{"database": "metadata", "table": "run_metadata", "is_view": false, "human_description_en": "where experiment.library_source = \"TRANSCRIPTOMIC SINGLE CELL\", experiment.library_strategy = \"RNA-Seq\" and tissue_curation_coarse = \"Nervous System\"", "rows": [[10176, "ERR5858457", "ERX5504346", "ERS6343450", "ERP128749", "PRJEB44676", "scRNAseq of her4.3+ cells from lesioned and unlesioned zebrafish larvae spinal cord", "E-MTAB-10390", "Transcriptome Analysis", "To analyse lesion induced gene regulation in progenitor cells at single cell resolution  we performed single cell RNAseq on FACS isolated her4.3:GFP progenitor cells from the spinal cord at 24 hours post lesion hpl post spinal injury at 3 dpf dpf  compared to age matched uninjured animals.", "ENA FIRST PUBLIC:2021 05 24|ENA LAST UPDATE:2021 05 24", null, "Protocols: Trunks containing the lesion sites or equivalent site from unlesioned fish are collected and kept in PBS on ice.Incubate the trunks up to 300 in 1 mL of 1X Trypsin EDTA at 37C for 5 7 mins.Stop dissociation by adding FBS to a final concentration of 5%.Centrifuge at 200g for 7 mins.Discard Sups.Resuspend in 500 uL of PBS.Add the cell suspension into a 40 uM cell strainer.Centrifuge at 200g for 7 mins.Resuspend in the buffer for FACS PBS 5% FBS Following cell dissociation and FAC sorting  samples were processed on the 10X Chromium platform using 10X Single Cell three prime v3 chemistry following the manufacturer's guidelines Following cell dissociation and FAC sorting  samples were processed on the 10X Chromium platform using 10X Single Cell three prime v3 chemistry following the manufacturer's guidelines.", "Naive", "SAMEA8658904", "University Of Edinburgh", "ENA first public:2021 05 24|ENA last update:2021 05 24|External Id:SAMEA8658904|INSDC center alias:UOE|INSDC center name:University Of Edinburgh|INSDC first public:2021 05 24T00:14:32Z|INSDC last update:2021 05 24T00:14:32Z|INSDC status:public|Submitter Id:E MTAB 10390:Naive|age:4|broker name:ArrayExpress|cell type:ependymo radial glial cell|common name:zebrafish|developmental stage:larval day 4|immunophenotype:Her4.3+ positive|organism part:spinal cord|sample name:E MTAB 10390:Naive|sex:mixed|strain:WIK", null, null, null, null, null, null, null, null, "Illumina NovaSeq 6000 paired end sequencing; scRNAseq of her4.3+ cells from lesi1d and unlesi1d zebrafish larvae spinal cord", "E MTAB 10390:Naive p", "Naive p", "scRNAseq of her4.3+ cells from lesioned and unlesioned zebrafish larvae spinal cord", "Trunks containing the lesion sites or equivalent site from unlesioned fish are collected and kept in PBS on ice.Incubate the trunks up to 300 in 1 mL of 1X Trypsin EDTA at 37C for 5 7 mins.Stop dissociation by adding FBS to a final concentration of 5%.Centrifuge at 200g for 7 mins.Discard Sups.Resuspend in 500 uL of PBS.Add the cell suspension into a 40 uM cell strainer.Centrifuge at 200g for 7 mins.Resuspend in the buffer for FACS PBS 5% FBS Following cell dissociation and FAC sorting  samples were processed on the 10X Chromium platform using 10X Single Cell three prime v3 chemistry following the manufacturer's guidelines  Following cell dissociation and FAC sorting  samples were processed on the 10X Chromium platform using 10X Single Cell three prime v3 chemistry following the manufacturer's guidelines.", "Experimental Factor: injury:n1", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "Oligo-dT", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP128749", "Illumina NovaSeq 6000 paired end sequencing; scRNAseq of her4.3+ cells from lesioned and unlesioned zebrafish larvae spinal cord", "ENA FIRST PUBLIC:2021 05 24|ENA LAST UPDATE:2021 05 24", "Naive.bam", "bam", 44972162730.0, 499690697.0, "E MTAB 10390:Naive", "0:90", "A:13658826375;C:8733304343;G:9348137569;T:13228064343;N:3830100", 90, null, null, null, 13658826375, 8733304343, 9348137569, 13228064343, 3830100, "ERX5504346", "ERS6343450", "ERA4142789", "University Of Edinburgh|European Nucleotide Archive", "University Of Edinburgh|European Nucleotide Archive", 1, 0.89428, null, 0.32693, null, 0.75276, null, 0.5314, null, 90, null, "B", null, "usable mapping rate", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_droplet", "10x", null, "United Kingdom", "2021-05-24", "Larval", "Larval", "Spinal Cord", "Nervous System"], [11813, "ERR11788863", "ERX11187331", "ERS16221143", "ERP149944", "PRJEB64780", "A single cell transcriptomic atlas reveals a new myeloid parenchymal population in the zebrafish brain", "E-MTAB-13223", "Transcriptome Analysis", "To understand the cellular basis of the immune compartment of the zebrafish brain we have established reliable protocols for dissociation and prospective isolation of brain leukocytes  using fluorescent transgenic lines. By combining this approach with single cell RNA sequencing  we have generated a gene expression atlas composed of the distinct immune cells present in the homeostatic brain. These analyses revealed the presence of subpopulations of mononuclear phagocytes and other leukocytes  including cell types that have not been  or have been poorly  characterized so far. Here  we present the characterization of a new mononuclear phagocyte population that represents an important fraction among all brain leukocytes.  Adult brain single cell suspensions were prepared from adult Tgp2ry12:GFP; cd45:DsRed fish n=3 and a total of 14 000 cd45:DsRed+ cells were processed for single cell profiling using the 10x Genomics platform.", "ENA FIRST PUBLIC:2023 12 25|ENA LAST UPDATE:2023 12 25", null, "Protocols: FACS sorting of cd45:Dsred cells. Brains from adult Tgp2ry12:GFP; cd45:DsRed fish n=3  were dissected in 0.9X Dulbecco\u2032s Phosphate Buffered Saline DPBS were triturated and treated with Liberase TM at 33\u00b0C for 30 45 minutes  fully dissociated using a syringe with a 26G needle and washed in 2% fetal bovine serum diluted in 0.9X DPBS. Cell suspensions were centrifuged at 290g 4\u00baC 10 min and filtered through a 40\u00b5m nylon mesh. Just before flow cytometry analysis  using calcein violet to exclude dead cells 1\u00b5M  Thermo Fisher. Flow cytometry acquisition and cell sorting was performed on a FACS ARIA II Becton Dickinson. RNA Illumina", "Sample 1", "SAMEA114235870", "Universite Libre de Bruxelles", "ENA FIRST PUBLIC:2023 12 25T01:15:29Z|ENA LAST UPDATE:2023 12 25T01:15:29Z|External Id:SAMEA114235870|INSDC center name:Universite Libre de Bruxelles|INSDC first public:2023 12 25T01:15:29Z|INSDC last update:2023 12 25T01:15:29Z|INSDC status:public|Submitter Id:E MTAB 13223:Sample 1|age:5|broker name:ArrayExpress|cell type:leukocyte|collection date:not collected|common name:zebrafish|developmental stage:adult|genotype:wild type genotype|geographic location country and/or sea:not collected|individual:pool of 3 fish|isolate:not applicable|organism part:brain|sample name:E MTAB 13223:Sample 1|scientific name:Danio rerio|sex:male and female|strain:AB", null, null, null, null, null, null, null, null, "A single cell transcriptomic atlas reveals a new myeloid parenchymal population in the zebrafish brain", "E MTAB 13223:Sample 1 p", "Sample 1 p", "A single cell transcriptomic atlas reveals a new myeloid parenchymal population in the zebrafish brain", "FACS sorting of cd45:Dsred cells. Brains from adult Tgp2ry12:GFP; cd45:DsRed fish n=3  were dissected in 0.9X Dulbecco\u2032s Phosphate Buffered Saline DPBS were triturated and treated with Liberase TM at 33\u00b0C for 30 45 minutes  fully dissociated using a syringe with a 26G needle and washed in 2% fetal bovine serum diluted in 0.9X DPBS. Cell suspensions were centrifuged at 290g 4\u00baC 10 min and filtered through a 40\u00b5m nylon mesh. Just before flow cytometry analysis  using calcein violet to exclude dead cells 1\u00b5M  Thermo Fisher. Flow cytometry acquisition and cell sorting was performed on a FACS ARIA II Becton Dickinson.  RNA Illumina", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "PolyA", "PAIRED", "ILLUMINA", "NextSeq 550", null, "ERP149944", "NextSeq 550 paired end sequencing; A single cell transcriptomic atlas reveals a new myeloid parenchymal population in the zebrafish brain", "ENA FIRST PUBLIC:2023 12 25|ENA LAST UPDATE:2023 12 25", "L41805_Track-81843_R1.fastq.gz L41805_Track-81843_R2.fastq.gz", "fastq fastq", 5545444464.0, 66017196.0, "E MTAB 13223:L41805 Track 81843", "0:28 1:56", "A:1576174024;C:1193175858;G:1221776615;T:1551009004;N:3308963", 28, 56, null, null, 1576174024, 1193175858, 1221776615, 1551009004, 3308963, "ERX11187331", "ERS16221143", "ERA26318932", "European Bioinformatics Institute|European Nucleotide Archive", "European Bioinformatics Institute|European Nucleotide Archive", 2, 0.00738, 0.90151, 0.00285, 0.20553, 0.99072, 0.82402, 0.4645, 0.53817, 28, 56, "T", "B", "sc-like readlen", "illumina", "nextseq", "unknown", "poly_a", "unknown", "sc", "single_cell_droplet", "10x", null, "United Kingdom", "2023-12-25", "Adult", "Adult", "Brain", "Nervous System"], [11814, "ERR11788864", "ERX11187331", "ERS16221143", "ERP149944", "PRJEB64780", "A single cell transcriptomic atlas reveals a new myeloid parenchymal population in the zebrafish brain", "E-MTAB-13223", "Transcriptome Analysis", "To understand the cellular basis of the immune compartment of the zebrafish brain we have established reliable protocols for dissociation and prospective isolation of brain leukocytes  using fluorescent transgenic lines. By combining this approach with single cell RNA sequencing  we have generated a gene expression atlas composed of the distinct immune cells present in the homeostatic brain. These analyses revealed the presence of subpopulations of mononuclear phagocytes and other leukocytes  including cell types that have not been  or have been poorly  characterized so far. Here  we present the characterization of a new mononuclear phagocyte population that represents an important fraction among all brain leukocytes.  Adult brain single cell suspensions were prepared from adult Tgp2ry12:GFP; cd45:DsRed fish n=3 and a total of 14 000 cd45:DsRed+ cells were processed for single cell profiling using the 10x Genomics platform.", "ENA FIRST PUBLIC:2023 12 25|ENA LAST UPDATE:2023 12 25", null, "Protocols: FACS sorting of cd45:Dsred cells. Brains from adult Tgp2ry12:GFP; cd45:DsRed fish n=3  were dissected in 0.9X Dulbecco\u2032s Phosphate Buffered Saline DPBS were triturated and treated with Liberase TM at 33\u00b0C for 30 45 minutes  fully dissociated using a syringe with a 26G needle and washed in 2% fetal bovine serum diluted in 0.9X DPBS. Cell suspensions were centrifuged at 290g 4\u00baC 10 min and filtered through a 40\u00b5m nylon mesh. Just before flow cytometry analysis  using calcein violet to exclude dead cells 1\u00b5M  Thermo Fisher. Flow cytometry acquisition and cell sorting was performed on a FACS ARIA II Becton Dickinson. RNA Illumina", "Sample 1", "SAMEA114235870", "Universite Libre de Bruxelles", "ENA FIRST PUBLIC:2023 12 25T01:15:29Z|ENA LAST UPDATE:2023 12 25T01:15:29Z|External Id:SAMEA114235870|INSDC center name:Universite Libre de Bruxelles|INSDC first public:2023 12 25T01:15:29Z|INSDC last update:2023 12 25T01:15:29Z|INSDC status:public|Submitter Id:E MTAB 13223:Sample 1|age:5|broker name:ArrayExpress|cell type:leukocyte|collection date:not collected|common name:zebrafish|developmental stage:adult|genotype:wild type genotype|geographic location country and/or sea:not collected|individual:pool of 3 fish|isolate:not applicable|organism part:brain|sample name:E MTAB 13223:Sample 1|scientific name:Danio rerio|sex:male and female|strain:AB", null, null, null, null, null, null, null, null, "A single cell transcriptomic atlas reveals a new myeloid parenchymal population in the zebrafish brain", "E MTAB 13223:Sample 1 p", "Sample 1 p", "A single cell transcriptomic atlas reveals a new myeloid parenchymal population in the zebrafish brain", "FACS sorting of cd45:Dsred cells. Brains from adult Tgp2ry12:GFP; cd45:DsRed fish n=3  were dissected in 0.9X Dulbecco\u2032s Phosphate Buffered Saline DPBS were triturated and treated with Liberase TM at 33\u00b0C for 30 45 minutes  fully dissociated using a syringe with a 26G needle and washed in 2% fetal bovine serum diluted in 0.9X DPBS. Cell suspensions were centrifuged at 290g 4\u00baC 10 min and filtered through a 40\u00b5m nylon mesh. Just before flow cytometry analysis  using calcein violet to exclude dead cells 1\u00b5M  Thermo Fisher. Flow cytometry acquisition and cell sorting was performed on a FACS ARIA II Becton Dickinson.  RNA Illumina", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "PolyA", "PAIRED", "ILLUMINA", "NextSeq 550", null, "ERP149944", "NextSeq 550 paired end sequencing; A single cell transcriptomic atlas reveals a new myeloid parenchymal population in the zebrafish brain", "ENA FIRST PUBLIC:2023 12 25|ENA LAST UPDATE:2023 12 25", "L41805_Track-81377_R1.fastq.gz L41805_Track-81377_R2.fastq.gz", "fastq fastq", 3184137096.0, 37906394.0, "E MTAB 13223:L41805 Track 81377", "0:28 1:56", "A:899943184;C:685571078;G:703849582;T:889228559;N:5544693", 28, 56, null, null, 899943184, 685571078, 703849582, 889228559, 5544693, "ERX11187331", "ERS16221143", "ERA26318932", "European Bioinformatics Institute|European Nucleotide Archive", "European Bioinformatics Institute|European Nucleotide Archive", 2, 0.00734, 0.90077, 0.00293, 0.20318, 0.99064, 0.82765, 0.47994, 0.53473, 28, 56, "T", "B", "sc-like readlen", "illumina", "nextseq", "unknown", "poly_a", "unknown", "sc", "single_cell_droplet", "10x", null, "United Kingdom", "2023-12-25", "Adult", "Adult", "Brain", "Nervous System"], [19221, "ERR14935135", "ERX14339377", "ERS24360099", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well H06", "Humanized Plate 1   Well H06", "SAMEA118225022", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample HUM1 H06|collection date:2022 11 14|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample HUM1 H06|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample HUM1 H06", "webin reads Sample HUM1 H06", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample HUM1 H06", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "H06.R1.fastq.gz H06.R2.fastq.gz", "fastq fastq", 168135000.0, 560450.0, "webin reads Sample HUM1 H06", "0:150 1:150", "A:49336993;C:19637394;G:50002867;T:49156187;N:1559", 150, 150, null, null, 49336993, 19637394, 50002867, 49156187, 1559, "ERX14339377", "ERS24360099", "ERA33113052", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "mate1-mate2 similar by mapping diff", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19222, "ERR14934924", "ERX14339166", "ERS24360038", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well C05", "Humanized Plate 1   Well C05", "SAMEA118224961", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample HUM1 C05|collection date:2022 11 14|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample HUM1 C05|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample HUM1 C05", "webin reads Sample HUM1 C05", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample HUM1 C05", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "C05.R1.fastq.gz C05.R2.fastq.gz", "fastq fastq", 406863000.0, 1356210.0, "webin reads Sample HUM1 C05", "0:150 1:150", "A:135043675;C:48707343;G:87607964;T:135501103;N:2915", 150, 150, null, null, 135043675, 48707343, 87607964, 135501103, 2915, "ERX14339166", "ERS24360038", "ERA33112290", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "mate1-mate2 similar by mapping diff", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19223, "ERR14934974", "ERX14339216", "ERS24360047", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well D02", "Humanized Plate 1   Well D02", "SAMEA118224970", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample HUM1 D02|collection date:2022 11 14|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample HUM1 D02|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample HUM1 D02", "webin reads Sample HUM1 D02", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample HUM1 D02", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "D02.R1.fastq.gz D02.R2.fastq.gz", "fastq fastq", 231350700.0, 771169.0, "webin reads Sample HUM1 D02", "0:150 1:150", "A:61554211;C:27195025;G:82686912;T:59912853;N:1699", 150, 150, null, null, 61554211, 27195025, 82686912, 59912853, 1699, "ERX14339216", "ERS24360047", "ERA33112492", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "T", "T", "mates < 9% mapping rate", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19224, "ERR14937148", "ERX14341392", "ERS24422818", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well G07", "Humanized Plate 2   Well G07", "SAMEA118242644", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample HUM2 G07|collection date:2022 12 21|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample HUM2 G07|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample HUM2 G07", "webin reads Sample HUM2 G07", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample HUM2 G07", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "G07.R1.fastq.gz G07.R2.fastq.gz", "fastq fastq", 1858827300.0, 6196091.0, "webin reads Sample HUM2 G07", "0:150 1:150", "A:377243729;C:275647713;G:834077378;T:371849924;N:8556", 150, 150, null, null, 377243729, 275647713, 834077378, 371849924, 8556, "ERX14341392", "ERS24422818", "ERA33115718", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "T", "T", "mates < 9% mapping rate", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19225, "ERR14935143", "ERX14339385", "ERS24360101", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well H08", "Humanized Plate 1   Well H08", "SAMEA118225024", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample HUM1 H08|collection date:2022 11 14|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample HUM1 H08|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample HUM1 H08", "webin reads Sample HUM1 H08", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample HUM1 H08", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "H08.R1.fastq.gz H08.R2.fastq.gz", "fastq fastq", 104513100.0, 348377.0, "webin reads Sample HUM1 H08", "0:150 1:150", "A:24551652;C:12552824;G:43587381;T:23820238;N:1005", 150, 150, null, null, 24551652, 12552824, 43587381, 23820238, 1005, "ERX14339385", "ERS24360101", "ERA33113082", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "T", "T", "mates < 9% mapping rate", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19226, "ERR14936904", "ERX14341146", "ERS24422763", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well B12", "Humanized Plate 2   Well B12", "SAMEA118242589", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample HUM2 B12|collection date:2022 12 21|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample HUM2 B12|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample HUM2 B12", "webin reads Sample HUM2 B12", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample HUM2 B12", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "B12.R1.fastq.gz B12.R2.fastq.gz", "fastq fastq", 14783024100.0, 49276747.0, "webin reads Sample HUM2 B12", "0:150 1:150", "A:4993941125;C:1900193626;G:3694935207;T:4193880937;N:73205", 150, 150, null, null, 4993941125, 1900193626, 3694935207, 4193880937, 73205, "ERX14341146", "ERS24422763", "ERA33115587", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "T", "T", "mates < 9% mapping rate", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19227, "ERR14935120", "ERX14339362", "ERS24360094", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well H01", "Humanized Plate 1   Well H01", "SAMEA118225017", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample HUM1 H01|collection date:2022 11 14|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample HUM1 H01|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample HUM1 H01", "webin reads Sample HUM1 H01", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample HUM1 H01", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "H01.R1.fastq.gz H01.R2.fastq.gz", "fastq fastq", 214281900.0, 714273.0, "webin reads Sample HUM1 H01", "0:150 1:150", "A:63612930;C:24945238;G:64455038;T:61267490;N:1204", 150, 150, null, null, 63612930, 24945238, 64455038, 61267490, 1204, "ERX14339362", "ERS24360094", "ERA33112989", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19228, "ERR14935021", "ERX14339263", "ERS24360064", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well E07", "Humanized Plate 1   Well E07", "SAMEA118224987", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample HUM1 E07|collection date:2022 11 14|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample HUM1 E07|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample HUM1 E07", "webin reads Sample HUM1 E07", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample HUM1 E07", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "E07.R1.fastq.gz E07.R2.fastq.gz", "fastq fastq", 274863600.0, 916212.0, "webin reads Sample HUM1 E07", "0:150 1:150", "A:89634361;C:31803602;G:65721376;T:87702420;N:1841", 150, 150, null, null, 89634361, 31803602, 65721376, 87702420, 1841, "ERX14339263", "ERS24360064", "ERA33112659", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "mate1-mate2 similar by mapping diff", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19229, "ERR14950666", "ERX14354790", "ERS24422897", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well C09", "Knockout Plate   Well C09", "SAMEA118242723", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample KO C09|collection date:2022 05 13|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample KO C09|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample KO C09", "webin reads Sample KO C09", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample KO C09", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "C09.R1.fastq.gz C09.R2.fastq.gz", "fastq fastq", 12910892700.0, 43036309.0, "webin reads Sample KO C09", "0:150 1:150", "A:4656205100;C:1791783755;G:2065221420;T:4397579948;N:102477", 150, 150, null, null, 4656205100, 1791783755, 2065221420, 4397579948, 102477, "ERX14354790", "ERS24422897", "ERA33121161", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19230, "ERR14936878", "ERX14341120", "ERS24422755", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well B04", "Humanized Plate 2   Well B04", "SAMEA118242581", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample HUM2 B04|collection date:2022 12 21|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample HUM2 B04|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample HUM2 B04", "webin reads Sample HUM2 B04", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample HUM2 B04", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "B04.R1.fastq.gz B04.R2.fastq.gz", "fastq fastq", 2614344300.0, 8714481.0, "webin reads Sample HUM2 B04", "0:150 1:150", "A:495532634;C:388504084;G:1222436337;T:507858943;N:12302", 150, 150, null, null, 495532634, 388504084, 1222436337, 507858943, 12302, "ERX14341120", "ERS24422755", "ERA33115558", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "T", "T", "mates < 9% mapping rate", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19231, "ERR14950561", "ERX14354685", "ERS24422871", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well A07", "Knockout Plate   Well A07", "SAMEA118242697", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample KO A07|collection date:2022 05 13|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample KO A07|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample KO A07", "webin reads Sample KO A07", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample KO A07", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "A07.R1.fastq.gz A07.R2.fastq.gz", "fastq fastq", 3401128800.0, 11337096.0, "webin reads Sample KO A07", "0:150 1:150", "A:1234869505;C:459023584;G:579824655;T:1127383390;N:27666", 150, 150, null, null, 1234869505, 459023584, 579824655, 1127383390, 27666, "ERX14354685", "ERS24422871", "ERA33121056", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19232, "ERR14936823", "ERX14341065", "ERS24422746", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well A07", "Humanized Plate 2   Well A07", "SAMEA118242572", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample HUM2 A07|collection date:2022 12 21|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample HUM2 A07|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample HUM2 A07", "webin reads Sample HUM2 A07", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample HUM2 A07", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "A07.R1.fastq.gz A07.R2.fastq.gz", "fastq fastq", 3778502100.0, 12595007.0, "webin reads Sample HUM2 A07", "0:150 1:150", "A:710336891;C:494217522;G:1880968771;T:692961837;N:17079", 150, 150, null, null, 710336891, 494217522, 1880968771, 692961837, 17079, "ERX14341065", "ERS24422746", "ERA33115511", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "T", "T", "mates < 9% mapping rate", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19233, "ERR14937143", "ERX14341387", "ERS24422813", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well G02", "Humanized Plate 2   Well G02", "SAMEA118242639", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample HUM2 G02|collection date:2022 12 21|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample HUM2 G02|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample HUM2 G02", "webin reads Sample HUM2 G02", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample HUM2 G02", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "G02.R1.fastq.gz G02.R2.fastq.gz", "fastq fastq", 5962135800.0, 19873786.0, "webin reads Sample HUM2 G02", "0:150 1:150", "A:1745436521;C:821720129;G:1863789129;T:1531162608;N:27413", 150, 150, null, null, 1745436521, 821720129, 1863789129, 1531162608, 27413, "ERX14341387", "ERS24422813", "ERA33115710", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "T", "T", "mates < 9% mapping rate", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19234, "ERR14934810", "ERX14339052", "ERS24360011", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well A02", "Humanized Plate 1   Well A02", "SAMEA118224934", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample HUM1 A02|collection date:2022 11 14|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample HUM1 A02|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample HUM1 A02", "webin reads Sample HUM1 A02", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample HUM1 A02", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "A02.R1.fastq.gz A02.R2.fastq.gz", "fastq fastq", 480559200.0, 1601864.0, "webin reads Sample HUM1 A02", "0:150 1:150", "A:163177108;C:53718756;G:98835089;T:164825485;N:2762", 150, 150, null, null, 163177108, 53718756, 98835089, 164825485, 2762, "ERX14339052", "ERS24360011", "ERA33111955", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "mate1-mate2 similar by mapping diff", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19235, "ERR14934984", "ERX14339226", "ERS24360049", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well D04", "Humanized Plate 1   Well D04", "SAMEA118224972", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample HUM1 D04|collection date:2022 11 14|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample HUM1 D04|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample HUM1 D04", "webin reads Sample HUM1 D04", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample HUM1 D04", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "D04.R1.fastq.gz D04.R2.fastq.gz", "fastq fastq", 179225700.0, 597419.0, "webin reads Sample HUM1 D04", "0:150 1:150", "A:45457519;C:22876690;G:67395901;T:43493765;N:1825", 150, 150, null, null, 45457519, 22876690, 67395901, 43493765, 1825, "ERX14339226", "ERS24360049", "ERA33112537", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19236, "ERR14934845", "ERX14339087", "ERS24360021", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well A12", "Humanized Plate 1   Well A12", "SAMEA118224944", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample HUM1 A12|collection date:2022 11 14|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample HUM1 A12|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample HUM1 A12", "webin reads Sample HUM1 A12", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample HUM1 A12", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "A12.R1.fastq.gz A12.R2.fastq.gz", "fastq fastq", 77425200.0, 258084.0, "webin reads Sample HUM1 A12", "0:150 1:150", "A:16644810;C:10391814;G:34720928;T:15667259;N:389", 150, 150, null, null, 16644810, 10391814, 34720928, 15667259, 389, "ERX14339087", "ERS24360021", "ERA33112104", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "T", "T", "mates < 9% mapping rate", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19237, "ERR14934855", "ERX14339097", "ERS24360024", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well B03", "Humanized Plate 1   Well B03", "SAMEA118224947", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample HUM1 B03|collection date:2022 11 14|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample HUM1 B03|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample HUM1 B03", "webin reads Sample HUM1 B03", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample HUM1 B03", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "B03.R1.fastq.gz B03.R2.fastq.gz", "fastq fastq", 94155000.0, 313850.0, "webin reads Sample HUM1 B03", "0:150 1:150", "A:23101292;C:11479599;G:38484223;T:21089074;N:812", 150, 150, null, null, 23101292, 11479599, 38484223, 21089074, 812, "ERX14339097", "ERS24360024", "ERA33112137", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "T", "T", "mates < 9% mapping rate", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19238, "ERR14943975", "ERX14348127", "ERS24422869", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well A05", "Knockout Plate   Well A05", "SAMEA118242695", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample KO A05|collection date:2022 05 13|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample KO A05|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample KO A05", "webin reads Sample KO A05", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample KO A05", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "A05.R1.fastq.gz A05.R2.fastq.gz", "fastq fastq", 3911878200.0, 13039594.0, "webin reads Sample KO A05", "0:150 1:150", "A:1402938003;C:529582691;G:715316068;T:1264010327;N:31111", 150, 150, null, null, 1402938003, 529582691, 715316068, 1264010327, 31111, "ERX14348127", "ERS24422869", "ERA33119600", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19239, "ERR14937117", "ERX14341361", "ERS24422810", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well F11", "Humanized Plate 2   Well F11", "SAMEA118242636", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample HUM2 F11|collection date:2022 12 21|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample HUM2 F11|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample HUM2 F11", "webin reads Sample HUM2 F11", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample HUM2 F11", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "F11.R1.fastq.gz F11.R2.fastq.gz", "fastq fastq", 3943764300.0, 13145881.0, "webin reads Sample HUM2 F11", "0:150 1:150", "A:1194917338;C:577472498;G:1192413538;T:978942667;N:18259", 150, 150, null, null, 1194917338, 577472498, 1192413538, 978942667, 18259, "ERX14341361", "ERS24422810", "ERA33115700", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "T", "T", "mates < 9% mapping rate", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19240, "ERR14935149", "ERX14339391", "ERS24360104", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well H11", "Humanized Plate 1   Well H11", "SAMEA118225027", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample HUM1 H11|collection date:2022 11 14|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample HUM1 H11|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample HUM1 H11", "webin reads Sample HUM1 H11", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample HUM1 H11", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "H11.R1.fastq.gz H11.R2.fastq.gz", "fastq fastq", 29087400.0, 96958.0, "webin reads Sample HUM1 H11", "0:150 1:150", "A:5335277;C:3932942;G:14621885;T:5197119;N:177", 150, 150, null, null, 5335277, 3932942, 14621885, 5197119, 177, "ERX14339391", "ERS24360104", "ERA33113103", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "T", "T", "mates < 9% mapping rate", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19241, "ERR14935025", "ERX14339267", "ERS24360065", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well E08", "Humanized Plate 1   Well E08", "SAMEA118224988", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample HUM1 E08|collection date:2022 11 14|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample HUM1 E08|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample HUM1 E08", "webin reads Sample HUM1 E08", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample HUM1 E08", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "E08.R1.fastq.gz E08.R2.fastq.gz", "fastq fastq", 220002300.0, 733341.0, "webin reads Sample HUM1 E08", "0:150 1:150", "A:62013327;C:25647839;G:72394925;T:59944865;N:1344", 150, 150, null, null, 62013327, 25647839, 72394925, 59944865, 1344, "ERX14339267", "ERS24360065", "ERA33112670", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "T", "T", "mates < 9% mapping rate", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19242, "ERR14935108", "ERX14339350", "ERS24360090", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well G09", "Humanized Plate 1   Well G09", "SAMEA118225013", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample HUM1 G09|collection date:2022 11 14|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample HUM1 G09|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample HUM1 G09", "webin reads Sample HUM1 G09", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample HUM1 G09", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "G09.R1.fastq.gz G09.R2.fastq.gz", "fastq fastq", 55286400.0, 184288.0, "webin reads Sample HUM1 G09", "0:150 1:150", "A:9253015;C:6978214;G:29393822;T:9661042;N:307", 150, 150, null, null, 9253015, 6978214, 29393822, 9661042, 307, "ERX14339350", "ERS24360090", "ERA33112953", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "T", "T", "mates < 9% mapping rate", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19243, "ERR14935106", "ERX14339348", "ERS24360089", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well G08", "Humanized Plate 1   Well G08", "SAMEA118225012", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample HUM1 G08|collection date:2022 11 14|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample HUM1 G08|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample HUM1 G08", "webin reads Sample HUM1 G08", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample HUM1 G08", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "G08.R1.fastq.gz G08.R2.fastq.gz", "fastq fastq", 90390000.0, 301300.0, "webin reads Sample HUM1 G08", "0:150 1:150", "A:14519869;C:11004100;G:49374862;T:15490478;N:691", 150, 150, null, null, 14519869, 11004100, 49374862, 15490478, 691, "ERX14339348", "ERS24360089", "ERA33112948", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "T", "T", "mates < 9% mapping rate", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19244, "ERR14934877", "ERX14339119", "ERS24360032", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well B11", "Humanized Plate 1   Well B11", "SAMEA118224955", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample HUM1 B11|collection date:2022 11 14|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample HUM1 B11|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample HUM1 B11", "webin reads Sample HUM1 B11", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample HUM1 B11", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "B11.R1.fastq.gz B11.R2.fastq.gz", "fastq fastq", 99908700.0, 333029.0, "webin reads Sample HUM1 B11", "0:150 1:150", "A:22260827;C:11949991;G:42744522;T:22952623;N:737", 150, 150, null, null, 22260827, 11949991, 42744522, 22952623, 737, "ERX14339119", "ERS24360032", "ERA33112207", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "T", "T", "mates < 9% mapping rate", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19245, "ERR14934885", "ERX14339127", "ERS24360034", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well C01", "Humanized Plate 1   Well C01", "SAMEA118224957", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample HUM1 C01|collection date:2022 11 14|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample HUM1 C01|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample HUM1 C01", "webin reads Sample HUM1 C01", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample HUM1 C01", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "C01.R1.fastq.gz C01.R2.fastq.gz", "fastq fastq", 101124300.0, 337081.0, "webin reads Sample HUM1 C01", "0:150 1:150", "A:21597244;C:13549354;G:45547786;T:20429406;N:510", 150, 150, null, null, 21597244, 13549354, 45547786, 20429406, 510, "ERX14339127", "ERS24360034", "ERA33112235", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "T", "T", "mates < 9% mapping rate", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19246, "ERR14943984", "ERX14348136", "ERS24422880", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well B04", "Knockout Plate   Well B04", "SAMEA118242706", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample KO B04|collection date:2022 05 13|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample KO B04|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample KO B04", "webin reads Sample KO B04", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample KO B04", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "B04.R1.fastq.gz B04.R2.fastq.gz", "fastq fastq", 561237900.0, 1870793.0, "webin reads Sample KO B04", "0:150 1:150", "A:192169170;C:77013107;G:120338699;T:171712356;N:4568", 150, 150, null, null, 192169170, 77013107, 120338699, 171712356, 4568, "ERX14348136", "ERS24422880", "ERA33119691", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19247, "ERR14938992", "ERX14343236", "ERS24422834", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well H11", "Humanized Plate 2   Well H11", "SAMEA118242660", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample HUM2 H11|collection date:2022 12 21|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample HUM2 H11|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample HUM2 H11", "webin reads Sample HUM2 H11", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample HUM2 H11", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "H11.R1.fastq.gz H11.R2.fastq.gz", "fastq fastq", 1733814900.0, 5779383.0, "webin reads Sample HUM2 H11", "0:150 1:150", "A:378393702;C:284557462;G:721428373;T:349427441;N:7922", 150, 150, null, null, 378393702, 284557462, 721428373, 349427441, 7922, "ERX14343236", "ERS24422834", "ERA33115755", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "T", "T", "mates < 9% mapping rate", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19248, "ERR14937159", "ERX14341403", "ERS24422829", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well H06", "Humanized Plate 2   Well H06", "SAMEA118242655", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample HUM2 H06|collection date:2022 12 21|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample HUM2 H06|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample HUM2 H06", "webin reads Sample HUM2 H06", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample HUM2 H06", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "H06.R1.fastq.gz H06.R2.fastq.gz", "fastq fastq", 8840167200.0, 29467224.0, "webin reads Sample HUM2 H06", "0:150 1:150", "A:3070163952;C:1202397032;G:1876790493;T:2690771671;N:44052", 150, 150, null, null, 3070163952, 1202397032, 1876790493, 2690771671, 44052, "ERX14341403", "ERS24422829", "ERA33115733", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "mate1-mate2 similar by mapping diff", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19249, "ERR14934852", "ERX14339094", "ERS24360023", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well B02", "Humanized Plate 1   Well B02", "SAMEA118224946", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample HUM1 B02|collection date:2022 11 14|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample HUM1 B02|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample HUM1 B02", "webin reads Sample HUM1 B02", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample HUM1 B02", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "B02.R1.fastq.gz B02.R2.fastq.gz", "fastq fastq", 123106800.0, 410356.0, "webin reads Sample HUM1 B02", "0:150 1:150", "A:24870369;C:15859782;G:60046944;T:22329104;N:601", 150, 150, null, null, 24870369, 15859782, 60046944, 22329104, 601, "ERX14339094", "ERS24360023", "ERA33112126", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "T", "T", "mates < 9% mapping rate", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19250, "ERR14936902", "ERX14341144", "ERS24422761", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well B10", "Humanized Plate 2   Well B10", "SAMEA118242587", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample HUM2 B10|collection date:2022 12 21|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample HUM2 B10|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample HUM2 B10", "webin reads Sample HUM2 B10", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample HUM2 B10", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "B10.R1.fastq.gz B10.R2.fastq.gz", "fastq fastq", 3818756100.0, 12729187.0, "webin reads Sample HUM2 B10", "0:150 1:150", "A:801009311;C:582803669;G:1691856038;T:743070146;N:16936", 150, 150, null, null, 801009311, 582803669, 1691856038, 743070146, 16936, "ERX14341144", "ERS24422761", "ERA33115582", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "T", "T", "mates < 9% mapping rate", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19251, "ERR14935145", "ERX14339387", "ERS24360102", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well H09", "Humanized Plate 1   Well H09", "SAMEA118225025", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample HUM1 H09|collection date:2022 11 14|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample HUM1 H09|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample HUM1 H09", "webin reads Sample HUM1 H09", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample HUM1 H09", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "H09.R1.fastq.gz H09.R2.fastq.gz", "fastq fastq", 79435500.0, 264785.0, "webin reads Sample HUM1 H09", "0:150 1:150", "A:19325284;C:9616771;G:32084598;T:18408162;N:685", 150, 150, null, null, 19325284, 9616771, 32084598, 18408162, 685, "ERX14339387", "ERS24360102", "ERA33113089", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "T", "T", "mates < 9% mapping rate", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19252, "ERR14935006", "ERX14339248", "ERS24360059", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well E02", "Humanized Plate 1   Well E02", "SAMEA118224982", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample HUM1 E02|collection date:2022 11 14|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample HUM1 E02|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample HUM1 E02", "webin reads Sample HUM1 E02", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample HUM1 E02", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "E02.R1.fastq.gz E02.R2.fastq.gz", "fastq fastq", 184473600.0, 614912.0, "webin reads Sample HUM1 E02", "0:150 1:150", "A:47275758;C:21941025;G:68635035;T:46620684;N:1098", 150, 150, null, null, 47275758, 21941025, 68635035, 46620684, 1098, "ERX14339248", "ERS24360059", "ERA33112606", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "T", "T", "mates < 9% mapping rate", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19253, "ERR14937114", "ERX14341358", "ERS24422807", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well F08", "Humanized Plate 2   Well F08", "SAMEA118242633", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample HUM2 F08|collection date:2022 12 21|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample HUM2 F08|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample HUM2 F08", "webin reads Sample HUM2 F08", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample HUM2 F08", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "F08.R1.fastq.gz F08.R2.fastq.gz", "fastq fastq", 5604971400.0, 18683238.0, "webin reads Sample HUM2 F08", "0:150 1:150", "A:1272096391;C:927796029;G:2253030240;T:1152025287;N:23453", 150, 150, null, null, 1272096391, 927796029, 2253030240, 1152025287, 23453, "ERX14341358", "ERS24422807", "ERA33115697", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "T", "T", "mates < 9% mapping rate", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19254, "ERR14935115", "ERX14339357", "ERS24360092", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well G11", "Humanized Plate 1   Well G11", "SAMEA118225015", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample HUM1 G11|collection date:2022 11 14|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample HUM1 G11|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample HUM1 G11", "webin reads Sample HUM1 G11", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample HUM1 G11", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "G11.R1.fastq.gz G11.R2.fastq.gz", "fastq fastq", 18225600.0, 60752.0, "webin reads Sample HUM1 G11", "0:150 1:150", "A:3396385;C:2248165;G:9171340;T:3409593;N:117", 150, 150, null, null, 3396385, 2248165, 9171340, 3409593, 117, "ERX14339357", "ERS24360092", "ERA33112973", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "T", "T", "mates < 9% mapping rate", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19255, "ERR14950748", "ERX14354872", "ERS24422916", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well F01", "Knockout Plate   Well F01", "SAMEA118242742", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample KO F01|collection date:2022 05 13|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample KO F01|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample KO F01", "webin reads Sample KO F01", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample KO F01", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "F01.R1.fastq.gz F01.R2.fastq.gz", "fastq fastq", 3161931900.0, 10539773.0, "webin reads Sample KO F01", "0:150 1:150", "A:1111325789;C:460593692;G:495389420;T:1094598021;N:24978", 150, 150, null, null, 1111325789, 460593692, 495389420, 1094598021, 24978, "ERX14354872", "ERS24422916", "ERA33121243", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "T", "B", "mate1 technical by mapping diff", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19256, "ERR14934881", "ERX14339123", "ERS24360033", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well B12", "Humanized Plate 1   Well B12", "SAMEA118224956", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample HUM1 B12|collection date:2022 11 14|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample HUM1 B12|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample HUM1 B12", "webin reads Sample HUM1 B12", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample HUM1 B12", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "B12.R1.fastq.gz B12.R2.fastq.gz", "fastq fastq", 116537700.0, 388459.0, "webin reads Sample HUM1 B12", "0:150 1:150", "A:27658956;C:13844738;G:48483886;T:26549255;N:865", 150, 150, null, null, 27658956, 13844738, 48483886, 26549255, 865, "ERX14339123", "ERS24360033", "ERA33112224", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "T", "T", "mates < 9% mapping rate", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19257, "ERR14934824", "ERX14339066", "ERS24360013", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well A04", "Humanized Plate 1   Well A04", "SAMEA118224936", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample HUM1 A04|collection date:2022 11 14|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample HUM1 A04|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample HUM1 A04", "webin reads Sample HUM1 A04", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample HUM1 A04", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "A04.R1.fastq.gz A04.R2.fastq.gz", "fastq fastq", 190861200.0, 636204.0, "webin reads Sample HUM1 A04", "0:150 1:150", "A:51866429;C:22469460;G:65825429;T:50698008;N:1874", 150, 150, null, null, 51866429, 22469460, 65825429, 50698008, 1874, "ERX14339066", "ERS24360013", "ERA33112023", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19258, "ERR14934950", "ERX14339192", "ERS24360041", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well C08", "Humanized Plate 1   Well C08", "SAMEA118224964", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample HUM1 C08|collection date:2022 11 14|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample HUM1 C08|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample HUM1 C08", "webin reads Sample HUM1 C08", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample HUM1 C08", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "C08.R1.fastq.gz C08.R2.fastq.gz", "fastq fastq", 347655600.0, 1158852.0, "webin reads Sample HUM1 C08", "0:150 1:150", "A:111273717;C:41410769;G:81188146;T:113779846;N:3122", 150, 150, null, null, 111273717, 41410769, 81188146, 113779846, 3122, "ERX14339192", "ERS24360041", "ERA33112408", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "mate1-mate2 similar by mapping diff", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19259, "ERR14936959", "ERX14341201", "ERS24422772", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well C09", "Humanized Plate 2   Well C09", "SAMEA118242598", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample HUM2 C09|collection date:2022 12 21|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample HUM2 C09|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample HUM2 C09", "webin reads Sample HUM2 C09", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample HUM2 C09", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "C09.R1.fastq.gz C09.R2.fastq.gz", "fastq fastq", 3716150400.0, 12387168.0, "webin reads Sample HUM2 C09", "0:150 1:150", "A:950093356;C:589554852;G:1341746189;T:834739539;N:16464", 150, 150, null, null, 950093356, 589554852, 1341746189, 834739539, 16464, "ERX14341201", "ERS24422772", "ERA33115600", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "T", "T", "mates < 9% mapping rate", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19260, "ERR14934954", "ERX14339196", "ERS24360042", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well C09", "Humanized Plate 1   Well C09", "SAMEA118224965", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample HUM1 C09|collection date:2022 11 14|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample HUM1 C09|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample HUM1 C09", "webin reads Sample HUM1 C09", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample HUM1 C09", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "C09.R1.fastq.gz C09.R2.fastq.gz", "fastq fastq", 160752900.0, 535843.0, "webin reads Sample HUM1 C09", "0:150 1:150", "A:47408978;C:19223052;G:47019288;T:47100126;N:1456", 150, 150, null, null, 47408978, 19223052, 47019288, 47100126, 1456, "ERX14339196", "ERS24360042", "ERA33112423", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "mate1-mate2 similar by mapping diff", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19261, "ERR14950615", "ERX14354739", "ERS24422889", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well C01", "Knockout Plate   Well C01", "SAMEA118242715", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample KO C01|collection date:2022 05 13|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample KO C01|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample KO C01", "webin reads Sample KO C01", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample KO C01", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "C01.R1.fastq.gz C01.R2.fastq.gz", "fastq fastq", 12926966100.0, 43089887.0, "webin reads Sample KO C01", "0:150 1:150", "A:4670066457;C:1792574588;G:2068594468;T:4395628669;N:101918", 150, 150, null, null, 4670066457, 1792574588, 2068594468, 4395628669, 101918, "ERX14354739", "ERS24422889", "ERA33121110", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19262, "ERR14950602", "ERX14354726", "ERS24422887", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well B11", "Knockout Plate   Well B11", "SAMEA118242713", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample KO B11|collection date:2022 05 13|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample KO B11|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample KO B11", "webin reads Sample KO B11", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample KO B11", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "B11.R1.fastq.gz B11.R2.fastq.gz", "fastq fastq", 7240824000.0, 24136080.0, "webin reads Sample KO B11", "0:150 1:150", "A:2670219676;C:931192972;G:1216106261;T:2423246169;N:58922", 150, 150, null, null, 2670219676, 931192972, 1216106261, 2423246169, 58922, "ERX14354726", "ERS24422887", "ERA33121097", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19263, "ERR14950677", "ERX14354801", "ERS24422900", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well C12", "Knockout Plate   Well C12", "SAMEA118242726", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample KO C12|collection date:2022 05 13|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample KO C12|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample KO C12", "webin reads Sample KO C12", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample KO C12", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "C12.R1.fastq.gz C12.R2.fastq.gz", "fastq fastq", 8490801000.0, 28302670.0, "webin reads Sample KO C12", "0:150 1:150", "A:3049316720;C:1192674579;G:1458160432;T:2790581120;N:68149", 150, 150, null, null, 3049316720, 1192674579, 1458160432, 2790581120, 68149, "ERX14354801", "ERS24422900", "ERA33121172", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19264, "ERR14950597", "ERX14354721", "ERS24422886", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well B10", "Knockout Plate   Well B10", "SAMEA118242712", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample KO B10|collection date:2022 05 13|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample KO B10|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample KO B10", "webin reads Sample KO B10", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample KO B10", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "B10.R1.fastq.gz B10.R2.fastq.gz", "fastq fastq", 9344290800.0, 31147636.0, "webin reads Sample KO B10", "0:150 1:150", "A:3401103081;C:1284404735;G:1572646132;T:3086062190;N:74662", 150, 150, null, null, 3401103081, 1284404735, 1572646132, 3086062190, 74662, "ERX14354721", "ERS24422886", "ERA33121092", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19265, "ERR14950734", "ERX14354858", "ERS24422911", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well E08", "Knockout Plate   Well E08", "SAMEA118242737", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample KO E08|collection date:2022 05 13|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample KO E08|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample KO E08", "webin reads Sample KO E08", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample KO E08", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "E08.R1.fastq.gz E08.R2.fastq.gz", "fastq fastq", 1843996200.0, 6146654.0, "webin reads Sample KO E08", "0:150 1:150", "A:639221474;C:243164058;G:390761341;T:570834581;N:14746", 150, 150, null, null, 639221474, 243164058, 390761341, 570834581, 14746, "ERX14354858", "ERS24422911", "ERA33121229", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "mate1-mate2 similar by mapping diff", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19266, "ERR14943982", "ERX14348134", "ERS24422877", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well B01", "Knockout Plate   Well B01", "SAMEA118242703", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample KO B01|collection date:2022 05 13|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample KO B01|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample KO B01", "webin reads Sample KO B01", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample KO B01", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "B01.R1.fastq.gz B01.R2.fastq.gz", "fastq fastq", 11318469900.0, 37728233.0, "webin reads Sample KO B01", "0:150 1:150", "A:4086308197;C:1546883485;G:1844189302;T:3841000200;N:88716", 150, 150, null, null, 4086308197, 1546883485, 1844189302, 3841000200, 88716, "ERX14348134", "ERS24422877", "ERA33119688", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19267, "ERR14950636", "ERX14354760", "ERS24422893", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well C05", "Knockout Plate   Well C05", "SAMEA118242719", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample KO C05|collection date:2022 05 13|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample KO C05|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample KO C05", "webin reads Sample KO C05", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample KO C05", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "C05.R2.fastq.gz C05.R1.fastq.gz", "fastq fastq", 13861467300.0, 46204891.0, "webin reads Sample KO C05", "0:150 1:150", "A:4748497970;C:2174952613;G:2309004592;T:4628898110;N:114015", 150, 150, null, null, 4748497970, 2174952613, 2309004592, 4628898110, 114015, "ERX14354760", "ERS24422893", "ERA33121131", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19268, "ERR14935064", "ERX14339306", "ERS24360078", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well F09", "Humanized Plate 1   Well F09", "SAMEA118225001", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample HUM1 F09|collection date:2022 11 14|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample HUM1 F09|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample HUM1 F09", "webin reads Sample HUM1 F09", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample HUM1 F09", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "F09.R1.fastq.gz F09.R2.fastq.gz", "fastq fastq", 393873600.0, 1312912.0, "webin reads Sample HUM1 F09", "0:150 1:150", "A:125159366;C:49326024;G:94522011;T:124863337;N:2862", 150, 150, null, null, 125159366, 49326024, 94522011, 124863337, 2862, "ERX14339306", "ERS24360078", "ERA33112801", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19269, "ERR14934858", "ERX14339100", "ERS24360025", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well B04", "Humanized Plate 1   Well B04", "SAMEA118224948", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample HUM1 B04|collection date:2022 11 14|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample HUM1 B04|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample HUM1 B04", "webin reads Sample HUM1 B04", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample HUM1 B04", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "B04.R1.fastq.gz B04.R2.fastq.gz", "fastq fastq", 85698600.0, 285662.0, "webin reads Sample HUM1 B04", "0:150 1:150", "A:16127871;C:10936313;G:42563818;T:16070081;N:517", 150, 150, null, null, 16127871, 10936313, 42563818, 16070081, 517, "ERX14339100", "ERS24360025", "ERA33112146", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "T", "T", "mates < 9% mapping rate", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19270, "ERR14950548", "ERX14354672", "ERS24422866", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well A02", "Knockout Plate   Well A02", "SAMEA118242692", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample KO A02|collection date:2022 05 13|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample KO A02|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample KO A02", "webin reads Sample KO A02", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample KO A02", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "A02.R1.fastq.gz A02.R2.fastq.gz", "fastq fastq", 3357020700.0, 11190069.0, "webin reads Sample KO A02", "0:150 1:150", "A:1198525901;C:478732184;G:572930834;T:1106804630;N:27151", 150, 150, null, null, 1198525901, 478732184, 572930834, 1106804630, 27151, "ERX14354672", "ERS24422866", "ERA33121043", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19271, "ERR14936843", "ERX14341085", "ERS24422751", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well A12", "Humanized Plate 2   Well A12", "SAMEA118242577", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample HUM2 A12|collection date:2022 12 21|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample HUM2 A12|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample HUM2 A12", "webin reads Sample HUM2 A12", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample HUM2 A12", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "A12.R1.fastq.gz A12.R2.fastq.gz", "fastq fastq", 10256706300.0, 34189021.0, "webin reads Sample HUM2 A12", "0:150 1:150", "A:3536584478;C:1403340948;G:2397966213;T:2918763136;N:51525", 150, 150, null, null, 3536584478, 1403340948, 2397966213, 2918763136, 51525, "ERX14341085", "ERS24422751", "ERA33115534", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "mate1-mate2 similar by mapping diff", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19272, "ERR14950795", "ERX14354919", "ERS24422930", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well G03", "Knockout Plate   Well G03", "SAMEA118242756", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample KO G03|collection date:2022 05 13|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample KO G03|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample KO G03", "webin reads Sample KO G03", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample KO G03", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "G03.R1.fastq.gz G03.R2.fastq.gz", "fastq fastq", 7364946000.0, 24549820.0, "webin reads Sample KO G03", "0:150 1:150", "A:2672614853;C:1036322394;G:1247472746;T:2408476880;N:59127", 150, 150, null, null, 2672614853, 1036322394, 1247472746, 2408476880, 59127, "ERX14354919", "ERS24422930", "ERA33121291", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19273, "ERR14935029", "ERX14339271", "ERS24360067", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well E10", "Humanized Plate 1   Well E10", "SAMEA118224990", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample HUM1 E10|collection date:2022 11 14|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample HUM1 E10|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample HUM1 E10", "webin reads Sample HUM1 E10", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample HUM1 E10", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "E10.R1.fastq.gz E10.R2.fastq.gz", "fastq fastq", 137180100.0, 457267.0, "webin reads Sample HUM1 E10", "0:150 1:150", "A:33764068;C:16452119;G:53129173;T:33833524;N:1216", 150, 150, null, null, 33764068, 16452119, 53129173, 33833524, 1216, "ERX14339271", "ERS24360067", "ERA33112682", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "T", "T", "mates < 9% mapping rate", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19274, "ERR14937113", "ERX14341357", "ERS24422806", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well F07", "Humanized Plate 2   Well F07", "SAMEA118242632", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample HUM2 F07|collection date:2022 12 21|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample HUM2 F07|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample HUM2 F07", "webin reads Sample HUM2 F07", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample HUM2 F07", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "F07.R1.fastq.gz F07.R2.fastq.gz", "fastq fastq", 3764933100.0, 12549777.0, "webin reads Sample HUM2 F07", "0:150 1:150", "A:931279746;C:549119528;G:1391605324;T:892911306;N:17196", 150, 150, null, null, 931279746, 549119528, 1391605324, 892911306, 17196, "ERX14341357", "ERS24422806", "ERA33115695", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "T", "T", "mates < 9% mapping rate", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19275, "ERR14952973", "ERX14357127", "ERS24422835", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well H12", "Humanized Plate 2   Well H12", "SAMEA118242661", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample HUM2 H12|collection date:2022 12 21|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample HUM2 H12|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample HUM2 H12", "webin reads Sample HUM2 H12", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample HUM2 H12", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "H12.R1.fastq.gz H12.R2.fastq.gz", "fastq fastq", 4110320400.0, 13701068.0, "webin reads Sample HUM2 H12", "0:150 1:150", "A:994038023;C:659909232;G:1578428805;T:877925810;N:18530", 150, 150, null, null, 994038023, 659909232, 1578428805, 877925810, 18530, "ERX14357127", "ERS24422835", "ERA33122913", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "T", "T", "mates < 9% mapping rate", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19276, "ERR14935019", "ERX14339261", "ERS24360063", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well E06", "Humanized Plate 1   Well E06", "SAMEA118224986", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample HUM1 E06|collection date:2022 11 14|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample HUM1 E06|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample HUM1 E06", "webin reads Sample HUM1 E06", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample HUM1 E06", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "E06.R1.fastq.gz E06.R2.fastq.gz", "fastq fastq", 250613700.0, 835379.0, "webin reads Sample HUM1 E06", "0:150 1:150", "A:75679048;C:30156356;G:70614639;T:74161729;N:1928", 150, 150, null, null, 75679048, 30156356, 70614639, 74161729, 1928, "ERX14339261", "ERS24360063", "ERA33112650", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19277, "ERR14937144", "ERX14341388", "ERS24422814", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well G03", "Humanized Plate 2   Well G03", "SAMEA118242640", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample HUM2 G03|collection date:2022 12 21|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample HUM2 G03|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample HUM2 G03", "webin reads Sample HUM2 G03", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample HUM2 G03", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "G03.R1.fastq.gz G03.R2.fastq.gz", "fastq fastq", 4967196600.0, 16557322.0, "webin reads Sample HUM2 G03", "0:150 1:150", "A:1310441558;C:730142812;G:1820047798;T:1106541425;N:23007", 150, 150, null, null, 1310441558, 730142812, 1820047798, 1106541425, 23007, "ERX14341388", "ERS24422814", "ERA33115713", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "T", "T", "mates < 9% mapping rate", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19278, "ERR14936818", "ERX14341060", "ERS24422741", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well A02", "Humanized Plate 2   Well A02", "SAMEA118242567", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample HUM2 A02|collection date:2022 12 21|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample HUM2 A02|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample HUM2 A02", "webin reads Sample HUM2 A02", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample HUM2 A02", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "A02.R1.fastq.gz A02.R2.fastq.gz", "fastq fastq", 3217733700.0, 10725779.0, "webin reads Sample HUM2 A02", "0:150 1:150", "A:686575330;C:510490874;G:1381211600;T:639440992;N:14904", 150, 150, null, null, 686575330, 510490874, 1381211600, 639440992, 14904, "ERX14341060", "ERS24422741", "ERA33115505", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "T", "T", "mates < 9% mapping rate", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19279, "ERR14950774", "ERX14354898", "ERS24422922", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well F07", "Knockout Plate   Well F07", "SAMEA118242748", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample KO F07|collection date:2022 05 13|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample KO F07|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample KO F07", "webin reads Sample KO F07", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample KO F07", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "F07.R1.fastq.gz F07.R2.fastq.gz", "fastq fastq", 10763253300.0, 35877511.0, "webin reads Sample KO F07", "0:150 1:150", "A:3904704604;C:1492936538;G:1705278852;T:3660244628;N:88678", 150, 150, null, null, 3904704604, 1492936538, 1705278852, 3660244628, 88678, "ERX14354898", "ERS24422922", "ERA33121270", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19280, "ERR14934968", "ERX14339210", "ERS24360046", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well D01", "Humanized Plate 1   Well D01", "SAMEA118224969", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample HUM1 D01|collection date:2022 11 14|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample HUM1 D01|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample HUM1 D01", "webin reads Sample HUM1 D01", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample HUM1 D01", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "D01.R1.fastq.gz D01.R2.fastq.gz", "fastq fastq", 169613700.0, 565379.0, "webin reads Sample HUM1 D01", "0:150 1:150", "A:44186025;C:22437297;G:59635089;T:43353786;N:1503", 150, 150, null, null, 44186025, 22437297, 59635089, 43353786, 1503, "ERX14339210", "ERS24360046", "ERA33112474", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "T", "T", "mates < 9% mapping rate", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19281, "ERR14944006", "ERX14348158", "ERS24422884", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well B08", "Knockout Plate   Well B08", "SAMEA118242710", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample KO B08|collection date:2022 05 13|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample KO B08|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample KO B08", "webin reads Sample KO B08", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample KO B08", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "B08.R1.fastq.gz B08.R2.fastq.gz", "fastq fastq", 4374918300.0, 14583061.0, "webin reads Sample KO B08", "0:150 1:150", "A:1502989289;C:608306022;G:868547831;T:1395039881;N:35277", 150, 150, null, null, 1502989289, 608306022, 868547831, 1395039881, 35277, "ERX14348158", "ERS24422884", "ERA33119742", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19282, "ERR14935068", "ERX14339310", "ERS24360079", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well F10", "Humanized Plate 1   Well F10", "SAMEA118225002", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample HUM1 F10|collection date:2022 11 14|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample HUM1 F10|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample HUM1 F10", "webin reads Sample HUM1 F10", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample HUM1 F10", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "F10.R1.fastq.gz F10.R2.fastq.gz", "fastq fastq", 366521100.0, 1221737.0, "webin reads Sample HUM1 F10", "0:150 1:150", "A:120362548;C:43174281;G:81935740;T:121045446;N:3085", 150, 150, null, null, 120362548, 43174281, 81935740, 121045446, 3085, "ERX14339310", "ERS24360079", "ERA33112816", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "mate1-mate2 similar by mapping diff", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19283, "ERR14935151", "ERX14339393", "ERS24360105", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well H12", "Humanized Plate 1   Well H12", "SAMEA118225028", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample HUM1 H12|collection date:2022 11 14|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample HUM1 H12|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample HUM1 H12", "webin reads Sample HUM1 H12", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample HUM1 H12", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "H12.R1.fastq.gz H12.R2.fastq.gz", "fastq fastq", 64716600.0, 215722.0, "webin reads Sample HUM1 H12", "0:150 1:150", "A:11919103;C:8780603;G:32437007;T:11579359;N:528", 150, 150, null, null, 11919103, 8780603, 32437007, 11579359, 528, "ERX14339393", "ERS24360105", "ERA33113116", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "T", "T", "mates < 9% mapping rate", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19284, "ERR14950775", "ERX14354899", "ERS24422923", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well F08", "Knockout Plate   Well F08", "SAMEA118242749", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample KO F08|collection date:2022 05 13|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample KO F08|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample KO F08", "webin reads Sample KO F08", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample KO F08", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "F08.R1.fastq.gz F08.R2.fastq.gz", "fastq fastq", 862013100.0, 2873377.0, "webin reads Sample KO F08", "0:150 1:150", "A:258529164;C:123157615;G:260317379;T:220001971;N:6971", 150, 150, null, null, 258529164, 123157615, 260317379, 220001971, 6971, "ERX14354899", "ERS24422923", "ERA33121271", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "T", "B", "mate1 technical by mapping diff", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19285, "ERR14934987", "ERX14339229", "ERS24360050", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well D05", "Humanized Plate 1   Well D05", "SAMEA118224973", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample HUM1 D05|collection date:2022 11 14|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample HUM1 D05|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample HUM1 D05", "webin reads Sample HUM1 D05", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample HUM1 D05", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "D05.R1.fastq.gz D05.R2.fastq.gz", "fastq fastq", 87887400.0, 292958.0, "webin reads Sample HUM1 D05", "0:150 1:150", "A:19996165;C:11416204;G:37562812;T:18911751;N:468", 150, 150, null, null, 19996165, 11416204, 37562812, 18911751, 468, "ERX14339229", "ERS24360050", "ERA33112543", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "T", "T", "mates < 9% mapping rate", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19286, "ERR14950668", "ERX14354792", "ERS24422898", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well C10", "Knockout Plate   Well C10", "SAMEA118242724", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample KO C10|collection date:2022 05 13|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample KO C10|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample KO C10", "webin reads Sample KO C10", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample KO C10", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "C10.R1.fastq.gz C10.R2.fastq.gz", "fastq fastq", 2650018500.0, 8833395.0, "webin reads Sample KO C10", "0:150 1:150", "A:944147055;C:380161906;G:445871150;T:879817280;N:21109", 150, 150, null, null, 944147055, 380161906, 445871150, 879817280, 21109, "ERX14354792", "ERS24422898", "ERA33121163", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "mate1-mate2 similar by mapping diff", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19287, "ERR14937108", "ERX14341352", "ERS24422801", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well F02", "Humanized Plate 2   Well F02", "SAMEA118242627", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample HUM2 F02|collection date:2022 12 21|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample HUM2 F02|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample HUM2 F02", "webin reads Sample HUM2 F02", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample HUM2 F02", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "F02.R1.fastq.gz F02.R2.fastq.gz", "fastq fastq", 4319451600.0, 14398172.0, "webin reads Sample HUM2 F02", "0:150 1:150", "A:1013913720;C:720345672;G:1637113325;T:948057846;N:21037", 150, 150, null, null, 1013913720, 720345672, 1637113325, 948057846, 21037, "ERX14341352", "ERS24422801", "ERA33115690", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "T", "T", "mates < 9% mapping rate", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19288, "ERR14934840", "ERX14339082", "ERS24360019", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well A10", "Humanized Plate 1   Well A10", "SAMEA118224942", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample HUM1 A10|collection date:2022 11 14|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample HUM1 A10|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample HUM1 A10", "webin reads Sample HUM1 A10", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample HUM1 A10", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "A10.R1.fastq.gz A10.R2.fastq.gz", "fastq fastq", 55647900.0, 185493.0, "webin reads Sample HUM1 A10", "0:150 1:150", "A:8450799;C:7127607;G:31284165;T:8784914;N:415", 150, 150, null, null, 8450799, 7127607, 31284165, 8784914, 415, "ERX14339082", "ERS24360019", "ERA33112084", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "T", "T", "mates < 9% mapping rate", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19289, "ERR14935132", "ERX14339374", "ERS24360098", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well H05", "Humanized Plate 1   Well H05", "SAMEA118225021", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample HUM1 H05|collection date:2022 11 14|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample HUM1 H05|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample HUM1 H05", "webin reads Sample HUM1 H05", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample HUM1 H05", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "H05.R1.fastq.gz H05.R2.fastq.gz", "fastq fastq", 161532600.0, 538442.0, "webin reads Sample HUM1 H05", "0:150 1:150", "A:46954082;C:18456156;G:50213476;T:45907368;N:1518", 150, 150, null, null, 46954082, 18456156, 50213476, 45907368, 1518, "ERX14339374", "ERS24360098", "ERA33113034", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "mate1-mate2 similar by mapping diff", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19290, "ERR14934993", "ERX14339235", "ERS24360053", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well D08", "Humanized Plate 1   Well D08", "SAMEA118224976", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample HUM1 D08|collection date:2022 11 14|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample HUM1 D08|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample HUM1 D08", "webin reads Sample HUM1 D08", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample HUM1 D08", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "D08.R1.fastq.gz D08.R2.fastq.gz", "fastq fastq", 163574400.0, 545248.0, "webin reads Sample HUM1 D08", "0:150 1:150", "A:36914350;C:20424845;G:69296209;T:36937661;N:1335", 150, 150, null, null, 36914350, 20424845, 69296209, 36937661, 1335, "ERX14339235", "ERS24360053", "ERA33112562", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "T", "T", "mates < 9% mapping rate", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19291, "ERR14934860", "ERX14339102", "ERS24360026", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well B05", "Humanized Plate 1   Well B05", "SAMEA118224949", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample HUM1 B05|collection date:2022 11 14|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample HUM1 B05|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample HUM1 B05", "webin reads Sample HUM1 B05", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample HUM1 B05", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "B05.R1.fastq.gz B05.R2.fastq.gz", "fastq fastq", 104906100.0, 349687.0, "webin reads Sample HUM1 B05", "0:150 1:150", "A:27195212;C:13543121;G:38418859;T:25748246;N:662", 150, 150, null, null, 27195212, 13543121, 38418859, 25748246, 662, "ERX14339102", "ERS24360026", "ERA33112153", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "T", "T", "mates < 9% mapping rate", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19292, "ERR14937153", "ERX14341397", "ERS24422823", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well G12", "Humanized Plate 2   Well G12", "SAMEA118242649", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample HUM2 G12|collection date:2022 12 21|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample HUM2 G12|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample HUM2 G12", "webin reads Sample HUM2 G12", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample HUM2 G12", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "G12.R1.fastq.gz G12.R2.fastq.gz", "fastq fastq", 4617277800.0, 15390926.0, "webin reads Sample HUM2 G12", "0:150 1:150", "A:1158729179;C:728158561;G:1691881391;T:1038487859;N:20810", 150, 150, null, null, 1158729179, 728158561, 1691881391, 1038487859, 20810, "ERX14341397", "ERS24422823", "ERA33115725", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "T", "T", "mates < 9% mapping rate", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19293, "ERR14943976", "ERX14348128", "ERS24422873", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well A09", "Knockout Plate   Well A09", "SAMEA118242699", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample KO A09|collection date:2022 05 13|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample KO A09|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample KO A09", "webin reads Sample KO A09", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample KO A09", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "A09.R1.fastq.gz A09.R2.fastq.gz", "fastq fastq", 7026339600.0, 23421132.0, "webin reads Sample KO A09", "0:150 1:150", "A:2584462615;C:932829225;G:1126129540;T:2382863475;N:54745", 150, 150, null, null, 2584462615, 932829225, 1126129540, 2382863475, 54745, "ERX14348128", "ERS24422873", "ERA33119655", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19294, "ERR14934848", "ERX14339090", "ERS24360022", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well B01", "Humanized Plate 1   Well B01", "SAMEA118224945", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample HUM1 B01|collection date:2022 11 14|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample HUM1 B01|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample HUM1 B01", "webin reads Sample HUM1 B01", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample HUM1 B01", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "B01.R1.fastq.gz B01.R2.fastq.gz", "fastq fastq", 75625800.0, 252086.0, "webin reads Sample HUM1 B01", "0:150 1:150", "A:11997345;C:9943452;G:40709427;T:12974865;N:711", 150, 150, null, null, 11997345, 9943452, 40709427, 12974865, 711, "ERX14339090", "ERS24360022", "ERA33112111", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "T", "T", "mates < 9% mapping rate", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19295, "ERR14934832", "ERX14339074", "ERS24360016", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well A07", "Humanized Plate 1   Well A07", "SAMEA118224939", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample HUM1 A07|collection date:2022 11 14|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample HUM1 A07|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample HUM1 A07", "webin reads Sample HUM1 A07", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample HUM1 A07", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "A07.R1.fastq.gz A07.R2.fastq.gz", "fastq fastq", 69187800.0, 230626.0, "webin reads Sample HUM1 A07", "0:150 1:150", "A:12381283;C:7906319;G:37465324;T:11434563;N:311", 150, 150, null, null, 12381283, 7906319, 37465324, 11434563, 311, "ERX14339074", "ERS24360016", "ERA33112056", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "T", "T", "mates < 9% mapping rate", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19296, "ERR14935010", "ERX14339252", "ERS24360060", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well E03", "Humanized Plate 1   Well E03", "SAMEA118224983", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample HUM1 E03|collection date:2022 11 14|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample HUM1 E03|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample HUM1 E03", "webin reads Sample HUM1 E03", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample HUM1 E03", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "E03.R1.fastq.gz E03.R2.fastq.gz", "fastq fastq", 425713200.0, 1419044.0, "webin reads Sample HUM1 E03", "0:150 1:150", "A:136345366;C:51139029;G:106693807;T:131531022;N:3976", 150, 150, null, null, 136345366, 51139029, 106693807, 131531022, 3976, "ERX14339252", "ERS24360060", "ERA33112619", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19297, "ERR14937160", "ERX14341404", "ERS24422830", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well H07", "Humanized Plate 2   Well H07", "SAMEA118242656", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample HUM2 H07|collection date:2022 12 21|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample HUM2 H07|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample HUM2 H07", "webin reads Sample HUM2 H07", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample HUM2 H07", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "H07.R1.fastq.gz H07.R2.fastq.gz", "fastq fastq", 2014913100.0, 6716377.0, "webin reads Sample HUM2 H07", "0:150 1:150", "A:411170361;C:304335223;G:896421902;T:402976937;N:8677", 150, 150, null, null, 411170361, 304335223, 896421902, 402976937, 8677, "ERX14341404", "ERS24422830", "ERA33115735", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "T", "T", "mates < 9% mapping rate", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19298, "ERR14936962", "ERX14341204", "ERS24422793", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well E06", "Humanized Plate 2   Well E06", "SAMEA118242619", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample HUM2 E06|collection date:2022 12 21|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample HUM2 E06|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample HUM2 E06", "webin reads Sample HUM2 E06", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample HUM2 E06", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "E06.R1.fastq.gz E06.R2.fastq.gz", "fastq fastq", 10133040000.0, 33776800.0, "webin reads Sample HUM2 E06", "0:150 1:150", "A:3260003556;C:1412856784;G:2700597066;T:2759534229;N:48365", 150, 150, null, null, 3260003556, 1412856784, 2700597066, 2759534229, 48365, "ERX14341204", "ERS24422793", "ERA33115605", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "T", "T", "mates < 9% mapping rate", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19299, "ERR14950764", "ERX14354888", "ERS24422920", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well F05", "Knockout Plate   Well F05", "SAMEA118242746", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample KO F05|collection date:2022 05 13|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample KO F05|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample KO F05", "webin reads Sample KO F05", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample KO F05", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "F05.R1.fastq.gz F05.R2.fastq.gz", "fastq fastq", 6748159500.0, 22493865.0, "webin reads Sample KO F05", "0:150 1:150", "A:2431257026;C:944096584;G:1125684524;T:2247068351;N:53015", 150, 150, null, null, 2431257026, 944096584, 1125684524, 2247068351, 53015, "ERX14354888", "ERS24422920", "ERA33121260", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19300, "ERR14936821", "ERX14341063", "ERS24422744", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well A05", "Humanized Plate 2   Well A05", "SAMEA118242570", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample HUM2 A05|collection date:2022 12 21|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample HUM2 A05|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample HUM2 A05", "webin reads Sample HUM2 A05", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample HUM2 A05", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "A05.R1.fastq.gz A05.R2.fastq.gz", "fastq fastq", 3531016500.0, 11770055.0, "webin reads Sample HUM2 A05", "0:150 1:150", "A:908506275;C:540353948;G:1299418302;T:782721784;N:16191", 150, 150, null, null, 908506275, 540353948, 1299418302, 782721784, 16191, "ERX14341063", "ERS24422744", "ERA33115508", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "T", "T", "mates < 9% mapping rate", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19301, "ERR14950698", "ERX14354822", "ERS24422903", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well D03", "Knockout Plate   Well D03", "SAMEA118242729", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample KO D03|collection date:2022 05 13|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample KO D03|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample KO D03", "webin reads Sample KO D03", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample KO D03", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "D03.R1.fastq.gz D03.R2.fastq.gz", "fastq fastq", 15044100900.0, 50147003.0, "webin reads Sample KO D03", "0:150 1:150", "A:5448629271;C:2142046198;G:2372663075;T:5080644849;N:117507", 150, 150, null, null, 5448629271, 2142046198, 2372663075, 5080644849, 117507, "ERX14354822", "ERS24422903", "ERA33121193", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19302, "ERR14950732", "ERX14354856", "ERS24422910", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well E07", "Knockout Plate   Well E07", "SAMEA118242736", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample KO E07|collection date:2022 05 13|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample KO E07|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample KO E07", "webin reads Sample KO E07", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample KO E07", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "E07.R1.fastq.gz E07.R2.fastq.gz", "fastq fastq", 12898175700.0, 42993919.0, "webin reads Sample KO E07", "0:150 1:150", "A:4589735008;C:1839814686;G:2098030544;T:4370493808;N:101654", 150, 150, null, null, 4589735008, 1839814686, 2098030544, 4370493808, 101654, "ERX14354856", "ERS24422910", "ERA33121227", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19303, "ERR14934842", "ERX14339084", "ERS24360020", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well A11", "Humanized Plate 1   Well A11", "SAMEA118224943", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample HUM1 A11|collection date:2022 11 14|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample HUM1 A11|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample HUM1 A11", "webin reads Sample HUM1 A11", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample HUM1 A11", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "A11.R1.fastq.gz A11.R2.fastq.gz", "fastq fastq", 53858400.0, 179528.0, "webin reads Sample HUM1 A11", "0:150 1:150", "A:12307549;C:6368678;G:23753869;T:11428030;N:274", 150, 150, null, null, 12307549, 6368678, 23753869, 11428030, 274, "ERX14339084", "ERS24360020", "ERA33112092", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "T", "T", "mates < 9% mapping rate", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19304, "ERR14950672", "ERX14354796", "ERS24422899", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well C11", "Knockout Plate   Well C11", "SAMEA118242725", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample KO C11|collection date:2022 05 13|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample KO C11|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample KO C11", "webin reads Sample KO C11", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample KO C11", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "C11.R1.fastq.gz C11.R2.fastq.gz", "fastq fastq", 6484306200.0, 21614354.0, "webin reads Sample KO C11", "0:150 1:150", "A:2345044923;C:858634350;G:1122803400;T:2157772266;N:51261", 150, 150, null, null, 2345044923, 858634350, 1122803400, 2157772266, 51261, "ERX14354796", "ERS24422899", "ERA33121167", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19305, "ERR14934989", "ERX14339231", "ERS24360051", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well D06", "Humanized Plate 1   Well D06", "SAMEA118224974", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample HUM1 D06|collection date:2022 11 14|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample HUM1 D06|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample HUM1 D06", "webin reads Sample HUM1 D06", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample HUM1 D06", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "D06.R1.fastq.gz D06.R2.fastq.gz", "fastq fastq", 110618100.0, 368727.0, "webin reads Sample HUM1 D06", "0:150 1:150", "A:26616860;C:14643839;G:43583750;T:25772923;N:728", 150, 150, null, null, 26616860, 14643839, 43583750, 25772923, 728, "ERX14339231", "ERS24360051", "ERA33112549", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "T", "T", "mates < 9% mapping rate", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19306, "ERR14950801", "ERX14354925", "ERS24422933", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well G06", "Knockout Plate   Well G06", "SAMEA118242759", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample KO G06|collection date:2022 05 13|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample KO G06|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample KO G06", "webin reads Sample KO G06", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample KO G06", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "G06.R1.fastq.gz G06.R2.fastq.gz", "fastq fastq", 1620231000.0, 5400770.0, "webin reads Sample KO G06", "0:150 1:150", "A:552572070;C:218631707;G:363017635;T:485997489;N:12099", 150, 150, null, null, 552572070, 218631707, 363017635, 485997489, 12099, "ERX14354925", "ERS24422933", "ERA33121297", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19307, "ERR14936889", "ERX14341131", "ERS24422756", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well B05", "Humanized Plate 2   Well B05", "SAMEA118242582", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample HUM2 B05|collection date:2022 12 21|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample HUM2 B05|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample HUM2 B05", "webin reads Sample HUM2 B05", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample HUM2 B05", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "B05.R1.fastq.gz B05.R2.fastq.gz", "fastq fastq", 9672318000.0, 32241060.0, "webin reads Sample HUM2 B05", "0:150 1:150", "A:3355070462;C:1344914308;G:2133672630;T:2838610016;N:50584", 150, 150, null, null, 3355070462, 1344914308, 2133672630, 2838610016, 50584, "ERX14341131", "ERS24422756", "ERA33115569", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "mate1-mate2 similar by mapping diff", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19308, "ERR14936826", "ERX14341068", "ERS24422749", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well A10", "Humanized Plate 2   Well A10", "SAMEA118242575", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample HUM2 A10|collection date:2022 12 21|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample HUM2 A10|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample HUM2 A10", "webin reads Sample HUM2 A10", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample HUM2 A10", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "A10.R1.fastq.gz A10.R2.fastq.gz", "fastq fastq", 3319486500.0, 11064955.0, "webin reads Sample HUM2 A10", "0:150 1:150", "A:667756780;C:505774653;G:1498623562;T:647316121;N:15384", 150, 150, null, null, 667756780, 505774653, 1498623562, 647316121, 15384, "ERX14341068", "ERS24422749", "ERA33115516", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "T", "T", "mates < 9% mapping rate", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19309, "ERR14950797", "ERX14354921", "ERS24422931", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well G04", "Knockout Plate   Well G04", "SAMEA118242757", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample KO G04|collection date:2022 05 13|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample KO G04|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample KO G04", "webin reads Sample KO G04", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample KO G04", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "G04.R1.fastq.gz G04.R2.fastq.gz", "fastq fastq", 1169241600.0, 3897472.0, "webin reads Sample KO G04", "0:150 1:150", "A:414405945;C:160380699;G:232288284;T:362157277;N:9395", 150, 150, null, null, 414405945, 160380699, 232288284, 362157277, 9395, "ERX14354921", "ERS24422931", "ERA33121293", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19310, "ERR14935076", "ERX14339318", "ERS24360081", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well F12", "Humanized Plate 1   Well F12", "SAMEA118225004", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample HUM1 F12|collection date:2022 11 14|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample HUM1 F12|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample HUM1 F12", "webin reads Sample HUM1 F12", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample HUM1 F12", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "F12.R1.fastq.gz F12.R2.fastq.gz", "fastq fastq", 460315800.0, 1534386.0, "webin reads Sample HUM1 F12", "0:150 1:150", "A:151663513;C:57564329;G:101614217;T:149469927;N:3814", 150, 150, null, null, 151663513, 57564329, 101614217, 149469927, 3814, "ERX14339318", "ERS24360081", "ERA33112847", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "mate1-mate2 similar by mapping diff", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19311, "ERR14950818", "ERX14354942", "ERS24422939", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well G12", "Knockout Plate   Well G12", "SAMEA118242765", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample KO G12|collection date:2022 05 13|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample KO G12|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample KO G12", "webin reads Sample KO G12", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample KO G12", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "G12.R1.fastq.gz G12.R2.fastq.gz", "fastq fastq", 413542200.0, 1378474.0, "webin reads Sample KO G12", "0:150 1:150", "A:126933895;C:59697863;G:113997315;T:112909944;N:3183", 150, 150, null, null, 126933895, 59697863, 113997315, 112909944, 3183, "ERX14354942", "ERS24422939", "ERA33121314", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19312, "ERR14950814", "ERX14354938", "ERS24422937", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well G10", "Knockout Plate   Well G10", "SAMEA118242763", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample KO G10|collection date:2022 05 13|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample KO G10|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample KO G10", "webin reads Sample KO G10", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample KO G10", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "G10.R1.fastq.gz G10.R2.fastq.gz", "fastq fastq", 4954517100.0, 16515057.0, "webin reads Sample KO G10", "0:150 1:150", "A:1782759867;C:666395120;G:897330151;T:1607991435;N:40527", 150, 150, null, null, 1782759867, 666395120, 897330151, 1607991435, 40527, "ERX14354938", "ERS24422937", "ERA33121310", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19313, "ERR14937147", "ERX14341391", "ERS24422817", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well G06", "Humanized Plate 2   Well G06", "SAMEA118242643", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample HUM2 G06|collection date:2022 12 21|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample HUM2 G06|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample HUM2 G06", "webin reads Sample HUM2 G06", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample HUM2 G06", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "G06.R1.fastq.gz G06.R2.fastq.gz", "fastq fastq", 2593562100.0, 8645207.0, "webin reads Sample HUM2 G06", "0:150 1:150", "A:506680806;C:379714116;G:1209306962;T:497848288;N:11928", 150, 150, null, null, 506680806, 379714116, 1209306962, 497848288, 11928, "ERX14341391", "ERS24422817", "ERA33115717", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "T", "T", "mates < 9% mapping rate", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19314, "ERR14934945", "ERX14339187", "ERS24360040", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well C07", "Humanized Plate 1   Well C07", "SAMEA118224963", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample HUM1 C07|collection date:2022 11 14|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample HUM1 C07|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample HUM1 C07", "webin reads Sample HUM1 C07", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample HUM1 C07", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "C07.R1.fastq.gz C07.R2.fastq.gz", "fastq fastq", 790847700.0, 2636159.0, "webin reads Sample HUM1 C07", "0:150 1:150", "A:263705480;C:97469638;G:157169342;T:272496337;N:6903", 150, 150, null, null, 263705480, 97469638, 157169342, 272496337, 6903, "ERX14339187", "ERS24360040", "ERA33112383", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19315, "ERR14937112", "ERX14341356", "ERS24422805", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well F06", "Humanized Plate 2   Well F06", "SAMEA118242631", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample HUM2 F06|collection date:2022 12 21|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample HUM2 F06|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample HUM2 F06", "webin reads Sample HUM2 F06", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample HUM2 F06", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "F06.R1.fastq.gz F06.R2.fastq.gz", "fastq fastq", 3966322200.0, 13221074.0, "webin reads Sample HUM2 F06", "0:150 1:150", "A:1063715250;C:596736050;G:1376244952;T:929607538;N:18410", 150, 150, null, null, 1063715250, 596736050, 1376244952, 929607538, 18410, "ERX14341356", "ERS24422805", "ERA33115694", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "T", "T", "mates < 9% mapping rate", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19316, "ERR14936848", "ERX14341090", "ERS24422752", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. We used a complete telomere to telomere genome sequence to identify 213 human specific gene families. From these  362 paralogs were found in all modern human genomes tested and brain transcriptomes  making them top candidates contributing to human universal brain features. Choosing a subset of paralogs  we used long read DNA sequencing of hundreds of modern humans to reveal previously hidden signatures of selection. To understand their roles in brain development  we generated zebrafish CRISPR \u201cknockout\u201d models of nine orthologs and introduced mRNA encoding paralogs  effectively \u201chumanizing\u201d larvae. Our findings implicate two new genes in possibly contributing to hallmark features of the human brain: GPR89B in dosage mediated brain expansion and FRMPD2B in altered synapse signaling. Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well B01", "Humanized Plate 2   Well B01", "SAMEA118242578", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample HUM2 B01|collection date:2022 12 21|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample HUM2 B01|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample HUM2 B01", "webin reads Sample HUM2 B01", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample HUM2 B01", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", "B01.R1.fastq.gz B01.R2.fastq.gz", "fastq fastq", 3646476600.0, 12154922.0, "webin reads Sample HUM2 B01", "0:150 1:150", "A:707531998;C:558638966;G:1658431639;T:721858281;N:15716", 150, 150, null, null, 707531998, 558638966, 1658431639, 721858281, 15716, "ERX14341090", "ERS24422752", "ERA33115539", "UNIVERSITY OF CALIFORNIA - DAVIS|European Nucleotide Archive", "UNIVERSITY OF CALIFORNIA - DAVIS", null, null, null, null, null, null, null, null, null, null, null, "T", "T", "mates < 9% mapping rate", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "sc", "single_cell_generic", "generic-scrnaseq-only", null, "United States", "2025-05-23", "Larval", "Larval", "Head", "Nervous System"], [19317, "ERR14937156", "ERX14341400", "ERS24422826", "ERP166068", "PRJEB82358", "Human gene duplications", "53f6d331-4985-4b69-8ac3-a57d010118dd", "Other", "Duplicated genes expanded in the human lineage likely contributed to brain evolution  yet challenges exist in their discovery due to sequence assembly errors. 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Our holistic approach provides new insights and a comprehensive resource for studying gene expansion drivers of human brain evolution.", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 05 23|ENA LAST UPDATE:2025 05 23", null, "Single cell RNA sequencing of zebrafish heads from well H03", "Humanized Plate 2   Well H03", "SAMEA118242652", "UNIVERSITY OF CALIFORNIA - DAVIS", "ENA first public:2025 05 23|INSDC center name:UNIVERSITY OF CALIFORNIA   DAVIS|INSDC status:public|Submitter Id:Sample HUM2 H03|collection date:2022 12 21|common name:zebrafish|dev stage:72 hpf|geographic location country and/or sea:USA|sample name:Sample HUM2 H03|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Raw reads: Sample HUM2 H03", "webin reads Sample HUM2 H03", null, "unspecified", null, "ENA STATUS ID:4", "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP166068", "Raw reads: Sample HUM2 H03", "ENA STATUS ID:4|ENA FIRST PUBLIC:2025 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