{"database": "metadata", "table": "run_metadata", "is_view": false, "human_description_en": "where experiment.library_selection = \"RANDOM PCR\", experiment.library_source = \"TRANSCRIPTOMIC\" and experiment.library_strategy = \"RNA-Seq\"", "rows": [[10111, "ERR4911024", "ERX4777847", "ERS5435613", "ERP125516", "PRJEB41701", "RNA seq of zebrafish primordial germ cells and somatic cells during early embryogenesis", "E-MTAB-9857", "Transcriptome Analysis", "In this rescue experiment embryos were injected with a Tdrd7 targeting morpholino to block translation of tdrd7 RNA and simultaneously provided with a Tdrd7 morpholino resistant RNA.", "ENA FIRST PUBLIC:2020 12 24|ENA LAST UPDATE:2020 12 02", null, "Protocols: Zebrafish PGCs were isolated via FACS post embryo dissociation. The embryos were grown until the desired stage at 28.5C and collected in synchronous batches. Cell dissociation occurred via pipetting thoroughly up and down the pooled embryos until the solution appeared homogeneous. Yolk excess was removed by two rounds of centrifugation at 300 x g 5 minutes. Cells were passed through a 50 \u03bcm mesh and loaded into a FACS Aria II machine for cell sorting. Morpholino antisense oligos were used in order to inhibit Tdrd7 translation during zebrafish development. Stock morpholinos were diluted in phenol red and about 0.3 pM were injected into the yolk of a fertilised zebrafish embryo. As experimental control a group of embryos were injected with morpholinos having 5 mismatches for the target Tdrd7 transcript. A rescue experiment was performed with Tdrd7 MO resistant RNA from tdrd7 transcript. The RNA was in vitro transcribed and co injected in once cell embryo to rescue MO mediated Tdrd7 KD. Cells were lysed in presence of RNAse inhibitor 0.2 U/\u03bcl and cDNA generated from the cell lysate. cDNA was generated with the Takara SMART Seq\u00ae v4 Ultra\u00ae Low Input RNA Kit as from manufacturer's instruction. In brief reverse transcription was carried out in presence of the SMART Seq CDS Primer II A and SMARTScribeTM Reverse Transcriptase Takara Bio Europe 634889 France. Library amplification occurred according to protocol recommendations and the amplified cDNA purified via the Agencourt AMPure XP beads kit Beckman Coulter A63880 USA.", "PGC Rescue rep2", "SAMEA7678632", "MRC London Institute of Medical Sciences and Faculty of Medicine, Imperial College, London, UK", "ENA first public:2020 12 24|ENA last update:2020 12 02|External Id:SAMEA7678632|INSDC center alias:MRC London Institute of Medical Sciences and Faculty of Medicine Imperial College London UK|INSDC center name:MRC London Institute of Medical Sciences and Faculty of Medicine Imperial College London UK|INSDC first public:2020 12 24T04:05:28Z|INSDC last update:2020 12 02T17:13:26Z|INSDC status:public|Submitter Id:E MTAB 9857:PGC Rescue rep2|age:24|broker name:ArrayExpress|cell type:primordial germ cell|common name:zebrafish|developmental stage:pharyngula prim 5|genotype:tgbuc:gfp|sample name:E MTAB 9857:PGC Rescue rep2|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 550 paired end sequencing; RNA seq of zebrafish primordial germ cells and somatic cells during early embryogenesis", "E MTAB 9857:PGC Rescue rep2 p", "PGC Rescue rep2 p", "RNA seq of zebrafish primordial germ cells and somatic cells during early embryogenesis", "Zebrafish PGCs were isolated via FACS post embryo dissociation. The embryos were grown until the desired stage at 28.5C and collected in synchronous batches. Cell dissociation occurred via pipetting thoroughly up and down the pooled embryos until the solution appeared homogeneous. Yolk excess was removed by two rounds of centrifugation at 300 x g 5 minutes. Cells were passed through a 50 \u03bcm mesh and loaded into a FACS Aria II machine for cell sorting. Morpholino antisense oligos were used in order to inhibit Tdrd7 translation during zebrafish development. Stock morpholinos were diluted in phenol red and about 0.3 pM were injected into the yolk of a fertilised zebrafish embryo. As experimental control a group of embryos were injected with morpholinos having 5 mismatches for the target Tdrd7 transcript. A rescue experiment was performed with Tdrd7 MO resistant RNA from tdrd7 transcript. The RNA was in vitro transcribed and co injected in once cell embryo to rescue MO mediated Tdrd7 KD. Cells were lysed in presence of RNAse inhibitor 0.2 U/\u03bcl and cDNA generated from the cell lysate. cDNA was generated with the Takara SMART Seq\u00ae v4 Ultra\u00ae Low Input RNA Kit as from manufacturer's instruction. In brief reverse transcription was carried out in presence of the SMART Seq CDS Primer II A and SMARTScribeTM Reverse Transcriptase Takara Bio Europe 634889 France. 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The embryos were grown until the desired stage at 28.5C and collected in synchronous batches. Cell dissociation occurred via pipetting thoroughly up and down the pooled embryos until the solution appeared homogeneous. Yolk excess was removed by two rounds of centrifugation at 300 x g 5 minutes. Cells were passed through a 50 \u03bcm mesh and loaded into a FACS Aria II machine for cell sorting. Morpholino antisense oligos were used in order to inhibit Tdrd7 translation during zebrafish development. Stock morpholinos were diluted in phenol red and about 0.3 pM were injected into the yolk of a fertilised zebrafish embryo. As experimental control a group of embryos were injected with morpholinos having 5 mismatches for the target Tdrd7 transcript. A rescue experiment was performed with Tdrd7 MO resistant RNA from tdrd7 transcript. The RNA was in vitro transcribed and co injected in once cell embryo to rescue MO mediated Tdrd7 KD. Cells were lysed in presence of RNAse inhibitor 0.2 U/\u03bcl and cDNA generated from the cell lysate. cDNA was generated with the Takara SMART Seq\u00ae v4 Ultra\u00ae Low Input RNA Kit as from manufacturer's instruction. In brief reverse transcription was carried out in presence of the SMART Seq CDS Primer II A and SMARTScribeTM Reverse Transcriptase Takara Bio Europe 634889 France. Library amplification occurred according to protocol recommendations and the amplified cDNA purified via the Agencourt AMPure XP beads kit Beckman Coulter A63880 USA.", "PGC Rescue rep1", "SAMEA7678631", "MRC London Institute of Medical Sciences and Faculty of Medicine, Imperial College, London, UK", "ENA first public:2020 12 24|ENA last update:2020 12 02|External Id:SAMEA7678631|INSDC center alias:MRC London Institute of Medical Sciences and Faculty of Medicine Imperial College London UK|INSDC center name:MRC London Institute of Medical Sciences and Faculty of Medicine Imperial College London UK|INSDC first public:2020 12 24T04:05:28Z|INSDC last update:2020 12 02T17:13:26Z|INSDC status:public|Submitter Id:E MTAB 9857:PGC Rescue rep1|age:24|broker name:ArrayExpress|cell type:primordial germ cell|common name:zebrafish|developmental stage:pharyngula prim 5|genotype:tgbuc:gfp|sample name:E MTAB 9857:PGC Rescue rep1|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 550 paired end sequencing; RNA seq of zebrafish primordial germ cells and somatic cells during early embryogenesis", "E MTAB 9857:PGC Rescue rep1 p", "PGC Rescue rep1 p", "RNA seq of zebrafish primordial germ cells and somatic cells during early embryogenesis", "Zebrafish PGCs were isolated via FACS post embryo dissociation. The embryos were grown until the desired stage at 28.5C and collected in synchronous batches. Cell dissociation occurred via pipetting thoroughly up and down the pooled embryos until the solution appeared homogeneous. Yolk excess was removed by two rounds of centrifugation at 300 x g 5 minutes. Cells were passed through a 50 \u03bcm mesh and loaded into a FACS Aria II machine for cell sorting. Morpholino antisense oligos were used in order to inhibit Tdrd7 translation during zebrafish development. Stock morpholinos were diluted in phenol red and about 0.3 pM were injected into the yolk of a fertilised zebrafish embryo. As experimental control a group of embryos were injected with morpholinos having 5 mismatches for the target Tdrd7 transcript. A rescue experiment was performed with Tdrd7 MO resistant RNA from tdrd7 transcript. The RNA was in vitro transcribed and co injected in once cell embryo to rescue MO mediated Tdrd7 KD. Cells were lysed in presence of RNAse inhibitor 0.2 U/\u03bcl and cDNA generated from the cell lysate. cDNA was generated with the Takara SMART Seq\u00ae v4 Ultra\u00ae Low Input RNA Kit as from manufacturer's instruction. In brief reverse transcription was carried out in presence of the SMART Seq CDS Primer II A and SMARTScribeTM Reverse Transcriptase Takara Bio Europe 634889 France. Library amplification occurred according to protocol recommendations and the amplified cDNA purified via the Agencourt AMPure XP beads kit Beckman Coulter A63880 USA.", "Experimental Factor: compound:tdrd7 MO + tdrd7 rescue RNA", "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "NextSeq 550", null, "ERP125516", "NextSeq 550 paired end sequencing; RNA seq of zebrafish primordial germ cells and somatic cells during early embryogenesis", "ENA FIRST PUBLIC:2020 12 24|ENA LAST UPDATE:2020 12 09", "Tdrd7_Rescue_PGC_rep1_R1.fastq.gz Tdrd7_Rescue_PGC_rep1_R2.fastq.gz", "fastq fastq", 5952473889.0, 39454929.0, "E MTAB 9857:Tdrd7 Rescue PGC rep1 R", "0:75.44 1:75.43", "A:1640467527;C:1332668425;G:1380033115;T:1597665169;N:1639653", 75, 75, null, null, 1640467527, 1332668425, 1380033115, 1597665169, 1639653, "ERX4777846", "ERS5435612", "ERA3184570", "MRC London Institute of Medical Sciences and Faculty of Medicine, Imperial College, London, UK|European Nucleotide Archive", "MRC London Institute of Medical Sciences and Faculty of Medicine, Imperial College, London, UK|European Nucleotide Archive", 2, 0.95086, 0.95226, 0.06292, 0.0629, 0.70341, 0.70579, 0.46524, 0.46691, 75, 76, "B", "B", "biological fallback assumption", "illumina", "nextseq", "unknown", "random_priming", "unknown", "sc", "single_cell_plate", "smartseq", null, "United Kingdom", "2020-12-02", "Pharyngula", "Embryo", "Whole Organism", "All anatomical structures"], [10113, "ERR4911022", "ERX4777845", "ERS5435611", "ERP125516", "PRJEB41701", "RNA seq of zebrafish primordial germ cells and somatic cells during early embryogenesis", "E-MTAB-9857", "Transcriptome Analysis", "In this rescue experiment embryos were injected with a Tdrd7 targeting morpholino to block translation of tdrd7 RNA and simultaneously provided with a Tdrd7 morpholino resistant RNA.", "ENA FIRST PUBLIC:2020 12 24|ENA LAST UPDATE:2020 12 02", null, "Protocols: Zebrafish PGCs were isolated via FACS post embryo dissociation. The embryos were grown until the desired stage at 28.5C and collected in synchronous batches. Cell dissociation occurred via pipetting thoroughly up and down the pooled embryos until the solution appeared homogeneous. Yolk excess was removed by two rounds of centrifugation at 300 x g 5 minutes. Cells were passed through a 50 \u03bcm mesh and loaded into a FACS Aria II machine for cell sorting. Morpholino antisense oligos were used in order to inhibit Tdrd7 translation during zebrafish development. Stock morpholinos were diluted in phenol red and about 0.3 pM were injected into the yolk of a fertilised zebrafish embryo. As experimental control a group of embryos were injected with morpholinos having 5 mismatches for the target Tdrd7 transcript. A rescue experiment was performed with Tdrd7 MO resistant RNA from tdrd7 transcript. The RNA was in vitro transcribed and co injected in once cell embryo to rescue MO mediated Tdrd7 KD. Cells were lysed in presence of RNAse inhibitor 0.2 U/\u03bcl and cDNA generated from the cell lysate. cDNA was generated with the Takara SMART Seq\u00ae v4 Ultra\u00ae Low Input RNA Kit as from manufacturer's instruction. In brief reverse transcription was carried out in presence of the SMART Seq CDS Primer II A and SMARTScribeTM Reverse Transcriptase Takara Bio Europe 634889 France. Library amplification occurred according to protocol recommendations and the amplified cDNA purified via the Agencourt AMPure XP beads kit Beckman Coulter A63880 USA.", "PGC MO rep2", "SAMEA7678630", "MRC London Institute of Medical Sciences and Faculty of Medicine, Imperial College, London, UK", "ENA first public:2020 12 24|ENA last update:2020 12 02|External Id:SAMEA7678630|INSDC center alias:MRC London Institute of Medical Sciences and Faculty of Medicine Imperial College London UK|INSDC center name:MRC London Institute of Medical Sciences and Faculty of Medicine Imperial College London UK|INSDC first public:2020 12 24T04:05:28Z|INSDC last update:2020 12 02T17:13:26Z|INSDC status:public|Submitter Id:E MTAB 9857:PGC MO rep2|age:24|broker name:ArrayExpress|cell type:primordial germ cell|common name:zebrafish|developmental stage:pharyngula prim 5|genotype:tgbuc:gfp|sample name:E MTAB 9857:PGC MO rep2|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 550 paired end sequencing; RNA seq of zebrafish primordial germ cells and somatic cells during early embryogenesis", "E MTAB 9857:PGC MO rep2 p", "PGC MO rep2 p", "RNA seq of zebrafish primordial germ cells and somatic cells during early embryogenesis", "Zebrafish PGCs were isolated via FACS post embryo dissociation. The embryos were grown until the desired stage at 28.5C and collected in synchronous batches. Cell dissociation occurred via pipetting thoroughly up and down the pooled embryos until the solution appeared homogeneous. Yolk excess was removed by two rounds of centrifugation at 300 x g 5 minutes. Cells were passed through a 50 \u03bcm mesh and loaded into a FACS Aria II machine for cell sorting. Morpholino antisense oligos were used in order to inhibit Tdrd7 translation during zebrafish development. Stock morpholinos were diluted in phenol red and about 0.3 pM were injected into the yolk of a fertilised zebrafish embryo. As experimental control a group of embryos were injected with morpholinos having 5 mismatches for the target Tdrd7 transcript. A rescue experiment was performed with Tdrd7 MO resistant RNA from tdrd7 transcript. The RNA was in vitro transcribed and co injected in once cell embryo to rescue MO mediated Tdrd7 KD. Cells were lysed in presence of RNAse inhibitor 0.2 U/\u03bcl and cDNA generated from the cell lysate. cDNA was generated with the Takara SMART Seq\u00ae v4 Ultra\u00ae Low Input RNA Kit as from manufacturer's instruction. In brief reverse transcription was carried out in presence of the SMART Seq CDS Primer II A and SMARTScribeTM Reverse Transcriptase Takara Bio Europe 634889 France. Library amplification occurred according to protocol recommendations and the amplified cDNA purified via the Agencourt AMPure XP beads kit Beckman Coulter A63880 USA.", "Experimental Factor: compound:tdrd7 targeting MO", "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "NextSeq 550", null, "ERP125516", "NextSeq 550 paired end sequencing; RNA seq of zebrafish primordial germ cells and somatic cells during early embryogenesis", "ENA FIRST PUBLIC:2020 12 24|ENA LAST UPDATE:2020 12 09", "Tdrd7_MO_PGC_rep2_R1.fastq.gz Tdrd7_MO_PGC_rep2_R2.fastq.gz", "fastq fastq", 906443913.0, 6069224.0, "E MTAB 9857:Tdrd7 MO PGC rep2 R", "0:74.67 1:74.68", "A:256935983;C:195137600;G:200975999;T:253026767;N:367564", 74, 74, null, null, 256935983, 195137600, 200975999, 253026767, 367564, "ERX4777845", "ERS5435611", "ERA3184570", "MRC London Institute of Medical Sciences and Faculty of Medicine, Imperial College, London, UK|European Nucleotide Archive", "MRC London Institute of Medical Sciences and Faculty of Medicine, Imperial College, London, UK|European Nucleotide Archive", 2, 0.93468, 0.93603, 0.09796, 0.09848, 0.7119, 0.71439, 0.48682, 0.48933, 75, 76, "B", "B", "biological fallback assumption", "illumina", "nextseq", "unknown", "random_priming", "unknown", "sc", "single_cell_plate", "smartseq", null, "United Kingdom", "2020-12-02", "Pharyngula", "Embryo", "Whole Organism", "All anatomical structures"], [10114, "ERR4911021", "ERX4777844", "ERS5435610", "ERP125516", "PRJEB41701", "RNA seq of zebrafish primordial germ cells and somatic cells during early embryogenesis", "E-MTAB-9857", "Transcriptome Analysis", "In this rescue experiment embryos were injected with a Tdrd7 targeting morpholino to block translation of tdrd7 RNA and simultaneously provided with a Tdrd7 morpholino resistant RNA.", "ENA FIRST PUBLIC:2020 12 24|ENA LAST UPDATE:2020 12 02", null, "Protocols: Zebrafish PGCs were isolated via FACS post embryo dissociation. The embryos were grown until the desired stage at 28.5C and collected in synchronous batches. Cell dissociation occurred via pipetting thoroughly up and down the pooled embryos until the solution appeared homogeneous. Yolk excess was removed by two rounds of centrifugation at 300 x g 5 minutes. Cells were passed through a 50 \u03bcm mesh and loaded into a FACS Aria II machine for cell sorting. Morpholino antisense oligos were used in order to inhibit Tdrd7 translation during zebrafish development. Stock morpholinos were diluted in phenol red and about 0.3 pM were injected into the yolk of a fertilised zebrafish embryo. As experimental control a group of embryos were injected with morpholinos having 5 mismatches for the target Tdrd7 transcript. A rescue experiment was performed with Tdrd7 MO resistant RNA from tdrd7 transcript. The RNA was in vitro transcribed and co injected in once cell embryo to rescue MO mediated Tdrd7 KD. Cells were lysed in presence of RNAse inhibitor 0.2 U/\u03bcl and cDNA generated from the cell lysate. cDNA was generated with the Takara SMART Seq\u00ae v4 Ultra\u00ae Low Input RNA Kit as from manufacturer's instruction. In brief reverse transcription was carried out in presence of the SMART Seq CDS Primer II A and SMARTScribeTM Reverse Transcriptase Takara Bio Europe 634889 France. Library amplification occurred according to protocol recommendations and the amplified cDNA purified via the Agencourt AMPure XP beads kit Beckman Coulter A63880 USA.", "PGC MO rep1", "SAMEA7678629", "MRC London Institute of Medical Sciences and Faculty of Medicine, Imperial College, London, UK", "ENA first public:2020 12 24|ENA last update:2020 12 02|External Id:SAMEA7678629|INSDC center alias:MRC London Institute of Medical Sciences and Faculty of Medicine Imperial College London UK|INSDC center name:MRC London Institute of Medical Sciences and Faculty of Medicine Imperial College London UK|INSDC first public:2020 12 24T04:05:28Z|INSDC last update:2020 12 02T17:13:26Z|INSDC status:public|Submitter Id:E MTAB 9857:PGC MO rep1|age:24|broker name:ArrayExpress|cell type:primordial germ cell|common name:zebrafish|developmental stage:pharyngula prim 5|genotype:tgbuc:gfp|sample name:E MTAB 9857:PGC MO rep1|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 550 paired end sequencing; RNA seq of zebrafish primordial germ cells and somatic cells during early embryogenesis", "E MTAB 9857:PGC MO rep1 p", "PGC MO rep1 p", "RNA seq of zebrafish primordial germ cells and somatic cells during early embryogenesis", "Zebrafish PGCs were isolated via FACS post embryo dissociation. The embryos were grown until the desired stage at 28.5C and collected in synchronous batches. Cell dissociation occurred via pipetting thoroughly up and down the pooled embryos until the solution appeared homogeneous. Yolk excess was removed by two rounds of centrifugation at 300 x g 5 minutes. Cells were passed through a 50 \u03bcm mesh and loaded into a FACS Aria II machine for cell sorting. Morpholino antisense oligos were used in order to inhibit Tdrd7 translation during zebrafish development. Stock morpholinos were diluted in phenol red and about 0.3 pM were injected into the yolk of a fertilised zebrafish embryo. As experimental control a group of embryos were injected with morpholinos having 5 mismatches for the target Tdrd7 transcript. A rescue experiment was performed with Tdrd7 MO resistant RNA from tdrd7 transcript. The RNA was in vitro transcribed and co injected in once cell embryo to rescue MO mediated Tdrd7 KD. Cells were lysed in presence of RNAse inhibitor 0.2 U/\u03bcl and cDNA generated from the cell lysate. cDNA was generated with the Takara SMART Seq\u00ae v4 Ultra\u00ae Low Input RNA Kit as from manufacturer's instruction. In brief reverse transcription was carried out in presence of the SMART Seq CDS Primer II A and SMARTScribeTM Reverse Transcriptase Takara Bio Europe 634889 France. Library amplification occurred according to protocol recommendations and the amplified cDNA purified via the Agencourt AMPure XP beads kit Beckman Coulter A63880 USA.", "Experimental Factor: compound:tdrd7 targeting MO", "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "NextSeq 550", null, "ERP125516", "NextSeq 550 paired end sequencing; RNA seq of zebrafish primordial germ cells and somatic cells during early embryogenesis", "ENA FIRST PUBLIC:2020 12 24|ENA LAST UPDATE:2020 12 09", "Tdrd7_MO_PGC_rep1_R1.fastq.gz Tdrd7_MO_PGC_rep1_R2.fastq.gz", "fastq fastq", 1078919524.0, 7241200.0, "E MTAB 9857:Tdrd7 MO PGC rep1 R", "0:74.50 1:74.50", "A:308200726;C:229836659;G:236649504;T:303673221;N:559414", 74, 74, null, null, 308200726, 229836659, 236649504, 303673221, 559414, "ERX4777844", "ERS5435610", "ERA3184570", "MRC London Institute of Medical Sciences and Faculty of Medicine, Imperial College, London, UK|European Nucleotide Archive", "MRC London Institute of Medical Sciences and Faculty of Medicine, Imperial College, London, UK|European Nucleotide Archive", 2, 0.938, 0.93748, 0.10463, 0.10431, 0.7077, 0.70999, 0.48098, 0.48239, 75, 76, "B", "B", "biological fallback assumption", "illumina", "nextseq", "unknown", "random_priming", "unknown", "sc", "single_cell_plate", "smartseq", null, "United Kingdom", "2020-12-02", "Pharyngula", "Embryo", "Whole Organism", "All anatomical structures"], [10115, "ERR4911020", "ERX4777843", "ERS5435609", "ERP125516", "PRJEB41701", "RNA seq of zebrafish primordial germ cells and somatic cells during early embryogenesis", "E-MTAB-9857", "Transcriptome Analysis", "In this rescue experiment embryos were injected with a Tdrd7 targeting morpholino to block translation of tdrd7 RNA and simultaneously provided with a Tdrd7 morpholino resistant RNA.", "ENA FIRST PUBLIC:2020 12 24|ENA LAST UPDATE:2020 12 02", null, "Protocols: Zebrafish PGCs were isolated via FACS post embryo dissociation. The embryos were grown until the desired stage at 28.5C and collected in synchronous batches. Cell dissociation occurred via pipetting thoroughly up and down the pooled embryos until the solution appeared homogeneous. Yolk excess was removed by two rounds of centrifugation at 300 x g 5 minutes. Cells were passed through a 50 \u03bcm mesh and loaded into a FACS Aria II machine for cell sorting. Morpholino antisense oligos were used in order to inhibit Tdrd7 translation during zebrafish development. Stock morpholinos were diluted in phenol red and about 0.3 pM were injected into the yolk of a fertilised zebrafish embryo. As experimental control a group of embryos were injected with morpholinos having 5 mismatches for the target Tdrd7 transcript. A rescue experiment was performed with Tdrd7 MO resistant RNA from tdrd7 transcript. The RNA was in vitro transcribed and co injected in once cell embryo to rescue MO mediated Tdrd7 KD. Cells were lysed in presence of RNAse inhibitor 0.2 U/\u03bcl and cDNA generated from the cell lysate. cDNA was generated with the Takara SMART Seq\u00ae v4 Ultra\u00ae Low Input RNA Kit as from manufacturer's instruction. In brief reverse transcription was carried out in presence of the SMART Seq CDS Primer II A and SMARTScribeTM Reverse Transcriptase Takara Bio Europe 634889 France. Library amplification occurred according to protocol recommendations and the amplified cDNA purified via the Agencourt AMPure XP beads kit Beckman Coulter A63880 USA.", "PGC 5mm rep2", "SAMEA7678628", "MRC London Institute of Medical Sciences and Faculty of Medicine, Imperial College, London, UK", "ENA first public:2020 12 24|ENA last update:2020 12 02|External Id:SAMEA7678628|INSDC center alias:MRC London Institute of Medical Sciences and Faculty of Medicine Imperial College London UK|INSDC center name:MRC London Institute of Medical Sciences and Faculty of Medicine Imperial College London UK|INSDC first public:2020 12 24T04:05:28Z|INSDC last update:2020 12 02T17:13:26Z|INSDC status:public|Submitter Id:E MTAB 9857:PGC 5mm rep2|age:24|broker name:ArrayExpress|cell type:primordial germ cell|common name:zebrafish|developmental stage:pharyngula prim 5|genotype:tgbuc:gfp|sample name:E MTAB 9857:PGC 5mm rep2|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 550 paired end sequencing; RNA seq of zebrafish primordial germ cells and somatic cells during early embryogenesis", "E MTAB 9857:PGC 5mm rep2 p", "PGC 5mm rep2 p", "RNA seq of zebrafish primordial germ cells and somatic cells during early embryogenesis", "Zebrafish PGCs were isolated via FACS post embryo dissociation. The embryos were grown until the desired stage at 28.5C and collected in synchronous batches. Cell dissociation occurred via pipetting thoroughly up and down the pooled embryos until the solution appeared homogeneous. Yolk excess was removed by two rounds of centrifugation at 300 x g 5 minutes. Cells were passed through a 50 \u03bcm mesh and loaded into a FACS Aria II machine for cell sorting. Morpholino antisense oligos were used in order to inhibit Tdrd7 translation during zebrafish development. Stock morpholinos were diluted in phenol red and about 0.3 pM were injected into the yolk of a fertilised zebrafish embryo. As experimental control a group of embryos were injected with morpholinos having 5 mismatches for the target Tdrd7 transcript. A rescue experiment was performed with Tdrd7 MO resistant RNA from tdrd7 transcript. The RNA was in vitro transcribed and co injected in once cell embryo to rescue MO mediated Tdrd7 KD. Cells were lysed in presence of RNAse inhibitor 0.2 U/\u03bcl and cDNA generated from the cell lysate. cDNA was generated with the Takara SMART Seq\u00ae v4 Ultra\u00ae Low Input RNA Kit as from manufacturer's instruction. In brief reverse transcription was carried out in presence of the SMART Seq CDS Primer II A and SMARTScribeTM Reverse Transcriptase Takara Bio Europe 634889 France. Library amplification occurred according to protocol recommendations and the amplified cDNA purified via the Agencourt AMPure XP beads kit Beckman Coulter A63880 USA.", "Experimental Factor: compound:tdrd7 5mismatch MO", "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "NextSeq 550", null, "ERP125516", "NextSeq 550 paired end sequencing; RNA seq of zebrafish primordial germ cells and somatic cells during early embryogenesis", "ENA FIRST PUBLIC:2020 12 24|ENA LAST UPDATE:2020 12 09", "Tdrd7_5mm_PGC_rep2_R1.fastq.gz Tdrd7_5mm_PGC_rep2_R2.fastq.gz", "fastq fastq", 673911944.0, 4501723.0, "E MTAB 9857:Tdrd7 5mm PGC rep2 R", "0:74.85 1:74.85", "A:184466911;C:150779527;G:155693395;T:182720480;N:251631", 74, 74, null, null, 184466911, 150779527, 155693395, 182720480, 251631, "ERX4777843", "ERS5435609", "ERA3184570", "MRC London Institute of Medical Sciences and Faculty of Medicine, Imperial College, London, UK|European Nucleotide Archive", "MRC London Institute of Medical Sciences and Faculty of Medicine, Imperial College, London, UK|European Nucleotide Archive", 2, 0.91989, 0.92025, 0.08795, 0.08919, 0.70096, 0.70416, 0.49184, 0.49415, 75, 75, "B", "B", "biological fallback assumption", "illumina", "nextseq", "unknown", "random_priming", "unknown", "sc", "single_cell_plate", "smartseq", null, "United Kingdom", "2020-12-02", "Pharyngula", "Embryo", "Whole Organism", "All anatomical structures"], [10116, "ERR4911019", "ERX4777842", "ERS5435608", "ERP125516", "PRJEB41701", "RNA seq of zebrafish primordial germ cells and somatic cells during early embryogenesis", "E-MTAB-9857", "Transcriptome Analysis", "In this rescue experiment embryos were injected with a Tdrd7 targeting morpholino to block translation of tdrd7 RNA and simultaneously provided with a Tdrd7 morpholino resistant RNA.", "ENA FIRST PUBLIC:2020 12 24|ENA LAST UPDATE:2020 12 02", null, "Protocols: Zebrafish PGCs were isolated via FACS post embryo dissociation. The embryos were grown until the desired stage at 28.5C and collected in synchronous batches. Cell dissociation occurred via pipetting thoroughly up and down the pooled embryos until the solution appeared homogeneous. Yolk excess was removed by two rounds of centrifugation at 300 x g 5 minutes. Cells were passed through a 50 \u03bcm mesh and loaded into a FACS Aria II machine for cell sorting. Morpholino antisense oligos were used in order to inhibit Tdrd7 translation during zebrafish development. Stock morpholinos were diluted in phenol red and about 0.3 pM were injected into the yolk of a fertilised zebrafish embryo. As experimental control a group of embryos were injected with morpholinos having 5 mismatches for the target Tdrd7 transcript. A rescue experiment was performed with Tdrd7 MO resistant RNA from tdrd7 transcript. The RNA was in vitro transcribed and co injected in once cell embryo to rescue MO mediated Tdrd7 KD. Cells were lysed in presence of RNAse inhibitor 0.2 U/\u03bcl and cDNA generated from the cell lysate. cDNA was generated with the Takara SMART Seq\u00ae v4 Ultra\u00ae Low Input RNA Kit as from manufacturer's instruction. In brief reverse transcription was carried out in presence of the SMART Seq CDS Primer II A and SMARTScribeTM Reverse Transcriptase Takara Bio Europe 634889 France. Library amplification occurred according to protocol recommendations and the amplified cDNA purified via the Agencourt AMPure XP beads kit Beckman Coulter A63880 USA.", "PGC 5mm rep1", "SAMEA7678627", "MRC London Institute of Medical Sciences and Faculty of Medicine, Imperial College, London, UK", "ENA first public:2020 12 24|ENA last update:2020 12 02|External Id:SAMEA7678627|INSDC center alias:MRC London Institute of Medical Sciences and Faculty of Medicine Imperial College London UK|INSDC center name:MRC London Institute of Medical Sciences and Faculty of Medicine Imperial College London UK|INSDC first public:2020 12 24T04:05:28Z|INSDC last update:2020 12 02T17:13:26Z|INSDC status:public|Submitter Id:E MTAB 9857:PGC 5mm rep1|age:24|broker name:ArrayExpress|cell type:primordial germ cell|common name:zebrafish|developmental stage:pharyngula prim 5|genotype:tgbuc:gfp|sample name:E MTAB 9857:PGC 5mm rep1|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 550 paired end sequencing; RNA seq of zebrafish primordial germ cells and somatic cells during early embryogenesis", "E MTAB 9857:PGC 5mm rep1 p", "PGC 5mm rep1 p", "RNA seq of zebrafish primordial germ cells and somatic cells during early embryogenesis", "Zebrafish PGCs were isolated via FACS post embryo dissociation. The embryos were grown until the desired stage at 28.5C and collected in synchronous batches. Cell dissociation occurred via pipetting thoroughly up and down the pooled embryos until the solution appeared homogeneous. Yolk excess was removed by two rounds of centrifugation at 300 x g 5 minutes. Cells were passed through a 50 \u03bcm mesh and loaded into a FACS Aria II machine for cell sorting. Morpholino antisense oligos were used in order to inhibit Tdrd7 translation during zebrafish development. Stock morpholinos were diluted in phenol red and about 0.3 pM were injected into the yolk of a fertilised zebrafish embryo. As experimental control a group of embryos were injected with morpholinos having 5 mismatches for the target Tdrd7 transcript. A rescue experiment was performed with Tdrd7 MO resistant RNA from tdrd7 transcript. The RNA was in vitro transcribed and co injected in once cell embryo to rescue MO mediated Tdrd7 KD. Cells were lysed in presence of RNAse inhibitor 0.2 U/\u03bcl and cDNA generated from the cell lysate. cDNA was generated with the Takara SMART Seq\u00ae v4 Ultra\u00ae Low Input RNA Kit as from manufacturer's instruction. In brief reverse transcription was carried out in presence of the SMART Seq CDS Primer II A and SMARTScribeTM Reverse Transcriptase Takara Bio Europe 634889 France. Library amplification occurred according to protocol recommendations and the amplified cDNA purified via the Agencourt AMPure XP beads kit Beckman Coulter A63880 USA.", "Experimental Factor: compound:tdrd7 5mismatch MO", "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "NextSeq 550", null, "ERP125516", "NextSeq 550 paired end sequencing; RNA seq of zebrafish primordial germ cells and somatic cells during early embryogenesis", "ENA FIRST PUBLIC:2020 12 24|ENA LAST UPDATE:2020 12 09", "Tdrd7_5mm_PGC_rep1_R1.fastq.gz Tdrd7_5mm_PGC_rep1_R2.fastq.gz", "fastq fastq", 565636692.0, 3782000.0, "E MTAB 9857:Tdrd7 5mm PGC rep1 R", "0:74.78 1:74.78", "A:157353454;C:124504476;G:128260137;T:155314004;N:204621", 74, 74, null, null, 157353454, 124504476, 128260137, 155314004, 204621, "ERX4777842", "ERS5435608", "ERA3184570", "MRC London Institute of Medical Sciences and Faculty of Medicine, Imperial College, London, UK|European Nucleotide Archive", "MRC London Institute of Medical Sciences and Faculty of Medicine, Imperial College, London, UK|European Nucleotide Archive", 2, 0.91957, 0.91995, 0.08301, 0.0845, 0.69929, 0.70195, 0.48544, 0.4877, 76, 76, "B", "B", "biological fallback assumption", "illumina", "nextseq", "unknown", "random_priming", "unknown", "sc", "single_cell_plate", "smartseq", null, "United Kingdom", "2020-12-02", "Pharyngula", "Embryo", "Whole Organism", "All anatomical structures"], [29840, "SRR27586310", "SRX23255332", "SRS20157331", "SRP484115", "PRJNA1065252", "Study of spermine on prednisolone treated zebrafish larvae", "PRJNA1065252", "Other", "RNA sequencing was conducted in zebrafish larvae received prednisolone PN only OP group and PN with spermine SPM group aiming at revealing the key molecular mediating the anti osteoporosis effect of spermine.", null, null, "SPM3", "SPM3", "SPM3", null, "strain:Tubingen line|isolate:not collected|breed:not collected|cultivar:not collected|ecotype:not collected|age:7 day|dev stage:larvae|collection date:2022 11 21|geo loc name:China:Shanghai|sex:not determined|tissue:whole body|birth date:2022 11 14|birth location:China:Shanghai|health state:OP+SPM|sample type:Experimental|treatment:prednisol1+spermine|replicate:biological replicate3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of zebrafish: larvae", "SPM3", "SPM3", "Total RNA was extracted and RNA integrity was assessed by agarose gel electrophoresis purity was checked using a Nanodrop OD260/280 ratio between 1.8 2.2 and quantified with Qubit. The samples were sequenced using the Illumina NovaSeq 6000 platform. The Skewer software trimmed splice sequences and low quality fragments.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP484115", null, null, "SPM3_221124N_S30_L004_R1_001.fastq.gz SPM3_221124N_S30_L004_R2_001.fastq.gz", "fastq fastq", 10885465576.0, 36044588.0, "SPM3 221124N S30 L004 R1 001.fastq.gz", "0:151 1:151", "A:2964982971;C:2446827592;G:2632817265;T:2840605296;N:232452", 151, 151, null, null, 2964982971, 2446827592, 2632817265, 2840605296, 232452, "SRX23255332", "SRS20157331", null, null, "Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2024-01-17", "Larval", "Larval", "Trunk", "Surface Structure"], [29841, "SRR27586311", "SRX23255331", "SRS20157334", "SRP484115", "PRJNA1065252", "Study of spermine on prednisolone treated zebrafish larvae", "PRJNA1065252", "Other", "RNA sequencing was conducted in zebrafish larvae received prednisolone PN only OP group and PN with spermine SPM group aiming at revealing the key molecular mediating the anti osteoporosis effect of spermine.", null, null, "SPM2", "SPM2", "SPM2", null, "strain:Tubingen line|isolate:not collected|breed:not collected|cultivar:not collected|ecotype:not collected|age:7 day|dev stage:larvae|collection date:2022 11 21|geo loc name:China:Shanghai|sex:not determined|tissue:whole body|birth date:2022 11 14|birth location:China:Shanghai|health state:OP+SPM|sample type:Experimental|treatment:prednisol1+spermine|replicate:biological replicate2|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of zebrafish: larvae", "SPM2", "SPM2", "Total RNA was extracted and RNA integrity was assessed by agarose gel electrophoresis purity was checked using a Nanodrop OD260/280 ratio between 1.8 2.2 and quantified with Qubit. The samples were sequenced using the Illumina NovaSeq 6000 platform. The Skewer software trimmed splice sequences and low quality fragments.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP484115", null, null, "SPM2_221124N_S29_L004_R1_001.fastq.gz SPM2_221124N_S29_L004_R2_001.fastq.gz", "fastq fastq", 8024508138.0, 26571219.0, "SPM2 221124N S29 L004 R1 001.fastq.gz", "0:151 1:151", "A:2183023143;C:1799931538;G:1940440841;T:2100940639;N:171977", 151, 151, null, null, 2183023143, 1799931538, 1940440841, 2100940639, 171977, "SRX23255331", "SRS20157334", null, null, "Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2024-01-17", "Larval", "Larval", "Trunk", "Surface Structure"], [29842, "SRR27586312", "SRX23255330", "SRS20157332", "SRP484115", "PRJNA1065252", "Study of spermine on prednisolone treated zebrafish larvae", "PRJNA1065252", "Other", "RNA sequencing was conducted in zebrafish larvae received prednisolone PN only OP group and PN with spermine SPM group aiming at revealing the key molecular mediating the anti osteoporosis effect of spermine.", null, null, "SPM1", "SPM1", "SPM1", null, "strain:Tubingen line|isolate:not collected|breed:not collected|cultivar:not collected|ecotype:not collected|age:7 day|dev stage:larvae|collection date:2022 11 21|geo loc name:China:Shanghai|sex:not determined|tissue:whole body|birth date:2022 11 14|birth location:China:Shanghai|health state:OP+SPM|sample type:Experimental|treatment:prednisol1+spermine|replicate:biological replicate1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of zebrafish: larvae", "SPM1", "SPM1", "Total RNA was extracted and RNA integrity was assessed by agarose gel electrophoresis purity was checked using a Nanodrop OD260/280 ratio between 1.8 2.2 and quantified with Qubit. The samples were sequenced using the Illumina NovaSeq 6000 platform. The Skewer software trimmed splice sequences and low quality fragments.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP484115", null, null, "SPM1_221124N_S28_L004_R1_001.fastq.gz SPM1_221124N_S28_L004_R2_001.fastq.gz", "fastq fastq", 8569531464.0, 28375932.0, "SPM1 221124N S28 L004 R1 001.fastq.gz", "0:151 1:151", "A:2324124676;C:1912685666;G:2084412340;T:2248125150;N:183632", 151, 151, null, null, 2324124676, 1912685666, 2084412340, 2248125150, 183632, "SRX23255330", "SRS20157332", null, null, "Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2024-01-17", "Larval", "Larval", "Trunk", "Surface Structure"], [29843, "SRR27586313", "SRX23255329", "SRS20157333", "SRP484115", "PRJNA1065252", "Study of spermine on prednisolone treated zebrafish larvae", "PRJNA1065252", "Other", "RNA sequencing was conducted in zebrafish larvae received prednisolone PN only OP group and PN with spermine SPM group aiming at revealing the key molecular mediating the anti osteoporosis effect of spermine.", null, null, "OP3", "OP3", "OP3", null, "strain:Tubingen line|isolate:not collected|breed:not collected|cultivar:not collected|ecotype:not collected|age:7 day|dev stage:larvae|collection date:2022 11 21|geo loc name:China:Shanghai|sex:not determined|tissue:whole body|birth date:2022 11 14|birth location:China:Shanghai|health state:OP|sample type:Control|treatment:prednisol1|replicate:biological replicate3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of zebrafish: larvae", "OP3", "OP3", "Total RNA was extracted and RNA integrity was assessed by agarose gel electrophoresis purity was checked using a Nanodrop OD260/280 ratio between 1.8 2.2 and quantified with Qubit. The samples were sequenced using the Illumina NovaSeq 6000 platform. The Skewer software trimmed splice sequences and low quality fragments.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP484115", null, null, "OP3_221124N_S27_L004_R1_001.fastq.gz OP3_221124N_S27_L004_R2_001.fastq.gz", "fastq fastq", 7567534120.0, 25058060.0, "OP3 221124N S27 L004 R1 001.fastq.gz", "0:151 1:151", "A:2053939655;C:1700201614;G:1849434461;T:1963797744;N:160646", 151, 151, null, null, 2053939655, 1700201614, 1849434461, 1963797744, 160646, "SRX23255329", "SRS20157333", null, null, "Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2024-01-17", "Larval", "Larval", "Trunk", "Surface Structure"], [29844, "SRR27586314", "SRX23255328", "SRS20157330", "SRP484115", "PRJNA1065252", "Study of spermine on prednisolone treated zebrafish larvae", "PRJNA1065252", "Other", "RNA sequencing was conducted in zebrafish larvae received prednisolone PN only OP group and PN with spermine SPM group aiming at revealing the key molecular mediating the anti osteoporosis effect of spermine.", null, null, "OP2", "OP2", "OP2", null, "strain:Tubingen line|isolate:not collected|breed:not collected|cultivar:not collected|ecotype:not collected|age:7 day|dev stage:larvae|collection date:2022 11 21|geo loc name:China:Shanghai|sex:not determined|tissue:whole body|birth date:2022 11 14|birth location:China:Shanghai|health state:OP|sample type:Control|treatment:prednisol1|replicate:biological replicate2|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of zebrafish: larvae", "OP2", "OP2", "Total RNA was extracted and RNA integrity was assessed by agarose gel electrophoresis purity was checked using a Nanodrop OD260/280 ratio between 1.8 2.2 and quantified with Qubit. The samples were sequenced using the Illumina NovaSeq 6000 platform. The Skewer software trimmed splice sequences and low quality fragments.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP484115", null, null, "OP2_221124N_S26_L004_R1_001.fastq.gz OP2_221124N_S26_L004_R2_001.fastq.gz", "fastq fastq", 8254140482.0, 27331591.0, "OP2 221124N S26 L004 R1 001.fastq.gz", "0:151 1:151", "A:2224573197;C:1861991388;G:2017457521;T:2149938231;N:180145", 151, 151, null, null, 2224573197, 1861991388, 2017457521, 2149938231, 180145, "SRX23255328", "SRS20157330", null, null, "Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2024-01-17", "Larval", "Larval", "Trunk", "Surface Structure"], [29845, "SRR27586315", "SRX23255327", "SRS20157329", "SRP484115", "PRJNA1065252", "Study of spermine on prednisolone treated zebrafish larvae", "PRJNA1065252", "Other", "RNA sequencing was conducted in zebrafish larvae received prednisolone PN only OP group and PN with spermine SPM group aiming at revealing the key molecular mediating the anti osteoporosis effect of spermine.", null, null, "OP1", "OP1", "OP1", null, "strain:Tubingen line|isolate:not collected|breed:not collected|cultivar:not collected|ecotype:not collected|age:7 day|dev stage:larvae|collection date:2022 11 21|geo loc name:China:Shanghai|sex:not determined|tissue:whole body|birth date:2022 11 14|birth location:China:Shanghai|health state:OP|sample type:Control|treatment:prednisol1|replicate:biological replicate1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of zebrafish: larvae", "OP1", "OP1", "Total RNA was extracted and RNA integrity was assessed by agarose gel electrophoresis purity was checked using a Nanodrop OD260/280 ratio between 1.8 2.2 and quantified with Qubit. The samples were sequenced using the Illumina NovaSeq 6000 platform. The Skewer software trimmed splice sequences and low quality fragments.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP484115", null, null, "OP1_221124N_S25_L004_R1_001.fastq.gz OP1_221124N_S25_L004_R2_001.fastq.gz", "fastq fastq", 8986218380.0, 29755690.0, "OP1 221124N S25 L004 R1 001.fastq.gz", "0:151 1:151", "A:2416582939;C:2017538630;G:2211618173;T:2340284174;N:194464", 151, 151, null, null, 2416582939, 2017538630, 2211618173, 2340284174, 194464, "SRX23255327", "SRS20157329", null, null, "Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2024-01-17", "Larval", "Larval", "Trunk", "Surface Structure"], [32529, "SRR29303005", "SRX24820082", "SRS21534192", "SRP512093", "PRJNA1120586", "Light induce Zebrafish larvae", "PRJNA1120586", "Other", null, null, null, null, null, "L6H 1", null, "breed:zebrafish|cultivar:not applicable|ecotype:AB|age:5 dpf|dev stage:larvae|collection date:2020 10 10|geo loc name:China: Lanzhou|sex:not determined|tissue:whole body|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "rna seq of zebrafish larvae", "L6H 1", "L6H 1", "RNA seq", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "Illumina HiSeq 3000", null, "SRP512093", null, null, "L6H_1_Clean_Data_unaligned.fq.1.gz L6H_1_Clean_Data_unaligned.fq.2.gz", "fastq fastq", 11101739564.0, 40053288.0, "L6H 1 Clean Data unaligned.fq.1.gz", "0:138.59 1:138.59", "A:1881599898;C:3750364237;G:3437926223;T:2031844069;N:5137", 138, 138, null, null, 1881599898, 3750364237, 3437926223, 2031844069, 5137, "SRX24820082", "SRS21534192", "SRA1890517", "Lanzhou University|College of Life Science", "Lanzhou University", 2, 0.97634, 0.97569, 0.11214, 0.10424, 0.76126, 0.76282, 0.50878, 0.71334, 114, 114, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2024-06-06", "Larval", "Larval", "Trunk", "Surface Structure"], [32530, "SRR29303006", "SRX24820081", "SRS21534191", "SRP512093", "PRJNA1120586", "Light induce Zebrafish larvae", "PRJNA1120586", "Other", null, null, null, null, null, "L3H 3", null, "breed:zebrafish|cultivar:not applicable|ecotype:AB|age:5 dpf|dev stage:larvae|collection date:2020 10 09|geo loc name:China: Lanzhou|sex:not determined|tissue:whole body|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "rna seq of zebrafish larvae", "L3H 3", "L3H 3", "RNA seq", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "Illumina HiSeq 3000", null, "SRP512093", null, null, "L3H_3_Clean_Data_unaligned.fq.1.gz 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name:China: Lanzhou|sex:not determined|tissue:whole body|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "rna seq of zebrafish larvae", "L3H 2", "L3H 2", "RNA seq", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "Illumina HiSeq 3000", null, "SRP512093", null, null, "L3H_2_Clean_Data_unaligned.fq.1.gz L3H_2_Clean_Data_unaligned.fq.2.gz", "fastq fastq", 11592698722.0, 41780538.0, "L3H 2 Clean Data unaligned.fq.1.gz", "0:138.74 1:138.73", "A:1962645286;C:3927770539;G:3580608912;T:2121668415;N:5570", 138, 138, null, null, 1962645286, 3927770539, 3580608912, 2121668415, 5570, "SRX24820080", "SRS21534190", "SRA1890517", "Lanzhou University|College of Life Science", "Lanzhou University", 2, 0.97149, 0.9706, 0.10139, 0.09326, 0.76414, 0.76473, 0.71808, 0.69507, 141, 141, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2024-06-06", "Larval", "Larval", "Trunk", "Surface Structure"], [32532, "SRR29303008", "SRX24820079", "SRS21534189", "SRP512093", "PRJNA1120586", "Light induce Zebrafish larvae", "PRJNA1120586", "Other", null, null, null, null, null, "L3H 1", null, "breed:zebrafish|cultivar:not applicable|ecotype:AB|age:5 dpf|dev stage:larvae|collection date:2020 10 07|geo loc name:China: Lanzhou|sex:not determined|tissue:whole body|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "rna seq of zebrafish larvae", "L3H 1", "L3H 1", "RNA seq", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "Illumina HiSeq 3000", null, "SRP512093", null, null, "L3H_1_Clean_Data_unaligned.fq.1.gz L3H_1_Clean_Data_unaligned.fq.2.gz", "fastq fastq", 12497258818.0, 45041676.0, "L3H 1 Clean Data unaligned.fq.1.gz", "0:138.73 1:138.73", "A:2056782475;C:4298181686;G:3906493539;T:2235795303;N:5815", 138, 138, null, null, 2056782475, 4298181686, 3906493539, 2235795303, 5815, "SRX24820079", "SRS21534189", "SRA1890517", "Lanzhou University|College of Life Science", "Lanzhou University", 2, 0.9711, 0.9699, 0.1059, 0.09694, 0.77571, 0.7764, 0.71997, 0.74345, 141, 141, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2024-06-06", "Larval", "Larval", "Trunk", "Surface Structure"], [32533, "SRR29303009", "SRX24820078", "SRS21534188", "SRP512093", "PRJNA1120586", "Light induce Zebrafish larvae", "PRJNA1120586", "Other", null, null, null, null, null, "L1H 3", null, "breed:zebrafish|cultivar:not applicable|ecotype:AB|age:5 dpf|dev stage:larvae|collection date:2020 10 06|geo loc name:China: Lanzhou|sex:not determined|tissue:whole body|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "rna seq of zebrafish larvae", "L1H 3", "L1H 3", "RNA seq", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "Illumina HiSeq 3000", null, "SRP512093", null, null, "L1H_3_Clean_Data_unaligned.fq.1.gz L1H_3_Clean_Data_unaligned.fq.2.gz", "fastq fastq", 12273249812.0, 44192396.0, "L1H 3 Clean Data unaligned.fq.1.gz", "0:138.86 1:138.86", "A:2140693174;C:4064298470;G:3750682721;T:2317568977;N:6470", 138, 138, null, null, 2140693174, 4064298470, 3750682721, 2317568977, 6470, "SRX24820078", "SRS21534188", "SRA1890517", "Lanzhou University|College of Life Science", "Lanzhou University", 2, 0.96617, 0.96354, 0.10751, 0.09938, 0.76258, 0.76347, 0.71559, 0.72025, 141, 141, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2024-06-06", "Larval", "Larval", "Trunk", "Surface Structure"], [32534, "SRR29303010", "SRX24820077", "SRS21534187", "SRP512093", "PRJNA1120586", "Light induce Zebrafish larvae", "PRJNA1120586", "Other", null, null, null, null, null, "L1H 2", null, "breed:zebrafish|cultivar:not applicable|ecotype:AB|age:5 dpf|dev stage:larvae|collection date:2020 10 05|geo loc name:China: Lanzhou|sex:not determined|tissue:whole body|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "rna seq of zebrafish larvae", "L1H 2", "L1H 2", "RNA seq", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "Illumina HiSeq 3000", null, "SRP512093", null, null, "L1H_2_Clean_Data_unaligned.fq.1.gz L1H_2_Clean_Data_unaligned.fq.2.gz", "fastq fastq", 12413369278.0, 44651075.0, "L1H 2 Clean Data unaligned.fq.1.gz", "0:139.01 1:139.00", "A:2070178651;C:4220902189;G:3863965227;T:2258317385;N:5826", 139, 139, null, null, 2070178651, 4220902189, 3863965227, 2258317385, 5826, "SRX24820077", "SRS21534187", "SRA1890517", "Lanzhou University|College of Life Science", "Lanzhou University", 2, 0.97099, 0.96979, 0.08555, 0.07779, 0.77346, 0.77396, 0.73438, 0.55772, 127, 127, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2024-06-06", "Larval", "Larval", "Trunk", "Surface Structure"], [32535, "SRR29303011", "SRX24820076", "SRS21534185", "SRP512093", "PRJNA1120586", "Light induce Zebrafish larvae", "PRJNA1120586", "Other", null, null, null, null, null, "L1H 1", null, "breed:zebrafish|cultivar:not applicable|ecotype:AB|age:5 dpf|dev stage:larvae|collection date:2020 10 04|geo loc name:China: Lanzhou|sex:not determined|tissue:whole body|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "rna seq of zebrafish larvae", "L1H 1", "L1H 1", "RNA seq", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "Illumina HiSeq 3000", null, "SRP512093", null, null, "L1H_1_Clean_Data_unaligned.fq.1.gz L1H_1_Clean_Data_unaligned.fq.2.gz", "fastq fastq", 12302170771.0, 44531982.0, "L1H 1 Clean Data unaligned.fq.1.gz", "0:138.13 1:138.13", "A:2209775658;C:3996913716;G:3742634659;T:2352841220;N:5518", 138, 138, null, null, 2209775658, 3996913716, 3742634659, 2352841220, 5518, "SRX24820076", "SRS21534185", "SRA1890517", "Lanzhou University|College of Life Science", "Lanzhou University", 2, 0.97281, 0.97214, 0.1053, 0.09944, 0.75446, 0.75418, 0.68857, 0.68601, 141, 141, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2024-06-06", "Larval", "Larval", "Trunk", "Surface Structure"], [32536, "SRR29303012", "SRX24820075", "SRS21534186", "SRP512093", "PRJNA1120586", "Light induce Zebrafish larvae", "PRJNA1120586", "Other", null, null, null, null, null, "DD 3", null, "breed:zebrafish|cultivar:not applicable|ecotype:AB|age:5 dpf|dev stage:larvae|collection date:2020 10 03|geo loc name:China: Lanzhou|sex:not determined|tissue:whole body|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "rna seq of zebrafish larvae", "DD 3", "DD 3", "RNA seq", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "Illumina HiSeq 3000", null, "SRP512093", null, null, "DD_3_Clean_Data_unaligned.fq.1.gz DD_3_Clean_Data_unaligned.fq.2.gz", "fastq fastq", 11687586349.0, 42197319.0, "DD 3 Clean Data unaligned.fq.1.gz", "0:138.49 1:138.49", "A:2056950205;C:3858960815;G:3563760225;T:2207909207;N:5897", 138, 138, null, null, 2056950205, 3858960815, 3563760225, 2207909207, 5897, "SRX24820075", "SRS21534186", "SRA1890517", "Lanzhou University|College of Life Science", "Lanzhou University", 2, 0.96463, 0.96389, 0.11454, 0.1079, 0.76163, 0.7626, 0.54192, 0.73007, 141, 141, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2024-06-06", "Larval", "Larval", "Trunk", "Surface Structure"], [32537, "SRR29303013", "SRX24820074", "SRS21534184", "SRP512093", "PRJNA1120586", "Light induce Zebrafish larvae", "PRJNA1120586", "Other", null, null, null, null, null, "L6H 3", null, "breed:zebrafish|cultivar:not applicable|ecotype:AB|age:5 dpf|dev stage:larvae|collection date:2020 10 12|geo loc name:China: Lanzhou|sex:not determined|tissue:whole body|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "rna seq of zebrafish larvae", "L6H 3", "L6H 3", "RNA seq", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "Illumina HiSeq 3000", null, "SRP512093", null, null, "L6H_3_Clean_Data_unaligned.fq.1.gz L6H_3_Clean_Data_unaligned.fq.2.gz", "fastq fastq", 11507362721.0, 41543859.0, "L6H 3 Clean Data unaligned.fq.1.gz", "0:138.50 1:138.49", "A:2101644820;C:3715974381;G:3443971890;T:2245765677;N:5953", 138, 138, null, null, 2101644820, 3715974381, 3443971890, 2245765677, 5953, "SRX24820074", "SRS21534184", "SRA1890517", "Lanzhou University|College of Life Science", "Lanzhou University", 2, 0.96389, 0.96345, 0.12875, 0.12146, 0.75213, 0.7526, 0.69992, 0.69951, 141, 141, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2024-06-06", "Larval", "Larval", "Trunk", "Surface Structure"], [32538, "SRR29303014", "SRX24820073", "SRS21534183", "SRP512093", "PRJNA1120586", "Light induce Zebrafish larvae", "PRJNA1120586", "Other", null, null, null, null, null, "L6H 2", null, "breed:zebrafish|cultivar:not applicable|ecotype:AB|age:5 dpf|dev stage:larvae|collection date:2020 10 11|geo loc name:China: Lanzhou|sex:not determined|tissue:whole body|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "rna seq of zebrafish larvae", "L6H 2", "L6H 2", "RNA seq", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "Illumina HiSeq 3000", null, "SRP512093", null, null, "L6H_2_Clean_Data_unaligned.fq.1.gz L6H_2_Clean_Data_unaligned.fq.2.gz", "fastq fastq", 11424359048.0, 41225621.0, "L6H 2 Clean Data unaligned.fq.1.gz", "0:138.56 1:138.56", "A:1944006759;C:3838938644;G:3536756873;T:2104650999;N:5773", 138, 138, null, null, 1944006759, 3838938644, 3536756873, 2104650999, 5773, "SRX24820073", "SRS21534183", "SRA1890517", "Lanzhou University|College of Life Science", "Lanzhou University", 2, 0.97629, 0.97534, 0.10969, 0.10186, 0.76234, 0.76315, 0.70599, 0.70482, 141, 141, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2024-06-06", "Larval", "Larval", "Trunk", "Surface Structure"], [32539, "SRR29303015", "SRX24820072", "SRS21534182", "SRP512093", "PRJNA1120586", "Light induce Zebrafish larvae", "PRJNA1120586", "Other", null, null, null, null, null, "DD 2", null, "breed:zebrafish|cultivar:not applicable|ecotype:AB|age:5 dpf|dev stage:larvae|collection date:2020 10 02|geo loc name:China: Lanzhou|sex:not determined|tissue:whole body|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "rna seq of zebrafish larvae", "DD 2", "DD 2", "RNA seq", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "Illumina HiSeq 3000", null, "SRP512093", null, null, "DD_2_Clean_Data_unaligned.fq.1.gz DD_2_Clean_Data_unaligned.fq.2.gz", "fastq fastq", 11514098186.0, 41662480.0, "DD 2 Clean Data unaligned.fq.1.gz", "0:138.18 1:138.18", "A:2000101979;C:3836446667;G:3546229881;T:2131314047;N:5612", 138, 138, null, null, 2000101979, 3836446667, 3546229881, 2131314047, 5612, "SRX24820072", "SRS21534182", "SRA1890517", "Lanzhou University|College of Life Science", "Lanzhou University", 2, 0.97366, 0.973, 0.10082, 0.09428, 0.7641, 0.76418, 0.6992, 0.6976, 141, 141, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2024-06-06", "Larval", "Larval", "Trunk", "Surface Structure"], [32540, "SRR29303016", "SRX24820071", "SRS21534181", "SRP512093", "PRJNA1120586", "Light induce Zebrafish larvae", "PRJNA1120586", "Other", null, null, null, null, null, "DD 1", null, "breed:zebrafish|cultivar:not applicable|ecotype:AB|age:5 dpf|dev stage:larvae|collection date:2020 10 01|geo loc name:China: Lanzhou|sex:not determined|tissue:whole body|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "rna seq of zebrafish larvae", "DD 1", "DD 1", "RNA seq", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "Illumina HiSeq 3000", null, "SRP512093", null, null, "DD_1_Clean_Data_unaligned.fq.1.gz DD_1_Clean_Data_unaligned.fq.2.gz", "fastq fastq", 12387599627.0, 44593250.0, "DD 1 Clean Data unaligned.fq.1.gz", "0:138.90 1:138.89", "A:2122330901;C:4173621794;G:3807435168;T:2284205715;N:6049", 138, 138, null, null, 2122330901, 4173621794, 3807435168, 2284205715, 6049, "SRX24820071", "SRS21534181", "SRA1890517", "Lanzhou University|College of Life Science", "Lanzhou University", 2, 0.97122, 0.9708, 0.10985, 0.10105, 0.76384, 0.76406, 0.59526, 0.7234, 141, 141, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2024-06-06", "Larval", "Larval", "Trunk", "Surface Structure"], [34127, "SRR31359854", "SRX26733717", "SRS23223868", "SRP545360", "PRJNA1186345", "The pronephros/cloaca development difference between WT and gdf11 mutants at 24 hpf", "PRJNA1186345", "Other", "To identify specific genes or signaling pathways or genes with altered expression in pronephric ducts and cloaca in the absence of Gdf11 we analyzed transcriptomic changes using RNA deep sequencing on WT and gdf11 mutant embryos in Tgcdh17 dsRed background at 24 hpf.", null, null, null, null, "LJW KV FKDL210002409 1a", null, "strain:LJW KV FKDL210002409 1a|isolate:zebrafish|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:not applicable|collection date:2024 02 18|geo loc name:China: Anhui Medical University Hefei|sex:not applicable|tissue:zebrafish|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "zebrafish mRNA sequencing", "LJW KV FKDL210002409 1a", "LJW KV FKDL210002409 1a", "RNA seq", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "Illumina HiSeq 1000", null, "SRP545360", null, null, "LJW-KV_FKDL210002409-1a_1.clean.fq.gz LJW-KV_FKDL210002409-1a_2.clean.fq.gz", "fastq fastq", 8667883500.0, 28892945.0, "LJW KV FKDL210002409 1a 1.clean.fq.gz", "0:150 1:150", "A:2771307253;C:1399313804;G:1767761135;T:2729383521;N:117787", 150, 150, null, null, 2771307253, 1399313804, 1767761135, 2729383521, 117787, "SRX26733717", "SRS23223868", "SRA2015444", "Anhui Medical University|Basical Medicine", "Anhui Medical University", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2024-11-15", "Undetermined", "Embryo", "Undetermined", "Embryo Imprecise"], [39629, "SRR1947863", "SRX981062", "SRS889614", "SRP056748", "PRJNA279991", "Zebrafish Mespaa regulates miR 430 expression during gastrulation and initiates cardiac laterality", "PRJNA279991", "Other", "Transcriptional events during initial vertebrate heart development in vivo remain poorly understood. Mesp1 a bHLH transcription factor has been described as the earliest transcriptional regulator of cardiac progenitors in multiple species and represents an excellent candidate for the investigation of relevant targets during cardiovascular development. We report here that both depletion and mutation of Mespaa the zebrafish homolog of mammalian Mesp1 lead to randomization of cardiac looping together with significant cardiac morphogenesis defects. These disruptions are preceded by a defect in cardiac left right asymmetry. Surprisingly the defect in asymmetry was found to occur independently of defects in the transient organ of laterality the Kupffer\u2019s vesicle KV. We show that Mespaa regulates miR 430 expression during gastrulation to control the levels of Nodal signaling and that this regulation is required for asymmetric laterality signaling in the prospective heart field. Ectopic expression of miR 430 is sufficient to induce cardiac laterality defects and consistent with Mespaa over expression in this system the reduction of miR 430 leads to cardia bifida. This study reveals a novel transcriptional regulation of miR 430 by Mespaa and a role for this pathway in cardiac laterality during gastrulation.", null, null, null, "Danio rerio injected with mespaa morpholino 5.5hpf", "Zebrafish injected with mespaa morpholino", null, "strain:Ekkwill|dev stage:5.5hpf|sex:pooled male and female|tissue:whole embryo|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Zebrafish Mespaa regulates miR 430 expression during gastrulation and initiates cardiac laterality", "Zebrafish injected with mespaa morpholino replicate 2", "Zebrafish injected with mespaa morpholino replicate 2", "1", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "Illumina HiSeq 2000", null, "SRP056748", null, "loader:latf load", "Mespa-230413_TGTGAA_L008_R2.fastq.gz Mespa-230413_TGTGAA_L008_R1.fastq.gz", "fastq fastq", 7275994449.0, 42132158.0, "RNAseq Danio rerio 5.5hpf mespaa morpholino replicate2", "0:101 1:101", "A:2058072859;C:1476303521;G:1525105443;T:2088915957;N:127596669", 101, 101, null, null, 2058072859, 1476303521, 1525105443, 2088915957, 127596669, "SRX981062", "SRS889614", "SRA249481", "Gladstone Institute|Bioinformatics", "Gladstone Institutes", 2, 0.86631, 0.83649, 0.14484, 0.22396, 0.78861, 0.90727, 0.65055, 0.64516, 101, 101, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "United States", "2015-04-07", "Gastrula", "Embryo", "Whole Organism", "All anatomical structures"], [39630, "SRR1947880", "SRX981061", "SRS895863", "SRP056748", "PRJNA279991", "Zebrafish Mespaa regulates miR 430 expression during gastrulation and initiates cardiac laterality", "PRJNA279991", "Other", "Transcriptional events during initial vertebrate heart development in vivo remain poorly understood. Mesp1 a bHLH transcription factor has been described as the earliest transcriptional regulator of cardiac progenitors in multiple species and represents an excellent candidate for the investigation of relevant targets during cardiovascular development. We report here that both depletion and mutation of Mespaa the zebrafish homolog of mammalian Mesp1 lead to randomization of cardiac looping together with significant cardiac morphogenesis defects. These disruptions are preceded by a defect in cardiac left right asymmetry. Surprisingly the defect in asymmetry was found to occur independently of defects in the transient organ of laterality the Kupffer\u2019s vesicle KV. We show that Mespaa regulates miR 430 expression during gastrulation to control the levels of Nodal signaling and that this regulation is required for asymmetric laterality signaling in the prospective heart field. Ectopic expression of miR 430 is sufficient to induce cardiac laterality defects and consistent with Mespaa over expression in this system the reduction of miR 430 leads to cardia bifida. This study reveals a novel transcriptional regulation of miR 430 by Mespaa and a role for this pathway in cardiac laterality during gastrulation.", null, null, null, "Danio rerio injected with negative control morpholino 5.5hpf", "Zebrafish injected with untargeted negative control morpholino", null, "strain:Ekkwill|dev stage:5.5 hpf|sex:pooled male and female|tissue:whole embryo|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Zebrafish Mespaa regulates miR 430 expression during gastrulation and initiates cardiac laterality", "Zebrafish injected with untargeted negative control morpholino replicate 2", "Zebrafish injected with untargeted negative control morpholino replicate 2", "1", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "Illumina HiSeq 2000", null, "SRP056748", null, "loader:latf load", "Std-230413_GTGTTA_L008_R1.fastq.gz Std-230413_GTGTTA_L008_R2.fastq.gz", "fastq fastq", 10015452496.0, 49581448.0, "RNAseq Danio rerio 5.5hpf negative control morpholino replicate2", "0:101 1:101", "A:2883382922;C:1998043392;G:2046542450;T:2937071125;N:150412607", 101, 101, null, null, 2883382922, 1998043392, 2046542450, 2937071125, 150412607, "SRX981061", "SRS895863", "SRA249481", "Gladstone Institute|Bioinformatics", "Gladstone Institutes", 2, 0.8766, 0.85002, 0.17158, 0.25762, 0.77167, 0.89057, 0.64584, 0.66342, 101, 101, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "United States", "2016-03-31", "Gastrula", "Embryo", "Whole Organism", "All anatomical structures"], [39631, "SRR1947876", "SRX981011", "SRS895863", "SRP056748", "PRJNA279991", "Zebrafish Mespaa regulates miR 430 expression during gastrulation and initiates cardiac laterality", "PRJNA279991", "Other", "Transcriptional events during initial vertebrate heart development in vivo remain poorly understood. Mesp1 a bHLH transcription factor has been described as the earliest transcriptional regulator of cardiac progenitors in multiple species and represents an excellent candidate for the investigation of relevant targets during cardiovascular development. We report here that both depletion and mutation of Mespaa the zebrafish homolog of mammalian Mesp1 lead to randomization of cardiac looping together with significant cardiac morphogenesis defects. These disruptions are preceded by a defect in cardiac left right asymmetry. Surprisingly the defect in asymmetry was found to occur independently of defects in the transient organ of laterality the Kupffer\u2019s vesicle KV. We show that Mespaa regulates miR 430 expression during gastrulation to control the levels of Nodal signaling and that this regulation is required for asymmetric laterality signaling in the prospective heart field. Ectopic expression of miR 430 is sufficient to induce cardiac laterality defects and consistent with Mespaa over expression in this system the reduction of miR 430 leads to cardia bifida. This study reveals a novel transcriptional regulation of miR 430 by Mespaa and a role for this pathway in cardiac laterality during gastrulation.", null, null, null, "Danio rerio injected with negative control morpholino 5.5hpf", "Zebrafish injected with untargeted negative control morpholino", null, "strain:Ekkwill|dev stage:5.5 hpf|sex:pooled male and female|tissue:whole embryo|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Zebrafish Mespaa regulates miR 430 expression during gastrulation and initiates cardiac laterality", "Zebrafish injected with untargeted negative control morpholino", "Zebrafish injected with untargeted negative control morpholino replicate 1", "1", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "Illumina HiSeq 2000", null, "SRP056748", null, "loader:latf load", "Std-190413_ACACGA_L008_R2.fastq.gz Std-190413_ACACGA_L008_R1.fastq.gz", "fastq fastq", 10412449358.0, 51546779.0, "RNAseq Danio rerio 5.5hpf negative control morpholino replicate1", "0:101 1:101", "A:2998996009;C:2064995108;G:2125498634;T:3066600855;N:156358752", 101, 101, null, null, 2998996009, 2064995108, 2125498634, 3066600855, 156358752, "SRX981011", "SRS895863", "SRA249481", "Gladstone Institute|Bioinformatics", "Gladstone Institutes", 2, 0.87394, 0.84802, 0.15672, 0.23447, 0.77072, 0.89049, 0.63241, 0.65533, 101, 101, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "United States", "2016-03-31", "Gastrula", "Embryo", "Whole Organism", "All anatomical structures"], [39632, "SRR1947862", "SRX974390", "SRS889614", "SRP056748", "PRJNA279991", "Zebrafish Mespaa regulates miR 430 expression during gastrulation and initiates cardiac laterality", "PRJNA279991", "Other", "Transcriptional events during initial vertebrate heart development in vivo remain poorly understood. Mesp1 a bHLH transcription factor has been described as the earliest transcriptional regulator of cardiac progenitors in multiple species and represents an excellent candidate for the investigation of relevant targets during cardiovascular development. We report here that both depletion and mutation of Mespaa the zebrafish homolog of mammalian Mesp1 lead to randomization of cardiac looping together with significant cardiac morphogenesis defects. These disruptions are preceded by a defect in cardiac left right asymmetry. Surprisingly the defect in asymmetry was found to occur independently of defects in the transient organ of laterality the Kupffer\u2019s vesicle KV. We show that Mespaa regulates miR 430 expression during gastrulation to control the levels of Nodal signaling and that this regulation is required for asymmetric laterality signaling in the prospective heart field. Ectopic expression of miR 430 is sufficient to induce cardiac laterality defects and consistent with Mespaa over expression in this system the reduction of miR 430 leads to cardia bifida. This study reveals a novel transcriptional regulation of miR 430 by Mespaa and a role for this pathway in cardiac laterality during gastrulation.", null, null, null, "Danio rerio injected with mespaa morpholino 5.5hpf", "Zebrafish injected with mespaa morpholino", null, "strain:Ekkwill|dev stage:5.5hpf|sex:pooled male and female|tissue:whole embryo|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Zebrafish Mespaa regulates miR 430 expression during gastrulation and initiates cardiac laterality", "RNAseq analysis of mespaa knockdown embryos", "Zebrafish injected with mespaa morpholino replicate 1", "1", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "Illumina HiSeq 2000", "1000Application ReadForward1", "SRP056748", null, "loader:latf load", "Mespa-190413_CACACA_L008_R2.fastq.gz Mespa-190413_CACACA_L008_R1.fastq.gz", "fastq fastq", 715965164.0, 3668538.0, "RNAseq Danio rerio 5.5hpf mespaa morpholino replicate1", "0:101 1:101", "A:203464307;C:144071219;G:148845038;T:209200873;N:10383727", 101, 101, null, null, 203464307, 144071219, 148845038, 209200873, 10383727, "SRX974390", "SRS889614", "SRA249481", "Gladstone Institute|Bioinformatics", "Gladstone Institutes", 2, 0.86222, 0.8307, 0.14165, 0.17545, 0.7624, 0.84415, 0.65454, 0.64758, 101, 101, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "United States", "2015-04-07", "Gastrula", "Embryo", "Whole Organism", "All anatomical structures"], [41309, "SRR4242454", "SRX2163334", "SRS1691357", "SRP089876", "PRJNA341782", "Danio rerio Phenotype or Genotype", "PRJNA341782", "Other", "Based on the differential comparison of transcriptomes of Homo hetero zygote Het and wild type Wt in vivo protein trap mutagenesis system we have produced series of expression codex of the zebrafish. Here we reported the transcriptomic characteristic of a line with stable deficits found in homozygous Homo expressing the strongest signal of red fluorescent protein mRFP in the central neural system and vascular system.", null, null, null, null, "ho 160", null, "strain:TU|isolate:homozygous|age:3 dpf|dev stage:3 dpf|sex:not determined|tissue:whole fish|biomaterial provider:Intitute of Medical Sciences University of Toronto Toronto Canada. Xiao yan WEN|birth date:2015 05 01|genotype:homozygous|phenotype:small head abnormal vascular system|treatment:in vivo protein trap mutagenesis|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "1", "ho 160", "ho 160", "1", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "SINGLE", "ILLUMINA", "Illumina HiSeq 2000", null, "SRP089876", null, null, "HO.fq.gz", "fastq", 568688414.0, 11605886.0, "HO.fq.gz", "0:49", "A:146833011;C:136838940;G:142141532;T:142831489;N:43442", 49, null, null, null, 146833011, 136838940, 142141532, 142831489, 43442, "SRX2163334", "SRS1691357", "SRA471230", "Guangdong Ocean University|Fisheries College", "Guangdong Ocean University", 1, 0.92072, null, 0.03662, null, 0.79584, null, 0.46051, null, 49, null, "B", null, "usable mapping rate", "illumina", "hiseq_era", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2016-09-14", "Larval", "Larval", "Whole Organism", "All anatomical structures"], [41310, "SRR4242453", "SRX2163333", "SRS1691356", "SRP089876", "PRJNA341782", "Danio rerio Phenotype or Genotype", "PRJNA341782", "Other", "Based on the differential comparison of transcriptomes of Homo hetero zygote Het and wild type Wt in vivo protein trap mutagenesis system we have produced series of expression codex of the zebrafish. Here we reported the transcriptomic characteristic of a line with stable deficits found in homozygous Homo expressing the strongest signal of red fluorescent protein mRFP in the central neural system and vascular system.", null, null, null, null, "he 160", null, "strain:TU|isolate:heterozygous|age:3 dpf|dev stage:3 dpf|sex:not determined|tissue:whole fish|biomaterial provider:Intitute of Medical Sciences University of Toronto Toronto Canada. Xiao yan WEN|birth date:2015 05 01|genotype:heterozygous|phenotype:normal|treatment:in vivo protein trap mutagenesis|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "1", "he 160", "he 160", "1", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "SINGLE", "ILLUMINA", "Illumina HiSeq 2000", null, "SRP089876", null, null, "HE.fq", "fastq", 578835432.0, 11812968.0, "HE.fq", "0:49", "A:149797321;C:137638814;G:143656076;T:147699087;N:44134", 49, null, null, null, 149797321, 137638814, 143656076, 147699087, 44134, "SRX2163333", "SRS1691356", "SRA471230", "Guangdong Ocean University|Fisheries College", "Guangdong Ocean University", 1, 0.96392, null, 0.03868, null, 0.71064, null, 0.48997, null, 49, null, "B", null, "usable mapping rate", "illumina", "hiseq_era", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2016-09-19", "Larval", "Larval", "Whole Organism", "All anatomical structures"], [41311, "SRR4242452", "SRX2163332", "SRS1691355", "SRP089876", "PRJNA341782", "Danio rerio Phenotype or Genotype", "PRJNA341782", "Other", "Based on the differential comparison of transcriptomes of Homo hetero zygote Het and wild type Wt in vivo protein trap mutagenesis system we have produced series of expression codex of the zebrafish. Here we reported the transcriptomic characteristic of a line with stable deficits found in homozygous Homo expressing the strongest signal of red fluorescent protein mRFP in the central neural system and vascular system.", null, null, null, null, "wt 160", null, "strain:TU|isolate:wild type|age:3 dpf|dev stage:3 dpf|sex:not determined|tissue:whole fish|biomaterial provider:Intitute of Medical Sciences University of Toronto Toronto Canada. Xiao yan WEN|birth date:2015 05 01|genotype:wild type|phenotype:normal|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "1", "wt 160", "wt 160", "1", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "SINGLE", "ILLUMINA", "Illumina HiSeq 2000", null, "SRP089876", null, null, "WT.fq.gz", "fastq", 582208151.0, 11881799.0, "WT.fq.gz", "0:49", "A:151848733;C:138129346;G:143758419;T:148430704;N:40949", 49, null, null, null, 151848733, 138129346, 143758419, 148430704, 40949, "SRX2163332", "SRS1691355", "SRA471230", "Guangdong Ocean University|Fisheries College", "Guangdong Ocean University", 1, 0.95342, null, 0.04301, null, 0.75503, null, 0.48726, null, 49, null, "B", null, "usable mapping rate", "illumina", "hiseq_era", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2016-09-19", "Larval", "Larval", "Whole Organism", "All anatomical structures"], [41349, "SRR4330940", "SRX2205508", "SRS1723862", "SRP090717", "PRJNA345071", "Transcriptome sequencing of zebrafish mutants", "PRJNA345071", "Other", "To investigate the possible mechanism that NOD1 impacts larval survival in zebrafish we performed transcriptome analysis to explore NOD1 related signaling pathways. Zebrafish larvae from WT and NOD1 1IS / were collected at 10 dpf and used for transcriptome sequencing.", null, null, null, null, "ZIS 3", null, "strain:mutant biological replicate 3|dev stage:10 dpf|sex:pooled male and female|tissue:larvae|biomaterial provider:Chang MX|birth date:2015 08|breeding method:?artificial insemination|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA seq of zebrafish mutant at 10 dpf", "ZIS 3", "ZIS 3", "1", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "SRP090717", null, null, "ZIS-3_S41_L003_R1_001.fastq ZIS-3_S41_L003_R2_001.fastq", "fastq fastq", 8951074036.0, 29639318.0, "ZIS 3 S41 L003 R1 001.fastq", "0:151 1:151", "A:2283207699;C:2181203331;G:2250920956;T:2233298555;N:2443495", 151, 151, null, null, 2283207699, 2181203331, 2250920956, 2233298555, 2443495, "SRX2205508", "SRS1723862", "SRA481515", "Institute of Hydrobiology, Chinese Academy of Sciences|Center for Fish Biology and Fishery Biotechnology", "Institute of Hydrobiology, Chinese Academy of Sciences", 2, 0.94568, 0.94599, 0.04792, 0.04705, 0.68511, 0.69191, 0.48349, 0.49441, 151, 151, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2019-12-31", "Larval", "Larval", "Whole Organism", "All anatomical structures"], [41350, "SRR4330939", "SRX2205507", "SRS1723861", "SRP090717", "PRJNA345071", "Transcriptome sequencing of zebrafish mutants", "PRJNA345071", "Other", "To investigate the possible mechanism that NOD1 impacts larval survival in zebrafish we performed transcriptome analysis to explore NOD1 related signaling pathways. Zebrafish larvae from WT and NOD1 1IS / were collected at 10 dpf and used for transcriptome sequencing.", null, null, null, null, "ZIS 2", null, "strain:mutant biological replicate 2|dev stage:10 dpf|sex:pooled male and female|tissue:larvae|biomaterial provider:Chang MX|birth date:2015 08|breeding method:?artificial insemination|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA seq of zebrafish mutant at 10 dpf", "ZIS 2", "ZIS 2", "1", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "SRP090717", null, null, "ZIS-2_S40_L003_R1_001.fastq ZIS-2_S40_L003_R2_001.fastq", "fastq fastq", 9435991208.0, 31245004.0, "ZIS 2 S40 L003 R2 001.fastq", "0:151 1:151", "A:2432777029;C:2274473737;G:2351228268;T:2374883671;N:2628503", 151, 151, null, null, 2432777029, 2274473737, 2351228268, 2374883671, 2628503, "SRX2205507", "SRS1723861", "SRA481515", "Institute of Hydrobiology, Chinese Academy of Sciences|Center for Fish Biology and Fishery Biotechnology", "Institute of Hydrobiology, Chinese Academy of Sciences", 2, 0.93852, 0.93895, 0.06814, 0.06752, 0.67464, 0.68185, 0.4731, 0.48737, 151, 151, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2019-12-31", "Larval", "Larval", "Whole Organism", "All anatomical structures"], [41351, "SRR4330938", "SRX2205506", "SRS1723859", "SRP090717", "PRJNA345071", "Transcriptome sequencing of zebrafish mutants", "PRJNA345071", "Other", "To investigate the possible mechanism that NOD1 impacts larval survival in zebrafish we performed transcriptome analysis to explore NOD1 related signaling pathways. Zebrafish larvae from WT and NOD1 1IS / were collected at 10 dpf and used for transcriptome sequencing.", null, null, null, null, "ZIS 1", null, "strain:mutant biological replicate 1|dev stage:10 dpf|sex:pooled male and female|tissue:larvae|biomaterial provider:Chang MX|birth date:2015 08|breeding method:?artificial insemination|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA seq of zebrafish mutant at 10 dpf", "ZIS 1", "ZIS 1", "1", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "SRP090717", null, null, "ZIS-1_S58_L004_R1_001.fastq ZIS-1_S58_L004_R2_001.fastq", "fastq fastq", 9474125956.0, 31371278.0, "ZIS 1 S58 L004 R1 001.fastq", "0:151 1:151", "A:2432127105;C:2294541938;G:2369906198;T:2374445988;N:3104727", 151, 151, null, null, 2432127105, 2294541938, 2369906198, 2374445988, 3104727, "SRX2205506", "SRS1723859", "SRA481515", "Institute of Hydrobiology, Chinese Academy of Sciences|Center for Fish Biology and Fishery Biotechnology", "Institute of Hydrobiology, Chinese Academy of Sciences", 2, 0.94217, 0.94136, 0.05812, 0.05769, 0.67957, 0.68665, 0.48986, 0.48912, 151, 151, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2019-12-31", "Larval", "Larval", "Whole Organism", "All anatomical structures"], [41352, "SRR4330937", "SRX2205505", "SRS1723863", "SRP090717", "PRJNA345071", "Transcriptome sequencing of zebrafish mutants", "PRJNA345071", "Other", "To investigate the possible mechanism that NOD1 impacts larval survival in zebrafish we performed transcriptome analysis to explore NOD1 related signaling pathways. Zebrafish larvae from WT and NOD1 1IS / were collected at 10 dpf and used for transcriptome sequencing.", null, null, null, null, "WT 3", null, "strain:wild type biological replicate 3|dev stage:10 dpf|sex:pooled male and female|tissue:larvae|biomaterial provider:Chang MX|birth date:2015 08|breeding method:?artificial insemination|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA seq of zebrafish wildtype at 10 dpf", "WT 3", "WT 3", "1", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "SRP090717", null, null, "WT-3_S59_L004_R1_001.fastq WT-3_S59_L004_R2_001.fastq", "fastq fastq", 9459728710.0, 31323605.0, "WT 3 S59 L004 R2 001.fastq", "0:151 1:151", "A:2435834973;C:2283457751;G:2353515662;T:2383834638;N:3085686", 151, 151, null, null, 2435834973, 2283457751, 2353515662, 2383834638, 3085686, "SRX2205505", "SRS1723863", "SRA481515", "Institute of Hydrobiology, Chinese Academy of Sciences|Center for Fish Biology and Fishery Biotechnology", "Institute of Hydrobiology, Chinese Academy of Sciences", 2, 0.93703, 0.93671, 0.06932, 0.06902, 0.67815, 0.68527, 0.49406, 0.48629, 151, 151, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2019-12-31", "Larval", "Larval", "Whole Organism", "All anatomical structures"], [41353, "SRR4330936", "SRX2205504", "SRS1723860", "SRP090717", "PRJNA345071", "Transcriptome sequencing of zebrafish mutants", "PRJNA345071", "Other", "To investigate the possible mechanism that NOD1 impacts larval survival in zebrafish we performed transcriptome analysis to explore NOD1 related signaling pathways. Zebrafish larvae from WT and NOD1 1IS / were collected at 10 dpf and used for transcriptome sequencing.", null, null, null, null, "WT 2", null, "strain:wild type biological replicate 2|dev stage:10 dpf|sex:pooled male and female|tissue:larvae|biomaterial provider:Chang MX|birth date:2015 08|breeding method:?artificial insemination|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA seq of zebrafish wildtype at 10 dpf", "WT 2", "WT 2", "1", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "SRP090717", null, null, "WT-2_S39_L003_R1_001.fastq WT-2_S39_L003_R2_001.fastq", "fastq fastq", 8115257628.0, 26871714.0, "WT 2 S39 L003 R1 001.fastq", "0:151 1:151", "A:2056302105;C:1991691115;G:2061808328;T:2003236611;N:2219469", 151, 151, null, null, 2056302105, 1991691115, 2061808328, 2003236611, 2219469, "SRX2205504", "SRS1723860", "SRA481515", "Institute of Hydrobiology, Chinese Academy of Sciences|Center for Fish Biology and Fishery Biotechnology", "Institute of Hydrobiology, Chinese Academy of Sciences", 2, 0.9479, 0.94848, 0.03505, 0.0349, 0.69946, 0.70638, 0.4871, 0.48737, 151, 151, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2019-12-31", "Larval", "Larval", "Whole Organism", "All anatomical structures"], [41354, "SRR4330935", "SRX2205503", "SRS1723858", "SRP090717", "PRJNA345071", "Transcriptome sequencing of zebrafish mutants", "PRJNA345071", "Other", "To investigate the possible mechanism that NOD1 impacts larval survival in zebrafish we performed transcriptome analysis to explore NOD1 related signaling pathways. Zebrafish larvae from WT and NOD1 1IS / were collected at 10 dpf and used for transcriptome sequencing.", null, null, null, null, "WT 1", null, "strain:wild type biological replicate 1|dev stage:10 dpf|sex:pooled male and female|tissue:larvae|biomaterial provider:Chang MX|birth date:2015 08|breeding method:?artificial insemination|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA seq of zebrafish wildtype at 10 dpf", "WT 1", "WT 1", "To investigate the possible mechanism that NOD1 impacts larval survival in zebrafish we performed transcriptome analysis to explore NOD1 related signaling pathways. Zebrafish larvae from WT and NOD1 1IS / were collected at 10 dpf and used for transcriptome sequencing.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "SRP090717", null, null, "WT-1_S38_L003_R1_001.fastq WT-1_S38_L003_R2_001.fastq", "fastq fastq", 9771991274.0, 32357587.0, "WT 1 S38 L003 R2 001.fastq", "0:151 1:151", "A:2524417259;C:2348903783;G:2421563752;T:2474419170;N:2687310", 151, 151, null, null, 2524417259, 2348903783, 2421563752, 2474419170, 2687310, "SRX2205503", "SRS1723858", "SRA481515", "Institute of Hydrobiology, Chinese Academy of Sciences|Center for Fish Biology and Fishery Biotechnology", "Institute of Hydrobiology, Chinese Academy of Sciences", 2, 0.93787, 0.93896, 0.0697, 0.06907, 0.67446, 0.68162, 0.49385, 0.49554, 151, 151, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2019-12-31", "Larval", "Larval", "Whole Organism", "All anatomical structures"], [41603, "SRR5086607", "SRX2403900", "SRS1843204", "SRP094738", "PRJNA356682", "Transcriptome sequencing of zebrafish mutants with or without xxx tarda infection", "PRJNA356682", "Other", "To investigate the possible mechanism that NOD1/RIP2 impacts larval survival in zebrafish under infectious condition we performed transcriptome analysis. Zebrafish larvae from WT NOD1 / and RIP / were infected with Edwardsiella tarda and collected at 24 hours post infection hpi.", null, null, null, null, "RIP2 Etarda 2", null, "isolate:RIP2 mutant biological replicate 2|age:7 dpf|dev stage:larvae|sex:pooled male and female|tissue:larvae|biomaterial provider:Chang MX|birth date:2016 3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA seq of zebrafish RIP2 mutant at 7 dpf", "RIP2 Etarda 2", "RIP2 Etarda 2", "1", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "SRP094738", null, null, "S231_07B_CHG009012-0413lane6-RP-E-tarda-2_L003_R2.fastq.gz S231_07B_CHG009012-0413lane6-RP-E-tarda-2_L003_R1.fastq.gz", "fastq fastq", 12903564000.0, 43011880.0, "S231 07B CHG009012 0413lane6 RP E tarda 2 L003 R1.fastq.gz", "0:150 1:150", "A:3540121379;C:2905716861;G:3027503729;T:3429809429;N:412602", 150, 150, null, null, 3540121379, 2905716861, 3027503729, 3429809429, 412602, "SRX2403900", "SRS1843204", "SRA501516", "Institute of Hydrobiology, Chinese Academy of Sciences|Center for Fish Biology and Fishery Biotechnology", "Institute of Hydrobiology, Chinese Academy of Sciences", 2, 0.91895, 0.92448, 0.22816, 0.22865, 0.6873, 0.69104, 0.59821, 0.59683, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2016-12-08", "Larval", "Larval", "Whole Organism", "All anatomical structures"], [41604, "SRR5086606", "SRX2403899", "SRS1843203", "SRP094738", "PRJNA356682", "Transcriptome sequencing of zebrafish mutants with or without xxx tarda infection", "PRJNA356682", "Other", "To investigate the possible mechanism that NOD1/RIP2 impacts larval survival in zebrafish under infectious condition we performed transcriptome analysis. Zebrafish larvae from WT NOD1 / and RIP / were infected with Edwardsiella tarda and collected at 24 hours post infection hpi.", null, null, null, null, "NOD1 Etarda 1", null, "isolate:NOD1 mutant biological replicate 1|age:7 dpf|dev stage:larvae|sex:pooled male and female|tissue:larvae|biomaterial provider:Chang MX|birth date:2016 3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA seq of zebrafish NOD1 mutant at 7 dpf", "NOD1 Etarda 1", "NOD1 Etarda 1", "1", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "SRP094738", null, null, "S231_07B_CHG009012-0413lane6-ND-E-tarda-1_L003_R1.fastq.gz S231_07B_CHG009012-0413lane6-ND-E-tarda-1_L003_R2.fastq.gz", "fastq fastq", 12320572500.0, 41068575.0, "S231 07B CHG009012 0413lane6 ND E tarda 1 L003 R1.fastq.gz", "0:150 1:150", "A:3355646078;C:2797387103;G:2920550779;T:3246590603;N:397937", 150, 150, null, null, 3355646078, 2797387103, 2920550779, 3246590603, 397937, "SRX2403899", "SRS1843203", "SRA501516", "Institute of Hydrobiology, Chinese Academy of Sciences|Center for Fish Biology and Fishery Biotechnology", "Institute of Hydrobiology, Chinese Academy of Sciences", 2, 0.86604, 0.87043, 0.20071, 0.20146, 0.69369, 0.69869, 0.5907, 0.59843, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2020-11-27", "Larval", "Larval", "Whole Organism", "All anatomical structures"], [41605, "SRR5086605", "SRX2403898", "SRS1843202", "SRP094738", "PRJNA356682", "Transcriptome sequencing of zebrafish mutants with or without xxx tarda infection", "PRJNA356682", "Other", "To investigate the possible mechanism that NOD1/RIP2 impacts larval survival in zebrafish under infectious condition we performed transcriptome analysis. Zebrafish larvae from WT NOD1 / and RIP / were infected with Edwardsiella tarda and collected at 24 hours post infection hpi.", null, null, null, null, "NOD1 Etarda 2", null, "isolate:NOD1 mutant biological replicate 2|age:7 dpf|dev stage:larvae|sex:pooled male and female|tissue:larvae|biomaterial provider:Chang MX|birth date:2016 3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA seq of zebrafish NOD1 mutant at 7 dpf", "NOD1 Etarda 2", "NOD1 Etarda 2", "1", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "SRP094738", null, null, "S231_07B_CHG009012-0413lane6-ND-E-tarda-2_L003_R1.fastq.gz S231_07B_CHG009012-0413lane6-ND-E-tarda-2_L003_R2.fastq.gz", "fastq fastq", 15551299800.0, 51837666.0, "S231 07B CHG009012 0413lane6 ND E tarda 2 L003 R1.fastq.gz", "0:150 1:150", "A:4265070757;C:3495006741;G:3648563581;T:4142124454;N:534267", 150, 150, null, null, 4265070757, 3495006741, 3648563581, 4142124454, 534267, "SRX2403898", "SRS1843202", "SRA501516", "Institute of Hydrobiology, Chinese Academy of Sciences|Center for Fish Biology and Fishery Biotechnology", "Institute of Hydrobiology, Chinese Academy of Sciences", 2, 0.86759, 0.86834, 0.23528, 0.23586, 0.68779, 0.69272, 0.57804, 0.57769, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2020-11-27", "Larval", "Larval", "Whole Organism", "All anatomical structures"], [41606, "SRR5086604", "SRX2403897", "SRS1843201", "SRP094738", "PRJNA356682", "Transcriptome sequencing of zebrafish mutants with or without xxx tarda infection", "PRJNA356682", "Other", "To investigate the possible mechanism that NOD1/RIP2 impacts larval survival in zebrafish under infectious condition we performed transcriptome analysis. Zebrafish larvae from WT NOD1 / and RIP / were infected with Edwardsiella tarda and collected at 24 hours post infection hpi.", null, null, null, null, "RIP2 Etarda 3", null, "isolate:RIP2 mutant biological replicate 3|age:7 dpf|dev stage:larvae|sex:pooled male and female|tissue:larvae|biomaterial provider:Chang MX|birth date:2016 3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA seq of zebrafish RIP2 mutant at 7 dpf", "RIP2 Etarda 3", "RIP2 Etarda 3", "1", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "SRP094738", null, null, "S231_07B_CHG009012-0413lane6-RP-E-tarda-3_L003_R1.fastq.gz S231_07B_CHG009012-0413lane6-RP-E-tarda-3_L003_R2.fastq.gz", "fastq fastq", 12144058200.0, 40480194.0, "S231 07B CHG009012 0413lane6 RP E tarda 3 L003 R1.fastq.gz", "0:150 1:150", "A:3277198817;C:2779538400;G:2897367228;T:3189540397;N:413358", 150, 150, null, null, 3277198817, 2779538400, 2897367228, 3189540397, 413358, "SRX2403897", "SRS1843201", "SRA501516", "Institute of Hydrobiology, Chinese Academy of Sciences|Center for Fish Biology and Fishery Biotechnology", "Institute of Hydrobiology, Chinese Academy of Sciences", 2, 0.91654, 0.9175, 0.25593, 0.25637, 0.68795, 0.69414, 0.56468, 0.56806, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2020-11-27", "Larval", "Larval", "Whole Organism", "All anatomical structures"], [41607, "SRR5086603", "SRX2403896", "SRS1843200", "SRP094738", "PRJNA356682", "Transcriptome sequencing of zebrafish mutants with or without xxx tarda infection", "PRJNA356682", "Other", "To investigate the possible mechanism that NOD1/RIP2 impacts larval survival in zebrafish under infectious condition we performed transcriptome analysis. Zebrafish larvae from WT NOD1 / and RIP / were infected with Edwardsiella tarda and collected at 24 hours post infection hpi.", null, null, null, null, "WT Etarda 3", null, "isolate:wild type biological replicate 3|age:7 dpf|dev stage:larvae|sex:pooled male and female|tissue:larvae|biomaterial provider:Chang MX|birth date:2016 3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA seq of zebrafish wildtype at 7 dpf", "WT Etarda 3", "WT Etarda 3", "1", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "SRP094738", null, null, "S231_07B_CHG009012-0413lane6-WT-E-tarda-3_L003_R1.fastq.gz S231_07B_CHG009012-0413lane6-WT-E-tarda-3_L003_R2.fastq.gz", "fastq fastq", 13158784200.0, 43862614.0, "S231 07B CHG009012 0413lane6 WT E tarda 3 L003 R1.fastq.gz", "0:150 1:150", "A:3492740553;C:3076792456;G:3216533074;T:3372287751;N:430366", 150, 150, null, null, 3492740553, 3076792456, 3216533074, 3372287751, 430366, "SRX2403896", "SRS1843200", "SRA501516", "Institute of Hydrobiology, Chinese Academy of Sciences|Center for Fish Biology and Fishery Biotechnology", "Institute of Hydrobiology, Chinese Academy of Sciences", 2, 0.91602, 0.92416, 0.23246, 0.23514, 0.68164, 0.68663, 0.55407, 0.55215, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2020-11-27", "Larval", "Larval", "Whole Organism", "All anatomical structures"], [41608, "SRR5086602", "SRX2403895", "SRS1843199", "SRP094738", "PRJNA356682", "Transcriptome sequencing of zebrafish mutants with or without xxx tarda infection", "PRJNA356682", "Other", "To investigate the possible mechanism that NOD1/RIP2 impacts larval survival in zebrafish under infectious condition we performed transcriptome analysis. Zebrafish larvae from WT NOD1 / and RIP / were infected with Edwardsiella tarda and collected at 24 hours post infection hpi.", null, null, null, null, "WT Etarda 1", null, "isolate:wild type biological replicate 1|age:7 dpf|dev stage:larvae|sex:pooled male and female|tissue:larvae|biomaterial provider:Chang MX|birth date:2016 3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA seq of zebrafish wildtype at 7dpf", "WT Etarda 1", "WT Etarda 1", "To investigate the possible mechanism that NOD1/RIP2 impacts larval survival in zebrafish under infectious condition we performed transcriptome analysis. Zebrafish larvae from WT NOD1 / and RIP / were infected with Edwardsiella tarda and collected at 24 hours post infection hpi.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "SRP094738", null, null, "S231_07B_CHG009012-0413lane6-WT-E-tarda-1_L003_R1.fastq.gz S231_07B_CHG009012-0413lane6-WT-E-tarda-1_L003_R2.fastq.gz", "fastq fastq", 11991766500.0, 39972555.0, "S231 07B CHG009012 0413lane6 WT E tarda 1 L003 R1.fastq.gz", "0:150 1:150", "A:3282107515;C:2699757677;G:2810716102;T:3198780035;N:405171", 150, 150, null, null, 3282107515, 2699757677, 2810716102, 3198780035, 405171, "SRX2403895", "SRS1843199", "SRA501516", "Institute of Hydrobiology, Chinese Academy of Sciences|Center for Fish Biology and Fishery Biotechnology", "Institute of Hydrobiology, Chinese Academy of Sciences", 2, 0.91731, 0.91778, 0.23094, 0.23042, 0.6784, 0.68596, 0.59413, 0.59241, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2020-11-27", "Larval", "Larval", "Whole Organism", "All anatomical structures"], [41609, "SRR5086601", "SRX2403894", "SRS1843198", "SRP094738", "PRJNA356682", "Transcriptome sequencing of zebrafish mutants with or without xxx tarda infection", "PRJNA356682", "Other", "To investigate the possible mechanism that NOD1/RIP2 impacts larval survival in zebrafish under infectious condition we performed transcriptome analysis. Zebrafish larvae from WT NOD1 / and RIP / were infected with Edwardsiella tarda and collected at 24 hours post infection hpi.", null, null, null, null, "NOD1 Etarda 3", null, "isolate:NOD1 mutant biological replicate 3|age:7 dpf|dev stage:larvae|sex:pooled male and female|tissue:larvae|biomaterial provider:Chang MX|birth date:2016 3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA seq of zebrafish NOD1 mutant at 7 dpf", "NOD1 Etarda 3", "NOD1 Etarda 3", "1", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "SRP094738", null, null, "S231_07B_CHG009012-0413lane6-ND-E-tarda-3_L003_R1.fastq.gz S231_07B_CHG009012-0413lane6-ND-E-tarda-3_L003_R2.fastq.gz", "fastq fastq", 13231011300.0, 44103371.0, "S231 07B CHG009012 0413lane6 ND E tarda 3 L003 R1.fastq.gz", "0:150 1:150", "A:3583948856;C:3022120866;G:3144073054;T:3480438095;N:430429", 150, 150, null, null, 3583948856, 3022120866, 3144073054, 3480438095, 430429, "SRX2403894", "SRS1843198", "SRA501516", "Institute of Hydrobiology, Chinese Academy of Sciences|Center for Fish Biology and Fishery Biotechnology", "Institute of Hydrobiology, Chinese Academy of Sciences", 2, 0.8625, 0.86327, 0.21095, 0.21123, 0.69126, 0.69808, 0.59807, 0.59873, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2020-11-27", "Larval", "Larval", "Whole Organism", "All anatomical structures"], [41610, "SRR5086600", "SRX2403893", "SRS1843197", "SRP094738", "PRJNA356682", "Transcriptome sequencing of zebrafish mutants with or without xxx tarda infection", "PRJNA356682", "Other", "To investigate the possible mechanism that NOD1/RIP2 impacts larval survival in zebrafish under infectious condition we performed transcriptome analysis. Zebrafish larvae from WT NOD1 / and RIP / were infected with Edwardsiella tarda and collected at 24 hours post infection hpi.", null, null, null, null, "WT Etarda 2", null, "isolate:wild type biological replicate 2|age:7 dpf|dev stage:larvae|sex:pooled male and female|tissue:larvae|biomaterial provider:Chang MX|birth date:2016 3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA seq of zebrafish wildtype at 7 dpf", "WT Etarda 2", "WT Etarda 2", "1", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "SRP094738", null, null, "S231_07B_CHG009012-0413lane6-WT-E-tarda-2_L003_R1.fastq.gz S231_07B_CHG009012-0413lane6-WT-E-tarda-2_L003_R2.fastq.gz", "fastq fastq", 11385997200.0, 37953324.0, "S231 07B CHG009012 0413lane6 WT E tarda 2 L003 R1.fastq.gz", "0:150 1:150", "A:3100666193;C:2581361422;G:2676308702;T:3027299840;N:361043", 150, 150, null, null, 3100666193, 2581361422, 2676308702, 3027299840, 361043, "SRX2403893", "SRS1843197", "SRA501516", "Institute of Hydrobiology, Chinese Academy of Sciences|Center for Fish Biology and Fishery Biotechnology", "Institute of Hydrobiology, Chinese Academy of Sciences", 2, 0.91592, 0.91843, 0.23215, 0.23276, 0.67894, 0.6843, 0.56756, 0.57405, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2020-11-27", "Larval", "Larval", "Whole Organism", "All anatomical structures"], [41611, "SRR5086599", "SRX2403892", "SRS1843196", "SRP094738", "PRJNA356682", "Transcriptome sequencing of zebrafish mutants with or without xxx tarda infection", "PRJNA356682", "Other", "To investigate the possible mechanism that NOD1/RIP2 impacts larval survival in zebrafish under infectious condition we performed transcriptome analysis. Zebrafish larvae from WT NOD1 / and RIP / were infected with Edwardsiella tarda and collected at 24 hours post infection hpi.", null, null, null, null, "RIP2 Etarda 1", null, "isolate:RIP2 mutant biological replicate 1|age:7 dpf|dev stage:larvae|sex:pooled male and female|tissue:larvae|biomaterial provider:Chang MX|birth date:2016 3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA seq of zebrafish RIP2 mutant at 7 dpf", "RIP2 Etarda 1", "RIP2 Etarda 1", "1", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "SRP094738", null, null, "S231_07B_CHG009012-0413lane6-RP-E-tarda-1_L003_R1.fastq.gz S231_07B_CHG009012-0413lane6-RP-E-tarda-1_L003_R2.fastq.gz", "fastq fastq", 12517987200.0, 41726624.0, "S231 07B CHG009012 0413lane6 RP E tarda 1 L003 R1.fastq.gz", "0:150 1:150", "A:3383702176;C:2864897363;G:2969958296;T:3299042555;N:386810", 150, 150, null, null, 3383702176, 2864897363, 2969958296, 3299042555, 386810, "SRX2403892", "SRS1843196", "SRA501516", "Institute of Hydrobiology, Chinese Academy of Sciences|Center for Fish Biology and Fishery Biotechnology", "Institute of Hydrobiology, Chinese Academy of Sciences", 2, 0.92847, 0.93046, 0.20764, 0.20908, 0.68217, 0.68889, 0.58846, 0.59063, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2020-11-27", "Larval", "Larval", "Whole Organism", "All anatomical structures"], [41754, "SRR5131065", "SRX2444929", "SRS1878801", "SRP095651", "PRJNA358793", "Transcriptome sequencing of zebrafish RIP2 mutants", "PRJNA358793", "Other", "Zebrafish larvae from wildtype and RIP2 / were collected at 7 dpf and used for transcriptome sequencing.", null, null, null, null, "control 3", null, "isolate:wild type biological replicate 3|age:7 dpf|dev stage:larvae|sex:pooled male and female|tissue:larvae|biomaterial provider:Chang MX|birth date:2016 3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA seq of zebrafish wildtype at 7 dpf", "control 3", "control 3", "1", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "SRP095651", null, null, "S231_07B_CHG009010-0413lane4-WT-3_L001_R2.fastq.gz S231_07B_CHG009010-0413lane4-WT-3_L001_R1.fastq.gz", "fastq fastq", 7170870300.0, 23902901.0, "S231 07B CHG009010 0413lane4 WT 3 L001 R2.fastq.gz", "0:150 1:150", "A:1861223343;C:1713993670;G:1720465419;T:1875067010;N:120858", 150, 150, null, null, 1861223343, 1713993670, 1720465419, 1875067010, 120858, "SRX2444929", "SRS1878801", "SRA510115", "Institute of Hydrobiology, Chinese Academy of Sciences|Center for Fish Biology and Fishery Biotechnology", "Institute of Hydrobiology, Chinese Academy of Sciences", 2, 0.93719, 0.93331, 0.07804, 0.07734, 0.66478, 0.67152, 0.48991, 0.48846, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2016-12-25", "Larval", "Larval", "Whole Organism", "All anatomical structures"], [41755, "SRR5131064", "SRX2444928", "SRS1878800", "SRP095651", "PRJNA358793", "Transcriptome sequencing of zebrafish RIP2 mutants", "PRJNA358793", "Other", "Zebrafish larvae from wildtype and RIP2 / were collected at 7 dpf and used for transcriptome sequencing.", null, null, null, null, "RP 3", null, "isolate:RIP2 mutant biological replicate 3|age:7 dpf|dev stage:larvae|sex:pooled male and female|tissue:larvae|biomaterial provider:Chang MX|birth date:2016 3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA seq of zebrafish RIP2 mutant at 7 dpf", "RP 3", "RP 3", "1", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "SRP095651", null, null, "S231_07B_CHG009010-0413lane4-RP-3_L001_R1.fastq.gz S231_07B_CHG009010-0413lane4-RP-3_L001_R2.fastq.gz", "fastq fastq", 7468205400.0, 24894018.0, "S231 07B CHG009010 0413lane4 RP 3 L001 R1.fastq.gz", "0:150 1:150", "A:1923116988;C:1800729614;G:1804880487;T:1939349442;N:128869", 150, 150, null, null, 1923116988, 1800729614, 1804880487, 1939349442, 128869, "SRX2444928", "SRS1878800", "SRA510115", "Institute of Hydrobiology, Chinese Academy of Sciences|Center for Fish Biology and Fishery Biotechnology", "Institute of Hydrobiology, Chinese Academy of Sciences", 2, 0.94182, 0.93791, 0.07003, 0.06911, 0.67391, 0.68032, 0.48725, 0.48383, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2020-12-21", "Larval", "Larval", "Whole Organism", "All anatomical structures"], [41756, "SRR5131063", "SRX2444927", "SRS1878799", "SRP095651", "PRJNA358793", "Transcriptome sequencing of zebrafish RIP2 mutants", "PRJNA358793", "Other", "Zebrafish larvae from wildtype and RIP2 / were collected at 7 dpf and used for transcriptome sequencing.", null, null, null, null, "control 1", null, "isolate:wild type biological replicate 1|age:7 dpf|dev stage:larvae|sex:pooled male and female|tissue:larvae|biomaterial provider:Chang MX|birth date:2016 3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA seq of zebrafish wildtype at 7dpf", "control 1", "control 1", "To investigate the possible mechanism that RIP2 impacts immune response in zebrafish we performed transcriptome analysis. Zebrafish larvae from WT and RIP / were collected at 7 dpf.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "SRP095651", null, null, "S231_07B_CHG009010-0413lane4-WT-1_L001_R1.fastq.gz S231_07B_CHG009010-0413lane4-WT-1_L001_R2.fastq.gz", "fastq fastq", 7794774600.0, 25982582.0, "S231 07B CHG009010 0413lane4 WT 1 L001 R1.fastq.gz", "0:150 1:150", "A:2030478380;C:1854310335;G:1862519444;T:2047324790;N:141651", 150, 150, null, null, 2030478380, 1854310335, 1862519444, 2047324790, 141651, "SRX2444927", "SRS1878799", "SRA510115", "Institute of Hydrobiology, Chinese Academy of Sciences|Center for Fish Biology and Fishery Biotechnology", "Institute of Hydrobiology, Chinese Academy of Sciences", 2, 0.94149, 0.93653, 0.07296, 0.07218, 0.66614, 0.67294, 0.49615, 0.48813, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2020-12-21", "Larval", "Larval", "Whole Organism", "All anatomical structures"], [41757, "SRR5131062", "SRX2444926", "SRS1878798", "SRP095651", "PRJNA358793", "Transcriptome sequencing of zebrafish RIP2 mutants", "PRJNA358793", "Other", "Zebrafish larvae from wildtype and RIP2 / were collected at 7 dpf and used for transcriptome sequencing.", null, null, null, null, "control 2", null, "isolate:wild type biological replicate 2|age:7 dpf|dev stage:larvae|sex:pooled male and female|tissue:larvae|biomaterial provider:Chang MX|birth date:2016 3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA seq of zebrafish wildtype at 7 dpf", "control 2", "control 2", "1", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "SRP095651", null, null, "S231_07B_CHG009010-0413lane4-WT-2_L001_R1.fastq.gz S231_07B_CHG009010-0413lane4-WT-2_L001_R2.fastq.gz", "fastq fastq", 5623326600.0, 18744422.0, "S231 07B CHG009010 0413lane4 WT 2 L001 R2.fastq.gz", "0:150 1:150", "A:1451164902;C:1350358314;G:1359170147;T:1462531546;N:101691", 150, 150, null, null, 1451164902, 1350358314, 1359170147, 1462531546, 101691, "SRX2444926", "SRS1878798", "SRA510115", "Institute of Hydrobiology, Chinese Academy of Sciences|Center for Fish Biology and Fishery Biotechnology", "Institute of Hydrobiology, Chinese Academy of Sciences", 2, 0.93864, 0.93904, 0.06797, 0.06755, 0.67048, 0.67521, 0.49371, 0.48819, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2020-12-21", "Larval", "Larval", "Whole Organism", "All anatomical structures"], [41758, "SRR5131061", "SRX2444925", "SRS1878797", "SRP095651", "PRJNA358793", "Transcriptome sequencing of zebrafish RIP2 mutants", "PRJNA358793", "Other", "Zebrafish larvae from wildtype and RIP2 / were collected at 7 dpf and used for transcriptome sequencing.", null, null, null, null, "RP 1", null, "isolate:RIP2 mutant biological replicate 1|age:7 dpf|dev stage:larvae|sex:pooled male and female|tissue:larvae|biomaterial provider:Chang MX|birth date:2016 3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA seq of zebrafish RIP2 mutant at 7 dpf", "RP 1", "RP 1", "1", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "SRP095651", null, null, "S231_07B_CHG009010-0413lane4-RP-1_L001_R1.fastq.gz S231_07B_CHG009010-0413lane4-RP-1_L001_R2.fastq.gz", "fastq fastq", 8583762600.0, 28612542.0, "S231 07B CHG009010 0413lane4 RP 1 L001 R2.fastq.gz", "0:150 1:150", "A:2233488442;C:2047436003;G:2053585249;T:2249097620;N:155286", 150, 150, null, null, 2233488442, 2047436003, 2053585249, 2249097620, 155286, "SRX2444925", "SRS1878797", "SRA510115", "Institute of Hydrobiology, Chinese Academy of Sciences|Center for Fish Biology and Fishery Biotechnology", "Institute of Hydrobiology, Chinese Academy of Sciences", 2, 0.94045, 0.93559, 0.08356, 0.0825, 0.66576, 0.67188, 0.48456, 0.48745, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2020-12-21", "Larval", "Larval", "Whole Organism", "All anatomical structures"], [41759, "SRR5131060", "SRX2444924", "SRS1878796", "SRP095651", "PRJNA358793", "Transcriptome sequencing of zebrafish RIP2 mutants", "PRJNA358793", "Other", "Zebrafish larvae from wildtype and RIP2 / were collected at 7 dpf and used for transcriptome sequencing.", null, null, null, null, "RP 2", null, "isolate:RIP2 mutant biological replicate 2|age:7 dpf|dev stage:larvae|sex:pooled male and female|tissue:larvae|biomaterial provider:Chang MX|birth date:2016 3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA seq of zebrafish RIP2 mutant at 7 dpf", "RP 2", "RP 2", "1", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "SRP095651", null, null, "S231_07B_CHG009010-0413lane4-RP-2_L001_R1.fastq.gz S231_07B_CHG009010-0413lane4-RP-2_L001_R2.fastq.gz", "fastq fastq", 6660884700.0, 22202949.0, "S231 07B CHG009010 0413lane4 RP 2 L001 R1.fastq.gz", "0:150 1:150", "A:1716524391;C:1604826566;G:1610050455;T:1729364340;N:118948", 150, 150, null, null, 1716524391, 1604826566, 1610050455, 1729364340, 118948, "SRX2444924", "SRS1878796", "SRA510115", "Institute of Hydrobiology, Chinese Academy of Sciences|Center for Fish Biology and Fishery Biotechnology", "Institute of Hydrobiology, Chinese Academy of Sciences", 2, 0.94509, 0.94069, 0.07191, 0.0702, 0.67322, 0.68045, 0.4937, 0.4864, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2020-12-21", "Larval", "Larval", "Whole Organism", "All anatomical structures"], [44932, "SRR6308290", "SRX3408682", "SRS2701478", "SRP125291", "PRJNA419036", "Danio rerio strain:ASWT Transcriptome or Gene expression", "PRJNA419036", "Other", "A genome wide map of circular RNA in adult zebrafish.", null, null, null, "Zf gills", "Zf gills", null, "strain:ASWT|age:1year|sex:male|tissue:Gills|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "A genome wide map of circular RNA in adult zebrafish", "Zf gills", "Zf gills", "Tissue were isolated from adult zebrafish anaesthetized using 0.004% Tricaine Sigma USA. Extreme care was taken to avoid contamination to obtain pure homogenous tissue samples. The tissues were repeatedly washed in PBS to remove contaminating debris. The tissue samples were homogenized in Trizol for cell lysis Invitrogen USA. RNA was isolated from the homogenized tissue samples using RNeasy kit Qiagen USA. Sample preparation for sequencing was carried out using Truseq stranded RNA sample preparation kit Illumina USA as per supplier's instructions. In order to remove the ribosomal RNA rRNA one microgram of total RNA was hybridised with Ribo zero gold rRNA removal probe. Upon removing rRNA the samples were processed for fragmentation in the presence of ionic cations at 37 degree Celcius. First stranded complementary DNA cDNA was prepared by random hexamers and superscript II reverse transcriptase Invitrogen USA in presence of Actinomycin D to facilitate RNA dependent synthesis for improving strand specificity. The second strand was synthesised with second strand cDNA mix containing dUTP instead of dTTP and subjected to A base addition followed by adapter ligation. Final libraries were prepared by amplifying adapter ligated double strand cDNA. Clusters were generated on Hiseq flow cell v3 Illumina in cBot according to standard protocol Illumina USA.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP125291", null, null, "Zf_gills_R1.fastq Zf_gills_R2.fastq", "fastq fastq", 4496743848.0, 22524306.0, "Zf gills R2.fastq", "0:99.98 1:99.65", "A:1187180280;C:1050024773;G:1063872181;T:1195629658;N:36956", 99, 99, null, null, 1187180280, 1050024773, 1063872181, 1195629658, 36956, "SRX3408682", "SRS2701478", "SRA631861", "CSIR-Institute of Genomics and Integrative Biology|Genomic Medicine", "CSIR-Institute of Genomics and Integrative Biology", 2, 0.79541, 0.79749, 0.21897, 0.21816, 0.68952, 0.68947, 0.50966, 0.51356, 101, 101, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "ribozero", "bulk", "unknown", "unknown", null, "India", "2017-11-20", "Adult", "Adult", "Gill", "Respiratory System"], [44933, "SRR6308291", "SRX3408681", "SRS2701477", "SRP125291", "PRJNA419036", "Danio rerio strain:ASWT Transcriptome or Gene expression", "PRJNA419036", "Other", "A genome wide map of circular RNA in adult zebrafish.", null, null, null, "Zf heart", "Zf heart", null, "strain:ASWT|age:1year|sex:male|tissue:Heart|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "A genome wide map of circular RNA in adult zebrafish", "Zf heart", "Zf heart", "Tissue were isolated from adult zebrafish anaesthetized using 0.004% Tricaine Sigma USA. Extreme care was taken to avoid contamination to obtain pure homogenous tissue samples. The tissues were repeatedly washed in PBS to remove contaminating debris. The tissue samples were homogenized in Trizol for cell lysis Invitrogen USA. RNA was isolated from the homogenized tissue samples using RNeasy kit Qiagen USA. Sample preparation for sequencing was carried out using Truseq stranded RNA sample preparation kit Illumina USA as per supplier's instructions. In order to remove the ribosomal RNA rRNA one microgram of total RNA was hybridised with Ribo zero gold rRNA removal probe. Upon removing rRNA the samples were processed for fragmentation in the presence of ionic cations at 37 degree Celcius. First stranded complementary DNA cDNA was prepared by random hexamers and superscript II reverse transcriptase Invitrogen USA in presence of Actinomycin D to facilitate RNA dependent synthesis for improving strand specificity. The second strand was synthesised with second strand cDNA mix containing dUTP instead of dTTP and subjected to A base addition followed by adapter ligation. Final libraries were prepared by amplifying adapter ligated double strand cDNA. Clusters were generated on Hiseq flow cell v3 Illumina in cBot according to standard protocol Illumina USA.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP125291", null, "loader:fastq load.py", "Zf_heart_R1.fastq Zf_heart_R2.fastq", "fastq fastq", 5118546291.0, 51531220.0, "Zf heart R2.fastq", "0:99.33", "A:1442538320;C:1100438227;G:1124766301;T:1450751919;N:51524", 99, null, null, null, 1442538320, 1100438227, 1124766301, 1450751919, 51524, "SRX3408681", "SRS2701477", "SRA631861", "CSIR-Institute of Genomics and Integrative Biology|Genomic Medicine", "CSIR-Institute of Genomics and Integrative Biology", 1, 0.84707, null, 0.40863, null, 0.69037, null, 0.43672, null, 101, null, "B", null, "usable mapping rate", "illumina", "hiseq_era", "unknown", "random_priming", "ribozero", "bulk", "unknown", "unknown", null, "India", "2017-11-22", "Adult", "Adult", "Heart", "Cardiovascular System"], [44934, "SRR6308292", "SRX3408680", "SRS2701476", "SRP125291", "PRJNA419036", "Danio rerio strain:ASWT Transcriptome or Gene expression", "PRJNA419036", "Other", "A genome wide map of circular RNA in adult zebrafish.", null, null, null, "Zf brain", "Zf brain", null, "strain:ASWT|age:1year|sex:male|tissue:Brain|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "A genome wide map of circular RNA in adult zebrafish", "Zf brain", "Zf brain", "Tissue were isolated from adult zebrafish anaesthetized using 0.004% Tricaine Sigma USA. Extreme care was taken to avoid contamination to obtain pure homogenous tissue samples. The tissues were repeatedly washed in PBS to remove contaminating debris. The tissue samples were homogenized in Trizol for cell lysis Invitrogen USA. RNA was isolated from the homogenized tissue samples using RNeasy kit Qiagen USA. Sample preparation for sequencing was carried out using Truseq stranded RNA sample preparation kit Illumina USA as per supplier's instructions. In order to remove the ribosomal RNA rRNA one microgram of total RNA was hybridised with Ribo zero gold rRNA removal probe. Upon removing rRNA the samples were processed for fragmentation in the presence of ionic cations at 37 degree Celcius. First stranded complementary DNA cDNA was prepared by random hexamers and superscript II reverse transcriptase Invitrogen USA in presence of Actinomycin D to facilitate RNA dependent synthesis for improving strand specificity. The second strand was synthesised with second strand cDNA mix containing dUTP instead of dTTP and subjected to A base addition followed by adapter ligation. Final libraries were prepared by amplifying adapter ligated double strand cDNA. Clusters were generated on Hiseq flow cell v3 Illumina in cBot according to standard protocol Illumina USA.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP125291", null, null, "Zf_brain_R1.fastq Zf_brain_R2.fastq", "fastq fastq", 3103065297.0, 16700545.0, "Zf brain R2.fastq", "0:97.29 1:88.52", "A:832854751;C:721391526;G:701229714;T:847395346;N:193960", 97, 88, null, null, 832854751, 721391526, 701229714, 847395346, 193960, "SRX3408680", "SRS2701476", "SRA631861", "CSIR-Institute of Genomics and Integrative Biology|Genomic Medicine", "CSIR-Institute of Genomics and Integrative Biology", 2, 0.91265, 0.91134, 0.39205, 0.38961, 0.7778, 0.78094, 0.69798, 0.67369, 90, 45, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "ribozero", "bulk", "unknown", "unknown", null, "India", "2017-11-20", "Adult", "Adult", "Brain", "Nervous System"], [44935, "SRR6308293", "SRX3408679", "SRS2701475", "SRP125291", "PRJNA419036", "Danio rerio strain:ASWT Transcriptome or Gene expression", "PRJNA419036", "Other", "A genome wide map of circular RNA in adult zebrafish.", null, null, null, "Zf blood", "Zf blood", null, "strain:ASWT|age:1year|sex:male|tissue:Blood|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "A genome wide map of circular RNA in adult zebrafish", "Zf blood", "Zf blood", "Tissue were isolated from adult zebrafish anaesthetized using 0.004% Tricaine Sigma USA. Extreme care was taken to avoid contamination to obtain pure homogenous tissue samples. The tissues were repeatedly washed in PBS to remove contaminating debris. The tissue samples were homogenized in Trizol for cell lysis Invitrogen USA. RNA was isolated from the homogenized tissue samples using RNeasy kit Qiagen USA. Sample preparation for sequencing was carried out using Truseq stranded RNA sample preparation kit Illumina USA as per supplier's instructions. In order to remove the ribosomal RNA rRNA one microgram of total RNA was hybridised with Ribo zero gold rRNA removal probe. Upon removing rRNA the samples were processed for fragmentation in the presence of ionic cations at 37 degree Celcius. First stranded complementary DNA cDNA was prepared by random hexamers and superscript II reverse transcriptase Invitrogen USA in presence of Actinomycin D to facilitate RNA dependent synthesis for improving strand specificity. The second strand was synthesised with second strand cDNA mix containing dUTP instead of dTTP and subjected to A base addition followed by adapter ligation. Final libraries were prepared by amplifying adapter ligated double strand cDNA. Clusters were generated on Hiseq flow cell v3 Illumina in cBot according to standard protocol Illumina USA.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP125291", null, null, "Zf_blood_R1.fastq Zf_blood_R2.fastq", "fastq fastq", 3815054252.0, 20461146.0, "Zf blood R1.fastq", "0:97.58 1:88.87", "A:1003195592;C:903122443;G:887002461;T:1021496839;N:236917", 97, 88, null, null, 1003195592, 903122443, 887002461, 1021496839, 236917, "SRX3408679", "SRS2701475", "SRA631861", "CSIR-Institute of Genomics and Integrative Biology|Genomic Medicine", "CSIR-Institute of Genomics and Integrative Biology", 2, 0.88344, 0.89187, 0.31874, 0.32265, 0.75398, 0.75706, 0.6181, 0.61725, 100, 54, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "ribozero", "bulk", "unknown", "unknown", null, "India", "2017-11-20", "Adult", "Adult", "Blood", "Hematopoietic System"], [44936, "SRR6308294", "SRX3408678", "SRS2701474", "SRP125291", "PRJNA419036", "Danio rerio strain:ASWT Transcriptome or Gene expression", "PRJNA419036", "Other", "A genome wide map of circular RNA in adult zebrafish.", null, null, null, "Zf muscle", "Zf muscle", null, "strain:ASWT|age:1year|sex:male|tissue:Muscle|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "A genome wide map of circular RNA in adult zebrafish", "Zf muscle", "Zf muscle", "Tissue were isolated from adult zebrafish anaesthetized using 0.004% Tricaine Sigma USA. Extreme care was taken to avoid contamination to obtain pure homogenous tissue samples. The tissues were repeatedly washed in PBS to remove contaminating debris. The tissue samples were homogenized in Trizol for cell lysis Invitrogen USA. RNA was isolated from the homogenized tissue samples using RNeasy kit Qiagen USA. Sample preparation for sequencing was carried out using Truseq stranded RNA sample preparation kit Illumina USA as per supplier's instructions. In order to remove the ribosomal RNA rRNA one microgram of total RNA was hybridised with Ribo zero gold rRNA removal probe. Upon removing rRNA the samples were processed for fragmentation in the presence of ionic cations at 37 degree Celcius. First stranded complementary DNA cDNA was prepared by random hexamers and superscript II reverse transcriptase Invitrogen USA in presence of Actinomycin D to facilitate RNA dependent synthesis for improving strand specificity. The second strand was synthesised with second strand cDNA mix containing dUTP instead of dTTP and subjected to A base addition followed by adapter ligation. Final libraries were prepared by amplifying adapter ligated double strand cDNA. Clusters were generated on Hiseq flow cell v3 Illumina in cBot according to standard protocol Illumina USA.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP125291", null, null, "Zf_muscle_R1.fastq Zf_muscle_R2.fastq", "fastq fastq", 3777044386.0, 20362229.0, "Zf muscle R1.fastq", "0:97.14 1:88.35", "A:948171837;C:941487080;G:936414216;T:950742547;N:228706", 97, 88, null, null, 948171837, 941487080, 936414216, 950742547, 228706, "SRX3408678", "SRS2701474", "SRA631861", "CSIR-Institute of Genomics and Integrative Biology|Genomic Medicine", "CSIR-Institute of Genomics and Integrative Biology", 2, 0.94043, 0.955, 0.22684, 0.2231, 0.77222, 0.77368, 0.63355, 0.63196, 101, 93, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "ribozero", "bulk", "unknown", "unknown", null, "India", "2017-11-20", "Adult", "Adult", "Muscle", "Muscular System"], [48033, "SRR6921815", "SRX3869077", "SRS3112052", "SRP136856", "PRJNA448207", "Transcriptomic profiles of zebrafish liver exposed to phenanthrene", "PRJNA448207", "Other", "The present study aims to examine the toxicological effects of phenanthrene in the molecular mechanism in zebrafish. We performed RNA Seq analysis on zebrafish liver responding to phenanthrene exposure. Genes involved in phenanthrene exposure to zebrafish liver were identified from the transcriptional data. This study provides the completed evaluation of toxic effect of phenanthrene exposure to zebrafish by transcriptional approaches. Our findings could present foundation for further study on molecular mechanism responsible for zebrafish's responses to phenanthrene exposure.", null, null, "Fish in trplicate were anaesthetized with MS 222 at the dose of 10 mg/L and livers were sampled and pooled", null, "PHE3d.3", null, "strain:not applicable|isolate:not applicable|breed:AB line|cultivar:not applicable|ecotype:not applicable|age:3 month|dev stage:Adult fish|sex:pooled male and female|tissue:liver|biomaterial provider:China Zebrafish Resource Center|birth location:Wuhan China|breeding method:maintained in the semi static system under conditions of water quality and illumination as follows: temperature 2327 pH 78 dissolved oxygen concentration 5 mg L 1 total hardness 10250 mg CaCO3 per liter and light/dark period of 12:12 h.|genotype:AB line|health state:Healthy|sample type:tissue sample|treatment:Fish exposed to phenanthrene for xxx day at concentration of 300 g L 1|replicate:biological replicate 3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio: adult male and female exposed to phenanthrene for xxx day", "PHE3d.3", "PHE3d.3", "Random PCR", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "SRP136856", null, null, "PHE3d_3_1.fq.gz PHE3d_3_2.fq.gz", "fastq fastq", 6871439400.0, 22904798.0, "PHE3d 3 1.fq.gz", "0:150 1:150", "A:1771640937;C:1661273097;G:1659191846;T:1774900542;N:4432978", 150, 150, null, null, 1771640937, 1661273097, 1659191846, 1774900542, 4432978, "SRX3869077", "SRS3112052", "SRA678027", "Pearl River Fisheries Research Institute, CAFS|Fisheries environmental protection laboratory", "Pearl River Fisheries Research Institute, CAFS", 2, 0.92605, 0.9282, 0.04717, 0.04722, 0.72606, 0.73421, 0.51267, 0.50362, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2018-03-31", "Adult", "Adult", "Liver", "Liver and Biliary System"], [48034, "SRR6921816", "SRX3869076", "SRS3112051", "SRP136856", "PRJNA448207", "Transcriptomic profiles of zebrafish liver exposed to phenanthrene", "PRJNA448207", "Other", "The present study aims to examine the toxicological effects of phenanthrene in the molecular mechanism in zebrafish. We performed RNA Seq analysis on zebrafish liver responding to phenanthrene exposure. Genes involved in phenanthrene exposure to zebrafish liver were identified from the transcriptional data. This study provides the completed evaluation of toxic effect of phenanthrene exposure to zebrafish by transcriptional approaches. Our findings could present foundation for further study on molecular mechanism responsible for zebrafish's responses to phenanthrene exposure.", null, null, "Fish in trplicate were anaesthetized with MS 222 at the dose of 10 mg/L and livers were sampled and pooled", null, "PHE7d.1", null, "strain:not applicable|isolate:not applicable|breed:AB line|cultivar:not applicable|ecotype:not applicable|age:3 month|dev stage:Adult fish|sex:pooled male and female|tissue:liver|biomaterial provider:China Zebrafish Resource Center|birth location:Wuhan China|breeding method:maintained in the semi static system under conditions of water quality and illumination as follows: temperature 2327 pH 78 dissolved oxygen concentration 5 mg L 1 total hardness 10250 mg CaCO3 per liter and light/dark period of 12:12 h.|genotype:AB line|health state:Healthy|sample type:tissue sample|treatment:Fish exposed to phenanthrene for xxx day at concentration of 300 g L 1|replicate:biological replicate 1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio: adult male and female exposed to phenanthrene for xxx day", "PHE7d.1", "PHE7d.1", "Random PCR", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "SRP136856", null, null, "PHE7d_1_2.fq.gz PHE7d_1_1.fq.gz", "fastq fastq", 7415333700.0, 24717779.0, "PHE7d 1 2.fq.gz", "0:150 1:150", "A:1926590053;C:1783903190;G:1780648419;T:1919390554;N:4801484", 150, 150, null, null, 1926590053, 1783903190, 1780648419, 1919390554, 4801484, "SRX3869076", "SRS3112051", "SRA678027", "Pearl River Fisheries Research Institute, CAFS|Fisheries environmental protection laboratory", "Pearl River Fisheries Research Institute, CAFS", 2, 0.94375, 0.94379, 0.03337, 0.03376, 0.77567, 0.78589, 0.36225, 0.3605, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2018-03-31", "Adult", "Adult", "Liver", "Liver and Biliary System"], [48035, "SRR6921817", "SRX3869075", "SRS3112050", "SRP136856", "PRJNA448207", "Transcriptomic profiles of zebrafish liver exposed to phenanthrene", "PRJNA448207", "Other", "The present study aims to examine the toxicological effects of phenanthrene in the molecular mechanism in zebrafish. We performed RNA Seq analysis on zebrafish liver responding to phenanthrene exposure. Genes involved in phenanthrene exposure to zebrafish liver were identified from the transcriptional data. This study provides the completed evaluation of toxic effect of phenanthrene exposure to zebrafish by transcriptional approaches. 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We performed RNA Seq analysis on zebrafish liver responding to phenanthrene exposure. Genes involved in phenanthrene exposure to zebrafish liver were identified from the transcriptional data. This study provides the completed evaluation of toxic effect of phenanthrene exposure to zebrafish by transcriptional approaches. 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We performed RNA Seq analysis on zebrafish liver responding to phenanthrene exposure. Genes involved in phenanthrene exposure to zebrafish liver were identified from the transcriptional data. This study provides the completed evaluation of toxic effect of phenanthrene exposure to zebrafish by transcriptional approaches. 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We performed RNA Seq analysis on zebrafish liver responding to phenanthrene exposure. Genes involved in phenanthrene exposure to zebrafish liver were identified from the transcriptional data. This study provides the completed evaluation of toxic effect of phenanthrene exposure to zebrafish by transcriptional approaches. Our findings could present foundation for further study on molecular mechanism responsible for zebrafish's responses to phenanthrene exposure.", null, null, "Fish in trplicate were anaesthetized with MS 222 at the dose of 10 mg/L and livers were sampled and pooled", null, "PHE3d.2", null, "strain:not applicable|isolate:not applicable|breed:AB line|cultivar:not applicable|ecotype:not applicable|age:3 month|dev stage:Adult fish|sex:pooled male and female|tissue:liver|biomaterial provider:China Zebrafish Resource Center|birth location:Wuhan China|breeding method:maintained in the semi static system under conditions of water quality and illumination as follows: temperature 2327 pH 78 dissolved oxygen concentration 5 mg L 1 total hardness 10250 mg CaCO3 per liter and light/dark period of 12:12 h.|genotype:AB line|health state:Healthy|sample type:tissue sample|treatment:Fish exposed to phenanthrene for xxx day at concentration of 300 g L 1|replicate:biological replicate 2|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio: adult male and female exposed to phenanthrene for xxx day", "PHE3d.2", "PHE3d.2", "Random PCR", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "SRP136856", null, null, "PHE3d_2_1.fq.gz PHE3d_2_2.fq.gz", "fastq fastq", 8406789300.0, 28022631.0, "PHE3d 2 1.fq.gz", "0:150 1:150", "A:2187414004;C:2019038004;G:2013512837;T:2181333490;N:5490965", 150, 150, null, null, 2187414004, 2019038004, 2013512837, 2181333490, 5490965, "SRX3869072", "SRS3112047", "SRA678027", "Pearl River Fisheries Research Institute, CAFS|Fisheries environmental protection laboratory", "Pearl River Fisheries Research Institute, CAFS", 2, 0.91151, 0.91449, 0.01671, 0.017, 0.75315, 0.76203, 0.46837, 0.47419, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2018-03-31", "Adult", "Adult", "Liver", "Liver and Biliary System"], [48039, "SRR6921821", "SRX3869071", "SRS3112046", "SRP136856", "PRJNA448207", "Transcriptomic profiles of zebrafish liver exposed to phenanthrene", "PRJNA448207", "Other", "The present study aims to examine the toxicological effects of phenanthrene in the molecular mechanism in zebrafish. We performed RNA Seq analysis on zebrafish liver responding to phenanthrene exposure. Genes involved in phenanthrene exposure to zebrafish liver were identified from the transcriptional data. This study provides the completed evaluation of toxic effect of phenanthrene exposure to zebrafish by transcriptional approaches. Our findings could present foundation for further study on molecular mechanism responsible for zebrafish's responses to phenanthrene exposure.", null, null, "Fish in trplicate were anaesthetized with MS 222 at the dose of 10 mg/L and livers were sampled and pooled", null, "PHE0d.1", null, "strain:not applicable|isolate:not applicable|breed:AB line|cultivar:not applicable|ecotype:not applicable|age:3 month|dev stage:Adult fish|sex:pooled male and female|tissue:liver|biomaterial provider:China Zebrafish Resource Center|birth location:Wuhan China|breeding method:maintained in the semi static system under conditions of water quality and illumination as follows: temperature 2327 pH 78 dissolved oxygen concentration 5 mg L 1 total hardness 10250 mg CaCO3 per liter and light/dark period of 12:12 h.|genotype:AB line|health state:Healthy|sample type:tissue sample|treatment:Fish maintained without xxx exposure|replicate:biological replicate 1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio: adult male and female without xxx exposure", "PHE0d.1", "PHE0d.1", "Random PCR", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "SRP136856", null, null, "PHE0d_1_2.fq.gz PHE0d_1_1.fq.gz", "fastq fastq", 7853933100.0, 26179777.0, "PHE0d 1 2.fq.gz", "0:150 1:150", "A:2041706651;C:1888763231;G:1884281701;T:2035607198;N:3574319", 150, 150, null, null, 2041706651, 1888763231, 1884281701, 2035607198, 3574319, "SRX3869071", "SRS3112046", "SRA678027", "Pearl River Fisheries Research Institute, CAFS|Fisheries environmental protection laboratory", "Pearl River Fisheries Research Institute, CAFS", 2, 0.94164, 0.94393, 0.02233, 0.0224, 0.76625, 0.77356, 0.30965, 0.2981, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2018-03-31", "Adult", "Adult", "Liver", "Liver and Biliary System"], [48040, "SRR6921822", "SRX3869070", "SRS3112045", "SRP136856", "PRJNA448207", "Transcriptomic profiles of zebrafish liver exposed to phenanthrene", "PRJNA448207", "Other", "The present study aims to examine the toxicological effects of phenanthrene in the molecular mechanism in zebrafish. We performed RNA Seq analysis on zebrafish liver responding to phenanthrene exposure. Genes involved in phenanthrene exposure to zebrafish liver were identified from the transcriptional data. This study provides the completed evaluation of toxic effect of phenanthrene exposure to zebrafish by transcriptional approaches. Our findings could present foundation for further study on molecular mechanism responsible for zebrafish's responses to phenanthrene exposure.", null, null, "Fish in trplicate were anaesthetized with MS 222 at the dose of 10 mg/L and livers were sampled and pooled", null, "PHE0d.2", null, "strain:not applicable|isolate:not applicable|breed:AB line|cultivar:not applicable|ecotype:not applicable|age:3 month|dev stage:Adult fish|sex:pooled male and female|tissue:liver|biomaterial provider:China Zebrafish Resource Center|birth location:Wuhan China|breeding method:maintained in the semi static system under conditions of water quality and illumination as follows: temperature 2327 pH 78 dissolved oxygen concentration 5 mg L 1 total hardness 10250 mg CaCO3 per liter and light/dark period of 12:12 h.|genotype:AB line|health state:Healthy|sample type:tissue sample|treatment:Fish maintained without xxx exposure|replicate:biological replicate 2|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio: adult male and female without xxx exposure", "PHE0d.2", "PHE0d.2", "Random PCR", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "SRP136856", null, null, "PHE0d_2_2.fq.gz", "fastq", 3627859350.0, 24185729.0, "PHE0d 2 2.fq.gz", null, null, null, null, null, null, null, null, null, null, null, "SRX3869070", "SRS3112045", "SRA678027", "Pearl River Fisheries Research Institute, CAFS|Fisheries environmental protection laboratory", "Pearl River Fisheries Research Institute, CAFS", 1, 0.87829, null, 0.02367, null, 0.8213, null, 0.29999, null, 150, null, "B", null, "usable mapping rate", "illumina", "hiseq_era", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2018-03-31", "Adult", "Adult", "Liver", "Liver and Biliary System"], [48041, "SRR6921823", "SRX3869069", "SRS3112044", "SRP136856", "PRJNA448207", "Transcriptomic profiles of zebrafish liver exposed to phenanthrene", "PRJNA448207", "Other", "The present study aims to examine the toxicological effects of phenanthrene in the molecular mechanism in zebrafish. We performed RNA Seq analysis on zebrafish liver responding to phenanthrene exposure. Genes involved in phenanthrene exposure to zebrafish liver were identified from the transcriptional data. This study provides the completed evaluation of toxic effect of phenanthrene exposure to zebrafish by transcriptional approaches. Our findings could present foundation for further study on molecular mechanism responsible for zebrafish's responses to phenanthrene exposure.", null, null, "Fish in trplicate were anaesthetized with MS 222 at the dose of 10 mg/L and livers were sampled and pooled", null, "PHE0d.3", null, "strain:not applicable|isolate:not applicable|breed:AB line|cultivar:not applicable|ecotype:not applicable|age:3 month|dev stage:Adult fish|sex:pooled male and female|tissue:liver|biomaterial provider:China Zebrafish Resource Center|birth location:Wuhan China|breeding method:maintained in the semi static system under conditions of water quality and illumination as follows: temperature 2327 pH 78 dissolved oxygen concentration 5 mg L 1 total hardness 10250 mg CaCO3 per liter and light/dark period of 12:12 h.|genotype:AB line|health state:Healthy|sample type:tissue sample|treatment:Fish maintained without xxx exposure|replicate:biological replicate 3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio: adult male and female without xxx exposure", "PHE0d.3", "PHE0d.3", "Random PCR", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "SRP136856", null, null, "PHE0d_3_2.fq.gz", "fastq", 3650312550.0, 24335417.0, "PHE0d 3 2.fq.gz", "0:0 1:150", "A:945286124;C:876624311;G:880379003;T:946003595;N:2019517", 0, 150, null, null, 945286124, 876624311, 880379003, 946003595, 2019517, "SRX3869069", "SRS3112044", "SRA678027", "Pearl River Fisheries Research Institute, CAFS|Fisheries environmental protection laboratory", "Pearl River Fisheries Research Institute, CAFS", 1, 0.93747, null, 0.0274, null, 0.76031, null, 0.35099, null, 150, null, "B", null, "usable mapping rate", "illumina", "hiseq_era", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2018-03-31", "Adult", "Adult", "Liver", "Liver and Biliary System"], [48042, "SRR6921824", "SRX3869068", "SRS3112043", "SRP136856", "PRJNA448207", "Transcriptomic profiles of zebrafish liver exposed to phenanthrene", "PRJNA448207", "Other", "The present study aims to examine the toxicological effects of phenanthrene in the molecular mechanism in zebrafish. We performed RNA Seq analysis on zebrafish liver responding to phenanthrene exposure. Genes involved in phenanthrene exposure to zebrafish liver were identified from the transcriptional data. This study provides the completed evaluation of toxic effect of phenanthrene exposure to zebrafish by transcriptional approaches. Our findings could present foundation for further study on molecular mechanism responsible for zebrafish's responses to phenanthrene exposure.", null, null, "Fish in trplicate were anaesthetized with MS 222 at the dose of 10 mg/L and livers were sampled and pooled", null, "PHE1d.1", null, "strain:not applicable|isolate:not applicable|breed:AB line|cultivar:not applicable|ecotype:not applicable|age:3 month|dev stage:Adult fish|sex:pooled male and female|tissue:liver|biomaterial provider:China Zebrafish Resource Center|birth location:Wuhan China|breeding method:maintained in the semi static system under conditions of water quality and illumination as follows: temperature 2327 pH 78 dissolved oxygen concentration 5 mg L 1 total hardness 10250 mg CaCO3 per liter and light/dark period of 12:12 h.|genotype:AB line|health state:Healthy|sample type:tissue sample|treatment:Fish exposed to phenanthrene for xxx day at concentration of 300 g L 1|replicate:biological replicate 1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio: adult male and female exposed to phenanthrene for xxx day", "PHE1d.1", "PHE1d.1", "Random PCR", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "SRP136856", null, null, "PHE1d_1_1.fq.gz PHE1d_1_2.fq.gz", "fastq fastq", 7353145800.0, 24510486.0, "PHE1d 1 1.fq.gz", "0:150 1:150", "A:1896735212;C:1783435525;G:1779850974;T:1888352720;N:4771369", 150, 150, null, null, 1896735212, 1783435525, 1779850974, 1888352720, 4771369, "SRX3869068", "SRS3112043", "SRA678027", "Pearl River Fisheries Research Institute, CAFS|Fisheries environmental protection laboratory", "Pearl River Fisheries Research Institute, CAFS", 2, 0.9244, 0.92601, 0.01781, 0.01775, 0.74884, 0.75507, 0.43251, 0.44499, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2018-03-31", "Adult", "Adult", "Liver", "Liver and Biliary System"], [48043, "SRR6921825", "SRX3869067", "SRS3112042", "SRP136856", "PRJNA448207", "Transcriptomic profiles of zebrafish liver exposed to phenanthrene", "PRJNA448207", "Other", "The present study aims to examine the toxicological effects of phenanthrene in the molecular mechanism in zebrafish. We performed RNA Seq analysis on zebrafish liver responding to phenanthrene exposure. Genes involved in phenanthrene exposure to zebrafish liver were identified from the transcriptional data. This study provides the completed evaluation of toxic effect of phenanthrene exposure to zebrafish by transcriptional approaches. Our findings could present foundation for further study on molecular mechanism responsible for zebrafish's responses to phenanthrene exposure.", null, null, "Fish in trplicate were anaesthetized with MS 222 at the dose of 10 mg/L and livers were sampled and pooled", null, "PHE7d.2", null, "strain:not applicable|isolate:not applicable|breed:AB line|cultivar:not applicable|ecotype:not applicable|age:3 month|dev stage:Adult fish|sex:pooled male and female|tissue:liver|biomaterial provider:China Zebrafish Resource Center|birth location:Wuhan China|breeding method:maintained in the semi static system under conditions of water quality and illumination as follows: temperature 2327 pH 78 dissolved oxygen concentration 5 mg L 1 total hardness 10250 mg CaCO3 per liter and light/dark period of 12:12 h.|genotype:AB line|health state:Healthy|sample type:tissue sample|treatment:Fish exposed to phenanthrene for xxx day at concentration of 300 g L 1|replicate:biological replicate 2|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio: adult male and female exposed to phenanthrene for xxx day", "PHE7d.2", "PHE7d.2", "Random PCR", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "SRP136856", null, null, "PHE7d_2_1.fq.gz PHE7d_2_2.fq.gz", "fastq fastq", 7224224100.0, 24080747.0, "PHE7d 2 1.fq.gz", "0:150 1:150", "A:1825676690;C:1788393328;G:1782936749;T:1822524668;N:4692665", 150, 150, null, null, 1825676690, 1788393328, 1782936749, 1822524668, 4692665, "SRX3869067", "SRS3112042", "SRA678027", "Pearl River Fisheries Research Institute, CAFS|Fisheries environmental protection laboratory", "Pearl River Fisheries Research Institute, CAFS", 2, 0.9373, 0.9391, 0.05048, 0.05061, 0.75939, 0.76917, 0.47799, 0.50408, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2018-03-31", "Adult", "Adult", "Liver", "Liver and Biliary System"], [48044, "SRR6921826", "SRX3869066", "SRS3112041", "SRP136856", "PRJNA448207", "Transcriptomic profiles of zebrafish liver exposed to phenanthrene", "PRJNA448207", "Other", "The present study aims to examine the toxicological effects of phenanthrene in the molecular mechanism in zebrafish. We performed RNA Seq analysis on zebrafish liver responding to phenanthrene exposure. Genes involved in phenanthrene exposure to zebrafish liver were identified from the transcriptional data. This study provides the completed evaluation of toxic effect of phenanthrene exposure to zebrafish by transcriptional approaches. Our findings could present foundation for further study on molecular mechanism responsible for zebrafish's responses to phenanthrene exposure.", null, null, "Fish in trplicate were anaesthetized with MS 222 at the dose of 10 mg/L and livers were sampled and pooled", null, "PHE7d.3", null, "strain:not applicable|isolate:not applicable|breed:AB line|cultivar:not applicable|ecotype:not applicable|age:3 month|dev stage:Adult fish|sex:pooled male and female|tissue:liver|biomaterial provider:China Zebrafish Resource Center|birth location:Wuhan China|breeding method:maintained in the semi static system under conditions of water quality and illumination as follows: temperature 2327 pH 78 dissolved oxygen concentration 5 mg L 1 total hardness 10250 mg CaCO3 per liter and light/dark period of 12:12 h.|genotype:AB line|health state:Healthy|sample type:tissue sample|treatment:Fish exposed to phenanthrene for xxx day at concentration of 300 g L 1|replicate:biological replicate 3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio: adult male and female exposed to phenanthrene for xxx day", "PHE7d.3", "PHE7d.3", "Random PCR", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "SRP136856", null, null, "PHE7d_3_1.fq.gz PHE7d_3_2.fq.gz", "fastq fastq", 7251597900.0, 24171993.0, "PHE7d 3 1.fq.gz", "0:150 1:150", "A:1868321462;C:1755689716;G:1756761060;T:1866130827;N:4694835", 150, 150, null, null, 1868321462, 1755689716, 1756761060, 1866130827, 4694835, "SRX3869066", "SRS3112041", "SRA678027", "Pearl River Fisheries Research Institute, CAFS|Fisheries environmental protection laboratory", "Pearl River Fisheries Research Institute, CAFS", 2, 0.92489, 0.9282, 0.04608, 0.04554, 0.74961, 0.75814, 0.52555, 0.53342, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2018-03-31", "Adult", "Adult", "Liver", "Liver and Biliary System"], [48045, "SRR6921827", "SRX3869065", "SRS3112040", "SRP136856", "PRJNA448207", "Transcriptomic profiles of zebrafish liver exposed to phenanthrene", "PRJNA448207", "Other", "The present study aims to examine the toxicological effects of phenanthrene in the molecular mechanism in zebrafish. We performed RNA Seq analysis on zebrafish liver responding to phenanthrene exposure. Genes involved in phenanthrene exposure to zebrafish liver were identified from the transcriptional data. This study provides the completed evaluation of toxic effect of phenanthrene exposure to zebrafish by transcriptional approaches. Our findings could present foundation for further study on molecular mechanism responsible for zebrafish's responses to phenanthrene exposure.", null, null, "Fish in trplicate were anaesthetized with MS 222 at the dose of 10 mg/L and livers were sampled and pooled", null, "PHE15d.1", null, "strain:not applicable|isolate:not applicable|breed:AB line|cultivar:not applicable|ecotype:not applicable|age:3 month|dev stage:Adult fish|sex:pooled male and female|tissue:liver|biomaterial provider:China Zebrafish Resource Center|birth location:Wuhan China|breeding method:maintained in the semi static system under conditions of water quality and illumination as follows: temperature 2327 pH 78 dissolved oxygen concentration 5 mg L 1 total hardness 10250 mg CaCO3 per liter and light/dark period of 12:12 h.|genotype:AB line|health state:Healthy|sample type:tissue sample|treatment:Fish exposed to phenanthrene for xxx day at concentration of 300 g L 1|replicate:biological replicate 1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio: adult male and female exposed to phenanthrene for xxx day", "PHE15d.1", "PHE15d.1", "Random PCR", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "SRP136856", null, null, "PHE15d_1_1.fq.gz PHE15d_1_2.fq.gz", "fastq fastq", 8022302100.0, 26741007.0, "PHE15d 1 1.fq.gz", "0:150 1:150", "A:2050760820;C:1951106961;G:1960909235;T:2054339788;N:5185296", 150, 150, null, null, 2050760820, 1951106961, 1960909235, 2054339788, 5185296, "SRX3869065", "SRS3112040", "SRA678027", "Pearl River Fisheries Research Institute, CAFS|Fisheries environmental protection laboratory", "Pearl River Fisheries Research Institute, CAFS", 2, 0.93832, 0.94188, 0.03193, 0.03228, 0.77828, 0.78632, 0.5109, 0.50873, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2018-03-31", "Adult", "Adult", "Liver", "Liver and Biliary System"], [48046, "SRR6921828", "SRX3869064", "SRS3112039", "SRP136856", "PRJNA448207", "Transcriptomic profiles of zebrafish liver exposed to phenanthrene", "PRJNA448207", "Other", "The present study aims to examine the toxicological effects of phenanthrene in the molecular mechanism in zebrafish. We performed RNA Seq analysis on zebrafish liver responding to phenanthrene exposure. Genes involved in phenanthrene exposure to zebrafish liver were identified from the transcriptional data. This study provides the completed evaluation of toxic effect of phenanthrene exposure to zebrafish by transcriptional approaches. Our findings could present foundation for further study on molecular mechanism responsible for zebrafish's responses to phenanthrene exposure.", null, null, "Fish in trplicate were anaesthetized with MS 222 at the dose of 10 mg/L and livers were sampled and pooled", null, "PHE15d.2", null, "strain:not applicable|isolate:not applicable|breed:AB line|cultivar:not applicable|ecotype:not applicable|age:3 month|dev stage:Adult fish|sex:pooled male and female|tissue:liver|biomaterial provider:China Zebrafish Resource Center|birth location:Wuhan China|breeding method:maintained in the semi static system under conditions of water quality and illumination as follows: temperature 2327 pH 78 dissolved oxygen concentration 5 mg L 1 total hardness 10250 mg CaCO3 per liter and light/dark period of 12:12 h.|genotype:AB line|health state:Healthy|sample type:tissue sample|treatment:Fish exposed to phenanthrene for xxx day at concentration of 300 g L 1|replicate:biological replicate 2|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio: adult male and female exposed to phenanthrene for xxx day", "PHE15d.2", "PHE15d.2", "Random PCR", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "SRP136856", null, null, "PHE15d_2_1.fq.gz PHE15d_2_2.fq.gz", "fastq fastq", 8034285000.0, 26780950.0, "PHE15d 2 2.fq.gz", "0:150 1:150", "A:2073938434;C:1941404499;G:1938205281;T:2075525314;N:5211472", 150, 150, null, null, 2073938434, 1941404499, 1938205281, 2075525314, 5211472, "SRX3869064", "SRS3112039", "SRA678027", "Pearl River Fisheries Research Institute, CAFS|Fisheries environmental protection laboratory", "Pearl River Fisheries Research Institute, CAFS", 2, 0.93687, 0.93932, 0.0343, 0.03469, 0.74797, 0.75676, 0.46939, 0.48083, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2018-03-31", "Adult", "Adult", "Liver", "Liver and Biliary System"], [48047, "SRR6921829", "SRX3869063", "SRS3112038", "SRP136856", "PRJNA448207", "Transcriptomic profiles of zebrafish liver exposed to phenanthrene", "PRJNA448207", "Other", "The present study aims to examine the toxicological effects of phenanthrene in the molecular mechanism in zebrafish. We performed RNA Seq analysis on zebrafish liver responding to phenanthrene exposure. Genes involved in phenanthrene exposure to zebrafish liver were identified from the transcriptional data. This study provides the completed evaluation of toxic effect of phenanthrene exposure to zebrafish by transcriptional approaches. Our findings could present foundation for further study on molecular mechanism responsible for zebrafish's responses to phenanthrene exposure.", null, null, "Fish in trplicate were anaesthetized with MS 222 at the dose of 10 mg/L and livers were sampled and pooled", null, "PHE15d.3", null, "strain:not applicable|isolate:not applicable|breed:AB line|cultivar:not applicable|ecotype:not applicable|age:3 month|dev stage:Adult fish|sex:pooled male and female|tissue:liver|biomaterial provider:China Zebrafish Resource Center|birth location:Wuhan China|breeding method:maintained in the semi static system under conditions of water quality and illumination as follows: temperature 2327 pH 78 dissolved oxygen concentration 5 mg L 1 total hardness 10250 mg CaCO3 per liter and light/dark period of 12:12 h.|genotype:AB line|health state:Healthy|sample type:tissue sample|treatment:Fish exposed to phenanthrene for xxx day at concentration of 300 g L 1|replicate:biological replicate 3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio: adult male and female exposed to phenanthrene for xxx day", "PHE15d.3", "PHE15d.3", "Random PCR", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "SRP136856", null, null, "PHE15d_3_1.fq.gz PHE15d_3_2.fq.gz", "fastq fastq", 7768190100.0, 25893967.0, "PHE15d 3 2.fq.gz", "0:150 1:150", "A:2003796503;C:1879716153;G:1873169874;T:2006471079;N:5036491", 150, 150, null, null, 2003796503, 1879716153, 1873169874, 2006471079, 5036491, "SRX3869063", "SRS3112038", "SRA678027", "Pearl River Fisheries Research Institute, CAFS|Fisheries environmental protection laboratory", "Pearl River Fisheries Research Institute, CAFS", 2, 0.92663, 0.92862, 0.05515, 0.05537, 0.67915, 0.6886, 0.52556, 0.52171, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2018-03-31", "Adult", "Adult", "Liver", "Liver and Biliary System"], [49742, "SRR8197481", "SRX5016863", "SRS4049994", "SRP169068", "PRJNA495892", "Danio rerio Genome sequencing", "PRJNA495892", "Whole Genome Sequencing", "Transcriptome data of zebrafish in two stages of unfertilized egg and prim 5.", null, null, "three samples of zebrafish unfertilized eggs and three samples zebrafish fries at the stage of prim5.", "Model organism or animal sample from Danio rerio", "The transcriptome data of zebrafish in two stages of unfertilized egg and prim 5.", null, "isolate:zebrafish unfertilized eggs and zebrafish fries at the prim5 stage|dev stage:2 stages of unfertilized egg and prim 5|sex:pooled male and female|tissue:zebrafish in 2 stages of unfertilized egg and prim 5|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio: prim5 stage", "Z2 3 F", "Z2 3 F", "The unfertilized eggs of zebrafish were taken as three samples Z1 1 Z1 2 and Z1 3 respectively. And the fries of zebrafish were taken as three samples Z2 1 Z2 2 and Z2 3 in the prim5 stage.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP169068", null, null, "Z2_3_1.fq.gz Z2_3_2.fq.gz", "fastq fastq", 6979181500.0, 27916726.0, "Z2 3 1.fq.gz", "0:125 1:125", "A:1822429760;C:1670132153;G:1694226275;T:1791552753;N:840559", 125, 125, null, null, 1822429760, 1670132153, 1694226275, 1791552753, 840559, "SRX5016863", "SRS4049994", "SRA810793", "Institute of Hydrobiology, Chinese Academy of Sciences|State Key Laboratory of Freshwater Ecology and Bio", "Institute of Hydrobiology, Chinese Academy of Sciences", 2, 0.9586, 0.9518, 0.02217, 0.02129, 0.81022, 0.80921, 0.50342, 0.51319, 125, 125, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2018-11-16", "Multi-stage", "Embryo", "Oocyte", "Reproductive System"], [49743, "SRR8197482", "SRX5016862", "SRS4049994", "SRP169068", "PRJNA495892", "Danio rerio Genome sequencing", "PRJNA495892", "Whole Genome Sequencing", "Transcriptome data of zebrafish in two stages of unfertilized egg and prim 5.", null, null, "three samples of zebrafish unfertilized eggs and three samples zebrafish fries at the stage of prim5.", "Model organism or animal sample from Danio rerio", "The transcriptome data of zebrafish in two stages of unfertilized egg and prim 5.", null, "isolate:zebrafish unfertilized eggs and zebrafish fries at the prim5 stage|dev stage:2 stages of unfertilized egg and prim 5|sex:pooled male and female|tissue:zebrafish in 2 stages of unfertilized egg and prim 5|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio: prim5 stage", "Z2 1 F", "Z2 1 F", "The unfertilized eggs of zebrafish were taken as three samples Z1 1 Z1 2 and Z1 3 respectively. And the fries of zebrafish were taken as three samples Z2 1 Z2 2 and Z2 3 in the prim5 stage.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP169068", null, null, "Z2_1_2.fq.gz Z2_1_1.fq.gz", "fastq fastq", 6728695750.0, 26914783.0, "Z2 1 1.fq.gz", "0:125 1:125", "A:1732100690;C:1624557456;G:1649046037;T:1722193010;N:798557", 125, 125, null, null, 1732100690, 1624557456, 1649046037, 1722193010, 798557, "SRX5016862", "SRS4049994", "SRA810793", "Institute of Hydrobiology, Chinese Academy of Sciences|State Key Laboratory of Freshwater Ecology and Bio", "Institute of Hydrobiology, Chinese Academy of Sciences", 2, 0.9585, 0.95335, 0.01902, 0.01833, 0.8131, 0.81014, 0.48431, 0.493, 125, 125, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2018-11-16", "Multi-stage", "Embryo", "Oocyte", "Reproductive System"], [49744, "SRR8197483", "SRX5016861", "SRS4049994", "SRP169068", "PRJNA495892", "Danio rerio Genome sequencing", "PRJNA495892", "Whole Genome Sequencing", "Transcriptome data of zebrafish in two stages of unfertilized egg and prim 5.", null, null, "three samples of zebrafish unfertilized eggs and three samples zebrafish fries at the stage of prim5.", "Model organism or animal sample from Danio rerio", "The transcriptome data of zebrafish in two stages of unfertilized egg and prim 5.", null, "isolate:zebrafish unfertilized eggs and zebrafish fries at the prim5 stage|dev stage:2 stages of unfertilized egg and prim 5|sex:pooled male and female|tissue:zebrafish in 2 stages of unfertilized egg and prim 5|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio: unfertilized egg", "Z1 3 F", "Z1 3 F", "The unfertilized eggs of zebrafish were taken as three samples Z1 1 Z1 2 and Z1 3 respectively. And the fries of zebrafish were taken as three samples Z2 1 Z2 2 and Z2 3 in the prim5 stage.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP169068", null, null, "Z1_3_1.fq.gz Z1_3_2.fq.gz", "fastq fastq", 6657035500.0, 26628142.0, "Z1 3 1.fq.gz", "0:125 1:125", "A:1796541492;C:1535300915;G:1557971133;T:1766443902;N:778058", 125, 125, null, null, 1796541492, 1535300915, 1557971133, 1766443902, 778058, "SRX5016861", "SRS4049994", "SRA810793", "Institute of Hydrobiology, Chinese Academy of Sciences|State Key Laboratory of Freshwater Ecology and Bio", "Institute of Hydrobiology, Chinese Academy of Sciences", 2, 0.95543, 0.94989, 0.02312, 0.02201, 0.79689, 0.79421, 0.51212, 0.51203, 125, 125, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2018-11-16", "Multi-stage", "Embryo", "Oocyte", "Reproductive System"], [49745, "SRR8197484", "SRX5016860", "SRS4049994", "SRP169068", "PRJNA495892", "Danio rerio Genome sequencing", "PRJNA495892", "Whole Genome Sequencing", "Transcriptome data of zebrafish in two stages of unfertilized egg and prim 5.", null, null, "three samples of zebrafish unfertilized eggs and three samples zebrafish fries at the stage of prim5.", "Model organism or animal sample from Danio rerio", "The transcriptome data of zebrafish in two stages of unfertilized egg and prim 5.", null, "isolate:zebrafish unfertilized eggs and zebrafish fries at the prim5 stage|dev stage:2 stages of unfertilized egg and prim 5|sex:pooled male and female|tissue:zebrafish in 2 stages of unfertilized egg and prim 5|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio: unfertilized egg", "Z1 2 F", "Z1 2 F", "The unfertilized eggs of zebrafish were taken as three samples Z1 1 Z1 2 and Z1 3 respectively. And the fries of zebrafish were taken as three samples Z2 1 Z2 2 and Z2 3 in the prim5 stage.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP169068", null, null, "Z1_2_1.fq.gz Z1_2_2.fq.gz", "fastq fastq", 8869328500.0, 35477314.0, "Z1 2 1.fq.gz", "0:125 1:125", "A:2367358711;C:2085652811;G:2116692954;T:2298597848;N:1026176", 125, 125, null, null, 2367358711, 2085652811, 2116692954, 2298597848, 1026176, "SRX5016860", "SRS4049994", "SRA810793", "Institute of Hydrobiology, Chinese Academy of Sciences|State Key Laboratory of Freshwater Ecology and Bio", "Institute of Hydrobiology, Chinese Academy of Sciences", 2, 0.95776, 0.95084, 0.01896, 0.01811, 0.79823, 0.79608, 0.49565, 0.49345, 125, 125, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2018-11-18", "Multi-stage", "Embryo", "Oocyte", "Reproductive System"], [49746, "SRR8197485", "SRX5016859", "SRS4049994", "SRP169068", "PRJNA495892", "Danio rerio Genome sequencing", "PRJNA495892", "Whole Genome Sequencing", "Transcriptome data of zebrafish in two stages of unfertilized egg and prim 5.", null, null, "three samples of zebrafish unfertilized eggs and three samples zebrafish fries at the stage of prim5.", "Model organism or animal sample from Danio rerio", "The transcriptome data of zebrafish in two stages of unfertilized egg and prim 5.", null, "isolate:zebrafish unfertilized eggs and zebrafish fries at the prim5 stage|dev stage:2 stages of unfertilized egg and prim 5|sex:pooled male and female|tissue:zebrafish in 2 stages of unfertilized egg and prim 5|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio: unfertilized egg", "Z1 1 F", "Z1 1 F", "The unfertilized eggs of zebrafish were taken as three samples Z1 1 Z1 2 and Z1 3 respectively. And the fries of zebrafish were taken as three samples Z2 1 Z2 2 and Z2 3 in the prim5 stage.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP169068", null, null, "Z1_1_1.fq.gz Z1_1_2.fq.gz", "fastq fastq", 7205390500.0, 28821562.0, "Z1 1 1.fq.gz", "0:125 1:125", "A:1900562357;C:1700185416;G:1728383049;T:1875415232;N:844446", 125, 125, null, null, 1900562357, 1700185416, 1728383049, 1875415232, 844446, "SRX5016859", "SRS4049994", "SRA810793", "Institute of Hydrobiology, Chinese Academy of Sciences|State Key Laboratory of Freshwater Ecology and Bio", "Institute of Hydrobiology, Chinese Academy of Sciences", 2, 0.95593, 0.95003, 0.01844, 0.01778, 0.80064, 0.80008, 0.4858, 0.48331, 125, 125, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2018-11-18", "Multi-stage", "Embryo", "Oocyte", "Reproductive System"], [49747, "SRR8197486", "SRX5016858", "SRS4049994", "SRP169068", "PRJNA495892", "Danio rerio Genome sequencing", "PRJNA495892", "Whole Genome Sequencing", "Transcriptome data of zebrafish in two stages of unfertilized egg and prim 5.", null, null, "three samples of zebrafish unfertilized eggs and three samples zebrafish fries at the stage of prim5.", "Model organism or animal sample from Danio rerio", "The transcriptome data of zebrafish in two stages of unfertilized egg and prim 5.", null, "isolate:zebrafish unfertilized eggs and zebrafish fries at the prim5 stage|dev stage:2 stages of unfertilized egg and prim 5|sex:pooled male and female|tissue:zebrafish in 2 stages of unfertilized egg and prim 5|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio: prim5 stage", "Z2 2 F", "Z2 2 F", "The unfertilized eggs of zebrafish were taken as three samples Z1 1 Z1 2 and Z1 3 respectively. And the fries of zebrafish were taken as three samples Z2 1 Z2 2 and Z2 3 in the prim5 stage.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP169068", null, null, "Z2_2_2.fq.gz Z2_2_1.fq.gz", "fastq fastq", 7724266250.0, 30897065.0, "Z2 2 1.fq.gz", "0:125 1:125", "A:1986625683;C:1864133349;G:1894026843;T:1978566785;N:913590", 125, 125, null, null, 1986625683, 1864133349, 1894026843, 1978566785, 913590, "SRX5016858", "SRS4049994", "SRA810793", "Institute of Hydrobiology, Chinese Academy of Sciences|State Key Laboratory of Freshwater Ecology and Bio", "Institute of Hydrobiology, Chinese Academy of Sciences", 2, 0.95577, 0.94831, 0.01968, 0.01899, 0.80793, 0.80667, 0.48789, 0.48551, 125, 125, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2018-11-16", "Multi-stage", "Embryo", "Oocyte", "Reproductive System"], [50511, "SRR8293425", "SRX5108028", "SRS4117494", "SRP172911", "PRJNA498365", "Raw sequence reads in the unfertilized eggs of zebrafish Danio rerio", "PRJNA498365", "Other", "Raw sequence reads of full length transcriptome sequencing in the unfertilized eggs of zebrafish Danio rerio.", null, null, "Three female zebrafish were selected and 100 unfertilized eggs from each female were harvested as three samples Z1 1 Z1 2 and Z1 3 respectively; Identical quantities of RNA from Z1 1 Z1 2 and Z1 3 were combined into sample Z1 M.", "Model organism or animal sample from Danio rerio", "unfertilized eggs from three female zebrafish were harvested as samples Z1 M", null, "isolate:3 female zebrafish were selected and 100 unfertilized eggs from each female were harvested as 3 samples Z1 1 Z1 2 and Z1 3 respectively; and identical quantities of RNA from Z1 1 Z1 2 and Z1 3 were combined into sample Z1 M.|dev stage:unfertilized egg|sex:female|tissue:ovary|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Full length transcriptome sequencing of zebrafish Danio rerio : adult female unfertilized eggs.", "2 3K D03 1", "2 3K D03 1", "RNA sample Z1 M was reverse transcribed into cDNA. Four fragment ranges 0.5 1K 1 2K 2 3K and >3K were prepared according to the PacBio Iso Seq protocol. PacBio RS II sequencing reactions of 16 SMRT single molecule real time cells 3 cells of 0.5 1K 5 cells of 1 2K 5 cells of 2 3K 3 cells of >3K were performed in DNA Sequencing Reagent 4.0 Clontech Mountain View California USA.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "SINGLE", "PACBIO_SMRT", "PacBio RS II", null, "SRP172911", null, null, "m160828_125709_42199_c101055502550000001823247601061763_s1_p0.1.bax.h5 m160828_125709_42199_c101055502550000001823247601061763_s1_p0.2.bax.h5 m160828_125709_42199_c101055502550000001823247601061763_s1_p0.3.bax.h5 m160828_125709_42199_c101055502550000001823247601061763_s1_p0.bas.h5", "pacbio_native pacbio_native pacbio_native pacbio_native", 3189114337.0, 163482.0, "2 3K D03 1.tar.gz", null, "A:1019691460;C:775938787;G:628460211;T:736274173;N:0", null, null, null, null, 1019691460, 775938787, 628460211, 736274173, 0, "SRX5108028", "SRS4117494", "SRA820074", "Institute of Hydrobiology, Chinese Academy of Sciences|State Key Laboratory of Freshwater Ecology and Bio", "Institute of Hydrobiology, Chinese Academy of Sciences", null, null, null, null, null, null, null, null, null, null, null, null, null, null, "pacbio", "pacbio_early", "full_length", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2018-12-10", "Zygote", "Embryo", "Gonad", "Reproductive System"], [50512, "SRR8293426", "SRX5108027", "SRS4117494", "SRP172911", "PRJNA498365", "Raw sequence reads in the unfertilized eggs of zebrafish Danio rerio", "PRJNA498365", "Other", "Raw sequence reads of full length transcriptome sequencing in the unfertilized eggs of zebrafish Danio rerio.", null, null, "Three female zebrafish were selected and 100 unfertilized eggs from each female were harvested as three samples Z1 1 Z1 2 and Z1 3 respectively; Identical quantities of RNA from Z1 1 Z1 2 and Z1 3 were combined into sample Z1 M.", "Model organism or animal sample from Danio rerio", "unfertilized eggs from three female zebrafish were harvested as samples Z1 M", null, "isolate:3 female zebrafish were selected and 100 unfertilized eggs from each female were harvested as 3 samples Z1 1 Z1 2 and Z1 3 respectively; and identical quantities of RNA from Z1 1 Z1 2 and Z1 3 were combined into sample Z1 M.|dev stage:unfertilized egg|sex:female|tissue:ovary|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Full length transcriptome sequencing of zebrafish Danio rerio : adult female unfertilized eggs.", "2 3K E03 1", "2 3K E03 1", "RNA sample Z1 M was reverse transcribed into cDNA. Four fragment ranges 0.5 1K 1 2K 2 3K and >3K were prepared according to the PacBio Iso Seq protocol. PacBio RS II sequencing reactions of 16 SMRT single molecule real time cells 3 cells of 0.5 1K 5 cells of 1 2K 5 cells of 2 3K 3 cells of >3K were performed in DNA Sequencing Reagent 4.0 Clontech Mountain View California USA.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "SINGLE", "PACBIO_SMRT", "PacBio RS II", null, "SRP172911", null, null, "m160828_171546_42199_c101055502550000001823247601061764_s1_p0.1.bax.h5 m160828_171546_42199_c101055502550000001823247601061764_s1_p0.2.bax.h5 m160828_171546_42199_c101055502550000001823247601061764_s1_p0.3.bax.h5 m160828_171546_42199_c101055502550000001823247601061764_s1_p0.bas.h5", "pacbio_native pacbio_native pacbio_native pacbio_native", 3603145484.0, 163482.0, "2 3K E03 1.tar.gz", null, "A:1041332538;C:789941375;G:785608794;T:952634306;N:0", null, null, null, null, 1041332538, 789941375, 785608794, 952634306, 0, "SRX5108027", "SRS4117494", "SRA820074", "Institute of Hydrobiology, Chinese Academy of Sciences|State Key Laboratory of Freshwater Ecology and Bio", "Institute of Hydrobiology, Chinese Academy of Sciences", null, null, null, null, null, null, null, null, null, null, null, null, null, null, "pacbio", "pacbio_early", "full_length", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2018-12-10", "Zygote", "Embryo", "Gonad", "Reproductive System"], [50513, "SRR8293427", "SRX5108026", "SRS4117494", "SRP172911", "PRJNA498365", "Raw sequence reads in the unfertilized eggs of zebrafish Danio rerio", "PRJNA498365", "Other", "Raw sequence reads of full length transcriptome sequencing in the unfertilized eggs of zebrafish Danio rerio.", null, null, "Three female zebrafish were selected and 100 unfertilized eggs from each female were harvested as three samples Z1 1 Z1 2 and Z1 3 respectively; Identical quantities of RNA from Z1 1 Z1 2 and Z1 3 were combined into sample Z1 M.", "Model organism or animal sample from Danio rerio", "unfertilized eggs from three female zebrafish were harvested as samples Z1 M", null, "isolate:3 female zebrafish were selected and 100 unfertilized eggs from each female were harvested as 3 samples Z1 1 Z1 2 and Z1 3 respectively; and identical quantities of RNA from Z1 1 Z1 2 and Z1 3 were combined into sample Z1 M.|dev stage:unfertilized egg|sex:female|tissue:ovary|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Full length transcriptome sequencing of zebrafish Danio rerio : adult female unfertilized eggs.", "1 2K D05 1", "1 2K D05 1", "RNA sample Z1 M was reverse transcribed into cDNA. Four fragment ranges 0.5 1K 1 2K 2 3K and >3K were prepared according to the PacBio Iso Seq protocol. PacBio RS II sequencing reactions of 16 SMRT single molecule real time cells 3 cells of 0.5 1K 5 cells of 1 2K 5 cells of 2 3K 3 cells of >3K were performed in DNA Sequencing Reagent 4.0 Clontech Mountain View California USA.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "SINGLE", "PACBIO_SMRT", "PacBio RS II", null, "SRP172911", null, null, "m160831_064528_42199_c101016682550000001823228410211623_s1_p0.1.bax.h5 m160831_064528_42199_c101016682550000001823228410211623_s1_p0.2.bax.h5 m160831_064528_42199_c101016682550000001823228410211623_s1_p0.3.bax.h5 m160831_064528_42199_c101016682550000001823228410211623_s1_p0.bas.h5", "pacbio_native pacbio_native pacbio_native pacbio_native", 4494598972.0, 163482.0, "1 2K D05 1.tar.gz", null, "A:1263523570;C:919488657;G:1005901638;T:1241749988;N:0", null, null, null, null, 1263523570, 919488657, 1005901638, 1241749988, 0, "SRX5108026", "SRS4117494", "SRA820074", "Institute of Hydrobiology, Chinese Academy of Sciences|State Key Laboratory of Freshwater Ecology and Bio", "Institute of Hydrobiology, Chinese Academy of Sciences", null, null, null, null, null, null, null, null, null, null, null, null, null, null, "pacbio", "pacbio_early", "full_length", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2018-12-10", "Zygote", "Embryo", "Gonad", "Reproductive System"], [50514, "SRR8293428", "SRX5108025", "SRS4117494", "SRP172911", "PRJNA498365", "Raw sequence reads in the unfertilized eggs of zebrafish Danio rerio", "PRJNA498365", "Other", "Raw sequence reads of full length transcriptome sequencing in the unfertilized eggs of zebrafish Danio rerio.", null, null, "Three female zebrafish were selected and 100 unfertilized eggs from each female were harvested as three samples Z1 1 Z1 2 and Z1 3 respectively; Identical quantities of RNA from Z1 1 Z1 2 and Z1 3 were combined into sample Z1 M.", "Model organism or animal sample from Danio rerio", "unfertilized eggs from three female zebrafish were harvested as samples Z1 M", null, "isolate:3 female zebrafish were selected and 100 unfertilized eggs from each female were harvested as 3 samples Z1 1 Z1 2 and Z1 3 respectively; and identical quantities of RNA from Z1 1 Z1 2 and Z1 3 were combined into sample Z1 M.|dev stage:unfertilized egg|sex:female|tissue:ovary|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Full length transcriptome sequencing of zebrafish Danio rerio : adult female unfertilized eggs.", "1 2K E05 1", "1 2K E05 1", "RNA sample Z1 M was reverse transcribed into cDNA. Four fragment ranges 0.5 1K 1 2K 2 3K and >3K were prepared according to the PacBio Iso Seq protocol. PacBio RS II sequencing reactions of 16 SMRT single molecule real time cells 3 cells of 0.5 1K 5 cells of 1 2K 5 cells of 2 3K 3 cells of >3K were performed in DNA Sequencing Reagent 4.0 Clontech Mountain View California USA.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "SINGLE", "PACBIO_SMRT", "PacBio RS II", null, "SRP172911", null, null, "m160831_110725_42199_c101016682550000001823228410211624_s1_p0.1.bax.h5 m160831_110725_42199_c101016682550000001823228410211624_s1_p0.2.bax.h5 m160831_110725_42199_c101016682550000001823228410211624_s1_p0.3.bax.h5 m160831_110725_42199_c101016682550000001823228410211624_s1_p0.bas.h5", "pacbio_native pacbio_native pacbio_native pacbio_native", 4610281055.0, 163482.0, "1 2K E05 1.tar.gz", null, "A:1313884618;C:971754386;G:994748647;T:1264950574;N:0", null, null, null, null, 1313884618, 971754386, 994748647, 1264950574, 0, "SRX5108025", "SRS4117494", "SRA820074", "Institute of Hydrobiology, Chinese Academy of Sciences|State Key Laboratory of Freshwater Ecology and Bio", "Institute of Hydrobiology, Chinese Academy of Sciences", null, null, null, null, null, null, null, null, null, null, null, null, null, null, "pacbio", "pacbio_early", "full_length", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2018-12-10", "Zygote", "Embryo", "Gonad", "Reproductive System"], [50515, "SRR8293429", "SRX5108024", "SRS4117494", "SRP172911", "PRJNA498365", "Raw sequence reads in the unfertilized eggs of zebrafish Danio rerio", "PRJNA498365", "Other", "Raw sequence reads of full length transcriptome sequencing in the unfertilized eggs of zebrafish Danio rerio.", null, null, "Three female zebrafish were selected and 100 unfertilized eggs from each female were harvested as three samples Z1 1 Z1 2 and Z1 3 respectively; Identical quantities of RNA from Z1 1 Z1 2 and Z1 3 were combined into sample Z1 M.", "Model organism or animal sample from Danio rerio", "unfertilized eggs from three female zebrafish were harvested as samples Z1 M", null, "isolate:3 female zebrafish were selected and 100 unfertilized eggs from each female were harvested as 3 samples Z1 1 Z1 2 and Z1 3 respectively; and identical quantities of RNA from Z1 1 Z1 2 and Z1 3 were combined into sample Z1 M.|dev stage:unfertilized egg|sex:female|tissue:ovary|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Full length transcriptome sequencing of zebrafish Danio rerio : adult female unfertilized eggs.", "1 2K F03 1", "1 2K F03 1", "RNA sample Z1 M was reverse transcribed into cDNA. Four fragment ranges 0.5 1K 1 2K 2 3K and >3K were prepared according to the PacBio Iso Seq protocol. PacBio RS II sequencing reactions of 16 SMRT single molecule real time cells 3 cells of 0.5 1K 5 cells of 1 2K 5 cells of 2 3K 3 cells of >3K were performed in DNA Sequencing Reagent 4.0 Clontech Mountain View California USA.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "SINGLE", "PACBIO_SMRT", "PacBio RS II", null, "SRP172911", null, null, "m160828_213458_42199_c101055502550000001823247601061765_s1_p0.1.bax.h5 m160828_213458_42199_c101055502550000001823247601061765_s1_p0.2.bax.h5 m160828_213458_42199_c101055502550000001823247601061765_s1_p0.3.bax.h5 m160828_213458_42199_c101055502550000001823247601061765_s1_p0.bas.h5", "pacbio_native pacbio_native pacbio_native pacbio_native", 6488051235.0, 163482.0, "1 2K F03 1.tar.gz", null, "A:1835535517;C:1366315971;G:1464006715;T:1752025312;N:0", null, null, null, null, 1835535517, 1366315971, 1464006715, 1752025312, 0, "SRX5108024", "SRS4117494", "SRA820074", "Institute of Hydrobiology, Chinese Academy of Sciences|State Key Laboratory of Freshwater Ecology and Bio", "Institute of Hydrobiology, Chinese Academy of Sciences", null, null, null, null, null, null, null, null, null, null, null, null, null, null, "pacbio", "pacbio_early", "full_length", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2018-12-10", "Zygote", "Embryo", "Gonad", "Reproductive System"], [50516, "SRR8293430", "SRX5108023", "SRS4117494", "SRP172911", "PRJNA498365", "Raw sequence reads in the unfertilized eggs of zebrafish Danio rerio", "PRJNA498365", "Other", "Raw sequence reads of full length transcriptome sequencing in the unfertilized eggs of zebrafish Danio rerio.", null, null, "Three female zebrafish were selected and 100 unfertilized eggs from each female were harvested as three samples Z1 1 Z1 2 and Z1 3 respectively; Identical quantities of RNA from Z1 1 Z1 2 and Z1 3 were combined into sample Z1 M.", "Model organism or animal sample from Danio rerio", "unfertilized eggs from three female zebrafish were harvested as samples Z1 M", null, "isolate:3 female zebrafish were selected and 100 unfertilized eggs from each female were harvested as 3 samples Z1 1 Z1 2 and Z1 3 respectively; and identical quantities of RNA from Z1 1 Z1 2 and Z1 3 were combined into sample Z1 M.|dev stage:unfertilized egg|sex:female|tissue:ovary|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Full length transcriptome sequencing of zebrafish Danio rerio : adult female unfertilized eggs.", "1 2K G05 1", "1 2K G05 1", "RNA sample Z1 M was reverse transcribed into cDNA. Four fragment ranges 0.5 1K 1 2K 2 3K and >3K were prepared according to the PacBio Iso Seq protocol. PacBio RS II sequencing reactions of 16 SMRT single molecule real time cells 3 cells of 0.5 1K 5 cells of 1 2K 5 cells of 2 3K 3 cells of >3K were performed in DNA Sequencing Reagent 4.0 Clontech Mountain View California USA.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "SINGLE", "PACBIO_SMRT", "PacBio RS II", null, "SRP172911", null, null, "m160829_020016_42199_c101055502550000001823247601061766_s1_p0.1.bax.h5 m160829_020016_42199_c101055502550000001823247601061766_s1_p0.2.bax.h5 m160829_020016_42199_c101055502550000001823247601061766_s1_p0.3.bax.h5 m160829_020016_42199_c101055502550000001823247601061766_s1_p0.bas.h5", "pacbio_native pacbio_native pacbio_native pacbio_native", 6326394753.0, 163482.0, "1 2K G03 1.tar.gz", null, "A:1844148357;C:1410947877;G:1367704673;T:1640545786;N:0", null, null, null, null, 1844148357, 1410947877, 1367704673, 1640545786, 0, "SRX5108023", "SRS4117494", "SRA820074", "Institute of Hydrobiology, Chinese Academy of Sciences|State Key Laboratory of Freshwater Ecology and Bio", "Institute of Hydrobiology, Chinese Academy of Sciences", null, null, null, null, null, null, null, null, null, null, null, null, null, null, "pacbio", "pacbio_early", "full_length", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2018-12-10", "Zygote", "Embryo", "Gonad", "Reproductive System"], [50517, "SRR8293431", "SRX5108022", "SRS4117494", "SRP172911", "PRJNA498365", "Raw sequence reads in the unfertilized eggs of zebrafish Danio rerio", "PRJNA498365", "Other", "Raw sequence reads of full length transcriptome sequencing in the unfertilized eggs of zebrafish Danio rerio.", null, null, "Three female zebrafish were selected and 100 unfertilized eggs from each female were harvested as three samples Z1 1 Z1 2 and Z1 3 respectively; Identical quantities of RNA from Z1 1 Z1 2 and Z1 3 were combined into sample Z1 M.", "Model organism or animal sample from Danio rerio", "unfertilized eggs from three female zebrafish were harvested as samples Z1 M", null, "isolate:3 female zebrafish were selected and 100 unfertilized eggs from each female were harvested as 3 samples Z1 1 Z1 2 and Z1 3 respectively; and identical quantities of RNA from Z1 1 Z1 2 and Z1 3 were combined into sample Z1 M.|dev stage:unfertilized egg|sex:female|tissue:ovary|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Full length transcriptome sequencing of zebrafish Danio rerio : adult female unfertilized eggs.", "0.5 1K E03 1", "0.5 1K E03 1", "RNA sample Z1 M was reverse transcribed into cDNA. Four fragment ranges 0.5 1K 1 2K 2 3K and >3K were prepared according to the PacBio Iso Seq protocol. 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Four fragment ranges 0.5 1K 1 2K 2 3K and >3K were prepared according to the PacBio Iso Seq protocol. 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Four fragment ranges 0.5 1K 1 2K 2 3K and >3K were prepared according to the PacBio Iso Seq protocol. PacBio RS II sequencing reactions of 16 SMRT single molecule real time cells 3 cells of 0.5 1K 5 cells of 1 2K 5 cells of 2 3K 3 cells of >3K were performed in DNA Sequencing Reagent 4.0 Clontech Mountain View California USA.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "SINGLE", "PACBIO_SMRT", "PacBio RS II", null, "SRP172911", null, null, "m160831_022615_42199_c101016682550000001823228410211622_s1_p0.1.bax.h5 m160831_022615_42199_c101016682550000001823228410211622_s1_p0.2.bax.h5 m160831_022615_42199_c101016682550000001823228410211622_s1_p0.3.bax.h5 m160831_022615_42199_c101016682550000001823228410211622_s1_p0.bas.h5", "pacbio_native pacbio_native pacbio_native pacbio_native", 3047073997.0, 163482.0, "1 2K C05 1.tar.gz", null, "A:862003813;C:632482019;G:660595007;T:843966997;N:0", null, null, null, null, 862003813, 632482019, 660595007, 843966997, 0, "SRX5108019", "SRS4117494", "SRA820074", "Institute of Hydrobiology, Chinese Academy of Sciences|State Key Laboratory of Freshwater Ecology and Bio", "Institute of Hydrobiology, Chinese Academy of Sciences", null, null, null, null, null, null, null, null, null, null, null, null, null, null, "pacbio", "pacbio_early", "full_length", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2018-12-10", "Zygote", "Embryo", "Gonad", "Reproductive System"], [50521, "SRR8293435", "SRX5108018", "SRS4117494", "SRP172911", "PRJNA498365", "Raw sequence reads in the unfertilized eggs of zebrafish Danio rerio", "PRJNA498365", "Other", "Raw sequence reads of full length transcriptome sequencing in the unfertilized eggs of zebrafish Danio rerio.", null, null, "Three female zebrafish were selected and 100 unfertilized eggs from each female were harvested as three samples Z1 1 Z1 2 and Z1 3 respectively; Identical quantities of RNA from Z1 1 Z1 2 and Z1 3 were combined into sample Z1 M.", "Model organism or animal sample from Danio rerio", "unfertilized eggs from three female zebrafish were harvested as samples Z1 M", null, "isolate:3 female zebrafish were selected and 100 unfertilized eggs from each female were harvested as 3 samples Z1 1 Z1 2 and Z1 3 respectively; and identical quantities of RNA from Z1 1 Z1 2 and Z1 3 were combined into sample Z1 M.|dev stage:unfertilized egg|sex:female|tissue:ovary|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Full length transcriptome sequencing of zebrafish Danio rerio : adult female unfertilized eggs.", "2 3K F05 1", "2 3K F05 1", "RNA sample Z1 M was reverse transcribed into cDNA. 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PacBio RS II sequencing reactions of 16 SMRT single molecule real time cells 3 cells of 0.5 1K 5 cells of 1 2K 5 cells of 2 3K 3 cells of >3K were performed in DNA Sequencing Reagent 4.0 Clontech Mountain View California USA.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "SINGLE", "PACBIO_SMRT", "PacBio RS II", null, "SRP172911", null, null, "m160831_152721_42199_c101016682550000001823228410211625_s1_p0.1.bax.h5 m160831_152721_42199_c101016682550000001823228410211625_s1_p0.2.bax.h5 m160831_152721_42199_c101016682550000001823228410211625_s1_p0.3.bax.h5 m160831_152721_42199_c101016682550000001823228410211625_s1_p0.bas.h5", "pacbio_native pacbio_native pacbio_native pacbio_native", 3504827488.0, 163482.0, "2 3K F05 1.tar.gz", null, "A:1035191904;C:787763869;G:735456262;T:916015813;N:0", null, null, null, null, 1035191904, 787763869, 735456262, 916015813, 0, "SRX5108018", "SRS4117494", "SRA820074", "Institute of Hydrobiology, Chinese Academy of Sciences|State Key Laboratory of Freshwater Ecology and Bio", "Institute of Hydrobiology, Chinese Academy of Sciences", null, null, null, null, null, null, null, null, null, null, null, null, null, null, "pacbio", "pacbio_early", "full_length", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2018-12-10", "Zygote", "Embryo", "Gonad", "Reproductive System"], [50522, "SRR8293436", "SRX5108017", "SRS4117494", "SRP172911", "PRJNA498365", "Raw sequence reads in the unfertilized eggs of zebrafish Danio rerio", "PRJNA498365", "Other", "Raw sequence reads of full length transcriptome sequencing in the unfertilized eggs of zebrafish Danio rerio.", null, null, "Three female zebrafish were selected and 100 unfertilized eggs from each female were harvested as three samples Z1 1 Z1 2 and Z1 3 respectively; Identical quantities of RNA from Z1 1 Z1 2 and Z1 3 were combined into sample Z1 M.", "Model organism or animal sample from Danio rerio", "unfertilized eggs from three female zebrafish were harvested as samples Z1 M", null, "isolate:3 female zebrafish were selected and 100 unfertilized eggs from each female were harvested as 3 samples Z1 1 Z1 2 and Z1 3 respectively; and identical quantities of RNA from Z1 1 Z1 2 and Z1 3 were combined into sample Z1 M.|dev stage:unfertilized egg|sex:female|tissue:ovary|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Full length transcriptome sequencing of zebrafish Danio rerio : adult female unfertilized eggs.", "2 3K G05 1", "2 3K G05 1", "RNA sample Z1 M was reverse transcribed into cDNA. Four fragment ranges 0.5 1K 1 2K 2 3K and >3K were prepared according to the PacBio Iso Seq protocol. PacBio RS II sequencing reactions of 16 SMRT single molecule real time cells 3 cells of 0.5 1K 5 cells of 1 2K 5 cells of 2 3K 3 cells of >3K were performed in DNA Sequencing Reagent 4.0 Clontech Mountain View California USA.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "SINGLE", "PACBIO_SMRT", "PacBio RS II", null, "SRP172911", null, null, "m160831_195005_42199_c101016682550000001823228410211626_s1_p0.1.bax.h5 m160831_195005_42199_c101016682550000001823228410211626_s1_p0.2.bax.h5 m160831_195005_42199_c101016682550000001823228410211626_s1_p0.3.bax.h5 m160831_195005_42199_c101016682550000001823228410211626_s1_p0.bas.h5", "pacbio_native pacbio_native pacbio_native pacbio_native", 3325636539.0, 163482.0, "2 3K G05 1.tar.gz", null, "A:945624516;C:712334057;G:726829680;T:910995593;N:0", null, null, null, null, 945624516, 712334057, 726829680, 910995593, 0, "SRX5108017", "SRS4117494", "SRA820074", "Institute of Hydrobiology, Chinese Academy of Sciences|State Key Laboratory of Freshwater Ecology and Bio", "Institute of Hydrobiology, Chinese Academy of Sciences", null, null, null, null, null, null, null, null, null, null, null, null, null, null, "pacbio", "pacbio_early", "full_length", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2018-12-10", "Zygote", "Embryo", "Gonad", "Reproductive System"], [50523, "SRR8293437", "SRX5108016", "SRS4117494", "SRP172911", "PRJNA498365", "Raw sequence reads in the unfertilized eggs of zebrafish Danio rerio", "PRJNA498365", "Other", "Raw sequence reads of full length transcriptome sequencing in the unfertilized eggs of zebrafish Danio rerio.", null, null, "Three female zebrafish were selected and 100 unfertilized eggs from each female were harvested as three samples Z1 1 Z1 2 and Z1 3 respectively; Identical quantities of RNA from Z1 1 Z1 2 and Z1 3 were combined into sample Z1 M.", "Model organism or animal sample from Danio rerio", "unfertilized eggs from three female zebrafish were harvested as samples Z1 M", null, "isolate:3 female zebrafish were selected and 100 unfertilized eggs from each female were harvested as 3 samples Z1 1 Z1 2 and Z1 3 respectively; and identical quantities of RNA from Z1 1 Z1 2 and Z1 3 were combined into sample Z1 M.|dev stage:unfertilized egg|sex:female|tissue:ovary|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Full length transcriptome sequencing of zebrafish Danio rerio : adult female unfertilized eggs.", "2 3K H05 1", "2 3K H05 1", "RNA sample Z1 M was reverse transcribed into cDNA. Four fragment ranges 0.5 1K 1 2K 2 3K and >3K were prepared according to the PacBio Iso Seq protocol. PacBio RS II sequencing reactions of 16 SMRT single molecule real time cells 3 cells of 0.5 1K 5 cells of 1 2K 5 cells of 2 3K 3 cells of >3K were performed in DNA Sequencing Reagent 4.0 Clontech Mountain View California USA.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "SINGLE", "PACBIO_SMRT", "PacBio RS II", null, "SRP172911", null, null, "m160901_000924_42199_c101016682550000001823228410211627_s1_p0.1.bax.h5 m160901_000924_42199_c101016682550000001823228410211627_s1_p0.2.bax.h5 m160901_000924_42199_c101016682550000001823228410211627_s1_p0.3.bax.h5 m160901_000924_42199_c101016682550000001823228410211627_s1_p0.bas.h5", "pacbio_native pacbio_native pacbio_native pacbio_native", 3219471171.0, 163482.0, "2 3K H05 1.tar.gz", null, "A:955121582;C:717645710;G:671893655;T:846270302;N:0", null, null, null, null, 955121582, 717645710, 671893655, 846270302, 0, "SRX5108016", "SRS4117494", "SRA820074", "Institute of Hydrobiology, Chinese Academy of Sciences|State Key Laboratory of Freshwater Ecology and Bio", "Institute of Hydrobiology, Chinese Academy of Sciences", null, null, null, null, null, null, null, null, null, null, null, null, null, null, "pacbio", "pacbio_early", "full_length", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2018-12-10", "Zygote", "Embryo", "Gonad", "Reproductive System"], [50524, "SRR8293438", "SRX5108015", "SRS4117494", "SRP172911", "PRJNA498365", "Raw sequence reads in the unfertilized eggs of zebrafish Danio rerio", "PRJNA498365", "Other", "Raw sequence reads of full length transcriptome sequencing in the unfertilized eggs of zebrafish Danio rerio.", null, null, "Three female zebrafish were selected and 100 unfertilized eggs from each female were harvested as three samples Z1 1 Z1 2 and Z1 3 respectively; Identical quantities of RNA from Z1 1 Z1 2 and Z1 3 were combined into sample Z1 M.", "Model organism or animal sample from Danio rerio", "unfertilized eggs from three female zebrafish were harvested as samples Z1 M", null, "isolate:3 female zebrafish were selected and 100 unfertilized eggs from each female were harvested as 3 samples Z1 1 Z1 2 and Z1 3 respectively; and identical quantities of RNA from Z1 1 Z1 2 and Z1 3 were combined into sample Z1 M.|dev stage:unfertilized egg|sex:female|tissue:ovary|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Full length transcriptome sequencing of zebrafish Danio rerio : adult female unfertilized eggs.", "3K plus C03 1", "3K plus C03 1", "RNA sample Z1 M was reverse transcribed into cDNA. Four fragment ranges 0.5 1K 1 2K 2 3K and >3K were prepared according to the PacBio Iso Seq protocol. 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