{"database": "metadata", "table": "run_metadata", "is_view": false, "human_description_en": "where experiment.library_selection = \"cDNA_oligo_dT\" and tissue_curation_coarse = \"Sensory System\"", "rows": [[33379, "SRR30184285", "SRX25650011", "SRS22293080", "SRP525334", "PRJNA1145997", "Regulation of optic nerve regeneration by srebf2", "PRJNA1145997", "Other", "Laser capture microdissection of the retinal ganglion cell layer followed by mRNA seq was used to measure differentially expressed genes in adult zebrafish at three days post optic nerve crush injury. Experimental groups include naive control  nonspecific morpholino control  morpholino mediated srebf2 gene knockdown  nonspecific morpholino control plus daily heat shock in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish  and morpholino mediated srebf2 gene knockdown in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish with daily heatshock. 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Veldman  Medical College of Wisconsin|treatment:heatshock srebf2MO 2|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "LCM seq of Danio rerio:retinal ganglion cell layer", "L26", "L26", "LCM seq  NucleoSpin RNA XS purified  ultra low input RNAseq at Novogene", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA_oligo_dT", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP525334", null, null, "L26_S144_L006_R2_001.fastq.gz L26_S144_L006_R1_001.fastq.gz", "fastq fastq", 17129465368.0, 56720084.0, "L26 S144 L006 R1 001.fastq.gz", "0:151 1:151", "A:4750698191;C:3734802838;G:3895289691;T:4746170435;N:2504213", 151, 151, null, null, 4750698191, 3734802838, 3895289691, 4746170435, 2504213, "SRX25650002", "SRS22293071", "SRA1943640", "Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy", "Medical College of Wisconsin", 2, 0.93654, 0.92792, 0.07609, 0.07622, 0.75061, 0.75357, 0.52583, 0.53272, 151, 151, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-08-09", "Adult", "Adult", "Eye", "Sensory System"], [33389, "SRR30184295", "SRX25650001", "SRS22293070", "SRP525334", "PRJNA1145997", "Regulation of optic nerve regeneration by srebf2", "PRJNA1145997", "Other", "Laser capture microdissection of the retinal ganglion cell layer followed by mRNA seq was used to measure differentially expressed genes in adult zebrafish at three days post optic nerve crush injury. Experimental groups include naive control  nonspecific morpholino control  morpholino mediated srebf2 gene knockdown  nonspecific morpholino control plus daily heat shock in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish  and morpholino mediated srebf2 gene knockdown in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish with daily heatshock. These data provide a resource to study the effects of srebf2 manipulation on transcriptomic responses to optic nerve injury in the adult zebrafish model.", null, null, null, "heatshock srebf2MO 1", "hs smo1 M", null, "strain:Tghsp70l:nSrebf2 2AmCherry  cmcl2:EGFP|age:>3 month|collection date:2023 11 06|geo loc name:USA: Milwaukee  WI|sex:male|tissue:retinal ganglion cell layer|biomaterial provider:Matthew B. Veldman  Medical College of Wisconsin|treatment:heatshock srebf2MO 1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "LCM seq of Danio rerio:retinal ganglion cell layer", "L25", "L25", "LCM seq  NucleoSpin RNA XS purified  ultra low input RNAseq at Novogene", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA_oligo_dT", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP525334", null, null, "L25_S114_L004_R2_001.fastq.gz L25_S114_L004_R1_001.fastq.gz", "fastq fastq", 18411870316.0, 60966458.0, "L25 S114 L004 R1 001.fastq.gz", "0:151 1:151", "A:5037571347;C:4144572498;G:4103153869;T:5124440844;N:2131758", 151, 151, null, null, 5037571347, 4144572498, 4103153869, 5124440844, 2131758, "SRX25650001", "SRS22293070", "SRA1943640", "Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy", "Medical College of Wisconsin", 2, 0.92822, 0.92792, 0.0886, 0.08918, 0.75974, 0.75978, 0.55638, 0.55618, 151, 151, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-08-09", "Adult", "Adult", "Eye", "Sensory System"], [33390, "SRR30184296", "SRX25650000", "SRS22293066", "SRP525334", "PRJNA1145997", "Regulation of optic nerve regeneration by srebf2", "PRJNA1145997", "Other", "Laser capture microdissection of the retinal ganglion cell layer followed by mRNA seq was used to measure differentially expressed genes in adult zebrafish at three days post optic nerve crush injury. Experimental groups include naive control  nonspecific morpholino control  morpholino mediated srebf2 gene knockdown  nonspecific morpholino control plus daily heat shock in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish  and morpholino mediated srebf2 gene knockdown in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish with daily heatshock. These data provide a resource to study the effects of srebf2 manipulation on transcriptomic responses to optic nerve injury in the adult zebrafish model.", null, null, null, "uninjured 3", "wt L1 F", null, "strain:wild type|age:>3 month|collection date:2023 11 06|geo loc name:USA: Milwaukee  WI|sex:female|tissue:retinal ganglion cell layer|biomaterial provider:Matthew B. Veldman  Medical College of Wisconsin|treatment:uninjured 3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "LCM seq of Danio rerio:retinal ganglion cell layer", "L03", "L03", "LCM seq  NucleoSpin RNA XS purified  ultra low input RNAseq at Novogene", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA_oligo_dT", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP525334", null, null, "L03_S105_L004_R1_001.fastq.gz L03_S105_L004_R2_001.fastq.gz", "fastq fastq", 19718182792.0, 65291996.0, "L03 S105 L004 R1 001.fastq.gz", "0:151 1:151", "A:5247234351;C:4504410887;G:4526181297;T:5438074144;N:2282113", 151, 151, null, null, 5247234351, 4504410887, 4526181297, 5438074144, 2282113, "SRX25650000", "SRS22293066", "SRA1943640", "Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy", "Medical College of Wisconsin", 2, 0.90475, 0.90699, 0.11305, 0.11331, 0.75014, 0.74929, 0.52801, 0.54316, 151, 151, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-08-09", "Adult", "Adult", "Eye", "Sensory System"], [33391, "SRR30184297", "SRX25649999", "SRS22293069", "SRP525334", "PRJNA1145997", "Regulation of optic nerve regeneration by srebf2", "PRJNA1145997", "Other", "Laser capture microdissection of the retinal ganglion cell layer followed by mRNA seq was used to measure differentially expressed genes in adult zebrafish at three days post optic nerve crush injury. Experimental groups include naive control  nonspecific morpholino control  morpholino mediated srebf2 gene knockdown  nonspecific morpholino control plus daily heat shock in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish  and morpholino mediated srebf2 gene knockdown in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish with daily heatshock. These data provide a resource to study the effects of srebf2 manipulation on transcriptomic responses to optic nerve injury in the adult zebrafish model.", null, null, null, "heatshock cMO 4", "hs cmo2 F", null, "strain:Tghsp70l:nSrebf2 2AmCherry  cmcl2:EGFP|age:>3 month|collection date:2023 11 06|geo loc name:USA: Milwaukee  WI|sex:female|tissue:retinal ganglion cell layer|biomaterial provider:Matthew B. Veldman  Medical College of Wisconsin|treatment:heatshock cMO 4|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "LCM seq of Danio rerio:retinal ganglion cell layer", "L24", "L24", "LCM seq  NucleoSpin RNA XS purified  ultra low input RNAseq at Novogene", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA_oligo_dT", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP525334", null, null, "L24_S134_L005_R2_001.fastq.gz L24_S134_L005_R1_001.fastq.gz", "fastq fastq", 19377661484.0, 64164442.0, "L24 S134 L005 R1 001.fastq.gz", "0:151 1:151", "A:5371834087;C:4284931090;G:4227428342;T:5492109103;N:1358862", 151, 151, null, null, 5371834087, 4284931090, 4227428342, 5492109103, 1358862, "SRX25649999", "SRS22293069", "SRA1943640", "Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy", "Medical College of Wisconsin", 2, 0.9104, 0.90922, 0.11083, 0.11062, 0.75325, 0.7543, 0.54602, 0.54303, 151, 151, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-08-09", "Adult", "Adult", "Eye", "Sensory System"], [33392, "SRR30184298", "SRX25649998", "SRS22293067", "SRP525334", "PRJNA1145997", "Regulation of optic nerve regeneration by srebf2", "PRJNA1145997", "Other", "Laser capture microdissection of the retinal ganglion cell layer followed by mRNA seq was used to measure differentially expressed genes in adult zebrafish at three days post optic nerve crush injury. Experimental groups include naive control  nonspecific morpholino control  morpholino mediated srebf2 gene knockdown  nonspecific morpholino control plus daily heat shock in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish  and morpholino mediated srebf2 gene knockdown in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish with daily heatshock. These data provide a resource to study the effects of srebf2 manipulation on transcriptomic responses to optic nerve injury in the adult zebrafish model.", null, null, null, "heatshock cMO 3", "hs cmo1 F", null, "strain:Tghsp70l:nSrebf2 2AmCherry  cmcl2:EGFP|age:>3 month|collection date:2023 11 06|geo loc name:USA: Milwaukee  WI|sex:female|tissue:retinal ganglion cell layer|biomaterial provider:Matthew B. Veldman  Medical College of Wisconsin|treatment:heatshock cMO 3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "LCM seq of Danio rerio:retinal ganglion cell layer", "L23", "L23", "LCM seq  NucleoSpin RNA XS purified  ultra low input RNAseq at Novogene", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA_oligo_dT", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP525334", null, null, "L23_S143_L006_R2_001.fastq.gz L23_S143_L006_R1_001.fastq.gz", "fastq fastq", 28079439956.0, 92978278.0, "L23 S143 L006 R1 001.fastq.gz", "0:151 1:151", "A:7811720040;C:6084170964;G:6064272363;T:8115079585;N:4197004", 151, 151, null, null, 7811720040, 6084170964, 6064272363, 8115079585, 4197004, "SRX25649998", "SRS22293067", "SRA1943640", "Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy", "Medical College of Wisconsin", 2, 0.86593, 0.86525, 0.14584, 0.14587, 0.77498, 0.77433, 0.59534, 0.59433, 151, 151, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-08-09", "Adult", "Adult", "Eye", "Sensory System"], [33393, "SRR30184299", "SRX25649997", "SRS22293068", "SRP525334", "PRJNA1145997", "Regulation of optic nerve regeneration by srebf2", "PRJNA1145997", "Other", "Laser capture microdissection of the retinal ganglion cell layer followed by mRNA seq was used to measure differentially expressed genes in adult zebrafish at three days post optic nerve crush injury. Experimental groups include naive control  nonspecific morpholino control  morpholino mediated srebf2 gene knockdown  nonspecific morpholino control plus daily heat shock in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish  and morpholino mediated srebf2 gene knockdown in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish with daily heatshock. These data provide a resource to study the effects of srebf2 manipulation on transcriptomic responses to optic nerve injury in the adult zebrafish model.", null, null, null, "heatshock cMO 2", "hs cmo2 M", null, "strain:Tghsp70l:nSrebf2 2AmCherry  cmcl2:EGFP|age:>3 month|collection date:2023 11 06|geo loc name:USA: Milwaukee  WI|sex:male|tissue:retinal ganglion cell layer|biomaterial provider:Matthew B. Veldman  Medical College of Wisconsin|treatment:heatshock cMO 2|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "LCM seq of Danio rerio:retinal ganglion cell layer", "L22", "L22", "LCM seq  NucleoSpin RNA XS purified  ultra low input RNAseq at Novogene", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA_oligo_dT", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP525334", null, null, "L22_S133_L005_R2_001.fastq.gz L22_S133_L005_R1_001.fastq.gz", "fastq fastq", 20494382588.0, 67862194.0, "L22 S133 L005 R1 001.fastq.gz", "0:151 1:151", "A:5610165229;C:4583590395;G:4590759271;T:5708435247;N:1432446", 151, 151, null, null, 5610165229, 4583590395, 4590759271, 5708435247, 1432446, "SRX25649997", "SRS22293068", "SRA1943640", "Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy", "Medical College of Wisconsin", 2, 0.92306, 0.92279, 0.0879, 0.08765, 0.7671, 0.76579, 0.56402, 0.56401, 151, 151, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-08-09", "Adult", "Adult", "Eye", "Sensory System"], [33394, "SRR30184300", "SRX25649996", "SRS22293064", "SRP525334", "PRJNA1145997", "Regulation of optic nerve regeneration by srebf2", "PRJNA1145997", "Other", "Laser capture microdissection of the retinal ganglion cell layer followed by mRNA seq was used to measure differentially expressed genes in adult zebrafish at three days post optic nerve crush injury. Experimental groups include naive control  nonspecific morpholino control  morpholino mediated srebf2 gene knockdown  nonspecific morpholino control plus daily heat shock in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish  and morpholino mediated srebf2 gene knockdown in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish with daily heatshock. These data provide a resource to study the effects of srebf2 manipulation on transcriptomic responses to optic nerve injury in the adult zebrafish model.", null, null, null, "heatshock cMO 1", "hs cmo1 M", null, "strain:Tghsp70l:nSrebf2 2AmCherry  cmcl2:EGFP|age:>3 month|collection date:2023 11 06|geo loc name:USA: Milwaukee  WI|sex:male|tissue:retinal ganglion cell layer|biomaterial provider:Matthew B. Veldman  Medical College of Wisconsin|treatment:heatshock cMO 1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "LCM seq of Danio rerio:retinal ganglion cell layer", "L21", "L21", "LCM seq  NucleoSpin RNA XS purified  ultra low input RNAseq at Novogene", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA_oligo_dT", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP525334", null, null, "L21_S113_L004_R1_001.fastq.gz L21_S113_L004_R2_001.fastq.gz", "fastq fastq", 22282038670.0, 73781585.0, "L21 S113 L004 R1 001.fastq.gz", "0:151 1:151", "A:5984432885;C:5103219794;G:5123288852;T:6068507910;N:2589229", 151, 151, null, null, 5984432885, 5103219794, 5123288852, 6068507910, 2589229, "SRX25649996", "SRS22293064", "SRA1943640", "Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy", "Medical College of Wisconsin", 2, 0.93523, 0.93442, 0.06557, 0.06558, 0.76463, 0.76532, 0.54365, 0.53711, 151, 151, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-08-09", "Adult", "Adult", "Eye", "Sensory System"], [33395, "SRR30184301", "SRX25649995", "SRS22293061", "SRP525334", "PRJNA1145997", "Regulation of optic nerve regeneration by srebf2", "PRJNA1145997", "Other", "Laser capture microdissection of the retinal ganglion cell layer followed by mRNA seq was used to measure differentially expressed genes in adult zebrafish at three days post optic nerve crush injury. Experimental groups include naive control  nonspecific morpholino control  morpholino mediated srebf2 gene knockdown  nonspecific morpholino control plus daily heat shock in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish  and morpholino mediated srebf2 gene knockdown in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish with daily heatshock. These data provide a resource to study the effects of srebf2 manipulation on transcriptomic responses to optic nerve injury in the adult zebrafish model.", null, null, null, "srebf2MO 4", "wt smo2 F", null, "strain:wild type|age:>3 month|collection date:2023 11 06|geo loc name:USA: Milwaukee  WI|sex:female|tissue:retinal ganglion cell layer|biomaterial provider:Matthew B. Veldman  Medical College of Wisconsin|treatment:srebf2MO 4|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "LCM seq of Danio rerio:retinal ganglion cell layer", "L16", "L16", "LCM seq  NucleoSpin RNA XS purified  ultra low input RNAseq at Novogene", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA_oligo_dT", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP525334", null, null, "L16_S108_L004_R1_001.fastq.gz L16_S108_L004_R2_001.fastq.gz", "fastq fastq", 25364244630.0, 83987565.0, "L16 S108 L004 R1 001.fastq.gz", "0:151 1:151", "A:6783811492;C:5825885841;G:5791188806;T:6960421955;N:2936536", 151, 151, null, null, 6783811492, 5825885841, 5791188806, 6960421955, 2936536, "SRX25649995", "SRS22293061", "SRA1943640", "Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy", "Medical College of Wisconsin", 2, 0.93174, 0.93131, 0.06871, 0.06898, 0.75461, 0.75386, 0.54103, 0.54073, 151, 151, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-08-09", "Adult", "Adult", "Eye", "Sensory System"], [33396, "SRR30184302", "SRX25649994", "SRS22293065", "SRP525334", "PRJNA1145997", "Regulation of optic nerve regeneration by srebf2", "PRJNA1145997", "Other", "Laser capture microdissection of the retinal ganglion cell layer followed by mRNA seq was used to measure differentially expressed genes in adult zebrafish at three days post optic nerve crush injury. Experimental groups include naive control  nonspecific morpholino control  morpholino mediated srebf2 gene knockdown  nonspecific morpholino control plus daily heat shock in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish  and morpholino mediated srebf2 gene knockdown in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish with daily heatshock. These data provide a resource to study the effects of srebf2 manipulation on transcriptomic responses to optic nerve injury in the adult zebrafish model.", null, null, null, "srebf2MO 3", "wt smo1 F", null, "strain:wild type|age:>3 month|collection date:2023 11 06|geo loc name:USA: Milwaukee  WI|sex:female|tissue:retinal ganglion cell layer|biomaterial provider:Matthew B. Veldman  Medical College of Wisconsin|treatment:srebf2MO 3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "LCM seq of Danio rerio:retinal ganglion cell layer", "L15", "L15", "LCM seq  NucleoSpin RNA XS purified  ultra low input RNAseq at Novogene", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA_oligo_dT", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP525334", null, null, "L15_S132_L005_R2_001.fastq.gz L15_S132_L005_R1_001.fastq.gz", "fastq fastq", 24643120876.0, 81599738.0, "L15 S132 L005 R1 001.fastq.gz", "0:151 1:151", "A:6791560596;C:5441282614;G:5412453327;T:6996084050;N:1740289", 151, 151, null, null, 6791560596, 5441282614, 5412453327, 6996084050, 1740289, "SRX25649994", "SRS22293065", "SRA1943640", "Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy", "Medical College of Wisconsin", 2, 0.90806, 0.90874, 0.11049, 0.11033, 0.75885, 0.75741, 0.54345, 0.55215, 151, 151, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-08-09", "Adult", "Adult", "Eye", "Sensory System"], [33397, "SRR30184303", "SRX25649993", "SRS22293062", "SRP525334", "PRJNA1145997", "Regulation of optic nerve regeneration by srebf2", "PRJNA1145997", "Other", "Laser capture microdissection of the retinal ganglion cell layer followed by mRNA seq was used to measure differentially expressed genes in adult zebrafish at three days post optic nerve crush injury. Experimental groups include naive control  nonspecific morpholino control  morpholino mediated srebf2 gene knockdown  nonspecific morpholino control plus daily heat shock in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish  and morpholino mediated srebf2 gene knockdown in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish with daily heatshock. These data provide a resource to study the effects of srebf2 manipulation on transcriptomic responses to optic nerve injury in the adult zebrafish model.", null, null, null, "srebf2MO 2", "wt smo2 M", null, "strain:wild type|age:>3 month|collection date:2023 11 06|geo loc name:USA: Milwaukee  WI|sex:male|tissue:retinal ganglion cell layer|biomaterial provider:Matthew B. Veldman  Medical College of Wisconsin|treatment:srebf2MO 2|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "LCM seq of Danio rerio:retinal ganglion cell layer", "L14", "L14", "LCM seq  NucleoSpin RNA XS purified  ultra low input RNAseq at Novogene", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA_oligo_dT", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP525334", null, null, "L14_S131_L005_R2_001.fastq.gz L14_S131_L005_R1_001.fastq.gz", "fastq fastq", 20984328060.0, 69484530.0, "L14 S131 L005 R1 001.fastq.gz", "0:151 1:151", "A:5728503785;C:4710613293;G:4687638412;T:5856111147;N:1461423", 151, 151, null, null, 5728503785, 4710613293, 4687638412, 5856111147, 1461423, "SRX25649993", "SRS22293062", "SRA1943640", "Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy", "Medical College of Wisconsin", 2, 0.92516, 0.92471, 0.08416, 0.08435, 0.76568, 0.76585, 0.56286, 0.56146, 151, 151, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-08-09", "Adult", "Adult", "Eye", "Sensory System"], [33398, "SRR30184304", "SRX25649992", "SRS22293059", "SRP525334", "PRJNA1145997", "Regulation of optic nerve regeneration by srebf2", "PRJNA1145997", "Other", "Laser capture microdissection of the retinal ganglion cell layer followed by mRNA seq was used to measure differentially expressed genes in adult zebrafish at three days post optic nerve crush injury. Experimental groups include naive control  nonspecific morpholino control  morpholino mediated srebf2 gene knockdown  nonspecific morpholino control plus daily heat shock in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish  and morpholino mediated srebf2 gene knockdown in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish with daily heatshock. These data provide a resource to study the effects of srebf2 manipulation on transcriptomic responses to optic nerve injury in the adult zebrafish model.", null, null, null, "srebf2MO 1", "wt smo1 M", null, "strain:wild type|age:>3 month|collection date:2023 11 06|geo loc name:USA: Milwaukee  WI|sex:male|tissue:retinal ganglion cell layer|biomaterial provider:Matthew B. 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Experimental groups include naive control  nonspecific morpholino control  morpholino mediated srebf2 gene knockdown  nonspecific morpholino control plus daily heat shock in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish  and morpholino mediated srebf2 gene knockdown in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish with daily heatshock. These data provide a resource to study the effects of srebf2 manipulation on transcriptomic responses to optic nerve injury in the adult zebrafish model.", null, null, null, "uninjured 1", "wt L1 M", null, "strain:wild type|age:>3 month|collection date:2023 11 06|geo loc name:USA: Milwaukee  WI|sex:male|tissue:retinal ganglion cell layer|biomaterial provider:Matthew B. 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