{"database": "metadata", "table": "run_metadata", "is_view": false, "human_description_en": "where experiment.library_selection = \"cDNA_oligo_dT\"", "rows": [[30691, "SRR28270998", "SRX23880961", "SRS20704477", "SRP494117", "PRJNA1085662", "Roxithromycin exposure induces motoneuron malformation and behavioral deficits of zebrafish by interfering with the differentiation of motor neuron progenitor cells", "PRJNA1085662", "Other", "Roxithromycin ROX  a commonly used macrolide antibiotic  is extensively employed in human medicine and livestock industries. Due to its structural stability and resistance to biological degradation  ROX persists as a resilient environmental contaminant  detectable in aquatic ecosystems and food products. However  our understanding of the potential health risks to humans from continuous ROX exposure remains limited. In this study  we used the zebrafish as a vertebrate model to explore the potential developmental toxicity of early ROX exposure  particularly focusing on its effects on locomotor functionality and motoneuron development. Early exposure to ROX induces marked developmental toxicity in zebrafish embryos  significantly reducing hatch rates  body lengths  and increased malformation rates. Moreover  ROX exposure adversely affected the locomotive capacity of zebrafish embryos  and observations in transgenic zebrafish Tghb9:eGFP revealed axonal loss in motor neurons  evident through reduced or irregular axonal lengths. Concurrently  abnormal apoptosis in ROX exposed zebrafish embryos intensified alongside the upregulation of apoptosis related genes bax  bcl2  caspase 3a. Single cell sequencing further disclosed substantial effects of ROX on genes involved in the differentiation of motor neuron progenitor cells ngn1  olig2  axon development cd82a  mbpa  plp1b  sema5a  and neuroimmunity aplnrb  aplnra in zebrafish larvae. Furthermore  the motor neuron defects induced by ROX can be rescued by administering ngn1 agonist. In summary  ROX exposure leads to early life abnormalities in zebrafish motor neurons and locomotor behavior by hindering the differentiation of motor neuron progenitor cells and inducing abnormal apoptosis.", null, null, null, null, "WT", null, "strain:not provided|isolate:not provided|breed:not provided|cultivar:not provided|ecotype:not provided|age:not provided|dev stage:not provided|collection date:not provided|geo loc name:not provided|sex:not provided|tissue:Cerebrum|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Roxithromycin exposure induces mot1uron malformation and behavioral deficits of zebrafish by interfering with the differentiation of motor neuron progenitor cells", "DANIO", "DANIO", "Illumina Second Generation Sequencing", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA_oligo_dT", "SINGLE", "BGISEQ", "BGISEQ-500", null, "SRP494117", null, null, "WT_S1_L001_I1_001.fastq.gz", "fastq", 7991376264.0, 998922033.0, "WT S1 L001 I1 001.fastq.gz", "0:8", "A:2573734851;C:1426474966;G:1492966198;T:2498173074;N:27175", 8, null, null, null, 2573734851, 1426474966, 1492966198, 2498173074, 27175, "SRX23880961", "SRS20704477", "SRA1820072", "shantou university|Neurobiology Center", "shantou university", 1, 0.0, null, 0.0, null, 1.0, null, null, null, 8, null, "T", null, "under 1.2% mapping rate", "bgi", "bgi", "unknown", "poly_a", "unknown", "sc_generic", "single_cell_generic", "generic-scrnaseq-only", null, "China", "2024-03-11", "Undetermined", "Multi-stage", "Brain", "Nervous System"], [32107, "SRR29012733", "SRX24539435", "SRS21284312", "SRP507393", "PRJNA1111146", "RNA seq of adult zebrafish brain following traumatic brain injury and/or ISRIB exposure", "PRJNA1111146", "Other", "Here analysed gene expression changes post Traumatic Brain Injury TBI and subsequent exposure to ISRIB in the brain of adult Danio rerio. 4 groups are available: control C  traumatic brain injury TBI; T  ISRIB injection I TBI and ISRIB injection TI.", null, null, null, null, "I3 TK13", null, "strain:Wild type|age:5 month|collection date:2023 08 20|geo loc name:Russia|sex:NA|tissue:brain|sample type:I3 TK13|treatment:ISRIB|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Here analysed gene expression changes post Traumatic Brain Injury TBI and subsequent exposure to ISRIB in the brain of adult Danio rerio. 4 groups are available: control C  traumatic brain injury TBI; T  ISRIB injection I TBI and ISRIB injection TI.", "TK13", "TK13", "RNA isolation was performed using the ExtractRNA reagent following the standard protocol for TriZol mediated extraction. The RNA quality was checked by capillary electrophoresis using the QIAxcel Advanced program. Total RNA was used for isolation of polyA fraction using NEBNext PolyA mRNA Magnetic Isolation Module according to the manufacturer's recommendations. RNA was next quantified on Quntus fluorometer and used for library preparation using NEBNext UltraTM Directional RNA Library PrepKit for Illumina with NEBNext Multiplex Oligos for Illumina according to the manufacturer's recommendations. The quality of libraries was confirmed by capillary electrophoresis using the QIAxcel Advanced program. Pair end sequencing was performed using Illumina HiSeq2500 with a read length of 110 bp.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA_oligo_dT", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP507393", null, null, "I3-TK13_S9_R1_001.fastq.gz I3-TK13_S9_R2_001.fastq.gz", "fastq fastq", 1951476351.0, 9046948.0, "I3 TK13 S9 R1 001.fastq.gz", "0:107.84 1:107.86", "A:532508211;C:436986899;G:434983140;T:539541026;N:7457075", 107, 107, null, null, 532508211, 436986899, 434983140, 539541026, 7457075, "SRX24539435", "SRS21284312", "SRA1865594", "Sirius University of Science and Technology|Genetics and Life Science", "Sirius University of Science and Technology", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "nebnext", "bulk", "unknown", "unknown", null, "Russia", "2024-05-13", "Adult", "Adult", "Brain", "Nervous System"], [32108, "SRR29012734", "SRX24539434", "SRS21284311", "SRP507393", "PRJNA1111146", "RNA seq of adult zebrafish brain following traumatic brain injury and/or ISRIB exposure", "PRJNA1111146", "Other", "Here analysed gene expression changes post Traumatic Brain Injury TBI and subsequent exposure to ISRIB in the brain of adult Danio rerio. 4 groups are available: control C  traumatic brain injury TBI; T  ISRIB injection I TBI and ISRIB injection TI.", null, null, null, null, "I2 TK12", null, "strain:Wild type|age:5 month|collection date:2023 08 20|geo loc name:Russia|sex:NA|tissue:brain|sample type:I2 TK12|treatment:ISRIB|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Here analysed gene expression changes post Traumatic Brain Injury TBI and subsequent exposure to ISRIB in the brain of adult Danio rerio. 4 groups are available: control C  traumatic brain injury TBI; T  ISRIB injection I TBI and ISRIB injection TI.", "TK12", "TK12", "RNA isolation was performed using the ExtractRNA reagent following the standard protocol for TriZol mediated extraction. The RNA quality was checked by capillary electrophoresis using the QIAxcel Advanced program. Total RNA was used for isolation of polyA fraction using NEBNext PolyA mRNA Magnetic Isolation Module according to the manufacturer's recommendations. RNA was next quantified on Quntus fluorometer and used for library preparation using NEBNext UltraTM Directional RNA Library PrepKit for Illumina with NEBNext Multiplex Oligos for Illumina according to the manufacturer's recommendations. The quality of libraries was confirmed by capillary electrophoresis using the QIAxcel Advanced program. Pair end sequencing was performed using Illumina HiSeq2500 with a read length of 110 bp.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA_oligo_dT", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP507393", null, null, "I2-TK12_S8_R1_001.fastq.gz I2-TK12_S8_R2_001.fastq.gz", "fastq fastq", 2483693754.0, 11573280.0, "I2 TK12 S8 R1 001.fastq.gz", "0:107.28 1:107.32", "A:690681797;C:542407183;G:537633561;T:698259696;N:14711517", 107, 107, null, null, 690681797, 542407183, 537633561, 698259696, 14711517, "SRX24539434", "SRS21284311", "SRA1865594", "Sirius University of Science and Technology|Genetics and Life Science", "Sirius University of Science and Technology", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "nebnext", "bulk", "unknown", "unknown", null, "Russia", "2024-05-13", "Adult", "Adult", "Brain", "Nervous System"], [32109, "SRR29012735", "SRX24539433", "SRS21284310", "SRP507393", "PRJNA1111146", "RNA seq of adult zebrafish brain following traumatic brain injury and/or ISRIB exposure", "PRJNA1111146", "Other", "Here analysed gene expression changes post Traumatic Brain Injury TBI and subsequent exposure to ISRIB in the brain of adult Danio rerio. 4 groups are available: control C  traumatic brain injury TBI; T  ISRIB injection I TBI and ISRIB injection TI.", null, null, null, null, "C9 TK39", null, "strain:Wild type|age:5 month|collection date:2023 08 20|geo loc name:Russia|sex:NA|tissue:brain|sample type:C9 TK39|treatment:Control|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Here analysed gene expression changes post Traumatic Brain Injury TBI and subsequent exposure to ISRIB in the brain of adult Danio rerio. 4 groups are available: control C  traumatic brain injury TBI; T  ISRIB injection I TBI and ISRIB injection TI.", "TK39", "TK39", "RNA isolation was performed using the ExtractRNA reagent following the standard protocol for TriZol mediated extraction. The RNA quality was checked by capillary electrophoresis using the QIAxcel Advanced program. Total RNA was used for isolation of polyA fraction using NEBNext PolyA mRNA Magnetic Isolation Module according to the manufacturer's recommendations. RNA was next quantified on Quntus fluorometer and used for library preparation using NEBNext UltraTM Directional RNA Library PrepKit for Illumina with NEBNext Multiplex Oligos for Illumina according to the manufacturer's recommendations. The quality of libraries was confirmed by capillary electrophoresis using the QIAxcel Advanced program. Pair end sequencing was performed using Illumina HiSeq2500 with a read length of 110 bp.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA_oligo_dT", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP507393", null, null, "C9-TK39_S27_R1_001.fastq.gz C9-TK39_S27_R2_001.fastq.gz", "fastq fastq", 2370365419.0, 11054395.0, "C9 TK39 S27 R1 001.fastq.gz", "0:107.19 1:107.24", "A:638716678;C:538512113;G:535138614;T:642111655;N:15886359", 107, 107, null, null, 638716678, 538512113, 535138614, 642111655, 15886359, "SRX24539433", "SRS21284310", "SRA1865594", "Sirius University of Science and Technology|Genetics and Life Science", "Sirius University of Science and Technology", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "nebnext", "bulk", "unknown", "unknown", null, "Russia", "2024-05-13", "Adult", "Adult", "Brain", "Nervous System"], [32110, "SRR29012736", "SRX24539432", "SRS21284309", "SRP507393", "PRJNA1111146", "RNA seq of adult zebrafish brain following traumatic brain injury and/or ISRIB exposure", "PRJNA1111146", "Other", "Here analysed gene expression changes post Traumatic Brain Injury TBI and subsequent exposure to ISRIB in the brain of adult Danio rerio. 4 groups are available: control C  traumatic brain injury TBI; T  ISRIB injection I TBI and ISRIB injection TI.", null, null, null, null, "C8 TK38", null, "strain:Wild type|age:5 month|collection date:2023 08 20|geo loc name:Russia|sex:NA|tissue:brain|sample type:C8 TK38|treatment:Control|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Here analysed gene expression changes post Traumatic Brain Injury TBI and subsequent exposure to ISRIB in the brain of adult Danio rerio. 4 groups are available: control C  traumatic brain injury TBI; T  ISRIB injection I TBI and ISRIB injection TI.", "TK38", "TK38", "RNA isolation was performed using the ExtractRNA reagent following the standard protocol for TriZol mediated extraction. The RNA quality was checked by capillary electrophoresis using the QIAxcel Advanced program. Total RNA was used for isolation of polyA fraction using NEBNext PolyA mRNA Magnetic Isolation Module according to the manufacturer's recommendations. RNA was next quantified on Quntus fluorometer and used for library preparation using NEBNext UltraTM Directional RNA Library PrepKit for Illumina with NEBNext Multiplex Oligos for Illumina according to the manufacturer's recommendations. The quality of libraries was confirmed by capillary electrophoresis using the QIAxcel Advanced program. Pair end sequencing was performed using Illumina HiSeq2500 with a read length of 110 bp.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA_oligo_dT", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP507393", null, null, "C8-TK38_S26_R1_001.fastq.gz C8-TK38_S26_R2_001.fastq.gz", "fastq fastq", 2329920345.0, 10848811.0, "C8 TK38 S26 R1 001.fastq.gz", "0:107.36 1:107.40", "A:628958466;C:528866941;G:524557104;T:634765152;N:12772682", 107, 107, null, null, 628958466, 528866941, 524557104, 634765152, 12772682, "SRX24539432", "SRS21284309", "SRA1865594", "Sirius University of Science and Technology|Genetics and Life Science", "Sirius University of Science and Technology", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "nebnext", "bulk", "unknown", "unknown", null, "Russia", "2024-05-13", "Adult", "Adult", "Brain", "Nervous System"], [32111, "SRR29012737", "SRX24539431", "SRS21284308", "SRP507393", "PRJNA1111146", "RNA seq of adult zebrafish brain following traumatic brain injury and/or ISRIB exposure", "PRJNA1111146", "Other", "Here analysed gene expression changes post Traumatic Brain Injury TBI and subsequent exposure to ISRIB in the brain of adult Danio rerio. 4 groups are available: control C  traumatic brain injury TBI; T  ISRIB injection I TBI and ISRIB injection TI.", null, null, null, null, "C7 TK37", null, "strain:Wild type|age:5 month|collection date:2023 08 20|geo loc name:Russia|sex:NA|tissue:brain|sample type:C7 TK37|treatment:Control|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Here analysed gene expression changes post Traumatic Brain Injury TBI and subsequent exposure to ISRIB in the brain of adult Danio rerio. 4 groups are available: control C  traumatic brain injury TBI; T  ISRIB injection I TBI and ISRIB injection TI.", "TK37", "TK37", "RNA isolation was performed using the ExtractRNA reagent following the standard protocol for TriZol mediated extraction. The RNA quality was checked by capillary electrophoresis using the QIAxcel Advanced program. Total RNA was used for isolation of polyA fraction using NEBNext PolyA mRNA Magnetic Isolation Module according to the manufacturer's recommendations. RNA was next quantified on Quntus fluorometer and used for library preparation using NEBNext UltraTM Directional RNA Library PrepKit for Illumina with NEBNext Multiplex Oligos for Illumina according to the manufacturer's recommendations. The quality of libraries was confirmed by capillary electrophoresis using the QIAxcel Advanced program. Pair end sequencing was performed using Illumina HiSeq2500 with a read length of 110 bp.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA_oligo_dT", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP507393", null, null, "C7-TK37_S25_R1_001.fastq.gz C7-TK37_S25_R2_001.fastq.gz", "fastq fastq", 1536459611.0, 7103135.0, "C7 TK37 S25 R1 001.fastq.gz", "0:108.14 1:108.16", "A:423368949;C:341783029;G:338600069;T:427083273;N:5624291", 108, 108, null, null, 423368949, 341783029, 338600069, 427083273, 5624291, "SRX24539431", "SRS21284308", "SRA1865594", "Sirius University of Science and Technology|Genetics and Life Science", "Sirius University of Science and Technology", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "nebnext", "bulk", "unknown", "unknown", null, "Russia", "2024-05-13", "Adult", "Adult", "Brain", "Nervous System"], [32112, "SRR29012738", "SRX24539430", "SRS21284307", "SRP507393", "PRJNA1111146", "RNA seq of adult zebrafish brain following traumatic brain injury and/or ISRIB exposure", "PRJNA1111146", "Other", "Here analysed gene expression changes post Traumatic Brain Injury TBI and subsequent exposure to ISRIB in the brain of adult Danio rerio. 4 groups are available: control C  traumatic brain injury TBI; T  ISRIB injection I TBI and ISRIB injection TI.", null, null, null, null, "C5 TK35", null, "strain:Wild type|age:5 month|collection date:2023 08 20|geo loc name:Russia|sex:NA|tissue:brain|sample type:C5 TK35|treatment:Control|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Here analysed gene expression changes post Traumatic Brain Injury TBI and subsequent exposure to ISRIB in the brain of adult Danio rerio. 4 groups are available: control C  traumatic brain injury TBI; T  ISRIB injection I TBI and ISRIB injection TI.", "TK35", "TK35", "RNA isolation was performed using the ExtractRNA reagent following the standard protocol for TriZol mediated extraction. The RNA quality was checked by capillary electrophoresis using the QIAxcel Advanced program. Total RNA was used for isolation of polyA fraction using NEBNext PolyA mRNA Magnetic Isolation Module according to the manufacturer's recommendations. RNA was next quantified on Quntus fluorometer and used for library preparation using NEBNext UltraTM Directional RNA Library PrepKit for Illumina with NEBNext Multiplex Oligos for Illumina according to the manufacturer's recommendations. The quality of libraries was confirmed by capillary electrophoresis using the QIAxcel Advanced program. Pair end sequencing was performed using Illumina HiSeq2500 with a read length of 110 bp.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA_oligo_dT", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP507393", null, null, "C5-TK35_S24_R1_001.fastq.gz C5-TK35_S24_R2_001.fastq.gz", "fastq fastq", 2315322327.0, 10788443.0, "C5 TK35 S24 R1 001.fastq.gz", "0:107.29 1:107.33", "A:634261894;C:516670403;G:512433057;T:639505411;N:12451562", 107, 107, null, null, 634261894, 516670403, 512433057, 639505411, 12451562, "SRX24539430", "SRS21284307", "SRA1865594", "Sirius University of Science and Technology|Genetics and Life Science", "Sirius University of Science and Technology", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "nebnext", "bulk", "unknown", "unknown", null, "Russia", "2024-05-13", "Adult", "Adult", "Brain", "Nervous System"], [32113, "SRR29012739", "SRX24539429", "SRS21284306", "SRP507393", "PRJNA1111146", "RNA seq of adult zebrafish brain following traumatic brain injury and/or ISRIB exposure", "PRJNA1111146", "Other", "Here analysed gene expression changes post Traumatic Brain Injury TBI and subsequent exposure to ISRIB in the brain of adult Danio rerio. 4 groups are available: control C  traumatic brain injury TBI; T  ISRIB injection I TBI and ISRIB injection TI.", null, null, null, null, "C4 TK34", null, "strain:Wild type|age:5 month|collection date:2023 08 20|geo loc name:Russia|sex:NA|tissue:brain|sample type:C4 TK34|treatment:Control|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Here analysed gene expression changes post Traumatic Brain Injury TBI and subsequent exposure to ISRIB in the brain of adult Danio rerio. 4 groups are available: control C  traumatic brain injury TBI; T  ISRIB injection I TBI and ISRIB injection TI.", "TK34", "TK34", "RNA isolation was performed using the ExtractRNA reagent following the standard protocol for TriZol mediated extraction. The RNA quality was checked by capillary electrophoresis using the QIAxcel Advanced program. Total RNA was used for isolation of polyA fraction using NEBNext PolyA mRNA Magnetic Isolation Module according to the manufacturer's recommendations. RNA was next quantified on Quntus fluorometer and used for library preparation using NEBNext UltraTM Directional RNA Library PrepKit for Illumina with NEBNext Multiplex Oligos for Illumina according to the manufacturer's recommendations. The quality of libraries was confirmed by capillary electrophoresis using the QIAxcel Advanced program. Pair end sequencing was performed using Illumina HiSeq2500 with a read length of 110 bp.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA_oligo_dT", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP507393", null, null, "C4-TK34_S23_R1_001.fastq.gz C4-TK34_S23_R2_001.fastq.gz", "fastq fastq", 2329888357.0, 11027349.0, "C4 TK34 S23 R1 001.fastq.gz", "0:105.60 1:105.68", "A:630397662;C:520059288;G:515161552;T:635983184;N:28286671", 105, 105, null, null, 630397662, 520059288, 515161552, 635983184, 28286671, "SRX24539429", "SRS21284306", "SRA1865594", "Sirius University of Science and Technology|Genetics and Life Science", "Sirius University of Science and Technology", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "nebnext", "bulk", "unknown", "unknown", null, "Russia", "2024-05-13", "Adult", "Adult", "Brain", "Nervous System"], [32114, "SRR29012740", "SRX24539428", "SRS21284305", "SRP507393", "PRJNA1111146", "RNA seq of adult zebrafish brain following traumatic brain injury and/or ISRIB exposure", "PRJNA1111146", "Other", "Here analysed gene expression changes post Traumatic Brain Injury TBI and subsequent exposure to ISRIB in the brain of adult Danio rerio. 4 groups are available: control C  traumatic brain injury TBI; T  ISRIB injection I TBI and ISRIB injection TI.", null, null, null, null, "TI8 TK8", null, "strain:Wild type|age:5 month|collection date:2023 08 20|geo loc name:Russia|sex:NA|tissue:brain|sample type:TI8 TK8|treatment:TBI + ISRIB|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Here analysed gene expression changes post Traumatic Brain Injury TBI and subsequent exposure to ISRIB in the brain of adult Danio rerio. 4 groups are available: control C  traumatic brain injury TBI; T  ISRIB injection I TBI and ISRIB injection TI.", "TK8", "TK8", "RNA isolation was performed using the ExtractRNA reagent following the standard protocol for TriZol mediated extraction. The RNA quality was checked by capillary electrophoresis using the QIAxcel Advanced program. Total RNA was used for isolation of polyA fraction using NEBNext PolyA mRNA Magnetic Isolation Module according to the manufacturer's recommendations. RNA was next quantified on Quntus fluorometer and used for library preparation using NEBNext UltraTM Directional RNA Library PrepKit for Illumina with NEBNext Multiplex Oligos for Illumina according to the manufacturer's recommendations. The quality of libraries was confirmed by capillary electrophoresis using the QIAxcel Advanced program. Pair end sequencing was performed using Illumina HiSeq2500 with a read length of 110 bp.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA_oligo_dT", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP507393", null, null, "TI8-TK8_S7_R1_001.fastq.gz TI8-TK8_S7_R2_001.fastq.gz", "fastq fastq", 4277303861.0, 19878893.0, "TI8 TK8 S7 R1 001.fastq.gz", "0:107.58 1:107.59", "A:1152512692;C:974435535;G:968603233;T:1168971271;N:12781130", 107, 107, null, null, 1152512692, 974435535, 968603233, 1168971271, 12781130, "SRX24539428", "SRS21284305", "SRA1865594", "Sirius University of Science and Technology|Genetics and Life Science", "Sirius University of Science and Technology", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "nebnext", "bulk", "unknown", "unknown", null, "Russia", "2024-05-13", "Adult", "Adult", "Brain", "Nervous System"], [32115, "SRR29012741", "SRX24539427", "SRS21284304", "SRP507393", "PRJNA1111146", "RNA seq of adult zebrafish brain following traumatic brain injury and/or ISRIB exposure", "PRJNA1111146", "Other", "Here analysed gene expression changes post Traumatic Brain Injury TBI and subsequent exposure to ISRIB in the brain of adult Danio rerio. 4 groups are available: control C  traumatic brain injury TBI; T  ISRIB injection I TBI and ISRIB injection TI.", null, null, null, null, "TI7 TK7", null, "strain:Wild type|age:5 month|collection date:2023 08 20|geo loc name:Russia|sex:NA|tissue:brain|sample type:TI7 TK7|treatment:TBI + ISRIB|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Here analysed gene expression changes post Traumatic Brain Injury TBI and subsequent exposure to ISRIB in the brain of adult Danio rerio. 4 groups are available: control C  traumatic brain injury TBI; T  ISRIB injection I TBI and ISRIB injection TI.", "TK7", "TK7", "RNA isolation was performed using the ExtractRNA reagent following the standard protocol for TriZol mediated extraction. The RNA quality was checked by capillary electrophoresis using the QIAxcel Advanced program. Total RNA was used for isolation of polyA fraction using NEBNext PolyA mRNA Magnetic Isolation Module according to the manufacturer's recommendations. RNA was next quantified on Quntus fluorometer and used for library preparation using NEBNext UltraTM Directional RNA Library PrepKit for Illumina with NEBNext Multiplex Oligos for Illumina according to the manufacturer's recommendations. The quality of libraries was confirmed by capillary electrophoresis using the QIAxcel Advanced program. Pair end sequencing was performed using Illumina HiSeq2500 with a read length of 110 bp.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA_oligo_dT", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP507393", null, null, "TI7-TK7_S6_R1_001.fastq.gz TI7-TK7_S6_R2_001.fastq.gz", "fastq fastq", 2617012040.0, 12126551.0, "TI7 TK7 S6 R1 001.fastq.gz", "0:107.90 1:107.91", "A:701588599;C:599589501;G:599522057;T:710050024;N:6261859", 107, 107, null, null, 701588599, 599589501, 599522057, 710050024, 6261859, "SRX24539427", "SRS21284304", "SRA1865594", "Sirius University of Science and Technology|Genetics and Life Science", "Sirius University of Science and Technology", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "nebnext", "bulk", "unknown", "unknown", null, "Russia", "2024-05-13", "Adult", "Adult", "Brain", "Nervous System"], [32116, "SRR29012742", "SRX24539426", "SRS21284303", "SRP507393", "PRJNA1111146", "RNA seq of adult zebrafish brain following traumatic brain injury and/or ISRIB exposure", "PRJNA1111146", "Other", "Here analysed gene expression changes post Traumatic Brain Injury TBI and subsequent exposure to ISRIB in the brain of adult Danio rerio. 4 groups are available: control C  traumatic brain injury TBI; T  ISRIB injection I TBI and ISRIB injection TI.", null, null, null, null, "TI6 TK6", null, "strain:Wild type|age:5 month|collection date:2023 08 20|geo loc name:Russia|sex:NA|tissue:brain|sample type:TI6 TK6|treatment:TBI + ISRIB|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Here analysed gene expression changes post Traumatic Brain Injury TBI and subsequent exposure to ISRIB in the brain of adult Danio rerio. 4 groups are available: control C  traumatic brain injury TBI; T  ISRIB injection I TBI and ISRIB injection TI.", "TK6", "TK6", "RNA isolation was performed using the ExtractRNA reagent following the standard protocol for TriZol mediated extraction. The RNA quality was checked by capillary electrophoresis using the QIAxcel Advanced program. Total RNA was used for isolation of polyA fraction using NEBNext PolyA mRNA Magnetic Isolation Module according to the manufacturer's recommendations. RNA was next quantified on Quntus fluorometer and used for library preparation using NEBNext UltraTM Directional RNA Library PrepKit for Illumina with NEBNext Multiplex Oligos for Illumina according to the manufacturer's recommendations. The quality of libraries was confirmed by capillary electrophoresis using the QIAxcel Advanced program. Pair end sequencing was performed using Illumina HiSeq2500 with a read length of 110 bp.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA_oligo_dT", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP507393", null, null, "TI6-TK6_S5_R1_001.fastq.gz TI6-TK6_S5_R2_001.fastq.gz", "fastq fastq", 5193201377.0, 24181964.0, "TI6 TK6 S5 R1 001.fastq.gz", "0:107.36 1:107.40", "A:1427289221;C:1151717905;G:1140736875;T:1447664955;N:25792421", 107, 107, null, null, 1427289221, 1151717905, 1140736875, 1447664955, 25792421, "SRX24539426", "SRS21284303", "SRA1865594", "Sirius University of Science and Technology|Genetics and Life Science", "Sirius University of Science and Technology", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "nebnext", "bulk", "unknown", "unknown", null, "Russia", "2024-05-13", "Adult", "Adult", "Brain", "Nervous System"], [32117, "SRR29012743", "SRX24539425", "SRS21284302", "SRP507393", "PRJNA1111146", "RNA seq of adult zebrafish brain following traumatic brain injury and/or ISRIB exposure", "PRJNA1111146", "Other", "Here analysed gene expression changes post Traumatic Brain Injury TBI and subsequent exposure to ISRIB in the brain of adult Danio rerio. 4 groups are available: control C  traumatic brain injury TBI; T  ISRIB injection I TBI and ISRIB injection TI.", null, null, null, null, "TI5 TK5", null, "strain:Wild type|age:5 month|collection date:2023 08 20|geo loc name:Russia|sex:NA|tissue:brain|sample type:TI5 TK5|treatment:TBI + ISRIB|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Here analysed gene expression changes post Traumatic Brain Injury TBI and subsequent exposure to ISRIB in the brain of adult Danio rerio. 4 groups are available: control C  traumatic brain injury TBI; T  ISRIB injection I TBI and ISRIB injection TI.", "TK5", "TK5", "RNA isolation was performed using the ExtractRNA reagent following the standard protocol for TriZol mediated extraction. The RNA quality was checked by capillary electrophoresis using the QIAxcel Advanced program. Total RNA was used for isolation of polyA fraction using NEBNext PolyA mRNA Magnetic Isolation Module according to the manufacturer's recommendations. RNA was next quantified on Quntus fluorometer and used for library preparation using NEBNext UltraTM Directional RNA Library PrepKit for Illumina with NEBNext Multiplex Oligos for Illumina according to the manufacturer's recommendations. The quality of libraries was confirmed by capillary electrophoresis using the QIAxcel Advanced program. Pair end sequencing was performed using Illumina HiSeq2500 with a read length of 110 bp.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA_oligo_dT", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP507393", null, null, "TI5-TK5_S4_R1_001.fastq.gz TI5-TK5_S4_R2_001.fastq.gz", "fastq fastq", 2048648256.0, 9524761.0, "TI5 TK5 S4 R1 001.fastq.gz", "0:107.53 1:107.56", "A:575845206;C:442981520;G:439368910;T:583602331;N:6850289", 107, 107, null, null, 575845206, 442981520, 439368910, 583602331, 6850289, "SRX24539425", "SRS21284302", "SRA1865594", "Sirius University of Science and Technology|Genetics and Life Science", "Sirius University of Science and Technology", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "nebnext", "bulk", "unknown", "unknown", null, "Russia", "2024-05-13", "Adult", "Adult", "Brain", "Nervous System"], [32118, "SRR29012744", "SRX24539424", "SRS21284301", "SRP507393", "PRJNA1111146", "RNA seq of adult zebrafish brain following traumatic brain injury and/or ISRIB exposure", "PRJNA1111146", "Other", "Here analysed gene expression changes post Traumatic Brain Injury TBI and subsequent exposure to ISRIB in the brain of adult Danio rerio. 4 groups are available: control C  traumatic brain injury TBI; T  ISRIB injection I TBI and ISRIB injection TI.", null, null, null, null, "TI4 TK4", null, "strain:Wild type|age:5 month|collection date:2023 08 20|geo loc name:Russia|sex:NA|tissue:brain|sample type:TI4 TK4|treatment:TBI + ISRIB|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Here analysed gene expression changes post Traumatic Brain Injury TBI and subsequent exposure to ISRIB in the brain of adult Danio rerio. 4 groups are available: control C  traumatic brain injury TBI; T  ISRIB injection I TBI and ISRIB injection TI.", "TK4", "TK4", "RNA isolation was performed using the ExtractRNA reagent following the standard protocol for TriZol mediated extraction. The RNA quality was checked by capillary electrophoresis using the QIAxcel Advanced program. Total RNA was used for isolation of polyA fraction using NEBNext PolyA mRNA Magnetic Isolation Module according to the manufacturer's recommendations. RNA was next quantified on Quntus fluorometer and used for library preparation using NEBNext UltraTM Directional RNA Library PrepKit for Illumina with NEBNext Multiplex Oligos for Illumina according to the manufacturer's recommendations. The quality of libraries was confirmed by capillary electrophoresis using the QIAxcel Advanced program. Pair end sequencing was performed using Illumina HiSeq2500 with a read length of 110 bp.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA_oligo_dT", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP507393", null, null, "TI4-TK4_S3_R1_001.fastq.gz TI4-TK4_S3_R2_001.fastq.gz", "fastq fastq", 2858158305.0, 13330760.0, "TI4 TK4 S3 R1 001.fastq.gz", "0:107.18 1:107.22", "A:781642776;C:636257123;G:631858904;T:791480839;N:16918663", 107, 107, null, null, 781642776, 636257123, 631858904, 791480839, 16918663, "SRX24539424", "SRS21284301", "SRA1865594", "Sirius University of Science and Technology|Genetics and Life Science", "Sirius University of Science and Technology", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "nebnext", "bulk", "unknown", "unknown", null, "Russia", "2024-05-13", "Adult", "Adult", "Brain", "Nervous System"], [32119, "SRR29012745", "SRX24539423", "SRS21284300", "SRP507393", "PRJNA1111146", "RNA seq of adult zebrafish brain following traumatic brain injury and/or ISRIB exposure", "PRJNA1111146", "Other", "Here analysed gene expression changes post Traumatic Brain Injury TBI and subsequent exposure to ISRIB in the brain of adult Danio rerio. 4 groups are available: control C  traumatic brain injury TBI; T  ISRIB injection I TBI and ISRIB injection TI.", null, null, null, null, "TI3 TK3", null, "strain:Wild type|age:5 month|collection date:2023 08 20|geo loc name:Russia|sex:NA|tissue:brain|sample type:TI3 TK3|treatment:TBI + ISRIB|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Here analysed gene expression changes post Traumatic Brain Injury TBI and subsequent exposure to ISRIB in the brain of adult Danio rerio. 4 groups are available: control C  traumatic brain injury TBI; T  ISRIB injection I TBI and ISRIB injection TI.", "TK3", "TK3", "RNA isolation was performed using the ExtractRNA reagent following the standard protocol for TriZol mediated extraction. The RNA quality was checked by capillary electrophoresis using the QIAxcel Advanced program. Total RNA was used for isolation of polyA fraction using NEBNext PolyA mRNA Magnetic Isolation Module according to the manufacturer's recommendations. RNA was next quantified on Quntus fluorometer and used for library preparation using NEBNext UltraTM Directional RNA Library PrepKit for Illumina with NEBNext Multiplex Oligos for Illumina according to the manufacturer's recommendations. The quality of libraries was confirmed by capillary electrophoresis using the QIAxcel Advanced program. Pair end sequencing was performed using Illumina HiSeq2500 with a read length of 110 bp.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA_oligo_dT", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP507393", null, null, "TI3-TK3_S2_R1_001.fastq.gz TI3-TK3_S2_R2_001.fastq.gz", "fastq fastq", 1566054292.0, 7297460.0, "TI3 TK3 S2 R1 001.fastq.gz", "0:107.29 1:107.31", "A:419497793;C:357426293;G:355453976;T:425654356;N:8021874", 107, 107, null, null, 419497793, 357426293, 355453976, 425654356, 8021874, "SRX24539423", "SRS21284300", "SRA1865594", "Sirius University of Science and Technology|Genetics and Life Science", "Sirius University of Science and Technology", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "nebnext", "bulk", "unknown", "unknown", null, "Russia", "2024-05-13", "Adult", "Adult", "Brain", "Nervous System"], [32120, "SRR29012746", "SRX24539422", "SRS21284299", "SRP507393", "PRJNA1111146", "RNA seq of adult zebrafish brain following traumatic brain injury and/or ISRIB exposure", "PRJNA1111146", "Other", "Here analysed gene expression changes post Traumatic Brain Injury TBI and subsequent exposure to ISRIB in the brain of adult Danio rerio. 4 groups are available: control C  traumatic brain injury TBI; T  ISRIB injection I TBI and ISRIB injection TI.", null, null, null, null, "TI2 TK2", null, "strain:Wild type|age:5 month|collection date:2023 08 20|geo loc name:Russia|sex:NA|tissue:brain|sample type:TI2 TK2|treatment:TBI + ISRIB|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Here analysed gene expression changes post Traumatic Brain Injury TBI and subsequent exposure to ISRIB in the brain of adult Danio rerio. 4 groups are available: control C  traumatic brain injury TBI; T  ISRIB injection I TBI and ISRIB injection TI.", "TK2", "TK2", "RNA isolation was performed using the ExtractRNA reagent following the standard protocol for TriZol mediated extraction. The RNA quality was checked by capillary electrophoresis using the QIAxcel Advanced program. Total RNA was used for isolation of polyA fraction using NEBNext PolyA mRNA Magnetic Isolation Module according to the manufacturer's recommendations. RNA was next quantified on Quntus fluorometer and used for library preparation using NEBNext UltraTM Directional RNA Library PrepKit for Illumina with NEBNext Multiplex Oligos for Illumina according to the manufacturer's recommendations. The quality of libraries was confirmed by capillary electrophoresis using the QIAxcel Advanced program. Pair end sequencing was performed using Illumina HiSeq2500 with a read length of 110 bp.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA_oligo_dT", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP507393", null, null, "TI2-TK2_S1_R1_001.fastq.gz TI2-TK2_S1_R2_001.fastq.gz", "fastq fastq", 1865518160.0, 8666289.0, "TI2 TK2 S1 R1 001.fastq.gz", "0:107.62 1:107.64", "A:510590407;C:415852163;G:413264662;T:517549106;N:8261822", 107, 107, null, null, 510590407, 415852163, 413264662, 517549106, 8261822, "SRX24539422", "SRS21284299", "SRA1865594", "Sirius University of Science and Technology|Genetics and Life Science", "Sirius University of Science and Technology", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "nebnext", "bulk", "unknown", "unknown", null, "Russia", "2024-05-13", "Adult", "Adult", "Brain", "Nervous System"], [32121, "SRR29012747", "SRX24539421", "SRS21284297", "SRP507393", "PRJNA1111146", "RNA seq of adult zebrafish brain following traumatic brain injury and/or ISRIB exposure", "PRJNA1111146", "Other", "Here analysed gene expression changes post Traumatic Brain Injury TBI and subsequent exposure to ISRIB in the brain of adult Danio rerio. 4 groups are available: control C  traumatic brain injury TBI; T  ISRIB injection I TBI and ISRIB injection TI.", null, null, null, null, "C3 TK33", null, "strain:Wild type|age:5 month|collection date:2023 08 20|geo loc name:Russia|sex:NA|tissue:brain|sample type:C3 TK33|treatment:Control|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Here analysed gene expression changes post Traumatic Brain Injury TBI and subsequent exposure to ISRIB in the brain of adult Danio rerio. 4 groups are available: control C  traumatic brain injury TBI; T  ISRIB injection I TBI and ISRIB injection TI.", "TK33", "TK33", "RNA isolation was performed using the ExtractRNA reagent following the standard protocol for TriZol mediated extraction. The RNA quality was checked by capillary electrophoresis using the QIAxcel Advanced program. Total RNA was used for isolation of polyA fraction using NEBNext PolyA mRNA Magnetic Isolation Module according to the manufacturer's recommendations. RNA was next quantified on Quntus fluorometer and used for library preparation using NEBNext UltraTM Directional RNA Library PrepKit for Illumina with NEBNext Multiplex Oligos for Illumina according to the manufacturer's recommendations. The quality of libraries was confirmed by capillary electrophoresis using the QIAxcel Advanced program. Pair end sequencing was performed using Illumina HiSeq2500 with a read length of 110 bp.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA_oligo_dT", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP507393", null, null, "C3-TK33_S22_R1_001.fastq.gz C3-TK33_S22_R2_001.fastq.gz", "fastq fastq", 1780541368.0, 8253847.0, "C3 TK33 S22 R1 001.fastq.gz", "0:107.85 1:107.87", "A:513337117;C:371411358;G:365394452;T:521921928;N:8476513", 107, 107, null, null, 513337117, 371411358, 365394452, 521921928, 8476513, "SRX24539421", "SRS21284297", "SRA1865594", "Sirius University of Science and Technology|Genetics and Life Science", "Sirius University of Science and Technology", null, null, null, null, null, null, null, null, null, null, null, "T", "T", "mates < 9% mapping rate", "illumina", "hiseq_era", "unknown", "poly_a", "nebnext", "bulk", "unknown", "unknown", null, "Russia", "2024-05-13", "Adult", "Adult", "Brain", "Nervous System"], [32122, "SRR29012748", "SRX24539420", "SRS21284298", "SRP507393", "PRJNA1111146", "RNA seq of adult zebrafish brain following traumatic brain injury and/or ISRIB exposure", "PRJNA1111146", "Other", "Here analysed gene expression changes post Traumatic Brain Injury TBI and subsequent exposure to ISRIB in the brain of adult Danio rerio. 4 groups are available: control C  traumatic brain injury TBI; T  ISRIB injection I TBI and ISRIB injection TI.", null, null, null, null, "T7 TK30", null, "strain:Wild type|age:5 month|collection date:2023 08 20|geo loc name:Russia|sex:NA|tissue:brain|sample type:T7 TK30|treatment:TBI|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Here analysed gene expression changes post Traumatic Brain Injury TBI and subsequent exposure to ISRIB in the brain of adult Danio rerio. 4 groups are available: control C  traumatic brain injury TBI; T  ISRIB injection I TBI and ISRIB injection TI.", "TK30", "TK30", "RNA isolation was performed using the ExtractRNA reagent following the standard protocol for TriZol mediated extraction. The RNA quality was checked by capillary electrophoresis using the QIAxcel Advanced program. Total RNA was used for isolation of polyA fraction using NEBNext PolyA mRNA Magnetic Isolation Module according to the manufacturer's recommendations. RNA was next quantified on Quntus fluorometer and used for library preparation using NEBNext UltraTM Directional RNA Library PrepKit for Illumina with NEBNext Multiplex Oligos for Illumina according to the manufacturer's recommendations. The quality of libraries was confirmed by capillary electrophoresis using the QIAxcel Advanced program. Pair end sequencing was performed using Illumina HiSeq2500 with a read length of 110 bp.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA_oligo_dT", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP507393", null, null, "T7-TK30_S19_R1_001.fastq.gz T7-TK30_S19_R2_001.fastq.gz", "fastq fastq", 1503037534.0, 7034919.0, "T7 TK30 S19 R1 001.fastq.gz", "0:106.80 1:106.85", "A:415333842;C:329932770;G:326603495;T:418836802;N:12330625", 106, 106, null, null, 415333842, 329932770, 326603495, 418836802, 12330625, "SRX24539420", "SRS21284298", "SRA1865594", "Sirius University of Science and Technology|Genetics and Life Science", "Sirius University of Science and Technology", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "nebnext", "bulk", "unknown", "unknown", null, "Russia", "2024-05-13", "Adult", "Adult", "Brain", "Nervous System"], [32123, "SRR29012749", "SRX24539419", "SRS21284296", "SRP507393", "PRJNA1111146", "RNA seq of adult zebrafish brain following traumatic brain injury and/or ISRIB exposure", "PRJNA1111146", "Other", "Here analysed gene expression changes post Traumatic Brain Injury TBI and subsequent exposure to ISRIB in the brain of adult Danio rerio. 4 groups are available: control C  traumatic brain injury TBI; T  ISRIB injection I TBI and ISRIB injection TI.", null, null, null, null, "T6 TK29", null, "strain:Wild type|age:5 month|collection date:2023 08 20|geo loc name:Russia|sex:NA|tissue:brain|sample type:T6 TK29|treatment:TBI|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Here analysed gene expression changes post Traumatic Brain Injury TBI and subsequent exposure to ISRIB in the brain of adult Danio rerio. 4 groups are available: control C  traumatic brain injury TBI; T  ISRIB injection I TBI and ISRIB injection TI.", "TK29", "TK29", "RNA isolation was performed using the ExtractRNA reagent following the standard protocol for TriZol mediated extraction. The RNA quality was checked by capillary electrophoresis using the QIAxcel Advanced program. Total RNA was used for isolation of polyA fraction using NEBNext PolyA mRNA Magnetic Isolation Module according to the manufacturer's recommendations. RNA was next quantified on Quntus fluorometer and used for library preparation using NEBNext UltraTM Directional RNA Library PrepKit for Illumina with NEBNext Multiplex Oligos for Illumina according to the manufacturer's recommendations. The quality of libraries was confirmed by capillary electrophoresis using the QIAxcel Advanced program. Pair end sequencing was performed using Illumina HiSeq2500 with a read length of 110 bp.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA_oligo_dT", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP507393", null, null, "T6-TK29_S18_R1_001.fastq.gz T6-TK29_S18_R2_001.fastq.gz", "fastq fastq", 2167995268.0, 10034734.0, "T6 TK29 S18 R1 001.fastq.gz", "0:108.03 1:108.02", "A:579041799;C:499138909;G:494478121;T:585849580;N:9486859", 108, 108, null, null, 579041799, 499138909, 494478121, 585849580, 9486859, "SRX24539419", "SRS21284296", "SRA1865594", "Sirius University of Science and Technology|Genetics and Life Science", "Sirius University of Science and Technology", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "nebnext", "bulk", "unknown", "unknown", null, "Russia", "2024-05-13", "Adult", "Adult", "Brain", "Nervous System"], [32124, "SRR29012750", "SRX24539418", "SRS21284295", "SRP507393", "PRJNA1111146", "RNA seq of adult zebrafish brain following traumatic brain injury and/or ISRIB exposure", "PRJNA1111146", "Other", "Here analysed gene expression changes post Traumatic Brain Injury TBI and subsequent exposure to ISRIB in the brain of adult Danio rerio. 4 groups are available: control C  traumatic brain injury TBI; T  ISRIB injection I TBI and ISRIB injection TI.", null, null, null, null, "T5 TK28", null, "strain:Wild type|age:5 month|collection date:2023 08 20|geo loc name:Russia|sex:NA|tissue:brain|sample type:T5 TK28|treatment:TBI|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Here analysed gene expression changes post Traumatic Brain Injury TBI and subsequent exposure to ISRIB in the brain of adult Danio rerio. 4 groups are available: control C  traumatic brain injury TBI; T  ISRIB injection I TBI and ISRIB injection TI.", "TK28", "TK28", "RNA isolation was performed using the ExtractRNA reagent following the standard protocol for TriZol mediated extraction. The RNA quality was checked by capillary electrophoresis using the QIAxcel Advanced program. Total RNA was used for isolation of polyA fraction using NEBNext PolyA mRNA Magnetic Isolation Module according to the manufacturer's recommendations. RNA was next quantified on Quntus fluorometer and used for library preparation using NEBNext UltraTM Directional RNA Library PrepKit for Illumina with NEBNext Multiplex Oligos for Illumina according to the manufacturer's recommendations. The quality of libraries was confirmed by capillary electrophoresis using the QIAxcel Advanced program. Pair end sequencing was performed using Illumina HiSeq2500 with a read length of 110 bp.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA_oligo_dT", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP507393", null, null, "T5-TK28_S17_R1_001.fastq.gz T5-TK28_S17_R2_001.fastq.gz", "fastq fastq", 2809544566.0, 13129662.0, "T5 TK28 S17 R1 001.fastq.gz", "0:106.97 1:107.01", "A:778118767;C:619618875;G:614045445;T:781068333;N:16693146", 106, 107, null, null, 778118767, 619618875, 614045445, 781068333, 16693146, "SRX24539418", "SRS21284295", "SRA1865594", "Sirius University of Science and Technology|Genetics and Life Science", "Sirius University of Science and Technology", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "nebnext", "bulk", "unknown", "unknown", null, "Russia", "2024-05-13", "Adult", "Adult", "Brain", "Nervous System"], [32125, "SRR29012751", "SRX24539417", "SRS21284294", "SRP507393", "PRJNA1111146", "RNA seq of adult zebrafish brain following traumatic brain injury and/or ISRIB exposure", "PRJNA1111146", "Other", "Here analysed gene expression changes post Traumatic Brain Injury TBI and subsequent exposure to ISRIB in the brain of adult Danio rerio. 4 groups are available: control C  traumatic brain injury TBI; T  ISRIB injection I TBI and ISRIB injection TI.", null, null, null, null, "T4 TK27", null, "strain:Wild type|age:5 month|collection date:2023 08 20|geo loc name:Russia|sex:NA|tissue:brain|sample type:T4 TK27|treatment:TBI|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Here analysed gene expression changes post Traumatic Brain Injury TBI and subsequent exposure to ISRIB in the brain of adult Danio rerio. 4 groups are available: control C  traumatic brain injury TBI; T  ISRIB injection I TBI and ISRIB injection TI.", "TK27", "TK27", "RNA isolation was performed using the ExtractRNA reagent following the standard protocol for TriZol mediated extraction. The RNA quality was checked by capillary electrophoresis using the QIAxcel Advanced program. Total RNA was used for isolation of polyA fraction using NEBNext PolyA mRNA Magnetic Isolation Module according to the manufacturer's recommendations. RNA was next quantified on Quntus fluorometer and used for library preparation using NEBNext UltraTM Directional RNA Library PrepKit for Illumina with NEBNext Multiplex Oligos for Illumina according to the manufacturer's recommendations. The quality of libraries was confirmed by capillary electrophoresis using the QIAxcel Advanced program. Pair end sequencing was performed using Illumina HiSeq2500 with a read length of 110 bp.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA_oligo_dT", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP507393", null, null, "T4-TK27_S16_R1_001.fastq.gz T4-TK27_S16_R2_001.fastq.gz", "fastq fastq", 2225863101.0, 10367681.0, "T4 TK27 S16 R1 001.fastq.gz", "0:107.33 1:107.36", "A:603151281;C:502052154;G:496942138;T:608675289;N:15042239", 107, 107, null, null, 603151281, 502052154, 496942138, 608675289, 15042239, "SRX24539417", "SRS21284294", "SRA1865594", "Sirius University of Science and Technology|Genetics and Life Science", "Sirius University of Science and Technology", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "nebnext", "bulk", "unknown", "unknown", null, "Russia", "2024-05-13", "Adult", "Adult", "Brain", "Nervous System"], [32126, "SRR29012752", "SRX24539416", "SRS21284293", "SRP507393", "PRJNA1111146", "RNA seq of adult zebrafish brain following traumatic brain injury and/or ISRIB exposure", "PRJNA1111146", "Other", "Here analysed gene expression changes post Traumatic Brain Injury TBI and subsequent exposure to ISRIB in the brain of adult Danio rerio. 4 groups are available: control C  traumatic brain injury TBI; T  ISRIB injection I TBI and ISRIB injection TI.", null, null, null, null, "T3 TK26", null, "strain:Wild type|age:5 month|collection date:2023 08 20|geo loc name:Russia|sex:NA|tissue:brain|sample type:T3 TK26|treatment:TBI|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Here analysed gene expression changes post Traumatic Brain Injury TBI and subsequent exposure to ISRIB in the brain of adult Danio rerio. 4 groups are available: control C  traumatic brain injury TBI; T  ISRIB injection I TBI and ISRIB injection TI.", "TK26", "TK26", "RNA isolation was performed using the ExtractRNA reagent following the standard protocol for TriZol mediated extraction. The RNA quality was checked by capillary electrophoresis using the QIAxcel Advanced program. Total RNA was used for isolation of polyA fraction using NEBNext PolyA mRNA Magnetic Isolation Module according to the manufacturer's recommendations. RNA was next quantified on Quntus fluorometer and used for library preparation using NEBNext UltraTM Directional RNA Library PrepKit for Illumina with NEBNext Multiplex Oligos for Illumina according to the manufacturer's recommendations. The quality of libraries was confirmed by capillary electrophoresis using the QIAxcel Advanced program. Pair end sequencing was performed using Illumina HiSeq2500 with a read length of 110 bp.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA_oligo_dT", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP507393", null, null, "T3-TK26_S15_R1_001.fastq.gz T3-TK26_S15_R2_001.fastq.gz", "fastq fastq", 1940446267.0, 9166557.0, "T3 TK26 S15 R1 001.fastq.gz", "0:105.81 1:105.87", "A:525291529;C:433681588;G:429227771;T:530538742;N:21706637", 105, 105, null, null, 525291529, 433681588, 429227771, 530538742, 21706637, "SRX24539416", "SRS21284293", "SRA1865594", "Sirius University of Science and Technology|Genetics and Life Science", "Sirius University of Science and Technology", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "nebnext", "bulk", "unknown", "unknown", null, "Russia", "2024-05-13", "Adult", "Adult", "Brain", "Nervous System"], [32127, "SRR29012753", "SRX24539415", "SRS21284292", "SRP507393", "PRJNA1111146", "RNA seq of adult zebrafish brain following traumatic brain injury and/or ISRIB exposure", "PRJNA1111146", "Other", "Here analysed gene expression changes post Traumatic Brain Injury TBI and subsequent exposure to ISRIB in the brain of adult Danio rerio. 4 groups are available: control C  traumatic brain injury TBI; T  ISRIB injection I TBI and ISRIB injection TI.", null, null, null, null, "T2 TK23", null, "strain:Wild type|age:5 month|collection date:2023 08 20|geo loc name:Russia|sex:NA|tissue:brain|sample type:T2 TK23|treatment:TBI|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Here analysed gene expression changes post Traumatic Brain Injury TBI and subsequent exposure to ISRIB in the brain of adult Danio rerio. 4 groups are available: control C  traumatic brain injury TBI; T  ISRIB injection I TBI and ISRIB injection TI.", "TK23", "TK23", "RNA isolation was performed using the ExtractRNA reagent following the standard protocol for TriZol mediated extraction. The RNA quality was checked by capillary electrophoresis using the QIAxcel Advanced program. Total RNA was used for isolation of polyA fraction using NEBNext PolyA mRNA Magnetic Isolation Module according to the manufacturer's recommendations. RNA was next quantified on Quntus fluorometer and used for library preparation using NEBNext UltraTM Directional RNA Library PrepKit for Illumina with NEBNext Multiplex Oligos for Illumina according to the manufacturer's recommendations. The quality of libraries was confirmed by capillary electrophoresis using the QIAxcel Advanced program. Pair end sequencing was performed using Illumina HiSeq2500 with a read length of 110 bp.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA_oligo_dT", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP507393", null, null, "T2-TK22_S13_R1_001.fastq.gz T2-TK22_S13_R2_001.fastq.gz", "fastq fastq", 1732117888.0, 8020234.0, "T2 TK22 S13 R1 001.fastq.gz", "0:107.97 1:107.99", "A:457940901;C:403905608;G:399758720;T:463834300;N:6678359", 107, 107, null, null, 457940901, 403905608, 399758720, 463834300, 6678359, "SRX24539415", "SRS21284292", "SRA1865594", "Sirius University of Science and Technology|Genetics and Life Science", "Sirius University of Science and Technology", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "nebnext", "bulk", "unknown", "unknown", null, "Russia", "2024-05-13", "Adult", "Adult", "Brain", "Nervous System"], [32128, "SRR29012754", "SRX24539414", "SRS21284291", "SRP507393", "PRJNA1111146", "RNA seq of adult zebrafish brain following traumatic brain injury and/or ISRIB exposure", "PRJNA1111146", "Other", "Here analysed gene expression changes post Traumatic Brain Injury TBI and subsequent exposure to ISRIB in the brain of adult Danio rerio. 4 groups are available: control C  traumatic brain injury TBI; T  ISRIB injection I TBI and ISRIB injection TI.", null, null, null, null, "T1 TK22", null, "strain:Wild type|age:5 month|collection date:2023 08 20|geo loc name:Russia|sex:NA|tissue:brain|sample type:T1 TK22|treatment:TBI|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Here analysed gene expression changes post Traumatic Brain Injury TBI and subsequent exposure to ISRIB in the brain of adult Danio rerio. 4 groups are available: control C  traumatic brain injury TBI; T  ISRIB injection I TBI and ISRIB injection TI.", "TK22", "TK22", "RNA isolation was performed using the ExtractRNA reagent following the standard protocol for TriZol mediated extraction. The RNA quality was checked by capillary electrophoresis using the QIAxcel Advanced program. Total RNA was used for isolation of polyA fraction using NEBNext PolyA mRNA Magnetic Isolation Module according to the manufacturer's recommendations. RNA was next quantified on Quntus fluorometer and used for library preparation using NEBNext UltraTM Directional RNA Library PrepKit for Illumina with NEBNext Multiplex Oligos for Illumina according to the manufacturer's recommendations. The quality of libraries was confirmed by capillary electrophoresis using the QIAxcel Advanced program. Pair end sequencing was performed using Illumina HiSeq2500 with a read length of 110 bp.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA_oligo_dT", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP507393", null, null, "T1-TK23_S14_R1_001.fastq.gz T1-TK23_S14_R2_001.fastq.gz", "fastq fastq", 3331492486.0, 15432444.0, "T1 TK23 S14 R1 001.fastq.gz", "0:107.95 1:107.93", "A:868428457;C:786927841;G:785306451;T:875345180;N:15484557", 107, 107, null, null, 868428457, 786927841, 785306451, 875345180, 15484557, "SRX24539414", "SRS21284291", "SRA1865594", "Sirius University of Science and Technology|Genetics and Life Science", "Sirius University of Science and Technology", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "nebnext", "bulk", "unknown", "unknown", null, "Russia", "2024-05-13", "Adult", "Adult", "Brain", "Nervous System"], [32129, "SRR29012755", "SRX24539413", "SRS21284290", "SRP507393", "PRJNA1111146", "RNA seq of adult zebrafish brain following traumatic brain injury and/or ISRIB exposure", "PRJNA1111146", "Other", "Here analysed gene expression changes post Traumatic Brain Injury TBI and subsequent exposure to ISRIB in the brain of adult Danio rerio. 4 groups are available: control C  traumatic brain injury TBI; T  ISRIB injection I TBI and ISRIB injection TI.", null, null, null, null, "I6 TK16", null, "strain:Wild type|age:5 month|collection date:2023 08 20|geo loc name:Russia|sex:NA|tissue:brain|sample type:I6 TK16|treatment:ISRIB|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Here analysed gene expression changes post Traumatic Brain Injury TBI and subsequent exposure to ISRIB in the brain of adult Danio rerio. 4 groups are available: control C  traumatic brain injury TBI; T  ISRIB injection I TBI and ISRIB injection TI.", "TK16", "TK16", "RNA isolation was performed using the ExtractRNA reagent following the standard protocol for TriZol mediated extraction. The RNA quality was checked by capillary electrophoresis using the QIAxcel Advanced program. Total RNA was used for isolation of polyA fraction using NEBNext PolyA mRNA Magnetic Isolation Module according to the manufacturer's recommendations. RNA was next quantified on Quntus fluorometer and used for library preparation using NEBNext UltraTM Directional RNA Library PrepKit for Illumina with NEBNext Multiplex Oligos for Illumina according to the manufacturer's recommendations. The quality of libraries was confirmed by capillary electrophoresis using the QIAxcel Advanced program. Pair end sequencing was performed using Illumina HiSeq2500 with a read length of 110 bp.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA_oligo_dT", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP507393", null, null, "I6-TK16_S12_R1_001.fastq.gz I6-TK16_S12_R2_001.fastq.gz", "fastq fastq", 3714945017.0, 17207286.0, "I6 TK16 S12 R1 001.fastq.gz", "0:107.94 1:107.95", "A:1013736103;C:833395445;G:825731700;T:1025966376;N:16115393", 107, 107, null, null, 1013736103, 833395445, 825731700, 1025966376, 16115393, "SRX24539413", "SRS21284290", "SRA1865594", "Sirius University of Science and Technology|Genetics and Life Science", "Sirius University of Science and Technology", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "nebnext", "bulk", "unknown", "unknown", null, "Russia", "2024-05-13", "Adult", "Adult", "Brain", "Nervous System"], [32130, "SRR29012756", "SRX24539412", "SRS21284289", "SRP507393", "PRJNA1111146", "RNA seq of adult zebrafish brain following traumatic brain injury and/or ISRIB exposure", "PRJNA1111146", "Other", "Here analysed gene expression changes post Traumatic Brain Injury TBI and subsequent exposure to ISRIB in the brain of adult Danio rerio. 4 groups are available: control C  traumatic brain injury TBI; T  ISRIB injection I TBI and ISRIB injection TI.", null, null, null, null, "I5 TK15", null, "strain:Wild type|age:5 month|collection date:2023 08 20|geo loc name:Russia|sex:NA|tissue:brain|sample type:I5 TK15|treatment:ISRIB|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Here analysed gene expression changes post Traumatic Brain Injury TBI and subsequent exposure to ISRIB in the brain of adult Danio rerio. 4 groups are available: control C  traumatic brain injury TBI; T  ISRIB injection I TBI and ISRIB injection TI.", "TK15", "TK15", "RNA isolation was performed using the ExtractRNA reagent following the standard protocol for TriZol mediated extraction. The RNA quality was checked by capillary electrophoresis using the QIAxcel Advanced program. Total RNA was used for isolation of polyA fraction using NEBNext PolyA mRNA Magnetic Isolation Module according to the manufacturer's recommendations. RNA was next quantified on Quntus fluorometer and used for library preparation using NEBNext UltraTM Directional RNA Library PrepKit for Illumina with NEBNext Multiplex Oligos for Illumina according to the manufacturer's recommendations. The quality of libraries was confirmed by capillary electrophoresis using the QIAxcel Advanced program. Pair end sequencing was performed using Illumina HiSeq2500 with a read length of 110 bp.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA_oligo_dT", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP507393", null, null, "I5-TK15_S11_R1_001.fastq.gz I5-TK15_S11_R2_001.fastq.gz", "fastq fastq", 2794987343.0, 13014050.0, "I5 TK15 S11 R1 001.fastq.gz", "0:107.37 1:107.40", "A:774143942;C:614986331;G:609929626;T:782802382;N:13125062", 107, 107, null, null, 774143942, 614986331, 609929626, 782802382, 13125062, "SRX24539412", "SRS21284289", "SRA1865594", "Sirius University of Science and Technology|Genetics and Life Science", "Sirius University of Science and Technology", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "nebnext", "bulk", "unknown", "unknown", null, "Russia", "2024-05-13", "Adult", "Adult", "Brain", "Nervous System"], [32131, "SRR29012757", "SRX24539411", "SRS21284288", "SRP507393", "PRJNA1111146", "RNA seq of adult zebrafish brain following traumatic brain injury and/or ISRIB exposure", "PRJNA1111146", "Other", "Here analysed gene expression changes post Traumatic Brain Injury TBI and subsequent exposure to ISRIB in the brain of adult Danio rerio. 4 groups are available: control C  traumatic brain injury TBI; T  ISRIB injection I TBI and ISRIB injection TI.", null, null, null, null, "I4 TK14", null, "strain:Wild type|age:5 month|collection date:2023 08 20|geo loc name:Russia|sex:NA|tissue:brain|sample type:I4 TK14|treatment:ISRIB|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Here analysed gene expression changes post Traumatic Brain Injury TBI and subsequent exposure to ISRIB in the brain of adult Danio rerio. 4 groups are available: control C  traumatic brain injury TBI; T  ISRIB injection I TBI and ISRIB injection TI.", "TK14", "TK14", "RNA isolation was performed using the ExtractRNA reagent following the standard protocol for TriZol mediated extraction. The RNA quality was checked by capillary electrophoresis using the QIAxcel Advanced program. Total RNA was used for isolation of polyA fraction using NEBNext PolyA mRNA Magnetic Isolation Module according to the manufacturer's recommendations. RNA was next quantified on Quntus fluorometer and used for library preparation using NEBNext UltraTM Directional RNA Library PrepKit for Illumina with NEBNext Multiplex Oligos for Illumina according to the manufacturer's recommendations. The quality of libraries was confirmed by capillary electrophoresis using the QIAxcel Advanced program. Pair end sequencing was performed using Illumina HiSeq2500 with a read length of 110 bp.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA_oligo_dT", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP507393", null, null, "I4-TK14_S10_R1_001.fastq.gz I4-TK14_S10_R2_001.fastq.gz", "fastq fastq", 1922427285.0, 8937092.0, "I4 TK14 S10 R1 001.fastq.gz", "0:107.54 1:107.57", "A:528449546;C:426814810;G:423703279;T:534207858;N:9251792", 107, 107, null, null, 528449546, 426814810, 423703279, 534207858, 9251792, "SRX24539411", "SRS21284288", "SRA1865594", "Sirius University of Science and Technology|Genetics and Life Science", "Sirius University of Science and Technology", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "nebnext", "bulk", "unknown", "unknown", null, "Russia", "2024-05-13", "Adult", "Adult", "Brain", "Nervous System"], [32132, "SRR29012758", "SRX24539410", "SRS21284287", "SRP507393", "PRJNA1111146", "RNA seq of adult zebrafish brain following traumatic brain injury and/or ISRIB exposure", "PRJNA1111146", "Other", "Here analysed gene expression changes post Traumatic Brain Injury TBI and subsequent exposure to ISRIB in the brain of adult Danio rerio. 4 groups are available: control C  traumatic brain injury TBI; T  ISRIB injection I TBI and ISRIB injection TI.", null, null, null, null, "C2 TK32", null, "strain:Wild type|age:5 month|collection date:2023 08 20|geo loc name:Russia|sex:NA|tissue:brain|sample type:C2 TK32|treatment:Control|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Here analysed gene expression changes post Traumatic Brain Injury TBI and subsequent exposure to ISRIB in the brain of adult Danio rerio. 4 groups are available: control C  traumatic brain injury TBI; T  ISRIB injection I TBI and ISRIB injection TI.", "TK32", "TK32", "RNA isolation was performed using the ExtractRNA reagent following the standard protocol for TriZol mediated extraction. The RNA quality was checked by capillary electrophoresis using the QIAxcel Advanced program. Total RNA was used for isolation of polyA fraction using NEBNext PolyA mRNA Magnetic Isolation Module according to the manufacturer's recommendations. RNA was next quantified on Quntus fluorometer and used for library preparation using NEBNext UltraTM Directional RNA Library PrepKit for Illumina with NEBNext Multiplex Oligos for Illumina according to the manufacturer's recommendations. The quality of libraries was confirmed by capillary electrophoresis using the QIAxcel Advanced program. Pair end sequencing was performed using Illumina HiSeq2500 with a read length of 110 bp.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA_oligo_dT", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP507393", null, null, "C2-TK32_S21_R1_001.fastq.gz C2-TK32_S21_R2_001.fastq.gz", "fastq fastq", 3357957454.0, 15544937.0, "C2 TK32 S21 R1 001.fastq.gz", "0:108.00 1:108.01", "A:918342383;C:752111502;G:746458895;T:927695887;N:13348787", 108, 108, null, null, 918342383, 752111502, 746458895, 927695887, 13348787, "SRX24539410", "SRS21284287", "SRA1865594", "Sirius University of Science and Technology|Genetics and Life Science", "Sirius University of Science and Technology", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "nebnext", "bulk", "unknown", "unknown", null, "Russia", "2024-05-13", "Adult", "Adult", "Brain", "Nervous System"], [32133, "SRR29012759", "SRX24539409", "SRS21284286", "SRP507393", "PRJNA1111146", "RNA seq of adult zebrafish brain following traumatic brain injury and/or ISRIB exposure", "PRJNA1111146", "Other", "Here analysed gene expression changes post Traumatic Brain Injury TBI and subsequent exposure to ISRIB in the brain of adult Danio rerio. 4 groups are available: control C  traumatic brain injury TBI; T  ISRIB injection I TBI and ISRIB injection TI.", null, null, null, null, "C1 TK31", null, "strain:Wild type|age:5 month|collection date:2023 08 20|geo loc name:Russia|sex:NA|tissue:brain|sample type:C1 TK31|treatment:Control|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Here analysed gene expression changes post Traumatic Brain Injury TBI and subsequent exposure to ISRIB in the brain of adult Danio rerio. 4 groups are available: control C  traumatic brain injury TBI; T  ISRIB injection I TBI and ISRIB injection TI.", "TK31", "TK31", "RNA isolation was performed using the ExtractRNA reagent following the standard protocol for TriZol mediated extraction. The RNA quality was checked by capillary electrophoresis using the QIAxcel Advanced program. Total RNA was used for isolation of polyA fraction using NEBNext PolyA mRNA Magnetic Isolation Module according to the manufacturer's recommendations. RNA was next quantified on Quntus fluorometer and used for library preparation using NEBNext UltraTM Directional RNA Library PrepKit for Illumina with NEBNext Multiplex Oligos for Illumina according to the manufacturer's recommendations. The quality of libraries was confirmed by capillary electrophoresis using the QIAxcel Advanced program. Pair end sequencing was performed using Illumina HiSeq2500 with a read length of 110 bp.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA_oligo_dT", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP507393", null, null, "C1-TK31_S20_R1_001.fastq.gz C1-TK31_S20_R2_001.fastq.gz", "fastq fastq", 2442920945.0, 11344005.0, "C1 TK31 S20 R1 001.fastq.gz", "0:107.66 1:107.69", "A:668901972;C:544270967;G:539028537;T:676647377;N:14072092", 107, 107, null, null, 668901972, 544270967, 539028537, 676647377, 14072092, "SRX24539409", "SRS21284286", "SRA1865594", "Sirius University of Science and Technology|Genetics and Life Science", "Sirius University of Science and Technology", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "nebnext", "bulk", "unknown", "unknown", null, "Russia", "2024-05-13", "Adult", "Adult", "Brain", "Nervous System"], [33379, "SRR30184285", "SRX25650011", "SRS22293080", "SRP525334", "PRJNA1145997", "Regulation of optic nerve regeneration by srebf2", "PRJNA1145997", "Other", "Laser capture microdissection of the retinal ganglion cell layer followed by mRNA seq was used to measure differentially expressed genes in adult zebrafish at three days post optic nerve crush injury. Experimental groups include naive control  nonspecific morpholino control  morpholino mediated srebf2 gene knockdown  nonspecific morpholino control plus daily heat shock in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish  and morpholino mediated srebf2 gene knockdown in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish with daily heatshock. These data provide a resource to study the effects of srebf2 manipulation on transcriptomic responses to optic nerve injury in the adult zebrafish model.", null, null, null, "cMO 2", "wt cmo2 M", null, "strain:wild type|age:>3 month|collection date:2023 11 06|geo loc name:USA: Milwaukee  WI|sex:male|tissue:retinal ganglion cell layer|biomaterial provider:Matthew B. Veldman  Medical College of Wisconsin|treatment:cMO 2|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "LCM seq of Danio rerio:retinal ganglion cell layer", "L10", "L10", "LCM seq  NucleoSpin RNA XS purified  ultra low input RNAseq at Novogene", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA_oligo_dT", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP525334", null, null, "L10_S128_L005_R2_001.fastq.gz L10_S128_L005_R1_001.fastq.gz", "fastq fastq", 20328275944.0, 67312172.0, "L10 S128 L005 R1 001.fastq.gz", "0:151 1:151", "A:5643585269;C:4447562096;G:4432908344;T:5802780590;N:1439645", 151, 151, null, null, 5643585269, 4447562096, 4432908344, 5802780590, 1439645, "SRX25650011", "SRS22293080", "SRA1943640", "Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy", "Medical College of Wisconsin", 2, 0.89472, 0.89558, 0.14231, 0.14209, 0.76359, 0.76327, 0.57442, 0.57155, 151, 151, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-08-09", "Adult", "Adult", "Eye", "Sensory System"], [33380, "SRR30184286", "SRX25650010", "SRS22293078", "SRP525334", "PRJNA1145997", "Regulation of optic nerve regeneration by srebf2", "PRJNA1145997", "Other", "Laser capture microdissection of the retinal ganglion cell layer followed by mRNA seq was used to measure differentially expressed genes in adult zebrafish at three days post optic nerve crush injury. Experimental groups include naive control  nonspecific morpholino control  morpholino mediated srebf2 gene knockdown  nonspecific morpholino control plus daily heat shock in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish  and morpholino mediated srebf2 gene knockdown in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish with daily heatshock. These data provide a resource to study the effects of srebf2 manipulation on transcriptomic responses to optic nerve injury in the adult zebrafish model.", null, null, null, "cMO 1", "wt cmo1 M", null, "strain:wild type|age:>3 month|collection date:2023 11 06|geo loc name:USA: Milwaukee  WI|sex:male|tissue:retinal ganglion cell layer|biomaterial provider:Matthew B. Veldman  Medical College of Wisconsin|treatment:cMO 1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "LCM seq of Danio rerio:retinal ganglion cell layer", "L09", "L09", "LCM seq  NucleoSpin RNA XS purified  ultra low input RNAseq at Novogene", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA_oligo_dT", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP525334", null, null, "L09_S106_L004_R2_001.fastq.gz L09_S106_L004_R1_001.fastq.gz", "fastq fastq", 17931167252.0, 59374726.0, "L09 S106 L004 R1 001.fastq.gz", "0:151 1:151", "A:4724456408;C:4170780589;G:4192052775;T:4841803749;N:2073731", 151, 151, null, null, 4724456408, 4170780589, 4192052775, 4841803749, 2073731, "SRX25650010", "SRS22293078", "SRA1943640", "Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy", "Medical College of Wisconsin", 2, 0.92527, 0.92522, 0.05941, 0.05976, 0.76532, 0.76583, 0.55358, 0.55497, 151, 151, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-08-09", "Adult", "Adult", "Eye", "Sensory System"], [33381, "SRR30184287", "SRX25650009", "SRS22293079", "SRP525334", "PRJNA1145997", "Regulation of optic nerve regeneration by srebf2", "PRJNA1145997", "Other", "Laser capture microdissection of the retinal ganglion cell layer followed by mRNA seq was used to measure differentially expressed genes in adult zebrafish at three days post optic nerve crush injury. Experimental groups include naive control  nonspecific morpholino control  morpholino mediated srebf2 gene knockdown  nonspecific morpholino control plus daily heat shock in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish  and morpholino mediated srebf2 gene knockdown in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish with daily heatshock. These data provide a resource to study the effects of srebf2 manipulation on transcriptomic responses to optic nerve injury in the adult zebrafish model.", null, null, null, "3dpi 4", "wt R2 F", null, "strain:wild type|age:>3 month|collection date:2023 11 06|geo loc name:USA: Milwaukee  WI|sex:female|tissue:retinal ganglion cell layer|biomaterial provider:Matthew B. Veldman  Medical College of Wisconsin|treatment:3dpi 4|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "LCM seq of Danio rerio:retinal ganglion cell layer", "L08", "L08", "LCM seq  NucleoSpin RNA XS purified  ultra low input RNAseq at Novogene", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA_oligo_dT", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP525334", null, null, "L08_S127_L005_R2_001.fastq.gz L08_S127_L005_R1_001.fastq.gz", "fastq fastq", 22665495016.0, 75051308.0, "L08 S127 L005 R1 001.fastq.gz", "0:151 1:151", "A:6228385739;C:4990124759;G:4979460238;T:6465924079;N:1600201", 151, 151, null, null, 6228385739, 4990124759, 4979460238, 6465924079, 1600201, "SRX25650009", "SRS22293079", "SRA1943640", "Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy", "Medical College of Wisconsin", 2, 0.87648, 0.87921, 0.12966, 0.13019, 0.75627, 0.75666, 0.5783, 0.5754, 151, 151, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-08-09", "Adult", "Adult", "Eye", "Sensory System"], [33382, "SRR30184288", "SRX25650008", "SRS22293077", "SRP525334", "PRJNA1145997", "Regulation of optic nerve regeneration by srebf2", "PRJNA1145997", "Other", "Laser capture microdissection of the retinal ganglion cell layer followed by mRNA seq was used to measure differentially expressed genes in adult zebrafish at three days post optic nerve crush injury. Experimental groups include naive control  nonspecific morpholino control  morpholino mediated srebf2 gene knockdown  nonspecific morpholino control plus daily heat shock in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish  and morpholino mediated srebf2 gene knockdown in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish with daily heatshock. These data provide a resource to study the effects of srebf2 manipulation on transcriptomic responses to optic nerve injury in the adult zebrafish model.", null, null, null, "3dpi 3", "wt R1 F", null, "strain:wild type|age:>3 month|collection date:2023 11 06|geo loc name:USA: Milwaukee  WI|sex:female|tissue:retinal ganglion cell layer|biomaterial provider:Matthew B. Veldman  Medical College of Wisconsin|treatment:3dpi 3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "LCM seq of Danio rerio:retinal ganglion cell layer", "L07", "L07", "LCM seq  NucleoSpin RNA XS purified  ultra low input RNAseq at Novogene", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA_oligo_dT", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP525334", null, null, "L07_S126_L005_R2_001.fastq.gz L07_S126_L005_R1_001.fastq.gz", "fastq fastq", 22406825976.0, 74194788.0, "L07 S126 L005 R1 001.fastq.gz", "0:151 1:151", "A:6090398182;C:4986126981;G:5056253833;T:6272474188;N:1572792", 151, 151, null, null, 6090398182, 4986126981, 5056253833, 6272474188, 1572792, "SRX25650008", "SRS22293077", "SRA1943640", "Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy", "Medical College of Wisconsin", 2, 0.9153, 0.91559, 0.10005, 0.0998, 0.74679, 0.7469, 0.53845, 0.5395, 151, 151, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-08-09", "Adult", "Adult", "Eye", "Sensory System"], [33383, "SRR30184289", "SRX25650007", "SRS22293075", "SRP525334", "PRJNA1145997", "Regulation of optic nerve regeneration by srebf2", "PRJNA1145997", "Other", "Laser capture microdissection of the retinal ganglion cell layer followed by mRNA seq was used to measure differentially expressed genes in adult zebrafish at three days post optic nerve crush injury. Experimental groups include naive control  nonspecific morpholino control  morpholino mediated srebf2 gene knockdown  nonspecific morpholino control plus daily heat shock in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish  and morpholino mediated srebf2 gene knockdown in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish with daily heatshock. These data provide a resource to study the effects of srebf2 manipulation on transcriptomic responses to optic nerve injury in the adult zebrafish model.", null, null, null, "3dpi 2", "wt R2 M", null, "strain:wild type|age:>3 month|collection date:2023 11 06|geo loc name:USA: Milwaukee  WI|sex:male|tissue:retinal ganglion cell layer|biomaterial provider:Matthew B. Veldman  Medical College of Wisconsin|treatment:3dpi 2|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "LCM seq of Danio rerio:retinal ganglion cell layer", "L06", "L06", "LCM seq  NucleoSpin RNA XS purified  ultra low input RNAseq at Novogene", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA_oligo_dT", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP525334", null, null, "L06_S125_L005_R2_001.fastq.gz L06_S125_L005_R1_001.fastq.gz", "fastq fastq", 21918766796.0, 72578698.0, "L06 S125 L005 R1 001.fastq.gz", "0:151 1:151", "A:6030044320;C:4788979368;G:4790388904;T:6307807807;N:1546397", 151, 151, null, null, 6030044320, 4788979368, 4790388904, 6307807807, 1546397, "SRX25650007", "SRS22293075", "SRA1943640", "Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy", "Medical College of Wisconsin", 2, 0.87272, 0.87475, 0.15184, 0.15299, 0.76777, 0.76702, 0.58094, 0.57863, 151, 151, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-08-09", "Adult", "Adult", "Eye", "Sensory System"], [33384, "SRR30184290", "SRX25650006", "SRS22293076", "SRP525334", "PRJNA1145997", "Regulation of optic nerve regeneration by srebf2", "PRJNA1145997", "Other", "Laser capture microdissection of the retinal ganglion cell layer followed by mRNA seq was used to measure differentially expressed genes in adult zebrafish at three days post optic nerve crush injury. Experimental groups include naive control  nonspecific morpholino control  morpholino mediated srebf2 gene knockdown  nonspecific morpholino control plus daily heat shock in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish  and morpholino mediated srebf2 gene knockdown in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish with daily heatshock. These data provide a resource to study the effects of srebf2 manipulation on transcriptomic responses to optic nerve injury in the adult zebrafish model.", null, null, null, "3dpi 1", "wt R1 M", null, "strain:wild type|age:>3 month|collection date:2023 11 06|geo loc name:USA: Milwaukee  WI|sex:male|tissue:retinal ganglion cell layer|biomaterial provider:Matthew B. Veldman  Medical College of Wisconsin|treatment:3dpi 1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "LCM seq of Danio rerio:retinal ganglion cell layer", "L05", "L05", "LCM seq  NucleoSpin RNA XS purified  ultra low input RNAseq at Novogene", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA_oligo_dT", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP525334", null, null, "L05_S124_L005_R1_001.fastq.gz L05_S124_L005_R2_001.fastq.gz", "fastq fastq", 20254563180.0, 67068090.0, "L05 S124 L005 R1 001.fastq.gz", "0:151 1:151", "A:5433584775;C:4559357895;G:4610242789;T:5649933621;N:1444100", 151, 151, null, null, 5433584775, 4559357895, 4610242789, 5649933621, 1444100, "SRX25650006", "SRS22293076", "SRA1943640", "Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy", "Medical College of Wisconsin", 2, 0.90628, 0.90764, 0.08395, 0.08422, 0.7712, 0.77086, 0.5176, 0.51705, 151, 151, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-08-09", "Adult", "Adult", "Eye", "Sensory System"], [33385, "SRR30184291", "SRX25650005", "SRS22293074", "SRP525334", "PRJNA1145997", "Regulation of optic nerve regeneration by srebf2", "PRJNA1145997", "Other", "Laser capture microdissection of the retinal ganglion cell layer followed by mRNA seq was used to measure differentially expressed genes in adult zebrafish at three days post optic nerve crush injury. Experimental groups include naive control  nonspecific morpholino control  morpholino mediated srebf2 gene knockdown  nonspecific morpholino control plus daily heat shock in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish  and morpholino mediated srebf2 gene knockdown in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish with daily heatshock. These data provide a resource to study the effects of srebf2 manipulation on transcriptomic responses to optic nerve injury in the adult zebrafish model.", null, null, null, "uninjured 4", "wt L2 F", null, "strain:wild type|age:>3 month|collection date:2023 11 06|geo loc name:USA: Milwaukee  WI|sex:female|tissue:retinal ganglion cell layer|biomaterial provider:Matthew B. Veldman  Medical College of Wisconsin|treatment:uninjured 4|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "LCM seq of Danio rerio:retinal ganglion cell layer", "L04", "L04", "LCM seq  NucleoSpin RNA XS purified  ultra low input RNAseq at Novogene", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA_oligo_dT", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP525334", null, null, "L04_S142_L006_R2_001.fastq.gz L04_S142_L006_R1_001.fastq.gz", "fastq fastq", 29812977034.0, 98718467.0, "L04 S142 L006 R1 001.fastq.gz", "0:151 1:151", "A:8014887204;C:6674942655;G:6635556218;T:8483149485;N:4441472", 151, 151, null, null, 8014887204, 6674942655, 6635556218, 8483149485, 4441472, "SRX25650005", "SRS22293074", "SRA1943640", "Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy", "Medical College of Wisconsin", 2, 0.84638, 0.84891, 0.16019, 0.1609, 0.77737, 0.77733, 0.62585, 0.63219, 151, 151, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-08-09", "Adult", "Adult", "Eye", "Sensory System"], [33386, "SRR30184292", "SRX25650004", "SRS22293073", "SRP525334", "PRJNA1145997", "Regulation of optic nerve regeneration by srebf2", "PRJNA1145997", "Other", "Laser capture microdissection of the retinal ganglion cell layer followed by mRNA seq was used to measure differentially expressed genes in adult zebrafish at three days post optic nerve crush injury. Experimental groups include naive control  nonspecific morpholino control  morpholino mediated srebf2 gene knockdown  nonspecific morpholino control plus daily heat shock in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish  and morpholino mediated srebf2 gene knockdown in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish with daily heatshock. These data provide a resource to study the effects of srebf2 manipulation on transcriptomic responses to optic nerve injury in the adult zebrafish model.", null, null, null, "heatshock srebf2MO 4", "hs smo2 F", null, "strain:Tghsp70l:nSrebf2 2AmCherry  cmcl2:EGFP|age:>3 month|collection date:2023 11 06|geo loc name:USA: Milwaukee  WI|sex:female|tissue:retinal ganglion cell layer|biomaterial provider:Matthew B. Veldman  Medical College of Wisconsin|treatment:heatshock srebf2MO 4|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "LCM seq of Danio rerio:retinal ganglion cell layer", "L28", "L28", "LCM seq  NucleoSpin RNA XS purified  ultra low input RNAseq at Novogene", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA_oligo_dT", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP525334", null, null, "L28_S116_L004_R2_001.fastq.gz L28_S116_L004_R1_001.fastq.gz", "fastq fastq", 17026943012.0, 56380606.0, "L28 S116 L004 R1 001.fastq.gz", "0:151 1:151", "A:4669306614;C:3826935006;G:3777947200;T:4750777220;N:1976972", 151, 151, null, null, 4669306614, 3826935006, 3777947200, 4750777220, 1976972, "SRX25650004", "SRS22293073", "SRA1943640", "Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy", "Medical College of Wisconsin", 2, 0.93284, 0.93235, 0.07802, 0.07812, 0.75568, 0.75597, 0.55431, 0.54921, 151, 151, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-08-09", "Adult", "Adult", "Eye", "Sensory System"], [33387, "SRR30184293", "SRX25650003", "SRS22293072", "SRP525334", "PRJNA1145997", "Regulation of optic nerve regeneration by srebf2", "PRJNA1145997", "Other", "Laser capture microdissection of the retinal ganglion cell layer followed by mRNA seq was used to measure differentially expressed genes in adult zebrafish at three days post optic nerve crush injury. Experimental groups include naive control  nonspecific morpholino control  morpholino mediated srebf2 gene knockdown  nonspecific morpholino control plus daily heat shock in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish  and morpholino mediated srebf2 gene knockdown in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish with daily heatshock. These data provide a resource to study the effects of srebf2 manipulation on transcriptomic responses to optic nerve injury in the adult zebrafish model.", null, null, null, "heatshock srebf2MO 3", "hs smo1 F", null, "strain:Tghsp70l:nSrebf2 2AmCherry  cmcl2:EGFP|age:>3 month|collection date:2023 11 06|geo loc name:USA: Milwaukee  WI|sex:female|tissue:retinal ganglion cell layer|biomaterial provider:Matthew B. Veldman  Medical College of Wisconsin|treatment:heatshock srebf2MO 3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "LCM seq of Danio rerio:retinal ganglion cell layer", "L27", "L27", "LCM seq  NucleoSpin RNA XS purified  ultra low input RNAseq at Novogene", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA_oligo_dT", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP525334", null, null, "L27_S115_L004_R2_001.fastq.gz L27_S115_L004_R1_001.fastq.gz", "fastq fastq", 22610315690.0, 74868595.0, "L27 S115 L004 R1 001.fastq.gz", "0:151 1:151", "A:6205491974;C:5075129196;G:4965212019;T:6361870398;N:2612103", 151, 151, null, null, 6205491974, 5075129196, 4965212019, 6361870398, 2612103, "SRX25650003", "SRS22293072", "SRA1943640", "Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy", "Medical College of Wisconsin", 2, 0.78769, 0.78807, 0.09282, 0.09299, 0.76619, 0.7651, 0.54884, 0.5509, 151, 151, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-08-09", "Adult", "Adult", "Eye", "Sensory System"], [33388, "SRR30184294", "SRX25650002", "SRS22293071", "SRP525334", "PRJNA1145997", "Regulation of optic nerve regeneration by srebf2", "PRJNA1145997", "Other", "Laser capture microdissection of the retinal ganglion cell layer followed by mRNA seq was used to measure differentially expressed genes in adult zebrafish at three days post optic nerve crush injury. Experimental groups include naive control  nonspecific morpholino control  morpholino mediated srebf2 gene knockdown  nonspecific morpholino control plus daily heat shock in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish  and morpholino mediated srebf2 gene knockdown in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish with daily heatshock. These data provide a resource to study the effects of srebf2 manipulation on transcriptomic responses to optic nerve injury in the adult zebrafish model.", null, null, null, "heatshock srebf2MO 2", "hs smo2 M", null, "strain:Tghsp70l:nSrebf2 2AmCherry  cmcl2:EGFP|age:>3 month|collection date:2023 11 06|geo loc name:USA: Milwaukee  WI|sex:male|tissue:retinal ganglion cell layer|biomaterial provider:Matthew B. Veldman  Medical College of Wisconsin|treatment:heatshock srebf2MO 2|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "LCM seq of Danio rerio:retinal ganglion cell layer", "L26", "L26", "LCM seq  NucleoSpin RNA XS purified  ultra low input RNAseq at Novogene", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA_oligo_dT", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP525334", null, null, "L26_S144_L006_R2_001.fastq.gz L26_S144_L006_R1_001.fastq.gz", "fastq fastq", 17129465368.0, 56720084.0, "L26 S144 L006 R1 001.fastq.gz", "0:151 1:151", "A:4750698191;C:3734802838;G:3895289691;T:4746170435;N:2504213", 151, 151, null, null, 4750698191, 3734802838, 3895289691, 4746170435, 2504213, "SRX25650002", "SRS22293071", "SRA1943640", "Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy", "Medical College of Wisconsin", 2, 0.93654, 0.92792, 0.07609, 0.07622, 0.75061, 0.75357, 0.52583, 0.53272, 151, 151, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-08-09", "Adult", "Adult", "Eye", "Sensory System"], [33389, "SRR30184295", "SRX25650001", "SRS22293070", "SRP525334", "PRJNA1145997", "Regulation of optic nerve regeneration by srebf2", "PRJNA1145997", "Other", "Laser capture microdissection of the retinal ganglion cell layer followed by mRNA seq was used to measure differentially expressed genes in adult zebrafish at three days post optic nerve crush injury. Experimental groups include naive control  nonspecific morpholino control  morpholino mediated srebf2 gene knockdown  nonspecific morpholino control plus daily heat shock in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish  and morpholino mediated srebf2 gene knockdown in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish with daily heatshock. These data provide a resource to study the effects of srebf2 manipulation on transcriptomic responses to optic nerve injury in the adult zebrafish model.", null, null, null, "heatshock srebf2MO 1", "hs smo1 M", null, "strain:Tghsp70l:nSrebf2 2AmCherry  cmcl2:EGFP|age:>3 month|collection date:2023 11 06|geo loc name:USA: Milwaukee  WI|sex:male|tissue:retinal ganglion cell layer|biomaterial provider:Matthew B. Veldman  Medical College of Wisconsin|treatment:heatshock srebf2MO 1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "LCM seq of Danio rerio:retinal ganglion cell layer", "L25", "L25", "LCM seq  NucleoSpin RNA XS purified  ultra low input RNAseq at Novogene", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA_oligo_dT", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP525334", null, null, "L25_S114_L004_R2_001.fastq.gz L25_S114_L004_R1_001.fastq.gz", "fastq fastq", 18411870316.0, 60966458.0, "L25 S114 L004 R1 001.fastq.gz", "0:151 1:151", "A:5037571347;C:4144572498;G:4103153869;T:5124440844;N:2131758", 151, 151, null, null, 5037571347, 4144572498, 4103153869, 5124440844, 2131758, "SRX25650001", "SRS22293070", "SRA1943640", "Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy", "Medical College of Wisconsin", 2, 0.92822, 0.92792, 0.0886, 0.08918, 0.75974, 0.75978, 0.55638, 0.55618, 151, 151, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-08-09", "Adult", "Adult", "Eye", "Sensory System"], [33390, "SRR30184296", "SRX25650000", "SRS22293066", "SRP525334", "PRJNA1145997", "Regulation of optic nerve regeneration by srebf2", "PRJNA1145997", "Other", "Laser capture microdissection of the retinal ganglion cell layer followed by mRNA seq was used to measure differentially expressed genes in adult zebrafish at three days post optic nerve crush injury. Experimental groups include naive control  nonspecific morpholino control  morpholino mediated srebf2 gene knockdown  nonspecific morpholino control plus daily heat shock in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish  and morpholino mediated srebf2 gene knockdown in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish with daily heatshock. These data provide a resource to study the effects of srebf2 manipulation on transcriptomic responses to optic nerve injury in the adult zebrafish model.", null, null, null, "uninjured 3", "wt L1 F", null, "strain:wild type|age:>3 month|collection date:2023 11 06|geo loc name:USA: Milwaukee  WI|sex:female|tissue:retinal ganglion cell layer|biomaterial provider:Matthew B. Veldman  Medical College of Wisconsin|treatment:uninjured 3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "LCM seq of Danio rerio:retinal ganglion cell layer", "L03", "L03", "LCM seq  NucleoSpin RNA XS purified  ultra low input RNAseq at Novogene", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA_oligo_dT", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP525334", null, null, "L03_S105_L004_R1_001.fastq.gz L03_S105_L004_R2_001.fastq.gz", "fastq fastq", 19718182792.0, 65291996.0, "L03 S105 L004 R1 001.fastq.gz", "0:151 1:151", "A:5247234351;C:4504410887;G:4526181297;T:5438074144;N:2282113", 151, 151, null, null, 5247234351, 4504410887, 4526181297, 5438074144, 2282113, "SRX25650000", "SRS22293066", "SRA1943640", "Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy", "Medical College of Wisconsin", 2, 0.90475, 0.90699, 0.11305, 0.11331, 0.75014, 0.74929, 0.52801, 0.54316, 151, 151, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-08-09", "Adult", "Adult", "Eye", "Sensory System"], [33391, "SRR30184297", "SRX25649999", "SRS22293069", "SRP525334", "PRJNA1145997", "Regulation of optic nerve regeneration by srebf2", "PRJNA1145997", "Other", "Laser capture microdissection of the retinal ganglion cell layer followed by mRNA seq was used to measure differentially expressed genes in adult zebrafish at three days post optic nerve crush injury. Experimental groups include naive control  nonspecific morpholino control  morpholino mediated srebf2 gene knockdown  nonspecific morpholino control plus daily heat shock in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish  and morpholino mediated srebf2 gene knockdown in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish with daily heatshock. These data provide a resource to study the effects of srebf2 manipulation on transcriptomic responses to optic nerve injury in the adult zebrafish model.", null, null, null, "heatshock cMO 4", "hs cmo2 F", null, "strain:Tghsp70l:nSrebf2 2AmCherry  cmcl2:EGFP|age:>3 month|collection date:2023 11 06|geo loc name:USA: Milwaukee  WI|sex:female|tissue:retinal ganglion cell layer|biomaterial provider:Matthew B. Veldman  Medical College of Wisconsin|treatment:heatshock cMO 4|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "LCM seq of Danio rerio:retinal ganglion cell layer", "L24", "L24", "LCM seq  NucleoSpin RNA XS purified  ultra low input RNAseq at Novogene", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA_oligo_dT", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP525334", null, null, "L24_S134_L005_R2_001.fastq.gz L24_S134_L005_R1_001.fastq.gz", "fastq fastq", 19377661484.0, 64164442.0, "L24 S134 L005 R1 001.fastq.gz", "0:151 1:151", "A:5371834087;C:4284931090;G:4227428342;T:5492109103;N:1358862", 151, 151, null, null, 5371834087, 4284931090, 4227428342, 5492109103, 1358862, "SRX25649999", "SRS22293069", "SRA1943640", "Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy", "Medical College of Wisconsin", 2, 0.9104, 0.90922, 0.11083, 0.11062, 0.75325, 0.7543, 0.54602, 0.54303, 151, 151, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-08-09", "Adult", "Adult", "Eye", "Sensory System"], [33392, "SRR30184298", "SRX25649998", "SRS22293067", "SRP525334", "PRJNA1145997", "Regulation of optic nerve regeneration by srebf2", "PRJNA1145997", "Other", "Laser capture microdissection of the retinal ganglion cell layer followed by mRNA seq was used to measure differentially expressed genes in adult zebrafish at three days post optic nerve crush injury. Experimental groups include naive control  nonspecific morpholino control  morpholino mediated srebf2 gene knockdown  nonspecific morpholino control plus daily heat shock in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish  and morpholino mediated srebf2 gene knockdown in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish with daily heatshock. These data provide a resource to study the effects of srebf2 manipulation on transcriptomic responses to optic nerve injury in the adult zebrafish model.", null, null, null, "heatshock cMO 3", "hs cmo1 F", null, "strain:Tghsp70l:nSrebf2 2AmCherry  cmcl2:EGFP|age:>3 month|collection date:2023 11 06|geo loc name:USA: Milwaukee  WI|sex:female|tissue:retinal ganglion cell layer|biomaterial provider:Matthew B. Veldman  Medical College of Wisconsin|treatment:heatshock cMO 3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "LCM seq of Danio rerio:retinal ganglion cell layer", "L23", "L23", "LCM seq  NucleoSpin RNA XS purified  ultra low input RNAseq at Novogene", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA_oligo_dT", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP525334", null, null, "L23_S143_L006_R2_001.fastq.gz L23_S143_L006_R1_001.fastq.gz", "fastq fastq", 28079439956.0, 92978278.0, "L23 S143 L006 R1 001.fastq.gz", "0:151 1:151", "A:7811720040;C:6084170964;G:6064272363;T:8115079585;N:4197004", 151, 151, null, null, 7811720040, 6084170964, 6064272363, 8115079585, 4197004, "SRX25649998", "SRS22293067", "SRA1943640", "Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy", "Medical College of Wisconsin", 2, 0.86593, 0.86525, 0.14584, 0.14587, 0.77498, 0.77433, 0.59534, 0.59433, 151, 151, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-08-09", "Adult", "Adult", "Eye", "Sensory System"], [33393, "SRR30184299", "SRX25649997", "SRS22293068", "SRP525334", "PRJNA1145997", "Regulation of optic nerve regeneration by srebf2", "PRJNA1145997", "Other", "Laser capture microdissection of the retinal ganglion cell layer followed by mRNA seq was used to measure differentially expressed genes in adult zebrafish at three days post optic nerve crush injury. Experimental groups include naive control  nonspecific morpholino control  morpholino mediated srebf2 gene knockdown  nonspecific morpholino control plus daily heat shock in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish  and morpholino mediated srebf2 gene knockdown in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish with daily heatshock. These data provide a resource to study the effects of srebf2 manipulation on transcriptomic responses to optic nerve injury in the adult zebrafish model.", null, null, null, "heatshock cMO 2", "hs cmo2 M", null, "strain:Tghsp70l:nSrebf2 2AmCherry  cmcl2:EGFP|age:>3 month|collection date:2023 11 06|geo loc name:USA: Milwaukee  WI|sex:male|tissue:retinal ganglion cell layer|biomaterial provider:Matthew B. Veldman  Medical College of Wisconsin|treatment:heatshock cMO 2|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "LCM seq of Danio rerio:retinal ganglion cell layer", "L22", "L22", "LCM seq  NucleoSpin RNA XS purified  ultra low input RNAseq at Novogene", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA_oligo_dT", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP525334", null, null, "L22_S133_L005_R2_001.fastq.gz L22_S133_L005_R1_001.fastq.gz", "fastq fastq", 20494382588.0, 67862194.0, "L22 S133 L005 R1 001.fastq.gz", "0:151 1:151", "A:5610165229;C:4583590395;G:4590759271;T:5708435247;N:1432446", 151, 151, null, null, 5610165229, 4583590395, 4590759271, 5708435247, 1432446, "SRX25649997", "SRS22293068", "SRA1943640", "Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy", "Medical College of Wisconsin", 2, 0.92306, 0.92279, 0.0879, 0.08765, 0.7671, 0.76579, 0.56402, 0.56401, 151, 151, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-08-09", "Adult", "Adult", "Eye", "Sensory System"], [33394, "SRR30184300", "SRX25649996", "SRS22293064", "SRP525334", "PRJNA1145997", "Regulation of optic nerve regeneration by srebf2", "PRJNA1145997", "Other", "Laser capture microdissection of the retinal ganglion cell layer followed by mRNA seq was used to measure differentially expressed genes in adult zebrafish at three days post optic nerve crush injury. Experimental groups include naive control  nonspecific morpholino control  morpholino mediated srebf2 gene knockdown  nonspecific morpholino control plus daily heat shock in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish  and morpholino mediated srebf2 gene knockdown in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish with daily heatshock. These data provide a resource to study the effects of srebf2 manipulation on transcriptomic responses to optic nerve injury in the adult zebrafish model.", null, null, null, "heatshock cMO 1", "hs cmo1 M", null, "strain:Tghsp70l:nSrebf2 2AmCherry  cmcl2:EGFP|age:>3 month|collection date:2023 11 06|geo loc name:USA: Milwaukee  WI|sex:male|tissue:retinal ganglion cell layer|biomaterial provider:Matthew B. Veldman  Medical College of Wisconsin|treatment:heatshock cMO 1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "LCM seq of Danio rerio:retinal ganglion cell layer", "L21", "L21", "LCM seq  NucleoSpin RNA XS purified  ultra low input RNAseq at Novogene", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA_oligo_dT", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP525334", null, null, "L21_S113_L004_R1_001.fastq.gz L21_S113_L004_R2_001.fastq.gz", "fastq fastq", 22282038670.0, 73781585.0, "L21 S113 L004 R1 001.fastq.gz", "0:151 1:151", "A:5984432885;C:5103219794;G:5123288852;T:6068507910;N:2589229", 151, 151, null, null, 5984432885, 5103219794, 5123288852, 6068507910, 2589229, "SRX25649996", "SRS22293064", "SRA1943640", "Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy", "Medical College of Wisconsin", 2, 0.93523, 0.93442, 0.06557, 0.06558, 0.76463, 0.76532, 0.54365, 0.53711, 151, 151, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-08-09", "Adult", "Adult", "Eye", "Sensory System"], [33395, "SRR30184301", "SRX25649995", "SRS22293061", "SRP525334", "PRJNA1145997", "Regulation of optic nerve regeneration by srebf2", "PRJNA1145997", "Other", "Laser capture microdissection of the retinal ganglion cell layer followed by mRNA seq was used to measure differentially expressed genes in adult zebrafish at three days post optic nerve crush injury. Experimental groups include naive control  nonspecific morpholino control  morpholino mediated srebf2 gene knockdown  nonspecific morpholino control plus daily heat shock in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish  and morpholino mediated srebf2 gene knockdown in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish with daily heatshock. These data provide a resource to study the effects of srebf2 manipulation on transcriptomic responses to optic nerve injury in the adult zebrafish model.", null, null, null, "srebf2MO 4", "wt smo2 F", null, "strain:wild type|age:>3 month|collection date:2023 11 06|geo loc name:USA: Milwaukee  WI|sex:female|tissue:retinal ganglion cell layer|biomaterial provider:Matthew B. Veldman  Medical College of Wisconsin|treatment:srebf2MO 4|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "LCM seq of Danio rerio:retinal ganglion cell layer", "L16", "L16", "LCM seq  NucleoSpin RNA XS purified  ultra low input RNAseq at Novogene", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA_oligo_dT", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP525334", null, null, "L16_S108_L004_R1_001.fastq.gz L16_S108_L004_R2_001.fastq.gz", "fastq fastq", 25364244630.0, 83987565.0, "L16 S108 L004 R1 001.fastq.gz", "0:151 1:151", "A:6783811492;C:5825885841;G:5791188806;T:6960421955;N:2936536", 151, 151, null, null, 6783811492, 5825885841, 5791188806, 6960421955, 2936536, "SRX25649995", "SRS22293061", "SRA1943640", "Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy", "Medical College of Wisconsin", 2, 0.93174, 0.93131, 0.06871, 0.06898, 0.75461, 0.75386, 0.54103, 0.54073, 151, 151, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-08-09", "Adult", "Adult", "Eye", "Sensory System"], [33396, "SRR30184302", "SRX25649994", "SRS22293065", "SRP525334", "PRJNA1145997", "Regulation of optic nerve regeneration by srebf2", "PRJNA1145997", "Other", "Laser capture microdissection of the retinal ganglion cell layer followed by mRNA seq was used to measure differentially expressed genes in adult zebrafish at three days post optic nerve crush injury. Experimental groups include naive control  nonspecific morpholino control  morpholino mediated srebf2 gene knockdown  nonspecific morpholino control plus daily heat shock in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish  and morpholino mediated srebf2 gene knockdown in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish with daily heatshock. These data provide a resource to study the effects of srebf2 manipulation on transcriptomic responses to optic nerve injury in the adult zebrafish model.", null, null, null, "srebf2MO 3", "wt smo1 F", null, "strain:wild type|age:>3 month|collection date:2023 11 06|geo loc name:USA: Milwaukee  WI|sex:female|tissue:retinal ganglion cell layer|biomaterial provider:Matthew B. Veldman  Medical College of Wisconsin|treatment:srebf2MO 3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "LCM seq of Danio rerio:retinal ganglion cell layer", "L15", "L15", "LCM seq  NucleoSpin RNA XS purified  ultra low input RNAseq at Novogene", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA_oligo_dT", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP525334", null, null, "L15_S132_L005_R2_001.fastq.gz L15_S132_L005_R1_001.fastq.gz", "fastq fastq", 24643120876.0, 81599738.0, "L15 S132 L005 R1 001.fastq.gz", "0:151 1:151", "A:6791560596;C:5441282614;G:5412453327;T:6996084050;N:1740289", 151, 151, null, null, 6791560596, 5441282614, 5412453327, 6996084050, 1740289, "SRX25649994", "SRS22293065", "SRA1943640", "Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy", "Medical College of Wisconsin", 2, 0.90806, 0.90874, 0.11049, 0.11033, 0.75885, 0.75741, 0.54345, 0.55215, 151, 151, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-08-09", "Adult", "Adult", "Eye", "Sensory System"], [33397, "SRR30184303", "SRX25649993", "SRS22293062", "SRP525334", "PRJNA1145997", "Regulation of optic nerve regeneration by srebf2", "PRJNA1145997", "Other", "Laser capture microdissection of the retinal ganglion cell layer followed by mRNA seq was used to measure differentially expressed genes in adult zebrafish at three days post optic nerve crush injury. Experimental groups include naive control  nonspecific morpholino control  morpholino mediated srebf2 gene knockdown  nonspecific morpholino control plus daily heat shock in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish  and morpholino mediated srebf2 gene knockdown in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish with daily heatshock. These data provide a resource to study the effects of srebf2 manipulation on transcriptomic responses to optic nerve injury in the adult zebrafish model.", null, null, null, "srebf2MO 2", "wt smo2 M", null, "strain:wild type|age:>3 month|collection date:2023 11 06|geo loc name:USA: Milwaukee  WI|sex:male|tissue:retinal ganglion cell layer|biomaterial provider:Matthew B. Veldman  Medical College of Wisconsin|treatment:srebf2MO 2|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "LCM seq of Danio rerio:retinal ganglion cell layer", "L14", "L14", "LCM seq  NucleoSpin RNA XS purified  ultra low input RNAseq at Novogene", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA_oligo_dT", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP525334", null, null, "L14_S131_L005_R2_001.fastq.gz L14_S131_L005_R1_001.fastq.gz", "fastq fastq", 20984328060.0, 69484530.0, "L14 S131 L005 R1 001.fastq.gz", "0:151 1:151", "A:5728503785;C:4710613293;G:4687638412;T:5856111147;N:1461423", 151, 151, null, null, 5728503785, 4710613293, 4687638412, 5856111147, 1461423, "SRX25649993", "SRS22293062", "SRA1943640", "Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy", "Medical College of Wisconsin", 2, 0.92516, 0.92471, 0.08416, 0.08435, 0.76568, 0.76585, 0.56286, 0.56146, 151, 151, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-08-09", "Adult", "Adult", "Eye", "Sensory System"], [33398, "SRR30184304", "SRX25649992", "SRS22293059", "SRP525334", "PRJNA1145997", "Regulation of optic nerve regeneration by srebf2", "PRJNA1145997", "Other", "Laser capture microdissection of the retinal ganglion cell layer followed by mRNA seq was used to measure differentially expressed genes in adult zebrafish at three days post optic nerve crush injury. Experimental groups include naive control  nonspecific morpholino control  morpholino mediated srebf2 gene knockdown  nonspecific morpholino control plus daily heat shock in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish  and morpholino mediated srebf2 gene knockdown in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish with daily heatshock. These data provide a resource to study the effects of srebf2 manipulation on transcriptomic responses to optic nerve injury in the adult zebrafish model.", null, null, null, "srebf2MO 1", "wt smo1 M", null, "strain:wild type|age:>3 month|collection date:2023 11 06|geo loc name:USA: Milwaukee  WI|sex:male|tissue:retinal ganglion cell layer|biomaterial provider:Matthew B. Veldman  Medical College of Wisconsin|treatment:srebf2MO 1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "LCM seq of Danio rerio:retinal ganglion cell layer", "L13", "L13", "LCM seq  NucleoSpin RNA XS purified  ultra low input RNAseq at Novogene", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA_oligo_dT", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP525334", null, null, "L13_S130_L005_R1_001.fastq.gz L13_S130_L005_R2_001.fastq.gz", "fastq fastq", 17976563590.0, 59525045.0, "L13 S130 L005 R1 001.fastq.gz", "0:151 1:151", "A:4931490430;C:3978811459;G:3956968246;T:5108014615;N:1278840", 151, 151, null, null, 4931490430, 3978811459, 3956968246, 5108014615, 1278840, "SRX25649992", "SRS22293059", "SRA1943640", "Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy", "Medical College of Wisconsin", 2, 0.8948, 0.89491, 0.13113, 0.13061, 0.76254, 0.76246, 0.57407, 0.57069, 151, 151, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-08-09", "Adult", "Adult", "Eye", "Sensory System"], [33399, "SRR30184305", "SRX25649991", "SRS22293060", "SRP525334", "PRJNA1145997", "Regulation of optic nerve regeneration by srebf2", "PRJNA1145997", "Other", "Laser capture microdissection of the retinal ganglion cell layer followed by mRNA seq was used to measure differentially expressed genes in adult zebrafish at three days post optic nerve crush injury. Experimental groups include naive control  nonspecific morpholino control  morpholino mediated srebf2 gene knockdown  nonspecific morpholino control plus daily heat shock in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish  and morpholino mediated srebf2 gene knockdown in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish with daily heatshock. These data provide a resource to study the effects of srebf2 manipulation on transcriptomic responses to optic nerve injury in the adult zebrafish model.", null, null, null, "cMO 4", "wt cmo2 F", null, "strain:wild type|age:>3 month|collection date:2023 11 06|geo loc name:USA: Milwaukee  WI|sex:female|tissue:retinal ganglion cell layer|biomaterial provider:Matthew B. Veldman  Medical College of Wisconsin|treatment:cMO 4|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "LCM seq of Danio rerio:retinal ganglion cell layer", "L12", "L12", "LCM seq  NucleoSpin RNA XS purified  ultra low input RNAseq at Novogene", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA_oligo_dT", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP525334", null, null, "L12_S129_L005_R2_001.fastq.gz L12_S129_L005_R1_001.fastq.gz", "fastq fastq", 19905894214.0, 65913557.0, "L12 S129 L005 R1 001.fastq.gz", "0:151 1:151", "A:5382577098;C:4496451576;G:4493042842;T:5532424959;N:1397739", 151, 151, null, null, 5382577098, 4496451576, 4493042842, 5532424959, 1397739, "SRX25649991", "SRS22293060", "SRA1943640", "Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy", "Medical College of Wisconsin", 2, 0.9238, 0.92347, 0.08792, 0.08777, 0.75933, 0.75921, 0.56478, 0.567, 151, 151, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-08-09", "Adult", "Adult", "Eye", "Sensory System"], [33400, "SRR30184306", "SRX25649990", "SRS22293063", "SRP525334", "PRJNA1145997", "Regulation of optic nerve regeneration by srebf2", "PRJNA1145997", "Other", "Laser capture microdissection of the retinal ganglion cell layer followed by mRNA seq was used to measure differentially expressed genes in adult zebrafish at three days post optic nerve crush injury. Experimental groups include naive control  nonspecific morpholino control  morpholino mediated srebf2 gene knockdown  nonspecific morpholino control plus daily heat shock in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish  and morpholino mediated srebf2 gene knockdown in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish with daily heatshock. These data provide a resource to study the effects of srebf2 manipulation on transcriptomic responses to optic nerve injury in the adult zebrafish model.", null, null, null, "cMO 3", "wt cmo1 F", null, "strain:wild type|age:>3 month|collection date:2023 11 06|geo loc name:USA: Milwaukee  WI|sex:female|tissue:retinal ganglion cell layer|biomaterial provider:Matthew B. Veldman  Medical College of Wisconsin|treatment:cMO 3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "LCM seq of Danio rerio:retinal ganglion cell layer", "L11", "L11", "LCM seq  NucleoSpin RNA XS purified  ultra low input RNAseq at Novogene", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA_oligo_dT", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP525334", null, null, "L11_S107_L004_R1_001.fastq.gz L11_S107_L004_R2_001.fastq.gz", "fastq fastq", 17297093488.0, 57275144.0, "L11 S107 L004 R1 001.fastq.gz", "0:151 1:151", "A:4727002988;C:3860128821;G:3837802284;T:4870157333;N:2002062", 151, 151, null, null, 4727002988, 3860128821, 3837802284, 4870157333, 2002062, "SRX25649990", "SRS22293063", "SRA1943640", "Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy", "Medical College of Wisconsin", 2, 0.86746, 0.86798, 0.10604, 0.1069, 0.76396, 0.7639, 0.56148, 0.55457, 151, 151, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-08-09", "Adult", "Adult", "Eye", "Sensory System"], [33401, "SRR30184307", "SRX25649989", "SRS22293058", "SRP525334", "PRJNA1145997", "Regulation of optic nerve regeneration by srebf2", "PRJNA1145997", "Other", "Laser capture microdissection of the retinal ganglion cell layer followed by mRNA seq was used to measure differentially expressed genes in adult zebrafish at three days post optic nerve crush injury. Experimental groups include naive control  nonspecific morpholino control  morpholino mediated srebf2 gene knockdown  nonspecific morpholino control plus daily heat shock in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish  and morpholino mediated srebf2 gene knockdown in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish with daily heatshock. These data provide a resource to study the effects of srebf2 manipulation on transcriptomic responses to optic nerve injury in the adult zebrafish model.", null, null, null, "uninjured 2", "wt L2 M", null, "strain:wild type|age:>3 month|collection date:2023 11 06|geo loc name:USA: Milwaukee  WI|sex:male|tissue:retinal ganglion cell layer|biomaterial provider:Matthew B. Veldman  Medical College of Wisconsin|treatment:uninjured 2|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "LCM seq of Danio rerio:retinal ganglion cell layer", "L02", "L02", "LCM seq  NucleoSpin RNA XS purified  ultra low input RNAseq at Novogene", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA_oligo_dT", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP525334", null, null, "L02_S141_L006_R2_001.fastq.gz L02_S141_L006_R1_001.fastq.gz", "fastq fastq", 24399093702.0, 80791701.0, "L02 S141 L006 R1 001.fastq.gz", "0:151 1:151", "A:6567405598;C:5419524814;G:5626869131;T:6781638199;N:3655960", 151, 151, null, null, 6567405598, 5419524814, 5626869131, 6781638199, 3655960, "SRX25649989", "SRS22293058", "SRA1943640", "Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy", "Medical College of Wisconsin", 2, 0.88324, 0.88428, 0.12546, 0.12616, 0.77141, 0.77021, 0.58483, 0.58455, 151, 151, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-08-09", "Adult", "Adult", "Eye", "Sensory System"], [33402, "SRR30184308", "SRX25649988", "SRS22293057", "SRP525334", "PRJNA1145997", "Regulation of optic nerve regeneration by srebf2", "PRJNA1145997", "Other", "Laser capture microdissection of the retinal ganglion cell layer followed by mRNA seq was used to measure differentially expressed genes in adult zebrafish at three days post optic nerve crush injury. Experimental groups include naive control  nonspecific morpholino control  morpholino mediated srebf2 gene knockdown  nonspecific morpholino control plus daily heat shock in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish  and morpholino mediated srebf2 gene knockdown in Tghsp70l:nSrebf2 2AmCherry  cmlc2:EGFP fish with daily heatshock. These data provide a resource to study the effects of srebf2 manipulation on transcriptomic responses to optic nerve injury in the adult zebrafish model.", null, null, null, "uninjured 1", "wt L1 M", null, "strain:wild type|age:>3 month|collection date:2023 11 06|geo loc name:USA: Milwaukee  WI|sex:male|tissue:retinal ganglion cell layer|biomaterial provider:Matthew B. Veldman  Medical College of Wisconsin|treatment:uninjured 1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "LCM seq of Danio rerio:retinal ganglion cell layer", "L01", "L01", "LCM seq  NucleoSpin RNA XS purified  ultra low input RNAseq at Novogene", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA_oligo_dT", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP525334", null, null, "L01_S123_L005_R2_001.fastq.gz L01_S123_L005_R1_001.fastq.gz", "fastq fastq", 17596481188.0, 58266494.0, "L01 S123 L005 R1 001.fastq.gz", "0:151 1:151", "A:4773806012;C:3941529807;G:3951952531;T:4927955026;N:1237812", 151, 151, null, null, 4773806012, 3941529807, 3951952531, 4927955026, 1237812, "SRX25649988", "SRS22293057", "SRA1943640", "Medical College of Wisconsin|Cell Biology, Neurobiology, and Anatomy", "Medical College of Wisconsin", 2, 0.9124, 0.91249, 0.10052, 0.10034, 0.76751, 0.76773, 0.58532, 0.58581, 151, 151, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-08-09", "Adult", "Adult", "Eye", "Sensory System"], [34109, "SRR31178863", "SRX26561573", "SRS23067068", "SRP542308", "PRJNA1180292", "Zebrafish MAFLD model induced by high fat and high cholesterol diet", "PRJNA1180292", "Other", "Metabolic associated fatty liver disease MAFLD has become one of the most widespread metabolic diseases today  posing a serious threat to human health. In order to further investigate the pathogenesis of MAFLD  explore effective treatment methods and prevention strategies  it is necessary to establish stable and reliable animal models. As a model organism  adult zebrafish has the advantages of high homology with human genes and similar physiological structures  making it an ideal choice for establishing MAFLD models. Therefore  we constructed a zebrafish MAFLD model using high fat and high cholesterol diets 4% and 20%  respectively  and used RNA seq to reveal the transcriptional differences between this model and the liver of zebrafish fed a normal diet. This HFCD zebrafish model can be used for screening and evaluating the efficacy of anti MAFLD drugs", null, null, "4", null, "HFCD1", null, "strain:AB|age:6 mpf|dev stage:Adult|collection date:2021 03 22|geo loc name:China|sex:female|tissue:Liver|Biological duplication:HFCD1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seg of zebrafish: adult female liver", "HFCD1", "HFCD1", "Liver", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA_oligo_dT", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP542308", null, null, "L1EFC230225--NC1.R1.raw.fastq.gz L1EFC230225--NC1.R2.raw.fastq.gz", "fastq fastq", 6794681390.0, 22498945.0, "L1EFC230225  NC1.R1.raw.fastq.gz", "0:151 1:151", "A:1813040924;C:1560264966;G:1623607140;T:1797660733;N:107627", 151, 151, null, null, 1813040924, 1560264966, 1623607140, 1797660733, 107627, "SRX26561573", "SRS23067068", "SRA2001936", "Shandong University of Technology|School of Life Sciences and medicine", "Shandong University of Technology", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2024-10-31", "Adult", "Adult", "Liver", "Liver and Biliary System"], [34110, "SRR31178864", "SRX26561572", "SRS23067067", "SRP542308", "PRJNA1180292", "Zebrafish MAFLD model induced by high fat and high cholesterol diet", "PRJNA1180292", "Other", "Metabolic associated fatty liver disease MAFLD has become one of the most widespread metabolic diseases today  posing a serious threat to human health. In order to further investigate the pathogenesis of MAFLD  explore effective treatment methods and prevention strategies  it is necessary to establish stable and reliable animal models. As a model organism  adult zebrafish has the advantages of high homology with human genes and similar physiological structures  making it an ideal choice for establishing MAFLD models. Therefore  we constructed a zebrafish MAFLD model using high fat and high cholesterol diets 4% and 20%  respectively  and used RNA seq to reveal the transcriptional differences between this model and the liver of zebrafish fed a normal diet. This HFCD zebrafish model can be used for screening and evaluating the efficacy of anti MAFLD drugs", null, null, "3", null, "NC3", null, "strain:AB|age:6 mpf|dev stage:Adult|collection date:2021 03 22|geo loc name:China|sex:female|tissue:Liver|Biological duplication:NC3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seg of zebrafish: adult female liver", "NC3", "NC3", "Liver", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA_oligo_dT", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP542308", null, null, "L1EFC230224--HFCD3.R1.raw.fastq.gz L1EFC230224--HFCD3.R2.raw.fastq.gz", "fastq fastq", 6457281856.0, 21381728.0, "L1EFC230224  HFCD3.R1.raw.fastq.gz", "0:151 1:151", "A:1769303811;C:1446440046;G:1496603676;T:1744830652;N:103671", 151, 151, null, null, 1769303811, 1446440046, 1496603676, 1744830652, 103671, "SRX26561572", "SRS23067067", "SRA2001936", "Shandong University of Technology|School of Life Sciences and medicine", "Shandong University of Technology", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2024-10-31", "Adult", "Adult", "Liver", "Liver and Biliary System"], [34111, "SRR31178865", "SRX26561571", "SRS23067066", "SRP542308", "PRJNA1180292", "Zebrafish MAFLD model induced by high fat and high cholesterol diet", "PRJNA1180292", "Other", "Metabolic associated fatty liver disease MAFLD has become one of the most widespread metabolic diseases today  posing a serious threat to human health. In order to further investigate the pathogenesis of MAFLD  explore effective treatment methods and prevention strategies  it is necessary to establish stable and reliable animal models. As a model organism  adult zebrafish has the advantages of high homology with human genes and similar physiological structures  making it an ideal choice for establishing MAFLD models. Therefore  we constructed a zebrafish MAFLD model using high fat and high cholesterol diets 4% and 20%  respectively  and used RNA seq to reveal the transcriptional differences between this model and the liver of zebrafish fed a normal diet. This HFCD zebrafish model can be used for screening and evaluating the efficacy of anti MAFLD drugs", null, null, "2", null, "NC2", null, "strain:AB|age:6 mpf|dev stage:Adult|collection date:2021 03 22|geo loc name:China|sex:female|tissue:Liver|Biological duplication:NC2|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seg of zebrafish: adult female liver", "NC2", "NC2", "Liver", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA_oligo_dT", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP542308", null, null, "L1EFC230223--HFCD2.R1.raw.fastq.gz L1EFC230223--HFCD2.R2.raw.fastq.gz", "fastq fastq", 6773570382.0, 22429041.0, "L1EFC230223  HFCD2.R1.raw.fastq.gz", "0:151 1:151", "A:1757082060;C:1606989956;G:1661473921;T:1747919214;N:105231", 151, 151, null, null, 1757082060, 1606989956, 1661473921, 1747919214, 105231, "SRX26561571", "SRS23067066", "SRA2001936", "Shandong University of Technology|School of Life Sciences and medicine", "Shandong University of Technology", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2024-10-31", "Adult", "Adult", "Liver", "Liver and Biliary System"], [34112, "SRR31178866", "SRX26561570", "SRS23067065", "SRP542308", "PRJNA1180292", "Zebrafish MAFLD model induced by high fat and high cholesterol diet", "PRJNA1180292", "Other", "Metabolic associated fatty liver disease MAFLD has become one of the most widespread metabolic diseases today  posing a serious threat to human health. In order to further investigate the pathogenesis of MAFLD  explore effective treatment methods and prevention strategies  it is necessary to establish stable and reliable animal models. As a model organism  adult zebrafish has the advantages of high homology with human genes and similar physiological structures  making it an ideal choice for establishing MAFLD models. Therefore  we constructed a zebrafish MAFLD model using high fat and high cholesterol diets 4% and 20%  respectively  and used RNA seq to reveal the transcriptional differences between this model and the liver of zebrafish fed a normal diet. 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