{"database": "metadata", "table": "run_metadata", "is_view": false, "human_description_en": "where experiment.library_selection = \"other\", experiment.library_strategy = \"RNA-Seq\" and tissue_curation_coarse = \"Renal System\"", "rows": [[53291, "SRR9849281", "SRX6603894", "SRS5168943", "SRP216556", "PRJNA556842", "aKlotho regulates age associated vascular calcification and lifespan in zebrafish", "PRJNA556842", "Transcriptome Analysis", "The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast  its overexpression increases lifespan. Given the severe mouse phenotype  we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish  with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body  most dramatically in the outflow tract of the heart  the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.", null, null, null, "wildtype  kidney  5 mpf  replicate 5", "wt 14560 b kidney", null, "strain:AB|age:5months|sex:female|tissue:kidney|genotype:wildtype|Replicate:Bilogical replicate 5|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "wildtype  kidney  5 mpf  replicate 5", "wt 14560 b kidney", "wt 14560 b kidney", "Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills  heart and kidney were collected from 32 individual fish at two time points  namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point   4 males  4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C  and samples were stored at  80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing  mercaptoethanol and stored at  80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. 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RNALater was removed post an overnight incubation at 4C  and samples were stored at  80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing  mercaptoethanol and stored at  80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. 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In contrast  its overexpression increases lifespan. Given the severe mouse phenotype  we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish  with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body  most dramatically in the outflow tract of the heart  the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. 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RNALater was removed post an overnight incubation at 4C  and samples were stored at  80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing  mercaptoethanol and stored at  80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. 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In contrast  its overexpression increases lifespan. Given the severe mouse phenotype  we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish  with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body  most dramatically in the outflow tract of the heart  the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.", null, null, null, "\u03b1Klotho  kidney  3 mpf  replicate 2", "kl 14744 e kidney", null, "strain:AB|age:3months|sex:male|tissue:kidney|genotype:aKlotho|Replicate:Bilogical replicate 2|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Klotho  kidney  3 mpf  replicate 2", "kl 14744 e kidney", "kl 14744 e kidney", "Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills  heart and kidney were collected from 32 individual fish at two time points  namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point   4 males  4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C  and samples were stored at  80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing  mercaptoethanol and stored at  80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. 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In contrast  its overexpression increases lifespan. Given the severe mouse phenotype  we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish  with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body  most dramatically in the outflow tract of the heart  the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. 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RNALater was removed post an overnight incubation at 4C  and samples were stored at  80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing  mercaptoethanol and stored at  80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. 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In contrast  its overexpression increases lifespan. Given the severe mouse phenotype  we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish  with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body  most dramatically in the outflow tract of the heart  the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. 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RNALater was removed post an overnight incubation at 4C  and samples were stored at  80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing  mercaptoethanol and stored at  80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. 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In contrast  its overexpression increases lifespan. Given the severe mouse phenotype  we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish  with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body  most dramatically in the outflow tract of the heart  the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. 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RNALater was removed post an overnight incubation at 4C  and samples were stored at  80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing  mercaptoethanol and stored at  80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. 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In contrast  its overexpression increases lifespan. Given the severe mouse phenotype  we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish  with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body  most dramatically in the outflow tract of the heart  the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.", null, null, null, "\u03b1Klotho  kidney  3 mpf  replicate 5", "kl 14744 d kidney", null, "strain:AB|age:3months|sex:female|tissue:kidney|genotype:aKlotho|Replicate:Bilogical replicate 5|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Klotho  kidney  3 mpf  replicate 5", "kl 14744 d kidney", "kl 14744 d kidney", "Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills  heart and kidney were collected from 32 individual fish at two time points  namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point   4 males  4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C  and samples were stored at  80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing  mercaptoethanol and stored at  80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP216556", null, null, "kl-14744-d-kidney_merged_S94-1.fastq.gz kl-14744-d-kidney_merged_S94-2.fastq.gz", "fastq fastq", 3235564566.0, 21704627.0, "kl 14744 d kidney merged S94 1.fastq.gz", "0:74.55 1:74.53", "A:808568448;C:783575935;G:776803253;T:830140556;N:36476374", 74, 74, null, null, 808568448, 783575935, 776803253, 830140556, 36476374, "SRX6603887", "SRS5168935", "SRA927891", "Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics", "Novartis Institutes for Biomedical Research", 2, 0.95065, 0.95457, 0.07932, 0.0782, 0.69844, 0.69852, 0.52161, 0.52384, 75, 75, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "Germany", "2019-07-27", "Adult", "Adult", "Kidney", "Renal System"], [53299, "SRR9849200", "SRX6603886", "SRS5168936", "SRP216556", "PRJNA556842", "aKlotho regulates age associated vascular calcification and lifespan in zebrafish", "PRJNA556842", "Transcriptome Analysis", "The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast  its overexpression increases lifespan. Given the severe mouse phenotype  we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish  with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body  most dramatically in the outflow tract of the heart  the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.", null, null, null, "\u03b1Klotho  kidney  5 mpf  replicate 7", "kl 14563 b kidney", null, "strain:AB|age:5months|sex:female|tissue:kidney|genotype:aKlotho|Replicate:Bilogical replicate 7|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Klotho  kidney  5 mpf  replicate 7", "kl 14563 b kidney", "kl 14563 b kidney", "Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills  heart and kidney were collected from 32 individual fish at two time points  namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point   4 males  4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C  and samples were stored at  80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing  mercaptoethanol and stored at  80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP216556", null, null, "kl-14563-b-kidney_merged_S23-1.fastq.gz kl-14563-b-kidney_merged_S23-2.fastq.gz", "fastq fastq", 3841497499.0, 25646923.0, "kl 14563 b kidney merged S23 1.fastq.gz", "0:74.90 1:74.89", "A:999048603;C:899322616;G:891418096;T:1023530696;N:28177488", 74, 74, null, null, 999048603, 899322616, 891418096, 1023530696, 28177488, "SRX6603886", "SRS5168936", "SRA927891", "Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics", "Novartis Institutes for Biomedical Research", 2, 0.94562, 0.94897, 0.098, 0.09621, 0.71977, 0.7209, 0.54321, 0.53814, 76, 75, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "Germany", "2019-07-27", "Adult", "Adult", "Kidney", "Renal System"], [53300, "SRR9849206", "SRX6603885", "SRS5168934", "SRP216556", "PRJNA556842", "aKlotho regulates age associated vascular calcification and lifespan in zebrafish", "PRJNA556842", "Transcriptome Analysis", "The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast  its overexpression increases lifespan. Given the severe mouse phenotype  we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish  with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body  most dramatically in the outflow tract of the heart  the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.", null, null, null, "\u03b1Klotho  kidney  5 mpf  replicate 8", "kl 14563 c kidney", null, "strain:AB|age:5months|sex:female|tissue:kidney|genotype:aKlotho|Replicate:Bilogical replicate 8|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Klotho  kidney  5 mpf  replicate 8", "kl 14563 c kidney", "kl 14563 c kidney", "Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills  heart and kidney were collected from 32 individual fish at two time points  namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point   4 males  4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C  and samples were stored at  80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing  mercaptoethanol and stored at  80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP216556", null, null, "kl-14563-c-kidney_merged_S35-1.fastq.gz kl-14563-c-kidney_merged_S35-2.fastq.gz", "fastq fastq", 4215569581.0, 28089663.0, "kl 14563 c kidney merged S35 1.fastq.gz", "0:75.05 1:75.03", "A:1122288966;C:970063144;G:955510223;T:1146500593;N:21206655", 75, 75, null, null, 1122288966, 970063144, 955510223, 1146500593, 21206655, "SRX6603885", "SRS5168934", "SRA927891", "Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics", "Novartis Institutes for Biomedical Research", 2, 0.94171, 0.94373, 0.11361, 0.11133, 0.70136, 0.7025, 0.49345, 0.51472, 76, 76, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "Germany", "2019-07-27", "Adult", "Adult", "Kidney", "Renal System"], [53301, "SRR9849201", "SRX6603884", "SRS5168933", "SRP216556", "PRJNA556842", "aKlotho regulates age associated vascular calcification and lifespan in zebrafish", "PRJNA556842", "Transcriptome Analysis", "The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast  its overexpression increases lifespan. Given the severe mouse phenotype  we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish  with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body  most dramatically in the outflow tract of the heart  the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.", null, null, null, "\u03b1Klotho  kidney  5 mpf  replicate 5", "kl 14562 b kidney", null, "strain:AB|age:5months|sex:female|tissue:kidney|genotype:aKlotho|Replicate:Bilogical replicate 5|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Klotho  kidney  5 mpf  replicate 5", "kl 14562 b kidney", "kl 14562 b kidney", "Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills  heart and kidney were collected from 32 individual fish at two time points  namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point   4 males  4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C  and samples were stored at  80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing  mercaptoethanol and stored at  80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP216556", null, null, "kl-14562-b-kidney_merged_S18-1.fastq.gz kl-14562-b-kidney_merged_S18-2.fastq.gz", "fastq fastq", 3972193490.0, 27155081.0, "kl 14562 b kidney merged S18 1.fastq.gz", "0:73.14 1:73.14", "A:987358107;C:933387787;G:924448548;T:1015576411;N:111422637", 73, 73, null, null, 987358107, 933387787, 924448548, 1015576411, 111422637, "SRX6603884", "SRS5168933", "SRA927891", "Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics", "Novartis Institutes for Biomedical Research", 2, 0.94949, 0.95176, 0.08272, 0.08121, 0.7166, 0.71705, 0.53783, 0.53989, 76, 76, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "Germany", "2019-07-27", "Adult", "Adult", "Kidney", "Renal System"], [53302, "SRR9849205", "SRX6603883", "SRS5168932", "SRP216556", "PRJNA556842", "aKlotho regulates age associated vascular calcification and lifespan in zebrafish", "PRJNA556842", "Transcriptome Analysis", "The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast  its overexpression increases lifespan. Given the severe mouse phenotype  we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish  with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body  most dramatically in the outflow tract of the heart  the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.", null, null, null, "\u03b1Klotho  kidney  5 mpf  replicate 6", "kl 14562 d kidney", null, "strain:AB|age:5months|sex:female|tissue:kidney|genotype:aKlotho|Replicate:Bilogical replicate 6|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Klotho  kidney  5 mpf  replicate 6", "kl 14562 d kidney", "kl 14562 d kidney", "Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills  heart and kidney were collected from 32 individual fish at two time points  namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point   4 males  4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C  and samples were stored at  80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing  mercaptoethanol and stored at  80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP216556", null, null, "kl-14562-d-kidney_merged_S42-1.fastq.gz kl-14562-d-kidney_merged_S42-2.fastq.gz", "fastq fastq", 3526368194.0, 23516128.0, "kl 14562 d kidney merged S42 1.fastq.gz", "0:75.00 1:74.95", "A:869319867;C:871562247;G:869066601;T:893717214;N:22702265", 75, 74, null, null, 869319867, 871562247, 869066601, 893717214, 22702265, "SRX6603883", "SRS5168932", "SRA927891", "Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics", "Novartis Institutes for Biomedical Research", 2, 0.96175, 0.96369, 0.06361, 0.06218, 0.70905, 0.71157, 0.51899, 0.52384, 76, 76, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "Germany", "2019-07-27", "Adult", "Adult", "Kidney", "Renal System"], [53303, "SRR9849202", "SRX6603882", "SRS5168931", "SRP216556", "PRJNA556842", "aKlotho regulates age associated vascular calcification and lifespan in zebrafish", "PRJNA556842", "Transcriptome Analysis", "The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast  its overexpression increases lifespan. Given the severe mouse phenotype  we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish  with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body  most dramatically in the outflow tract of the heart  the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.", null, null, null, "\u03b1Klotho  kidney  5 mpf  replicate 3", "kl 14563 a kidney", null, "strain:AB|age:5months|sex:male|tissue:kidney|genotype:aKlotho|Replicate:Bilogical replicate 3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Klotho  kidney  5 mpf  replicate 3", "kl 14563 a kidney", "kl 14563 a kidney", "Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills  heart and kidney were collected from 32 individual fish at two time points  namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point   4 males  4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C  and samples were stored at  80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing  mercaptoethanol and stored at  80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP216556", null, null, "kl-14563-a-kidney_merged_S11-1.fastq.gz kl-14563-a-kidney_merged_S11-2.fastq.gz", "fastq fastq", 4326402378.0, 29503938.0, "kl 14563 a kidney merged S11 1.fastq.gz", "0:73.31 1:73.33", "A:1085969692;C:1017480039;G:1010619557;T:1105911425;N:106421665", 73, 73, null, null, 1085969692, 1017480039, 1010619557, 1105911425, 106421665, "SRX6603882", "SRS5168931", "SRA927891", "Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics", "Novartis Institutes for Biomedical Research", 2, 0.94169, 0.94864, 0.08735, 0.0856, 0.74018, 0.74063, 0.532, 0.54233, 35, 35, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "Germany", "2019-07-27", "Adult", "Adult", "Kidney", "Renal System"], [53304, "SRR9849203", "SRX6603881", "SRS5168930", "SRP216556", "PRJNA556842", "aKlotho regulates age associated vascular calcification and lifespan in zebrafish", "PRJNA556842", "Transcriptome Analysis", "The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast  its overexpression increases lifespan. Given the severe mouse phenotype  we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish  with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body  most dramatically in the outflow tract of the heart  the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.", null, null, null, "\u03b1Klotho  kidney  5 mpf  replicate 1", "kl 14562 a kidney", null, "strain:AB|age:5months|sex:male|tissue:kidney|genotype:aKlotho|Replicate:Bilogical replicate 1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Klotho  kidney  5 mpf  replicate 1", "kl 14562 a kidney", "kl 14562 a kidney", "Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills  heart and kidney were collected from 32 individual fish at two time points  namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point   4 males  4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C  and samples were stored at  80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing  mercaptoethanol and stored at  80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP216556", null, null, "kl-14562-a-kidney_merged_S6-1.fastq.gz kl-14562-a-kidney_merged_S6-2.fastq.gz", "fastq fastq", 4980501349.0, 34004455.0, "kl 14562 a kidney merged S6 1.fastq.gz", "0:73.23 1:73.24", "A:1251556852;C:1167350759;G:1159943717;T:1277350171;N:124299850", 73, 73, null, null, 1251556852, 1167350759, 1159943717, 1277350171, 124299850, "SRX6603881", "SRS5168930", "SRA927891", "Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics", "Novartis Institutes for Biomedical Research", 2, 0.94619, 0.94971, 0.08823, 0.08683, 0.72807, 0.72796, 0.51972, 0.52145, 35, 35, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "Germany", "2019-07-27", "Adult", "Adult", "Kidney", "Renal System"], [53305, "SRR9849204", "SRX6603880", "SRS5168929", "SRP216556", "PRJNA556842", "aKlotho regulates age associated vascular calcification and lifespan in zebrafish", "PRJNA556842", "Transcriptome Analysis", "The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast  its overexpression increases lifespan. Given the severe mouse phenotype  we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish  with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body  most dramatically in the outflow tract of the heart  the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.", null, null, null, "\u03b1Klotho  kidney  5 mpf  replicate 4", "kl 14563 d kidney", null, "strain:AB|age:5months|sex:male|tissue:kidney|genotype:aKlotho|Replicate:Bilogical replicate 4|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Klotho  kidney  5 mpf  replicate 4", "kl 14563 d kidney", "kl 14563 d kidney", "Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills  heart and kidney were collected from 32 individual fish at two time points  namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point   4 males  4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C  and samples were stored at  80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing  mercaptoethanol and stored at  80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP216556", null, null, "kl-14563-d-kidney_merged_S47-1.fastq.gz kl-14563-d-kidney_merged_S47-2.fastq.gz", "fastq fastq", 6869441307.0, 56338972.0, "kl 14563 d kidney merged S47 1.fastq.gz", "0:60.76 1:61.17", "A:1348721440;C:1368998259;G:1366726420;T:1380954564;N:1404040624", 60, 61, null, null, 1348721440, 1368998259, 1366726420, 1380954564, 1404040624, "SRX6603880", "SRS5168929", "SRA927891", "Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics", "Novartis Institutes for Biomedical Research", 2, 0.96257, 0.96453, 0.06751, 0.06671, 0.75548, 0.75607, 0.51978, 0.52832, 74, 76, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "Germany", "2019-07-27", "Adult", "Adult", "Kidney", "Renal System"], [53306, "SRR9849207", "SRX6603879", "SRS5168928", "SRP216556", "PRJNA556842", "aKlotho regulates age associated vascular calcification and lifespan in zebrafish", "PRJNA556842", "Transcriptome Analysis", "The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast  its overexpression increases lifespan. Given the severe mouse phenotype  we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish  with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body  most dramatically in the outflow tract of the heart  the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.", null, null, null, "\u03b1Klotho  kidney  5 mpf  replicate 2", "kl 14562 c kidney", null, "strain:AB|age:5months|sex:male|tissue:kidney|genotype:aKlotho|Replicate:Bilogical replicate 2|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Klotho  kidney  5 mpf  replicate 2", "kl 14562 c kidney", "kl 14562 c kidney", "Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills  heart and kidney were collected from 32 individual fish at two time points  namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point   4 males  4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C  and samples were stored at  80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing  mercaptoethanol and stored at  80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP216556", null, null, "kl-14562-c-kidney_merged_S30-1.fastq.gz kl-14562-c-kidney_merged_S30-2.fastq.gz", "fastq fastq", 3320801196.0, 22339756.0, "kl 14562 c kidney merged S30 1.fastq.gz", "0:74.33 1:74.32", "A:856878404;C:774178244;G:769653230;T:875894981;N:44196337", 74, 74, null, null, 856878404, 774178244, 769653230, 875894981, 44196337, "SRX6603879", "SRS5168928", "SRA927891", "Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics", "Novartis Institutes for Biomedical Research", 2, 0.95411, 0.95756, 0.08333, 0.08245, 0.7162, 0.71638, 0.56576, 0.55653, 74, 76, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "Germany", "2019-07-27", "Adult", "Adult", "Kidney", "Renal System"], [53307, "SRR9849213", "SRX6603878", "SRS5168927", "SRP216556", "PRJNA556842", "aKlotho regulates age associated vascular calcification and lifespan in zebrafish", "PRJNA556842", "Transcriptome Analysis", "The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast  its overexpression increases lifespan. Given the severe mouse phenotype  we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish  with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body  most dramatically in the outflow tract of the heart  the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.", null, null, null, "\u03b1Klotho  kidney  3 mpf  replicate 6", "kl 14744 f kidney", null, "strain:AB|age:3months|sex:female|tissue:kidney|genotype:aKlotho|Replicate:Bilogical replicate 6|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Klotho  kidney  3 mpf  replicate 6", "kl 14744 f kidney", "kl 14744 f kidney", "Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills  heart and kidney were collected from 32 individual fish at two time points  namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point   4 males  4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C  and samples were stored at  80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing  mercaptoethanol and stored at  80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP216556", null, null, "kl-14744-f-kidney_merged_S71-1.fastq.gz kl-14744-f-kidney_merged_S71-2.fastq.gz", "fastq fastq", 2801226765.0, 18731091.0, "kl 14744 f kidney merged S71 1.fastq.gz", "0:74.79 1:74.76", "A:720950274;C:664580809;G:653567624;T:738645101;N:23482957", 74, 74, null, null, 720950274, 664580809, 653567624, 738645101, 23482957, "SRX6603878", "SRS5168927", "SRA927891", "Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics", "Novartis Institutes for Biomedical Research", 2, 0.94692, 0.95016, 0.10024, 0.09917, 0.69781, 0.69745, 0.53063, 0.53615, 76, 76, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "Germany", "2019-07-27", "Adult", "Adult", "Kidney", "Renal System"], [53308, "SRR9849208", "SRX6603877", "SRS5168926", "SRP216556", "PRJNA556842", "aKlotho regulates age associated vascular calcification and lifespan in zebrafish", "PRJNA556842", "Transcriptome Analysis", "The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast  its overexpression increases lifespan. Given the severe mouse phenotype  we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish  with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body  most dramatically in the outflow tract of the heart  the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.", null, null, null, "\u03b1Klotho  kidney  3 mpf  replicate 7", "kl 14744 h kidney", null, "strain:AB|age:3months|sex:female|tissue:kidney|genotype:aKlotho|Replicate:Bilogical replicate 7|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Klotho  kidney  3 mpf  replicate 7", "kl 14744 h kidney", "kl 14744 h kidney", "Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills  heart and kidney were collected from 32 individual fish at two time points  namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point   4 males  4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C  and samples were stored at  80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing  mercaptoethanol and stored at  80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP216556", null, null, "kl-14744-h-kidney_merged_S95-1.fastq.gz kl-14744-h-kidney_merged_S95-2.fastq.gz", "fastq fastq", 3606112938.0, 24298531.0, "kl 14744 h kidney merged S95 1.fastq.gz", "0:74.21 1:74.20", "A:907594636;C:863534560;G:852256795;T:929423242;N:53303705", 74, 74, null, null, 907594636, 863534560, 852256795, 929423242, 53303705, "SRX6603877", "SRS5168926", "SRA927891", "Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics", "Novartis Institutes for Biomedical Research", 2, 0.95343, 0.95612, 0.07895, 0.07817, 0.70944, 0.71023, 0.54468, 0.54382, 76, 76, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "Germany", "2019-07-27", "Adult", "Adult", "Kidney", "Renal System"], [53309, "SRR9849212", "SRX6603876", "SRS5168925", "SRP216556", "PRJNA556842", "aKlotho regulates age associated vascular calcification and lifespan in zebrafish", "PRJNA556842", "Transcriptome Analysis", "The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast  its overexpression increases lifespan. Given the severe mouse phenotype  we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish  with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body  most dramatically in the outflow tract of the heart  the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.", null, null, null, "wildtype  kidney  5 mpf  replicate 2", "wt 14560 c kidney", null, "strain:AB|age:5months|sex:male|tissue:kidney|genotype:wildtype|Replicate:Bilogical replicate 2|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "wildtype  kidney  5 mpf  replicate 2", "wt 14560 c kidney", "wt 14560 c kidney", "Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills  heart and kidney were collected from 32 individual fish at two time points  namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point   4 males  4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C  and samples were stored at  80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing  mercaptoethanol and stored at  80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP216556", null, null, "wt-14560-c-kidney_merged_S29-1.fastq.gz wt-14560-c-kidney_merged_S29-2.fastq.gz", "fastq fastq", 3091657773.0, 21774524.0, "wt 14560 c kidney merged S29 1.fastq.gz", "0:70.96 1:71.03", "A:763113194;C:693515052;G:687380502;T:778507279;N:169141746", 70, 71, null, null, 763113194, 693515052, 687380502, 778507279, 169141746, "SRX6603876", "SRS5168925", "SRA927891", "Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics", "Novartis Institutes for Biomedical Research", 2, 0.95062, 0.95349, 0.09247, 0.09119, 0.72754, 0.72863, 0.53643, 0.54181, 76, 76, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "Germany", "2019-07-27", "Adult", "Adult", "Kidney", "Renal System"], [53310, "SRR9849209", "SRX6603875", "SRS5168924", "SRP216556", "PRJNA556842", "aKlotho regulates age associated vascular calcification and lifespan in zebrafish", "PRJNA556842", "Transcriptome Analysis", "The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast  its overexpression increases lifespan. Given the severe mouse phenotype  we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish  with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body  most dramatically in the outflow tract of the heart  the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.", null, null, null, "wildtype  kidney  5 mpf  replicate 3", "wt 14560 e kidney", null, "strain:AB|age:5months|sex:male|tissue:kidney|genotype:wildtype|Replicate:Bilogical replicate 3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "wildtype  kidney  5 mpf  replicate 3", "wt 14560 e kidney", "wt 14560 e kidney", "Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills  heart and kidney were collected from 32 individual fish at two time points  namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point   4 males  4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C  and samples were stored at  80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing  mercaptoethanol and stored at  80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP216556", null, null, "wt-14560-e-kidney_merged_S53-1.fastq.gz wt-14560-e-kidney_merged_S53-2.fastq.gz", "fastq fastq", 3242753931.0, 21639832.0, "wt 14560 e kidney merged S53 1.fastq.gz", "0:74.93 1:74.92", "A:843085674;C:760074682;G:756945733;T:860752379;N:21895463", 74, 74, null, null, 843085674, 760074682, 756945733, 860752379, 21895463, "SRX6603875", "SRS5168924", "SRA927891", "Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics", "Novartis Institutes for Biomedical Research", 2, 0.95058, 0.95272, 0.0982, 0.09719, 0.71707, 0.71825, 0.5497, 0.53642, 75, 76, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "Germany", "2019-07-27", "Adult", "Adult", "Kidney", "Renal System"], [53311, "SRR9849210", "SRX6603874", "SRS5168923", "SRP216556", "PRJNA556842", "aKlotho regulates age associated vascular calcification and lifespan in zebrafish", "PRJNA556842", "Transcriptome Analysis", "The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast  its overexpression increases lifespan. Given the severe mouse phenotype  we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish  with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body  most dramatically in the outflow tract of the heart  the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.", null, null, null, "wildtype  kidney  3 mpf  replicate 7", "wt 14746 g kidney", null, "strain:AB|age:3months|sex:female|tissue:kidney|genotype:wildtype|Replicate:Bilogical replicate 7|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "wildtype  kidney  3 mpf  replicate 7", "wt 14746 g kidney", "wt 14746 g kidney", "Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills  heart and kidney were collected from 32 individual fish at two time points  namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point   4 males  4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C  and samples were stored at  80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing  mercaptoethanol and stored at  80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP216556", null, null, "wt-14746-g-kidney_merged_S22-1.fastq.gz wt-14746-g-kidney_merged_S22-2.fastq.gz", "fastq fastq", 3928427553.0, 26318173.0, "wt 14746 g kidney merged S22 1.fastq.gz", "0:74.63 1:74.64", "A:1019611627;C:916883865;G:906743219;T:1044351913;N:40836929", 74, 74, null, null, 1019611627, 916883865, 906743219, 1044351913, 40836929, "SRX6603874", "SRS5168923", "SRA927891", "Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics", "Novartis Institutes for Biomedical Research", 2, 0.94281, 0.94563, 0.09937, 0.0975, 0.71478, 0.71585, 0.54524, 0.5444, 76, 74, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "Germany", "2019-07-27", "Adult", "Adult", "Kidney", "Renal System"], [53312, "SRR9849211", "SRX6603873", "SRS5168922", "SRP216556", "PRJNA556842", "aKlotho regulates age associated vascular calcification and lifespan in zebrafish", "PRJNA556842", "Transcriptome Analysis", "The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast  its overexpression increases lifespan. Given the severe mouse phenotype  we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish  with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body  most dramatically in the outflow tract of the heart  the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.", null, null, null, "wildtype  kidney  3 mpf  replicate 6", "wt 14746 d kidney", null, "strain:AB|age:3months|sex:female|tissue:kidney|genotype:wildtype|Replicate:Bilogical replicate 6|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "wildtype  kidney  3 mpf  replicate 6", "wt 14746 d kidney", "wt 14746 d kidney", "Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills  heart and kidney were collected from 32 individual fish at two time points  namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point   4 males  4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C  and samples were stored at  80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing  mercaptoethanol and stored at  80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP216556", null, null, "wt-14746-d-kidney_merged_S81-2.fastq.gz wt-14746-d-kidney_merged_S81-1.fastq.gz", "fastq fastq", 3235839633.0, 21863802.0, "wt 14746 d kidney merged S81 1.fastq.gz", "0:73.99 1:74.01", "A:819433052;C:762877585;G:759574301;T:837534821;N:56419874", 73, 74, null, null, 819433052, 762877585, 759574301, 837534821, 56419874, "SRX6603873", "SRS5168922", "SRA927891", "Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics", "Novartis Institutes for Biomedical Research", 2, 0.94986, 0.95295, 0.09789, 0.09661, 0.72109, 0.72344, 0.5002, 0.53439, 75, 75, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "Germany", "2019-07-27", "Adult", "Adult", "Kidney", "Renal System"], [53313, "SRR9849214", "SRX6603872", "SRS5168921", "SRP216556", "PRJNA556842", "aKlotho regulates age associated vascular calcification and lifespan in zebrafish", "PRJNA556842", "Transcriptome Analysis", "The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast  its overexpression increases lifespan. Given the severe mouse phenotype  we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish  with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body  most dramatically in the outflow tract of the heart  the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.", null, null, null, "wildtype  kidney  3 mpf  replicate 8", "wt 14746 i kidney", null, "strain:AB|age:3months|sex:female|tissue:kidney|genotype:wildtype|Replicate:Bilogical replicate 8|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "wildtype  kidney  3 mpf  replicate 8", "wt 14746 i kidney", "wt 14746 i kidney", "Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills  heart and kidney were collected from 32 individual fish at two time points  namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point   4 males  4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C  and samples were stored at  80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing  mercaptoethanol and stored at  80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP216556", null, null, "wt-14746-i-kidney_merged_S46-1.fastq.gz wt-14746-i-kidney_merged_S46-2.fastq.gz", "fastq fastq", 3377453037.0, 23023990.0, "wt 14746 i kidney merged S46 1.fastq.gz", "0:73.35 1:73.34", "A:836450075;C:803489724;G:795497926;T:858148995;N:83866317", 73, 73, null, null, 836450075, 803489724, 795497926, 858148995, 83866317, "SRX6603872", "SRS5168921", "SRA927891", "Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics", "Novartis Institutes for Biomedical Research", 2, 0.95192, 0.95558, 0.0795, 0.07926, 0.70589, 0.70619, 0.52743, 0.52432, 76, 76, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "Germany", "2019-07-27", "Adult", "Adult", "Kidney", "Renal System"], [53314, "SRR9849220", "SRX6603871", "SRS5168920", "SRP216556", "PRJNA556842", "aKlotho regulates age associated vascular calcification and lifespan in zebrafish", "PRJNA556842", "Transcriptome Analysis", "The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast  its overexpression increases lifespan. Given the severe mouse phenotype  we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish  with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body  most dramatically in the outflow tract of the heart  the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.", null, null, null, "wildtype  kidney  5 mpf  replicate 1", "wt 14560 a kidney", null, "strain:AB|age:5months|sex:male|tissue:kidney|genotype:wildtype|Replicate:Bilogical replicate 1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "wildtype  kidney  5 mpf  replicate 1", "wt 14560 a kidney", "wt 14560 a kidney", "Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills  heart and kidney were collected from 32 individual fish at two time points  namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point   4 males  4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C  and samples were stored at  80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing  mercaptoethanol and stored at  80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP216556", null, null, "wt-14560-a-kidney_merged_S5-1.fastq.gz wt-14560-a-kidney_merged_S5-2.fastq.gz", "fastq fastq", 3803097028.0, 29032173.0, "wt 14560 a kidney merged S5 1.fastq.gz", "0:65.34 1:65.66", "A:880043540;C:764446357;G:759630921;T:898342437;N:500633773", 65, 65, null, null, 880043540, 764446357, 759630921, 898342437, 500633773, "SRX6603871", "SRS5168920", "SRA927891", "Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics", "Novartis Institutes for Biomedical Research", 2, 0.94594, 0.94896, 0.10801, 0.10661, 0.74292, 0.74379, 0.56139, 0.56212, 76, 75, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "Germany", "2019-07-27", "Adult", "Adult", "Kidney", "Renal System"], [53315, "SRR9849215", "SRX6603870", "SRS5168919", "SRP216556", "PRJNA556842", "aKlotho regulates age associated vascular calcification and lifespan in zebrafish", "PRJNA556842", "Transcriptome Analysis", "The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast  its overexpression increases lifespan. Given the severe mouse phenotype  we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish  with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body  most dramatically in the outflow tract of the heart  the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.", null, null, null, "wildtype  kidney  3 mpf  replicate 2", "wt 14746 e kidney", null, "strain:AB|age:3months|sex:male|tissue:kidney|genotype:wildtype|Replicate:Bilogical replicate 2|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "wildtype  kidney  3 mpf  replicate 2", "wt 14746 e kidney", "wt 14746 e kidney", "Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills  heart and kidney were collected from 32 individual fish at two time points  namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point   4 males  4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C  and samples were stored at  80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing  mercaptoethanol and stored at  80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP216556", null, null, "wt-14746-e-kidney_merged_S93-1.fastq.gz wt-14746-e-kidney_merged_S93-2.fastq.gz", "fastq fastq", 3389715259.0, 23030176.0, "wt 14746 e kidney merged S93 1.fastq.gz", "0:73.58 1:73.60", "A:845566798;C:806510240;G:801049741;T:861758601;N:74829879", 73, 73, null, null, 845566798, 806510240, 801049741, 861758601, 74829879, "SRX6603870", "SRS5168919", "SRA927891", "Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics", "Novartis Institutes for Biomedical Research", 2, 0.96201, 0.96536, 0.07181, 0.0708, 0.73594, 0.73628, 0.5625, 0.57023, 76, 76, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "Germany", "2019-07-27", "Adult", "Adult", "Kidney", "Renal System"], [53316, "SRR9849219", "SRX6603869", "SRS5168918", "SRP216556", "PRJNA556842", "aKlotho regulates age associated vascular calcification and lifespan in zebrafish", "PRJNA556842", "Transcriptome Analysis", "The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast  its overexpression increases lifespan. Given the severe mouse phenotype  we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish  with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body  most dramatically in the outflow tract of the heart  the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.", null, null, null, "wildtype  kidney  3 mpf  replicate 3", "wt 14746 f kidney", null, "strain:AB|age:3months|sex:male|tissue:kidney|genotype:wildtype|Replicate:Bilogical replicate 3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "wildtype  kidney  3 mpf  replicate 3", "wt 14746 f kidney", "wt 14746 f kidney", "Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills  heart and kidney were collected from 32 individual fish at two time points  namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point   4 males  4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C  and samples were stored at  80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing  mercaptoethanol and stored at  80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP216556", null, null, "wt-14746-f-kidney_merged_S10-1.fastq.gz wt-14746-f-kidney_merged_S10-2.fastq.gz", "fastq fastq", 5697834151.0, 38061527.0, "wt 14746 f kidney merged S10 1.fastq.gz", "0:74.86 1:74.84", "A:1488491305;C:1328009157;G:1311260167;T:1524663985;N:45409537", 74, 74, null, null, 1488491305, 1328009157, 1311260167, 1524663985, 45409537, "SRX6603869", "SRS5168918", "SRA927891", "Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics", "Novartis Institutes for Biomedical Research", 2, 0.94873, 0.9525, 0.0943, 0.09336, 0.71709, 0.71741, 0.53907, 0.55371, 76, 75, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "Germany", "2019-07-27", "Adult", "Adult", "Kidney", "Renal System"], [53317, "SRR9849216", "SRX6603868", "SRS5168917", "SRP216556", "PRJNA556842", "aKlotho regulates age associated vascular calcification and lifespan in zebrafish", "PRJNA556842", "Transcriptome Analysis", "The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast  its overexpression increases lifespan. Given the severe mouse phenotype  we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish  with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body  most dramatically in the outflow tract of the heart  the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.", null, null, null, "wildtype  kidney  3 mpf  replicate 4", "wt 14746 h kidney", null, "strain:AB|age:3months|sex:male|tissue:kidney|genotype:wildtype|Replicate:Bilogical replicate 4|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "wildtype  kidney  3 mpf  replicate 4", "wt 14746 h kidney", "wt 14746 h kidney", "Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills  heart and kidney were collected from 32 individual fish at two time points  namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point   4 males  4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C  and samples were stored at  80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing  mercaptoethanol and stored at  80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP216556", null, null, "wt-14746-h-kidney_merged_S34-1.fastq.gz wt-14746-h-kidney_merged_S34-2.fastq.gz", "fastq fastq", 4802949250.0, 32003702.0, "wt 14746 h kidney merged S34 1.fastq.gz", "0:75.05 1:75.02", "A:1247408978;C:1132423247;G:1119030906;T:1277389500;N:26696619", 75, 75, null, null, 1247408978, 1132423247, 1119030906, 1277389500, 26696619, "SRX6603868", "SRS5168917", "SRA927891", "Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics", "Novartis Institutes for Biomedical Research", 2, 0.94868, 0.95207, 0.1008, 0.1002, 0.70654, 0.70583, 0.543, 0.53646, 76, 76, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "Germany", "2019-07-27", "Adult", "Adult", "Kidney", "Renal System"], [53318, "SRR9849217", "SRX6603867", "SRS5168916", "SRP216556", "PRJNA556842", "aKlotho regulates age associated vascular calcification and lifespan in zebrafish", "PRJNA556842", "Transcriptome Analysis", "The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast  its overexpression increases lifespan. Given the severe mouse phenotype  we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish  with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body  most dramatically in the outflow tract of the heart  the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.", null, null, null, "wildtype  kidney  3 mpf  replicate 1", "wt 14746 c kidney", null, "strain:AB|age:3months|sex:male|tissue:kidney|genotype:wildtype|Replicate:Bilogical replicate 1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "wildtype  kidney  3 mpf  replicate 1", "wt 14746 c kidney", "wt 14746 c kidney", "Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills  heart and kidney were collected from 32 individual fish at two time points  namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point   4 males  4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C  and samples were stored at  80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing  mercaptoethanol and stored at  80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP216556", null, null, "wt-14746-c-kidney_merged_S69-1.fastq.gz wt-14746-c-kidney_merged_S69-2.fastq.gz", "fastq fastq", 2926155789.0, 19616550.0, "wt 14746 c kidney merged S69 1.fastq.gz", "0:74.59 1:74.58", "A:738508985;C:703442172;G:697795154;T:755346949;N:31062529", 74, 74, null, null, 738508985, 703442172, 697795154, 755346949, 31062529, "SRX6603867", "SRS5168916", "SRA927891", "Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics", "Novartis Institutes for Biomedical Research", 2, 0.95391, 0.95737, 0.08073, 0.08025, 0.72273, 0.72265, 0.50256, 0.53781, 76, 75, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "Germany", "2019-07-27", "Adult", "Adult", "Kidney", "Renal System"], [53319, "SRR9849218", "SRX6603866", "SRS5168915", "SRP216556", "PRJNA556842", "aKlotho regulates age associated vascular calcification and lifespan in zebrafish", "PRJNA556842", "Transcriptome Analysis", "The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast  its overexpression increases lifespan. Given the severe mouse phenotype  we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish  with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body  most dramatically in the outflow tract of the heart  the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.", null, null, null, "wildtype  kidney  3 mpf  replicate 5", "wt 14746 b kidney", null, "strain:AB|age:3months|sex:female|tissue:kidney|genotype:wildtype|Replicate:Bilogical replicate 5|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "wildtype  kidney  3 mpf  replicate 5", "wt 14746 b kidney", "wt 14746 b kidney", "Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills  heart and kidney were collected from 32 individual fish at two time points  namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point   4 males  4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C  and samples were stored at  80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing  mercaptoethanol and stored at  80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP216556", null, null, "wt-14746-b-kidney_merged_S57-1.fastq.gz wt-14746-b-kidney_merged_S57-2.fastq.gz", "fastq fastq", 3580693879.0, 24148496.0, "wt 14746 b kidney merged S57 1.fastq.gz", "0:74.13 1:74.15", "A:906877863;C:847589932;G:842258575;T:927440495;N:56527014", 74, 74, null, null, 906877863, 847589932, 842258575, 927440495, 56527014, "SRX6603866", "SRS5168915", "SRA927891", "Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics", "Novartis Institutes for Biomedical Research", 2, 0.94558, 0.95003, 0.09575, 0.09468, 0.72099, 0.72192, 0.52455, 0.51579, 75, 76, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "Germany", "2019-07-27", "Adult", "Adult", "Kidney", "Renal System"], [53377, "SRR9849279", "SRX6603808", "SRS5168857", "SRP216556", "PRJNA556842", "aKlotho regulates age associated vascular calcification and lifespan in zebrafish", "PRJNA556842", "Transcriptome Analysis", "The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast  its overexpression increases lifespan. Given the severe mouse phenotype  we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish  with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body  most dramatically in the outflow tract of the heart  the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.", null, null, null, "wildtype  kidney  5 mpf  replicate 7", "wt 14560 f kidney", null, "strain:AB|age:5months|sex:female|tissue:kidney|genotype:wildtype|Replicate:Bilogical replicate 7|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "wildtype  kidney  5 mpf  replicate 7", "wt 14560 f kidney", "wt 14560 f kidney", "Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills  heart and kidney were collected from 32 individual fish at two time points  namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point   4 males  4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C  and samples were stored at  80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing  mercaptoethanol and stored at  80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP216556", null, null, "wt-14560-f-kidney_merged_S65-1.fastq.gz wt-14560-f-kidney_merged_S65-2.fastq.gz", "fastq fastq", 3381175351.0, 22760562.0, "wt 14560 f kidney merged S65 1.fastq.gz", "0:74.29 1:74.26", "A:834174555;C:827862556;G:819581002;T:856481808;N:43075430", 74, 74, null, null, 834174555, 827862556, 819581002, 856481808, 43075430, "SRX6603808", "SRS5168857", "SRA927891", "Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics", "Novartis Institutes for Biomedical Research", 2, 0.96026, 0.96284, 0.07018, 0.06939, 0.69662, 0.69647, 0.48686, 0.48311, 76, 76, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "Germany", "2019-07-27", "Adult", "Adult", "Kidney", "Renal System"], [53378, "SRR9849280", "SRX6603807", "SRS5168856", "SRP216556", "PRJNA556842", "aKlotho regulates age associated vascular calcification and lifespan in zebrafish", "PRJNA556842", "Transcriptome Analysis", "The hormone aKlotho regulates lifespan in mice as knockouts die early of what appears to be accelerated aging due to hyperphosphatemia and soft tissue calcification. In contrast  its overexpression increases lifespan. Given the severe mouse phenotype  we generated zebrafish mutants for aklotho as well as its binding partner fibroblast growth factor 23 fgf23. Both mutations cause shortened lifespan in zebrafish  with abrupt onset of behavioral and degenerative physical changes at around five months of age. There is calcification of vessels throughout the body  most dramatically in the outflow tract of the heart  the bulbus arteriosus BA. This calcification is associated with ectopic activation of osteoclast differentiation pathways. These findings suggest that the gradual loss of aKlotho found in normal aging might give rise to ectopic calcification.", null, null, null, "wildtype  kidney  5 mpf  replicate 6", "wt 14560 d kidney", null, "strain:AB|age:5months|sex:female|tissue:kidney|genotype:wildtype|Replicate:Bilogical replicate 6|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "wildtype  kidney  5 mpf  replicate 6", "wt 14560 d kidney", "wt 14560 d kidney", "Tissue collection and dissection: Adult zebrafish were euthanized by exposure to chilled water 0 4C. Gills  heart and kidney were collected from 32 individual fish at two time points  namely at 3 mpf and 5 mpf for klotho mutants and wildtype sibling controls AB background; 8 fish per genotype per time point   4 males  4 females for a total of 96 samples. Tissues were dissected in cold PBS and immediately stored in RNALater Stabilization Solution ThermoFisher Cat# AM7021. RNALater was removed post an overnight incubation at 4C  and samples were stored at  80C until processing. RNA extraction: All tissues were homogenized using the TissueLyser II Qiagen plus Lysis buffer containing  mercaptoethanol and stored at  80C. RNA extraction was performed using the automated protocol in the QIAcube workstation utilizing the RNeasy Fibrous Tissue Mini kit Qiagen Cat No./ID: 74704. RNA integrity and quality were assessed by Agilent TapeStation using High Sensitivity RNA ScreenTapes. Samples were normalized and 300ng of RNA was used for library prep for each sample. ERCC RNA Spike in mix was added for quality control. mRNA was captured using poly T oligos attached beads for library selection.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP216556", null, null, "wt-14560-d-kidney_merged_S41-1.fastq.gz wt-14560-d-kidney_merged_S41-2.fastq.gz", "fastq fastq", 3260080878.0, 21943002.0, "wt 14560 d kidney merged S41 1.fastq.gz", "0:74.31 1:74.26", "A:781693169;C:817346676;G:813482706;T:801873503;N:45684824", 74, 74, null, null, 781693169, 817346676, 813482706, 801873503, 45684824, "SRX6603807", "SRS5168856", "SRA927891", "Novartis Institutes for Biomedical Research|Chemical Biology and Therapeutics", "Novartis Institutes for Biomedical Research", 2, 0.96382, 0.96558, 0.05582, 0.05496, 0.72245, 0.72342, 0.50059, 0.50428, 76, 76, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "Germany", "2019-07-27", "Adult", "Adult", "Kidney", "Renal System"], [62687, "SRR13319605", "SRX9747096", "SRS7934718", "SRP299583", "PRJNA688352", "Transcriptome Sequencing of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells", "PRJNA688352", "Other", "A transcriptome analysis of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells", null, null, null, "Mut 10d k1", "Mut 10d k1", null, "strain:AB|isolate:missing|breed:missing|cultivar:missing|ecotype:missing|age:15 month|sex:not collected|tissue:kidney and headkidney|biomaterial provider:Southwest University  China|replicate:Biological replicate 1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio: kidney and headkidney from  wild type and congenitally asplenic zebrafish post vaccination", "Mut 10d k1", "Mut 10d k1", "other", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "HiSeq X Ten", null, "SRP299583", null, null, "Mut_10d_k1.raw_1.fastq.gz Mut_10d_k1.raw_2.fastq.gz", "fastq fastq", 6906287100.0, 23020957.0, "Mut 10d k1.raw 1.fastq.gz", "0:150 1:150", "A:1908295029;C:1526919757;G:1627893432;T:1843041112;N:137770", 150, 150, null, null, 1908295029, 1526919757, 1627893432, 1843041112, 137770, "SRX9747096", "SRS7934718", "SRA1178602", "southwest university|college of fisheries", "southwest university", 2, 0.94038, 0.93872, 0.08297, 0.08272, 0.71382, 0.71539, 0.54124, 0.54083, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "other", "unknown", "bulk", "unknown", "unknown", null, "China", "2020-12-28", "Adult", "Adult", "Kidney", "Renal System"], [62688, "SRR13319606", "SRX9747095", "SRS7934719", "SRP299583", "PRJNA688352", "Transcriptome Sequencing of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells", "PRJNA688352", "Other", "A transcriptome analysis of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells", null, null, null, "WT 10d k3", "WT 10d k3", null, "strain:AB|isolate:missing|breed:missing|cultivar:missing|ecotype:missing|age:14 month|sex:not collected|tissue:kidney and headkidney|biomaterial provider:Southwest University  China|replicate:Biological replicate 3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio: kidney and headkidney from  wild type and congenitally asplenic zebrafish post vaccination", "WT 10d k3", "WT 10d k3", "other", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "HiSeq X Ten", null, "SRP299583", null, null, "WT_10d_k3.raw_1.fastq.gz WT_10d_k3.raw_2.fastq.gz", "fastq fastq", 7159590600.0, 23865302.0, "WT 10d k3.raw 1.fastq.gz", "0:150 1:150", "A:1984034638;C:1574065265;G:1674336416;T:1927009990;N:144291", 150, 150, null, null, 1984034638, 1574065265, 1674336416, 1927009990, 144291, "SRX9747095", "SRS7934719", "SRA1178602", "southwest university|college of fisheries", "southwest university", 2, 0.92946, 0.92803, 0.08386, 0.08381, 0.71052, 0.71074, 0.51146, 0.51825, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "other", "unknown", "bulk", "unknown", "unknown", null, "China", "2020-12-28", "Adult", "Adult", "Kidney", "Renal System"], [62689, "SRR13319607", "SRX9747094", "SRS7934714", "SRP299583", "PRJNA688352", "Transcriptome Sequencing of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells", "PRJNA688352", "Other", "A transcriptome analysis of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells", null, null, null, "WT 10d k2", "WT 10d k2", null, "strain:AB|isolate:missing|breed:missing|cultivar:missing|ecotype:missing|age:13 month|sex:not collected|tissue:kidney and headkidney|biomaterial provider:Southwest University  China|replicate:Biological replicate 2|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio: kidney and headkidney from  wild type and congenitally asplenic zebrafish post vaccination", "WT 10d k2", "WT 10d k2", "other", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "HiSeq X Ten", null, "SRP299583", null, null, "WT_10d_k2.raw_1.fastq.gz WT_10d_k2.raw_2.fastq.gz", "fastq fastq", 6354585300.0, 21181951.0, "WT 10d k2.raw 1.fastq.gz", "0:150 1:150", "A:1749380892;C:1406921465;G:1504331323;T:1693821680;N:129940", 150, 150, null, null, 1749380892, 1406921465, 1504331323, 1693821680, 129940, "SRX9747094", "SRS7934714", "SRA1178602", "southwest university|college of fisheries", "southwest university", 2, 0.93216, 0.9306, 0.0766, 0.07651, 0.70847, 0.70936, 0.53878, 0.53632, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "other", "unknown", "bulk", "unknown", "unknown", null, "China", "2020-12-28", "Adult", "Adult", "Kidney", "Renal System"], [62690, "SRR13319608", "SRX9747093", "SRS7934720", "SRP299583", "PRJNA688352", "Transcriptome Sequencing of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells", "PRJNA688352", "Other", "A transcriptome analysis of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells", null, null, null, "WT 10d k1", "WT 10d k1", null, "strain:AB|isolate:missing|breed:missing|cultivar:missing|ecotype:missing|age:12 month|sex:not collected|tissue:kidney and headkidney|biomaterial provider:Southwest University  China|replicate:Biological replicate 1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio: kidney and headkidney from  wild type and congenitally asplenic zebrafish post vaccination", "WT 10d k1", "WT 10d k1", "other", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "HiSeq X Ten", null, "SRP299583", null, null, "WT_10d_k1.raw_1.fastq.gz WT_10d_k1.raw_2.fastq.gz", "fastq fastq", 8712565800.0, 29041886.0, "WT 10d k1.raw 1.fastq.gz", "0:150 1:150", "A:2388117969;C:1934065753;G:2091791049;T:2298416148;N:174881", 150, 150, null, null, 2388117969, 1934065753, 2091791049, 2298416148, 174881, "SRX9747093", "SRS7934720", "SRA1178602", "southwest university|college of fisheries", "southwest university", 2, 0.93468, 0.93398, 0.09783, 0.0975, 0.69593, 0.69649, 0.52524, 0.52599, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "other", "unknown", "bulk", "unknown", "unknown", null, "China", "2020-12-28", "Adult", "Adult", "Kidney", "Renal System"], [62691, "SRR13319609", "SRX9747092", "SRS7934712", "SRP299583", "PRJNA688352", "Transcriptome Sequencing of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells", "PRJNA688352", "Other", "A transcriptome analysis of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells", null, null, null, "Mut 6h k3", "Mut 6h k3", null, "strain:AB|isolate:missing|breed:missing|cultivar:missing|ecotype:missing|age:11 month|sex:not collected|tissue:kidney and headkidney|biomaterial provider:Southwest University  China|replicate:Biological replicate 3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio: kidney and headkidney from  wild type and congenitally asplenic zebrafish post vaccination", "Mut 6h k3", "Mut 6h k3", "other", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "HiSeq X Ten", null, "SRP299583", null, null, "Mut_6h_k3.raw_1.fastq.gz Mut_6h_k3.raw_2.fastq.gz", "fastq fastq", 6821167500.0, 22737225.0, "Mut 6h k3.raw 1.fastq.gz", "0:150 1:150", "A:1903976437;C:1488858303;G:1566082857;T:1862227563;N:22340", 150, 150, null, null, 1903976437, 1488858303, 1566082857, 1862227563, 22340, "SRX9747092", "SRS7934712", "SRA1178602", "southwest university|college of fisheries", "southwest university", 2, 0.93422, 0.93209, 0.09882, 0.09902, 0.71784, 0.71892, 0.53469, 0.5464, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "other", "unknown", "bulk", "unknown", "unknown", null, "China", "2020-12-28", "Adult", "Adult", "Kidney", "Renal System"], [62692, "SRR13319610", "SRX9747091", "SRS7934717", "SRP299583", "PRJNA688352", "Transcriptome Sequencing of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells", "PRJNA688352", "Other", "A transcriptome analysis of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells", null, null, null, "Mut 6h k2", "Mut 6h k2", null, "strain:AB|isolate:missing|breed:missing|cultivar:missing|ecotype:missing|age:10 month|sex:not collected|tissue:kidney and headkidney|biomaterial provider:Southwest University  China|replicate:Biological replicate 2|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio: kidney and headkidney from  wild type and congenitally asplenic zebrafish post vaccination", "Mut 6h k2", "Mut 6h k2", "other", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "HiSeq X Ten", null, "SRP299583", null, null, "Mut_6h_k2.raw_1.fastq.gz Mut_6h_k2.raw_2.fastq.gz", "fastq fastq", 6614007900.0, 22046693.0, "Mut 6h k2.raw 1.fastq.gz", "0:150 1:150", "A:1836116506;C:1454221417;G:1524536821;T:1799111318;N:21838", 150, 150, null, null, 1836116506, 1454221417, 1524536821, 1799111318, 21838, "SRX9747091", "SRS7934717", "SRA1178602", "southwest university|college of fisheries", "southwest university", 2, 0.93405, 0.93165, 0.09928, 0.0998, 0.71378, 0.71405, 0.53405, 0.53636, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "other", "unknown", "bulk", "unknown", "unknown", null, "China", "2020-12-28", "Adult", "Adult", "Kidney", "Renal System"], [62693, "SRR13319611", "SRX9747090", "SRS7934716", "SRP299583", "PRJNA688352", "Transcriptome Sequencing of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells", "PRJNA688352", "Other", "A transcriptome analysis of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells", null, null, null, "Mut 6h k1", "Mut 6h k1", null, "strain:AB|isolate:missing|breed:missing|cultivar:missing|ecotype:missing|age:9 month|sex:not collected|tissue:kidney and headkidney|biomaterial provider:Southwest University  China|replicate:Biological replicate 1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio: kidney and headkidney from  wild type and congenitally asplenic zebrafish post vaccination", "Mut 6h k1", "Mut 6h k1", "other", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "HiSeq X Ten", null, "SRP299583", null, null, "Mut_6h_k1.raw_1.fastq.gz Mut_6h_k1.raw_2.fastq.gz", "fastq fastq", 6856096500.0, 22853655.0, "Mut 6h k1.raw 1.fastq.gz", "0:150 1:150", "A:1914687245;C:1494001561;G:1567738680;T:1879647930;N:21084", 150, 150, null, null, 1914687245, 1494001561, 1567738680, 1879647930, 21084, "SRX9747090", "SRS7934716", "SRA1178602", "southwest university|college of fisheries", "southwest university", 2, 0.92882, 0.92611, 0.10791, 0.10832, 0.71378, 0.71427, 0.53012, 0.52707, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "other", "unknown", "bulk", "unknown", "unknown", null, "China", "2020-12-28", "Adult", "Adult", "Kidney", "Renal System"], [62694, "SRR13319612", "SRX9747089", "SRS7934713", "SRP299583", "PRJNA688352", "Transcriptome Sequencing of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells", "PRJNA688352", "Other", "A transcriptome analysis of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells", null, null, null, "WT 6h k3", "WT 6h k3", null, "strain:AB|isolate:missing|breed:missing|cultivar:missing|ecotype:missing|age:8 month|sex:not collected|tissue:kidney and headkidney|biomaterial provider:Southwest University  China|replicate:Biological replicate 3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio: kidney and headkidney from  wild type and congenitally asplenic zebrafish post vaccination", "WT 6h k3", "WT 6h k3", "other", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "HiSeq X Ten", null, "SRP299583", null, null, "WT_6h_k3.raw_1.fastq.gz WT_6h_k3.raw_2.fastq.gz", "fastq fastq", 7194856500.0, 23982855.0, "WT 6h k3.raw 1.fastq.gz", "0:150 1:150", "A:2000223263;C:1580258499;G:1657803759;T:1956549208;N:21771", 150, 150, null, null, 2000223263, 1580258499, 1657803759, 1956549208, 21771, "SRX9747089", "SRS7934713", "SRA1178602", "southwest university|college of fisheries", "southwest university", 2, 0.93193, 0.92986, 0.09623, 0.09609, 0.70907, 0.71029, 0.53843, 0.53754, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "other", "unknown", "bulk", "unknown", "unknown", null, "China", "2020-12-28", "Adult", "Adult", "Kidney", "Renal System"], [62695, "SRR13319613", "SRX9747088", "SRS7934715", "SRP299583", "PRJNA688352", "Transcriptome Sequencing of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells", "PRJNA688352", "Other", "A transcriptome analysis of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells", null, null, null, "Mut 10d k3", "Mut 10d k3", null, "strain:AB|isolate:missing|breed:missing|cultivar:missing|ecotype:missing|age:17 month|sex:not collected|tissue:kidney and headkidney|biomaterial provider:Southwest University  China|replicate:Biological replicate 3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio: kidney and headkidney from  wild type and congenitally asplenic zebrafish post vaccination", "Mut 10d k3", "Mut 10d k3", "other", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "HiSeq X Ten", null, "SRP299583", null, null, "Mut_10d_k3.raw_1.fastq.gz Mut_10d_k3.raw_2.fastq.gz", "fastq fastq", 7859046900.0, 26196823.0, "Mut 10d k3.raw 1.fastq.gz", "0:150 1:150", "A:2162835249;C:1743162639;G:1857995098;T:2094896175;N:157739", 150, 150, null, null, 2162835249, 1743162639, 1857995098, 2094896175, 157739, "SRX9747088", "SRS7934715", "SRA1178602", "southwest university|college of fisheries", "southwest university", 2, 0.93831, 0.93732, 0.0836, 0.08331, 0.69988, 0.70132, 0.54542, 0.54383, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "other", "unknown", "bulk", "unknown", "unknown", null, "China", "2020-12-28", "Adult", "Adult", "Kidney", "Renal System"], [62696, "SRR13319614", "SRX9747087", "SRS7934710", "SRP299583", "PRJNA688352", "Transcriptome Sequencing of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells", "PRJNA688352", "Other", "A transcriptome analysis of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells", null, null, null, "Mut 10d k2", "Mut 10d k2", null, "strain:AB|isolate:missing|breed:missing|cultivar:missing|ecotype:missing|age:16 month|sex:not collected|tissue:kidney and headkidney|biomaterial provider:Southwest University  China|replicate:Biological replicate 2|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio: kidney and headkidney from  wild type and congenitally asplenic zebrafish post vaccination", "Mut 10d k2", "Mut 10d k2", "other", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "other", "PAIRED", "ILLUMINA", "HiSeq X Ten", null, "SRP299583", null, null, "Mut_10d_k2.raw_1.fastq.gz Mut_10d_k2.raw_2.fastq.gz", "fastq fastq", 6163552500.0, 20545175.0, "Mut 10d k2.raw 1.fastq.gz", "0:150 1:150", "A:1690284188;C:1371212067;G:1471613432;T:1630318376;N:124437", 150, 150, null, null, 1690284188, 1371212067, 1471613432, 1630318376, 124437, "SRX9747087", "SRS7934710", "SRA1178602", "southwest university|college of fisheries", "southwest university", 2, 0.94142, 0.93953, 0.07637, 0.07599, 0.7123, 0.71323, 0.54883, 0.55044, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "other", "unknown", "bulk", "unknown", "unknown", null, "China", "2020-12-28", "Adult", "Adult", "Kidney", "Renal System"], [62697, "SRR13319615", "SRX9747086", "SRS7934709", "SRP299583", "PRJNA688352", "Transcriptome Sequencing of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells", "PRJNA688352", "Other", "A transcriptome analysis of Kidneys in adult wild type and congenitally asplenic zebrafish post injected with inactivated A. hydrophila cells", null, null, null, "WT 6h k2", "WT 6h k2", null, "strain:AB|isolate:missing|breed:missing|cultivar:missing|ecotype:missing|age:7 month|sex:not collected|tissue:kidney and headkidney|biomaterial provider:Southwest University  China|replicate:Biological replicate 2|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio: kidney and headkidney from  wild type and congenitally asplenic zebrafish post vaccination", "WT 6h k2", "WT 6h k2", "other", null, 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