{"database": "metadata", "table": "run_metadata", "is_view": false, "human_description_en": "where experiment.library_source = \"TRANSCRIPTOMIC SINGLE CELL\", technology = \"generic-scrnaseq-only\" and tissue_curation = \"Brain\"", "rows": [[26536, "SRR26130973", "SRX21844394", "SRS18942411", "SRP462307", "PRJNA1019490", "Single Cell RNA seq of Zebrafish Hypothalamus", "PRJNA1019490", "Other", "Brain nuclei are traditionally defined by their anatomy  activity  and expression of specific markers. The hypothalamus contains discrete neuronal populations that coordinate fundamental behavioral functions  ranging from sleep and wakefulness to feeding  stress  and reward in all vertebrates.", null, null, null, null, "10X49 2", null, "breed:NA|age:Adult|collection date:2021|geo loc name:Israel|sex:not applicable|tissue:Brain Hypothalamus|sample type:single cell 6|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "SC ZF Hypo6", "10X49 2", "10X49 2", "we worked swiftly with cold  well oxygenated solutions  optimizing Papain incubation times for tissue age and brain region. We used caution with Ca2+ and Mg2+ in aCSF to avoid RNA extraction/RT interference  favoring Ca2+/Mg2+ free aCSF if not diluted downstream. Modified aCSF had specific component concentrations  and our materials included essential equipment and anesthesia. We prepared aCSF from an 8x stock with adjusted CaCl2 and MgSO4 concentrations  kept it fresh  and oxygenated it. The zebrafish procedure began with lethal anesthesia  brain dissection in ice cold aCSF  embedding in 1.5% gel  and vibratome sectioning. Tissue was dissociated in the Papain vial  triturated  filtered  and washed  followed by centrifugation. The pellet was resuspended in aCSF with DNase  assessed for cell viability  counted  and diluted for use.", null, null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "Oligo-dT", "PAIRED", "ILLUMINA", "Illumina HiSeq 2000", null, "SRP462307", null, null, "10X49_2_S5_L001_R1_001.fastq.gz 10X49_2_S5_L001_R2_001.fastq.gz 10X49_2_S5_L002_R1_001.fastq.gz 10X49_2_S5_L002_R2_001.fastq.gz 10X49_2_S5_L003_R1_001.fastq.gz 10X49_2_S5_L003_R2_001.fastq.gz 10X49_2_S5_L004_R1_001.fastq.gz 10X49_2_S5_L004_R2_001.fastq.gz", "fastq fastq fastq fastq fastq fastq fastq fastq", 3126113844.0, 37215641.0, "10X49 2 S5 L001 R1 001.fastq.gz", "0:28 1:56", "A:898881980;C:657306128;G:664563758;T:905262774;N:99204", 28, 56, null, null, 898881980, 657306128, 664563758, 905262774, 99204, "SRX21844394", "SRS18942411", "SRA1717104", "Technion - Israel Institute of Technology|Neuroscince", "Technion - Israel Institute of Technology", 2, 0.01241, 0.88203, 0.00542, 0.35268, 0.98129, 0.7586, 0.40274, 0.5074, 28, 56, "T", "B", "sc-like readlen", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "Israel", "2023-09-21", "Adult", "Adult", "Brain", "Nervous System"], [26537, "SRR26130974", "SRX21844393", "SRS18942407", "SRP462307", "PRJNA1019490", "Single Cell RNA seq of Zebrafish Hypothalamus", "PRJNA1019490", "Other", "Brain nuclei are traditionally defined by their anatomy  activity  and expression of specific markers. The hypothalamus contains discrete neuronal populations that coordinate fundamental behavioral functions  ranging from sleep and wakefulness to feeding  stress  and reward in all vertebrates.", null, null, null, null, "10X49 1", null, "breed:NA|age:Adult|collection date:2021|geo loc name:Israel|sex:not applicable|tissue:Brain Hypothalamus|sample type:single cell 5|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "SC ZF Hypo5", "10X49 1", "10X49 1", "we worked swiftly with cold  well oxygenated solutions  optimizing Papain incubation times for tissue age and brain region. We used caution with Ca2+ and Mg2+ in aCSF to avoid RNA extraction/RT interference  favoring Ca2+/Mg2+ free aCSF if not diluted downstream. Modified aCSF had specific component concentrations  and our materials included essential equipment and anesthesia. We prepared aCSF from an 8x stock with adjusted CaCl2 and MgSO4 concentrations  kept it fresh  and oxygenated it. The zebrafish procedure began with lethal anesthesia  brain dissection in ice cold aCSF  embedding in 1.5% gel  and vibratome sectioning. Tissue was dissociated in the Papain vial  triturated  filtered  and washed  followed by centrifugation. The pellet was resuspended in aCSF with DNase  assessed for cell viability  counted  and diluted for use.", null, null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "Oligo-dT", "PAIRED", "ILLUMINA", "Illumina HiSeq 2000", null, "SRP462307", null, null, "10X49_1_S4_L001_R1_001.fastq.gz 10X49_1_S4_L001_R2_001.fastq.gz 10X49_1_S4_L002_R1_001.fastq.gz 10X49_1_S4_L002_R2_001.fastq.gz 10X49_1_S4_L003_R1_001.fastq.gz 10X49_1_S4_L003_R2_001.fastq.gz 10X49_1_S4_L004_R1_001.fastq.gz 10X49_1_S4_L004_R2_001.fastq.gz", "fastq fastq fastq fastq fastq fastq fastq fastq", 3315364752.0, 39468628.0, "10X49 1 S4 L001 R1 001.fastq.gz", "0:28 1:56", "A:957164498;C:690985782;G:704099796;T:963009918;N:104758", 28, 56, null, null, 957164498, 690985782, 704099796, 963009918, 104758, "SRX21844393", "SRS18942407", "SRA1717104", "Technion - Israel Institute of Technology|Neuroscince", "Technion - Israel Institute of Technology", 2, 0.0143, 0.88325, 0.00601, 0.35854, 0.97816, 0.75868, 0.4158, 0.50825, 28, 56, "T", "B", "sc-like readlen", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "Israel", "2023-09-21", "Adult", "Adult", "Brain", "Nervous System"], [26538, "SRR26130975", "SRX21844392", "SRS18942399", "SRP462307", "PRJNA1019490", "Single Cell RNA seq of Zebrafish Hypothalamus", "PRJNA1019490", "Other", "Brain nuclei are traditionally defined by their anatomy  activity  and expression of specific markers. The hypothalamus contains discrete neuronal populations that coordinate fundamental behavioral functions  ranging from sleep and wakefulness to feeding  stress  and reward in all vertebrates.", null, null, null, null, "10X47 2", null, "breed:NA|age:Adult|collection date:2021|geo loc name:Israel|sex:not applicable|tissue:Brain Hypothalamus|sample type:single cell 4|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "SC ZF Hypo4", "10X47 2", "10X47 2", "we worked swiftly with cold  well oxygenated solutions  optimizing Papain incubation times for tissue age and brain region. We used caution with Ca2+ and Mg2+ in aCSF to avoid RNA extraction/RT interference  favoring Ca2+/Mg2+ free aCSF if not diluted downstream. Modified aCSF had specific component concentrations  and our materials included essential equipment and anesthesia. We prepared aCSF from an 8x stock with adjusted CaCl2 and MgSO4 concentrations  kept it fresh  and oxygenated it. The zebrafish procedure began with lethal anesthesia  brain dissection in ice cold aCSF  embedding in 1.5% gel  and vibratome sectioning. Tissue was dissociated in the Papain vial  triturated  filtered  and washed  followed by centrifugation. 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The hypothalamus contains discrete neuronal populations that coordinate fundamental behavioral functions  ranging from sleep and wakefulness to feeding  stress  and reward in all vertebrates.", null, null, null, null, "10X47 1", null, "breed:NA|age:Adult|collection date:2021|geo loc name:Israel|sex:not applicable|tissue:Brain Hypothalamus|sample type:single cell 3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "SC ZF Hypo3", "10X47 1", "10X47 1", "we worked swiftly with cold  well oxygenated solutions  optimizing Papain incubation times for tissue age and brain region. We used caution with Ca2+ and Mg2+ in aCSF to avoid RNA extraction/RT interference  favoring Ca2+/Mg2+ free aCSF if not diluted downstream. Modified aCSF had specific component concentrations  and our materials included essential equipment and anesthesia. We prepared aCSF from an 8x stock with adjusted CaCl2 and MgSO4 concentrations  kept it fresh  and oxygenated it. The zebrafish procedure began with lethal anesthesia  brain dissection in ice cold aCSF  embedding in 1.5% gel  and vibratome sectioning. Tissue was dissociated in the Papain vial  triturated  filtered  and washed  followed by centrifugation. 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The zebrafish procedure began with lethal anesthesia  brain dissection in ice cold aCSF  embedding in 1.5% gel  and vibratome sectioning. Tissue was dissociated in the Papain vial  triturated  filtered  and washed  followed by centrifugation. 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The zebrafish procedure began with lethal anesthesia  brain dissection in ice cold aCSF  embedding in 1.5% gel  and vibratome sectioning. Tissue was dissociated in the Papain vial  triturated  filtered  and washed  followed by centrifugation. 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