{"database": "metadata", "table": "run_metadata", "is_view": false, "human_description_en": "where experiment.library_selection = \"unspecified\", experiment.library_strategy = \"FL-cDNA\" and tissue_curation = \"Embryo Imprecise\"", "rows": [[8055, "ERR022484", "ERX008924", "ERS017427", "ERP000400", "PRJEB2333", "Sequencing the Zebrafish transcriptome form a range of tissues and developmental stages using the Illumina Genome Analyzer", "E-MTAB-434", "Other", null, null, null, null, "E MTAB 434:ZF 2cells", "SAMEA898400", "Wellcome Sanger Institute", "Alias:E MTAB 434:ZF 2cells|Broker name:ArrayExpress|Description:Protocols: Zebrafish embyos or tissues were collected from a Tuebingen strain incross and grown at 28 C. Collected samples were snap frozen on dry ice and stored at  70 C Total RNA was extracted using Trizol Reagent Invitrogen following the manufacturer's instructions. Pellets were resuspended RNase free 10 mM Tris pH 7.5 and the RNA was quantified using a NanoDrop ND 1000 Spectrophotometer Axon Instruments. Total RNA was made into an RNAseq Illumina library following the manufacturer's protocol including a DNase treatment between to 2 rounds of polyA pull down. 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