at 4\u00b0C  the pellet was resuspended in 500 \uf06dl and filtered through a 35 mm strainer.  Whole kidney marrow cells were isolated as described previously. Briefly  kidneys were collected on ice and homogenized immediately in PBS plus 0.05% BSA by using a 10ml syringe with 18 1/2 G. The dissociated cells were washed twice in PBS plus 0.05% BSA and spun down at 300 g for 5 min at 4\u00b0C. Pellets were re suspended in PBS plus 0.05% BSA and filtered through a 35 mm strainer. Livers were collected on ice and incubated in digestion buffer 0.5 ml HBSS plus 0.13 U/ml Liberase DH [Roche] and 1% sheep serum at room temperature. The cell lyses was gently stirred with a Spinbar magnetic stirring bar Bel Art Products  and the supernatant were collected every 5 minutes. The cell lysate was filtered through a 70 mm strainer and centrifuged at 150 g for 3 min at 4\u00b0C. Pellet was resuspended in PBS plus 0.05% BSA and then gently added on top of HBSS buffer with 7.5% BSA. post spinning down at 300 g for 5 min  pellets were washed one more time with PBS plus 0.05% BSA. post centrifuging at 300 g for 5 min  the resuspended cell lyses was filtered through a 35 mm strainer. Pancreatic cells were isolated using the same protocol as liver cells except for the initial spinning down at 300 g for 5 min. All suspended cells were stained with trypan blue and counted using a hemocytometer. Standard library construction protocol using 10x Genomics Chromium Single Cell three prime GEM  Library & Gel Bead Kit v3  and Chromium Single Cell B Chip Kit.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP426909", null, "loader:fastq load.py", "7dpi-Liver-2_S23_L002_R1_001.fastq.gz 7dpi-Liver-2_S23_L002_R2_001.fastq.gz", "fastq fastq", 2911402436.0, 24672902.0, "GSM7092947 r2", "0:28 1:90", "A:803250356;C:690504502;G:701686700;T:715898105;N:62773", 28, 90, null, null, 803250356, 690504502, 701686700, 715898105, 62773, "SRX19646183", "SRS17013993", "SRA1603354", "Applied Bioinformatics Core, Applied Bioinformatics Core, Weill Cornell Medicine", "Applied Bioinformatics Core, Applied Bioinformatics Core, Weill Cornell Medicine", 2, 0.01353, 0.94324, 0.00375, 0.06448, 0.9934, 0.89512, 0.36797, 0.50133, 28, 90, "T", "B", "sc-like readlen", "illumina", "novaseq_era", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "United States", "2023-03-12", "Undetermined", "Undetermined", "Liver", "Liver and Biliary System"], [74514, "SRR23824358", "SRX19646183", "SRS17013993", "SRP426909", "PRJNA943553", "Single cell transcriptomic analyses of the dynamic local and systemic response to cardiac injury in mice and zebrafish [zebrafish]", "GSE227190", "Transcriptome Analysis", "Mice respond to myocardial infarction MI by generating a fibrotic scar in the heart. This is accompanied by systemic inflammation post the MI resolves. In contrast  zebrafish can fully regenerate their heart post heart injury. To further investigate the divergent species' responses to cardiac injury  single cell transcriptomic analyses were performed on the heart  blood  liver  kidney  and pancreatic islets. Overall design: Upon heart cryoinjury  cells from multiple organs heart  liver  pancreas  and whole kidney marrow were isolated for scRNA seq at 1 and 7 xxx post injury together with the uninjured controls.", "parent bioproject:PRJNA943547", "pubmed:39627536", null, "liver 7 dpi replicate 2", "GSM7092947", null, "source name:Liver|tissue:Liver|cell type:whole organ|genotype:wild type|treatment:Heart cryoinjury", "liver 7 dpi replicate 2", "Demultiplexing  alignment  filtering  and UMI counting was performed with Cell Ranger v6.0.0 using the cellranger count command. Sample integration batch correction  expression value normalization  clustering  cell type annotation and so on were done scater and scran. For more details and the cell type annotation information  see https://github.com/abcwcm/CZI MI mouseZebrafish Assembly: GRCz11 Supplementary files format and content: CellRanger produced filtered feature barcode matrix in H5 format.", "Liver", "Heart cryoinjury or untreated", "Heart ventricles were placed in the ice cold PBS buffer with 1% BSA. post several washes removing blood cells  ventricles were gently cut into small pieces. Tissues were incubated in digestion buffer 0.5 ml HBSS plus 0.26 U/ml Liberase DH [Roche] and 1% sheep serum for 45 minutes at 37\u00b0C while agitating at 750 rpm on an Eppendorf ThermoMixer. Supernatants were collected every 15 min and neutralized with 10% sheep serum  and cells were completely disaggregated by gently pipetting up and down. The dissociated cells were centrifuged at 200 g for 5 min at 4\u00b0C and re suspended in HBSS with 0.05% BSA. The cell suspension 2 ml was then gently added on top of 2 ml HBSS buffer with 7.5% BSA. post spinning down at 200 g for 5 min at 4\u00b0C  the pellet was resuspended in 500 \uf06dl and filtered through a 35 mm strainer.  Whole kidney marrow cells were isolated as described previously. Briefly  kidneys were collected on ice and homogenized immediately in PBS plus 0.05% BSA by using a 10ml syringe with 18 1/2 G. The dissociated cells were washed twice in PBS plus 0.05% BSA and spun down at 300 g for 5 min at 4\u00b0C. Pellets were re suspended in PBS plus 0.05% BSA and filtered through a 35 mm strainer. Livers were collected on ice and incubated in digestion buffer 0.5 ml HBSS plus 0.13 U/ml Liberase DH [Roche] and 1% sheep serum at room temperature. The cell lyses was gently stirred with a Spinbar magnetic stirring bar Bel Art Products  and the supernatant were collected every 5 minutes. The cell lysate was filtered through a 70 mm strainer and centrifuged at 150 g for 3 min at 4\u00b0C. Pellet was resuspended in PBS plus 0.05% BSA and then gently added on top of HBSS buffer with 7.5% BSA. post spinning down at 300 g for 5 min  pellets were washed one more time with PBS plus 0.05% BSA. post centrifuging at 300 g for 5 min  the resuspended cell lyses was filtered through a 35 mm strainer. Pancreatic cells were isolated using the same protocol as liver cells except for the initial spinning down at 300 g for 5 min. All suspended cells were stained with trypan blue and counted using a hemocytometer. Standard library construction protocol using 10x Genomics Chromium Single Cell 3\u2019 GEM  Library & Gel Bead Kit v3  and Chromium Single Cell B Chip Kit.", "Standard zebrafish husbandry protocol.", "tissue:Liver|cell type:whole organ|genotype:wild type|treatment:Heart cryoinjury", "GSM7092947", "GSM7092947: liver 7 dpi replicate 2; Danio rerio; RNA Seq", "GSM7092947 r1", "GSM7092947", "1", "Heart ventricles were placed in the ice cold PBS buffer with 1% BSA. post several washes removing blood cells  ventricles were gently cut into small pieces. Tissues were incubated in digestion buffer 0.5 ml HBSS plus 0.26 U/ml Liberase DH [Roche] and 1% sheep serum for 45 minutes at 37\u00b0C while agitating at 750 rpm on an Eppendorf ThermoMixer. Supernatants were collected every 15 min and neutralized with 10% sheep serum  and cells were completely disaggregated by gently pipetting up and down. The dissociated cells were centrifuged at 200 g for 5 min at 4\u00b0C and re suspended in HBSS with 0.05% BSA. The cell suspension 2 ml was then gently added on top of 2 ml HBSS buffer with 7.5% BSA. post spinning down at 200 g for 5 min at 4\u00b0C  the pellet was resuspended in 500 \uf06dl and filtered through a 35 mm strainer.  Whole kidney marrow cells were isolated as described previously. Briefly  kidneys were collected on ice and homogenized immediately in PBS plus 0.05% BSA by using a 10ml syringe with 18 1/2 G. The dissociated cells were washed twice in PBS plus 0.05% BSA and spun down at 300 g for 5 min at 4\u00b0C. Pellets were re suspended in PBS plus 0.05% BSA and filtered through a 35 mm strainer. Livers were collected on ice and incubated in digestion buffer 0.5 ml HBSS plus 0.13 U/ml Liberase DH [Roche] and 1% sheep serum at room temperature. The cell lyses was gently stirred with a Spinbar magnetic stirring bar Bel Art Products  and the supernatant were collected every 5 minutes. The cell lysate was filtered through a 70 mm strainer and centrifuged at 150 g for 3 min at 4\u00b0C. Pellet was resuspended in PBS plus 0.05% BSA and then gently added on top of HBSS buffer with 7.5% BSA. post spinning down at 300 g for 5 min  pellets were washed one more time with PBS plus 0.05% BSA. post centrifuging at 300 g for 5 min  the resuspended cell lyses was filtered through a 35 mm strainer. Pancreatic cells were isolated using the same protocol as liver cells except for the initial spinning down at 300 g for 5 min. All suspended cells were stained with trypan blue and counted using a hemocytometer. Standard library construction protocol using 10x Genomics Chromium Single Cell three prime GEM  Library & Gel Bead Kit v3  and Chromium Single Cell B Chip Kit.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP426909", null, "loader:fastq load.py", "7dpi-Liver-2_S23_L003_R1_001.fastq.gz 7dpi-Liver-2_S23_L003_R2_001.fastq.gz", "fastq fastq", 2952479180.0, 25021010.0, "GSM7092947 r3", "0:28 1:90", "A:814071655;C:700696343;G:712162705;T:725488870;N:59607", 28, 90, null, null, 814071655, 700696343, 712162705, 725488870, 59607, "SRX19646183", "SRS17013993", "SRA1603354", "Applied Bioinformatics Core, Applied Bioinformatics Core, Weill Cornell Medicine", "Applied Bioinformatics Core, Applied Bioinformatics Core, Weill Cornell Medicine", 2, 0.01352, 0.9422, 0.00377, 0.06446, 0.99383, 0.89858, 0.3405, 0.51469, 28, 90, "T", "B", "sc-like readlen", "illumina", "novaseq_era", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "United States", "2023-03-12", "Undetermined", "Undetermined", "Liver", "Liver and Biliary System"], [74515, "SRR23824359", "SRX19646183", "SRS17013993", "SRP426909", "PRJNA943553", "Single cell transcriptomic analyses of the dynamic local and systemic response to cardiac injury in mice and zebrafish [zebrafish]", "GSE227190", "Transcriptome Analysis", "Mice respond to myocardial infarction MI by generating a fibrotic scar in the heart. This is accompanied by systemic inflammation post the MI resolves. In contrast  zebrafish can fully regenerate their heart post heart injury. To further investigate the divergent species' responses to cardiac injury  single cell transcriptomic analyses were performed on the heart  blood  liver  kidney  and pancreatic islets. Overall design: Upon heart cryoinjury  cells from multiple organs heart  liver  pancreas  and whole kidney marrow were isolated for scRNA seq at 1 and 7 xxx post injury together with the uninjured controls.", "parent bioproject:PRJNA943547", "pubmed:39627536", null, "liver 7 dpi replicate 2", "GSM7092947", null, "source name:Liver|tissue:Liver|cell type:whole organ|genotype:wild type|treatment:Heart cryoinjury", "liver 7 dpi replicate 2", "Demultiplexing  alignment  filtering  and UMI counting was performed with Cell Ranger v6.0.0 using the cellranger count command. Sample integration batch correction  expression value normalization  clustering  cell type annotation and so on were done scater and scran. For more details and the cell type annotation information  see https://github.com/abcwcm/CZI MI mouseZebrafish Assembly: GRCz11 Supplementary files format and content: CellRanger produced filtered feature barcode matrix in H5 format.", "Liver", "Heart cryoinjury or untreated", "Heart ventricles were placed in the ice cold PBS buffer with 1% BSA. post several washes removing blood cells  ventricles were gently cut into small pieces. Tissues were incubated in digestion buffer 0.5 ml HBSS plus 0.26 U/ml Liberase DH [Roche] and 1% sheep serum for 45 minutes at 37\u00b0C while agitating at 750 rpm on an Eppendorf ThermoMixer. Supernatants were collected every 15 min and neutralized with 10% sheep serum  and cells were completely disaggregated by gently pipetting up and down. The dissociated cells were centrifuged at 200 g for 5 min at 4\u00b0C and re suspended in HBSS with 0.05% BSA. The cell suspension 2 ml was then gently added on top of 2 ml HBSS buffer with 7.5% BSA. post spinning down at 200 g for 5 min at 4\u00b0C  the pellet was resuspended in 500 \uf06dl and filtered through a 35 mm strainer.  Whole kidney marrow cells were isolated as described previously. Briefly  kidneys were collected on ice and homogenized immediately in PBS plus 0.05% BSA by using a 10ml syringe with 18 1/2 G. The dissociated cells were washed twice in PBS plus 0.05% BSA and spun down at 300 g for 5 min at 4\u00b0C. Pellets were re suspended in PBS plus 0.05% BSA and filtered through a 35 mm strainer. Livers were collected on ice and incubated in digestion buffer 0.5 ml HBSS plus 0.13 U/ml Liberase DH [Roche] and 1% sheep serum at room temperature. The cell lyses was gently stirred with a Spinbar magnetic stirring bar Bel Art Products  and the supernatant were collected every 5 minutes. The cell lysate was filtered through a 70 mm strainer and centrifuged at 150 g for 3 min at 4\u00b0C. Pellet was resuspended in PBS plus 0.05% BSA and then gently added on top of HBSS buffer with 7.5% BSA. post spinning down at 300 g for 5 min  pellets were washed one more time with PBS plus 0.05% BSA. post centrifuging at 300 g for 5 min  the resuspended cell lyses was filtered through a 35 mm strainer. Pancreatic cells were isolated using the same protocol as liver cells except for the initial spinning down at 300 g for 5 min. All suspended cells were stained with trypan blue and counted using a hemocytometer. Standard library construction protocol using 10x Genomics Chromium Single Cell 3\u2019 GEM  Library & Gel Bead Kit v3  and Chromium Single Cell B Chip Kit.", "Standard zebrafish husbandry protocol.", "tissue:Liver|cell type:whole organ|genotype:wild type|treatment:Heart cryoinjury", "GSM7092947", "GSM7092947: liver 7 dpi replicate 2; Danio rerio; RNA Seq", "GSM7092947 r1", "GSM7092947", "1", "Heart ventricles were placed in the ice cold PBS buffer with 1% BSA. post several washes removing blood cells  ventricles were gently cut into small pieces. Tissues were incubated in digestion buffer 0.5 ml HBSS plus 0.26 U/ml Liberase DH [Roche] and 1% sheep serum for 45 minutes at 37\u00b0C while agitating at 750 rpm on an Eppendorf ThermoMixer. Supernatants were collected every 15 min and neutralized with 10% sheep serum  and cells were completely disaggregated by gently pipetting up and down. The dissociated cells were centrifuged at 200 g for 5 min at 4\u00b0C and re suspended in HBSS with 0.05% BSA. The cell suspension 2 ml was then gently added on top of 2 ml HBSS buffer with 7.5% BSA. post spinning down at 200 g for 5 min at 4\u00b0C  the pellet was resuspended in 500 \uf06dl and filtered through a 35 mm strainer.  Whole kidney marrow cells were isolated as described previously. Briefly  kidneys were collected on ice and homogenized immediately in PBS plus 0.05% BSA by using a 10ml syringe with 18 1/2 G. The dissociated cells were washed twice in PBS plus 0.05% BSA and spun down at 300 g for 5 min at 4\u00b0C. Pellets were re suspended in PBS plus 0.05% BSA and filtered through a 35 mm strainer. Livers were collected on ice and incubated in digestion buffer 0.5 ml HBSS plus 0.13 U/ml Liberase DH [Roche] and 1% sheep serum at room temperature. The cell lyses was gently stirred with a Spinbar magnetic stirring bar Bel Art Products  and the supernatant were collected every 5 minutes. The cell lysate was filtered through a 70 mm strainer and centrifuged at 150 g for 3 min at 4\u00b0C. Pellet was resuspended in PBS plus 0.05% BSA and then gently added on top of HBSS buffer with 7.5% BSA. post spinning down at 300 g for 5 min  pellets were washed one more time with PBS plus 0.05% BSA. post centrifuging at 300 g for 5 min  the resuspended cell lyses was filtered through a 35 mm strainer. Pancreatic cells were isolated using the same protocol as liver cells except for the initial spinning down at 300 g for 5 min. All suspended cells were stained with trypan blue and counted using a hemocytometer. Standard library construction protocol using 10x Genomics Chromium Single Cell three prime GEM  Library & Gel Bead Kit v3  and Chromium Single Cell B Chip Kit.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP426909", null, "loader:fastq load.py", "7dpi-Liver-2_S23_L004_R1_001.fastq.gz 7dpi-Liver-2_S23_L004_R2_001.fastq.gz", "fastq fastq", 2888880956.0, 24482042.0, "GSM7092947 r4", "0:28 1:90", "A:797569254;C:684817990;G:695605635;T:710833096;N:54981", 28, 90, null, null, 797569254, 684817990, 695605635, 710833096, 54981, "SRX19646183", "SRS17013993", "SRA1603354", "Applied Bioinformatics Core, Applied Bioinformatics Core, Weill Cornell Medicine", "Applied Bioinformatics Core, Applied Bioinformatics Core, Weill Cornell Medicine", 2, 0.01297, 0.94356, 0.00358, 0.0658, 0.99366, 0.89406, 0.37493, 0.50579, 28, 90, "T", "B", "sc-like readlen", "illumina", "novaseq_era", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "United States", "2023-03-12", "Undetermined", "Undetermined", "Liver", "Liver and Biliary System"], [74516, "SRR23824360", "SRX19646182", "SRS17013992", "SRP426909", "PRJNA943553", "Single cell transcriptomic analyses of the dynamic local and systemic response to cardiac injury in mice and zebrafish [zebrafish]", "GSE227190", "Transcriptome Analysis", "Mice respond to myocardial infarction MI by generating a fibrotic scar in the heart. This is accompanied by systemic inflammation post the MI resolves. In contrast  zebrafish can fully regenerate their heart post heart injury. To further investigate the divergent species' responses to cardiac injury  single cell transcriptomic analyses were performed on the heart  blood  liver  kidney  and pancreatic islets. Overall design: Upon heart cryoinjury  cells from multiple organs heart  liver  pancreas  and whole kidney marrow were isolated for scRNA seq at 1 and 7 xxx post injury together with the uninjured controls.", "parent bioproject:PRJNA943547", "pubmed:39627536", null, "liver 7 dpi replicate 1", "GSM7092946", null, "source name:Liver|tissue:Liver|cell type:whole organ|genotype:wild type|treatment:Heart cryoinjury", "liver 7 dpi replicate 1", "Demultiplexing  alignment  filtering  and UMI counting was performed with Cell Ranger v6.0.0 using the cellranger count command. Sample integration batch correction  expression value normalization  clustering  cell type annotation and so on were done scater and scran. For more details and the cell type annotation information  see https://github.com/abcwcm/CZI MI mouseZebrafish Assembly: GRCz11 Supplementary files format and content: CellRanger produced filtered feature barcode matrix in H5 format.", "Liver", "Heart cryoinjury or untreated", "Heart ventricles were placed in the ice cold PBS buffer with 1% BSA. post several washes removing blood cells  ventricles were gently cut into small pieces. Tissues were incubated in digestion buffer 0.5 ml HBSS plus 0.26 U/ml Liberase DH [Roche] and 1% sheep serum for 45 minutes at 37\u00b0C while agitating at 750 rpm on an Eppendorf ThermoMixer. Supernatants were collected every 15 min and neutralized with 10% sheep serum  and cells were completely disaggregated by gently pipetting up and down. The dissociated cells were centrifuged at 200 g for 5 min at 4\u00b0C and re suspended in HBSS with 0.05% BSA. The cell suspension 2 ml was then gently added on top of 2 ml HBSS buffer with 7.5% BSA. post spinning down at 200 g for 5 min at 4\u00b0C  the pellet was resuspended in 500 \uf06dl and filtered through a 35 mm strainer.  Whole kidney marrow cells were isolated as described previously. Briefly  kidneys were collected on ice and homogenized immediately in PBS plus 0.05% BSA by using a 10ml syringe with 18 1/2 G. The dissociated cells were washed twice in PBS plus 0.05% BSA and spun down at 300 g for 5 min at 4\u00b0C. Pellets were re suspended in PBS plus 0.05% BSA and filtered through a 35 mm strainer. Livers were collected on ice and incubated in digestion buffer 0.5 ml HBSS plus 0.13 U/ml Liberase DH [Roche] and 1% sheep serum at room temperature. The cell lyses was gently stirred with a Spinbar magnetic stirring bar Bel Art Products  and the supernatant were collected every 5 minutes. The cell lysate was filtered through a 70 mm strainer and centrifuged at 150 g for 3 min at 4\u00b0C. Pellet was resuspended in PBS plus 0.05% BSA and then gently added on top of HBSS buffer with 7.5% BSA. post spinning down at 300 g for 5 min  pellets were washed one more time with PBS plus 0.05% BSA. post centrifuging at 300 g for 5 min  the resuspended cell lyses was filtered through a 35 mm strainer. Pancreatic cells were isolated using the same protocol as liver cells except for the initial spinning down at 300 g for 5 min. All suspended cells were stained with trypan blue and counted using a hemocytometer. Standard library construction protocol using 10x Genomics Chromium Single Cell 3\u2019 GEM  Library & Gel Bead Kit v3  and Chromium Single Cell B Chip Kit.", "Standard zebrafish husbandry protocol.", "tissue:Liver|cell type:whole organ|genotype:wild type|treatment:Heart cryoinjury", "GSM7092946", "GSM7092946: liver 7 dpi replicate 1; Danio rerio; RNA Seq", "GSM7092946 r1", "GSM7092946", "1", "Heart ventricles were placed in the ice cold PBS buffer with 1% BSA. post several washes removing blood cells  ventricles were gently cut into small pieces. Tissues were incubated in digestion buffer 0.5 ml HBSS plus 0.26 U/ml Liberase DH [Roche] and 1% sheep serum for 45 minutes at 37\u00b0C while agitating at 750 rpm on an Eppendorf ThermoMixer. Supernatants were collected every 15 min and neutralized with 10% sheep serum  and cells were completely disaggregated by gently pipetting up and down. The dissociated cells were centrifuged at 200 g for 5 min at 4\u00b0C and re suspended in HBSS with 0.05% BSA. The cell suspension 2 ml was then gently added on top of 2 ml HBSS buffer with 7.5% BSA. post spinning down at 200 g for 5 min at 4\u00b0C  the pellet was resuspended in 500 \uf06dl and filtered through a 35 mm strainer.  Whole kidney marrow cells were isolated as described previously. Briefly  kidneys were collected on ice and homogenized immediately in PBS plus 0.05% BSA by using a 10ml syringe with 18 1/2 G. The dissociated cells were washed twice in PBS plus 0.05% BSA and spun down at 300 g for 5 min at 4\u00b0C. Pellets were re suspended in PBS plus 0.05% BSA and filtered through a 35 mm strainer. Livers were collected on ice and incubated in digestion buffer 0.5 ml HBSS plus 0.13 U/ml Liberase DH [Roche] and 1% sheep serum at room temperature. The cell lyses was gently stirred with a Spinbar magnetic stirring bar Bel Art Products  and the supernatant were collected every 5 minutes. The cell lysate was filtered through a 70 mm strainer and centrifuged at 150 g for 3 min at 4\u00b0C. Pellet was resuspended in PBS plus 0.05% BSA and then gently added on top of HBSS buffer with 7.5% BSA. post spinning down at 300 g for 5 min  pellets were washed one more time with PBS plus 0.05% BSA. post centrifuging at 300 g for 5 min  the resuspended cell lyses was filtered through a 35 mm strainer. Pancreatic cells were isolated using the same protocol as liver cells except for the initial spinning down at 300 g for 5 min. All suspended cells were stained with trypan blue and counted using a hemocytometer. Standard library construction protocol using 10x Genomics Chromium Single Cell three prime GEM  Library & Gel Bead Kit v3  and Chromium Single Cell B Chip Kit.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP426909", null, "loader:fastq load.py", "7dpi-Liver-1_S19_L001_R1_001.fastq.gz 7dpi-Liver-1_S19_L001_R2_001.fastq.gz", "fastq fastq", 3336256244.0, 28273358.0, "GSM7092946 r1", "0:28 1:90", "A:928141930;C:786200774;G:796573600;T:825264837;N:75103", 28, 90, null, null, 928141930, 786200774, 796573600, 825264837, 75103, "SRX19646182", "SRS17013992", "SRA1603354", "Applied Bioinformatics Core, Applied Bioinformatics Core, Weill Cornell Medicine", "Applied Bioinformatics Core, Applied Bioinformatics Core, Weill Cornell Medicine", 2, 0.01265, 0.9442, 0.00339, 0.0656, 0.9933, 0.88477, 0.39633, 0.57337, 28, 90, "T", "B", "sc-like readlen", "illumina", "novaseq_era", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "United States", "2023-03-12", "Undetermined", "Undetermined", "Liver", "Liver and Biliary System"], [74517, "SRR23824361", "SRX19646182", "SRS17013992", "SRP426909", "PRJNA943553", "Single cell transcriptomic analyses of the dynamic local and systemic response to cardiac injury in mice and zebrafish [zebrafish]", "GSE227190", "Transcriptome Analysis", "Mice respond to myocardial infarction MI by generating a fibrotic scar in the heart. This is accompanied by systemic inflammation post the MI resolves. In contrast  zebrafish can fully regenerate their heart post heart injury. To further investigate the divergent species' responses to cardiac injury  single cell transcriptomic analyses were performed on the heart  blood  liver  kidney  and pancreatic islets. Overall design: Upon heart cryoinjury  cells from multiple organs heart  liver  pancreas  and whole kidney marrow were isolated for scRNA seq at 1 and 7 xxx post injury together with the uninjured controls.", "parent bioproject:PRJNA943547", "pubmed:39627536", null, "liver 7 dpi replicate 1", "GSM7092946", null, "source name:Liver|tissue:Liver|cell type:whole organ|genotype:wild type|treatment:Heart cryoinjury", "liver 7 dpi replicate 1", "Demultiplexing  alignment  filtering  and UMI counting was performed with Cell Ranger v6.0.0 using the cellranger count command. Sample integration batch correction  expression value normalization  clustering  cell type annotation and so on were done scater and scran. For more details and the cell type annotation information  see https://github.com/abcwcm/CZI MI mouseZebrafish Assembly: GRCz11 Supplementary files format and content: CellRanger produced filtered feature barcode matrix in H5 format.", "Liver", "Heart cryoinjury or untreated", "Heart ventricles were placed in the ice cold PBS buffer with 1% BSA. post several washes removing blood cells  ventricles were gently cut into small pieces. Tissues were incubated in digestion buffer 0.5 ml HBSS plus 0.26 U/ml Liberase DH [Roche] and 1% sheep serum for 45 minutes at 37\u00b0C while agitating at 750 rpm on an Eppendorf ThermoMixer. Supernatants were collected every 15 min and neutralized with 10% sheep serum  and cells were completely disaggregated by gently pipetting up and down. The dissociated cells were centrifuged at 200 g for 5 min at 4\u00b0C and re suspended in HBSS with 0.05% BSA. The cell suspension 2 ml was then gently added on top of 2 ml HBSS buffer with 7.5% BSA. post spinning down at 200 g for 5 min at 4\u00b0C  the pellet was resuspended in 500 \uf06dl and filtered through a 35 mm strainer.  Whole kidney marrow cells were isolated as described previously. Briefly  kidneys were collected on ice and homogenized immediately in PBS plus 0.05% BSA by using a 10ml syringe with 18 1/2 G. The dissociated cells were washed twice in PBS plus 0.05% BSA and spun down at 300 g for 5 min at 4\u00b0C. Pellets were re suspended in PBS plus 0.05% BSA and filtered through a 35 mm strainer. Livers were collected on ice and incubated in digestion buffer 0.5 ml HBSS plus 0.13 U/ml Liberase DH [Roche] and 1% sheep serum at room temperature. The cell lyses was gently stirred with a Spinbar magnetic stirring bar Bel Art Products  and the supernatant were collected every 5 minutes. The cell lysate was filtered through a 70 mm strainer and centrifuged at 150 g for 3 min at 4\u00b0C. Pellet was resuspended in PBS plus 0.05% BSA and then gently added on top of HBSS buffer with 7.5% BSA. post spinning down at 300 g for 5 min  pellets were washed one more time with PBS plus 0.05% BSA. post centrifuging at 300 g for 5 min  the resuspended cell lyses was filtered through a 35 mm strainer. Pancreatic cells were isolated using the same protocol as liver cells except for the initial spinning down at 300 g for 5 min. All suspended cells were stained with trypan blue and counted using a hemocytometer. Standard library construction protocol using 10x Genomics Chromium Single Cell 3\u2019 GEM  Library & Gel Bead Kit v3  and Chromium Single Cell B Chip Kit.", "Standard zebrafish husbandry protocol.", "tissue:Liver|cell type:whole organ|genotype:wild type|treatment:Heart cryoinjury", "GSM7092946", "GSM7092946: liver 7 dpi replicate 1; Danio rerio; RNA Seq", "GSM7092946 r1", "GSM7092946", "1", "Heart ventricles were placed in the ice cold PBS buffer with 1% BSA. post several washes removing blood cells  ventricles were gently cut into small pieces. Tissues were incubated in digestion buffer 0.5 ml HBSS plus 0.26 U/ml Liberase DH [Roche] and 1% sheep serum for 45 minutes at 37\u00b0C while agitating at 750 rpm on an Eppendorf ThermoMixer. Supernatants were collected every 15 min and neutralized with 10% sheep serum  and cells were completely disaggregated by gently pipetting up and down. The dissociated cells were centrifuged at 200 g for 5 min at 4\u00b0C and re suspended in HBSS with 0.05% BSA. The cell suspension 2 ml was then gently added on top of 2 ml HBSS buffer with 7.5% BSA. post spinning down at 200 g for 5 min at 4\u00b0C  the pellet was resuspended in 500 \uf06dl and filtered through a 35 mm strainer.  Whole kidney marrow cells were isolated as described previously. Briefly  kidneys were collected on ice and homogenized immediately in PBS plus 0.05% BSA by using a 10ml syringe with 18 1/2 G. The dissociated cells were washed twice in PBS plus 0.05% BSA and spun down at 300 g for 5 min at 4\u00b0C. Pellets were re suspended in PBS plus 0.05% BSA and filtered through a 35 mm strainer. Livers were collected on ice and incubated in digestion buffer 0.5 ml HBSS plus 0.13 U/ml Liberase DH [Roche] and 1% sheep serum at room temperature. The cell lyses was gently stirred with a Spinbar magnetic stirring bar Bel Art Products  and the supernatant were collected every 5 minutes. The cell lysate was filtered through a 70 mm strainer and centrifuged at 150 g for 3 min at 4\u00b0C. Pellet was resuspended in PBS plus 0.05% BSA and then gently added on top of HBSS buffer with 7.5% BSA. post spinning down at 300 g for 5 min  pellets were washed one more time with PBS plus 0.05% BSA. post centrifuging at 300 g for 5 min  the resuspended cell lyses was filtered through a 35 mm strainer. Pancreatic cells were isolated using the same protocol as liver cells except for the initial spinning down at 300 g for 5 min. All suspended cells were stained with trypan blue and counted using a hemocytometer. Standard library construction protocol using 10x Genomics Chromium Single Cell three prime GEM  Library & Gel Bead Kit v3  and Chromium Single Cell B Chip Kit.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP426909", null, "loader:fastq load.py", "7dpi-Liver-1_S19_L002_R1_001.fastq.gz 7dpi-Liver-1_S19_L002_R2_001.fastq.gz", "fastq fastq", 3339415222.0, 28300129.0, "GSM7092946 r2", "0:28 1:90", "A:928571814;C:786954831;G:797381996;T:826433990;N:72591", 28, 90, null, null, 928571814, 786954831, 797381996, 826433990, 72591, "SRX19646182", "SRS17013992", "SRA1603354", "Applied Bioinformatics Core, Applied Bioinformatics Core, Weill Cornell Medicine", "Applied Bioinformatics Core, Applied Bioinformatics Core, Weill Cornell Medicine", 2, 0.01263, 0.94485, 0.0034, 0.06446, 0.9935, 0.88341, 0.4136, 0.56226, 28, 90, "T", "B", "sc-like readlen", "illumina", "novaseq_era", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "United States", "2023-03-12", "Undetermined", "Undetermined", "Liver", "Liver and Biliary System"], [74518, "SRR23824362", "SRX19646182", "SRS17013992", "SRP426909", "PRJNA943553", "Single cell transcriptomic analyses of the dynamic local and systemic response to cardiac injury in mice and zebrafish [zebrafish]", "GSE227190", "Transcriptome Analysis", "Mice respond to myocardial infarction MI by generating a fibrotic scar in the heart. This is accompanied by systemic inflammation post the MI resolves. In contrast  zebrafish can fully regenerate their heart post heart injury. To further investigate the divergent species' responses to cardiac injury  single cell transcriptomic analyses were performed on the heart  blood  liver  kidney  and pancreatic islets. Overall design: Upon heart cryoinjury  cells from multiple organs heart  liver  pancreas  and whole kidney marrow were isolated for scRNA seq at 1 and 7 xxx post injury together with the uninjured controls.", "parent bioproject:PRJNA943547", "pubmed:39627536", null, "liver 7 dpi replicate 1", "GSM7092946", null, "source name:Liver|tissue:Liver|cell type:whole organ|genotype:wild type|treatment:Heart cryoinjury", "liver 7 dpi replicate 1", "Demultiplexing  alignment  filtering  and UMI counting was performed with Cell Ranger v6.0.0 using the cellranger count command. Sample integration batch correction  expression value normalization  clustering  cell type annotation and so on were done scater and scran. For more details and the cell type annotation information  see https://github.com/abcwcm/CZI MI mouseZebrafish Assembly: GRCz11 Supplementary files format and content: CellRanger produced filtered feature barcode matrix in H5 format.", "Liver", "Heart cryoinjury or untreated", "Heart ventricles were placed in the ice cold PBS buffer with 1% BSA. post several washes removing blood cells  ventricles were gently cut into small pieces. Tissues were incubated in digestion buffer 0.5 ml HBSS plus 0.26 U/ml Liberase DH [Roche] and 1% sheep serum for 45 minutes at 37\u00b0C while agitating at 750 rpm on an Eppendorf ThermoMixer. Supernatants were collected every 15 min and neutralized with 10% sheep serum  and cells were completely disaggregated by gently pipetting up and down. The dissociated cells were centrifuged at 200 g for 5 min at 4\u00b0C and re suspended in HBSS with 0.05% BSA. The cell suspension 2 ml was then gently added on top of 2 ml HBSS buffer with 7.5% BSA. post spinning down at 200 g for 5 min at 4\u00b0C  the pellet was resuspended in 500 \uf06dl and filtered through a 35 mm strainer.  Whole kidney marrow cells were isolated as described previously. Briefly  kidneys were collected on ice and homogenized immediately in PBS plus 0.05% BSA by using a 10ml syringe with 18 1/2 G. The dissociated cells were washed twice in PBS plus 0.05% BSA and spun down at 300 g for 5 min at 4\u00b0C. Pellets were re suspended in PBS plus 0.05% BSA and filtered through a 35 mm strainer. Livers were collected on ice and incubated in digestion buffer 0.5 ml HBSS plus 0.13 U/ml Liberase DH [Roche] and 1% sheep serum at room temperature. The cell lyses was gently stirred with a Spinbar magnetic stirring bar Bel Art Products  and the supernatant were collected every 5 minutes. The cell lysate was filtered through a 70 mm strainer and centrifuged at 150 g for 3 min at 4\u00b0C. Pellet was resuspended in PBS plus 0.05% BSA and then gently added on top of HBSS buffer with 7.5% BSA. post spinning down at 300 g for 5 min  pellets were washed one more time with PBS plus 0.05% BSA. post centrifuging at 300 g for 5 min  the resuspended cell lyses was filtered through a 35 mm strainer. Pancreatic cells were isolated using the same protocol as liver cells except for the initial spinning down at 300 g for 5 min. All suspended cells were stained with trypan blue and counted using a hemocytometer. Standard library construction protocol using 10x Genomics Chromium Single Cell 3\u2019 GEM  Library & Gel Bead Kit v3  and Chromium Single Cell B Chip Kit.", "Standard zebrafish husbandry protocol.", "tissue:Liver|cell type:whole organ|genotype:wild type|treatment:Heart cryoinjury", "GSM7092946", "GSM7092946: liver 7 dpi replicate 1; Danio rerio; RNA Seq", "GSM7092946 r1", "GSM7092946", "1", "Heart ventricles were placed in the ice cold PBS buffer with 1% BSA. post several washes removing blood cells  ventricles were gently cut into small pieces. Tissues were incubated in digestion buffer 0.5 ml HBSS plus 0.26 U/ml Liberase DH [Roche] and 1% sheep serum for 45 minutes at 37\u00b0C while agitating at 750 rpm on an Eppendorf ThermoMixer. Supernatants were collected every 15 min and neutralized with 10% sheep serum  and cells were completely disaggregated by gently pipetting up and down. The dissociated cells were centrifuged at 200 g for 5 min at 4\u00b0C and re suspended in HBSS with 0.05% BSA. The cell suspension 2 ml was then gently added on top of 2 ml HBSS buffer with 7.5% BSA. post spinning down at 200 g for 5 min at 4\u00b0C  the pellet was resuspended in 500 \uf06dl and filtered through a 35 mm strainer.  Whole kidney marrow cells were isolated as described previously. Briefly  kidneys were collected on ice and homogenized immediately in PBS plus 0.05% BSA by using a 10ml syringe with 18 1/2 G. The dissociated cells were washed twice in PBS plus 0.05% BSA and spun down at 300 g for 5 min at 4\u00b0C. Pellets were re suspended in PBS plus 0.05% BSA and filtered through a 35 mm strainer. Livers were collected on ice and incubated in digestion buffer 0.5 ml HBSS plus 0.13 U/ml Liberase DH [Roche] and 1% sheep serum at room temperature. The cell lyses was gently stirred with a Spinbar magnetic stirring bar Bel Art Products  and the supernatant were collected every 5 minutes. The cell lysate was filtered through a 70 mm strainer and centrifuged at 150 g for 3 min at 4\u00b0C. Pellet was resuspended in PBS plus 0.05% BSA and then gently added on top of HBSS buffer with 7.5% BSA. post spinning down at 300 g for 5 min  pellets were washed one more time with PBS plus 0.05% BSA. post centrifuging at 300 g for 5 min  the resuspended cell lyses was filtered through a 35 mm strainer. Pancreatic cells were isolated using the same protocol as liver cells except for the initial spinning down at 300 g for 5 min. All suspended cells were stained with trypan blue and counted using a hemocytometer. Standard library construction protocol using 10x Genomics Chromium Single Cell three prime GEM  Library & Gel Bead Kit v3  and Chromium Single Cell B Chip Kit.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP426909", null, "loader:fastq load.py", "7dpi-Liver-1_S19_L003_R1_001.fastq.gz 7dpi-Liver-1_S19_L003_R2_001.fastq.gz", "fastq fastq", 3389089446.0, 28721097.0, "GSM7092946 r3", "0:28 1:90", "A:942057789;C:799054157;G:809649273;T:838259397;N:68830", 28, 90, null, null, 942057789, 799054157, 809649273, 838259397, 68830, "SRX19646182", "SRS17013992", "SRA1603354", "Applied Bioinformatics Core, Applied Bioinformatics Core, Weill Cornell Medicine", "Applied Bioinformatics Core, Applied Bioinformatics Core, Weill Cornell Medicine", 2, 0.01251, 0.94572, 0.00331, 0.06443, 0.99279, 0.88469, 0.38873, 0.57466, 28, 90, "T", "B", "sc-like readlen", "illumina", "novaseq_era", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "United States", "2023-03-12", "Undetermined", "Undetermined", "Liver", "Liver and Biliary System"], [74519, "SRR23824363", "SRX19646182", "SRS17013992", "SRP426909", "PRJNA943553", "Single cell transcriptomic analyses of the dynamic local and systemic response to cardiac injury in mice and zebrafish [zebrafish]", "GSE227190", "Transcriptome Analysis", "Mice respond to myocardial infarction MI by generating a fibrotic scar in the heart. This is accompanied by systemic inflammation post the MI resolves. In contrast  zebrafish can fully regenerate their heart post heart injury. To further investigate the divergent species' responses to cardiac injury  single cell transcriptomic analyses were performed on the heart  blood  liver  kidney  and pancreatic islets. Overall design: Upon heart cryoinjury  cells from multiple organs heart  liver  pancreas  and whole kidney marrow were isolated for scRNA seq at 1 and 7 xxx post injury together with the uninjured controls.", "parent bioproject:PRJNA943547", "pubmed:39627536", null, "liver 7 dpi replicate 1", "GSM7092946", null, "source name:Liver|tissue:Liver|cell type:whole organ|genotype:wild type|treatment:Heart cryoinjury", "liver 7 dpi replicate 1", "Demultiplexing  alignment  filtering  and UMI counting was performed with Cell Ranger v6.0.0 using the cellranger count command. Sample integration batch correction  expression value normalization  clustering  cell type annotation and so on were done scater and scran. For more details and the cell type annotation information  see https://github.com/abcwcm/CZI MI mouseZebrafish Assembly: GRCz11 Supplementary files format and content: CellRanger produced filtered feature barcode matrix in H5 format.", "Liver", "Heart cryoinjury or untreated", "Heart ventricles were placed in the ice cold PBS buffer with 1% BSA. post several washes removing blood cells  ventricles were gently cut into small pieces. Tissues were incubated in digestion buffer 0.5 ml HBSS plus 0.26 U/ml Liberase DH [Roche] and 1% sheep serum for 45 minutes at 37\u00b0C while agitating at 750 rpm on an Eppendorf ThermoMixer. Supernatants were collected every 15 min and neutralized with 10% sheep serum  and cells were completely disaggregated by gently pipetting up and down. The dissociated cells were centrifuged at 200 g for 5 min at 4\u00b0C and re suspended in HBSS with 0.05% BSA. The cell suspension 2 ml was then gently added on top of 2 ml HBSS buffer with 7.5% BSA. post spinning down at 200 g for 5 min at 4\u00b0C  the pellet was resuspended in 500 \uf06dl and filtered through a 35 mm strainer.  Whole kidney marrow cells were isolated as described previously. Briefly  kidneys were collected on ice and homogenized immediately in PBS plus 0.05% BSA by using a 10ml syringe with 18 1/2 G. The dissociated cells were washed twice in PBS plus 0.05% BSA and spun down at 300 g for 5 min at 4\u00b0C. Pellets were re suspended in PBS plus 0.05% BSA and filtered through a 35 mm strainer. Livers were collected on ice and incubated in digestion buffer 0.5 ml HBSS plus 0.13 U/ml Liberase DH [Roche] and 1% sheep serum at room temperature. The cell lyses was gently stirred with a Spinbar magnetic stirring bar Bel Art Products  and the supernatant were collected every 5 minutes. The cell lysate was filtered through a 70 mm strainer and centrifuged at 150 g for 3 min at 4\u00b0C. Pellet was resuspended in PBS plus 0.05% BSA and then gently added on top of HBSS buffer with 7.5% BSA. post spinning down at 300 g for 5 min  pellets were washed one more time with PBS plus 0.05% BSA. post centrifuging at 300 g for 5 min  the resuspended cell lyses was filtered through a 35 mm strainer. Pancreatic cells were isolated using the same protocol as liver cells except for the initial spinning down at 300 g for 5 min. All suspended cells were stained with trypan blue and counted using a hemocytometer. Standard library construction protocol using 10x Genomics Chromium Single Cell 3\u2019 GEM  Library & Gel Bead Kit v3  and Chromium Single Cell B Chip Kit.", "Standard zebrafish husbandry protocol.", "tissue:Liver|cell type:whole organ|genotype:wild type|treatment:Heart cryoinjury", "GSM7092946", "GSM7092946: liver 7 dpi replicate 1; Danio rerio; RNA Seq", "GSM7092946 r1", "GSM7092946", "1", "Heart ventricles were placed in the ice cold PBS buffer with 1% BSA. post several washes removing blood cells  ventricles were gently cut into small pieces. Tissues were incubated in digestion buffer 0.5 ml HBSS plus 0.26 U/ml Liberase DH [Roche] and 1% sheep serum for 45 minutes at 37\u00b0C while agitating at 750 rpm on an Eppendorf ThermoMixer. Supernatants were collected every 15 min and neutralized with 10% sheep serum  and cells were completely disaggregated by gently pipetting up and down. The dissociated cells were centrifuged at 200 g for 5 min at 4\u00b0C and re suspended in HBSS with 0.05% BSA. The cell suspension 2 ml was then gently added on top of 2 ml HBSS buffer with 7.5% BSA. post spinning down at 200 g for 5 min at 4\u00b0C  the pellet was resuspended in 500 \uf06dl and filtered through a 35 mm strainer.  Whole kidney marrow cells were isolated as described previously. Briefly  kidneys were collected on ice and homogenized immediately in PBS plus 0.05% BSA by using a 10ml syringe with 18 1/2 G. The dissociated cells were washed twice in PBS plus 0.05% BSA and spun down at 300 g for 5 min at 4\u00b0C. Pellets were re suspended in PBS plus 0.05% BSA and filtered through a 35 mm strainer. Livers were collected on ice and incubated in digestion buffer 0.5 ml HBSS plus 0.13 U/ml Liberase DH [Roche] and 1% sheep serum at room temperature. The cell lyses was gently stirred with a Spinbar magnetic stirring bar Bel Art Products  and the supernatant were collected every 5 minutes. The cell lysate was filtered through a 70 mm strainer and centrifuged at 150 g for 3 min at 4\u00b0C. Pellet was resuspended in PBS plus 0.05% BSA and then gently added on top of HBSS buffer with 7.5% BSA. post spinning down at 300 g for 5 min  pellets were washed one more time with PBS plus 0.05% BSA. post centrifuging at 300 g for 5 min  the resuspended cell lyses was filtered through a 35 mm strainer. Pancreatic cells were isolated using the same protocol as liver cells except for the initial spinning down at 300 g for 5 min. All suspended cells were stained with trypan blue and counted using a hemocytometer. Standard library construction protocol using 10x Genomics Chromium Single Cell three prime GEM  Library & Gel Bead Kit v3  and Chromium Single Cell B Chip Kit.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP426909", null, "loader:fastq load.py", "7dpi-Liver-1_S19_L004_R1_001.fastq.gz 7dpi-Liver-1_S19_L004_R2_001.fastq.gz", "fastq fastq", 3326239460.0, 28188470.0, "GSM7092946 r4", "0:28 1:90", "A:925663531;C:783373500;G:793504177;T:823634415;N:63837", 28, 90, null, null, 925663531, 783373500, 793504177, 823634415, 63837, "SRX19646182", "SRS17013992", "SRA1603354", "Applied Bioinformatics Core, Applied Bioinformatics Core, Weill Cornell Medicine", "Applied Bioinformatics Core, Applied Bioinformatics Core, Weill Cornell Medicine", 2, 0.01246, 0.94489, 0.00337, 0.06529, 0.9933, 0.88556, 0.39241, 0.55747, 28, 90, "T", "B", "sc-like readlen", "illumina", "novaseq_era", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "United States", "2023-03-12", "Undetermined", "Undetermined", "Liver", "Liver and Biliary System"], [74540, "SRR23824378", "SRX19646176", "SRS17013986", "SRP426909", "PRJNA943553", "Single cell transcriptomic analyses of the dynamic local and systemic response to cardiac injury in mice and zebrafish [zebrafish]", "GSE227190", "Transcriptome Analysis", "Mice respond to myocardial infarction MI by generating a fibrotic scar in the heart. This is accompanied by systemic inflammation post the MI resolves. In contrast  zebrafish can fully regenerate their heart post heart injury. To further investigate the divergent species' responses to cardiac injury  single cell transcriptomic analyses were performed on the heart  blood  liver  kidney  and pancreatic islets. Overall design: Upon heart cryoinjury  cells from multiple organs heart  liver  pancreas  and whole kidney marrow were isolated for scRNA seq at 1 and 7 xxx post injury together with the uninjured controls.", "parent bioproject:PRJNA943547", "pubmed:39627536", null, "liver 1 dpi replicate 2", "GSM7092939", null, "source name:Liver|tissue:Liver|cell type:whole organ|genotype:wild type|treatment:Heart cryoinjury", "liver 1 dpi replicate 2", "Demultiplexing  alignment  filtering  and UMI counting was performed with Cell Ranger v6.0.0 using the cellranger count command. Sample integration batch correction  expression value normalization  clustering  cell type annotation and so on were done scater and scran. For more details and the cell type annotation information  see https://github.com/abcwcm/CZI MI mouseZebrafish Assembly: GRCz11 Supplementary files format and content: CellRanger produced filtered feature barcode matrix in H5 format.", "Liver", "Heart cryoinjury or untreated", "Heart ventricles were placed in the ice cold PBS buffer with 1% BSA. post several washes removing blood cells  ventricles were gently cut into small pieces. Tissues were incubated in digestion buffer 0.5 ml HBSS plus 0.26 U/ml Liberase DH [Roche] and 1% sheep serum for 45 minutes at 37\u00b0C while agitating at 750 rpm on an Eppendorf ThermoMixer. Supernatants were collected every 15 min and neutralized with 10% sheep serum  and cells were completely disaggregated by gently pipetting up and down. The dissociated cells were centrifuged at 200 g for 5 min at 4\u00b0C and re suspended in HBSS with 0.05% BSA. The cell suspension 2 ml was then gently added on top of 2 ml HBSS buffer with 7.5% BSA. post spinning down at 200 g for 5 min at 4\u00b0C  the pellet was resuspended in 500 \uf06dl and filtered through a 35 mm strainer.  Whole kidney marrow cells were isolated as described previously. Briefly  kidneys were collected on ice and homogenized immediately in PBS plus 0.05% BSA by using a 10ml syringe with 18 1/2 G. The dissociated cells were washed twice in PBS plus 0.05% BSA and spun down at 300 g for 5 min at 4\u00b0C. Pellets were re suspended in PBS plus 0.05% BSA and filtered through a 35 mm strainer. Livers were collected on ice and incubated in digestion buffer 0.5 ml HBSS plus 0.13 U/ml Liberase DH [Roche] and 1% sheep serum at room temperature. The cell lyses was gently stirred with a Spinbar magnetic stirring bar Bel Art Products  and the supernatant were collected every 5 minutes. The cell lysate was filtered through a 70 mm strainer and centrifuged at 150 g for 3 min at 4\u00b0C. Pellet was resuspended in PBS plus 0.05% BSA and then gently added on top of HBSS buffer with 7.5% BSA. post spinning down at 300 g for 5 min  pellets were washed one more time with PBS plus 0.05% BSA. post centrifuging at 300 g for 5 min  the resuspended cell lyses was filtered through a 35 mm strainer. Pancreatic cells were isolated using the same protocol as liver cells except for the initial spinning down at 300 g for 5 min. All suspended cells were stained with trypan blue and counted using a hemocytometer. Standard library construction protocol using 10x Genomics Chromium Single Cell 3\u2019 GEM  Library & Gel Bead Kit v3  and Chromium Single Cell B Chip Kit.", "Standard zebrafish husbandry protocol.", "tissue:Liver|cell type:whole organ|genotype:wild type|treatment:Heart cryoinjury", "GSM7092939", "GSM7092939: liver 1 dpi replicate 2; Danio rerio; RNA Seq", "GSM7092939 r1", "GSM7092939", "1", "Heart ventricles were placed in the ice cold PBS buffer with 1% BSA. post several washes removing blood cells  ventricles were gently cut into small pieces. Tissues were incubated in digestion buffer 0.5 ml HBSS plus 0.26 U/ml Liberase DH [Roche] and 1% sheep serum for 45 minutes at 37\u00b0C while agitating at 750 rpm on an Eppendorf ThermoMixer. Supernatants were collected every 15 min and neutralized with 10% sheep serum  and cells were completely disaggregated by gently pipetting up and down. The dissociated cells were centrifuged at 200 g for 5 min at 4\u00b0C and re suspended in HBSS with 0.05% BSA. The cell suspension 2 ml was then gently added on top of 2 ml HBSS buffer with 7.5% BSA. post spinning down at 200 g for 5 min at 4\u00b0C  the pellet was resuspended in 500 \uf06dl and filtered through a 35 mm strainer.  Whole kidney marrow cells were isolated as described previously. Briefly  kidneys were collected on ice and homogenized immediately in PBS plus 0.05% BSA by using a 10ml syringe with 18 1/2 G. The dissociated cells were washed twice in PBS plus 0.05% BSA and spun down at 300 g for 5 min at 4\u00b0C. Pellets were re suspended in PBS plus 0.05% BSA and filtered through a 35 mm strainer. Livers were collected on ice and incubated in digestion buffer 0.5 ml HBSS plus 0.13 U/ml Liberase DH [Roche] and 1% sheep serum at room temperature. The cell lyses was gently stirred with a Spinbar magnetic stirring bar Bel Art Products  and the supernatant were collected every 5 minutes. The cell lysate was filtered through a 70 mm strainer and centrifuged at 150 g for 3 min at 4\u00b0C. Pellet was resuspended in PBS plus 0.05% BSA and then gently added on top of HBSS buffer with 7.5% BSA. post spinning down at 300 g for 5 min  pellets were washed one more time with PBS plus 0.05% BSA. post centrifuging at 300 g for 5 min  the resuspended cell lyses was filtered through a 35 mm strainer. Pancreatic cells were isolated using the same protocol as liver cells except for the initial spinning down at 300 g for 5 min. All suspended cells were stained with trypan blue and counted using a hemocytometer. Standard library construction protocol using 10x Genomics Chromium Single Cell three prime GEM  Library & Gel Bead Kit v3  and Chromium Single Cell B Chip Kit.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP426909", null, "loader:fastq load.py", "1dpi-Liver-2_S15_L001_R1_001.fastq.gz 1dpi-Liver-2_S15_L001_R2_001.fastq.gz", "fastq fastq", 3974430452.0, 33681614.0, "GSM7092939 r1", "0:28 1:90", "A:1095528573;C:930228144;G:956778552;T:991804101;N:91082", 28, 90, null, null, 1095528573, 930228144, 956778552, 991804101, 91082, "SRX19646176", "SRS17013986", "SRA1603354", "Applied Bioinformatics Core, Applied Bioinformatics Core, Weill Cornell Medicine", "Applied Bioinformatics Core, Applied Bioinformatics Core, Weill Cornell Medicine", 2, 0.0077, 0.93007, 0.00172, 0.08628, 0.99308, 0.86448, 0.28435, 0.51435, 28, 90, "T", "B", "sc-like readlen", "illumina", "novaseq_era", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "United States", "2023-03-12", "Undetermined", "Undetermined", "Liver", "Liver and Biliary System"], [74541, "SRR23824379", "SRX19646176", "SRS17013986", "SRP426909", "PRJNA943553", "Single cell transcriptomic analyses of the dynamic local and systemic response to cardiac injury in mice and zebrafish [zebrafish]", "GSE227190", "Transcriptome Analysis", "Mice respond to myocardial infarction MI by generating a fibrotic scar in the heart. This is accompanied by systemic inflammation post the MI resolves. In contrast  zebrafish can fully regenerate their heart post heart injury. To further investigate the divergent species' responses to cardiac injury  single cell transcriptomic analyses were performed on the heart  blood  liver  kidney  and pancreatic islets. Overall design: Upon heart cryoinjury  cells from multiple organs heart  liver  pancreas  and whole kidney marrow were isolated for scRNA seq at 1 and 7 xxx post injury together with the uninjured controls.", "parent bioproject:PRJNA943547", "pubmed:39627536", null, "liver 1 dpi replicate 2", "GSM7092939", null, "source name:Liver|tissue:Liver|cell type:whole organ|genotype:wild type|treatment:Heart cryoinjury", "liver 1 dpi replicate 2", "Demultiplexing  alignment  filtering  and UMI counting was performed with Cell Ranger v6.0.0 using the cellranger count command. Sample integration batch correction  expression value normalization  clustering  cell type annotation and so on were done scater and scran. For more details and the cell type annotation information  see https://github.com/abcwcm/CZI MI mouseZebrafish Assembly: GRCz11 Supplementary files format and content: CellRanger produced filtered feature barcode matrix in H5 format.", "Liver", "Heart cryoinjury or untreated", "Heart ventricles were placed in the ice cold PBS buffer with 1% BSA. post several washes removing blood cells  ventricles were gently cut into small pieces. Tissues were incubated in digestion buffer 0.5 ml HBSS plus 0.26 U/ml Liberase DH [Roche] and 1% sheep serum for 45 minutes at 37\u00b0C while agitating at 750 rpm on an Eppendorf ThermoMixer. Supernatants were collected every 15 min and neutralized with 10% sheep serum  and cells were completely disaggregated by gently pipetting up and down. The dissociated cells were centrifuged at 200 g for 5 min at 4\u00b0C and re suspended in HBSS with 0.05% BSA. The cell suspension 2 ml was then gently added on top of 2 ml HBSS buffer with 7.5% BSA. post spinning down at 200 g for 5 min at 4\u00b0C  the pellet was resuspended in 500 \uf06dl and filtered through a 35 mm strainer.  Whole kidney marrow cells were isolated as described previously. Briefly  kidneys were collected on ice and homogenized immediately in PBS plus 0.05% BSA by using a 10ml syringe with 18 1/2 G. The dissociated cells were washed twice in PBS plus 0.05% BSA and spun down at 300 g for 5 min at 4\u00b0C. Pellets were re suspended in PBS plus 0.05% BSA and filtered through a 35 mm strainer. Livers were collected on ice and incubated in digestion buffer 0.5 ml HBSS plus 0.13 U/ml Liberase DH [Roche] and 1% sheep serum at room temperature. The cell lyses was gently stirred with a Spinbar magnetic stirring bar Bel Art Products  and the supernatant were collected every 5 minutes. The cell lysate was filtered through a 70 mm strainer and centrifuged at 150 g for 3 min at 4\u00b0C. Pellet was resuspended in PBS plus 0.05% BSA and then gently added on top of HBSS buffer with 7.5% BSA. post spinning down at 300 g for 5 min  pellets were washed one more time with PBS plus 0.05% BSA. post centrifuging at 300 g for 5 min  the resuspended cell lyses was filtered through a 35 mm strainer. Pancreatic cells were isolated using the same protocol as liver cells except for the initial spinning down at 300 g for 5 min. All suspended cells were stained with trypan blue and counted using a hemocytometer. Standard library construction protocol using 10x Genomics Chromium Single Cell 3\u2019 GEM  Library & Gel Bead Kit v3  and Chromium Single Cell B Chip Kit.", "Standard zebrafish husbandry protocol.", "tissue:Liver|cell type:whole organ|genotype:wild type|treatment:Heart cryoinjury", "GSM7092939", "GSM7092939: liver 1 dpi replicate 2; Danio rerio; RNA Seq", "GSM7092939 r1", "GSM7092939", "1", "Heart ventricles were placed in the ice cold PBS buffer with 1% BSA. post several washes removing blood cells  ventricles were gently cut into small pieces. Tissues were incubated in digestion buffer 0.5 ml HBSS plus 0.26 U/ml Liberase DH [Roche] and 1% sheep serum for 45 minutes at 37\u00b0C while agitating at 750 rpm on an Eppendorf ThermoMixer. Supernatants were collected every 15 min and neutralized with 10% sheep serum  and cells were completely disaggregated by gently pipetting up and down. The dissociated cells were centrifuged at 200 g for 5 min at 4\u00b0C and re suspended in HBSS with 0.05% BSA. The cell suspension 2 ml was then gently added on top of 2 ml HBSS buffer with 7.5% BSA. post spinning down at 200 g for 5 min at 4\u00b0C  the pellet was resuspended in 500 \uf06dl and filtered through a 35 mm strainer.  Whole kidney marrow cells were isolated as described previously. Briefly  kidneys were collected on ice and homogenized immediately in PBS plus 0.05% BSA by using a 10ml syringe with 18 1/2 G. The dissociated cells were washed twice in PBS plus 0.05% BSA and spun down at 300 g for 5 min at 4\u00b0C. Pellets were re suspended in PBS plus 0.05% BSA and filtered through a 35 mm strainer. Livers were collected on ice and incubated in digestion buffer 0.5 ml HBSS plus 0.13 U/ml Liberase DH [Roche] and 1% sheep serum at room temperature. The cell lyses was gently stirred with a Spinbar magnetic stirring bar Bel Art Products  and the supernatant were collected every 5 minutes. The cell lysate was filtered through a 70 mm strainer and centrifuged at 150 g for 3 min at 4\u00b0C. Pellet was resuspended in PBS plus 0.05% BSA and then gently added on top of HBSS buffer with 7.5% BSA. post spinning down at 300 g for 5 min  pellets were washed one more time with PBS plus 0.05% BSA. post centrifuging at 300 g for 5 min  the resuspended cell lyses was filtered through a 35 mm strainer. Pancreatic cells were isolated using the same protocol as liver cells except for the initial spinning down at 300 g for 5 min. All suspended cells were stained with trypan blue and counted using a hemocytometer. Standard library construction protocol using 10x Genomics Chromium Single Cell three prime GEM  Library & Gel Bead Kit v3  and Chromium Single Cell B Chip Kit.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP426909", null, "loader:fastq load.py", "1dpi-Liver-2_S15_L002_R1_001.fastq.gz 1dpi-Liver-2_S15_L002_R2_001.fastq.gz", "fastq fastq", 4016739824.0, 34040168.0, "GSM7092939 r2", "0:28 1:90", "A:1107054312;C:940103463;G:966907262;T:1002588445;N:86342", 28, 90, null, null, 1107054312, 940103463, 966907262, 1002588445, 86342, "SRX19646176", "SRS17013986", "SRA1603354", "Applied Bioinformatics Core, Applied Bioinformatics Core, Weill Cornell Medicine", "Applied Bioinformatics Core, Applied Bioinformatics Core, Weill Cornell Medicine", 2, 0.00824, 0.92962, 0.00199, 0.08535, 0.99318, 0.86444, 0.2722, 0.50248, 28, 90, "T", "B", "sc-like readlen", "illumina", "novaseq_era", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "United States", "2023-03-12", "Undetermined", "Undetermined", "Liver", "Liver and Biliary System"], [74542, "SRR23824402", "SRX19646176", "SRS17013986", "SRP426909", "PRJNA943553", "Single cell transcriptomic analyses of the dynamic local and systemic response to cardiac injury in mice and zebrafish [zebrafish]", "GSE227190", "Transcriptome Analysis", "Mice respond to myocardial infarction MI by generating a fibrotic scar in the heart. This is accompanied by systemic inflammation post the MI resolves. In contrast  zebrafish can fully regenerate their heart post heart injury. To further investigate the divergent species' responses to cardiac injury  single cell transcriptomic analyses were performed on the heart  blood  liver  kidney  and pancreatic islets. Overall design: Upon heart cryoinjury  cells from multiple organs heart  liver  pancreas  and whole kidney marrow were isolated for scRNA seq at 1 and 7 xxx post injury together with the uninjured controls.", "parent bioproject:PRJNA943547", "pubmed:39627536", null, "liver 1 dpi replicate 2", "GSM7092939", null, "source name:Liver|tissue:Liver|cell type:whole organ|genotype:wild type|treatment:Heart cryoinjury", "liver 1 dpi replicate 2", "Demultiplexing  alignment  filtering  and UMI counting was performed with Cell Ranger v6.0.0 using the cellranger count command. Sample integration batch correction  expression value normalization  clustering  cell type annotation and so on were done scater and scran. For more details and the cell type annotation information  see https://github.com/abcwcm/CZI MI mouseZebrafish Assembly: GRCz11 Supplementary files format and content: CellRanger produced filtered feature barcode matrix in H5 format.", "Liver", "Heart cryoinjury or untreated", "Heart ventricles were placed in the ice cold PBS buffer with 1% BSA. post several washes removing blood cells  ventricles were gently cut into small pieces. Tissues were incubated in digestion buffer 0.5 ml HBSS plus 0.26 U/ml Liberase DH [Roche] and 1% sheep serum for 45 minutes at 37\u00b0C while agitating at 750 rpm on an Eppendorf ThermoMixer. Supernatants were collected every 15 min and neutralized with 10% sheep serum  and cells were completely disaggregated by gently pipetting up and down. The dissociated cells were centrifuged at 200 g for 5 min at 4\u00b0C and re suspended in HBSS with 0.05% BSA. The cell suspension 2 ml was then gently added on top of 2 ml HBSS buffer with 7.5% BSA. post spinning down at 200 g for 5 min at 4\u00b0C  the pellet was resuspended in 500 \uf06dl and filtered through a 35 mm strainer.  Whole kidney marrow cells were isolated as described previously. Briefly  kidneys were collected on ice and homogenized immediately in PBS plus 0.05% BSA by using a 10ml syringe with 18 1/2 G. The dissociated cells were washed twice in PBS plus 0.05% BSA and spun down at 300 g for 5 min at 4\u00b0C. Pellets were re suspended in PBS plus 0.05% BSA and filtered through a 35 mm strainer. Livers were collected on ice and incubated in digestion buffer 0.5 ml HBSS plus 0.13 U/ml Liberase DH [Roche] and 1% sheep serum at room temperature. The cell lyses was gently stirred with a Spinbar magnetic stirring bar Bel Art Products  and the supernatant were collected every 5 minutes. The cell lysate was filtered through a 70 mm strainer and centrifuged at 150 g for 3 min at 4\u00b0C. Pellet was resuspended in PBS plus 0.05% BSA and then gently added on top of HBSS buffer with 7.5% BSA. post spinning down at 300 g for 5 min  pellets were washed one more time with PBS plus 0.05% BSA. post centrifuging at 300 g for 5 min  the resuspended cell lyses was filtered through a 35 mm strainer. Pancreatic cells were isolated using the same protocol as liver cells except for the initial spinning down at 300 g for 5 min. All suspended cells were stained with trypan blue and counted using a hemocytometer. Standard library construction protocol using 10x Genomics Chromium Single Cell 3\u2019 GEM  Library & Gel Bead Kit v3  and Chromium Single Cell B Chip Kit.", "Standard zebrafish husbandry protocol.", "tissue:Liver|cell type:whole organ|genotype:wild type|treatment:Heart cryoinjury", "GSM7092939", "GSM7092939: liver 1 dpi replicate 2; Danio rerio; RNA Seq", "GSM7092939 r1", "GSM7092939", "1", "Heart ventricles were placed in the ice cold PBS buffer with 1% BSA. post several washes removing blood cells  ventricles were gently cut into small pieces. Tissues were incubated in digestion buffer 0.5 ml HBSS plus 0.26 U/ml Liberase DH [Roche] and 1% sheep serum for 45 minutes at 37\u00b0C while agitating at 750 rpm on an Eppendorf ThermoMixer. Supernatants were collected every 15 min and neutralized with 10% sheep serum  and cells were completely disaggregated by gently pipetting up and down. The dissociated cells were centrifuged at 200 g for 5 min at 4\u00b0C and re suspended in HBSS with 0.05% BSA. The cell suspension 2 ml was then gently added on top of 2 ml HBSS buffer with 7.5% BSA. post spinning down at 200 g for 5 min at 4\u00b0C  the pellet was resuspended in 500 \uf06dl and filtered through a 35 mm strainer.  Whole kidney marrow cells were isolated as described previously. Briefly  kidneys were collected on ice and homogenized immediately in PBS plus 0.05% BSA by using a 10ml syringe with 18 1/2 G. The dissociated cells were washed twice in PBS plus 0.05% BSA and spun down at 300 g for 5 min at 4\u00b0C. Pellets were re suspended in PBS plus 0.05% BSA and filtered through a 35 mm strainer. Livers were collected on ice and incubated in digestion buffer 0.5 ml HBSS plus 0.13 U/ml Liberase DH [Roche] and 1% sheep serum at room temperature. The cell lyses was gently stirred with a Spinbar magnetic stirring bar Bel Art Products  and the supernatant were collected every 5 minutes. The cell lysate was filtered through a 70 mm strainer and centrifuged at 150 g for 3 min at 4\u00b0C. Pellet was resuspended in PBS plus 0.05% BSA and then gently added on top of HBSS buffer with 7.5% BSA. post spinning down at 300 g for 5 min  pellets were washed one more time with PBS plus 0.05% BSA. post centrifuging at 300 g for 5 min  the resuspended cell lyses was filtered through a 35 mm strainer. Pancreatic cells were isolated using the same protocol as liver cells except for the initial spinning down at 300 g for 5 min. All suspended cells were stained with trypan blue and counted using a hemocytometer. Standard library construction protocol using 10x Genomics Chromium Single Cell three prime GEM  Library & Gel Bead Kit v3  and Chromium Single Cell B Chip Kit.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP426909", null, "loader:fastq load.py", "1dpi-Liver-2_S15_L003_R1_001.fastq.gz 1dpi-Liver-2_S15_L003_R2_001.fastq.gz", "fastq fastq", 4043460452.0, 34266614.0, "GSM7092939 r3", "0:28 1:90", "A:1113324543;C:946991884;G:974101052;T:1008961554;N:81419", 28, 90, null, null, 1113324543, 946991884, 974101052, 1008961554, 81419, "SRX19646176", "SRS17013986", "SRA1603354", "Applied Bioinformatics Core, Applied Bioinformatics Core, Weill Cornell Medicine", "Applied Bioinformatics Core, Applied Bioinformatics Core, Weill Cornell Medicine", 2, 0.00748, 0.93039, 0.00169, 0.08606, 0.99289, 0.86716, 0.29448, 0.51523, 28, 90, "T", "B", "sc-like readlen", "illumina", "novaseq_era", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "United States", "2023-03-12", "Undetermined", "Undetermined", "Liver", "Liver and Biliary System"], [74543, "SRR23824403", "SRX19646176", "SRS17013986", "SRP426909", "PRJNA943553", "Single cell transcriptomic analyses of the dynamic local and systemic response to cardiac injury in mice and zebrafish [zebrafish]", "GSE227190", "Transcriptome Analysis", "Mice respond to myocardial infarction MI by generating a fibrotic scar in the heart. This is accompanied by systemic inflammation post the MI resolves. In contrast  zebrafish can fully regenerate their heart post heart injury. To further investigate the divergent species' responses to cardiac injury  single cell transcriptomic analyses were performed on the heart  blood  liver  kidney  and pancreatic islets. Overall design: Upon heart cryoinjury  cells from multiple organs heart  liver  pancreas  and whole kidney marrow were isolated for scRNA seq at 1 and 7 xxx post injury together with the uninjured controls.", "parent bioproject:PRJNA943547", "pubmed:39627536", null, "liver 1 dpi replicate 2", "GSM7092939", null, "source name:Liver|tissue:Liver|cell type:whole organ|genotype:wild type|treatment:Heart cryoinjury", "liver 1 dpi replicate 2", "Demultiplexing  alignment  filtering  and UMI counting was performed with Cell Ranger v6.0.0 using the cellranger count command. Sample integration batch correction  expression value normalization  clustering  cell type annotation and so on were done scater and scran. For more details and the cell type annotation information  see https://github.com/abcwcm/CZI MI mouseZebrafish Assembly: GRCz11 Supplementary files format and content: CellRanger produced filtered feature barcode matrix in H5 format.", "Liver", "Heart cryoinjury or untreated", "Heart ventricles were placed in the ice cold PBS buffer with 1% BSA. post several washes removing blood cells  ventricles were gently cut into small pieces. Tissues were incubated in digestion buffer 0.5 ml HBSS plus 0.26 U/ml Liberase DH [Roche] and 1% sheep serum for 45 minutes at 37\u00b0C while agitating at 750 rpm on an Eppendorf ThermoMixer. Supernatants were collected every 15 min and neutralized with 10% sheep serum  and cells were completely disaggregated by gently pipetting up and down. The dissociated cells were centrifuged at 200 g for 5 min at 4\u00b0C and re suspended in HBSS with 0.05% BSA. The cell suspension 2 ml was then gently added on top of 2 ml HBSS buffer with 7.5% BSA. post spinning down at 200 g for 5 min at 4\u00b0C  the pellet was resuspended in 500 \uf06dl and filtered through a 35 mm strainer.  Whole kidney marrow cells were isolated as described previously. Briefly  kidneys were collected on ice and homogenized immediately in PBS plus 0.05% BSA by using a 10ml syringe with 18 1/2 G. The dissociated cells were washed twice in PBS plus 0.05% BSA and spun down at 300 g for 5 min at 4\u00b0C. Pellets were re suspended in PBS plus 0.05% BSA and filtered through a 35 mm strainer. Livers were collected on ice and incubated in digestion buffer 0.5 ml HBSS plus 0.13 U/ml Liberase DH [Roche] and 1% sheep serum at room temperature. The cell lyses was gently stirred with a Spinbar magnetic stirring bar Bel Art Products  and the supernatant were collected every 5 minutes. The cell lysate was filtered through a 70 mm strainer and centrifuged at 150 g for 3 min at 4\u00b0C. Pellet was resuspended in PBS plus 0.05% BSA and then gently added on top of HBSS buffer with 7.5% BSA. post spinning down at 300 g for 5 min  pellets were washed one more time with PBS plus 0.05% BSA. post centrifuging at 300 g for 5 min  the resuspended cell lyses was filtered through a 35 mm strainer. Pancreatic cells were isolated using the same protocol as liver cells except for the initial spinning down at 300 g for 5 min. All suspended cells were stained with trypan blue and counted using a hemocytometer. Standard library construction protocol using 10x Genomics Chromium Single Cell 3\u2019 GEM  Library & Gel Bead Kit v3  and Chromium Single Cell B Chip Kit.", "Standard zebrafish husbandry protocol.", "tissue:Liver|cell type:whole organ|genotype:wild type|treatment:Heart cryoinjury", "GSM7092939", "GSM7092939: liver 1 dpi replicate 2; Danio rerio; RNA Seq", "GSM7092939 r1", "GSM7092939", "1", "Heart ventricles were placed in the ice cold PBS buffer with 1% BSA. post several washes removing blood cells  ventricles were gently cut into small pieces. Tissues were incubated in digestion buffer 0.5 ml HBSS plus 0.26 U/ml Liberase DH [Roche] and 1% sheep serum for 45 minutes at 37\u00b0C while agitating at 750 rpm on an Eppendorf ThermoMixer. Supernatants were collected every 15 min and neutralized with 10% sheep serum  and cells were completely disaggregated by gently pipetting up and down. The dissociated cells were centrifuged at 200 g for 5 min at 4\u00b0C and re suspended in HBSS with 0.05% BSA. The cell suspension 2 ml was then gently added on top of 2 ml HBSS buffer with 7.5% BSA. post spinning down at 200 g for 5 min at 4\u00b0C  the pellet was resuspended in 500 \uf06dl and filtered through a 35 mm strainer.  Whole kidney marrow cells were isolated as described previously. Briefly  kidneys were collected on ice and homogenized immediately in PBS plus 0.05% BSA by using a 10ml syringe with 18 1/2 G. The dissociated cells were washed twice in PBS plus 0.05% BSA and spun down at 300 g for 5 min at 4\u00b0C. Pellets were re suspended in PBS plus 0.05% BSA and filtered through a 35 mm strainer. Livers were collected on ice and incubated in digestion buffer 0.5 ml HBSS plus 0.13 U/ml Liberase DH [Roche] and 1% sheep serum at room temperature. The cell lyses was gently stirred with a Spinbar magnetic stirring bar Bel Art Products  and the supernatant were collected every 5 minutes. The cell lysate was filtered through a 70 mm strainer and centrifuged at 150 g for 3 min at 4\u00b0C. Pellet was resuspended in PBS plus 0.05% BSA and then gently added on top of HBSS buffer with 7.5% BSA. post spinning down at 300 g for 5 min  pellets were washed one more time with PBS plus 0.05% BSA. post centrifuging at 300 g for 5 min  the resuspended cell lyses was filtered through a 35 mm strainer. Pancreatic cells were isolated using the same protocol as liver cells except for the initial spinning down at 300 g for 5 min. All suspended cells were stained with trypan blue and counted using a hemocytometer. Standard library construction protocol using 10x Genomics Chromium Single Cell three prime GEM  Library & Gel Bead Kit v3  and Chromium Single Cell B Chip Kit.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP426909", null, "loader:fastq load.py", "1dpi-Liver-2_S15_L004_R1_001.fastq.gz 1dpi-Liver-2_S15_L004_R2_001.fastq.gz", "fastq fastq", 3977097134.0, 33704213.0, "GSM7092939 r4", "0:28 1:90", "A:1096872592;C:930325395;G:956420114;T:993402280;N:76753", 28, 90, null, null, 1096872592, 930325395, 956420114, 993402280, 76753, "SRX19646176", "SRS17013986", "SRA1603354", "Applied Bioinformatics Core, Applied Bioinformatics Core, Weill Cornell Medicine", "Applied Bioinformatics Core, Applied Bioinformatics Core, Weill Cornell Medicine", 2, 0.00756, 0.93049, 0.00182, 0.08655, 0.99354, 0.86488, 0.29659, 0.50942, 28, 90, "T", "B", "sc-like readlen", "illumina", "novaseq_era", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "United States", "2023-03-12", "Undetermined", "Undetermined", "Liver", "Liver and Biliary System"], [74552, "SRR23824392", "SRX19646173", "SRS17013982", "SRP426909", "PRJNA943553", "Single cell transcriptomic analyses of the dynamic local and systemic response to cardiac injury in mice and zebrafish [zebrafish]", "GSE227190", "Transcriptome Analysis", "Mice respond to myocardial infarction MI by generating a fibrotic scar in the heart. This is accompanied by systemic inflammation post the MI resolves. In contrast  zebrafish can fully regenerate their heart post heart injury. To further investigate the divergent species' responses to cardiac injury  single cell transcriptomic analyses were performed on the heart  blood  liver  kidney  and pancreatic islets. Overall design: Upon heart cryoinjury  cells from multiple organs heart  liver  pancreas  and whole kidney marrow were isolated for scRNA seq at 1 and 7 xxx post injury together with the uninjured controls.", "parent bioproject:PRJNA943547", "pubmed:39627536", null, "liver 1 dpi replicate 1", "GSM7092938", null, "source name:Liver|tissue:Liver|cell type:whole organ|genotype:wild type|treatment:Heart cryoinjury", "liver 1 dpi replicate 1", "Demultiplexing  alignment  filtering  and UMI counting was performed with Cell Ranger v6.0.0 using the cellranger count command. Sample integration batch correction  expression value normalization  clustering  cell type annotation and so on were done scater and scran. For more details and the cell type annotation information  see https://github.com/abcwcm/CZI MI mouseZebrafish Assembly: GRCz11 Supplementary files format and content: CellRanger produced filtered feature barcode matrix in H5 format.", "Liver", "Heart cryoinjury or untreated", "Heart ventricles were placed in the ice cold PBS buffer with 1% BSA. post several washes removing blood cells  ventricles were gently cut into small pieces. Tissues were incubated in digestion buffer 0.5 ml HBSS plus 0.26 U/ml Liberase DH [Roche] and 1% sheep serum for 45 minutes at 37\u00b0C while agitating at 750 rpm on an Eppendorf ThermoMixer. Supernatants were collected every 15 min and neutralized with 10% sheep serum  and cells were completely disaggregated by gently pipetting up and down. The dissociated cells were centrifuged at 200 g for 5 min at 4\u00b0C and re suspended in HBSS with 0.05% BSA. The cell suspension 2 ml was then gently added on top of 2 ml HBSS buffer with 7.5% BSA. post spinning down at 200 g for 5 min at 4\u00b0C  the pellet was resuspended in 500 \uf06dl and filtered through a 35 mm strainer.  Whole kidney marrow cells were isolated as described previously. Briefly  kidneys were collected on ice and homogenized immediately in PBS plus 0.05% BSA by using a 10ml syringe with 18 1/2 G. The dissociated cells were washed twice in PBS plus 0.05% BSA and spun down at 300 g for 5 min at 4\u00b0C. Pellets were re suspended in PBS plus 0.05% BSA and filtered through a 35 mm strainer. Livers were collected on ice and incubated in digestion buffer 0.5 ml HBSS plus 0.13 U/ml Liberase DH [Roche] and 1% sheep serum at room temperature. The cell lyses was gently stirred with a Spinbar magnetic stirring bar Bel Art Products  and the supernatant were collected every 5 minutes. The cell lysate was filtered through a 70 mm strainer and centrifuged at 150 g for 3 min at 4\u00b0C. Pellet was resuspended in PBS plus 0.05% BSA and then gently added on top of HBSS buffer with 7.5% BSA. post spinning down at 300 g for 5 min  pellets were washed one more time with PBS plus 0.05% BSA. post centrifuging at 300 g for 5 min  the resuspended cell lyses was filtered through a 35 mm strainer. Pancreatic cells were isolated using the same protocol as liver cells except for the initial spinning down at 300 g for 5 min. All suspended cells were stained with trypan blue and counted using a hemocytometer. Standard library construction protocol using 10x Genomics Chromium Single Cell 3\u2019 GEM  Library & Gel Bead Kit v3  and Chromium Single Cell B Chip Kit.", "Standard zebrafish husbandry protocol.", "tissue:Liver|cell type:whole organ|genotype:wild type|treatment:Heart cryoinjury", "GSM7092938", "GSM7092938: liver 1 dpi replicate 1; Danio rerio; RNA Seq", "GSM7092938 r1", "GSM7092938", "1", "Heart ventricles were placed in the ice cold PBS buffer with 1% BSA. post several washes removing blood cells  ventricles were gently cut into small pieces. Tissues were incubated in digestion buffer 0.5 ml HBSS plus 0.26 U/ml Liberase DH [Roche] and 1% sheep serum for 45 minutes at 37\u00b0C while agitating at 750 rpm on an Eppendorf ThermoMixer. Supernatants were collected every 15 min and neutralized with 10% sheep serum  and cells were completely disaggregated by gently pipetting up and down. The dissociated cells were centrifuged at 200 g for 5 min at 4\u00b0C and re suspended in HBSS with 0.05% BSA. The cell suspension 2 ml was then gently added on top of 2 ml HBSS buffer with 7.5% BSA. post spinning down at 200 g for 5 min at 4\u00b0C  the pellet was resuspended in 500 \uf06dl and filtered through a 35 mm strainer.  Whole kidney marrow cells were isolated as described previously. Briefly  kidneys were collected on ice and homogenized immediately in PBS plus 0.05% BSA by using a 10ml syringe with 18 1/2 G. The dissociated cells were washed twice in PBS plus 0.05% BSA and spun down at 300 g for 5 min at 4\u00b0C. Pellets were re suspended in PBS plus 0.05% BSA and filtered through a 35 mm strainer. Livers were collected on ice and incubated in digestion buffer 0.5 ml HBSS plus 0.13 U/ml Liberase DH [Roche] and 1% sheep serum at room temperature. The cell lyses was gently stirred with a Spinbar magnetic stirring bar Bel Art Products  and the supernatant were collected every 5 minutes. The cell lysate was filtered through a 70 mm strainer and centrifuged at 150 g for 3 min at 4\u00b0C. Pellet was resuspended in PBS plus 0.05% BSA and then gently added on top of HBSS buffer with 7.5% BSA. post spinning down at 300 g for 5 min  pellets were washed one more time with PBS plus 0.05% BSA. post centrifuging at 300 g for 5 min  the resuspended cell lyses was filtered through a 35 mm strainer. Pancreatic cells were isolated using the same protocol as liver cells except for the initial spinning down at 300 g for 5 min. All suspended cells were stained with trypan blue and counted using a hemocytometer. Standard library construction protocol using 10x Genomics Chromium Single Cell three prime GEM  Library & Gel Bead Kit v3  and Chromium Single Cell B Chip Kit.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP426909", null, "loader:fastq load.py", "1dpi-Liver-1_S11_L001_R1_001.fastq.gz 1dpi-Liver-1_S11_L001_R2_001.fastq.gz", "fastq fastq", 4161718400.0, 35268800.0, "GSM7092938 r1", "0:28 1:90", "A:1156237978;C:969526154;G:993733251;T:1042125334;N:95683", 28, 90, null, null, 1156237978, 969526154, 993733251, 1042125334, 95683, "SRX19646173", "SRS17013982", "SRA1603354", "Applied Bioinformatics Core, Applied Bioinformatics Core, Weill Cornell Medicine", "Applied Bioinformatics Core, Applied Bioinformatics Core, Weill Cornell Medicine", 2, 0.00742, 0.93962, 0.00193, 0.08534, 0.99405, 0.86931, 0.28289, 0.47838, 28, 90, "T", "B", "sc-like readlen", "illumina", "novaseq_era", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "United States", "2023-03-12", "Undetermined", "Undetermined", "Liver", "Liver and Biliary System"], [74553, "SRR23824393", "SRX19646173", "SRS17013982", "SRP426909", "PRJNA943553", "Single cell transcriptomic analyses of the dynamic local and systemic response to cardiac injury in mice and zebrafish [zebrafish]", "GSE227190", "Transcriptome Analysis", "Mice respond to myocardial infarction MI by generating a fibrotic scar in the heart. This is accompanied by systemic inflammation post the MI resolves. In contrast  zebrafish can fully regenerate their heart post heart injury. To further investigate the divergent species' responses to cardiac injury  single cell transcriptomic analyses were performed on the heart  blood  liver  kidney  and pancreatic islets. Overall design: Upon heart cryoinjury  cells from multiple organs heart  liver  pancreas  and whole kidney marrow were isolated for scRNA seq at 1 and 7 xxx post injury together with the uninjured controls.", "parent bioproject:PRJNA943547", "pubmed:39627536", null, "liver 1 dpi replicate 1", "GSM7092938", null, "source name:Liver|tissue:Liver|cell type:whole organ|genotype:wild type|treatment:Heart cryoinjury", "liver 1 dpi replicate 1", "Demultiplexing  alignment  filtering  and UMI counting was performed with Cell Ranger v6.0.0 using the cellranger count command. Sample integration batch correction  expression value normalization  clustering  cell type annotation and so on were done scater and scran. For more details and the cell type annotation information  see https://github.com/abcwcm/CZI MI mouseZebrafish Assembly: GRCz11 Supplementary files format and content: CellRanger produced filtered feature barcode matrix in H5 format.", "Liver", "Heart cryoinjury or untreated", "Heart ventricles were placed in the ice cold PBS buffer with 1% BSA. post several washes removing blood cells  ventricles were gently cut into small pieces. Tissues were incubated in digestion buffer 0.5 ml HBSS plus 0.26 U/ml Liberase DH [Roche] and 1% sheep serum for 45 minutes at 37\u00b0C while agitating at 750 rpm on an Eppendorf ThermoMixer. Supernatants were collected every 15 min and neutralized with 10% sheep serum  and cells were completely disaggregated by gently pipetting up and down. The dissociated cells were centrifuged at 200 g for 5 min at 4\u00b0C and re suspended in HBSS with 0.05% BSA. The cell suspension 2 ml was then gently added on top of 2 ml HBSS buffer with 7.5% BSA. post spinning down at 200 g for 5 min at 4\u00b0C  the pellet was resuspended in 500 \uf06dl and filtered through a 35 mm strainer.  Whole kidney marrow cells were isolated as described previously. Briefly  kidneys were collected on ice and homogenized immediately in PBS plus 0.05% BSA by using a 10ml syringe with 18 1/2 G. The dissociated cells were washed twice in PBS plus 0.05% BSA and spun down at 300 g for 5 min at 4\u00b0C. Pellets were re suspended in PBS plus 0.05% BSA and filtered through a 35 mm strainer. Livers were collected on ice and incubated in digestion buffer 0.5 ml HBSS plus 0.13 U/ml Liberase DH [Roche] and 1% sheep serum at room temperature. The cell lyses was gently stirred with a Spinbar magnetic stirring bar Bel Art Products  and the supernatant were collected every 5 minutes. The cell lysate was filtered through a 70 mm strainer and centrifuged at 150 g for 3 min at 4\u00b0C. Pellet was resuspended in PBS plus 0.05% BSA and then gently added on top of HBSS buffer with 7.5% BSA. post spinning down at 300 g for 5 min  pellets were washed one more time with PBS plus 0.05% BSA. post centrifuging at 300 g for 5 min  the resuspended cell lyses was filtered through a 35 mm strainer. Pancreatic cells were isolated using the same protocol as liver cells except for the initial spinning down at 300 g for 5 min. All suspended cells were stained with trypan blue and counted using a hemocytometer. Standard library construction protocol using 10x Genomics Chromium Single Cell 3\u2019 GEM  Library & Gel Bead Kit v3  and Chromium Single Cell B Chip Kit.", "Standard zebrafish husbandry protocol.", "tissue:Liver|cell type:whole organ|genotype:wild type|treatment:Heart cryoinjury", "GSM7092938", "GSM7092938: liver 1 dpi replicate 1; Danio rerio; RNA Seq", "GSM7092938 r1", "GSM7092938", "1", "Heart ventricles were placed in the ice cold PBS buffer with 1% BSA. post several washes removing blood cells  ventricles were gently cut into small pieces. Tissues were incubated in digestion buffer 0.5 ml HBSS plus 0.26 U/ml Liberase DH [Roche] and 1% sheep serum for 45 minutes at 37\u00b0C while agitating at 750 rpm on an Eppendorf ThermoMixer. Supernatants were collected every 15 min and neutralized with 10% sheep serum  and cells were completely disaggregated by gently pipetting up and down. The dissociated cells were centrifuged at 200 g for 5 min at 4\u00b0C and re suspended in HBSS with 0.05% BSA. The cell suspension 2 ml was then gently added on top of 2 ml HBSS buffer with 7.5% BSA. post spinning down at 200 g for 5 min at 4\u00b0C  the pellet was resuspended in 500 \uf06dl and filtered through a 35 mm strainer.  Whole kidney marrow cells were isolated as described previously. Briefly  kidneys were collected on ice and homogenized immediately in PBS plus 0.05% BSA by using a 10ml syringe with 18 1/2 G. The dissociated cells were washed twice in PBS plus 0.05% BSA and spun down at 300 g for 5 min at 4\u00b0C. Pellets were re suspended in PBS plus 0.05% BSA and filtered through a 35 mm strainer. Livers were collected on ice and incubated in digestion buffer 0.5 ml HBSS plus 0.13 U/ml Liberase DH [Roche] and 1% sheep serum at room temperature. The cell lyses was gently stirred with a Spinbar magnetic stirring bar Bel Art Products  and the supernatant were collected every 5 minutes. The cell lysate was filtered through a 70 mm strainer and centrifuged at 150 g for 3 min at 4\u00b0C. Pellet was resuspended in PBS plus 0.05% BSA and then gently added on top of HBSS buffer with 7.5% BSA. post spinning down at 300 g for 5 min  pellets were washed one more time with PBS plus 0.05% BSA. post centrifuging at 300 g for 5 min  the resuspended cell lyses was filtered through a 35 mm strainer. Pancreatic cells were isolated using the same protocol as liver cells except for the initial spinning down at 300 g for 5 min. All suspended cells were stained with trypan blue and counted using a hemocytometer. Standard library construction protocol using 10x Genomics Chromium Single Cell three prime GEM  Library & Gel Bead Kit v3  and Chromium Single Cell B Chip Kit.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP426909", null, "loader:fastq load.py", "1dpi-Liver-1_S11_L002_R1_001.fastq.gz 1dpi-Liver-1_S11_L002_R2_001.fastq.gz", "fastq fastq", 4194311652.0, 35545014.0, "GSM7092938 r2", "0:28 1:90", "A:1164793350;C:977205946;G:1001366769;T:1050855725;N:89862", 28, 90, null, null, 1164793350, 977205946, 1001366769, 1050855725, 89862, "SRX19646173", "SRS17013982", "SRA1603354", "Applied Bioinformatics Core, Applied Bioinformatics Core, Weill Cornell Medicine", "Applied Bioinformatics Core, Applied Bioinformatics Core, Weill Cornell Medicine", 2, 0.0076, 0.9407, 0.0018, 0.08601, 0.99389, 0.86831, 0.256, 0.50037, 28, 90, "T", "B", "sc-like readlen", "illumina", "novaseq_era", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "United States", "2023-03-12", "Undetermined", "Undetermined", "Liver", "Liver and Biliary System"], [74554, "SRR23824400", "SRX19646173", "SRS17013982", "SRP426909", "PRJNA943553", "Single cell transcriptomic analyses of the dynamic local and systemic response to cardiac injury in mice and zebrafish [zebrafish]", "GSE227190", "Transcriptome Analysis", "Mice respond to myocardial infarction MI by generating a fibrotic scar in the heart. This is accompanied by systemic inflammation post the MI resolves. In contrast  zebrafish can fully regenerate their heart post heart injury. To further investigate the divergent species' responses to cardiac injury  single cell transcriptomic analyses were performed on the heart  blood  liver  kidney  and pancreatic islets. Overall design: Upon heart cryoinjury  cells from multiple organs heart  liver  pancreas  and whole kidney marrow were isolated for scRNA seq at 1 and 7 xxx post injury together with the uninjured controls.", "parent bioproject:PRJNA943547", "pubmed:39627536", null, "liver 1 dpi replicate 1", "GSM7092938", null, "source name:Liver|tissue:Liver|cell type:whole organ|genotype:wild type|treatment:Heart cryoinjury", "liver 1 dpi replicate 1", "Demultiplexing  alignment  filtering  and UMI counting was performed with Cell Ranger v6.0.0 using the cellranger count command. Sample integration batch correction  expression value normalization  clustering  cell type annotation and so on were done scater and scran. For more details and the cell type annotation information  see https://github.com/abcwcm/CZI MI mouseZebrafish Assembly: GRCz11 Supplementary files format and content: CellRanger produced filtered feature barcode matrix in H5 format.", "Liver", "Heart cryoinjury or untreated", "Heart ventricles were placed in the ice cold PBS buffer with 1% BSA. post several washes removing blood cells  ventricles were gently cut into small pieces. Tissues were incubated in digestion buffer 0.5 ml HBSS plus 0.26 U/ml Liberase DH [Roche] and 1% sheep serum for 45 minutes at 37\u00b0C while agitating at 750 rpm on an Eppendorf ThermoMixer. Supernatants were collected every 15 min and neutralized with 10% sheep serum  and cells were completely disaggregated by gently pipetting up and down. The dissociated cells were centrifuged at 200 g for 5 min at 4\u00b0C and re suspended in HBSS with 0.05% BSA. The cell suspension 2 ml was then gently added on top of 2 ml HBSS buffer with 7.5% BSA. post spinning down at 200 g for 5 min at 4\u00b0C  the pellet was resuspended in 500 \uf06dl and filtered through a 35 mm strainer.  Whole kidney marrow cells were isolated as described previously. Briefly  kidneys were collected on ice and homogenized immediately in PBS plus 0.05% BSA by using a 10ml syringe with 18 1/2 G. The dissociated cells were washed twice in PBS plus 0.05% BSA and spun down at 300 g for 5 min at 4\u00b0C. Pellets were re suspended in PBS plus 0.05% BSA and filtered through a 35 mm strainer. Livers were collected on ice and incubated in digestion buffer 0.5 ml HBSS plus 0.13 U/ml Liberase DH [Roche] and 1% sheep serum at room temperature. The cell lyses was gently stirred with a Spinbar magnetic stirring bar Bel Art Products  and the supernatant were collected every 5 minutes. The cell lysate was filtered through a 70 mm strainer and centrifuged at 150 g for 3 min at 4\u00b0C. Pellet was resuspended in PBS plus 0.05% BSA and then gently added on top of HBSS buffer with 7.5% BSA. post spinning down at 300 g for 5 min  pellets were washed one more time with PBS plus 0.05% BSA. post centrifuging at 300 g for 5 min  the resuspended cell lyses was filtered through a 35 mm strainer. Pancreatic cells were isolated using the same protocol as liver cells except for the initial spinning down at 300 g for 5 min. All suspended cells were stained with trypan blue and counted using a hemocytometer. Standard library construction protocol using 10x Genomics Chromium Single Cell 3\u2019 GEM  Library & Gel Bead Kit v3  and Chromium Single Cell B Chip Kit.", "Standard zebrafish husbandry protocol.", "tissue:Liver|cell type:whole organ|genotype:wild type|treatment:Heart cryoinjury", "GSM7092938", "GSM7092938: liver 1 dpi replicate 1; Danio rerio; RNA Seq", "GSM7092938 r1", "GSM7092938", "1", "Heart ventricles were placed in the ice cold PBS buffer with 1% BSA. post several washes removing blood cells  ventricles were gently cut into small pieces. Tissues were incubated in digestion buffer 0.5 ml HBSS plus 0.26 U/ml Liberase DH [Roche] and 1% sheep serum for 45 minutes at 37\u00b0C while agitating at 750 rpm on an Eppendorf ThermoMixer. Supernatants were collected every 15 min and neutralized with 10% sheep serum  and cells were completely disaggregated by gently pipetting up and down. The dissociated cells were centrifuged at 200 g for 5 min at 4\u00b0C and re suspended in HBSS with 0.05% BSA. The cell suspension 2 ml was then gently added on top of 2 ml HBSS buffer with 7.5% BSA. post spinning down at 200 g for 5 min at 4\u00b0C  the pellet was resuspended in 500 \uf06dl and filtered through a 35 mm strainer.  Whole kidney marrow cells were isolated as described previously. Briefly  kidneys were collected on ice and homogenized immediately in PBS plus 0.05% BSA by using a 10ml syringe with 18 1/2 G. The dissociated cells were washed twice in PBS plus 0.05% BSA and spun down at 300 g for 5 min at 4\u00b0C. Pellets were re suspended in PBS plus 0.05% BSA and filtered through a 35 mm strainer. Livers were collected on ice and incubated in digestion buffer 0.5 ml HBSS plus 0.13 U/ml Liberase DH [Roche] and 1% sheep serum at room temperature. The cell lyses was gently stirred with a Spinbar magnetic stirring bar Bel Art Products  and the supernatant were collected every 5 minutes. The cell lysate was filtered through a 70 mm strainer and centrifuged at 150 g for 3 min at 4\u00b0C. Pellet was resuspended in PBS plus 0.05% BSA and then gently added on top of HBSS buffer with 7.5% BSA. post spinning down at 300 g for 5 min  pellets were washed one more time with PBS plus 0.05% BSA. post centrifuging at 300 g for 5 min  the resuspended cell lyses was filtered through a 35 mm strainer. Pancreatic cells were isolated using the same protocol as liver cells except for the initial spinning down at 300 g for 5 min. All suspended cells were stained with trypan blue and counted using a hemocytometer. Standard library construction protocol using 10x Genomics Chromium Single Cell three prime GEM  Library & Gel Bead Kit v3  and Chromium Single Cell B Chip Kit.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP426909", null, "loader:fastq load.py", "1dpi-Liver-1_S11_L003_R1_001.fastq.gz 1dpi-Liver-1_S11_L003_R2_001.fastq.gz", "fastq fastq", 4236970186.0, 35906527.0, "GSM7092938 r3", "0:28 1:90", "A:1175860194;C:987702516;G:1012476545;T:1060846608;N:84323", 28, 90, null, null, 1175860194, 987702516, 1012476545, 1060846608, 84323, "SRX19646173", "SRS17013982", "SRA1603354", "Applied Bioinformatics Core, Applied Bioinformatics Core, Weill Cornell Medicine", "Applied Bioinformatics Core, Applied Bioinformatics Core, Weill Cornell Medicine", 2, 0.00758, 0.94031, 0.00187, 0.08613, 0.99381, 0.86819, 0.28494, 0.50439, 28, 90, "T", "B", "sc-like readlen", "illumina", "novaseq_era", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "United States", "2023-03-12", "Undetermined", "Undetermined", "Liver", "Liver and Biliary System"], [74555, "SRR23824401", "SRX19646173", "SRS17013982", "SRP426909", "PRJNA943553", "Single cell transcriptomic analyses of the dynamic local and systemic response to cardiac injury in mice and zebrafish [zebrafish]", "GSE227190", "Transcriptome Analysis", "Mice respond to myocardial infarction MI by generating a fibrotic scar in the heart. This is accompanied by systemic inflammation post the MI resolves. In contrast  zebrafish can fully regenerate their heart post heart injury. To further investigate the divergent species' responses to cardiac injury  single cell transcriptomic analyses were performed on the heart  blood  liver  kidney  and pancreatic islets. Overall design: Upon heart cryoinjury  cells from multiple organs heart  liver  pancreas  and whole kidney marrow were isolated for scRNA seq at 1 and 7 xxx post injury together with the uninjured controls.", "parent bioproject:PRJNA943547", "pubmed:39627536", null, "liver 1 dpi replicate 1", "GSM7092938", null, "source name:Liver|tissue:Liver|cell type:whole organ|genotype:wild type|treatment:Heart cryoinjury", "liver 1 dpi replicate 1", "Demultiplexing  alignment  filtering  and UMI counting was performed with Cell Ranger v6.0.0 using the cellranger count command. Sample integration batch correction  expression value normalization  clustering  cell type annotation and so on were done scater and scran. For more details and the cell type annotation information  see https://github.com/abcwcm/CZI MI mouseZebrafish Assembly: GRCz11 Supplementary files format and content: CellRanger produced filtered feature barcode matrix in H5 format.", "Liver", "Heart cryoinjury or untreated", "Heart ventricles were placed in the ice cold PBS buffer with 1% BSA. post several washes removing blood cells  ventricles were gently cut into small pieces. Tissues were incubated in digestion buffer 0.5 ml HBSS plus 0.26 U/ml Liberase DH [Roche] and 1% sheep serum for 45 minutes at 37\u00b0C while agitating at 750 rpm on an Eppendorf ThermoMixer. Supernatants were collected every 15 min and neutralized with 10% sheep serum  and cells were completely disaggregated by gently pipetting up and down. The dissociated cells were centrifuged at 200 g for 5 min at 4\u00b0C and re suspended in HBSS with 0.05% BSA. The cell suspension 2 ml was then gently added on top of 2 ml HBSS buffer with 7.5% BSA. post spinning down at 200 g for 5 min at 4\u00b0C  the pellet was resuspended in 500 \uf06dl and filtered through a 35 mm strainer.  Whole kidney marrow cells were isolated as described previously. Briefly  kidneys were collected on ice and homogenized immediately in PBS plus 0.05% BSA by using a 10ml syringe with 18 1/2 G. The dissociated cells were washed twice in PBS plus 0.05% BSA and spun down at 300 g for 5 min at 4\u00b0C. Pellets were re suspended in PBS plus 0.05% BSA and filtered through a 35 mm strainer. Livers were collected on ice and incubated in digestion buffer 0.5 ml HBSS plus 0.13 U/ml Liberase DH [Roche] and 1% sheep serum at room temperature. The cell lyses was gently stirred with a Spinbar magnetic stirring bar Bel Art Products  and the supernatant were collected every 5 minutes. The cell lysate was filtered through a 70 mm strainer and centrifuged at 150 g for 3 min at 4\u00b0C. Pellet was resuspended in PBS plus 0.05% BSA and then gently added on top of HBSS buffer with 7.5% BSA. post spinning down at 300 g for 5 min  pellets were washed one more time with PBS plus 0.05% BSA. post centrifuging at 300 g for 5 min  the resuspended cell lyses was filtered through a 35 mm strainer. Pancreatic cells were isolated using the same protocol as liver cells except for the initial spinning down at 300 g for 5 min. All suspended cells were stained with trypan blue and counted using a hemocytometer. Standard library construction protocol using 10x Genomics Chromium Single Cell 3\u2019 GEM  Library & Gel Bead Kit v3  and Chromium Single Cell B Chip Kit.", "Standard zebrafish husbandry protocol.", "tissue:Liver|cell type:whole organ|genotype:wild type|treatment:Heart cryoinjury", "GSM7092938", "GSM7092938: liver 1 dpi replicate 1; Danio rerio; RNA Seq", "GSM7092938 r1", "GSM7092938", "1", "Heart ventricles were placed in the ice cold PBS buffer with 1% BSA. post several washes removing blood cells  ventricles were gently cut into small pieces. Tissues were incubated in digestion buffer 0.5 ml HBSS plus 0.26 U/ml Liberase DH [Roche] and 1% sheep serum for 45 minutes at 37\u00b0C while agitating at 750 rpm on an Eppendorf ThermoMixer. Supernatants were collected every 15 min and neutralized with 10% sheep serum  and cells were completely disaggregated by gently pipetting up and down. The dissociated cells were centrifuged at 200 g for 5 min at 4\u00b0C and re suspended in HBSS with 0.05% BSA. The cell suspension 2 ml was then gently added on top of 2 ml HBSS buffer with 7.5% BSA. post spinning down at 200 g for 5 min at 4\u00b0C  the pellet was resuspended in 500 \uf06dl and filtered through a 35 mm strainer.  Whole kidney marrow cells were isolated as described previously. Briefly  kidneys were collected on ice and homogenized immediately in PBS plus 0.05% BSA by using a 10ml syringe with 18 1/2 G. The dissociated cells were washed twice in PBS plus 0.05% BSA and spun down at 300 g for 5 min at 4\u00b0C. Pellets were re suspended in PBS plus 0.05% BSA and filtered through a 35 mm strainer. Livers were collected on ice and incubated in digestion buffer 0.5 ml HBSS plus 0.13 U/ml Liberase DH [Roche] and 1% sheep serum at room temperature. The cell lyses was gently stirred with a Spinbar magnetic stirring bar Bel Art Products  and the supernatant were collected every 5 minutes. The cell lysate was filtered through a 70 mm strainer and centrifuged at 150 g for 3 min at 4\u00b0C. Pellet was resuspended in PBS plus 0.05% BSA and then gently added on top of HBSS buffer with 7.5% BSA. post spinning down at 300 g for 5 min  pellets were washed one more time with PBS plus 0.05% BSA. post centrifuging at 300 g for 5 min  the resuspended cell lyses was filtered through a 35 mm strainer. Pancreatic cells were isolated using the same protocol as liver cells except for the initial spinning down at 300 g for 5 min. All suspended cells were stained with trypan blue and counted using a hemocytometer. Standard library construction protocol using 10x Genomics Chromium Single Cell three prime GEM  Library & Gel Bead Kit v3  and Chromium Single Cell B Chip Kit.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP426909", null, "loader:fastq load.py", "1dpi-Liver-1_S11_L004_R1_001.fastq.gz 1dpi-Liver-1_S11_L004_R2_001.fastq.gz", "fastq fastq", 4158915900.0, 35245050.0, "GSM7092938 r4", "0:28 1:90", "A:1156072890;C:968215633;G:991941070;T:1042607465;N:78842", 28, 90, null, null, 1156072890, 968215633, 991941070, 1042607465, 78842, "SRX19646173", "SRS17013982", "SRA1603354", "Applied Bioinformatics Core, Applied Bioinformatics Core, Weill Cornell Medicine", "Applied Bioinformatics Core, Applied Bioinformatics Core, Weill Cornell Medicine", 2, 0.00789, 0.9395, 0.00206, 0.08611, 0.99352, 0.86963, 0.28343, 0.50985, 28, 90, "T", "B", "sc-like readlen", "illumina", "novaseq_era", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "United States", "2023-03-12", "Undetermined", "Undetermined", "Liver", "Liver and Biliary System"]], "truncated": false, "filtered_table_rows_count": 106, "expanded_columns": [], "expandable_columns": [], "columns": ["rowid", "run.accession", "experiment.accession", "sample.accession", "study.accession", "bioproject", "study.title", "study.alias", "study.type", "study.abstract", "study.attributes", "study.PMIDs", "sample.description", "sample.title", "sample.alias", "sample.centername", "sample.attributes", "GEOsample.title", "GEOsample.dataprocessing", "GEOsample.source", "GEOsample.treatmentprotocol", "GEOsample.extractprotocol", "GEOsample.growthprotocol", "GEOsample.characteristics", "GEOsample.accession", "experiment.title", "experiment.alias", "experiment.library_name", "experiment.design_description", "experiment.library_construction_protocol", "experiment.attributes", "experiment.library_strategy", "experiment.library_source", "experiment.library_selection", "experiment.library_layout", "experiment.platform", "experiment.instrument_model", "experiment.spot_descriptor", "experiment.study_ref", "run.title", "run.attributes", "run.filename", "run.semantic_name", "run.total_bases", "run.total_spots", "run.alias", "run.read_lengths", "run.base_counts", "run.r1_length", "run.r2_length", "run.r3_length", "run.r4_length", "run.Acount", "run.Ccount", "run.Gcount", "run.Tcount", "run.Ncount", "run.experiment", "run.pool_member", "submission.accession", "submission.srasource", "submission.bioprojectsource", "seqdetective.n_mates", "seqdetective.mapping_rate.mate1", "seqdetective.mapping_rate.mate2", "seqdetective.nofeature_rate.mate1", "seqdetective.nofeature_rate.mate2", "seqdetective.sparsity.mate1", "seqdetective.sparsity.mate2", "seqdetective.pos_strand_rate.mate1", "seqdetective.pos_strand_rate.mate2", "seqdetective.readlen.mate1", "seqdetective.readlen.mate2", "seqdetective.judgement.mate1", "seqdetective.judgement.mate2", "seqdetective.judgement.reason", "platform_family", "instrument_generation", "read_bias", "selection_class", "prep_kit", "sc_or_bulk", "tech_class", "technology", "tech_variant", "submission.bioprojectsource.country", "earliest_date", "devstage_curation", "devstage_curation_coarse", "tissue_curation", "tissue_curation_coarse"], "primary_keys": [], "units": {}, "query": {"sql": "select rowid, [run.accession], [experiment.accession], [sample.accession], [study.accession], bioproject, [study.title], [study.alias], [study.type], [study.abstract], [study.attributes], [study.PMIDs], [sample.description], [sample.title], [sample.alias], [sample.centername], [sample.attributes], [GEOsample.title], [GEOsample.dataprocessing], [GEOsample.source], [GEOsample.treatmentprotocol], [GEOsample.extractprotocol], [GEOsample.growthprotocol], [GEOsample.characteristics], [GEOsample.accession], [experiment.title], [experiment.alias], [experiment.library_name], [experiment.design_description], [experiment.library_construction_protocol], [experiment.attributes], [experiment.library_strategy], [experiment.library_source], [experiment.library_selection], [experiment.library_layout], [experiment.platform], [experiment.instrument_model], [experiment.spot_descriptor], [experiment.study_ref], [run.title], [run.attributes], [run.filename], [run.semantic_name], [run.total_bases], [run.total_spots], [run.alias], [run.read_lengths], [run.base_counts], [run.r1_length], [run.r2_length], [run.r3_length], [run.r4_length], [run.Acount], [run.Ccount], [run.Gcount], [run.Tcount], [run.Ncount], [run.experiment], [run.pool_member], [submission.accession], [submission.srasource], [submission.bioprojectsource], [seqdetective.n_mates], [seqdetective.mapping_rate.mate1], [seqdetective.mapping_rate.mate2], [seqdetective.nofeature_rate.mate1], [seqdetective.nofeature_rate.mate2], [seqdetective.sparsity.mate1], [seqdetective.sparsity.mate2], [seqdetective.pos_strand_rate.mate1], [seqdetective.pos_strand_rate.mate2], [seqdetective.readlen.mate1], [seqdetective.readlen.mate2], [seqdetective.judgement.mate1], [seqdetective.judgement.mate2], [seqdetective.judgement.reason], platform_family, instrument_generation, read_bias, selection_class, prep_kit, sc_or_bulk, tech_class, technology, tech_variant, [submission.bioprojectsource.country], earliest_date, devstage_curation, devstage_curation_coarse, tissue_curation, tissue_curation_coarse from run_metadata where \"experiment.library_source\" = :p0 and \"tissue_curation_coarse\" = :p1 order by rowid limit 101", "params": {"p0": "TRANSCRIPTOMIC SINGLE CELL", "p1": "Liver and Biliary System"}}, "facet_results": {"experiment.library_strategy": {"name": "experiment.library_strategy", "type": "column", "hideable": false, "toggle_url": "/metadata/run_metadata.json?experiment.library_source=TRANSCRIPTOMIC+SINGLE+CELL&tissue_curation_coarse=Liver+and+Biliary+System", "results": [{"value": "RNA-Seq", "label": "RNA-Seq", "count": 106, "toggle_url": "http://metadata.rnaquarium.org/metadata/run_metadata.json?experiment.library_source=TRANSCRIPTOMIC+SINGLE+CELL&tissue_curation_coarse=Liver+and+Biliary+System&experiment.library_strategy=RNA-Seq", "selected": false}], "truncated": false}, "experiment.library_source": {"name": "experiment.library_source", "type": "column", "hideable": false, "toggle_url": "/metadata/run_metadata.json?experiment.library_source=TRANSCRIPTOMIC+SINGLE+CELL&tissue_curation_coarse=Liver+and+Biliary+System", "results": [{"value": "TRANSCRIPTOMIC SINGLE CELL", "label": "TRANSCRIPTOMIC SINGLE CELL", "count": 106, "toggle_url": "http://metadata.rnaquarium.org/metadata/run_metadata.json?tissue_curation_coarse=Liver+and+Biliary+System", "selected": true}], "truncated": false}, "experiment.library_selection": {"name": "experiment.library_selection", "type": "column", "hideable": false, "toggle_url": "/metadata/run_metadata.json?experiment.library_source=TRANSCRIPTOMIC+SINGLE+CELL&tissue_curation_coarse=Liver+and+Biliary+System", "results": [{"value": "cDNA", "label": "cDNA", "count": 100, "toggle_url": "http://metadata.rnaquarium.org/metadata/run_metadata.json?experiment.library_source=TRANSCRIPTOMIC+SINGLE+CELL&tissue_curation_coarse=Liver+and+Biliary+System&experiment.library_selection=cDNA", "selected": false}, {"value": "Oligo-dT", "label": "Oligo-dT", "count": 6, "toggle_url": "http://metadata.rnaquarium.org/metadata/run_metadata.json?experiment.library_source=TRANSCRIPTOMIC+SINGLE+CELL&tissue_curation_coarse=Liver+and+Biliary+System&experiment.library_selection=Oligo-dT", "selected": false}], "truncated": false}, "experiment.library_layout": {"name": "experiment.library_layout", "type": "column", "hideable": false, "toggle_url": "/metadata/run_metadata.json?experiment.library_source=TRANSCRIPTOMIC+SINGLE+CELL&tissue_curation_coarse=Liver+and+Biliary+System", "results": [{"value": "PAIRED", "label": "PAIRED", "count": 106, "toggle_url": "http://metadata.rnaquarium.org/metadata/run_metadata.json?experiment.library_source=TRANSCRIPTOMIC+SINGLE+CELL&tissue_curation_coarse=Liver+and+Biliary+System&experiment.library_layout=PAIRED", "selected": false}], "truncated": false}, "experiment.platform": {"name": "experiment.platform", "type": "column", "hideable": false, "toggle_url": "/metadata/run_metadata.json?experiment.library_source=TRANSCRIPTOMIC+SINGLE+CELL&tissue_curation_coarse=Liver+and+Biliary+System", "results": [{"value": "ILLUMINA", "label": "ILLUMINA", "count": 106, "toggle_url": "http://metadata.rnaquarium.org/metadata/run_metadata.json?experiment.library_source=TRANSCRIPTOMIC+SINGLE+CELL&tissue_curation_coarse=Liver+and+Biliary+System&experiment.platform=ILLUMINA", "selected": false}], "truncated": false}, "devstage_curation_coarse": {"name": "devstage_curation_coarse", "type": "column", "hideable": false, "toggle_url": "/metadata/run_metadata.json?experiment.library_source=TRANSCRIPTOMIC+SINGLE+CELL&tissue_curation_coarse=Liver+and+Biliary+System", "results": [{"value": "Larval", "label": "Larval", "count": 49, "toggle_url": "http://metadata.rnaquarium.org/metadata/run_metadata.json?experiment.library_source=TRANSCRIPTOMIC+SINGLE+CELL&tissue_curation_coarse=Liver+and+Biliary+System&devstage_curation_coarse=Larval", "selected": false}, {"value": "Adult", "label": "Adult", "count": 32, "toggle_url": "http://metadata.rnaquarium.org/metadata/run_metadata.json?experiment.library_source=TRANSCRIPTOMIC+SINGLE+CELL&tissue_curation_coarse=Liver+and+Biliary+System&devstage_curation_coarse=Adult", "selected": false}, {"value": "Undetermined", "label": "Undetermined", "count": 24, "toggle_url": "http://metadata.rnaquarium.org/metadata/run_metadata.json?experiment.library_source=TRANSCRIPTOMIC+SINGLE+CELL&tissue_curation_coarse=Liver+and+Biliary+System&devstage_curation_coarse=Undetermined", "selected": false}, {"value": "Embryo", "label": "Embryo", "count": 1, "toggle_url": "http://metadata.rnaquarium.org/metadata/run_metadata.json?experiment.library_source=TRANSCRIPTOMIC+SINGLE+CELL&tissue_curation_coarse=Liver+and+Biliary+System&devstage_curation_coarse=Embryo", "selected": false}], "truncated": false}, "devstage_curation": {"name": "devstage_curation", "type": "column", "hideable": false, "toggle_url": "/metadata/run_metadata.json?experiment.library_source=TRANSCRIPTOMIC+SINGLE+CELL&tissue_curation_coarse=Liver+and+Biliary+System", "results": [{"value": "Undetermined", "label": "Undetermined", "count": 68, "toggle_url": "http://metadata.rnaquarium.org/metadata/run_metadata.json?experiment.library_source=TRANSCRIPTOMIC+SINGLE+CELL&tissue_curation_coarse=Liver+and+Biliary+System&devstage_curation=Undetermined", "selected": false}, {"value": "Adult", "label": "Adult", "count": 32, "toggle_url": "http://metadata.rnaquarium.org/metadata/run_metadata.json?experiment.library_source=TRANSCRIPTOMIC+SINGLE+CELL&tissue_curation_coarse=Liver+and+Biliary+System&devstage_curation=Adult", "selected": false}, {"value": "Larval", "label": "Larval", "count": 5, "toggle_url": "http://metadata.rnaquarium.org/metadata/run_metadata.json?experiment.library_source=TRANSCRIPTOMIC+SINGLE+CELL&tissue_curation_coarse=Liver+and+Biliary+System&devstage_curation=Larval", "selected": false}, {"value": "Zygote", "label": "Zygote", "count": 1, "toggle_url": "http://metadata.rnaquarium.org/metadata/run_metadata.json?experiment.library_source=TRANSCRIPTOMIC+SINGLE+CELL&tissue_curation_coarse=Liver+and+Biliary+System&devstage_curation=Zygote", "selected": false}], "truncated": false}, "tissue_curation_coarse": {"name": "tissue_curation_coarse", "type": "column", "hideable": false, "toggle_url": "/metadata/run_metadata.json?experiment.library_source=TRANSCRIPTOMIC+SINGLE+CELL&tissue_curation_coarse=Liver+and+Biliary+System", "results": [{"value": "Liver and Biliary System", "label": "Liver and Biliary System", "count": 106, "toggle_url": "http://metadata.rnaquarium.org/metadata/run_metadata.json?experiment.library_source=TRANSCRIPTOMIC+SINGLE+CELL", "selected": true}], "truncated": false}, "tissue_curation": {"name": "tissue_curation", "type": "column", "hideable": false, "toggle_url": "/metadata/run_metadata.json?experiment.library_source=TRANSCRIPTOMIC+SINGLE+CELL&tissue_curation_coarse=Liver+and+Biliary+System", "results": [{"value": "Liver", "label": "Liver", "count": 106, "toggle_url": "http://metadata.rnaquarium.org/metadata/run_metadata.json?experiment.library_source=TRANSCRIPTOMIC+SINGLE+CELL&tissue_curation_coarse=Liver+and+Biliary+System&tissue_curation=Liver", "selected": false}], "truncated": false}, "technology": {"name": "technology", "type": "column", "hideable": false, "toggle_url": "/metadata/run_metadata.json?experiment.library_source=TRANSCRIPTOMIC+SINGLE+CELL&tissue_curation_coarse=Liver+and+Biliary+System", "results": [{"value": "10x", "label": "10x", "count": 78, "toggle_url": "http://metadata.rnaquarium.org/metadata/run_metadata.json?experiment.library_source=TRANSCRIPTOMIC+SINGLE+CELL&tissue_curation_coarse=Liver+and+Biliary+System&technology=10x", "selected": false}, {"value": "dropseq", "label": "dropseq", "count": 28, "toggle_url": "http://metadata.rnaquarium.org/metadata/run_metadata.json?experiment.library_source=TRANSCRIPTOMIC+SINGLE+CELL&tissue_curation_coarse=Liver+and+Biliary+System&technology=dropseq", "selected": false}], "truncated": false}}, "suggested_facets": [], "next": "74555", "next_url": "http://metadata.rnaquarium.org/metadata/run_metadata.json?experiment.library_source=TRANSCRIPTOMIC+SINGLE+CELL&tissue_curation_coarse=Liver+and+Biliary+System&_next=74555", "private": false, "allow_execute_sql": true, "query_ms": 114.40951499389485}