{"database": "metadata", "table": "run_metadata", "is_view": false, "human_description_en": "where experiment.library_selection = \"PolyA\", technology = \"unknown\" and tissue_curation = \"Heart\"", "rows": [[31715, "SRR28508805", "SRX24109965", "SRS20897113", "SRP498846", "PRJNA1094457", "Danio rerio Transcriptome or Gene expression", "PRJNA1094457", "Other", "Atrial cardiomyopathy  which often comes with underlying genetic defects  has been recognized as a possible substrate of atrial fibrillation. MYH6 encodes a myosin heavy chain a MHC  predominantly expressed in the atria and pivotal in sarcomere organization and muscle contraction. However  the role of MYH6 in atrial diseases has not been fully elucidated and the genetic cause of atrial cardiomyopathy needs further investigation. Here  we employed CRISPR/Cas9 to generate myh6 knockout zebrafish  assessing cardiac function through diverse methodologies  including video analysis  ECG  echocardiography  transmission electron microscopy  and transcriptomic sequencing. Our findings revealed that myh6 defects in zebrafish led to impaired atrial and ventricular function and disordered sarcomere. This research underscores the pivotal role of MYH6 in maintaining atrial function and development. The observed connections between sarcomeric gene variants and atrial cardiomyopathy contribute to our understanding of the genetic basis of atrial fibrillation.", null, null, null, "myh6+/  3", "Sample 6", null, "strain:not collected|isolate:not collected|breed:not collected|cultivar:not collected|ecotype:not collected|age:6 mpf|dev stage:not collected|collection date:2023 09 03|geo loc name:not collected|sex:pooled male and female|tissue:heart|genotype:myh6+/ |BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "myh6+/  3", "Sample 6", "Sample 6", "Sequencing data were analyzed by R studio Version 2023.03.0+386. 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MYH6 encodes a myosin heavy chain a MHC  predominantly expressed in the atria and pivotal in sarcomere organization and muscle contraction. However  the role of MYH6 in atrial diseases has not been fully elucidated and the genetic cause of atrial cardiomyopathy needs further investigation. Here  we employed CRISPR/Cas9 to generate myh6 knockout zebrafish  assessing cardiac function through diverse methodologies  including video analysis  ECG  echocardiography  transmission electron microscopy  and transcriptomic sequencing. Our findings revealed that myh6 defects in zebrafish led to impaired atrial and ventricular function and disordered sarcomere. This research underscores the pivotal role of MYH6 in maintaining atrial function and development. The observed connections between sarcomeric gene variants and atrial cardiomyopathy contribute to our understanding of the genetic basis of atrial fibrillation.", null, null, null, "myh6+/  2", "Sample 5", null, "strain:not collected|isolate:not collected|breed:not collected|cultivar:not collected|ecotype:not collected|age:6 mpf|dev stage:not collected|collection date:2023 09 03|geo loc name:not collected|sex:pooled male and female|tissue:heart|genotype:myh6+/ |BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "myh6+/  2", "Sample 5", "Sample 5", "Sequencing data were analyzed by R studio Version 2023.03.0+386. 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MYH6 encodes a myosin heavy chain a MHC  predominantly expressed in the atria and pivotal in sarcomere organization and muscle contraction. However  the role of MYH6 in atrial diseases has not been fully elucidated and the genetic cause of atrial cardiomyopathy needs further investigation. Here  we employed CRISPR/Cas9 to generate myh6 knockout zebrafish  assessing cardiac function through diverse methodologies  including video analysis  ECG  echocardiography  transmission electron microscopy  and transcriptomic sequencing. Our findings revealed that myh6 defects in zebrafish led to impaired atrial and ventricular function and disordered sarcomere. This research underscores the pivotal role of MYH6 in maintaining atrial function and development. The observed connections between sarcomeric gene variants and atrial cardiomyopathy contribute to our understanding of the genetic basis of atrial fibrillation.", null, null, null, "myh6+/  1", "Sample 4", null, "strain:not collected|isolate:not collected|breed:not collected|cultivar:not collected|ecotype:not collected|age:6 mpf|dev stage:not collected|collection date:2023 09 03|geo loc name:not collected|sex:pooled male and female|tissue:heart|genotype:myh6+/ |BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "myh6+/  1", "Sample 4", "Sample 4", "Sequencing data were analyzed by R studio Version 2023.03.0+386. 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The observed connections between sarcomeric gene variants and atrial cardiomyopathy contribute to our understanding of the genetic basis of atrial fibrillation.", null, null, null, "WT 3", "Sample 3", null, "strain:not collected|isolate:not collected|breed:not collected|cultivar:not collected|ecotype:not collected|age:6 mpf|dev stage:not collected|collection date:2023 09 03|geo loc name:not collected|sex:pooled male and female|tissue:heart|genotype:wild type|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "WT 3", "Sample 3", "Sample 3", "Sequencing data were analyzed by R studio Version 2023.03.0+386. 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MYH6 encodes a myosin heavy chain a MHC  predominantly expressed in the atria and pivotal in sarcomere organization and muscle contraction. However  the role of MYH6 in atrial diseases has not been fully elucidated and the genetic cause of atrial cardiomyopathy needs further investigation. Here  we employed CRISPR/Cas9 to generate myh6 knockout zebrafish  assessing cardiac function through diverse methodologies  including video analysis  ECG  echocardiography  transmission electron microscopy  and transcriptomic sequencing. Our findings revealed that myh6 defects in zebrafish led to impaired atrial and ventricular function and disordered sarcomere. This research underscores the pivotal role of MYH6 in maintaining atrial function and development. The observed connections between sarcomeric gene variants and atrial cardiomyopathy contribute to our understanding of the genetic basis of atrial fibrillation.", null, null, null, "WT 2", "Sample 2", null, "strain:not collected|isolate:not collected|breed:not collected|cultivar:not collected|ecotype:not collected|age:6 mpf|dev stage:not collected|collection date:2023 09 03|geo loc name:not collected|sex:pooled male and female|tissue:heart|genotype:wild type|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "WT 2", "Sample 2", "Sample 2", "Sequencing data were analyzed by R studio Version 2023.03.0+386. 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The observed connections between sarcomeric gene variants and atrial cardiomyopathy contribute to our understanding of the genetic basis of atrial fibrillation.", null, null, null, "WT 1", "Sample 1", null, "strain:not collected|isolate:not collected|breed:not collected|cultivar:not collected|ecotype:not collected|age:6 mpf|dev stage:not collected|collection date:2023 09 03|geo loc name:not collected|sex:pooled male and female|tissue:heart|genotype:wild type|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "WT 1", "Sample 1", "Sample 1", "Sequencing data were analyzed by R studio Version 2023.03.0+386. 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This project contains raw sequencing data sets that were used to identify that klf1  a Kruppel like transcription factor essential for red blood cell development  is also necessary and sufficient in the myocardium for the induction of cardiomyocyte dedifferentiation and proliferation in adult zebrafish hearts", null, null, "WT", null, "WT", null, "strain:EKW|breed:n1|cultivar:n1|ecotype:n1|dev stage:Adult|sex:pooled male and female|tissue:hearts|genotype:WT|sample type:tissue sample|treatment:untreated|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of zebrafish cardiac tissue", "WT1 RNASEQ", "WT1 RNASEQ", "Tissue was resuspended in 700ul Trizol and total RNA was extracted using miRNeasy kit following manufacturers description. Total RNA integrity was assessed and quantified on a RNA nano Agilent chip following manufacturers description. 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