{"database": "metadata", "table": "run_metadata", "is_view": false, "human_description_en": "where experiment.library_selection = \"other\" and tissue_curation_coarse = \"Renal System\"", "rows": [[53291, "SRR9849281", "SRX6603894", "SRS5168943", "SRP216556", "PRJNA556842", "aKlotho regulates age associated vascular calcification and lifespan in zebrafish", "PRJNA556842", "Transcriptome Analysis", "The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast  its overexpression increases lifespan. Given the severe mouse phenotype  we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish  with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body  most dramatically in the outflow tract of the heart  the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.", null, null, null, "wildtype  kidney  5 mpf  replicate 5", "wt 14560 b kidney", null, "strain:AB|age:5months|sex:female|tissue:kidney|genotype:wildtype|Replicate:Bilogical replicate 5|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "wildtype  kidney  5 mpf  replicate 5", "wt 14560 b kidney", "wt 14560 b kidney", "Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. 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RNALater was removed post an overnight incubation at 4C  and samples were stored at  80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing  mercaptoethanol and stored at  80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. 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RNALater was removed post an overnight incubation at 4C  and samples were stored at  80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing  mercaptoethanol and stored at  80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. 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RNALater was removed post an overnight incubation at 4C  and samples were stored at  80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing  mercaptoethanol and stored at  80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP216556", null, null, "kl-14744-d-kidney_merged_S94-1.fastq.gz kl-14744-d-kidney_merged_S94-2.fastq.gz", "fastq fastq", 3235564566.0, 21704627.0, "kl 14744 d kidney merged S94 1.fastq.gz", "0:74.55 1:74.53", "A:808568448;C:783575935;G:776803253;T:830140556;N:36476374", 74, 74, null, null, 808568448, 783575935, 776803253, 830140556, 36476374, "SRX6603887", "SRS5168935", "SRA927891", "Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics", "Novartis Institutes for Biomedical Research", 2, 0.95065, 0.95457, 0.07932, 0.0782, 0.69844, 0.69852, 0.52161, 0.52384, 75, 75, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "Germany", "2019-07-27", "Adult", "Adult", "Kidney", "Renal System"], [53299, "SRR9849200", "SRX6603886", "SRS5168936", "SRP216556", "PRJNA556842", "aKlotho regulates age associated vascular calcification and lifespan in zebrafish", "PRJNA556842", "Transcriptome Analysis", "The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast  its overexpression increases lifespan. Given the severe mouse phenotype  we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish  with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body  most dramatically in the outflow tract of the heart  the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.", null, null, null, "\u03b1Klotho  kidney  5 mpf  replicate 7", "kl 14563 b kidney", null, "strain:AB|age:5months|sex:female|tissue:kidney|genotype:aKlotho|Replicate:Bilogical replicate 7|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Klotho  kidney  5 mpf  replicate 7", "kl 14563 b kidney", "kl 14563 b kidney", "Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills  heart and kidney were collected from 32 individual fish at two time points  namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point   4 males  4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C  and samples were stored at  80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing  mercaptoethanol and stored at  80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP216556", null, null, "kl-14563-b-kidney_merged_S23-1.fastq.gz kl-14563-b-kidney_merged_S23-2.fastq.gz", "fastq fastq", 3841497499.0, 25646923.0, "kl 14563 b kidney merged S23 1.fastq.gz", "0:74.90 1:74.89", "A:999048603;C:899322616;G:891418096;T:1023530696;N:28177488", 74, 74, null, null, 999048603, 899322616, 891418096, 1023530696, 28177488, "SRX6603886", "SRS5168936", "SRA927891", "Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics", "Novartis Institutes for Biomedical Research", 2, 0.94562, 0.94897, 0.098, 0.09621, 0.71977, 0.7209, 0.54321, 0.53814, 76, 75, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "Germany", "2019-07-27", "Adult", "Adult", "Kidney", "Renal System"], [53300, "SRR9849206", "SRX6603885", "SRS5168934", "SRP216556", "PRJNA556842", "aKlotho regulates age associated vascular calcification and lifespan in zebrafish", "PRJNA556842", "Transcriptome Analysis", "The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast  its overexpression increases lifespan. Given the severe mouse phenotype  we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish  with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body  most dramatically in the outflow tract of the heart  the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.", null, null, null, "\u03b1Klotho  kidney  5 mpf  replicate 8", "kl 14563 c kidney", null, "strain:AB|age:5months|sex:female|tissue:kidney|genotype:aKlotho|Replicate:Bilogical replicate 8|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Klotho  kidney  5 mpf  replicate 8", "kl 14563 c kidney", "kl 14563 c kidney", "Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills  heart and kidney were collected from 32 individual fish at two time points  namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point   4 males  4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C  and samples were stored at  80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing  mercaptoethanol and stored at  80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP216556", null, null, "kl-14563-c-kidney_merged_S35-1.fastq.gz kl-14563-c-kidney_merged_S35-2.fastq.gz", "fastq fastq", 4215569581.0, 28089663.0, "kl 14563 c kidney merged S35 1.fastq.gz", "0:75.05 1:75.03", "A:1122288966;C:970063144;G:955510223;T:1146500593;N:21206655", 75, 75, null, null, 1122288966, 970063144, 955510223, 1146500593, 21206655, "SRX6603885", "SRS5168934", "SRA927891", "Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics", "Novartis Institutes for Biomedical Research", 2, 0.94171, 0.94373, 0.11361, 0.11133, 0.70136, 0.7025, 0.49345, 0.51472, 76, 76, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "Germany", "2019-07-27", "Adult", "Adult", "Kidney", "Renal System"], [53301, "SRR9849201", "SRX6603884", "SRS5168933", "SRP216556", "PRJNA556842", "aKlotho regulates age associated vascular calcification and lifespan in zebrafish", "PRJNA556842", "Transcriptome Analysis", "The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast  its overexpression increases lifespan. Given the severe mouse phenotype  we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish  with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body  most dramatically in the outflow tract of the heart  the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.", null, null, null, "\u03b1Klotho  kidney  5 mpf  replicate 5", "kl 14562 b kidney", null, "strain:AB|age:5months|sex:female|tissue:kidney|genotype:aKlotho|Replicate:Bilogical replicate 5|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Klotho  kidney  5 mpf  replicate 5", "kl 14562 b kidney", "kl 14562 b kidney", "Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills  heart and kidney were collected from 32 individual fish at two time points  namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point   4 males  4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C  and samples were stored at  80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing  mercaptoethanol and stored at  80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP216556", null, null, "kl-14562-b-kidney_merged_S18-1.fastq.gz kl-14562-b-kidney_merged_S18-2.fastq.gz", "fastq fastq", 3972193490.0, 27155081.0, "kl 14562 b kidney merged S18 1.fastq.gz", "0:73.14 1:73.14", "A:987358107;C:933387787;G:924448548;T:1015576411;N:111422637", 73, 73, null, null, 987358107, 933387787, 924448548, 1015576411, 111422637, "SRX6603884", "SRS5168933", "SRA927891", "Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics", "Novartis Institutes for Biomedical Research", 2, 0.94949, 0.95176, 0.08272, 0.08121, 0.7166, 0.71705, 0.53783, 0.53989, 76, 76, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "Germany", "2019-07-27", "Adult", "Adult", "Kidney", "Renal System"], [53302, "SRR9849205", "SRX6603883", "SRS5168932", "SRP216556", "PRJNA556842", "aKlotho regulates age associated vascular calcification and lifespan in zebrafish", "PRJNA556842", "Transcriptome Analysis", "The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast  its overexpression increases lifespan. Given the severe mouse phenotype  we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish  with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body  most dramatically in the outflow tract of the heart  the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.", null, null, null, "\u03b1Klotho  kidney  5 mpf  replicate 6", "kl 14562 d kidney", null, "strain:AB|age:5months|sex:female|tissue:kidney|genotype:aKlotho|Replicate:Bilogical replicate 6|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Klotho  kidney  5 mpf  replicate 6", "kl 14562 d kidney", "kl 14562 d kidney", "Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills  heart and kidney were collected from 32 individual fish at two time points  namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point   4 males  4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C  and samples were stored at  80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing  mercaptoethanol and stored at  80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP216556", null, null, "kl-14562-d-kidney_merged_S42-1.fastq.gz kl-14562-d-kidney_merged_S42-2.fastq.gz", "fastq fastq", 3526368194.0, 23516128.0, "kl 14562 d kidney merged S42 1.fastq.gz", "0:75.00 1:74.95", "A:869319867;C:871562247;G:869066601;T:893717214;N:22702265", 75, 74, null, null, 869319867, 871562247, 869066601, 893717214, 22702265, "SRX6603883", "SRS5168932", "SRA927891", "Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics", "Novartis Institutes for Biomedical Research", 2, 0.96175, 0.96369, 0.06361, 0.06218, 0.70905, 0.71157, 0.51899, 0.52384, 76, 76, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "Germany", "2019-07-27", "Adult", "Adult", "Kidney", "Renal System"], [53303, "SRR9849202", "SRX6603882", "SRS5168931", "SRP216556", "PRJNA556842", "aKlotho regulates age associated vascular calcification and lifespan in zebrafish", "PRJNA556842", "Transcriptome Analysis", "The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast  its overexpression increases lifespan. Given the severe mouse phenotype  we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish  with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body  most dramatically in the outflow tract of the heart  the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.", null, null, null, "\u03b1Klotho  kidney  5 mpf  replicate 3", "kl 14563 a kidney", null, "strain:AB|age:5months|sex:male|tissue:kidney|genotype:aKlotho|Replicate:Bilogical replicate 3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Klotho  kidney  5 mpf  replicate 3", "kl 14563 a kidney", "kl 14563 a kidney", "Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills  heart and kidney were collected from 32 individual fish at two time points  namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point   4 males  4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C  and samples were stored at  80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing  mercaptoethanol and stored at  80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP216556", null, null, "kl-14563-a-kidney_merged_S11-1.fastq.gz kl-14563-a-kidney_merged_S11-2.fastq.gz", "fastq fastq", 4326402378.0, 29503938.0, "kl 14563 a kidney merged S11 1.fastq.gz", "0:73.31 1:73.33", "A:1085969692;C:1017480039;G:1010619557;T:1105911425;N:106421665", 73, 73, null, null, 1085969692, 1017480039, 1010619557, 1105911425, 106421665, "SRX6603882", "SRS5168931", "SRA927891", "Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics", "Novartis Institutes for Biomedical Research", 2, 0.94169, 0.94864, 0.08735, 0.0856, 0.74018, 0.74063, 0.532, 0.54233, 35, 35, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "Germany", "2019-07-27", "Adult", "Adult", "Kidney", "Renal System"], [53304, "SRR9849203", "SRX6603881", "SRS5168930", "SRP216556", "PRJNA556842", "aKlotho regulates age associated vascular calcification and lifespan in zebrafish", "PRJNA556842", "Transcriptome Analysis", "The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast  its overexpression increases lifespan. Given the severe mouse phenotype  we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish  with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body  most dramatically in the outflow tract of the heart  the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.", null, null, null, "\u03b1Klotho  kidney  5 mpf  replicate 1", "kl 14562 a kidney", null, "strain:AB|age:5months|sex:male|tissue:kidney|genotype:aKlotho|Replicate:Bilogical replicate 1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Klotho  kidney  5 mpf  replicate 1", "kl 14562 a kidney", "kl 14562 a kidney", "Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills  heart and kidney were collected from 32 individual fish at two time points  namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point   4 males  4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C  and samples were stored at  80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing  mercaptoethanol and stored at  80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP216556", null, null, "kl-14562-a-kidney_merged_S6-1.fastq.gz kl-14562-a-kidney_merged_S6-2.fastq.gz", "fastq fastq", 4980501349.0, 34004455.0, "kl 14562 a kidney merged S6 1.fastq.gz", "0:73.23 1:73.24", "A:1251556852;C:1167350759;G:1159943717;T:1277350171;N:124299850", 73, 73, null, null, 1251556852, 1167350759, 1159943717, 1277350171, 124299850, "SRX6603881", "SRS5168930", "SRA927891", "Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics", "Novartis Institutes for Biomedical Research", 2, 0.94619, 0.94971, 0.08823, 0.08683, 0.72807, 0.72796, 0.51972, 0.52145, 35, 35, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "Germany", "2019-07-27", "Adult", "Adult", "Kidney", "Renal System"], [53305, "SRR9849204", "SRX6603880", "SRS5168929", "SRP216556", "PRJNA556842", "aKlotho regulates age associated vascular calcification and lifespan in zebrafish", "PRJNA556842", "Transcriptome Analysis", "The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast  its overexpression increases lifespan. Given the severe mouse phenotype  we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish  with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body  most dramatically in the outflow tract of the heart  the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.", null, null, null, "\u03b1Klotho  kidney  5 mpf  replicate 4", "kl 14563 d kidney", null, "strain:AB|age:5months|sex:male|tissue:kidney|genotype:aKlotho|Replicate:Bilogical replicate 4|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Klotho  kidney  5 mpf  replicate 4", "kl 14563 d kidney", "kl 14563 d kidney", "Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills  heart and kidney were collected from 32 individual fish at two time points  namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point   4 males  4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C  and samples were stored at  80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing  mercaptoethanol and stored at  80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP216556", null, null, "kl-14563-d-kidney_merged_S47-1.fastq.gz kl-14563-d-kidney_merged_S47-2.fastq.gz", "fastq fastq", 6869441307.0, 56338972.0, "kl 14563 d kidney merged S47 1.fastq.gz", "0:60.76 1:61.17", "A:1348721440;C:1368998259;G:1366726420;T:1380954564;N:1404040624", 60, 61, null, null, 1348721440, 1368998259, 1366726420, 1380954564, 1404040624, "SRX6603880", "SRS5168929", "SRA927891", "Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics", "Novartis Institutes for Biomedical Research", 2, 0.96257, 0.96453, 0.06751, 0.06671, 0.75548, 0.75607, 0.51978, 0.52832, 74, 76, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "Germany", "2019-07-27", "Adult", "Adult", "Kidney", "Renal System"], [53306, "SRR9849207", "SRX6603879", "SRS5168928", "SRP216556", "PRJNA556842", "aKlotho regulates age associated vascular calcification and lifespan in zebrafish", "PRJNA556842", "Transcriptome Analysis", "The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast  its overexpression increases lifespan. Given the severe mouse phenotype  we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish  with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body  most dramatically in the outflow tract of the heart  the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.", null, null, null, "\u03b1Klotho  kidney  5 mpf  replicate 2", "kl 14562 c kidney", null, "strain:AB|age:5months|sex:male|tissue:kidney|genotype:aKlotho|Replicate:Bilogical replicate 2|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Klotho  kidney  5 mpf  replicate 2", "kl 14562 c kidney", "kl 14562 c kidney", "Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills  heart and kidney were collected from 32 individual fish at two time points  namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point   4 males  4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C  and samples were stored at  80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing  mercaptoethanol and stored at  80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP216556", null, null, "kl-14562-c-kidney_merged_S30-1.fastq.gz kl-14562-c-kidney_merged_S30-2.fastq.gz", "fastq fastq", 3320801196.0, 22339756.0, "kl 14562 c kidney merged S30 1.fastq.gz", "0:74.33 1:74.32", "A:856878404;C:774178244;G:769653230;T:875894981;N:44196337", 74, 74, null, null, 856878404, 774178244, 769653230, 875894981, 44196337, "SRX6603879", "SRS5168928", "SRA927891", "Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics", "Novartis Institutes for Biomedical Research", 2, 0.95411, 0.95756, 0.08333, 0.08245, 0.7162, 0.71638, 0.56576, 0.55653, 74, 76, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "Germany", "2019-07-27", "Adult", "Adult", "Kidney", "Renal System"], [53307, "SRR9849213", "SRX6603878", "SRS5168927", "SRP216556", "PRJNA556842", "aKlotho regulates age associated vascular calcification and lifespan in zebrafish", "PRJNA556842", "Transcriptome Analysis", "The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast  its overexpression increases lifespan. Given the severe mouse phenotype  we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish  with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body  most dramatically in the outflow tract of the heart  the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.", null, null, null, "\u03b1Klotho  kidney  3 mpf  replicate 6", "kl 14744 f kidney", null, "strain:AB|age:3months|sex:female|tissue:kidney|genotype:aKlotho|Replicate:Bilogical replicate 6|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Klotho  kidney  3 mpf  replicate 6", "kl 14744 f kidney", "kl 14744 f kidney", "Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills  heart and kidney were collected from 32 individual fish at two time points  namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point   4 males  4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C  and samples were stored at  80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing  mercaptoethanol and stored at  80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP216556", null, null, "kl-14744-f-kidney_merged_S71-1.fastq.gz kl-14744-f-kidney_merged_S71-2.fastq.gz", "fastq fastq", 2801226765.0, 18731091.0, "kl 14744 f kidney merged S71 1.fastq.gz", "0:74.79 1:74.76", "A:720950274;C:664580809;G:653567624;T:738645101;N:23482957", 74, 74, null, null, 720950274, 664580809, 653567624, 738645101, 23482957, "SRX6603878", "SRS5168927", "SRA927891", "Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics", "Novartis Institutes for Biomedical Research", 2, 0.94692, 0.95016, 0.10024, 0.09917, 0.69781, 0.69745, 0.53063, 0.53615, 76, 76, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "Germany", "2019-07-27", "Adult", "Adult", "Kidney", "Renal System"], [53308, "SRR9849208", "SRX6603877", "SRS5168926", "SRP216556", "PRJNA556842", "aKlotho regulates age associated vascular calcification and lifespan in zebrafish", "PRJNA556842", "Transcriptome Analysis", "The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast  its overexpression increases lifespan. Given the severe mouse phenotype  we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish  with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body  most dramatically in the outflow tract of the heart  the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.", null, null, null, "\u03b1Klotho  kidney  3 mpf  replicate 7", "kl 14744 h kidney", null, "strain:AB|age:3months|sex:female|tissue:kidney|genotype:aKlotho|Replicate:Bilogical replicate 7|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Klotho  kidney  3 mpf  replicate 7", "kl 14744 h kidney", "kl 14744 h kidney", "Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills  heart and kidney were collected from 32 individual fish at two time points  namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point   4 males  4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C  and samples were stored at  80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing  mercaptoethanol and stored at  80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP216556", null, null, "kl-14744-h-kidney_merged_S95-1.fastq.gz kl-14744-h-kidney_merged_S95-2.fastq.gz", "fastq fastq", 3606112938.0, 24298531.0, "kl 14744 h kidney merged S95 1.fastq.gz", "0:74.21 1:74.20", "A:907594636;C:863534560;G:852256795;T:929423242;N:53303705", 74, 74, null, null, 907594636, 863534560, 852256795, 929423242, 53303705, "SRX6603877", "SRS5168926", "SRA927891", "Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics", "Novartis Institutes for Biomedical Research", 2, 0.95343, 0.95612, 0.07895, 0.07817, 0.70944, 0.71023, 0.54468, 0.54382, 76, 76, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "Germany", "2019-07-27", "Adult", "Adult", "Kidney", "Renal System"], [53309, "SRR9849212", "SRX6603876", "SRS5168925", "SRP216556", "PRJNA556842", "aKlotho regulates age associated vascular calcification and lifespan in zebrafish", "PRJNA556842", "Transcriptome Analysis", "The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast  its overexpression increases lifespan. Given the severe mouse phenotype  we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish  with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body  most dramatically in the outflow tract of the heart  the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.", null, null, null, "wildtype  kidney  5 mpf  replicate 2", "wt 14560 c kidney", null, "strain:AB|age:5months|sex:male|tissue:kidney|genotype:wildtype|Replicate:Bilogical replicate 2|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "wildtype  kidney  5 mpf  replicate 2", "wt 14560 c kidney", "wt 14560 c kidney", "Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills  heart and kidney were collected from 32 individual fish at two time points  namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point   4 males  4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C  and samples were stored at  80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing  mercaptoethanol and stored at  80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP216556", null, null, "wt-14560-c-kidney_merged_S29-1.fastq.gz wt-14560-c-kidney_merged_S29-2.fastq.gz", "fastq fastq", 3091657773.0, 21774524.0, "wt 14560 c kidney merged S29 1.fastq.gz", "0:70.96 1:71.03", "A:763113194;C:693515052;G:687380502;T:778507279;N:169141746", 70, 71, null, null, 763113194, 693515052, 687380502, 778507279, 169141746, "SRX6603876", "SRS5168925", "SRA927891", "Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics", "Novartis Institutes for Biomedical Research", 2, 0.95062, 0.95349, 0.09247, 0.09119, 0.72754, 0.72863, 0.53643, 0.54181, 76, 76, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "Germany", "2019-07-27", "Adult", "Adult", "Kidney", "Renal System"], [53310, "SRR9849209", "SRX6603875", "SRS5168924", "SRP216556", "PRJNA556842", "aKlotho regulates age associated vascular calcification and lifespan in zebrafish", "PRJNA556842", "Transcriptome Analysis", "The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast  its overexpression increases lifespan. Given the severe mouse phenotype  we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish  with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body  most dramatically in the outflow tract of the heart  the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.", null, null, null, "wildtype  kidney  5 mpf  replicate 3", "wt 14560 e kidney", null, "strain:AB|age:5months|sex:male|tissue:kidney|genotype:wildtype|Replicate:Bilogical replicate 3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "wildtype  kidney  5 mpf  replicate 3", "wt 14560 e kidney", "wt 14560 e kidney", "Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills  heart and kidney were collected from 32 individual fish at two time points  namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point   4 males  4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C  and samples were stored at  80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing  mercaptoethanol and stored at  80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP216556", null, null, "wt-14560-e-kidney_merged_S53-1.fastq.gz wt-14560-e-kidney_merged_S53-2.fastq.gz", "fastq fastq", 3242753931.0, 21639832.0, "wt 14560 e kidney merged S53 1.fastq.gz", "0:74.93 1:74.92", "A:843085674;C:760074682;G:756945733;T:860752379;N:21895463", 74, 74, null, null, 843085674, 760074682, 756945733, 860752379, 21895463, "SRX6603875", "SRS5168924", "SRA927891", "Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics", "Novartis Institutes for Biomedical Research", 2, 0.95058, 0.95272, 0.0982, 0.09719, 0.71707, 0.71825, 0.5497, 0.53642, 75, 76, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "Germany", "2019-07-27", "Adult", "Adult", "Kidney", "Renal System"], [53311, "SRR9849210", "SRX6603874", "SRS5168923", "SRP216556", "PRJNA556842", "aKlotho regulates age associated vascular calcification and lifespan in zebrafish", "PRJNA556842", "Transcriptome Analysis", "The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast  its overexpression increases lifespan. Given the severe mouse phenotype  we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish  with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body  most dramatically in the outflow tract of the heart  the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.", null, null, null, "wildtype  kidney  3 mpf  replicate 7", "wt 14746 g kidney", null, "strain:AB|age:3months|sex:female|tissue:kidney|genotype:wildtype|Replicate:Bilogical replicate 7|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "wildtype  kidney  3 mpf  replicate 7", "wt 14746 g kidney", "wt 14746 g kidney", "Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills  heart and kidney were collected from 32 individual fish at two time points  namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point   4 males  4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C  and samples were stored at  80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing  mercaptoethanol and stored at  80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP216556", null, null, "wt-14746-g-kidney_merged_S22-1.fastq.gz wt-14746-g-kidney_merged_S22-2.fastq.gz", "fastq fastq", 3928427553.0, 26318173.0, "wt 14746 g kidney merged S22 1.fastq.gz", "0:74.63 1:74.64", "A:1019611627;C:916883865;G:906743219;T:1044351913;N:40836929", 74, 74, null, null, 1019611627, 916883865, 906743219, 1044351913, 40836929, "SRX6603874", "SRS5168923", "SRA927891", "Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics", "Novartis Institutes for Biomedical Research", 2, 0.94281, 0.94563, 0.09937, 0.0975, 0.71478, 0.71585, 0.54524, 0.5444, 76, 74, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "Germany", "2019-07-27", "Adult", "Adult", "Kidney", "Renal System"], [53312, "SRR9849211", "SRX6603873", "SRS5168922", "SRP216556", "PRJNA556842", "aKlotho regulates age associated vascular calcification and lifespan in zebrafish", "PRJNA556842", "Transcriptome Analysis", "The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast  its overexpression increases lifespan. Given the severe mouse phenotype  we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish  with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body  most dramatically in the outflow tract of the heart  the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.", null, null, null, "wildtype  kidney  3 mpf  replicate 6", "wt 14746 d kidney", null, "strain:AB|age:3months|sex:female|tissue:kidney|genotype:wildtype|Replicate:Bilogical replicate 6|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "wildtype  kidney  3 mpf  replicate 6", "wt 14746 d kidney", "wt 14746 d kidney", "Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills  heart and kidney were collected from 32 individual fish at two time points  namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point   4 males  4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C  and samples were stored at  80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing  mercaptoethanol and stored at  80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP216556", null, null, "wt-14746-d-kidney_merged_S81-2.fastq.gz wt-14746-d-kidney_merged_S81-1.fastq.gz", "fastq fastq", 3235839633.0, 21863802.0, "wt 14746 d kidney merged S81 1.fastq.gz", "0:73.99 1:74.01", "A:819433052;C:762877585;G:759574301;T:837534821;N:56419874", 73, 74, null, null, 819433052, 762877585, 759574301, 837534821, 56419874, "SRX6603873", "SRS5168922", "SRA927891", "Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics", "Novartis Institutes for Biomedical Research", 2, 0.94986, 0.95295, 0.09789, 0.09661, 0.72109, 0.72344, 0.5002, 0.53439, 75, 75, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "Germany", "2019-07-27", "Adult", "Adult", "Kidney", "Renal System"], [53313, "SRR9849214", "SRX6603872", "SRS5168921", "SRP216556", "PRJNA556842", "aKlotho regulates age associated vascular calcification and lifespan in zebrafish", "PRJNA556842", "Transcriptome Analysis", "The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast  its overexpression increases lifespan. Given the severe mouse phenotype  we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish  with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body  most dramatically in the outflow tract of the heart  the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.", null, null, null, "wildtype  kidney  3 mpf  replicate 8", "wt 14746 i kidney", null, "strain:AB|age:3months|sex:female|tissue:kidney|genotype:wildtype|Replicate:Bilogical replicate 8|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "wildtype  kidney  3 mpf  replicate 8", "wt 14746 i kidney", "wt 14746 i kidney", "Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills  heart and kidney were collected from 32 individual fish at two time points  namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point   4 males  4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C  and samples were stored at  80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing  mercaptoethanol and stored at  80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP216556", null, null, "wt-14746-i-kidney_merged_S46-1.fastq.gz wt-14746-i-kidney_merged_S46-2.fastq.gz", "fastq fastq", 3377453037.0, 23023990.0, "wt 14746 i kidney merged S46 1.fastq.gz", "0:73.35 1:73.34", "A:836450075;C:803489724;G:795497926;T:858148995;N:83866317", 73, 73, null, null, 836450075, 803489724, 795497926, 858148995, 83866317, "SRX6603872", "SRS5168921", "SRA927891", "Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics", "Novartis Institutes for Biomedical Research", 2, 0.95192, 0.95558, 0.0795, 0.07926, 0.70589, 0.70619, 0.52743, 0.52432, 76, 76, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "Germany", "2019-07-27", "Adult", "Adult", "Kidney", "Renal System"], [53314, "SRR9849220", "SRX6603871", "SRS5168920", "SRP216556", "PRJNA556842", "aKlotho regulates age associated vascular calcification and lifespan in zebrafish", "PRJNA556842", "Transcriptome Analysis", "The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast  its overexpression increases lifespan. Given the severe mouse phenotype  we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish  with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body  most dramatically in the outflow tract of the heart  the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.", null, null, null, "wildtype  kidney  5 mpf  replicate 1", "wt 14560 a kidney", null, "strain:AB|age:5months|sex:male|tissue:kidney|genotype:wildtype|Replicate:Bilogical replicate 1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "wildtype  kidney  5 mpf  replicate 1", "wt 14560 a kidney", "wt 14560 a kidney", "Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills  heart and kidney were collected from 32 individual fish at two time points  namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point   4 males  4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C  and samples were stored at  80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing  mercaptoethanol and stored at  80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP216556", null, null, "wt-14560-a-kidney_merged_S5-1.fastq.gz wt-14560-a-kidney_merged_S5-2.fastq.gz", "fastq fastq", 3803097028.0, 29032173.0, "wt 14560 a kidney merged S5 1.fastq.gz", "0:65.34 1:65.66", "A:880043540;C:764446357;G:759630921;T:898342437;N:500633773", 65, 65, null, null, 880043540, 764446357, 759630921, 898342437, 500633773, "SRX6603871", "SRS5168920", "SRA927891", "Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics", "Novartis Institutes for Biomedical Research", 2, 0.94594, 0.94896, 0.10801, 0.10661, 0.74292, 0.74379, 0.56139, 0.56212, 76, 75, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "Germany", "2019-07-27", "Adult", "Adult", "Kidney", "Renal System"], [53315, "SRR9849215", "SRX6603870", "SRS5168919", "SRP216556", "PRJNA556842", "aKlotho regulates age associated vascular calcification and lifespan in zebrafish", "PRJNA556842", "Transcriptome Analysis", "The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast  its overexpression increases lifespan. Given the severe mouse phenotype  we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish  with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body  most dramatically in the outflow tract of the heart  the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.", null, null, null, "wildtype  kidney  3 mpf  replicate 2", "wt 14746 e kidney", null, "strain:AB|age:3months|sex:male|tissue:kidney|genotype:wildtype|Replicate:Bilogical replicate 2|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "wildtype  kidney  3 mpf  replicate 2", "wt 14746 e kidney", "wt 14746 e kidney", "Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills  heart and kidney were collected from 32 individual fish at two time points  namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point   4 males  4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C  and samples were stored at  80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing  mercaptoethanol and stored at  80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP216556", null, null, "wt-14746-e-kidney_merged_S93-1.fastq.gz wt-14746-e-kidney_merged_S93-2.fastq.gz", "fastq fastq", 3389715259.0, 23030176.0, "wt 14746 e kidney merged S93 1.fastq.gz", "0:73.58 1:73.60", "A:845566798;C:806510240;G:801049741;T:861758601;N:74829879", 73, 73, null, null, 845566798, 806510240, 801049741, 861758601, 74829879, "SRX6603870", "SRS5168919", "SRA927891", "Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics", "Novartis Institutes for Biomedical Research", 2, 0.96201, 0.96536, 0.07181, 0.0708, 0.73594, 0.73628, 0.5625, 0.57023, 76, 76, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "Germany", "2019-07-27", "Adult", "Adult", "Kidney", "Renal System"], [53316, "SRR9849219", "SRX6603869", "SRS5168918", "SRP216556", "PRJNA556842", "aKlotho regulates age associated vascular calcification and lifespan in zebrafish", "PRJNA556842", "Transcriptome Analysis", "The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast  its overexpression increases lifespan. Given the severe mouse phenotype  we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish  with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body  most dramatically in the outflow tract of the heart  the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.", null, null, null, "wildtype  kidney  3 mpf  replicate 3", "wt 14746 f kidney", null, "strain:AB|age:3months|sex:male|tissue:kidney|genotype:wildtype|Replicate:Bilogical replicate 3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "wildtype  kidney  3 mpf  replicate 3", "wt 14746 f kidney", "wt 14746 f kidney", "Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills  heart and kidney were collected from 32 individual fish at two time points  namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point   4 males  4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C  and samples were stored at  80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing  mercaptoethanol and stored at  80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP216556", null, null, "wt-14746-f-kidney_merged_S10-1.fastq.gz wt-14746-f-kidney_merged_S10-2.fastq.gz", "fastq fastq", 5697834151.0, 38061527.0, "wt 14746 f kidney merged S10 1.fastq.gz", "0:74.86 1:74.84", "A:1488491305;C:1328009157;G:1311260167;T:1524663985;N:45409537", 74, 74, null, null, 1488491305, 1328009157, 1311260167, 1524663985, 45409537, "SRX6603869", "SRS5168918", "SRA927891", "Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics", "Novartis Institutes for Biomedical Research", 2, 0.94873, 0.9525, 0.0943, 0.09336, 0.71709, 0.71741, 0.53907, 0.55371, 76, 75, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "Germany", "2019-07-27", "Adult", "Adult", "Kidney", "Renal System"], [53317, "SRR9849216", "SRX6603868", "SRS5168917", "SRP216556", "PRJNA556842", "aKlotho regulates age associated vascular calcification and lifespan in zebrafish", "PRJNA556842", "Transcriptome Analysis", "The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast  its overexpression increases lifespan. Given the severe mouse phenotype  we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish  with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body  most dramatically in the outflow tract of the heart  the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.", null, null, null, "wildtype  kidney  3 mpf  replicate 4", "wt 14746 h kidney", null, "strain:AB|age:3months|sex:male|tissue:kidney|genotype:wildtype|Replicate:Bilogical replicate 4|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "wildtype  kidney  3 mpf  replicate 4", "wt 14746 h kidney", "wt 14746 h kidney", "Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills  heart and kidney were collected from 32 individual fish at two time points  namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point   4 males  4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C  and samples were stored at  80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing  mercaptoethanol and stored at  80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP216556", null, null, "wt-14746-h-kidney_merged_S34-1.fastq.gz wt-14746-h-kidney_merged_S34-2.fastq.gz", "fastq fastq", 4802949250.0, 32003702.0, "wt 14746 h kidney merged S34 1.fastq.gz", "0:75.05 1:75.02", "A:1247408978;C:1132423247;G:1119030906;T:1277389500;N:26696619", 75, 75, null, null, 1247408978, 1132423247, 1119030906, 1277389500, 26696619, "SRX6603868", "SRS5168917", "SRA927891", "Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics", "Novartis Institutes for Biomedical Research", 2, 0.94868, 0.95207, 0.1008, 0.1002, 0.70654, 0.70583, 0.543, 0.53646, 76, 76, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "Germany", "2019-07-27", "Adult", "Adult", "Kidney", "Renal System"], [53318, "SRR9849217", "SRX6603867", "SRS5168916", "SRP216556", "PRJNA556842", "aKlotho regulates age associated vascular calcification and lifespan in zebrafish", "PRJNA556842", "Transcriptome Analysis", "The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast  its overexpression increases lifespan. Given the severe mouse phenotype  we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish  with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body  most dramatically in the outflow tract of the heart  the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.", null, null, null, "wildtype  kidney  3 mpf  replicate 1", "wt 14746 c kidney", null, "strain:AB|age:3months|sex:male|tissue:kidney|genotype:wildtype|Replicate:Bilogical replicate 1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "wildtype  kidney  3 mpf  replicate 1", "wt 14746 c kidney", "wt 14746 c kidney", "Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills  heart and kidney were collected from 32 individual fish at two time points  namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point   4 males  4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C  and samples were stored at  80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing  mercaptoethanol and stored at  80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP216556", null, null, "wt-14746-c-kidney_merged_S69-1.fastq.gz wt-14746-c-kidney_merged_S69-2.fastq.gz", "fastq fastq", 2926155789.0, 19616550.0, "wt 14746 c kidney merged S69 1.fastq.gz", "0:74.59 1:74.58", "A:738508985;C:703442172;G:697795154;T:755346949;N:31062529", 74, 74, null, null, 738508985, 703442172, 697795154, 755346949, 31062529, "SRX6603867", "SRS5168916", "SRA927891", "Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics", "Novartis Institutes for Biomedical Research", 2, 0.95391, 0.95737, 0.08073, 0.08025, 0.72273, 0.72265, 0.50256, 0.53781, 76, 75, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "Germany", "2019-07-27", "Adult", "Adult", "Kidney", "Renal System"], [53319, "SRR9849218", "SRX6603866", "SRS5168915", "SRP216556", "PRJNA556842", "aKlotho regulates age associated vascular calcification and lifespan in zebrafish", "PRJNA556842", "Transcriptome Analysis", "The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast  its overexpression increases lifespan. Given the severe mouse phenotype  we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish  with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body  most dramatically in the outflow tract of the heart  the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.", null, null, null, "wildtype  kidney  3 mpf  replicate 5", "wt 14746 b kidney", null, "strain:AB|age:3months|sex:female|tissue:kidney|genotype:wildtype|Replicate:Bilogical replicate 5|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "wildtype  kidney  3 mpf  replicate 5", "wt 14746 b kidney", "wt 14746 b kidney", "Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills  heart and kidney were collected from 32 individual fish at two time points  namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point   4 males  4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C  and samples were stored at  80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing  mercaptoethanol and stored at  80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP216556", null, null, "wt-14746-b-kidney_merged_S57-1.fastq.gz wt-14746-b-kidney_merged_S57-2.fastq.gz", "fastq fastq", 3580693879.0, 24148496.0, "wt 14746 b kidney merged S57 1.fastq.gz", "0:74.13 1:74.15", "A:906877863;C:847589932;G:842258575;T:927440495;N:56527014", 74, 74, null, null, 906877863, 847589932, 842258575, 927440495, 56527014, "SRX6603866", "SRS5168915", "SRA927891", "Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics", "Novartis Institutes for Biomedical Research", 2, 0.94558, 0.95003, 0.09575, 0.09468, 0.72099, 0.72192, 0.52455, 0.51579, 75, 76, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "Germany", "2019-07-27", "Adult", "Adult", "Kidney", "Renal System"], [53377, "SRR9849279", "SRX6603808", "SRS5168857", "SRP216556", "PRJNA556842", "aKlotho regulates age associated vascular calcification and lifespan in zebrafish", "PRJNA556842", "Transcriptome Analysis", "The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast  its overexpression increases lifespan. Given the severe mouse phenotype  we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish  with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body  most dramatically in the outflow tract of the heart  the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.", null, null, null, "wildtype  kidney  5 mpf  replicate 7", "wt 14560 f kidney", null, "strain:AB|age:5months|sex:female|tissue:kidney|genotype:wildtype|Replicate:Bilogical replicate 7|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "wildtype  kidney  5 mpf  replicate 7", "wt 14560 f kidney", "wt 14560 f kidney", "Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills  heart and kidney were collected from 32 individual fish at two time points  namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point   4 males  4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C  and samples were stored at  80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing  mercaptoethanol and stored at  80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP216556", null, null, "wt-14560-f-kidney_merged_S65-1.fastq.gz wt-14560-f-kidney_merged_S65-2.fastq.gz", "fastq fastq", 3381175351.0, 22760562.0, "wt 14560 f kidney merged S65 1.fastq.gz", "0:74.29 1:74.26", "A:834174555;C:827862556;G:819581002;T:856481808;N:43075430", 74, 74, null, null, 834174555, 827862556, 819581002, 856481808, 43075430, "SRX6603808", "SRS5168857", "SRA927891", "Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics", "Novartis Institutes for Biomedical Research", 2, 0.96026, 0.96284, 0.07018, 0.06939, 0.69662, 0.69647, 0.48686, 0.48311, 76, 76, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "Germany", "2019-07-27", "Adult", "Adult", "Kidney", "Renal System"], [53378, "SRR9849280", "SRX6603807", "SRS5168856", "SRP216556", "PRJNA556842", "aKlotho regulates age associated vascular calcification and lifespan in zebrafish", "PRJNA556842", "Transcriptome Analysis", "The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast  its overexpression increases lifespan. Given the severe mouse phenotype  we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish  with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body  most dramatically in the outflow tract of the heart  the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.", null, null, null, "wildtype  kidney  5 mpf  replicate 6", "wt 14560 d kidney", null, "strain:AB|age:5months|sex:female|tissue:kidney|genotype:wildtype|Replicate:Bilogical replicate 6|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "wildtype  kidney  5 mpf  replicate 6", "wt 14560 d kidney", "wt 14560 d kidney", "Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills  heart and kidney were collected from 32 individual fish at two time points  namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point   4 males  4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C  and samples were stored at  80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing  mercaptoethanol and stored at  80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP216556", null, null, "wt-14560-d-kidney_merged_S41-1.fastq.gz wt-14560-d-kidney_merged_S41-2.fastq.gz", "fastq fastq", 3260080878.0, 21943002.0, "wt 14560 d kidney merged S41 1.fastq.gz", "0:74.31 1:74.26", "A:781693169;C:817346676;G:813482706;T:801873503;N:45684824", 74, 74, null, null, 781693169, 817346676, 813482706, 801873503, 45684824, "SRX6603807", "SRS5168856", "SRA927891", "Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics", "Novartis Institutes for Biomedical Research", 2, 0.96382, 0.96558, 0.05582, 0.05496, 0.72245, 0.72342, 0.50059, 0.50428, 76, 76, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "Germany", "2019-07-27", "Adult", "Adult", "Kidney", "Renal System"], [62687, "SRR13319605", "SRX9747096", "SRS7934718", "SRP299583", "PRJNA688352", "Transcriptome Sequencing of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells", "PRJNA688352", "Other", "A transcriptome analysis of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells", null, null, null, "Mut 10d k1", "Mut 10d k1", null, "strain:AB|isolate:missing|breed:missing|cultivar:missing|ecotype:missing|age:15 month|sex:not collected|tissue:kidney and headkidney|biomaterial provider:Southwest University  China|replicate:Biological replicate 1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio: kidney and headkidney from  wild type and congenitally asplenic zebrafish post vaccination", "Mut 10d k1", "Mut 10d k1", "other", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "HiSeq X Ten", null, "SRP299583", null, null, "Mut_10d_k1.raw_1.fastq.gz Mut_10d_k1.raw_2.fastq.gz", "fastq fastq", 6906287100.0, 23020957.0, "Mut 10d k1.raw 1.fastq.gz", "0:150 1:150", "A:1908295029;C:1526919757;G:1627893432;T:1843041112;N:137770", 150, 150, null, null, 1908295029, 1526919757, 1627893432, 1843041112, 137770, "SRX9747096", "SRS7934718", "SRA1178602", "southwest university|college of fisheries", "southwest university", 2, 0.94038, 0.93872, 0.08297, 0.08272, 0.71382, 0.71539, 0.54124, 0.54083, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "other", "unknown", "bulk", "unknown", "unknown", null, "China", "2020-12-28", "Adult", "Adult", "Kidney", "Renal System"], [62688, "SRR13319606", "SRX9747095", "SRS7934719", "SRP299583", "PRJNA688352", "Transcriptome Sequencing of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells", "PRJNA688352", "Other", "A transcriptome analysis of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells", null, null, null, "WT 10d k3", "WT 10d k3", null, "strain:AB|isolate:missing|breed:missing|cultivar:missing|ecotype:missing|age:14 month|sex:not collected|tissue:kidney and headkidney|biomaterial provider:Southwest University  China|replicate:Biological replicate 3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio: kidney and headkidney from  wild type and congenitally asplenic zebrafish post vaccination", "WT 10d k3", "WT 10d k3", "other", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "HiSeq X Ten", null, "SRP299583", null, null, "WT_10d_k3.raw_1.fastq.gz WT_10d_k3.raw_2.fastq.gz", "fastq fastq", 7159590600.0, 23865302.0, "WT 10d k3.raw 1.fastq.gz", "0:150 1:150", "A:1984034638;C:1574065265;G:1674336416;T:1927009990;N:144291", 150, 150, null, null, 1984034638, 1574065265, 1674336416, 1927009990, 144291, "SRX9747095", "SRS7934719", "SRA1178602", "southwest university|college of fisheries", "southwest university", 2, 0.92946, 0.92803, 0.08386, 0.08381, 0.71052, 0.71074, 0.51146, 0.51825, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "other", "unknown", "bulk", "unknown", "unknown", null, "China", "2020-12-28", "Adult", "Adult", "Kidney", "Renal System"], [62689, "SRR13319607", "SRX9747094", "SRS7934714", "SRP299583", "PRJNA688352", "Transcriptome Sequencing of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells", "PRJNA688352", "Other", "A transcriptome analysis of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells", null, null, null, "WT 10d k2", "WT 10d k2", null, "strain:AB|isolate:missing|breed:missing|cultivar:missing|ecotype:missing|age:13 month|sex:not collected|tissue:kidney and headkidney|biomaterial provider:Southwest University  China|replicate:Biological replicate 2|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio: kidney and headkidney from  wild type and congenitally asplenic zebrafish post vaccination", "WT 10d k2", "WT 10d k2", "other", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "HiSeq X Ten", null, "SRP299583", null, null, "WT_10d_k2.raw_1.fastq.gz WT_10d_k2.raw_2.fastq.gz", "fastq fastq", 6354585300.0, 21181951.0, "WT 10d k2.raw 1.fastq.gz", "0:150 1:150", "A:1749380892;C:1406921465;G:1504331323;T:1693821680;N:129940", 150, 150, null, null, 1749380892, 1406921465, 1504331323, 1693821680, 129940, "SRX9747094", "SRS7934714", "SRA1178602", "southwest university|college of fisheries", "southwest university", 2, 0.93216, 0.9306, 0.0766, 0.07651, 0.70847, 0.70936, 0.53878, 0.53632, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "other", "unknown", "bulk", "unknown", "unknown", null, "China", "2020-12-28", "Adult", "Adult", "Kidney", "Renal System"], [62690, "SRR13319608", "SRX9747093", "SRS7934720", "SRP299583", "PRJNA688352", "Transcriptome Sequencing of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells", "PRJNA688352", "Other", "A transcriptome analysis of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells", null, null, null, "WT 10d k1", "WT 10d k1", null, "strain:AB|isolate:missing|breed:missing|cultivar:missing|ecotype:missing|age:12 month|sex:not collected|tissue:kidney and headkidney|biomaterial provider:Southwest University  China|replicate:Biological replicate 1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio: kidney and headkidney from  wild type and congenitally asplenic zebrafish post vaccination", "WT 10d k1", "WT 10d k1", "other", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "HiSeq X Ten", null, "SRP299583", null, null, "WT_10d_k1.raw_1.fastq.gz WT_10d_k1.raw_2.fastq.gz", "fastq fastq", 8712565800.0, 29041886.0, "WT 10d k1.raw 1.fastq.gz", "0:150 1:150", "A:2388117969;C:1934065753;G:2091791049;T:2298416148;N:174881", 150, 150, null, null, 2388117969, 1934065753, 2091791049, 2298416148, 174881, "SRX9747093", "SRS7934720", "SRA1178602", "southwest university|college of fisheries", "southwest university", 2, 0.93468, 0.93398, 0.09783, 0.0975, 0.69593, 0.69649, 0.52524, 0.52599, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "other", "unknown", "bulk", "unknown", "unknown", null, "China", "2020-12-28", "Adult", "Adult", "Kidney", "Renal System"], [62691, "SRR13319609", "SRX9747092", "SRS7934712", "SRP299583", "PRJNA688352", "Transcriptome Sequencing of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells", "PRJNA688352", "Other", "A transcriptome analysis of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells", null, null, null, "Mut 6h k3", "Mut 6h k3", null, "strain:AB|isolate:missing|breed:missing|cultivar:missing|ecotype:missing|age:11 month|sex:not collected|tissue:kidney and headkidney|biomaterial provider:Southwest University  China|replicate:Biological replicate 3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio: kidney and headkidney from  wild type and congenitally asplenic zebrafish post vaccination", "Mut 6h k3", "Mut 6h k3", "other", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "HiSeq X Ten", null, "SRP299583", null, null, "Mut_6h_k3.raw_1.fastq.gz Mut_6h_k3.raw_2.fastq.gz", "fastq fastq", 6821167500.0, 22737225.0, "Mut 6h k3.raw 1.fastq.gz", "0:150 1:150", "A:1903976437;C:1488858303;G:1566082857;T:1862227563;N:22340", 150, 150, null, null, 1903976437, 1488858303, 1566082857, 1862227563, 22340, "SRX9747092", "SRS7934712", "SRA1178602", "southwest university|college of fisheries", "southwest university", 2, 0.93422, 0.93209, 0.09882, 0.09902, 0.71784, 0.71892, 0.53469, 0.5464, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "other", "unknown", "bulk", "unknown", "unknown", null, "China", "2020-12-28", "Adult", "Adult", "Kidney", "Renal System"], [62692, "SRR13319610", "SRX9747091", "SRS7934717", "SRP299583", "PRJNA688352", "Transcriptome Sequencing of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells", "PRJNA688352", "Other", "A transcriptome analysis of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells", null, null, null, "Mut 6h k2", "Mut 6h k2", null, "strain:AB|isolate:missing|breed:missing|cultivar:missing|ecotype:missing|age:10 month|sex:not collected|tissue:kidney and headkidney|biomaterial provider:Southwest University  China|replicate:Biological replicate 2|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio: kidney and headkidney from  wild type and congenitally asplenic zebrafish post vaccination", "Mut 6h k2", "Mut 6h k2", "other", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "HiSeq X Ten", null, "SRP299583", null, null, "Mut_6h_k2.raw_1.fastq.gz Mut_6h_k2.raw_2.fastq.gz", "fastq fastq", 6614007900.0, 22046693.0, "Mut 6h k2.raw 1.fastq.gz", "0:150 1:150", "A:1836116506;C:1454221417;G:1524536821;T:1799111318;N:21838", 150, 150, null, null, 1836116506, 1454221417, 1524536821, 1799111318, 21838, "SRX9747091", "SRS7934717", "SRA1178602", "southwest university|college of fisheries", "southwest university", 2, 0.93405, 0.93165, 0.09928, 0.0998, 0.71378, 0.71405, 0.53405, 0.53636, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "other", "unknown", "bulk", "unknown", "unknown", null, "China", "2020-12-28", "Adult", "Adult", "Kidney", "Renal System"], [62693, "SRR13319611", "SRX9747090", "SRS7934716", "SRP299583", "PRJNA688352", "Transcriptome Sequencing of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells", "PRJNA688352", "Other", "A transcriptome analysis of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells", null, null, null, "Mut 6h k1", "Mut 6h k1", null, "strain:AB|isolate:missing|breed:missing|cultivar:missing|ecotype:missing|age:9 month|sex:not collected|tissue:kidney and headkidney|biomaterial provider:Southwest University  China|replicate:Biological replicate 1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio: kidney and headkidney from  wild type and congenitally asplenic zebrafish post vaccination", "Mut 6h k1", "Mut 6h k1", "other", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "HiSeq X Ten", null, "SRP299583", null, null, "Mut_6h_k1.raw_1.fastq.gz Mut_6h_k1.raw_2.fastq.gz", "fastq fastq", 6856096500.0, 22853655.0, "Mut 6h k1.raw 1.fastq.gz", "0:150 1:150", "A:1914687245;C:1494001561;G:1567738680;T:1879647930;N:21084", 150, 150, null, null, 1914687245, 1494001561, 1567738680, 1879647930, 21084, "SRX9747090", "SRS7934716", "SRA1178602", "southwest university|college of fisheries", "southwest university", 2, 0.92882, 0.92611, 0.10791, 0.10832, 0.71378, 0.71427, 0.53012, 0.52707, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "other", "unknown", "bulk", "unknown", "unknown", null, "China", "2020-12-28", "Adult", "Adult", "Kidney", "Renal System"], [62694, "SRR13319612", "SRX9747089", "SRS7934713", "SRP299583", "PRJNA688352", "Transcriptome Sequencing of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells", "PRJNA688352", "Other", "A transcriptome analysis of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells", null, null, null, "WT 6h k3", "WT 6h k3", null, "strain:AB|isolate:missing|breed:missing|cultivar:missing|ecotype:missing|age:8 month|sex:not collected|tissue:kidney and headkidney|biomaterial provider:Southwest University  China|replicate:Biological replicate 3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio: kidney and headkidney from  wild type and congenitally asplenic zebrafish post vaccination", "WT 6h k3", "WT 6h k3", "other", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "HiSeq X Ten", null, "SRP299583", null, null, "WT_6h_k3.raw_1.fastq.gz WT_6h_k3.raw_2.fastq.gz", "fastq fastq", 7194856500.0, 23982855.0, "WT 6h k3.raw 1.fastq.gz", "0:150 1:150", "A:2000223263;C:1580258499;G:1657803759;T:1956549208;N:21771", 150, 150, null, null, 2000223263, 1580258499, 1657803759, 1956549208, 21771, "SRX9747089", "SRS7934713", "SRA1178602", "southwest university|college of fisheries", "southwest university", 2, 0.93193, 0.92986, 0.09623, 0.09609, 0.70907, 0.71029, 0.53843, 0.53754, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "other", "unknown", "bulk", "unknown", "unknown", null, "China", "2020-12-28", "Adult", "Adult", "Kidney", "Renal System"], [62695, "SRR13319613", "SRX9747088", "SRS7934715", "SRP299583", "PRJNA688352", "Transcriptome Sequencing of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells", "PRJNA688352", "Other", "A transcriptome analysis of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells", null, null, null, "Mut 10d k3", "Mut 10d k3", null, "strain:AB|isolate:missing|breed:missing|cultivar:missing|ecotype:missing|age:17 month|sex:not collected|tissue:kidney and headkidney|biomaterial provider:Southwest University  China|replicate:Biological replicate 3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio: kidney and headkidney from  wild type and congenitally asplenic zebrafish post vaccination", "Mut 10d k3", "Mut 10d k3", "other", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "HiSeq X Ten", null, "SRP299583", null, null, "Mut_10d_k3.raw_1.fastq.gz Mut_10d_k3.raw_2.fastq.gz", "fastq fastq", 7859046900.0, 26196823.0, "Mut 10d k3.raw 1.fastq.gz", "0:150 1:150", "A:2162835249;C:1743162639;G:1857995098;T:2094896175;N:157739", 150, 150, null, null, 2162835249, 1743162639, 1857995098, 2094896175, 157739, "SRX9747088", "SRS7934715", "SRA1178602", "southwest university|college of fisheries", "southwest university", 2, 0.93831, 0.93732, 0.0836, 0.08331, 0.69988, 0.70132, 0.54542, 0.54383, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "other", "unknown", "bulk", "unknown", "unknown", null, "China", "2020-12-28", "Adult", "Adult", "Kidney", "Renal System"], [62696, "SRR13319614", "SRX9747087", "SRS7934710", "SRP299583", "PRJNA688352", "Transcriptome Sequencing of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells", "PRJNA688352", "Other", "A transcriptome analysis of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells", null, null, null, "Mut 10d k2", "Mut 10d k2", null, "strain:AB|isolate:missing|breed:missing|cultivar:missing|ecotype:missing|age:16 month|sex:not collected|tissue:kidney and headkidney|biomaterial provider:Southwest University  China|replicate:Biological replicate 2|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio: kidney and headkidney from  wild type and congenitally asplenic zebrafish post vaccination", "Mut 10d k2", "Mut 10d k2", "other", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "HiSeq X Ten", null, "SRP299583", null, null, "Mut_10d_k2.raw_1.fastq.gz Mut_10d_k2.raw_2.fastq.gz", "fastq fastq", 6163552500.0, 20545175.0, "Mut 10d k2.raw 1.fastq.gz", "0:150 1:150", "A:1690284188;C:1371212067;G:1471613432;T:1630318376;N:124437", 150, 150, null, null, 1690284188, 1371212067, 1471613432, 1630318376, 124437, "SRX9747087", "SRS7934710", "SRA1178602", "southwest university|college of fisheries", "southwest university", 2, 0.94142, 0.93953, 0.07637, 0.07599, 0.7123, 0.71323, 0.54883, 0.55044, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "other", "unknown", "bulk", "unknown", "unknown", null, "China", "2020-12-28", "Adult", "Adult", "Kidney", "Renal System"], [62697, "SRR13319615", "SRX9747086", "SRS7934709", "SRP299583", "PRJNA688352", "Transcriptome Sequencing of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells", "PRJNA688352", "Other", "A transcriptome analysis of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells", null, null, null, "WT 6h k2", "WT 6h k2", null, "strain:AB|isolate:missing|breed:missing|cultivar:missing|ecotype:missing|age:7 month|sex:not collected|tissue:kidney and headkidney|biomaterial provider:Southwest University  China|replicate:Biological replicate 2|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio: kidney and headkidney from  wild type and congenitally asplenic zebrafish post vaccination", "WT 6h k2", "WT 6h k2", "other", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "HiSeq X Ten", null, "SRP299583", null, null, "WT_6h_k2.raw_1.fastq.gz WT_6h_k2.raw_2.fastq.gz", "fastq fastq", 7675977600.0, 25586592.0, "WT 6h k2.raw 1.fastq.gz", "0:150 1:150", "A:2123159765;C:1695880551;G:1771133441;T:2085779945;N:23898", 150, 150, null, null, 2123159765, 1695880551, 1771133441, 2085779945, 23898, "SRX9747086", "SRS7934709", "SRA1178602", "southwest university|college of fisheries", "southwest university", 2, 0.93307, 0.93101, 0.09081, 0.09046, 0.7097, 0.71031, 0.52694, 0.52787, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "other", "unknown", "bulk", "unknown", "unknown", null, "China", "2020-12-28", "Adult", "Adult", "Kidney", "Renal System"], [62698, "SRR13319616", "SRX9747085", "SRS7934711", "SRP299583", "PRJNA688352", "Transcriptome Sequencing of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells", "PRJNA688352", "Other", "A transcriptome analysis of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells", null, null, null, "WT 6h k1", "WT 6h k1", null, "strain:AB|isolate:missing|breed:missing|cultivar:missing|ecotype:missing|age:6 month|sex:not collected|tissue:kidney and headkidney|biomaterial provider:Southwest University  China|replicate:Biological replicate 1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio: kidney and headkidney from  wild type and congenitally asplenic zebrafish post vaccination", "WT 6h k1", "WT 6h k1", "other", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "HiSeq X Ten", null, "SRP299583", null, null, "WT_6h_k1.raw_1.fastq.gz WT_6h_k1.raw_2.fastq.gz", "fastq fastq", 5660758200.0, 18869194.0, "WT 6h k1.raw 1.fastq.gz", "0:150 1:150", "A:1560962707;C:1253335257;G:1319974501;T:1526464986;N:20749", 150, 150, null, null, 1560962707, 1253335257, 1319974501, 1526464986, 20749, "SRX9747085", "SRS7934711", "SRA1178602", "southwest university|college of fisheries", "southwest university", 2, 0.93079, 0.92974, 0.09975, 0.09988, 0.70504, 0.70581, 0.53219, 0.533, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "other", "unknown", "bulk", "unknown", "unknown", null, "China", "2020-12-28", "Adult", "Adult", "Kidney", "Renal System"], [62700, "SRR13327930", "SRX9755046", "SRS7944756", "SRP299775", "PRJNA688655", "RNA Seq of Danio rerio: kidney and headkidney from wild type and congenitally asplenic zebrafish", "PRJNA688655", "Other", null, null, null, null, "Mut 0h k3", "Mut 0h k3", null, "strain:AB|isolate:missing|breed:missing|cultivar:missing|ecotype:missing|age:11|dev stage:mission|sex:not collected|tissue:kidney and headkidney|biomaterial provider:Southwest University  China|replicate:Biological replicate 3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio: kidney and headkidney from  wild type and congenitally asplenic zebrafish", "Mut 0h k3", "Mut k3", "other", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "HiSeq X Ten", null, "SRP299775", null, null, "WT_0h_k3.raw_1.fastq.gz WT_0h_k3.raw_2.fastq.gz", "fastq fastq", 6675348000.0, 22251160.0, "WT 0h k3.raw 1.fastq.gz", "0:150 1:150", "A:1848988411;C:1470625677;G:1544465916;T:1811246237;N:21759", 150, 150, null, null, 1848988411, 1470625677, 1544465916, 1811246237, 21759, "SRX9755046", "SRS7944756", "SRA1178968", "southwest university|college of fisheries", "southwest university", 2, 0.86263, 0.92706, 0.08978, 0.0978, 0.70502, 0.69895, 0.55273, 0.55505, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "other", "unknown", "bulk", "unknown", "unknown", null, "China", "2020-12-30", "Undetermined", "Undetermined", "Kidney", "Renal System"], [62701, "SRR13327931", "SRX9755045", "SRS7944755", "SRP299775", "PRJNA688655", "RNA Seq of Danio rerio: kidney and headkidney from wild type and congenitally asplenic zebrafish", "PRJNA688655", "Other", null, null, null, null, "Mut 0h k2", "Mut 0h k2", null, "strain:AB|isolate:missing|breed:missing|cultivar:missing|ecotype:missing|age:10|dev stage:mission|sex:not collected|tissue:kidney and headkidney|biomaterial provider:Southwest University  China|replicate:Biological replicate 2|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio: kidney and headkidney from  wild type and congenitally asplenic zebrafish", "Mut 0h k2", "Mut k2", "other", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "HiSeq X Ten", null, "SRP299775", null, null, "WT_0h_k2.raw_1.fastq.gz WT_0h_k2.raw_2.fastq.gz", "fastq fastq", 7165537800.0, 23885126.0, "WT 0h k2.raw 1.fastq.gz", "0:150 1:150", "A:1961366053;C:1599856726;G:1683336568;T:1920956264;N:22189", 150, 150, null, null, 1961366053, 1599856726, 1683336568, 1920956264, 22189, "SRX9755045", "SRS7944755", "SRA1178968", "southwest university|college of fisheries", "southwest university", 2, 0.93437, 0.93173, 0.09166, 0.09117, 0.6997, 0.70031, 0.5364, 0.53886, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "other", "unknown", "bulk", "unknown", "unknown", null, "China", "2020-12-30", "Undetermined", "Undetermined", "Kidney", "Renal System"], [62702, "SRR13327932", "SRX9755044", "SRS7944754", "SRP299775", "PRJNA688655", "RNA Seq of Danio rerio: kidney and headkidney from wild type and congenitally asplenic zebrafish", "PRJNA688655", "Other", null, null, null, null, "Mut 0h k1", "Mut 0h k1", null, "strain:AB|isolate:missing|breed:missing|cultivar:missing|ecotype:missing|age:9|dev stage:mission|sex:not collected|tissue:kidney and headkidney|biomaterial provider:Southwest University  China|replicate:Biological replicate 1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio: kidney and headkidney from  wild type and congenitally asplenic zebrafish", "Mut 0h k1", "Mut k1", "other", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "HiSeq X Ten", null, "SRP299775", null, null, "WT_0h_k1.raw_1.fastq.gz WT_0h_k1.raw_2.fastq.gz", "fastq fastq", 5953238400.0, 19844128.0, "WT 0h k1.raw 1.fastq.gz", "0:150 1:150", "A:1643432335;C:1323053555;G:1377906382;T:1608824934;N:21194", 150, 150, null, null, 1643432335, 1323053555, 1377906382, 1608824934, 21194, "SRX9755044", "SRS7944754", "SRA1178968", "southwest university|college of fisheries", "southwest university", 2, 0.93408, 0.93293, 0.08519, 0.08534, 0.70952, 0.71029, 0.56062, 0.52712, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "other", "unknown", "bulk", "unknown", "unknown", null, "China", "2020-12-30", "Undetermined", "Undetermined", "Kidney", "Renal System"], [62703, "SRR13327933", "SRX9755043", "SRS7944753", "SRP299775", "PRJNA688655", "RNA Seq of Danio rerio: kidney and headkidney from wild type and congenitally asplenic zebrafish", "PRJNA688655", "Other", null, null, null, null, "WT 0h k3", "WT 0h k3", null, "strain:AB|isolate:missing|breed:missing|cultivar:missing|ecotype:missing|age:8|dev stage:mission|sex:not collected|tissue:kidney and headkidney|biomaterial provider:Southwest University  China|replicate:Biological replicate 3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio: kidney and headkidney from  wild type and congenitally asplenic zebrafish", "WT 0h k3", "WT k3", "other", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "HiSeq X Ten", null, "SRP299775", null, null, "Mut_0h_k3.raw_1.fastq.gz Mut_0h_k3.raw_2.fastq.gz", "fastq fastq", 5891495100.0, 19638317.0, "Mut 0h k3.raw 1.fastq.gz", "0:150 1:150", "A:1626936306;C:1303639063;G:1370950045;T:1589948552;N:21134", 150, 150, null, null, 1626936306, 1303639063, 1370950045, 1589948552, 21134, "SRX9755043", "SRS7944753", "SRA1178968", "southwest university|college of fisheries", "southwest university", 2, 0.93117, 0.93006, 0.09742, 0.09724, 0.70396, 0.7041, 0.53898, 0.53936, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "other", "unknown", "bulk", "unknown", "unknown", null, "China", "2020-12-30", "Undetermined", "Undetermined", "Kidney", "Renal System"], [62704, "SRR13327934", "SRX9755042", "SRS7944752", "SRP299775", "PRJNA688655", "RNA Seq of Danio rerio: kidney and headkidney from wild type and congenitally asplenic zebrafish", "PRJNA688655", "Other", null, null, null, null, "WT 0h k2", "WT 0h k2", null, "strain:AB|isolate:missing|breed:missing|cultivar:missing|ecotype:missing|age:7|dev stage:mission|sex:not collected|tissue:kidney and headkidney|biomaterial provider:Southwest University  China|replicate:Biological replicate 2|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio: kidney and headkidney from  wild type and congenitally asplenic zebrafish", "WT 0h k2", "WT k2", "other", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "HiSeq X Ten", null, "SRP299775", null, null, "Mut_0h_k2.raw_1.fastq.gz Mut_0h_k2.raw_2.fastq.gz", "fastq fastq", 5543211000.0, 18477370.0, "Mut 0h k2.raw 1.fastq.gz", "0:150 1:150", "A:1531314014;C:1225009325;G:1291427420;T:1495440150;N:20091", 150, 150, null, null, 1531314014, 1225009325, 1291427420, 1495440150, 20091, "SRX9755042", "SRS7944752", "SRA1178968", "southwest university|college of fisheries", "southwest university", 2, 0.93219, 0.93172, 0.10466, 0.10531, 0.70141, 0.70104, 0.53257, 0.53162, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "other", "unknown", "bulk", "unknown", "unknown", null, "China", "2020-12-30", "Undetermined", "Undetermined", "Kidney", "Renal System"], [62705, "SRR13327935", "SRX9755041", "SRS7944751", "SRP299775", "PRJNA688655", "RNA Seq of Danio rerio: kidney and headkidney from wild type and congenitally asplenic zebrafish", "PRJNA688655", "Other", null, null, null, null, "WT 0h k1", "WT 0h k1", null, "strain:AB|isolate:missing|breed:missing|cultivar:missing|ecotype:missing|age:6|dev stage:mission|sex:not collected|tissue:kidney and headkidney|biomaterial provider:Southwest University  China|replicate:Biological replicate 1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio: kidney and headkidney from  wild type and congenitally asplenic zebrafish", "WT 0h k1", "WT k1", "other", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "HiSeq X Ten", null, "SRP299775", null, null, "Mut_0h_k1.raw_1.fastq.gz Mut_0h_k1.raw_2.fastq.gz", "fastq fastq", 6954804600.0, 23182682.0, "Mut 0h k1.raw 1.fastq.gz", "0:150 1:150", "A:1929335607;C:1531367791;G:1605966700;T:1888112325;N:22177", 150, 150, null, null, 1929335607, 1531367791, 1605966700, 1888112325, 22177, "SRX9755041", "SRS7944751", "SRA1178968", "southwest university|college of fisheries", "southwest university", 2, 0.9401, 0.93841, 0.08725, 0.08725, 0.71536, 0.71569, 0.56381, 0.56397, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "other", "unknown", "bulk", "unknown", "unknown", null, "China", "2020-12-30", "Undetermined", "Undetermined", "Kidney", "Renal System"], [72561, "SRR22722190", "SRX18683720", "SRS16126888", "SRP412911", "PRJNA911847", "Transgenic IDH2 R172K and IDH2 R140Q zebrafish models recapitulated features of human acute myeloid leukaemia", "PRJNA911847", "Other", "Isocitrate dehydrogenase 2 IDH2 mutations occur in more than 15% of cytogenetically normal acute myeloid leukemia CN AML but comparative studies of their roles in leukemogenesis have been scarce. We generated zebrafish models of IDH2R172K and IDH2R140Q AML and reported their pathologic  functional and transcriptomic features and therapeutic responses to target therapies. Transgenic embryos co expressing FLT3ITD and IDH2 mutations showed accentuation of myelopoiesis. As these embryos were raised to maturity  full blown leukemia ensued with multi lineage dysplasia  increase in myeloblasts and marrow cellularity and splenomegaly. The leukemia cells were transplantable into primary and secondary recipients and resulted in more aggressive disease. TgRunx1:FLT3ITDIDH2R172K but not TgRunx1:FLT3ITDIDH2R140Q zebrafish showed increase in T cell development at embryonic and adult stage. Single cell transcriptomic analysis revealed increased myeloid skewing  differentiation blockade and enrichment of leukemia associated gene signatures in both zebrafish models. TgRunx1:FLT3ITDIDH2R172K but not TgRunx1:FLT3ITDIDH2R140Q zebrafish showed increase in interferon signals at adult stage. Leukemic phenotypes in both zebrafish could be ameliorated by quizartinib and enasidenib. In conclusion  the zebrafish models of IDH2 mutated AML recapitulated the morphologic  clinical  functional and transcriptomic characteristics of human diseases  and provided the prototype for developing zebrafish leukemia models of other genotypes that would become a platform for high throughput drug screening", null, null, null, null, "Transgenic FLT3 ITD IDH2 R140Q", null, "strain:TU|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:8 month|dev stage:8 month|sex:pooled male and female|tissue:kidney marrow|genotype:Transgenic Runx1:FLT3ITDIDH2R140Q|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Single cell RNA seq of Danio rerio: Transgenic FLT3 ITD IDH2 R140Q", "ITD140Q", "ITD140Q", "Single cell RNA seq library.Single viable KM cells were collected from the transgenic double mutant n=3; pooled and WT n=3; pooled fish at 7 mpf into 0.9X PBS with 5% FBS. Their viability was examined by 0.4% Trypan blue staining under microscopy. The single cell library was constructed using the ChromiumTM Controller and ChromiumTM Next GEM Single Cell 3 Kit v3.1 10x Genomics  Pleasanton  CA. Complementary DNA cDNA was synthesized from the fragmentated RNAs using N6 random primers  followed by end repair and ligation to BGISEQ sequencer compatible adapters. Quality control of the final library was performed by checking the distribution of the fragments size using the Agilent 2100 bioanalyzer and quantification was performed by real time quantitative PCR using TaqMan probes. The final products were sequenced using the DNBSEQTM platform BGI  HK  China.", null, null, "OTHER", "TRANSCRIPTOMIC", "other", "PAIRED", "DNBSEQ", "DNBSEQ-G400", null, "SRP412911", null, "loader:fastq load.py", "ITD172KA_S1_L001_I1_001.fastq.gz ITD172KA_S1_L001_R1_001.fastq.gz ITD172KA_S1_L001_R2_001.fastq.gz", "fastq fastq fastq", 52149830422.0, 410628586.0, "ITD172KA S1 L001 I1 001.fastq.gz", "0:8 1:28 2:91", "A:10282953217;C:8459559201;G:8851285287;T:9772343870;N:1059751", 8, 28, 91, null, 10282953217, 8459559201, 8851285287, 9772343870, 1059751, "SRX18683720", "SRS16126888", "SRA1558824", "The University of Hong Kong|Department of Medicine", "The University of Hong Kong", 1, 0.88465, null, 0.13112, null, 0.81789, null, 0.55842, null, 91, null, "B", null, "usable mapping rate", "bgi", "bgi", "unknown", "random_priming", "unknown", "sc", "single_cell_droplet", "10x", null, "China", "2022-12-14", "Adult", "Adult", "Kidney", "Renal System"], [72562, "SRR22722191", "SRX18683719", "SRS16126887", "SRP412911", "PRJNA911847", "Transgenic IDH2 R172K and IDH2 R140Q zebrafish models recapitulated features of human acute myeloid leukaemia", "PRJNA911847", "Other", "Isocitrate dehydrogenase 2 IDH2 mutations occur in more than 15% of cytogenetically normal acute myeloid leukemia CN AML but comparative studies of their roles in leukemogenesis have been scarce. We generated zebrafish models of IDH2R172K and IDH2R140Q AML and reported their pathologic  functional and transcriptomic features and therapeutic responses to target therapies. Transgenic embryos co expressing FLT3ITD and IDH2 mutations showed accentuation of myelopoiesis. As these embryos were raised to maturity  full blown leukemia ensued with multi lineage dysplasia  increase in myeloblasts and marrow cellularity and splenomegaly. The leukemia cells were transplantable into primary and secondary recipients and resulted in more aggressive disease. TgRunx1:FLT3ITDIDH2R172K but not TgRunx1:FLT3ITDIDH2R140Q zebrafish showed increase in T cell development at embryonic and adult stage. Single cell transcriptomic analysis revealed increased myeloid skewing  differentiation blockade and enrichment of leukemia associated gene signatures in both zebrafish models. TgRunx1:FLT3ITDIDH2R172K but not TgRunx1:FLT3ITDIDH2R140Q zebrafish showed increase in interferon signals at adult stage. Leukemic phenotypes in both zebrafish could be ameliorated by quizartinib and enasidenib. In conclusion  the zebrafish models of IDH2 mutated AML recapitulated the morphologic  clinical  functional and transcriptomic characteristics of human diseases  and provided the prototype for developing zebrafish leukemia models of other genotypes that would become a platform for high throughput drug screening", null, null, null, null, "Transgenic FLT3 ITD IDH2 R172K", null, "strain:TU|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:8 month|dev stage:8 month|sex:pooled male and female|tissue:kidney marrow|genotype:Transgenic Runx1:FLT3ITDIDH2R172K|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Single cell RNA seq of Danio rerio: Transgenic FLT3 ITD IDH2 R172K", "ITD172K", "ITD172K", "Single cell RNA seq library.Single viable KM cells were collected from the transgenic double mutant n=3; pooled and WT n=3; pooled fish at 7 mpf into 0.9X PBS with 5% FBS. Their viability was examined by 0.4% Trypan blue staining under microscopy. The single cell library was constructed using the ChromiumTM Controller and ChromiumTM Next GEM Single Cell 3 Kit v3.1 10x Genomics  Pleasanton  CA. Complementary DNA cDNA was synthesized from the fragmentated RNAs using N6 random primers  followed by end repair and ligation to BGISEQ sequencer compatible adapters. Quality control of the final library was performed by checking the distribution of the fragments size using the Agilent 2100 bioanalyzer and quantification was performed by real time quantitative PCR using TaqMan probes. The final products were sequenced using the DNBSEQTM platform BGI  HK  China.", null, null, "OTHER", "TRANSCRIPTOMIC", "other", "PAIRED", "DNBSEQ", "DNBSEQ-G400", null, "SRP412911", null, "loader:fastq load.py", "ITD140QA_S1_L004_I1_001.fastq.gz ITD140QA_S1_L004_R1_001.fastq.gz ITD140QA_S1_L004_R2_001.fastq.gz", "fastq fastq fastq", 28595025233.0, 225157679.0, "ITD140QA S1 L004 I1 001.fastq.gz", "0:8 1:28 2:91", "A:5628987577;C:4565848641;G:4959209904;T:5302394659;N:32908008", 8, 28, 91, null, 5628987577, 4565848641, 4959209904, 5302394659, 32908008, "SRX18683719", "SRS16126887", "SRA1558824", "The University of Hong Kong|Department of Medicine", "The University of Hong Kong", 1, 0.81404, null, 0.11315, null, 0.83327, null, 0.56222, null, 91, null, "B", null, "usable mapping rate", "bgi", "bgi", "unknown", "random_priming", "unknown", "sc", "single_cell_droplet", "10x", null, "China", "2022-12-13", "Adult", "Adult", "Kidney", "Renal System"], [72563, "SRR22722192", "SRX18683718", "SRS16126886", "SRP412911", "PRJNA911847", "Transgenic IDH2 R172K and IDH2 R140Q zebrafish models recapitulated features of human acute myeloid leukaemia", "PRJNA911847", "Other", "Isocitrate dehydrogenase 2 IDH2 mutations occur in more than 15% of cytogenetically normal acute myeloid leukemia CN AML but comparative studies of their roles in leukemogenesis have been scarce. We generated zebrafish models of IDH2R172K and IDH2R140Q AML and reported their pathologic  functional and transcriptomic features and therapeutic responses to target therapies. Transgenic embryos co expressing FLT3ITD and IDH2 mutations showed accentuation of myelopoiesis. As these embryos were raised to maturity  full blown leukemia ensued with multi lineage dysplasia  increase in myeloblasts and marrow cellularity and splenomegaly. The leukemia cells were transplantable into primary and secondary recipients and resulted in more aggressive disease. TgRunx1:FLT3ITDIDH2R172K but not TgRunx1:FLT3ITDIDH2R140Q zebrafish showed increase in T cell development at embryonic and adult stage. Single cell transcriptomic analysis revealed increased myeloid skewing  differentiation blockade and enrichment of leukemia associated gene signatures in both zebrafish models. TgRunx1:FLT3ITDIDH2R172K but not TgRunx1:FLT3ITDIDH2R140Q zebrafish showed increase in interferon signals at adult stage. Leukemic phenotypes in both zebrafish could be ameliorated by quizartinib and enasidenib. In conclusion  the zebrafish models of IDH2 mutated AML recapitulated the morphologic  clinical  functional and transcriptomic characteristics of human diseases  and provided the prototype for developing zebrafish leukemia models of other genotypes that would become a platform for high throughput drug screening", null, null, null, null, "Wild type", null, "strain:TU|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:8 month|dev stage:8 month|sex:pooled male and female|tissue:kidney marrow|genotype:Wild type|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Single cell RNA seq of Danio rerio: Wild type", "Control", "Control", "Single cell RNA seq library.Single viable KM cells were collected from the transgenic double mutant n=3; pooled and WT n=3; pooled fish at 7 mpf into 0.9X PBS with 5% FBS. Their viability was examined by 0.4% Trypan blue staining under microscopy. The single cell library was constructed using the ChromiumTM Controller and ChromiumTM Next GEM Single Cell 3 Kit v3.1 10x Genomics  Pleasanton  CA. Complementary DNA cDNA was synthesized from the fragmentated RNAs using N6 random primers  followed by end repair and ligation to BGISEQ sequencer compatible adapters. Quality control of the final library was performed by checking the distribution of the fragments size using the Agilent 2100 bioanalyzer and quantification was performed by real time quantitative PCR using TaqMan probes. The final products were sequenced using the DNBSEQTM platform BGI  HK  China.", null, null, "OTHER", "TRANSCRIPTOMIC", "other", "PAIRED", "DNBSEQ", "DNBSEQ-G400", null, "SRP412911", null, "loader:fastq load.py", "ControlA_S1_L003_R2_001.fastq.gz ControlA_S1_L003_R1_001.fastq.gz ControlA_S1_L003_I1_001.fastq.gz", "fastq fastq fastq", 37892727491.0, 298367933.0, "ControlA S1 L003 I1 001.fastq.gz", "0:8 1:28 2:91", "A:7386602298;C:6174680460;G:6579652828;T:6955791213;N:54755104", 8, 28, 91, null, 7386602298, 6174680460, 6579652828, 6955791213, 54755104, "SRX18683718", "SRS16126886", "SRA1558824", "The University of Hong Kong|Department of Medicine", "The University of Hong Kong", 1, 0.81686, null, 0.11049, null, 0.84139, null, 0.46084, null, 91, null, "B", null, "usable mapping rate", "bgi", "bgi", "unknown", "random_priming", "unknown", "sc", "single_cell_droplet", "10x", null, "China", "2022-12-14", "Adult", "Adult", "Kidney", "Renal System"]], "truncated": false, 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