{"database": "metadata", "table": "run_metadata", "is_view": false, "human_description_en": "where experiment.library_selection = \"RANDOM\", technology = \"unknown\" and tissue_curation_coarse = \"Respiratory System\"", "rows": [[9842, "ERR4029259", "ERX4030575", "ERS4514128", "ERP121190", "PRJEB37851", "Zebrafish il 4 il 10 regulated immunity in gills", "E-MTAB-8958", "Transcriptome Analysis", "The evolutionary origin of vertebrate type 2 immunity is a topic of great interest. Several studies have focused on the immune cell components of evolutionary older vertebrates such as fish. However  how fish cytokines function and whether they have similar roles as in mammals is still a matter of speculation. Here  we have used the zebrafish Danio rerio to gain insights into il4/13a and il4/13b genes and characterized their role under both homeostatic and inflammatory conditions. We established knockouts for both il4/13a and il4/13b genes and showed that they are needed to suppress inflammation in larvae and in the gill mucosa. As a counterpoint  we examined the gills of il10 defective zebrafish and revealed the importance of il10 in maintaining homeostasis in this mucosal tissue. As in mammals  zebrafish il10 appears to have an anti inflammatory function.", "ENA FIRST PUBLIC:2020 05 28|ENA LAST UPDATE:2020 04 16", null, "Protocols: This submission contains RNA seq samples from the gill tissue of five zebrafish strains: Wildtype  il4/13a KO  il4/13b KO  il4/13a;b KO and il10 KO. The strains il4/13a KO  il4/13b KO and il4/13a;b KO were generated using CRISPR/Cas9 system whereas the il10 KO was generated by the Sanger Institute through the Zebrafish Mutation Project Kettleborough RNW  Busch nentwich EM  Harvey SA  Dooley CM  Bruijn E De  Eeden F Van  et al. Europe PMC Funders Group Europe PMC Funders Author Manuscripts A systematic genome wide analysis of zebrafish protein coding gene function. 2013;4967446:494 7. Zebrafish Danio rerio were maintained under standard conditions 28 \u00b0C under a 14 h light/10 h dark cycle within the Biological Services Unit The University of Manchester. Gill tissue was harvested from adult 6 mpf male and female zebrafish. Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen. Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen. Prior to performing RNA seq  the integrity of RNA samples was assessed using a 2200 TapeStation Agilent Technologies according to the manufacturer's instructions. Truseq stranded mRNA Assay. Paired end.", "Sample 4", "SAMEA6786449", "UNIVERSITY OF MANCHESTER", "ENA FIRST PUBLIC:2020 05 28T17:07:47Z|ENA LAST UPDATE:2020 04 16T14:58:15Z|External Id:SAMEA6786449|INSDC center name:UNIVERSITY OF MANCHESTER|INSDC first public:2020 05 28T17:07:47Z|INSDC last update:2020 04 16T14:58:15Z|INSDC status:public|Submitter Id:E MTAB 8958:Sample 4|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:wild type genotype|organism part:gill|sample name:E MTAB 8958:Sample 4|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Illumina HiSeq 4000 paired end sequencing; Zebrafish il 4 il 10 regulated immunity in gills", "E MTAB 8958:Sample 4 p", "Sample 4 p", "Zebrafish il 4 il 10 regulated immunity in gills", "This submission contains RNA seq samples from the gill tissue of five zebrafish strains: Wildtype  il4/13a KO  il4/13b KO  il4/13a;b KO and il10 KO. The strains il4/13a KO  il4/13b KO and il4/13a;b KO were generated using CRISPR/Cas9 system whereas the  il10 KO was generated by the Sanger Institute through the Zebrafish Mutation Project Kettleborough RNW  Busch nentwich EM  Harvey SA  Dooley CM  Bruijn E De  Eeden F Van  et al. Europe PMC Funders Group Europe PMC Funders Author Manuscripts A systematic genome wide analysis of zebrafish protein coding gene function. 2013;4967446:494\u20137. Zebrafish Danio rerio were maintained under standard conditions 28 \u00b0C under a 14 h light/10 h dark cycle within the Biological Services Unit The University of Manchester. Gill tissue was harvested from adult 6 mpf male and female zebrafish. Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen.  Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen. Prior to performing RNA seq  the integrity of RNA samples was assessed using a 2200 TapeStation Agilent Technologies according to the manufacturer's instructions.  Truseq stranded mRNA Assay. Paired end.", "Experimental Factor: genotype:wild type genotype", "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "ERP121190", "Illumina HiSeq 4000 paired end sequencing; Zebrafish il 4 il 10 regulated immunity in gills", "ENA FIRST PUBLIC:2020 05 28|ENA LAST UPDATE:2020 04 16", "FB4_S41_R1_001.fastq.gz FB4_S41_R2_001.fastq.gz", "fastq fastq", 4354809436.0, 29071030.0, "E MTAB 8958:FB4 S41 R", "0:74.90 1:74.90", "A:1140431810;C:1005396495;G:1008528915;T:1171220102;N:29232114", 74, 74, null, null, 1140431810, 1005396495, 1008528915, 1171220102, 29232114, "ERX4030575", "ERS4514128", "ERA2508092", "UNIVERSITY OF MANCHESTER|European Nucleotide Archive", "UNIVERSITY OF MANCHESTER|European Nucleotide Archive", 2, 0.91143, 0.91076, 0.10123, 0.09941, 0.68235, 0.68452, 0.48713, 0.48998, 76, 74, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "trueseq", "bulk", "unknown", "unknown", null, "United Kingdom", "2020-04-16", "Adult", "Adult", "Gill", "Respiratory System"], [9843, "ERR4029258", "ERX4030574", "ERS4514127", "ERP121190", "PRJEB37851", "Zebrafish il 4 il 10 regulated immunity in gills", "E-MTAB-8958", "Transcriptome Analysis", "The evolutionary origin of vertebrate type 2 immunity is a topic of great interest. Several studies have focused on the immune cell components of evolutionary older vertebrates such as fish. However  how fish cytokines function and whether they have similar roles as in mammals is still a matter of speculation. Here  we have used the zebrafish Danio rerio to gain insights into il4/13a and il4/13b genes and characterized their role under both homeostatic and inflammatory conditions. We established knockouts for both il4/13a and il4/13b genes and showed that they are needed to suppress inflammation in larvae and in the gill mucosa. As a counterpoint  we examined the gills of il10 defective zebrafish and revealed the importance of il10 in maintaining homeostasis in this mucosal tissue. As in mammals  zebrafish il10 appears to have an anti inflammatory function.", "ENA FIRST PUBLIC:2020 05 28|ENA LAST UPDATE:2020 04 16", null, "Protocols: This submission contains RNA seq samples from the gill tissue of five zebrafish strains: Wildtype  il4/13a KO  il4/13b KO  il4/13a;b KO and il10 KO. The strains il4/13a KO  il4/13b KO and il4/13a;b KO were generated using CRISPR/Cas9 system whereas the il10 KO was generated by the Sanger Institute through the Zebrafish Mutation Project Kettleborough RNW  Busch nentwich EM  Harvey SA  Dooley CM  Bruijn E De  Eeden F Van  et al. Europe PMC Funders Group Europe PMC Funders Author Manuscripts A systematic genome wide analysis of zebrafish protein coding gene function. 2013;4967446:494 7. Zebrafish Danio rerio were maintained under standard conditions 28 \u00b0C under a 14 h light/10 h dark cycle within the Biological Services Unit The University of Manchester. Gill tissue was harvested from adult 6 mpf male and female zebrafish. Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen. Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen. Prior to performing RNA seq  the integrity of RNA samples was assessed using a 2200 TapeStation Agilent Technologies according to the manufacturer's instructions. Truseq stranded mRNA Assay. Paired end.", "Sample 3", "SAMEA6786448", "UNIVERSITY OF MANCHESTER", "ENA FIRST PUBLIC:2020 05 28T17:07:47Z|ENA LAST UPDATE:2020 04 16T14:58:15Z|External Id:SAMEA6786448|INSDC center name:UNIVERSITY OF MANCHESTER|INSDC first public:2020 05 28T17:07:47Z|INSDC last update:2020 04 16T14:58:15Z|INSDC status:public|Submitter Id:E MTAB 8958:Sample 3|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:wild type genotype|organism part:gill|sample name:E MTAB 8958:Sample 3|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Illumina HiSeq 4000 paired end sequencing; Zebrafish il 4 il 10 regulated immunity in gills", "E MTAB 8958:Sample 3 p", "Sample 3 p", "Zebrafish il 4 il 10 regulated immunity in gills", "This submission contains RNA seq samples from the gill tissue of five zebrafish strains: Wildtype  il4/13a KO  il4/13b KO  il4/13a;b KO and il10 KO. The strains il4/13a KO  il4/13b KO and il4/13a;b KO were generated using CRISPR/Cas9 system whereas the  il10 KO was generated by the Sanger Institute through the Zebrafish Mutation Project Kettleborough RNW  Busch nentwich EM  Harvey SA  Dooley CM  Bruijn E De  Eeden F Van  et al. Europe PMC Funders Group Europe PMC Funders Author Manuscripts A systematic genome wide analysis of zebrafish protein coding gene function. 2013;4967446:494\u20137. Zebrafish Danio rerio were maintained under standard conditions 28 \u00b0C under a 14 h light/10 h dark cycle within the Biological Services Unit The University of Manchester. Gill tissue was harvested from adult 6 mpf male and female zebrafish. Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen.  Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen. Prior to performing RNA seq  the integrity of RNA samples was assessed using a 2200 TapeStation Agilent Technologies according to the manufacturer's instructions.  Truseq stranded mRNA Assay. Paired end.", "Experimental Factor: genotype:wild type genotype", "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "ERP121190", "Illumina HiSeq 4000 paired end sequencing; Zebrafish il 4 il 10 regulated immunity in gills", "ENA FIRST PUBLIC:2020 05 28|ENA LAST UPDATE:2020 04 16", "FB3_S40_R1_001.fastq.gz FB3_S40_R2_001.fastq.gz", "fastq fastq", 3973860397.0, 26559713.0, "E MTAB 8958:FB3 S40 R", "0:74.80 1:74.82", "A:1038749031;C:917944667;G:920080717;T:1066160216;N:30925766", 74, 74, null, null, 1038749031, 917944667, 920080717, 1066160216, 30925766, "ERX4030574", "ERS4514127", "ERA2508092", "UNIVERSITY OF MANCHESTER|European Nucleotide Archive", "UNIVERSITY OF MANCHESTER|European Nucleotide Archive", 2, 0.91065, 0.90981, 0.10133, 0.09911, 0.68789, 0.68945, 0.47182, 0.49996, 76, 76, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "trueseq", "bulk", "unknown", "unknown", null, "United Kingdom", "2020-04-16", "Adult", "Adult", "Gill", "Respiratory System"], [9844, "ERR4029257", "ERX4030573", "ERS4514126", "ERP121190", "PRJEB37851", "Zebrafish il 4 il 10 regulated immunity in gills", "E-MTAB-8958", "Transcriptome Analysis", "The evolutionary origin of vertebrate type 2 immunity is a topic of great interest. Several studies have focused on the immune cell components of evolutionary older vertebrates such as fish. However  how fish cytokines function and whether they have similar roles as in mammals is still a matter of speculation. Here  we have used the zebrafish Danio rerio to gain insights into il4/13a and il4/13b genes and characterized their role under both homeostatic and inflammatory conditions. We established knockouts for both il4/13a and il4/13b genes and showed that they are needed to suppress inflammation in larvae and in the gill mucosa. As a counterpoint  we examined the gills of il10 defective zebrafish and revealed the importance of il10 in maintaining homeostasis in this mucosal tissue. As in mammals  zebrafish il10 appears to have an anti inflammatory function.", "ENA FIRST PUBLIC:2020 05 28|ENA LAST UPDATE:2020 04 16", null, "Protocols: This submission contains RNA seq samples from the gill tissue of five zebrafish strains: Wildtype  il4/13a KO  il4/13b KO  il4/13a;b KO and il10 KO. The strains il4/13a KO  il4/13b KO and il4/13a;b KO were generated using CRISPR/Cas9 system whereas the il10 KO was generated by the Sanger Institute through the Zebrafish Mutation Project Kettleborough RNW  Busch nentwich EM  Harvey SA  Dooley CM  Bruijn E De  Eeden F Van  et al. Europe PMC Funders Group Europe PMC Funders Author Manuscripts A systematic genome wide analysis of zebrafish protein coding gene function. 2013;4967446:494 7. Zebrafish Danio rerio were maintained under standard conditions 28 \u00b0C under a 14 h light/10 h dark cycle within the Biological Services Unit The University of Manchester. Gill tissue was harvested from adult 6 mpf male and female zebrafish. Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen. Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen. Prior to performing RNA seq  the integrity of RNA samples was assessed using a 2200 TapeStation Agilent Technologies according to the manufacturer's instructions. Truseq stranded mRNA Assay. Paired end.", "Sample 2", "SAMEA6786447", "UNIVERSITY OF MANCHESTER", "ENA FIRST PUBLIC:2020 05 28T17:07:47Z|ENA LAST UPDATE:2020 04 16T14:58:15Z|External Id:SAMEA6786447|INSDC center name:UNIVERSITY OF MANCHESTER|INSDC first public:2020 05 28T17:07:47Z|INSDC last update:2020 04 16T14:58:15Z|INSDC status:public|Submitter Id:E MTAB 8958:Sample 2|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:wild type genotype|organism part:gill|sample name:E MTAB 8958:Sample 2|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Illumina HiSeq 4000 paired end sequencing; Zebrafish il 4 il 10 regulated immunity in gills", "E MTAB 8958:Sample 2 p", "Sample 2 p", "Zebrafish il 4 il 10 regulated immunity in gills", "This submission contains RNA seq samples from the gill tissue of five zebrafish strains: Wildtype  il4/13a KO  il4/13b KO  il4/13a;b KO and il10 KO. The strains il4/13a KO  il4/13b KO and il4/13a;b KO were generated using CRISPR/Cas9 system whereas the  il10 KO was generated by the Sanger Institute through the Zebrafish Mutation Project Kettleborough RNW  Busch nentwich EM  Harvey SA  Dooley CM  Bruijn E De  Eeden F Van  et al. Europe PMC Funders Group Europe PMC Funders Author Manuscripts A systematic genome wide analysis of zebrafish protein coding gene function. 2013;4967446:494\u20137. Zebrafish Danio rerio were maintained under standard conditions 28 \u00b0C under a 14 h light/10 h dark cycle within the Biological Services Unit The University of Manchester. Gill tissue was harvested from adult 6 mpf male and female zebrafish. Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen.  Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen. Prior to performing RNA seq  the integrity of RNA samples was assessed using a 2200 TapeStation Agilent Technologies according to the manufacturer's instructions.  Truseq stranded mRNA Assay. Paired end.", "Experimental Factor: genotype:wild type genotype", "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "ERP121190", "Illumina HiSeq 4000 paired end sequencing; Zebrafish il 4 il 10 regulated immunity in gills", "ENA FIRST PUBLIC:2020 05 28|ENA LAST UPDATE:2020 04 16", "FB2_S39_R1_001.fastq.gz FB2_S39_R2_001.fastq.gz", "fastq fastq", 4135184845.0, 27517010.0, "E MTAB 8958:FB2 S39 R", "0:75.15 1:75.13", "A:1084253902;C:962303819;G:961163738;T:1111949967;N:15513419", 75, 75, null, null, 1084253902, 962303819, 961163738, 1111949967, 15513419, "ERX4030573", "ERS4514126", "ERA2508092", "UNIVERSITY OF MANCHESTER|European Nucleotide Archive", "UNIVERSITY OF MANCHESTER|European Nucleotide Archive", 2, 0.91098, 0.91057, 0.09468, 0.09311, 0.68095, 0.68282, 0.49982, 0.50146, 75, 75, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "trueseq", "bulk", "unknown", "unknown", null, "United Kingdom", "2020-04-16", "Adult", "Adult", "Gill", "Respiratory System"], [9845, "ERR4029256", "ERX4030572", "ERS4514125", "ERP121190", "PRJEB37851", "Zebrafish il 4 il 10 regulated immunity in gills", "E-MTAB-8958", "Transcriptome Analysis", "The evolutionary origin of vertebrate type 2 immunity is a topic of great interest. Several studies have focused on the immune cell components of evolutionary older vertebrates such as fish. However  how fish cytokines function and whether they have similar roles as in mammals is still a matter of speculation. Here  we have used the zebrafish Danio rerio to gain insights into il4/13a and il4/13b genes and characterized their role under both homeostatic and inflammatory conditions. We established knockouts for both il4/13a and il4/13b genes and showed that they are needed to suppress inflammation in larvae and in the gill mucosa. As a counterpoint  we examined the gills of il10 defective zebrafish and revealed the importance of il10 in maintaining homeostasis in this mucosal tissue. As in mammals  zebrafish il10 appears to have an anti inflammatory function.", "ENA FIRST PUBLIC:2020 05 28|ENA LAST UPDATE:2020 04 16", null, "Protocols: This submission contains RNA seq samples from the gill tissue of five zebrafish strains: Wildtype  il4/13a KO  il4/13b KO  il4/13a;b KO and il10 KO. The strains il4/13a KO  il4/13b KO and il4/13a;b KO were generated using CRISPR/Cas9 system whereas the il10 KO was generated by the Sanger Institute through the Zebrafish Mutation Project Kettleborough RNW  Busch nentwich EM  Harvey SA  Dooley CM  Bruijn E De  Eeden F Van  et al. Europe PMC Funders Group Europe PMC Funders Author Manuscripts A systematic genome wide analysis of zebrafish protein coding gene function. 2013;4967446:494 7. Zebrafish Danio rerio were maintained under standard conditions 28 \u00b0C under a 14 h light/10 h dark cycle within the Biological Services Unit The University of Manchester. Gill tissue was harvested from adult 6 mpf male and female zebrafish. Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen. Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen. Prior to performing RNA seq  the integrity of RNA samples was assessed using a 2200 TapeStation Agilent Technologies according to the manufacturer's instructions. Truseq stranded mRNA Assay. Paired end.", "Sample 1", "SAMEA6786446", "UNIVERSITY OF MANCHESTER", "ENA FIRST PUBLIC:2020 05 28T17:07:47Z|ENA LAST UPDATE:2020 04 16T14:58:15Z|External Id:SAMEA6786446|INSDC center name:UNIVERSITY OF MANCHESTER|INSDC first public:2020 05 28T17:07:47Z|INSDC last update:2020 04 16T14:58:15Z|INSDC status:public|Submitter Id:E MTAB 8958:Sample 1|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:wild type genotype|organism part:gill|sample name:E MTAB 8958:Sample 1|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Illumina HiSeq 4000 paired end sequencing; Zebrafish il 4 il 10 regulated immunity in gills", "E MTAB 8958:Sample 1 p", "Sample 1 p", "Zebrafish il 4 il 10 regulated immunity in gills", "This submission contains RNA seq samples from the gill tissue of five zebrafish strains: Wildtype  il4/13a KO  il4/13b KO  il4/13a;b KO and il10 KO. The strains il4/13a KO  il4/13b KO and il4/13a;b KO were generated using CRISPR/Cas9 system whereas the  il10 KO was generated by the Sanger Institute through the Zebrafish Mutation Project Kettleborough RNW  Busch nentwich EM  Harvey SA  Dooley CM  Bruijn E De  Eeden F Van  et al. Europe PMC Funders Group Europe PMC Funders Author Manuscripts A systematic genome wide analysis of zebrafish protein coding gene function. 2013;4967446:494\u20137. Zebrafish Danio rerio were maintained under standard conditions 28 \u00b0C under a 14 h light/10 h dark cycle within the Biological Services Unit The University of Manchester. Gill tissue was harvested from adult 6 mpf male and female zebrafish. Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen.  Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen. Prior to performing RNA seq  the integrity of RNA samples was assessed using a 2200 TapeStation Agilent Technologies according to the manufacturer's instructions.  Truseq stranded mRNA Assay. Paired end.", "Experimental Factor: genotype:wild type genotype", "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "ERP121190", "Illumina HiSeq 4000 paired end sequencing; Zebrafish il 4 il 10 regulated immunity in gills", "ENA FIRST PUBLIC:2020 05 28|ENA LAST UPDATE:2020 06 17", "FB1_S38_R1_001.fastq FB1_S38_R2_001.fastq", "fastq fastq", 4651073045.0, 31021879.0, "E MTAB 8958:FB1 S38 R", "0:74.96 1:74.97", "A:1225305023;C:1072643957;G:1069905162;T:1255950159;N:27268744", 74, 74, null, null, 1225305023, 1072643957, 1069905162, 1255950159, 27268744, "ERX4030572", "ERS4514125", "ERA2508092", "UNIVERSITY OF MANCHESTER|European Nucleotide Archive", "UNIVERSITY OF MANCHESTER|European Nucleotide Archive", 2, 0.91441, 0.91282, 0.10095, 0.09913, 0.686, 0.68745, 0.49433, 0.5047, 76, 76, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "trueseq", "bulk", "unknown", "unknown", null, "United Kingdom", "2020-04-16", "Adult", "Adult", "Gill", "Respiratory System"], [9846, "ERR4029255", "ERX4030571", "ERS4514124", "ERP121190", "PRJEB37851", "Zebrafish il 4 il 10 regulated immunity in gills", "E-MTAB-8958", "Transcriptome Analysis", "The evolutionary origin of vertebrate type 2 immunity is a topic of great interest. Several studies have focused on the immune cell components of evolutionary older vertebrates such as fish. However  how fish cytokines function and whether they have similar roles as in mammals is still a matter of speculation. Here  we have used the zebrafish Danio rerio to gain insights into il4/13a and il4/13b genes and characterized their role under both homeostatic and inflammatory conditions. We established knockouts for both il4/13a and il4/13b genes and showed that they are needed to suppress inflammation in larvae and in the gill mucosa. As a counterpoint  we examined the gills of il10 defective zebrafish and revealed the importance of il10 in maintaining homeostasis in this mucosal tissue. As in mammals  zebrafish il10 appears to have an anti inflammatory function.", "ENA FIRST PUBLIC:2020 05 28|ENA LAST UPDATE:2020 04 16", null, "Protocols: This submission contains RNA seq samples from the gill tissue of five zebrafish strains: Wildtype  il4/13a KO  il4/13b KO  il4/13a;b KO and il10 KO. The strains il4/13a KO  il4/13b KO and il4/13a;b KO were generated using CRISPR/Cas9 system whereas the il10 KO was generated by the Sanger Institute through the Zebrafish Mutation Project Kettleborough RNW  Busch nentwich EM  Harvey SA  Dooley CM  Bruijn E De  Eeden F Van  et al. Europe PMC Funders Group Europe PMC Funders Author Manuscripts A systematic genome wide analysis of zebrafish protein coding gene function. 2013;4967446:494 7. Zebrafish Danio rerio were maintained under standard conditions 28 \u00b0C under a 14 h light/10 h dark cycle within the Biological Services Unit The University of Manchester. Gill tissue was harvested from adult 6 mpf male and female zebrafish. Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen. Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen. Prior to performing RNA seq  the integrity of RNA samples was assessed using a 2200 TapeStation Agilent Technologies according to the manufacturer's instructions. Truseq stranded mRNA Assay. Paired end.", "Sample 16", "SAMEA6786445", "UNIVERSITY OF MANCHESTER", "ENA FIRST PUBLIC:2020 05 28T17:07:47Z|ENA LAST UPDATE:2020 04 16T14:58:15Z|External Id:SAMEA6786445|INSDC center name:UNIVERSITY OF MANCHESTER|INSDC first public:2020 05 28T17:07:47Z|INSDC last update:2020 04 16T14:58:15Z|INSDC status:public|Submitter Id:E MTAB 8958:Sample 16|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:il4/13b  / |organism part:gill|sample name:E MTAB 8958:Sample 16|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Illumina HiSeq 4000 paired end sequencing; Zebrafish il 4 il 10 regulated immunity in gills", "E MTAB 8958:Sample 16 p", "Sample 16 p", "Zebrafish il 4 il 10 regulated immunity in gills", "This submission contains RNA seq samples from the gill tissue of five zebrafish strains: Wildtype  il4/13a KO  il4/13b KO  il4/13a;b KO and il10 KO. The strains il4/13a KO  il4/13b KO and il4/13a;b KO were generated using CRISPR/Cas9 system whereas the  il10 KO was generated by the Sanger Institute through the Zebrafish Mutation Project Kettleborough RNW  Busch nentwich EM  Harvey SA  Dooley CM  Bruijn E De  Eeden F Van  et al. Europe PMC Funders Group Europe PMC Funders Author Manuscripts A systematic genome wide analysis of zebrafish protein coding gene function. 2013;4967446:494\u20137. Zebrafish Danio rerio were maintained under standard conditions 28 \u00b0C under a 14 h light/10 h dark cycle within the Biological Services Unit The University of Manchester. Gill tissue was harvested from adult 6 mpf male and female zebrafish. Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen.  Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen. Prior to performing RNA seq  the integrity of RNA samples was assessed using a 2200 TapeStation Agilent Technologies according to the manufacturer's instructions.  Truseq stranded mRNA Assay. Paired end.", "Experimental Factor: genotype:il4/13b  / ", "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "ERP121190", "Illumina HiSeq 4000 paired end sequencing; Zebrafish il 4 il 10 regulated immunity in gills", "ENA FIRST PUBLIC:2020 05 28|ENA LAST UPDATE:2020 04 16", "FB4_S20_R1_001.fastq.gz FB4_S20_R2_001.fastq.gz", "fastq fastq", 5961540086.0, 39717871.0, "E MTAB 8958:FB4 S20 R", "0:75.07 1:75.03", "A:1565483441;C:1390303898;G:1372759826;T:1611361282;N:21631639", 75, 75, null, null, 1565483441, 1390303898, 1372759826, 1611361282, 21631639, "ERX4030571", "ERS4514124", "ERA2508092", "UNIVERSITY OF MANCHESTER|European Nucleotide Archive", "UNIVERSITY OF MANCHESTER|European Nucleotide Archive", 2, 0.90959, 0.90989, 0.0925, 0.09172, 0.68787, 0.68929, 0.49093, 0.49057, 76, 76, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "trueseq", "bulk", "unknown", "unknown", null, "United Kingdom", "2020-04-16", "Adult", "Adult", "Gill", "Respiratory System"], [9847, "ERR4029254", "ERX4030570", "ERS4514123", "ERP121190", "PRJEB37851", "Zebrafish il 4 il 10 regulated immunity in gills", "E-MTAB-8958", "Transcriptome Analysis", "The evolutionary origin of vertebrate type 2 immunity is a topic of great interest. Several studies have focused on the immune cell components of evolutionary older vertebrates such as fish. However  how fish cytokines function and whether they have similar roles as in mammals is still a matter of speculation. Here  we have used the zebrafish Danio rerio to gain insights into il4/13a and il4/13b genes and characterized their role under both homeostatic and inflammatory conditions. We established knockouts for both il4/13a and il4/13b genes and showed that they are needed to suppress inflammation in larvae and in the gill mucosa. As a counterpoint  we examined the gills of il10 defective zebrafish and revealed the importance of il10 in maintaining homeostasis in this mucosal tissue. As in mammals  zebrafish il10 appears to have an anti inflammatory function.", "ENA FIRST PUBLIC:2020 05 28|ENA LAST UPDATE:2020 04 16", null, "Protocols: This submission contains RNA seq samples from the gill tissue of five zebrafish strains: Wildtype  il4/13a KO  il4/13b KO  il4/13a;b KO and il10 KO. The strains il4/13a KO  il4/13b KO and il4/13a;b KO were generated using CRISPR/Cas9 system whereas the il10 KO was generated by the Sanger Institute through the Zebrafish Mutation Project Kettleborough RNW  Busch nentwich EM  Harvey SA  Dooley CM  Bruijn E De  Eeden F Van  et al. Europe PMC Funders Group Europe PMC Funders Author Manuscripts A systematic genome wide analysis of zebrafish protein coding gene function. 2013;4967446:494 7. Zebrafish Danio rerio were maintained under standard conditions 28 \u00b0C under a 14 h light/10 h dark cycle within the Biological Services Unit The University of Manchester. Gill tissue was harvested from adult 6 mpf male and female zebrafish. Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen. Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen. Prior to performing RNA seq  the integrity of RNA samples was assessed using a 2200 TapeStation Agilent Technologies according to the manufacturer's instructions. Truseq stranded mRNA Assay. Paired end.", "Sample 15", "SAMEA6786444", "UNIVERSITY OF MANCHESTER", "ENA FIRST PUBLIC:2020 05 28T17:07:47Z|ENA LAST UPDATE:2020 04 16T14:58:15Z|External Id:SAMEA6786444|INSDC center name:UNIVERSITY OF MANCHESTER|INSDC first public:2020 05 28T17:07:47Z|INSDC last update:2020 04 16T14:58:15Z|INSDC status:public|Submitter Id:E MTAB 8958:Sample 15|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:il4/13b  / |organism part:gill|sample name:E MTAB 8958:Sample 15|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Illumina HiSeq 4000 paired end sequencing; Zebrafish il 4 il 10 regulated immunity in gills", "E MTAB 8958:Sample 15 p", "Sample 15 p", "Zebrafish il 4 il 10 regulated immunity in gills", "This submission contains RNA seq samples from the gill tissue of five zebrafish strains: Wildtype  il4/13a KO  il4/13b KO  il4/13a;b KO and il10 KO. The strains il4/13a KO  il4/13b KO and il4/13a;b KO were generated using CRISPR/Cas9 system whereas the  il10 KO was generated by the Sanger Institute through the Zebrafish Mutation Project Kettleborough RNW  Busch nentwich EM  Harvey SA  Dooley CM  Bruijn E De  Eeden F Van  et al. Europe PMC Funders Group Europe PMC Funders Author Manuscripts A systematic genome wide analysis of zebrafish protein coding gene function. 2013;4967446:494\u20137. Zebrafish Danio rerio were maintained under standard conditions 28 \u00b0C under a 14 h light/10 h dark cycle within the Biological Services Unit The University of Manchester. Gill tissue was harvested from adult 6 mpf male and female zebrafish. Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen.  Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen. Prior to performing RNA seq  the integrity of RNA samples was assessed using a 2200 TapeStation Agilent Technologies according to the manufacturer's instructions.  Truseq stranded mRNA Assay. Paired end.", "Experimental Factor: genotype:il4/13b  / ", "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "ERP121190", "Illumina HiSeq 4000 paired end sequencing; Zebrafish il 4 il 10 regulated immunity in gills", "ENA FIRST PUBLIC:2020 05 28|ENA LAST UPDATE:2020 04 16", "FB3_S19_R1_001.fastq.gz FB3_S19_R2_001.fastq.gz", "fastq fastq", 6835575760.0, 45515064.0, "E MTAB 8958:FB3 S19 R", "0:75.11 1:75.07", "A:1802176562;C:1589590146;G:1564279107;T:1858606235;N:20923710", 75, 75, null, null, 1802176562, 1589590146, 1564279107, 1858606235, 20923710, "ERX4030570", "ERS4514123", "ERA2508092", "UNIVERSITY OF MANCHESTER|European Nucleotide Archive", "UNIVERSITY OF MANCHESTER|European Nucleotide Archive", 2, 0.90945, 0.91123, 0.09519, 0.09378, 0.67963, 0.67961, 0.49246, 0.49476, 76, 76, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "trueseq", "bulk", "unknown", "unknown", null, "United Kingdom", "2020-04-16", "Adult", "Adult", "Gill", "Respiratory System"], [9848, "ERR4029253", "ERX4030569", "ERS4514122", "ERP121190", "PRJEB37851", "Zebrafish il 4 il 10 regulated immunity in gills", "E-MTAB-8958", "Transcriptome Analysis", "The evolutionary origin of vertebrate type 2 immunity is a topic of great interest. Several studies have focused on the immune cell components of evolutionary older vertebrates such as fish. However  how fish cytokines function and whether they have similar roles as in mammals is still a matter of speculation. Here  we have used the zebrafish Danio rerio to gain insights into il4/13a and il4/13b genes and characterized their role under both homeostatic and inflammatory conditions. We established knockouts for both il4/13a and il4/13b genes and showed that they are needed to suppress inflammation in larvae and in the gill mucosa. As a counterpoint  we examined the gills of il10 defective zebrafish and revealed the importance of il10 in maintaining homeostasis in this mucosal tissue. As in mammals  zebrafish il10 appears to have an anti inflammatory function.", "ENA FIRST PUBLIC:2020 05 28|ENA LAST UPDATE:2020 04 16", null, "Protocols: This submission contains RNA seq samples from the gill tissue of five zebrafish strains: Wildtype  il4/13a KO  il4/13b KO  il4/13a;b KO and il10 KO. The strains il4/13a KO  il4/13b KO and il4/13a;b KO were generated using CRISPR/Cas9 system whereas the il10 KO was generated by the Sanger Institute through the Zebrafish Mutation Project Kettleborough RNW  Busch nentwich EM  Harvey SA  Dooley CM  Bruijn E De  Eeden F Van  et al. Europe PMC Funders Group Europe PMC Funders Author Manuscripts A systematic genome wide analysis of zebrafish protein coding gene function. 2013;4967446:494 7. Zebrafish Danio rerio were maintained under standard conditions 28 \u00b0C under a 14 h light/10 h dark cycle within the Biological Services Unit The University of Manchester. Gill tissue was harvested from adult 6 mpf male and female zebrafish. Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen. Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen. Prior to performing RNA seq  the integrity of RNA samples was assessed using a 2200 TapeStation Agilent Technologies according to the manufacturer's instructions. Truseq stranded mRNA Assay. Paired end.", "Sample 14", "SAMEA6786443", "UNIVERSITY OF MANCHESTER", "ENA FIRST PUBLIC:2020 05 28T17:07:47Z|ENA LAST UPDATE:2020 04 16T14:58:15Z|External Id:SAMEA6786443|INSDC center name:UNIVERSITY OF MANCHESTER|INSDC first public:2020 05 28T17:07:47Z|INSDC last update:2020 04 16T14:58:15Z|INSDC status:public|Submitter Id:E MTAB 8958:Sample 14|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:il4/13b  / |organism part:gill|sample name:E MTAB 8958:Sample 14|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Illumina HiSeq 4000 paired end sequencing; Zebrafish il 4 il 10 regulated immunity in gills", "E MTAB 8958:Sample 14 p", "Sample 14 p", "Zebrafish il 4 il 10 regulated immunity in gills", "This submission contains RNA seq samples from the gill tissue of five zebrafish strains: Wildtype  il4/13a KO  il4/13b KO  il4/13a;b KO and il10 KO. The strains il4/13a KO  il4/13b KO and il4/13a;b KO were generated using CRISPR/Cas9 system whereas the  il10 KO was generated by the Sanger Institute through the Zebrafish Mutation Project Kettleborough RNW  Busch nentwich EM  Harvey SA  Dooley CM  Bruijn E De  Eeden F Van  et al. Europe PMC Funders Group Europe PMC Funders Author Manuscripts A systematic genome wide analysis of zebrafish protein coding gene function. 2013;4967446:494\u20137. Zebrafish Danio rerio were maintained under standard conditions 28 \u00b0C under a 14 h light/10 h dark cycle within the Biological Services Unit The University of Manchester. Gill tissue was harvested from adult 6 mpf male and female zebrafish. Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen.  Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen. Prior to performing RNA seq  the integrity of RNA samples was assessed using a 2200 TapeStation Agilent Technologies according to the manufacturer's instructions.  Truseq stranded mRNA Assay. Paired end.", "Experimental Factor: genotype:il4/13b  / ", "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "ERP121190", "Illumina HiSeq 4000 paired end sequencing; Zebrafish il 4 il 10 regulated immunity in gills", "ENA FIRST PUBLIC:2020 05 28|ENA LAST UPDATE:2020 04 16", "FB2_S18_R1_001.fastq.gz FB2_S18_R2_001.fastq.gz", "fastq fastq", 5789923311.0, 38517674.0, "E MTAB 8958:FB2 S18 R", "0:75.18 1:75.14", "A:1520250885;C:1354960735;G:1336204150;T:1565011084;N:13496457", 75, 75, null, null, 1520250885, 1354960735, 1336204150, 1565011084, 13496457, "ERX4030569", "ERS4514122", "ERA2508092", "UNIVERSITY OF MANCHESTER|European Nucleotide Archive", "UNIVERSITY OF MANCHESTER|European Nucleotide Archive", 2, 0.91863, 0.91917, 0.08923, 0.08753, 0.68848, 0.68883, 0.5042, 0.50799, 76, 76, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "trueseq", "bulk", "unknown", "unknown", null, "United Kingdom", "2020-04-16", "Adult", "Adult", "Gill", "Respiratory System"], [9849, "ERR4029252", "ERX4030568", "ERS4514121", "ERP121190", "PRJEB37851", "Zebrafish il 4 il 10 regulated immunity in gills", "E-MTAB-8958", "Transcriptome Analysis", "The evolutionary origin of vertebrate type 2 immunity is a topic of great interest. Several studies have focused on the immune cell components of evolutionary older vertebrates such as fish. However  how fish cytokines function and whether they have similar roles as in mammals is still a matter of speculation. Here  we have used the zebrafish Danio rerio to gain insights into il4/13a and il4/13b genes and characterized their role under both homeostatic and inflammatory conditions. We established knockouts for both il4/13a and il4/13b genes and showed that they are needed to suppress inflammation in larvae and in the gill mucosa. As a counterpoint  we examined the gills of il10 defective zebrafish and revealed the importance of il10 in maintaining homeostasis in this mucosal tissue. As in mammals  zebrafish il10 appears to have an anti inflammatory function.", "ENA FIRST PUBLIC:2020 05 28|ENA LAST UPDATE:2020 04 16", null, "Protocols: This submission contains RNA seq samples from the gill tissue of five zebrafish strains: Wildtype  il4/13a KO  il4/13b KO  il4/13a;b KO and il10 KO. The strains il4/13a KO  il4/13b KO and il4/13a;b KO were generated using CRISPR/Cas9 system whereas the il10 KO was generated by the Sanger Institute through the Zebrafish Mutation Project Kettleborough RNW  Busch nentwich EM  Harvey SA  Dooley CM  Bruijn E De  Eeden F Van  et al. Europe PMC Funders Group Europe PMC Funders Author Manuscripts A systematic genome wide analysis of zebrafish protein coding gene function. 2013;4967446:494 7. Zebrafish Danio rerio were maintained under standard conditions 28 \u00b0C under a 14 h light/10 h dark cycle within the Biological Services Unit The University of Manchester. Gill tissue was harvested from adult 6 mpf male and female zebrafish. Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen. Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen. Prior to performing RNA seq  the integrity of RNA samples was assessed using a 2200 TapeStation Agilent Technologies according to the manufacturer's instructions. Truseq stranded mRNA Assay. Paired end.", "Sample 13", "SAMEA6786442", "UNIVERSITY OF MANCHESTER", "ENA FIRST PUBLIC:2020 05 28T17:07:47Z|ENA LAST UPDATE:2020 04 16T14:58:15Z|External Id:SAMEA6786442|INSDC center name:UNIVERSITY OF MANCHESTER|INSDC first public:2020 05 28T17:07:47Z|INSDC last update:2020 04 16T14:58:15Z|INSDC status:public|Submitter Id:E MTAB 8958:Sample 13|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:il4/13b  / |organism part:gill|sample name:E MTAB 8958:Sample 13|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Illumina HiSeq 4000 paired end sequencing; Zebrafish il 4 il 10 regulated immunity in gills", "E MTAB 8958:Sample 13 p", "Sample 13 p", "Zebrafish il 4 il 10 regulated immunity in gills", "This submission contains RNA seq samples from the gill tissue of five zebrafish strains: Wildtype  il4/13a KO  il4/13b KO  il4/13a;b KO and il10 KO. The strains il4/13a KO  il4/13b KO and il4/13a;b KO were generated using CRISPR/Cas9 system whereas the  il10 KO was generated by the Sanger Institute through the Zebrafish Mutation Project Kettleborough RNW  Busch nentwich EM  Harvey SA  Dooley CM  Bruijn E De  Eeden F Van  et al. Europe PMC Funders Group Europe PMC Funders Author Manuscripts A systematic genome wide analysis of zebrafish protein coding gene function. 2013;4967446:494\u20137. Zebrafish Danio rerio were maintained under standard conditions 28 \u00b0C under a 14 h light/10 h dark cycle within the Biological Services Unit The University of Manchester. Gill tissue was harvested from adult 6 mpf male and female zebrafish. Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen.  Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen. Prior to performing RNA seq  the integrity of RNA samples was assessed using a 2200 TapeStation Agilent Technologies according to the manufacturer's instructions.  Truseq stranded mRNA Assay. Paired end.", "Experimental Factor: genotype:il4/13b  / ", "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "ERP121190", "Illumina HiSeq 4000 paired end sequencing; Zebrafish il 4 il 10 regulated immunity in gills", "ENA FIRST PUBLIC:2020 05 28|ENA LAST UPDATE:2020 04 16", "FB1_S17_R1_001.fastq.gz FB1_S17_R2_001.fastq.gz", "fastq fastq", 6951804793.0, 46295025.0, "E MTAB 8958:FB1 S17 R", "0:75.10 1:75.06", "A:1827618209;C:1618955946;G:1595802123;T:1884739421;N:24689094", 75, 75, null, null, 1827618209, 1618955946, 1595802123, 1884739421, 24689094, "ERX4030568", "ERS4514121", "ERA2508092", "UNIVERSITY OF MANCHESTER|European Nucleotide Archive", "UNIVERSITY OF MANCHESTER|European Nucleotide Archive", 2, 0.90991, 0.90972, 0.09297, 0.09193, 0.68284, 0.68392, 0.49878, 0.5017, 74, 75, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "trueseq", "bulk", "unknown", "unknown", null, "United Kingdom", "2020-04-16", "Adult", "Adult", "Gill", "Respiratory System"], [9850, "ERR4029251", "ERX4030567", "ERS4514120", "ERP121190", "PRJEB37851", "Zebrafish il 4 il 10 regulated immunity in gills", "E-MTAB-8958", "Transcriptome Analysis", "The evolutionary origin of vertebrate type 2 immunity is a topic of great interest. Several studies have focused on the immune cell components of evolutionary older vertebrates such as fish. However  how fish cytokines function and whether they have similar roles as in mammals is still a matter of speculation. Here  we have used the zebrafish Danio rerio to gain insights into il4/13a and il4/13b genes and characterized their role under both homeostatic and inflammatory conditions. We established knockouts for both il4/13a and il4/13b genes and showed that they are needed to suppress inflammation in larvae and in the gill mucosa. As a counterpoint  we examined the gills of il10 defective zebrafish and revealed the importance of il10 in maintaining homeostasis in this mucosal tissue. As in mammals  zebrafish il10 appears to have an anti inflammatory function.", "ENA FIRST PUBLIC:2020 05 28|ENA LAST UPDATE:2020 04 16", null, "Protocols: This submission contains RNA seq samples from the gill tissue of five zebrafish strains: Wildtype  il4/13a KO  il4/13b KO  il4/13a;b KO and il10 KO. The strains il4/13a KO  il4/13b KO and il4/13a;b KO were generated using CRISPR/Cas9 system whereas the il10 KO was generated by the Sanger Institute through the Zebrafish Mutation Project Kettleborough RNW  Busch nentwich EM  Harvey SA  Dooley CM  Bruijn E De  Eeden F Van  et al. Europe PMC Funders Group Europe PMC Funders Author Manuscripts A systematic genome wide analysis of zebrafish protein coding gene function. 2013;4967446:494 7. Zebrafish Danio rerio were maintained under standard conditions 28 \u00b0C under a 14 h light/10 h dark cycle within the Biological Services Unit The University of Manchester. Gill tissue was harvested from adult 6 mpf male and female zebrafish. Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen. Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen. Prior to performing RNA seq  the integrity of RNA samples was assessed using a 2200 TapeStation Agilent Technologies according to the manufacturer's instructions. Truseq stranded mRNA Assay. Paired end.", "Sample 20", "SAMEA6786441", "UNIVERSITY OF MANCHESTER", "ENA FIRST PUBLIC:2020 05 28T17:07:47Z|ENA LAST UPDATE:2020 04 16T14:58:15Z|External Id:SAMEA6786441|INSDC center name:UNIVERSITY OF MANCHESTER|INSDC first public:2020 05 28T17:07:47Z|INSDC last update:2020 04 16T14:58:15Z|INSDC status:public|Submitter Id:E MTAB 8958:Sample 20|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:il4/13a  / ; il4/13b  / |organism part:gill|sample name:E MTAB 8958:Sample 20|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Illumina HiSeq 4000 paired end sequencing; Zebrafish il 4 il 10 regulated immunity in gills", "E MTAB 8958:Sample 20 p", "Sample 20 p", "Zebrafish il 4 il 10 regulated immunity in gills", "This submission contains RNA seq samples from the gill tissue of five zebrafish strains: Wildtype  il4/13a KO  il4/13b KO  il4/13a;b KO and il10 KO. The strains il4/13a KO  il4/13b KO and il4/13a;b KO were generated using CRISPR/Cas9 system whereas the  il10 KO was generated by the Sanger Institute through the Zebrafish Mutation Project Kettleborough RNW  Busch nentwich EM  Harvey SA  Dooley CM  Bruijn E De  Eeden F Van  et al. Europe PMC Funders Group Europe PMC Funders Author Manuscripts A systematic genome wide analysis of zebrafish protein coding gene function. 2013;4967446:494\u20137. Zebrafish Danio rerio were maintained under standard conditions 28 \u00b0C under a 14 h light/10 h dark cycle within the Biological Services Unit The University of Manchester. Gill tissue was harvested from adult 6 mpf male and female zebrafish. Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen.  Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen. Prior to performing RNA seq  the integrity of RNA samples was assessed using a 2200 TapeStation Agilent Technologies according to the manufacturer's instructions.  Truseq stranded mRNA Assay. Paired end.", "Experimental Factor: genotype:il4/13a   / ; il4/13b  / ", "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "ERP121190", "Illumina HiSeq 4000 paired end sequencing; Zebrafish il 4 il 10 regulated immunity in gills", "ENA FIRST PUBLIC:2020 05 28|ENA LAST UPDATE:2020 04 16", "FB4_S44_R1_001.fastq.gz FB4_S44_R2_001.fastq.gz", "fastq fastq", 9629684100.0, 64667395.0, "E MTAB 8958:FB4 S44 R", "0:74.47 1:74.44", "A:2552333346;C:2176006333;G:2180111416;T:2614191329;N:107041676", 74, 74, null, null, 2552333346, 2176006333, 2180111416, 2614191329, 107041676, "ERX4030567", "ERS4514120", "ERA2508092", "UNIVERSITY OF MANCHESTER|European Nucleotide Archive", "UNIVERSITY OF MANCHESTER|European Nucleotide Archive", 2, 0.90795, 0.90756, 0.10044, 0.09789, 0.6888, 0.69256, 0.50043, 0.4932, 76, 76, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "trueseq", "bulk", "unknown", "unknown", null, "United Kingdom", "2020-04-16", "Adult", "Adult", "Gill", "Respiratory System"], [9851, "ERR4029250", "ERX4030566", "ERS4514119", "ERP121190", "PRJEB37851", "Zebrafish il 4 il 10 regulated immunity in gills", "E-MTAB-8958", "Transcriptome Analysis", "The evolutionary origin of vertebrate type 2 immunity is a topic of great interest. Several studies have focused on the immune cell components of evolutionary older vertebrates such as fish. However  how fish cytokines function and whether they have similar roles as in mammals is still a matter of speculation. Here  we have used the zebrafish Danio rerio to gain insights into il4/13a and il4/13b genes and characterized their role under both homeostatic and inflammatory conditions. We established knockouts for both il4/13a and il4/13b genes and showed that they are needed to suppress inflammation in larvae and in the gill mucosa. As a counterpoint  we examined the gills of il10 defective zebrafish and revealed the importance of il10 in maintaining homeostasis in this mucosal tissue. As in mammals  zebrafish il10 appears to have an anti inflammatory function.", "ENA FIRST PUBLIC:2020 05 28|ENA LAST UPDATE:2020 04 16", null, "Protocols: This submission contains RNA seq samples from the gill tissue of five zebrafish strains: Wildtype  il4/13a KO  il4/13b KO  il4/13a;b KO and il10 KO. The strains il4/13a KO  il4/13b KO and il4/13a;b KO were generated using CRISPR/Cas9 system whereas the il10 KO was generated by the Sanger Institute through the Zebrafish Mutation Project Kettleborough RNW  Busch nentwich EM  Harvey SA  Dooley CM  Bruijn E De  Eeden F Van  et al. Europe PMC Funders Group Europe PMC Funders Author Manuscripts A systematic genome wide analysis of zebrafish protein coding gene function. 2013;4967446:494 7. Zebrafish Danio rerio were maintained under standard conditions 28 \u00b0C under a 14 h light/10 h dark cycle within the Biological Services Unit The University of Manchester. Gill tissue was harvested from adult 6 mpf male and female zebrafish. Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen. Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen. Prior to performing RNA seq  the integrity of RNA samples was assessed using a 2200 TapeStation Agilent Technologies according to the manufacturer's instructions. Truseq stranded mRNA Assay. Paired end.", "Sample 19", "SAMEA6786440", "UNIVERSITY OF MANCHESTER", "ENA FIRST PUBLIC:2020 05 28T17:07:47Z|ENA LAST UPDATE:2020 04 16T14:58:15Z|External Id:SAMEA6786440|INSDC center name:UNIVERSITY OF MANCHESTER|INSDC first public:2020 05 28T17:07:47Z|INSDC last update:2020 04 16T14:58:15Z|INSDC status:public|Submitter Id:E MTAB 8958:Sample 19|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:il4/13a  / ; il4/13b  / |organism part:gill|sample name:E MTAB 8958:Sample 19|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Illumina HiSeq 4000 paired end sequencing; Zebrafish il 4 il 10 regulated immunity in gills", "E MTAB 8958:Sample 19 p", "Sample 19 p", "Zebrafish il 4 il 10 regulated immunity in gills", "This submission contains RNA seq samples from the gill tissue of five zebrafish strains: Wildtype  il4/13a KO  il4/13b KO  il4/13a;b KO and il10 KO. The strains il4/13a KO  il4/13b KO and il4/13a;b KO were generated using CRISPR/Cas9 system whereas the  il10 KO was generated by the Sanger Institute through the Zebrafish Mutation Project Kettleborough RNW  Busch nentwich EM  Harvey SA  Dooley CM  Bruijn E De  Eeden F Van  et al. Europe PMC Funders Group Europe PMC Funders Author Manuscripts A systematic genome wide analysis of zebrafish protein coding gene function. 2013;4967446:494\u20137. Zebrafish Danio rerio were maintained under standard conditions 28 \u00b0C under a 14 h light/10 h dark cycle within the Biological Services Unit The University of Manchester. Gill tissue was harvested from adult 6 mpf male and female zebrafish. Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen.  Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen. Prior to performing RNA seq  the integrity of RNA samples was assessed using a 2200 TapeStation Agilent Technologies according to the manufacturer's instructions.  Truseq stranded mRNA Assay. Paired end.", "Experimental Factor: genotype:il4/13a   / ; il4/13b  / ", "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "ERP121190", "Illumina HiSeq 4000 paired end sequencing; Zebrafish il 4 il 10 regulated immunity in gills", "ENA FIRST PUBLIC:2020 05 28|ENA LAST UPDATE:2020 04 16", "FB3_S43_R1_001.fastq.gz FB3_S43_R2_001.fastq.gz", "fastq fastq", 11268744444.0, 75088756.0, "E MTAB 8958:FB3 S43 R", "0:75.06 1:75.01", "A:2983863814;C:2608268633;G:2555098938;T:3072413168;N:49099891", 75, 75, null, null, 2983863814, 2608268633, 2555098938, 3072413168, 49099891, "ERX4030566", "ERS4514119", "ERA2508092", "UNIVERSITY OF MANCHESTER|European Nucleotide Archive", "UNIVERSITY OF MANCHESTER|European Nucleotide Archive", 2, 0.9107, 0.91089, 0.09522, 0.09428, 0.69187, 0.69266, 0.49302, 0.49463, 76, 76, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "trueseq", "bulk", "unknown", "unknown", null, "United Kingdom", "2020-04-16", "Adult", "Adult", "Gill", "Respiratory System"], [9852, "ERR4029249", "ERX4030565", "ERS4514118", "ERP121190", "PRJEB37851", "Zebrafish il 4 il 10 regulated immunity in gills", "E-MTAB-8958", "Transcriptome Analysis", "The evolutionary origin of vertebrate type 2 immunity is a topic of great interest. Several studies have focused on the immune cell components of evolutionary older vertebrates such as fish. However  how fish cytokines function and whether they have similar roles as in mammals is still a matter of speculation. Here  we have used the zebrafish Danio rerio to gain insights into il4/13a and il4/13b genes and characterized their role under both homeostatic and inflammatory conditions. We established knockouts for both il4/13a and il4/13b genes and showed that they are needed to suppress inflammation in larvae and in the gill mucosa. As a counterpoint  we examined the gills of il10 defective zebrafish and revealed the importance of il10 in maintaining homeostasis in this mucosal tissue. As in mammals  zebrafish il10 appears to have an anti inflammatory function.", "ENA FIRST PUBLIC:2020 05 28|ENA LAST UPDATE:2020 04 16", null, "Protocols: This submission contains RNA seq samples from the gill tissue of five zebrafish strains: Wildtype  il4/13a KO  il4/13b KO  il4/13a;b KO and il10 KO. The strains il4/13a KO  il4/13b KO and il4/13a;b KO were generated using CRISPR/Cas9 system whereas the il10 KO was generated by the Sanger Institute through the Zebrafish Mutation Project Kettleborough RNW  Busch nentwich EM  Harvey SA  Dooley CM  Bruijn E De  Eeden F Van  et al. Europe PMC Funders Group Europe PMC Funders Author Manuscripts A systematic genome wide analysis of zebrafish protein coding gene function. 2013;4967446:494 7. Zebrafish Danio rerio were maintained under standard conditions 28 \u00b0C under a 14 h light/10 h dark cycle within the Biological Services Unit The University of Manchester. Gill tissue was harvested from adult 6 mpf male and female zebrafish. Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen. Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen. Prior to performing RNA seq  the integrity of RNA samples was assessed using a 2200 TapeStation Agilent Technologies according to the manufacturer's instructions. Truseq stranded mRNA Assay. Paired end.", "Sample 18", "SAMEA6786439", "UNIVERSITY OF MANCHESTER", "ENA FIRST PUBLIC:2020 05 28T17:07:47Z|ENA LAST UPDATE:2020 04 16T14:58:14Z|External Id:SAMEA6786439|INSDC center name:UNIVERSITY OF MANCHESTER|INSDC first public:2020 05 28T17:07:47Z|INSDC last update:2020 04 16T14:58:14Z|INSDC status:public|Submitter Id:E MTAB 8958:Sample 18|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:il4/13a  / ; il4/13b  / |organism part:gill|sample name:E MTAB 8958:Sample 18|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Illumina HiSeq 4000 paired end sequencing; Zebrafish il 4 il 10 regulated immunity in gills", "E MTAB 8958:Sample 18 p", "Sample 18 p", "Zebrafish il 4 il 10 regulated immunity in gills", "This submission contains RNA seq samples from the gill tissue of five zebrafish strains: Wildtype  il4/13a KO  il4/13b KO  il4/13a;b KO and il10 KO. The strains il4/13a KO  il4/13b KO and il4/13a;b KO were generated using CRISPR/Cas9 system whereas the  il10 KO was generated by the Sanger Institute through the Zebrafish Mutation Project Kettleborough RNW  Busch nentwich EM  Harvey SA  Dooley CM  Bruijn E De  Eeden F Van  et al. Europe PMC Funders Group Europe PMC Funders Author Manuscripts A systematic genome wide analysis of zebrafish protein coding gene function. 2013;4967446:494\u20137. Zebrafish Danio rerio were maintained under standard conditions 28 \u00b0C under a 14 h light/10 h dark cycle within the Biological Services Unit The University of Manchester. Gill tissue was harvested from adult 6 mpf male and female zebrafish. Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen.  Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen. Prior to performing RNA seq  the integrity of RNA samples was assessed using a 2200 TapeStation Agilent Technologies according to the manufacturer's instructions.  Truseq stranded mRNA Assay. Paired end.", "Experimental Factor: genotype:il4/13a   / ; il4/13b  / ", "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "ERP121190", "Illumina HiSeq 4000 paired end sequencing; Zebrafish il 4 il 10 regulated immunity in gills", "ENA FIRST PUBLIC:2020 05 28|ENA LAST UPDATE:2020 04 16", "FB2_S42_R1_001.fastq.gz FB2_S42_R2_001.fastq.gz", "fastq fastq", 10434072067.0, 69530304.0, "E MTAB 8958:FB2 S42 R", "0:75.06 1:75.01", "A:2749500618;C:2426208950;G:2375421255;T:2834879202;N:48062042", 75, 75, null, null, 2749500618, 2426208950, 2375421255, 2834879202, 48062042, "ERX4030565", "ERS4514118", "ERA2508092", "UNIVERSITY OF MANCHESTER|European Nucleotide Archive", "UNIVERSITY OF MANCHESTER|European Nucleotide Archive", 2, 0.9084, 0.90779, 0.0926, 0.0917, 0.68809, 0.68864, 0.49817, 0.49902, 76, 75, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "trueseq", "bulk", "unknown", "unknown", null, "United Kingdom", "2020-04-16", "Adult", "Adult", "Gill", "Respiratory System"], [9853, "ERR4029248", "ERX4030564", "ERS4514117", "ERP121190", "PRJEB37851", "Zebrafish il 4 il 10 regulated immunity in gills", "E-MTAB-8958", "Transcriptome Analysis", "The evolutionary origin of vertebrate type 2 immunity is a topic of great interest. Several studies have focused on the immune cell components of evolutionary older vertebrates such as fish. However  how fish cytokines function and whether they have similar roles as in mammals is still a matter of speculation. Here  we have used the zebrafish Danio rerio to gain insights into il4/13a and il4/13b genes and characterized their role under both homeostatic and inflammatory conditions. We established knockouts for both il4/13a and il4/13b genes and showed that they are needed to suppress inflammation in larvae and in the gill mucosa. As a counterpoint  we examined the gills of il10 defective zebrafish and revealed the importance of il10 in maintaining homeostasis in this mucosal tissue. As in mammals  zebrafish il10 appears to have an anti inflammatory function.", "ENA FIRST PUBLIC:2020 05 28|ENA LAST UPDATE:2020 04 16", null, "Protocols: This submission contains RNA seq samples from the gill tissue of five zebrafish strains: Wildtype  il4/13a KO  il4/13b KO  il4/13a;b KO and il10 KO. The strains il4/13a KO  il4/13b KO and il4/13a;b KO were generated using CRISPR/Cas9 system whereas the il10 KO was generated by the Sanger Institute through the Zebrafish Mutation Project Kettleborough RNW  Busch nentwich EM  Harvey SA  Dooley CM  Bruijn E De  Eeden F Van  et al. Europe PMC Funders Group Europe PMC Funders Author Manuscripts A systematic genome wide analysis of zebrafish protein coding gene function. 2013;4967446:494 7. Zebrafish Danio rerio were maintained under standard conditions 28 \u00b0C under a 14 h light/10 h dark cycle within the Biological Services Unit The University of Manchester. Gill tissue was harvested from adult 6 mpf male and female zebrafish. Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen. Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen. Prior to performing RNA seq  the integrity of RNA samples was assessed using a 2200 TapeStation Agilent Technologies according to the manufacturer's instructions. Truseq stranded mRNA Assay. Paired end.", "Sample 17", "SAMEA6786438", "UNIVERSITY OF MANCHESTER", "ENA FIRST PUBLIC:2020 05 28T17:07:47Z|ENA LAST UPDATE:2020 04 16T14:58:14Z|External Id:SAMEA6786438|INSDC center name:UNIVERSITY OF MANCHESTER|INSDC first public:2020 05 28T17:07:47Z|INSDC last update:2020 04 16T14:58:14Z|INSDC status:public|Submitter Id:E MTAB 8958:Sample 17|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:il4/13a  / ; il4/13b  / |organism part:gill|sample name:E MTAB 8958:Sample 17|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Illumina HiSeq 4000 paired end sequencing; Zebrafish il 4 il 10 regulated immunity in gills", "E MTAB 8958:Sample 17 p", "Sample 17 p", "Zebrafish il 4 il 10 regulated immunity in gills", "This submission contains RNA seq samples from the gill tissue of five zebrafish strains: Wildtype  il4/13a KO  il4/13b KO  il4/13a;b KO and il10 KO. The strains il4/13a KO  il4/13b KO and il4/13a;b KO were generated using CRISPR/Cas9 system whereas the  il10 KO was generated by the Sanger Institute through the Zebrafish Mutation Project Kettleborough RNW  Busch nentwich EM  Harvey SA  Dooley CM  Bruijn E De  Eeden F Van  et al. Europe PMC Funders Group Europe PMC Funders Author Manuscripts A systematic genome wide analysis of zebrafish protein coding gene function. 2013;4967446:494\u20137. Zebrafish Danio rerio were maintained under standard conditions 28 \u00b0C under a 14 h light/10 h dark cycle within the Biological Services Unit The University of Manchester. Gill tissue was harvested from adult 6 mpf male and female zebrafish. Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen.  Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen. Prior to performing RNA seq  the integrity of RNA samples was assessed using a 2200 TapeStation Agilent Technologies according to the manufacturer's instructions.  Truseq stranded mRNA Assay. Paired end.", "Experimental Factor: genotype:il4/13a   / ; il4/13b  / ", "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "ERP121190", "Illumina HiSeq 4000 paired end sequencing; Zebrafish il 4 il 10 regulated immunity in gills", "ENA FIRST PUBLIC:2020 05 28|ENA LAST UPDATE:2020 04 16", "FB1_S41_R1_001.fastq.gz FB1_S41_R2_001.fastq.gz", "fastq fastq", 12125040750.0, 80781224.0, "E MTAB 8958:FB1 S41 R", "0:75.08 1:75.02", "A:3129480022;C:2890038396;G:2819859086;T:3233228646;N:52434600", 75, 75, null, null, 3129480022, 2890038396, 2819859086, 3233228646, 52434600, "ERX4030564", "ERS4514117", "ERA2508092", "UNIVERSITY OF MANCHESTER|European Nucleotide Archive", "UNIVERSITY OF MANCHESTER|European Nucleotide Archive", 2, 0.91384, 0.91301, 0.10967, 0.10615, 0.69006, 0.69041, 0.51643, 0.51985, 76, 76, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "trueseq", "bulk", "unknown", "unknown", null, "United Kingdom", "2020-04-16", "Adult", "Adult", "Gill", "Respiratory System"], [9854, "ERR4029247", "ERX4030563", "ERS4514116", "ERP121190", "PRJEB37851", "Zebrafish il 4 il 10 regulated immunity in gills", "E-MTAB-8958", "Transcriptome Analysis", "The evolutionary origin of vertebrate type 2 immunity is a topic of great interest. Several studies have focused on the immune cell components of evolutionary older vertebrates such as fish. However  how fish cytokines function and whether they have similar roles as in mammals is still a matter of speculation. Here  we have used the zebrafish Danio rerio to gain insights into il4/13a and il4/13b genes and characterized their role under both homeostatic and inflammatory conditions. We established knockouts for both il4/13a and il4/13b genes and showed that they are needed to suppress inflammation in larvae and in the gill mucosa. As a counterpoint  we examined the gills of il10 defective zebrafish and revealed the importance of il10 in maintaining homeostasis in this mucosal tissue. As in mammals  zebrafish il10 appears to have an anti inflammatory function.", "ENA FIRST PUBLIC:2020 05 28|ENA LAST UPDATE:2020 04 16", null, "Protocols: This submission contains RNA seq samples from the gill tissue of five zebrafish strains: Wildtype  il4/13a KO  il4/13b KO  il4/13a;b KO and il10 KO. The strains il4/13a KO  il4/13b KO and il4/13a;b KO were generated using CRISPR/Cas9 system whereas the il10 KO was generated by the Sanger Institute through the Zebrafish Mutation Project Kettleborough RNW  Busch nentwich EM  Harvey SA  Dooley CM  Bruijn E De  Eeden F Van  et al. Europe PMC Funders Group Europe PMC Funders Author Manuscripts A systematic genome wide analysis of zebrafish protein coding gene function. 2013;4967446:494 7. Zebrafish Danio rerio were maintained under standard conditions 28 \u00b0C under a 14 h light/10 h dark cycle within the Biological Services Unit The University of Manchester. Gill tissue was harvested from adult 6 mpf male and female zebrafish. Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen. Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen. Prior to performing RNA seq  the integrity of RNA samples was assessed using a 2200 TapeStation Agilent Technologies according to the manufacturer's instructions. Truseq stranded mRNA Assay. Paired end.", "Sample 8", "SAMEA6786437", "UNIVERSITY OF MANCHESTER", "ENA FIRST PUBLIC:2020 05 28T17:07:47Z|ENA LAST UPDATE:2020 04 16T14:58:14Z|External Id:SAMEA6786437|INSDC center name:UNIVERSITY OF MANCHESTER|INSDC first public:2020 05 28T17:07:47Z|INSDC last update:2020 04 16T14:58:14Z|INSDC status:public|Submitter Id:E MTAB 8958:Sample 8|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:il4/13a  / |organism part:gill|sample name:E MTAB 8958:Sample 8|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Illumina HiSeq 4000 paired end sequencing; Zebrafish il 4 il 10 regulated immunity in gills", "E MTAB 8958:Sample 8 p", "Sample 8 p", "Zebrafish il 4 il 10 regulated immunity in gills", "This submission contains RNA seq samples from the gill tissue of five zebrafish strains: Wildtype  il4/13a KO  il4/13b KO  il4/13a;b KO and il10 KO. The strains il4/13a KO  il4/13b KO and il4/13a;b KO were generated using CRISPR/Cas9 system whereas the  il10 KO was generated by the Sanger Institute through the Zebrafish Mutation Project Kettleborough RNW  Busch nentwich EM  Harvey SA  Dooley CM  Bruijn E De  Eeden F Van  et al. Europe PMC Funders Group Europe PMC Funders Author Manuscripts A systematic genome wide analysis of zebrafish protein coding gene function. 2013;4967446:494\u20137. Zebrafish Danio rerio were maintained under standard conditions 28 \u00b0C under a 14 h light/10 h dark cycle within the Biological Services Unit The University of Manchester. Gill tissue was harvested from adult 6 mpf male and female zebrafish. Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen.  Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen. Prior to performing RNA seq  the integrity of RNA samples was assessed using a 2200 TapeStation Agilent Technologies according to the manufacturer's instructions.  Truseq stranded mRNA Assay. Paired end.", "Experimental Factor: genotype:il4/13a  / ", "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "ERP121190", "Illumina HiSeq 4000 paired end sequencing; Zebrafish il 4 il 10 regulated immunity in gills", "ENA FIRST PUBLIC:2020 05 28|ENA LAST UPDATE:2020 04 16", "FB12_S49_R1_001.fastq.gz FB12_S49_R2_001.fastq.gz", "fastq fastq", 4223131851.0, 28165494.0, "E MTAB 8958:FB12 S49 R", "0:74.95 1:74.99", "A:1121514270;C:962554300;G:967294488;T:1146544647;N:25224146", 74, 74, null, null, 1121514270, 962554300, 967294488, 1146544647, 25224146, "ERX4030563", "ERS4514116", "ERA2508092", "UNIVERSITY OF MANCHESTER|European Nucleotide Archive", "UNIVERSITY OF MANCHESTER|European Nucleotide Archive", 2, 0.91639, 0.91413, 0.10604, 0.10326, 0.68162, 0.68458, 0.49921, 0.50172, 76, 75, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "trueseq", "bulk", "unknown", "unknown", null, "United Kingdom", "2020-04-16", "Adult", "Adult", "Gill", "Respiratory System"], [9855, "ERR4029246", "ERX4030562", "ERS4514115", "ERP121190", "PRJEB37851", "Zebrafish il 4 il 10 regulated immunity in gills", "E-MTAB-8958", "Transcriptome Analysis", "The evolutionary origin of vertebrate type 2 immunity is a topic of great interest. Several studies have focused on the immune cell components of evolutionary older vertebrates such as fish. However  how fish cytokines function and whether they have similar roles as in mammals is still a matter of speculation. Here  we have used the zebrafish Danio rerio to gain insights into il4/13a and il4/13b genes and characterized their role under both homeostatic and inflammatory conditions. We established knockouts for both il4/13a and il4/13b genes and showed that they are needed to suppress inflammation in larvae and in the gill mucosa. As a counterpoint  we examined the gills of il10 defective zebrafish and revealed the importance of il10 in maintaining homeostasis in this mucosal tissue. As in mammals  zebrafish il10 appears to have an anti inflammatory function.", "ENA FIRST PUBLIC:2020 05 28|ENA LAST UPDATE:2020 04 16", null, "Protocols: This submission contains RNA seq samples from the gill tissue of five zebrafish strains: Wildtype  il4/13a KO  il4/13b KO  il4/13a;b KO and il10 KO. The strains il4/13a KO  il4/13b KO and il4/13a;b KO were generated using CRISPR/Cas9 system whereas the il10 KO was generated by the Sanger Institute through the Zebrafish Mutation Project Kettleborough RNW  Busch nentwich EM  Harvey SA  Dooley CM  Bruijn E De  Eeden F Van  et al. Europe PMC Funders Group Europe PMC Funders Author Manuscripts A systematic genome wide analysis of zebrafish protein coding gene function. 2013;4967446:494 7. Zebrafish Danio rerio were maintained under standard conditions 28 \u00b0C under a 14 h light/10 h dark cycle within the Biological Services Unit The University of Manchester. Gill tissue was harvested from adult 6 mpf male and female zebrafish. Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen. Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen. Prior to performing RNA seq  the integrity of RNA samples was assessed using a 2200 TapeStation Agilent Technologies according to the manufacturer's instructions. Truseq stranded mRNA Assay. Paired end.", "Sample 7", "SAMEA6786436", "UNIVERSITY OF MANCHESTER", "ENA FIRST PUBLIC:2020 05 28T17:07:47Z|ENA LAST UPDATE:2020 04 16T14:58:14Z|External Id:SAMEA6786436|INSDC center name:UNIVERSITY OF MANCHESTER|INSDC first public:2020 05 28T17:07:47Z|INSDC last update:2020 04 16T14:58:14Z|INSDC status:public|Submitter Id:E MTAB 8958:Sample 7|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:il4/13a  / |organism part:gill|sample name:E MTAB 8958:Sample 7|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Illumina HiSeq 4000 paired end sequencing; Zebrafish il 4 il 10 regulated immunity in gills", "E MTAB 8958:Sample 7 p", "Sample 7 p", "Zebrafish il 4 il 10 regulated immunity in gills", "This submission contains RNA seq samples from the gill tissue of five zebrafish strains: Wildtype  il4/13a KO  il4/13b KO  il4/13a;b KO and il10 KO. The strains il4/13a KO  il4/13b KO and il4/13a;b KO were generated using CRISPR/Cas9 system whereas the  il10 KO was generated by the Sanger Institute through the Zebrafish Mutation Project Kettleborough RNW  Busch nentwich EM  Harvey SA  Dooley CM  Bruijn E De  Eeden F Van  et al. Europe PMC Funders Group Europe PMC Funders Author Manuscripts A systematic genome wide analysis of zebrafish protein coding gene function. 2013;4967446:494\u20137. Zebrafish Danio rerio were maintained under standard conditions 28 \u00b0C under a 14 h light/10 h dark cycle within the Biological Services Unit The University of Manchester. Gill tissue was harvested from adult 6 mpf male and female zebrafish. Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen.  Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen. Prior to performing RNA seq  the integrity of RNA samples was assessed using a 2200 TapeStation Agilent Technologies according to the manufacturer's instructions.  Truseq stranded mRNA Assay. Paired end.", "Experimental Factor: genotype:il4/13a  / ", "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "ERP121190", "Illumina HiSeq 4000 paired end sequencing; Zebrafish il 4 il 10 regulated immunity in gills", "ENA FIRST PUBLIC:2020 05 28|ENA LAST UPDATE:2020 04 16", "FB11_S48_R1_001.fastq.gz FB11_S48_R2_001.fastq.gz", "fastq fastq", 4670407305.0, 31054128.0, "E MTAB 8958:FB11 S48 R", "0:75.20 1:75.19", "A:1234494738;C:1078360233;G:1084251975;T:1258088405;N:15211954", 75, 75, null, null, 1234494738, 1078360233, 1084251975, 1258088405, 15211954, "ERX4030562", "ERS4514115", "ERA2508092", "UNIVERSITY OF MANCHESTER|European Nucleotide Archive", "UNIVERSITY OF MANCHESTER|European Nucleotide Archive", 2, 0.92228, 0.92038, 0.09418, 0.09222, 0.68674, 0.68862, 0.48948, 0.48842, 75, 75, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "trueseq", "bulk", "unknown", "unknown", null, "United Kingdom", "2020-04-16", "Adult", "Adult", "Gill", "Respiratory System"], [9856, "ERR4029245", "ERX4030561", "ERS4514114", "ERP121190", "PRJEB37851", "Zebrafish il 4 il 10 regulated immunity in gills", "E-MTAB-8958", "Transcriptome Analysis", "The evolutionary origin of vertebrate type 2 immunity is a topic of great interest. Several studies have focused on the immune cell components of evolutionary older vertebrates such as fish. However  how fish cytokines function and whether they have similar roles as in mammals is still a matter of speculation. Here  we have used the zebrafish Danio rerio to gain insights into il4/13a and il4/13b genes and characterized their role under both homeostatic and inflammatory conditions. We established knockouts for both il4/13a and il4/13b genes and showed that they are needed to suppress inflammation in larvae and in the gill mucosa. As a counterpoint  we examined the gills of il10 defective zebrafish and revealed the importance of il10 in maintaining homeostasis in this mucosal tissue. As in mammals  zebrafish il10 appears to have an anti inflammatory function.", "ENA FIRST PUBLIC:2020 05 28|ENA LAST UPDATE:2020 04 16", null, "Protocols: This submission contains RNA seq samples from the gill tissue of five zebrafish strains: Wildtype  il4/13a KO  il4/13b KO  il4/13a;b KO and il10 KO. The strains il4/13a KO  il4/13b KO and il4/13a;b KO were generated using CRISPR/Cas9 system whereas the il10 KO was generated by the Sanger Institute through the Zebrafish Mutation Project Kettleborough RNW  Busch nentwich EM  Harvey SA  Dooley CM  Bruijn E De  Eeden F Van  et al. Europe PMC Funders Group Europe PMC Funders Author Manuscripts A systematic genome wide analysis of zebrafish protein coding gene function. 2013;4967446:494 7. Zebrafish Danio rerio were maintained under standard conditions 28 \u00b0C under a 14 h light/10 h dark cycle within the Biological Services Unit The University of Manchester. Gill tissue was harvested from adult 6 mpf male and female zebrafish. Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen. Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen. Prior to performing RNA seq  the integrity of RNA samples was assessed using a 2200 TapeStation Agilent Technologies according to the manufacturer's instructions. Truseq stranded mRNA Assay. Paired end.", "Sample 6", "SAMEA6786435", "UNIVERSITY OF MANCHESTER", "ENA FIRST PUBLIC:2020 05 28T17:07:47Z|ENA LAST UPDATE:2020 04 16T14:58:14Z|External Id:SAMEA6786435|INSDC center name:UNIVERSITY OF MANCHESTER|INSDC first public:2020 05 28T17:07:47Z|INSDC last update:2020 04 16T14:58:14Z|INSDC status:public|Submitter Id:E MTAB 8958:Sample 6|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:il4/13a  / |organism part:gill|sample name:E MTAB 8958:Sample 6|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Illumina HiSeq 4000 paired end sequencing; Zebrafish il 4 il 10 regulated immunity in gills", "E MTAB 8958:Sample 6 p", "Sample 6 p", "Zebrafish il 4 il 10 regulated immunity in gills", "This submission contains RNA seq samples from the gill tissue of five zebrafish strains: Wildtype  il4/13a KO  il4/13b KO  il4/13a;b KO and il10 KO. The strains il4/13a KO  il4/13b KO and il4/13a;b KO were generated using CRISPR/Cas9 system whereas the  il10 KO was generated by the Sanger Institute through the Zebrafish Mutation Project Kettleborough RNW  Busch nentwich EM  Harvey SA  Dooley CM  Bruijn E De  Eeden F Van  et al. Europe PMC Funders Group Europe PMC Funders Author Manuscripts A systematic genome wide analysis of zebrafish protein coding gene function. 2013;4967446:494\u20137. Zebrafish Danio rerio were maintained under standard conditions 28 \u00b0C under a 14 h light/10 h dark cycle within the Biological Services Unit The University of Manchester. Gill tissue was harvested from adult 6 mpf male and female zebrafish. Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen.  Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen. Prior to performing RNA seq  the integrity of RNA samples was assessed using a 2200 TapeStation Agilent Technologies according to the manufacturer's instructions.  Truseq stranded mRNA Assay. Paired end.", "Experimental Factor: genotype:il4/13a  / ", "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "ERP121190", "Illumina HiSeq 4000 paired end sequencing; Zebrafish il 4 il 10 regulated immunity in gills", "ENA FIRST PUBLIC:2020 05 28|ENA LAST UPDATE:2020 04 16", "FB10_S47_R1_001.fastq.gz FB10_S47_R2_001.fastq.gz", "fastq fastq", 3970723047.0, 26597167.0, "E MTAB 8958:FB10 S47 R", "0:74.63 1:74.66", "A:1037861230;C:915437247;G:922979694;T:1056625196;N:37819680", 74, 74, null, null, 1037861230, 915437247, 922979694, 1056625196, 37819680, "ERX4030561", "ERS4514114", "ERA2508092", "UNIVERSITY OF MANCHESTER|European Nucleotide Archive", "UNIVERSITY OF MANCHESTER|European Nucleotide Archive", 2, 0.92474, 0.92246, 0.0934, 0.0916, 0.69106, 0.69384, 0.49058, 0.48154, 76, 76, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "trueseq", "bulk", "unknown", "unknown", null, "United Kingdom", "2020-04-16", "Adult", "Adult", "Gill", "Respiratory System"], [9857, "ERR4029244", "ERX4030560", "ERS4514113", "ERP121190", "PRJEB37851", "Zebrafish il 4 il 10 regulated immunity in gills", "E-MTAB-8958", "Transcriptome Analysis", "The evolutionary origin of vertebrate type 2 immunity is a topic of great interest. Several studies have focused on the immune cell components of evolutionary older vertebrates such as fish. However  how fish cytokines function and whether they have similar roles as in mammals is still a matter of speculation. Here  we have used the zebrafish Danio rerio to gain insights into il4/13a and il4/13b genes and characterized their role under both homeostatic and inflammatory conditions. We established knockouts for both il4/13a and il4/13b genes and showed that they are needed to suppress inflammation in larvae and in the gill mucosa. As a counterpoint  we examined the gills of il10 defective zebrafish and revealed the importance of il10 in maintaining homeostasis in this mucosal tissue. As in mammals  zebrafish il10 appears to have an anti inflammatory function.", "ENA FIRST PUBLIC:2020 05 28|ENA LAST UPDATE:2020 04 16", null, "Protocols: This submission contains RNA seq samples from the gill tissue of five zebrafish strains: Wildtype  il4/13a KO  il4/13b KO  il4/13a;b KO and il10 KO. The strains il4/13a KO  il4/13b KO and il4/13a;b KO were generated using CRISPR/Cas9 system whereas the il10 KO was generated by the Sanger Institute through the Zebrafish Mutation Project Kettleborough RNW  Busch nentwich EM  Harvey SA  Dooley CM  Bruijn E De  Eeden F Van  et al. Europe PMC Funders Group Europe PMC Funders Author Manuscripts A systematic genome wide analysis of zebrafish protein coding gene function. 2013;4967446:494 7. Zebrafish Danio rerio were maintained under standard conditions 28 \u00b0C under a 14 h light/10 h dark cycle within the Biological Services Unit The University of Manchester. Gill tissue was harvested from adult 6 mpf male and female zebrafish. Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen. Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen. Prior to performing RNA seq  the integrity of RNA samples was assessed using a 2200 TapeStation Agilent Technologies according to the manufacturer's instructions. Truseq stranded mRNA Assay. Paired end.", "Sample 5", "SAMEA6786434", "UNIVERSITY OF MANCHESTER", "ENA FIRST PUBLIC:2020 05 28T17:07:47Z|ENA LAST UPDATE:2020 04 16T14:58:14Z|External Id:SAMEA6786434|INSDC center name:UNIVERSITY OF MANCHESTER|INSDC first public:2020 05 28T17:07:47Z|INSDC last update:2020 04 16T14:58:14Z|INSDC status:public|Submitter Id:E MTAB 8958:Sample 5|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:il4/13a  / |organism part:gill|sample name:E MTAB 8958:Sample 5|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Illumina HiSeq 4000 paired end sequencing; Zebrafish il 4 il 10 regulated immunity in gills", "E MTAB 8958:Sample 5 p", "Sample 5 p", "Zebrafish il 4 il 10 regulated immunity in gills", "This submission contains RNA seq samples from the gill tissue of five zebrafish strains: Wildtype  il4/13a KO  il4/13b KO  il4/13a;b KO and il10 KO. The strains il4/13a KO  il4/13b KO and il4/13a;b KO were generated using CRISPR/Cas9 system whereas the  il10 KO was generated by the Sanger Institute through the Zebrafish Mutation Project Kettleborough RNW  Busch nentwich EM  Harvey SA  Dooley CM  Bruijn E De  Eeden F Van  et al. Europe PMC Funders Group Europe PMC Funders Author Manuscripts A systematic genome wide analysis of zebrafish protein coding gene function. 2013;4967446:494\u20137. Zebrafish Danio rerio were maintained under standard conditions 28 \u00b0C under a 14 h light/10 h dark cycle within the Biological Services Unit The University of Manchester. Gill tissue was harvested from adult 6 mpf male and female zebrafish. Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen.  Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen. Prior to performing RNA seq  the integrity of RNA samples was assessed using a 2200 TapeStation Agilent Technologies according to the manufacturer's instructions.  Truseq stranded mRNA Assay. Paired end.", "Experimental Factor: genotype:il4/13a  / ", "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "ERP121190", "Illumina HiSeq 4000 paired end sequencing; Zebrafish il 4 il 10 regulated immunity in gills", "ENA FIRST PUBLIC:2020 05 28|ENA LAST UPDATE:2020 04 16", "FB9_S46_R1_001.fastq.gz FB9_S46_R2_001.fastq.gz", "fastq fastq", 4267946165.0, 28455064.0, "E MTAB 8958:FB9 S46 R", "0:75.00 1:74.99", "A:1108310330;C:999732020;G:987857724;T:1148193980;N:23852111", 75, 74, null, null, 1108310330, 999732020, 987857724, 1148193980, 23852111, "ERX4030560", "ERS4514113", "ERA2508092", "UNIVERSITY OF MANCHESTER|European Nucleotide Archive", "UNIVERSITY OF MANCHESTER|European Nucleotide Archive", 2, 0.9223, 0.91737, 0.09304, 0.09006, 0.69063, 0.6929, 0.48978, 0.49675, 76, 75, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "trueseq", "bulk", "unknown", "unknown", null, "United Kingdom", "2020-04-16", "Adult", "Adult", "Gill", "Respiratory System"], [9858, "ERR4029243", "ERX4030559", "ERS4514112", "ERP121190", "PRJEB37851", "Zebrafish il 4 il 10 regulated immunity in gills", "E-MTAB-8958", "Transcriptome Analysis", "The evolutionary origin of vertebrate type 2 immunity is a topic of great interest. Several studies have focused on the immune cell components of evolutionary older vertebrates such as fish. However  how fish cytokines function and whether they have similar roles as in mammals is still a matter of speculation. Here  we have used the zebrafish Danio rerio to gain insights into il4/13a and il4/13b genes and characterized their role under both homeostatic and inflammatory conditions. We established knockouts for both il4/13a and il4/13b genes and showed that they are needed to suppress inflammation in larvae and in the gill mucosa. As a counterpoint  we examined the gills of il10 defective zebrafish and revealed the importance of il10 in maintaining homeostasis in this mucosal tissue. As in mammals  zebrafish il10 appears to have an anti inflammatory function.", "ENA FIRST PUBLIC:2020 05 28|ENA LAST UPDATE:2020 04 16", null, "Protocols: This submission contains RNA seq samples from the gill tissue of five zebrafish strains: Wildtype  il4/13a KO  il4/13b KO  il4/13a;b KO and il10 KO. The strains il4/13a KO  il4/13b KO and il4/13a;b KO were generated using CRISPR/Cas9 system whereas the il10 KO was generated by the Sanger Institute through the Zebrafish Mutation Project Kettleborough RNW  Busch nentwich EM  Harvey SA  Dooley CM  Bruijn E De  Eeden F Van  et al. Europe PMC Funders Group Europe PMC Funders Author Manuscripts A systematic genome wide analysis of zebrafish protein coding gene function. 2013;4967446:494 7. Zebrafish Danio rerio were maintained under standard conditions 28 \u00b0C under a 14 h light/10 h dark cycle within the Biological Services Unit The University of Manchester. Gill tissue was harvested from adult 6 mpf male and female zebrafish. Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen. Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen. Prior to performing RNA seq  the integrity of RNA samples was assessed using a 2200 TapeStation Agilent Technologies according to the manufacturer's instructions. Truseq stranded mRNA Assay. Paired end.", "Sample 9", "SAMEA6786433", "UNIVERSITY OF MANCHESTER", "ENA FIRST PUBLIC:2020 05 28T17:07:47Z|ENA LAST UPDATE:2020 04 16T14:58:14Z|External Id:SAMEA6786433|INSDC center name:UNIVERSITY OF MANCHESTER|INSDC first public:2020 05 28T17:07:47Z|INSDC last update:2020 04 16T14:58:14Z|INSDC status:public|Submitter Id:E MTAB 8958:Sample 9|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:il10  / |organism part:gill|sample name:E MTAB 8958:Sample 9|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Illumina HiSeq 4000 paired end sequencing; Zebrafish il 4 il 10 regulated immunity in gills", "E MTAB 8958:Sample 9 p", "Sample 9 p", "Zebrafish il 4 il 10 regulated immunity in gills", "This submission contains RNA seq samples from the gill tissue of five zebrafish strains: Wildtype  il4/13a KO  il4/13b KO  il4/13a;b KO and il10 KO. The strains il4/13a KO  il4/13b KO and il4/13a;b KO were generated using CRISPR/Cas9 system whereas the  il10 KO was generated by the Sanger Institute through the Zebrafish Mutation Project Kettleborough RNW  Busch nentwich EM  Harvey SA  Dooley CM  Bruijn E De  Eeden F Van  et al. Europe PMC Funders Group Europe PMC Funders Author Manuscripts A systematic genome wide analysis of zebrafish protein coding gene function. 2013;4967446:494\u20137. Zebrafish Danio rerio were maintained under standard conditions 28 \u00b0C under a 14 h light/10 h dark cycle within the Biological Services Unit The University of Manchester. Gill tissue was harvested from adult 6 mpf male and female zebrafish. Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen.  Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen. Prior to performing RNA seq  the integrity of RNA samples was assessed using a 2200 TapeStation Agilent Technologies according to the manufacturer's instructions.  Truseq stranded mRNA Assay. Paired end.", "Experimental Factor: genotype:il10  / ", "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "ERP121190", "Illumina HiSeq 4000 paired end sequencing; Zebrafish il 4 il 10 regulated immunity in gills", "ENA FIRST PUBLIC:2020 05 28|ENA LAST UPDATE:2020 04 16", "FB17_S54_R1_001.fastq.gz FB17_S54_R2_001.fastq.gz", "fastq fastq", 3847723303.0, 25764874.0, "E MTAB 8958:FB17 S54 R", "0:74.65 1:74.69", "A:1009950686;C:883047473;G:885821368;T:1035313325;N:33590451", 74, 74, null, null, 1009950686, 883047473, 885821368, 1035313325, 33590451, "ERX4030559", "ERS4514112", "ERA2508092", "UNIVERSITY OF MANCHESTER|European Nucleotide Archive", "UNIVERSITY OF MANCHESTER|European Nucleotide Archive", 2, 0.91088, 0.90785, 0.09899, 0.09623, 0.68822, 0.69055, 0.48686, 0.49337, 76, 75, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "trueseq", "bulk", "unknown", "unknown", null, "United Kingdom", "2020-04-16", "Adult", "Adult", "Gill", "Respiratory System"], [9859, "ERR4029242", "ERX4030558", "ERS4514111", "ERP121190", "PRJEB37851", "Zebrafish il 4 il 10 regulated immunity in gills", "E-MTAB-8958", "Transcriptome Analysis", "The evolutionary origin of vertebrate type 2 immunity is a topic of great interest. Several studies have focused on the immune cell components of evolutionary older vertebrates such as fish. However  how fish cytokines function and whether they have similar roles as in mammals is still a matter of speculation. Here  we have used the zebrafish Danio rerio to gain insights into il4/13a and il4/13b genes and characterized their role under both homeostatic and inflammatory conditions. We established knockouts for both il4/13a and il4/13b genes and showed that they are needed to suppress inflammation in larvae and in the gill mucosa. As a counterpoint  we examined the gills of il10 defective zebrafish and revealed the importance of il10 in maintaining homeostasis in this mucosal tissue. As in mammals  zebrafish il10 appears to have an anti inflammatory function.", "ENA FIRST PUBLIC:2020 05 28|ENA LAST UPDATE:2020 04 16", null, "Protocols: This submission contains RNA seq samples from the gill tissue of five zebrafish strains: Wildtype  il4/13a KO  il4/13b KO  il4/13a;b KO and il10 KO. The strains il4/13a KO  il4/13b KO and il4/13a;b KO were generated using CRISPR/Cas9 system whereas the il10 KO was generated by the Sanger Institute through the Zebrafish Mutation Project Kettleborough RNW  Busch nentwich EM  Harvey SA  Dooley CM  Bruijn E De  Eeden F Van  et al. Europe PMC Funders Group Europe PMC Funders Author Manuscripts A systematic genome wide analysis of zebrafish protein coding gene function. 2013;4967446:494 7. Zebrafish Danio rerio were maintained under standard conditions 28 \u00b0C under a 14 h light/10 h dark cycle within the Biological Services Unit The University of Manchester. Gill tissue was harvested from adult 6 mpf male and female zebrafish. Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen. Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen. Prior to performing RNA seq  the integrity of RNA samples was assessed using a 2200 TapeStation Agilent Technologies according to the manufacturer's instructions. Truseq stranded mRNA Assay. Paired end.", "Sample 12", "SAMEA6786432", "UNIVERSITY OF MANCHESTER", "ENA FIRST PUBLIC:2020 05 28T17:07:47Z|ENA LAST UPDATE:2020 04 16T14:58:14Z|External Id:SAMEA6786432|INSDC center name:UNIVERSITY OF MANCHESTER|INSDC first public:2020 05 28T17:07:47Z|INSDC last update:2020 04 16T14:58:14Z|INSDC status:public|Submitter Id:E MTAB 8958:Sample 12|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:il10  / |organism part:gill|sample name:E MTAB 8958:Sample 12|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Illumina HiSeq 4000 paired end sequencing; Zebrafish il 4 il 10 regulated immunity in gills", "E MTAB 8958:Sample 12 p", "Sample 12 p", "Zebrafish il 4 il 10 regulated immunity in gills", "This submission contains RNA seq samples from the gill tissue of five zebrafish strains: Wildtype  il4/13a KO  il4/13b KO  il4/13a;b KO and il10 KO. The strains il4/13a KO  il4/13b KO and il4/13a;b KO were generated using CRISPR/Cas9 system whereas the  il10 KO was generated by the Sanger Institute through the Zebrafish Mutation Project Kettleborough RNW  Busch nentwich EM  Harvey SA  Dooley CM  Bruijn E De  Eeden F Van  et al. Europe PMC Funders Group Europe PMC Funders Author Manuscripts A systematic genome wide analysis of zebrafish protein coding gene function. 2013;4967446:494\u20137. Zebrafish Danio rerio were maintained under standard conditions 28 \u00b0C under a 14 h light/10 h dark cycle within the Biological Services Unit The University of Manchester. Gill tissue was harvested from adult 6 mpf male and female zebrafish. Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen.  Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen. Prior to performing RNA seq  the integrity of RNA samples was assessed using a 2200 TapeStation Agilent Technologies according to the manufacturer's instructions.  Truseq stranded mRNA Assay. Paired end.", "Experimental Factor: genotype:il10  / ", "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "ERP121190", "Illumina HiSeq 4000 paired end sequencing; Zebrafish il 4 il 10 regulated immunity in gills", "ENA FIRST PUBLIC:2020 05 28|ENA LAST UPDATE:2020 04 16", "FB20_S57_R1_001.fastq.gz FB20_S57_R2_001.fastq.gz", "fastq fastq", 4534822065.0, 30209756.0, "E MTAB 8958:FB20 S57 R", "0:75.06 1:75.06", "A:1181838544;C:1056097162;G:1066594521;T:1207484216;N:22807622", 75, 75, null, null, 1181838544, 1056097162, 1066594521, 1207484216, 22807622, "ERX4030558", "ERS4514111", "ERA2508092", "UNIVERSITY OF MANCHESTER|European Nucleotide Archive", "UNIVERSITY OF MANCHESTER|European Nucleotide Archive", 2, 0.91577, 0.91513, 0.09598, 0.09473, 0.68931, 0.69132, 0.49181, 0.49786, 74, 76, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "trueseq", "bulk", "unknown", "unknown", null, "United Kingdom", "2020-04-16", "Adult", "Adult", "Gill", "Respiratory System"], [9860, "ERR4029241", "ERX4030557", "ERS4514110", "ERP121190", "PRJEB37851", "Zebrafish il 4 il 10 regulated immunity in gills", "E-MTAB-8958", "Transcriptome Analysis", "The evolutionary origin of vertebrate type 2 immunity is a topic of great interest. Several studies have focused on the immune cell components of evolutionary older vertebrates such as fish. However  how fish cytokines function and whether they have similar roles as in mammals is still a matter of speculation. Here  we have used the zebrafish Danio rerio to gain insights into il4/13a and il4/13b genes and characterized their role under both homeostatic and inflammatory conditions. We established knockouts for both il4/13a and il4/13b genes and showed that they are needed to suppress inflammation in larvae and in the gill mucosa. As a counterpoint  we examined the gills of il10 defective zebrafish and revealed the importance of il10 in maintaining homeostasis in this mucosal tissue. As in mammals  zebrafish il10 appears to have an anti inflammatory function.", "ENA FIRST PUBLIC:2020 05 28|ENA LAST UPDATE:2020 04 16", null, "Protocols: This submission contains RNA seq samples from the gill tissue of five zebrafish strains: Wildtype  il4/13a KO  il4/13b KO  il4/13a;b KO and il10 KO. The strains il4/13a KO  il4/13b KO and il4/13a;b KO were generated using CRISPR/Cas9 system whereas the il10 KO was generated by the Sanger Institute through the Zebrafish Mutation Project Kettleborough RNW  Busch nentwich EM  Harvey SA  Dooley CM  Bruijn E De  Eeden F Van  et al. Europe PMC Funders Group Europe PMC Funders Author Manuscripts A systematic genome wide analysis of zebrafish protein coding gene function. 2013;4967446:494 7. Zebrafish Danio rerio were maintained under standard conditions 28 \u00b0C under a 14 h light/10 h dark cycle within the Biological Services Unit The University of Manchester. Gill tissue was harvested from adult 6 mpf male and female zebrafish. Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen. Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen. Prior to performing RNA seq  the integrity of RNA samples was assessed using a 2200 TapeStation Agilent Technologies according to the manufacturer's instructions. Truseq stranded mRNA Assay. Paired end.", "Sample 11", "SAMEA6786431", "UNIVERSITY OF MANCHESTER", "ENA FIRST PUBLIC:2020 05 28T17:07:47Z|ENA LAST UPDATE:2020 04 16T14:58:14Z|External Id:SAMEA6786431|INSDC center name:UNIVERSITY OF MANCHESTER|INSDC first public:2020 05 28T17:07:47Z|INSDC last update:2020 04 16T14:58:14Z|INSDC status:public|Submitter Id:E MTAB 8958:Sample 11|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:il10  / |organism part:gill|sample name:E MTAB 8958:Sample 11|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Illumina HiSeq 4000 paired end sequencing; Zebrafish il 4 il 10 regulated immunity in gills", "E MTAB 8958:Sample 11 p", "Sample 11 p", "Zebrafish il 4 il 10 regulated immunity in gills", "This submission contains RNA seq samples from the gill tissue of five zebrafish strains: Wildtype  il4/13a KO  il4/13b KO  il4/13a;b KO and il10 KO. The strains il4/13a KO  il4/13b KO and il4/13a;b KO were generated using CRISPR/Cas9 system whereas the  il10 KO was generated by the Sanger Institute through the Zebrafish Mutation Project Kettleborough RNW  Busch nentwich EM  Harvey SA  Dooley CM  Bruijn E De  Eeden F Van  et al. Europe PMC Funders Group Europe PMC Funders Author Manuscripts A systematic genome wide analysis of zebrafish protein coding gene function. 2013;4967446:494\u20137. Zebrafish Danio rerio were maintained under standard conditions 28 \u00b0C under a 14 h light/10 h dark cycle within the Biological Services Unit The University of Manchester. Gill tissue was harvested from adult 6 mpf male and female zebrafish. Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen.  Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen. Prior to performing RNA seq  the integrity of RNA samples was assessed using a 2200 TapeStation Agilent Technologies according to the manufacturer's instructions.  Truseq stranded mRNA Assay. Paired end.", "Experimental Factor: genotype:il10  / ", "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "ERP121190", "Illumina HiSeq 4000 paired end sequencing; Zebrafish il 4 il 10 regulated immunity in gills", "ENA FIRST PUBLIC:2020 05 28|ENA LAST UPDATE:2020 04 16", "FB19_S56_R1_001.fastq.gz FB19_S56_R2_001.fastq.gz", "fastq fastq", 5070571422.0, 33987501.0, "E MTAB 8958:FB19 S56 R", "0:74.58 1:74.61", "A:1327496786;C:1163563377;G:1162870630;T:1365191685;N:51448944", 74, 74, null, null, 1327496786, 1163563377, 1162870630, 1365191685, 51448944, "ERX4030557", "ERS4514110", "ERA2508092", "UNIVERSITY OF MANCHESTER|European Nucleotide Archive", "UNIVERSITY OF MANCHESTER|European Nucleotide Archive", 2, 0.91091, 0.91003, 0.09944, 0.09748, 0.68745, 0.69016, 0.49706, 0.49287, 76, 75, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "trueseq", "bulk", "unknown", "unknown", null, "United Kingdom", "2020-04-16", "Adult", "Adult", "Gill", "Respiratory System"], [9861, "ERR4029240", "ERX4030556", "ERS4514109", "ERP121190", "PRJEB37851", "Zebrafish il 4 il 10 regulated immunity in gills", "E-MTAB-8958", "Transcriptome Analysis", "The evolutionary origin of vertebrate type 2 immunity is a topic of great interest. Several studies have focused on the immune cell components of evolutionary older vertebrates such as fish. However  how fish cytokines function and whether they have similar roles as in mammals is still a matter of speculation. Here  we have used the zebrafish Danio rerio to gain insights into il4/13a and il4/13b genes and characterized their role under both homeostatic and inflammatory conditions. We established knockouts for both il4/13a and il4/13b genes and showed that they are needed to suppress inflammation in larvae and in the gill mucosa. As a counterpoint  we examined the gills of il10 defective zebrafish and revealed the importance of il10 in maintaining homeostasis in this mucosal tissue. As in mammals  zebrafish il10 appears to have an anti inflammatory function.", "ENA FIRST PUBLIC:2020 05 28|ENA LAST UPDATE:2020 04 16", null, "Protocols: This submission contains RNA seq samples from the gill tissue of five zebrafish strains: Wildtype  il4/13a KO  il4/13b KO  il4/13a;b KO and il10 KO. The strains il4/13a KO  il4/13b KO and il4/13a;b KO were generated using CRISPR/Cas9 system whereas the il10 KO was generated by the Sanger Institute through the Zebrafish Mutation Project Kettleborough RNW  Busch nentwich EM  Harvey SA  Dooley CM  Bruijn E De  Eeden F Van  et al. Europe PMC Funders Group Europe PMC Funders Author Manuscripts A systematic genome wide analysis of zebrafish protein coding gene function. 2013;4967446:494 7. Zebrafish Danio rerio were maintained under standard conditions 28 \u00b0C under a 14 h light/10 h dark cycle within the Biological Services Unit The University of Manchester. Gill tissue was harvested from adult 6 mpf male and female zebrafish. Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen. Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen. Prior to performing RNA seq  the integrity of RNA samples was assessed using a 2200 TapeStation Agilent Technologies according to the manufacturer's instructions. Truseq stranded mRNA Assay. Paired end.", "Sample 10", "SAMEA6786430", "UNIVERSITY OF MANCHESTER", "ENA FIRST PUBLIC:2020 05 28T17:07:47Z|ENA LAST UPDATE:2020 04 16T14:58:14Z|External Id:SAMEA6786430|INSDC center name:UNIVERSITY OF MANCHESTER|INSDC first public:2020 05 28T17:07:47Z|INSDC last update:2020 04 16T14:58:14Z|INSDC status:public|Submitter Id:E MTAB 8958:Sample 10|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|genotype:il10  / |organism part:gill|sample name:E MTAB 8958:Sample 10|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Illumina HiSeq 4000 paired end sequencing; Zebrafish il 4 il 10 regulated immunity in gills", "E MTAB 8958:Sample 10 p", "Sample 10 p", "Zebrafish il 4 il 10 regulated immunity in gills", "This submission contains RNA seq samples from the gill tissue of five zebrafish strains: Wildtype  il4/13a KO  il4/13b KO  il4/13a;b KO and il10 KO. The strains il4/13a KO  il4/13b KO and il4/13a;b KO were generated using CRISPR/Cas9 system whereas the  il10 KO was generated by the Sanger Institute through the Zebrafish Mutation Project Kettleborough RNW  Busch nentwich EM  Harvey SA  Dooley CM  Bruijn E De  Eeden F Van  et al. Europe PMC Funders Group Europe PMC Funders Author Manuscripts A systematic genome wide analysis of zebrafish protein coding gene function. 2013;4967446:494\u20137. Zebrafish Danio rerio were maintained under standard conditions 28 \u00b0C under a 14 h light/10 h dark cycle within the Biological Services Unit The University of Manchester. Gill tissue was harvested from adult 6 mpf male and female zebrafish. Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen.  Gills were harvested from four fish for each strain n=4  one fish per sample and total RNA was isolated from each sample using the RNeasy mini kit Qiagen. Prior to performing RNA seq  the integrity of RNA samples was assessed using a 2200 TapeStation Agilent Technologies according to the manufacturer's instructions.  Truseq stranded mRNA Assay. Paired end.", "Experimental Factor: genotype:il10  / ", "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "ERP121190", "Illumina HiSeq 4000 paired end sequencing; Zebrafish il 4 il 10 regulated immunity in gills", "ENA FIRST PUBLIC:2020 05 28|ENA LAST UPDATE:2020 04 16", "FB18_S55_R1_001.fastq.gz FB18_S55_R2_001.fastq.gz", "fastq fastq", 4411576023.0, 29389076.0, "E MTAB 8958:FB18 S55 R", "0:75.05 1:75.06", "A:1153821133;C:1024593137;G:1029312154;T:1181989770;N:21859829", 75, 75, null, null, 1153821133, 1024593137, 1029312154, 1181989770, 21859829, "ERX4030556", "ERS4514109", "ERA2508092", "UNIVERSITY OF MANCHESTER|European Nucleotide Archive", "UNIVERSITY OF MANCHESTER|European Nucleotide Archive", 2, 0.90933, 0.90999, 0.09581, 0.09404, 0.68917, 0.69199, 0.49527, 0.49791, 76, 76, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "random_priming", "trueseq", "bulk", "unknown", "unknown", null, "United Kingdom", "2020-04-16", "Adult", "Adult", "Gill", "Respiratory System"], [56007, "SRR10895915", "SRX7564564", "SRS6001797", "SRP241982", "PRJNA599026", "Project of basal ray finned fishes", "PRJNA599026", "Other", "The project is to study the oldest fish lineages in the ray finned fishes. Although they belong to fish  their body structure and behavior remain highly similar to that of the tetrapods. Through comparative genome analysis with living vertebrates  we provides insights into the molecular basis of terrestrial adaptation of basal ray finned fishes.", null, "pubmed:33545088;pubmed:12470943", null, null, "Zebrafish 002", null, "strain:not collected|isolate:not collected|breed:not collected|cultivar:not collected|ecotype:not collected|age:not collected|dev stage:Adult|sex:not determined|tissue:Gill|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio : adult", "CL100101924 L01 4", "CL100101924 L01 4", null, null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM", "PAIRED", "BGISEQ", "BGISEQ-500", null, "SRP241982", null, null, "CL100101924_L01_542_2.fq.gz CL100101924_L01_542_1.fq.gz", "fastq fastq", 9311999200.0, 93119992.0, "CL100101924 L01 542 1.fq.gz", "0:100 1:100", "A:2545207764;C:2065502824;G:2111148485;T:2578747353;N:11392774", 100, 100, null, null, 2545207764, 2065502824, 2111148485, 2578747353, 11392774, "SRX7564564", "SRS6001797", "SRA1026516", "BGI|BGI-Research", "BGI", 1, 0.91339, null, 0.10797, null, 0.69792, null, 0.52261, null, 100, null, "B", null, "usable mapping rate", "bgi", "bgi", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2020-01-15", "Adult", "Adult", "Gill", "Respiratory System"], [56008, "SRR10895916", "SRX7564563", "SRS6001797", "SRP241982", "PRJNA599026", "Project of basal ray finned fishes", "PRJNA599026", "Other", "The project is to study the oldest fish lineages in the ray finned fishes. Although they belong to fish  their body structure and behavior remain highly similar to that of the tetrapods. Through comparative genome analysis with living vertebrates  we provides insights into the molecular basis of terrestrial adaptation of basal ray finned fishes.", null, "pubmed:33545088;pubmed:12470943", null, null, "Zebrafish 002", null, "strain:not collected|isolate:not collected|breed:not collected|cultivar:not collected|ecotype:not collected|age:not collected|dev stage:Adult|sex:not determined|tissue:Gill|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio : adult", "CL100101924 L01 3", "CL100101924 L01 3", null, null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM", "PAIRED", "BGISEQ", "BGISEQ-500", null, "SRP241982", null, null, "CL100101924_L01_541_1.fq.gz CL100101924_L01_541_2.fq.gz", "fastq fastq", 10478802600.0, 104788026.0, "CL100101924 L01 541 1.fq.gz", "0:100 1:100", "A:2866250643;C:2322460084;G:2375280057;T:2901876128;N:12935688", 100, 100, null, null, 2866250643, 2322460084, 2375280057, 2901876128, 12935688, "SRX7564563", "SRS6001797", "SRA1026516", "BGI|BGI-Research", "BGI", 1, 0.9209, null, 0.11359, null, 0.69858, null, 0.53578, null, 100, null, "B", null, "usable mapping rate", "bgi", "bgi", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2020-01-15", "Adult", "Adult", "Gill", "Respiratory System"], [56009, "SRR10895917", "SRX7564562", "SRS6001797", "SRP241982", "PRJNA599026", "Project of basal ray finned fishes", "PRJNA599026", "Other", "The project is to study the oldest fish lineages in the ray finned fishes. Although they belong to fish  their body structure and behavior remain highly similar to that of the tetrapods. Through comparative genome analysis with living vertebrates  we provides insights into the molecular basis of terrestrial adaptation of basal ray finned fishes.", null, "pubmed:33545088;pubmed:12470943", null, null, "Zebrafish 002", null, "strain:not collected|isolate:not collected|breed:not collected|cultivar:not collected|ecotype:not collected|age:not collected|dev stage:Adult|sex:not determined|tissue:Gill|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio : adult", "CL100101924 L01 2", "CL100101924 L01 2", null, null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM", "PAIRED", "BGISEQ", "BGISEQ-500", null, "SRP241982", null, null, "CL100101924_L01_543_1.fq.gz CL100101924_L01_543_2.fq.gz", "fastq fastq", 10370355000.0, 103703550.0, "CL100101924 L01 543 1.fq.gz", "0:100 1:100", "A:2824450277;C:2308377166;G:2360285452;T:2864390654;N:12851451", 100, 100, null, null, 2824450277, 2308377166, 2360285452, 2864390654, 12851451, "SRX7564562", "SRS6001797", "SRA1026516", "BGI|BGI-Research", "BGI", 1, 0.91018, null, 0.09651, null, 0.70224, null, 0.53831, null, 100, null, "B", null, "usable mapping rate", "bgi", "bgi", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2020-01-15", "Adult", "Adult", "Gill", "Respiratory System"], [56010, "SRR10895918", "SRX7564561", "SRS6001797", "SRP241982", "PRJNA599026", "Project of basal ray finned fishes", "PRJNA599026", "Other", "The project is to study the oldest fish lineages in the ray finned fishes. Although they belong to fish  their body structure and behavior remain highly similar to that of the tetrapods. Through comparative genome analysis with living vertebrates  we provides insights into the molecular basis of terrestrial adaptation of basal ray finned fishes.", null, "pubmed:33545088;pubmed:12470943", null, null, "Zebrafish 002", null, "strain:not collected|isolate:not collected|breed:not collected|cultivar:not collected|ecotype:not collected|age:not collected|dev stage:Adult|sex:not determined|tissue:Gill|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio : adult", "CL100101924 L01", "CL100101924 L01", null, null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM", "PAIRED", "BGISEQ", "BGISEQ-500", null, "SRP241982", null, null, "CL100101924_L01_544_2.fq.gz CL100101924_L01_544_1.fq.gz", "fastq fastq", 8686710000.0, 86867100.0, "CL100101924 L01 544 1.fq.gz", "0:100 1:100", "A:2361387592;C:1935295339;G:1982562294;T:2396806096;N:10658679", 100, 100, null, null, 2361387592, 1935295339, 1982562294, 2396806096, 10658679, "SRX7564561", "SRS6001797", "SRA1026516", "BGI|BGI-Research", "BGI", 1, 0.90603, null, 0.12145, null, 0.69412, null, 0.52836, null, 100, null, "B", null, "usable mapping rate", "bgi", "bgi", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2020-01-15", "Adult", "Adult", "Gill", "Respiratory System"], [56011, "SRR10895919", "SRX7564560", "SRS6001797", "SRP241982", "PRJNA599026", "Project of basal ray finned fishes", "PRJNA599026", "Other", "The project is to study the oldest fish lineages in the ray finned fishes. Although they belong to fish  their body structure and behavior remain highly similar to that of the tetrapods. Through comparative genome analysis with living vertebrates  we provides insights into the molecular basis of terrestrial adaptation of basal ray finned fishes.", null, "pubmed:33545088;pubmed:12470943", null, null, "Zebrafish 002", null, "strain:not collected|isolate:not collected|breed:not collected|cultivar:not collected|ecotype:not collected|age:not collected|dev stage:Adult|sex:not determined|tissue:Gill|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio : adult", "CL100103858 L02", "CL100103858 L02", null, null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM", "PAIRED", "BGISEQ", "BGISEQ-500", null, "SRP241982", null, null, "CL100103858_L02_530_1.fq.gz CL100103858_L02_530_2.fq.gz", "fastq fastq", 9390776200.0, 93907762.0, "CL100103858 L02 530 1.fq.gz", "0:100 1:100", "A:2561256067;C:2084956400;G:2134804271;T:2596713447;N:13046015", 100, 100, null, null, 2561256067, 2084956400, 2134804271, 2596713447, 13046015, "SRX7564560", "SRS6001797", "SRA1026516", "BGI|BGI-Research", "BGI", 1, 0.91926, null, 0.11028, null, 0.69485, null, 0.54756, null, 100, null, "B", null, "usable mapping rate", "bgi", "bgi", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2020-01-15", "Adult", "Adult", "Gill", "Respiratory System"], [63052, "SRR13587425", "SRX9982120", "SRS8153603", "SRP303929", "PRJNA698005", "Danio rerio Raw sequence reads", "PRJNA698005", "Whole Genome Sequencing", "Danio rerio Raw RNA sequence reads", null, null, null, "Model organism or animal sample from Danio rerio", "The gill of Danio rerio", null, "isolate:river|age:adult|sex:not determined|tissue:gill|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "9ZG", "9ZG", "9ZG", "The gill experiment repeat 3 of Danio rerio", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM", "PAIRED", "BGISEQ", "BGISEQ-500", null, "SRP303929", null, null, "9ZG_1.fq.gz 9ZG_2.fq.gz", "fastq fastq", 8056935600.0, 53712904.0, "9ZG 1.fq.gz", "0:150 1:150", "A:2282673113;C:1739857627;G:1727599599;T:2306805261;N:0", 150, 150, null, null, 2282673113, 1739857627, 1727599599, 2306805261, 0, "SRX9982120", "SRS8153603", "SRA1189632", "Center for Ecological and Environmental Sciences|Northwestern Polytechnical University", "Center for Ecological and Environmental Sciences", 1, 0.89727, null, 0.12013, null, 0.70043, null, 0.53736, null, 150, null, "B", null, "usable mapping rate", "bgi", "bgi", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2021-01-30", "Adult", "Adult", "Gill", "Respiratory System"], [63053, "SRR13587426", "SRX9982119", "SRS8153603", "SRP303929", "PRJNA698005", "Danio rerio Raw sequence reads", "PRJNA698005", "Whole Genome Sequencing", "Danio rerio Raw RNA sequence reads", null, null, null, "Model organism or animal sample from Danio rerio", "The gill of Danio rerio", null, "isolate:river|age:adult|sex:not determined|tissue:gill|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "24ZG", "24ZG", "24ZG", "The gill control repeat 3 of Danio rerio", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM", "PAIRED", "BGISEQ", "BGISEQ-500", null, "SRP303929", null, null, "24ZG_1.fq.gz 24ZG_2.fq.gz", "fastq fastq", 8223747600.0, 54824984.0, "24ZG 1.fq.gz", "0:150 1:150", "A:2300974904;C:1808864978;G:1782331401;T:2331317250;N:259067", 150, 150, null, null, 2300974904, 1808864978, 1782331401, 2331317250, 259067, "SRX9982119", "SRS8153603", "SRA1189632", "Center for Ecological and Environmental Sciences|Northwestern Polytechnical University", "Center for Ecological and Environmental Sciences", 1, 0.89723, null, 0.1296, null, 0.69392, null, 0.52375, null, 150, null, "B", null, "usable mapping rate", "bgi", "bgi", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2021-01-30", "Adult", "Adult", "Gill", "Respiratory System"], [63054, "SRR13587427", "SRX9982118", "SRS8153603", "SRP303929", "PRJNA698005", "Danio rerio Raw sequence reads", "PRJNA698005", "Whole Genome Sequencing", "Danio rerio Raw RNA sequence reads", null, null, null, "Model organism or animal sample from Danio rerio", "The gill of Danio rerio", null, "isolate:river|age:adult|sex:not determined|tissue:gill|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "8ZG", "8ZG", "8ZG", "The gill experiment repeat 2 of Danio rerio", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM", "PAIRED", "BGISEQ", "BGISEQ-500", null, "SRP303929", null, null, "8ZG_1.fq.gz 8ZG_2.fq.gz", "fastq fastq", 8214407100.0, 54762714.0, "8ZG 1.fq.gz", "0:150 1:150", "A:2359573564;C:1747625839;G:1728726696;T:2377197140;N:1283861", 150, 150, null, null, 2359573564, 1747625839, 1728726696, 2377197140, 1283861, "SRX9982118", "SRS8153603", "SRA1189632", "Center for Ecological and Environmental Sciences|Northwestern Polytechnical University", "Center 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