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Read counts for each gene were calculated using featureCounts.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP496008", null, null, "M22-3_1.fq.gz M22-3_2.fq.gz", "fastq fastq", 6312346800.0, 21041156.0, "M22 3 1.fq.gz", "0:150 1:150", "A:1638152183;C:1500304435;G:1533591340;T:1640250617;N:48225", 150, 150, null, null, 1638152183, 1500304435, 1533591340, 1640250617, 48225, "SRX23975262", "SRS20773680", "SRA1825755", "shanghai ocean university|College of Fisheries and Life Science", "shanghai ocean university", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2024-03-18", "Adult", "Adult", "Gonad", "Reproductive System"], [26551, "SRR28370192", "SRX23975261", "SRS20773679", "SRP496008", "PRJNA1020641", "Danio rerio strain:AB strain Raw sequence reads", "PRJNA1020641", "Whole Genome Sequencing", "post different low temperature treatments for 14 days  transcriptome sequencing was conducted on ovaries to analyze the metabolic state under low temperatures.", null, null, null, null, "M22 4 1", null, "strain:not applicable|age:7|dev stage:month|collection date:2021 08 23|geo loc name:not applicable|sex:female|tissue:ovary|replicate:replicate=biological replicate 23|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA seq of ovary: adult female zebrafish", "E23", "E23", "Strand specific RNA seq libraries were prepared using the VAHTS Stranded mRNA seq Library Prep Kit for Illumina v2 Vazyme Biotech  China and subjected to 150bp paired end sequencing on the Illumina Novaseq 6000 platform. Trimmomatic software was employed to remove low quality reads and sequencing adapters  followed by the alignment of clean reads to the zebrafish reference genome GRCz11 v106 using STAR software. Read counts for each gene were calculated using featureCounts.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP496008", null, null, "M22-4_1.fq.gz M22-4_2.fq.gz", "fastq fastq", 7163095500.0, 23876985.0, "M22 4 1.fq.gz", "0:150 1:150", "A:1851012240;C:1707016293;G:1748790263;T:1856222899;N:53805", 150, 150, null, null, 1851012240, 1707016293, 1748790263, 1856222899, 53805, "SRX23975261", "SRS20773679", "SRA1825755", "shanghai ocean university|College of Fisheries and Life Science", "shanghai ocean university", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2024-03-18", "Adult", "Adult", "Gonad", "Reproductive System"], [26552, "SRR28370193", "SRX23975260", "SRS20773678", "SRP496008", "PRJNA1020641", "Danio rerio strain:AB strain Raw sequence reads", "PRJNA1020641", "Whole Genome Sequencing", "post different low temperature treatments for 14 days  transcriptome sequencing was conducted on ovaries to analyze the metabolic state under low temperatures.", null, null, null, null, "L13 1 1", null, "strain:not applicable|age:7|dev stage:month|collection date:2021 08 23|geo loc name:not applicable|sex:female|tissue:ovary|replicate:replicate=biological replicate 25|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA seq of ovary: adult female zebrafish", "E25", "E25", "Strand specific RNA seq libraries were prepared using the VAHTS Stranded mRNA seq Library Prep Kit for Illumina v2 Vazyme Biotech  China and subjected to 150bp paired end sequencing on the Illumina Novaseq 6000 platform. Trimmomatic software was employed to remove low quality reads and sequencing adapters  followed by the alignment of clean reads to the zebrafish reference genome GRCz11 v106 using STAR software. Read counts for each gene were calculated using featureCounts.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP496008", null, null, "L13-1_1.fq.gz L13-1_2.fq.gz", "fastq fastq", 6191424300.0, 20638081.0, "L13 1 1.fq.gz", "0:150 1:150", "A:1605753886;C:1471890545;G:1506901769;T:1606830995;N:47105", 150, 150, null, null, 1605753886, 1471890545, 1506901769, 1606830995, 47105, "SRX23975260", "SRS20773678", "SRA1825755", "shanghai ocean university|College of Fisheries and Life Science", "shanghai ocean university", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2024-03-18", "Adult", "Adult", "Gonad", "Reproductive System"], [26553, "SRR28370194", "SRX23975259", "SRS20773677", "SRP496008", "PRJNA1020641", "Danio rerio strain:AB strain Raw sequence reads", "PRJNA1020641", "Whole Genome Sequencing", "post different low temperature treatments for 14 days  transcriptome sequencing was conducted on ovaries to analyze the metabolic state under low temperatures.", null, null, null, null, "M22 1 1", null, "strain:not applicable|age:7|dev stage:month|collection date:2021 08 23|geo loc name:not applicable|sex:female|tissue:ovary|replicate:replicate=biological replicate 17|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA seq of ovary: adult female zebrafish", "E17", "E17", "Strand specific RNA seq libraries were prepared using the VAHTS Stranded mRNA seq Library Prep Kit for Illumina v2 Vazyme Biotech  China and subjected to 150bp paired end sequencing on the Illumina Novaseq 6000 platform. Trimmomatic software was employed to remove low quality reads and sequencing adapters  followed by the alignment of clean reads to the zebrafish reference genome GRCz11 v106 using STAR software. Read counts for each gene were calculated using featureCounts.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP496008", null, null, "M22-1_1.fq.gz M22-1_2.fq.gz", "fastq fastq", 6102383400.0, 20341278.0, "M22 1 1.fq.gz", "0:150 1:150", "A:1577012976;C:1455984420;G:1487747979;T:1581590987;N:47038", 150, 150, null, null, 1577012976, 1455984420, 1487747979, 1581590987, 47038, "SRX23975259", "SRS20773677", "SRA1825755", "shanghai ocean university|College of Fisheries and Life Science", "shanghai ocean university", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2024-03-18", "Adult", "Adult", "Gonad", "Reproductive System"], [26554, "SRR28370195", "SRX23975258", "SRS20773676", "SRP496008", "PRJNA1020641", "Danio rerio strain:AB strain Raw sequence reads", "PRJNA1020641", "Whole Genome Sequencing", "post different low temperature treatments for 14 days  transcriptome sequencing was conducted on ovaries to analyze the metabolic state under low temperatures.", null, null, null, null, "M20 4 1", null, "strain:not applicable|age:7|dev stage:month|collection date:2021 08 23|geo loc name:not applicable|sex:female|tissue:ovary|replicate:replicate=biological replicate 15|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA seq of ovary: adult female zebrafish", "E15", "E15", "Strand specific RNA seq libraries were prepared using the VAHTS Stranded mRNA seq Library Prep Kit for Illumina v2 Vazyme Biotech  China and subjected to 150bp paired end sequencing on the Illumina Novaseq 6000 platform. Trimmomatic software was employed to remove low quality reads and sequencing adapters  followed by the alignment of clean reads to the zebrafish reference genome GRCz11 v106 using STAR software. Read counts for each gene were calculated using featureCounts.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP496008", null, null, "M20-4_1.fq.gz M20-4_2.fq.gz", "fastq fastq", 6636566100.0, 22121887.0, "M20 4 1.fq.gz", "0:150 1:150", "A:1715667953;C:1580553403;G:1620418612;T:1719875870;N:50262", 150, 150, null, null, 1715667953, 1580553403, 1620418612, 1719875870, 50262, "SRX23975258", "SRS20773676", "SRA1825755", "shanghai ocean university|College of Fisheries and Life Science", "shanghai ocean university", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2024-03-18", "Adult", "Adult", "Gonad", "Reproductive System"], [26555, "SRR28370196", "SRX23975257", "SRS20773675", "SRP496008", "PRJNA1020641", "Danio rerio strain:AB strain Raw sequence reads", "PRJNA1020641", "Whole Genome Sequencing", "post different low temperature treatments for 14 days  transcriptome sequencing was conducted on ovaries to analyze the metabolic state under low temperatures.", null, null, null, null, "M20 3 1", null, "strain:not applicable|age:7|dev stage:month|collection date:2021 08 23|geo loc name:not applicable|sex:female|tissue:ovary|replicate:replicate=biological replicate 13|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA seq of ovary: adult female zebrafish", "E13", "E13", "Strand specific RNA seq libraries were prepared using the VAHTS Stranded mRNA seq Library Prep Kit for Illumina v2 Vazyme Biotech  China and subjected to 150bp paired end sequencing on the Illumina Novaseq 6000 platform. Trimmomatic software was employed to remove low quality reads and sequencing adapters  followed by the alignment of clean reads to the zebrafish reference genome GRCz11 v106 using STAR software. Read counts for each gene were calculated using featureCounts.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP496008", null, null, "M20-3_1.fq.gz M20-3_2.fq.gz", "fastq fastq", 6761256900.0, 22537523.0, "M20 3 1.fq.gz", "0:150 1:150", "A:1758917520;C:1603264927;G:1642146940;T:1756875822;N:51691", 150, 150, null, null, 1758917520, 1603264927, 1642146940, 1756875822, 51691, "SRX23975257", "SRS20773675", "SRA1825755", "shanghai ocean university|College of Fisheries and Life Science", "shanghai ocean university", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2024-03-18", "Adult", "Adult", "Gonad", "Reproductive System"], [26556, "SRR28370197", "SRX23975256", "SRS20773674", "SRP496008", "PRJNA1020641", "Danio rerio strain:AB strain Raw sequence reads", "PRJNA1020641", "Whole Genome Sequencing", "post different low temperature treatments for 14 days  transcriptome sequencing was conducted on ovaries to analyze the metabolic state under low temperatures.", null, null, null, null, "M20 2 1", null, "strain:not applicable|age:7|dev stage:month|collection date:2021 08 23|geo loc name:not applicable|sex:female|tissue:ovary|replicate:replicate=biological replicate 11|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA seq of ovary: adult female zebrafish", "E11", "E11", "Strand specific RNA seq libraries were prepared using the VAHTS Stranded mRNA seq Library Prep Kit for Illumina v2 Vazyme Biotech  China and subjected to 150bp paired end sequencing on the Illumina Novaseq 6000 platform. Trimmomatic software was employed to remove low quality reads and sequencing adapters  followed by the alignment of clean reads to the zebrafish reference genome GRCz11 v106 using STAR software. Read counts for each gene were calculated using featureCounts.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP496008", null, null, "M20-2_1.fq.gz M20-2_2.fq.gz", "fastq fastq", 6746623800.0, 22488746.0, "M20 2 1.fq.gz", "0:150 1:150", "A:1744481110;C:1606775717;G:1646048497;T:1749267538;N:50938", 150, 150, null, null, 1744481110, 1606775717, 1646048497, 1749267538, 50938, "SRX23975256", "SRS20773674", "SRA1825755", "shanghai ocean university|College of Fisheries and Life Science", "shanghai ocean university", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2024-03-18", "Adult", "Adult", "Gonad", "Reproductive System"], [26557, "SRR28370198", "SRX23975255", "SRS20773673", "SRP496008", "PRJNA1020641", "Danio rerio strain:AB strain Raw sequence reads", "PRJNA1020641", "Whole Genome Sequencing", "post different low temperature treatments for 14 days  transcriptome sequencing was conducted on ovaries to analyze the metabolic state under low temperatures.", null, null, null, null, "H27 1 1", null, "strain:not applicable|age:7|dev stage:month|collection date:2021 08 23|geo loc name:not applicable|sex:female|tissue:ovary|replicate:replicate=biological replicate 1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA seq of ovary: adult female zebrafish", "E1", "E1", "Strand specific RNA seq libraries were prepared using the VAHTS Stranded mRNA seq Library Prep Kit for Illumina v2 Vazyme Biotech  China and subjected to 150bp paired end sequencing on the Illumina Novaseq 6000 platform. Trimmomatic software was employed to remove low quality reads and sequencing adapters  followed by the alignment of clean reads to the zebrafish reference genome GRCz11 v106 using STAR software. 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"PRJNA1056454", "miR214 regulates sex determination through gsdf in zebrafish", "PRJNA1056454", "Other", "Sex determination is a variable and complex mechanism  yet it can be found all over the plant and animal kingdoms. It creates two morphological different outcomes from one and the same species. Our work demonstrates the involvement of a non coding RNA in this process for the teleost Danio rerio. Although we were not able to understand the full mechanism behind it  we can provide a piece of the puzzle  which once again highlights the complexity and flexibility of sex determination.", null, null, "CrisprCas9 generated using 2 sgRNAs  >F3  crossed toTgvasa:eGFP", "miR214 PaternalKO gonads 31dpf rep2", "miR214 PaternalKO gonads 31dpf rep2", null, "strain:mz 06de  tgvasa:eGFP|isolate:not applicable|breed:TUE|cultivar:not applicable|ecotype:not applicable|age:27 dpf|dev stage:27 dpf|sex:not applicable|tissue:gonads|parental ko:PaternalKO|biological replicate:rep2|number of gonads:9 gonads|birth date:21.09.2018|collection date:not applicable|genotype:homozygous mutation for miR 214 from a heterozygous female crossed to a homozygous male|geo loc name:not applicable|growth protocol:standard|phenotype:n1|sample type:tissue|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "= xlfn.CONCAT\"mrna sequencing of zebrafish: \" A11", "miR214 PaternalKO gonads 31dpf rep2", "miR214 PaternalKO gonads 31dpf rep2", "NGS library prep was performed with Illumina's TruSeq stranded mRNA LT Sample Prep Kit following Illuminas standard protocol Part # 15031047 Rev  E. Libraries were prepared with a starting amount of 193 ng and amplified in 13 PCR cycles  Libraries were profiled in a DNA 1000 Chip on a 2100 Bioanalyzer Agilent technologies and quantified using the Qubit dsDNA HS Assay Kit  in a Qubit 2 0 Fluorometer Life technologies. All 16 samples were pooled in equimolar ratio and sequenced on 1 NextSeq 500 Highoutput FC  SR for 1 x 84 cycles plus 7 cycles for the index read.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "SINGLE", "ILLUMINA", "NextSeq 500", null, "SRP479907", null, null, "miR214_PaternalKO_gonads_31dpf_rep2.fastq.gz", "fastq", 2327996832.0, 27714248.0, "miR214 PaternalKO gonads 31dpf rep2.fastq.gz", "0:84", "A:602135965;C:552786494;G:537368428;T:635697339;N:8606", 84, null, null, null, 602135965, 552786494, 537368428, 635697339, 8606, "SRX23013778", "SRS19977075", "SRA1775014", "Rene Ketting group|Ketting Lab", "Rene Ketting group", 1, 0.95369, null, 0.06146, null, 0.69203, null, 0.47259, null, 84, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Germany", "2023-12-24", "Larval", "Larval", "Gonad", "Reproductive System"], [29260, "SRR27336918", "SRX23013777", "SRS19977074", "SRP479907", "PRJNA1056454", "miR214 regulates sex determination through gsdf in zebrafish", "PRJNA1056454", "Other", "Sex determination is a variable and complex mechanism  yet it can be found all over the plant and animal kingdoms. It creates two morphological different outcomes from one and the same species. Our work demonstrates the involvement of a non coding RNA in this process for the teleost Danio rerio. Although we were not able to understand the full mechanism behind it  we can provide a piece of the puzzle  which once again highlights the complexity and flexibility of sex determination.", null, null, "CrisprCas9 generated using 2 sgRNAs  >F3  crossed toTgvasa:eGFP", "miR214 PaternalKO gonads 31dpf rep1", "miR214 PaternalKO gonads 31dpf rep1", null, "strain:mz 06de  tgvasa:eGFP|isolate:not applicable|breed:TUE|cultivar:not applicable|ecotype:not applicable|age:27 dpf|dev stage:27 dpf|sex:not applicable|tissue:gonads|parental ko:PaternalKO|biological replicate:rep1|number of gonads:9 gonads|birth date:21.09.2018|collection date:not applicable|genotype:homozygous mutation for miR 214 from a heterozygous female crossed to a homozygous male|geo loc name:not applicable|growth protocol:standard|phenotype:n1|sample type:tissue|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "= xlfn.CONCAT\"mrna sequencing of zebrafish: \" A10", "miR214 PaternalKO gonads 31dpf rep1", "miR214 PaternalKO gonads 31dpf rep1", "NGS library prep was performed with Illumina's TruSeq stranded mRNA LT Sample Prep Kit following Illuminas standard protocol Part # 15031047 Rev  E. Libraries were prepared with a starting amount of 193 ng and amplified in 13 PCR cycles  Libraries were profiled in a DNA 1000 Chip on a 2100 Bioanalyzer Agilent technologies and quantified using the Qubit dsDNA HS Assay Kit  in a Qubit 2 0 Fluorometer Life technologies. All 16 samples were pooled in equimolar ratio and sequenced on 1 NextSeq 500 Highoutput FC  SR for 1 x 84 cycles plus 7 cycles for the index read.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "SINGLE", "ILLUMINA", "NextSeq 500", null, "SRP479907", null, null, "miR214_PaternalKO_gonads_31dpf_rep1.fastq.gz", "fastq", 2500978452.0, 29773553.0, "miR214 PaternalKO gonads 31dpf rep1.fastq.gz", "0:84", "A:646162933;C:593855971;G:578265372;T:682684551;N:9625", 84, null, null, null, 646162933, 593855971, 578265372, 682684551, 9625, "SRX23013777", "SRS19977074", "SRA1775014", "Rene Ketting group|Ketting Lab", "Rene Ketting group", 1, 0.95147, null, 0.05972, null, 0.69071, null, 0.47043, null, 84, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Germany", "2023-12-24", "Larval", "Larval", "Gonad", "Reproductive System"], [29261, "SRR27336919", "SRX23013776", "SRS19977073", "SRP479907", "PRJNA1056454", "miR214 regulates sex determination through gsdf in zebrafish", "PRJNA1056454", "Other", "Sex determination is a variable and complex mechanism  yet it can be found all over the plant and animal kingdoms. It creates two morphological different outcomes from one and the same species. Our work demonstrates the involvement of a non coding RNA in this process for the teleost Danio rerio. Although we were not able to understand the full mechanism behind it  we can provide a piece of the puzzle  which once again highlights the complexity and flexibility of sex determination.", null, null, "CrisprCas9 generated using 2 sgRNAs  >F3  crossed toTgvasa:eGFP", "miR214 maternalHET gonads 31dpf rep4", "miR214 maternalHET gonads 31dpf rep4", null, "strain:mz 06de  tgvasa:eGFP|isolate:not applicable|breed:TUE|cultivar:not applicable|ecotype:not applicable|age:27 dpf|dev stage:27 dpf|sex:not applicable|tissue:gonads|parental ko:maternalHET|biological replicate:rep4|number of gonads:7 gonads|birth date:21.09.2018|collection date:not applicable|genotype:heterozygous mutation for miR 214 from a homozygous female crossed to a heterozygous male|geo loc name:not applicable|growth protocol:standard|phenotype:n1|sample type:tissue|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "= xlfn.CONCAT\"mrna sequencing of zebrafish: \" A9", "miR214 maternalHET gonads 31dpf rep4", "miR214 maternalHET gonads 31dpf rep4", "NGS library prep was performed with Illumina's TruSeq stranded mRNA LT Sample Prep Kit following Illuminas standard protocol Part # 15031047 Rev  E. Libraries were prepared with a starting amount of 193 ng and amplified in 13 PCR cycles  Libraries were profiled in a DNA 1000 Chip on a 2100 Bioanalyzer Agilent technologies and quantified using the Qubit dsDNA HS Assay Kit  in a Qubit 2 0 Fluorometer Life technologies. All 16 samples were pooled in equimolar ratio and sequenced on 1 NextSeq 500 Highoutput FC  SR for 1 x 84 cycles plus 7 cycles for the index read.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "SINGLE", "ILLUMINA", "NextSeq 500", null, "SRP479907", null, null, "miR214_maternalHET_gonads_31dpf_rep4.fastq.gz", "fastq", 2365480908.0, 28160487.0, "miR214 maternalHET gonads 31dpf rep4.fastq.gz", "0:84", "A:613121312;C:560582165;G:549338096;T:642429834;N:9501", 84, null, null, null, 613121312, 560582165, 549338096, 642429834, 9501, "SRX23013776", "SRS19977073", "SRA1775014", "Rene Ketting group|Ketting Lab", "Rene Ketting group", 1, 0.95304, null, 0.05342, null, 0.7108, null, 0.46822, null, 84, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Germany", "2023-12-24", "Larval", "Larval", "Gonad", "Reproductive System"], [29262, "SRR27336920", "SRX23013775", "SRS19977072", "SRP479907", "PRJNA1056454", "miR214 regulates sex determination through gsdf in zebrafish", "PRJNA1056454", "Other", "Sex determination is a variable and complex mechanism  yet it can be found all over the plant and animal kingdoms. It creates two morphological different outcomes from one and the same species. Our work demonstrates the involvement of a non coding RNA in this process for the teleost Danio rerio. Although we were not able to understand the full mechanism behind it  we can provide a piece of the puzzle  which once again highlights the complexity and flexibility of sex determination.", null, null, "CrisprCas9 generated using 2 sgRNAs  >F3  crossed toTgvasa:eGFP", "miR214 maternalHET gonads 31dpf rep3", "miR214 maternalHET gonads 31dpf rep3", null, "strain:mz 06de  tgvasa:eGFP|isolate:not applicable|breed:TUE|cultivar:not applicable|ecotype:not applicable|age:27 dpf|dev stage:27 dpf|sex:not applicable|tissue:gonads|parental ko:maternalHET|biological replicate:rep3|number of gonads:7 gonads|birth date:21.09.2018|collection date:not applicable|genotype:heterozygous mutation for miR 214 from a homozygous female crossed to a heterozygous male|geo loc name:not applicable|growth protocol:standard|phenotype:n1|sample type:tissue|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "= xlfn.CONCAT\"mrna sequencing of zebrafish: \" A8", "miR214 maternalHET gonads 31dpf rep3", "miR214 maternalHET gonads 31dpf rep3", "NGS library prep was performed with Illumina's TruSeq stranded mRNA LT Sample Prep Kit following Illuminas standard protocol Part # 15031047 Rev  E. Libraries were prepared with a starting amount of 193 ng and amplified in 13 PCR cycles  Libraries were profiled in a DNA 1000 Chip on a 2100 Bioanalyzer Agilent technologies and quantified using the Qubit dsDNA HS Assay Kit  in a Qubit 2 0 Fluorometer Life technologies. All 16 samples were pooled in equimolar ratio and sequenced on 1 NextSeq 500 Highoutput FC  SR for 1 x 84 cycles plus 7 cycles for the index read.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "SINGLE", "ILLUMINA", "NextSeq 500", null, "SRP479907", null, null, "miR214_maternalHET_gonads_31dpf_rep3.fastq.gz", "fastq", 2537794644.0, 30211841.0, "miR214 maternalHET gonads 31dpf rep3.fastq.gz", "0:84", "A:658687178;C:600742464;G:585643169;T:692712057;N:9776", 84, null, null, null, 658687178, 600742464, 585643169, 692712057, 9776, "SRX23013775", "SRS19977072", "SRA1775014", "Rene Ketting group|Ketting Lab", "Rene Ketting group", 1, 0.95177, null, 0.06701, null, 0.68195, null, 0.47393, null, 84, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Germany", "2023-12-24", "Larval", "Larval", "Gonad", "Reproductive System"], [29263, "SRR27336921", "SRX23013774", "SRS19977071", "SRP479907", "PRJNA1056454", "miR214 regulates sex determination through gsdf in zebrafish", "PRJNA1056454", "Other", "Sex determination is a variable and complex mechanism  yet it can be found all over the plant and animal kingdoms. It creates two morphological different outcomes from one and the same species. Our work demonstrates the involvement of a non coding RNA in this process for the teleost Danio rerio. Although we were not able to understand the full mechanism behind it  we can provide a piece of the puzzle  which once again highlights the complexity and flexibility of sex determination.", null, null, "CrisprCas9 generated using 2 sgRNAs  >F3  crossed toTgvasa:eGFP", "miR214 maternalHET gonads 31dpf rep2", "miR214 maternalHET gonads 31dpf rep2", null, "strain:mz 06de  tgvasa:eGFP|isolate:not applicable|breed:TUE|cultivar:not applicable|ecotype:not applicable|age:27 dpf|dev stage:27 dpf|sex:not applicable|tissue:gonads|parental ko:maternalHET|biological replicate:rep2|number of gonads:7 gonads|birth date:21.09.2018|collection date:not applicable|genotype:heterozygous mutation for miR 214 from a homozygous female crossed to a heterozygous male|geo loc name:not applicable|growth protocol:standard|phenotype:n1|sample type:tissue|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "= xlfn.CONCAT\"mrna sequencing of zebrafish: \" A7", "miR214 maternalHET gonads 31dpf rep2", "miR214 maternalHET gonads 31dpf rep2", "NGS library prep was performed with Illumina's TruSeq stranded mRNA LT Sample Prep Kit following Illuminas standard protocol Part # 15031047 Rev  E. Libraries were prepared with a starting amount of 193 ng and amplified in 13 PCR cycles  Libraries were profiled in a DNA 1000 Chip on a 2100 Bioanalyzer Agilent technologies and quantified using the Qubit dsDNA HS Assay Kit  in a Qubit 2 0 Fluorometer Life technologies. All 16 samples were pooled in equimolar ratio and sequenced on 1 NextSeq 500 Highoutput FC  SR for 1 x 84 cycles plus 7 cycles for the index read.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "SINGLE", "ILLUMINA", "NextSeq 500", null, "SRP479907", null, null, "miR214_maternalHET_gonads_31dpf_rep2.fastq.gz", "fastq", 2417384424.0, 28778386.0, "miR214 maternalHET gonads 31dpf rep2.fastq.gz", "0:84", "A:625935186;C:574179061;G:560498085;T:656762980;N:9112", 84, null, null, null, 625935186, 574179061, 560498085, 656762980, 9112, "SRX23013774", "SRS19977071", "SRA1775014", "Rene Ketting group|Ketting Lab", "Rene Ketting group", 1, 0.95259, null, 0.05852, null, 0.6981, null, 0.47435, null, 84, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Germany", "2023-12-24", "Larval", "Larval", "Gonad", "Reproductive System"], [29264, "SRR27336922", "SRX23013773", "SRS19977070", "SRP479907", "PRJNA1056454", "miR214 regulates sex determination through gsdf in zebrafish", "PRJNA1056454", "Other", "Sex determination is a variable and complex mechanism  yet it can be found all over the plant and animal kingdoms. It creates two morphological different outcomes from one and the same species. Our work demonstrates the involvement of a non coding RNA in this process for the teleost Danio rerio. Although we were not able to understand the full mechanism behind it  we can provide a piece of the puzzle  which once again highlights the complexity and flexibility of sex determination.", null, null, "CrisprCas9 generated using 2 sgRNAs  >F3  crossed toTgvasa:eGFP", "miR214 maternalHET gonads 31dpf rep1", "miR214 maternalHET gonads 31dpf rep1", null, "strain:mz 06de  tgvasa:eGFP|isolate:not applicable|breed:TUE|cultivar:not applicable|ecotype:not applicable|age:27 dpf|dev stage:27 dpf|sex:not applicable|tissue:gonads|parental ko:maternalHET|biological replicate:rep1|number of gonads:7 gonads|birth date:21.09.2018|collection date:not applicable|genotype:heterozygous mutation for miR 214 from a homozygous female crossed to a heterozygous male|geo loc name:not applicable|growth protocol:standard|phenotype:n1|sample type:tissue|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "= xlfn.CONCAT\"mrna sequencing of zebrafish: \" A6", "miR214 maternalHET gonads 31dpf rep1", "miR214 maternalHET gonads 31dpf rep1", "NGS library prep was performed with Illumina's TruSeq stranded mRNA LT Sample Prep Kit following Illuminas standard protocol Part # 15031047 Rev  E. Libraries were prepared with a starting amount of 193 ng and amplified in 13 PCR cycles  Libraries were profiled in a DNA 1000 Chip on a 2100 Bioanalyzer Agilent technologies and quantified using the Qubit dsDNA HS Assay Kit  in a Qubit 2 0 Fluorometer Life technologies. All 16 samples were pooled in equimolar ratio and sequenced on 1 NextSeq 500 Highoutput FC  SR for 1 x 84 cycles plus 7 cycles for the index read.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "SINGLE", "ILLUMINA", "NextSeq 500", null, "SRP479907", null, null, "miR214_maternalHET_gonads_31dpf_rep1.fastq.gz", "fastq", 2330886180.0, 27748645.0, "miR214 maternalHET gonads 31dpf rep1.fastq.gz", "0:84", "A:601589571;C:554105326;G:542789267;T:632393158;N:8858", 84, null, null, null, 601589571, 554105326, 542789267, 632393158, 8858, "SRX23013773", "SRS19977070", "SRA1775014", "Rene Ketting group|Ketting Lab", "Rene Ketting group", 1, 0.95338, null, 0.05239, null, 0.71366, null, 0.47291, null, 84, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Germany", "2023-12-24", "Larval", "Larval", "Gonad", "Reproductive System"], [29265, "SRR27336923", "SRX23013772", "SRS19977069", "SRP479907", "PRJNA1056454", "miR214 regulates sex determination through gsdf in zebrafish", "PRJNA1056454", "Other", "Sex determination is a variable and complex mechanism  yet it can be found all over the plant and animal kingdoms. It creates two morphological different outcomes from one and the same species. Our work demonstrates the involvement of a non coding RNA in this process for the teleost Danio rerio. Although we were not able to understand the full mechanism behind it  we can provide a piece of the puzzle  which once again highlights the complexity and flexibility of sex determination.", null, null, "CrisprCas9 generated using 2 sgRNAs  >F3  crossed toTgvasa:eGFP", "miR214 maternalKO gonads 31dpf rep4", "miR214 maternalKO gonads 31dpf rep4", null, "strain:mz 06de  tgvasa:eGFP|isolate:not applicable|breed:TUE|cultivar:not applicable|ecotype:not applicable|age:27 dpf|dev stage:27 dpf|sex:not applicable|tissue:gonads|parental ko:maternalKO|biological replicate:rep4|number of gonads:8 gonads|birth date:21.09.2018|collection date:not applicable|genotype:homozygous mutation for miR 214 from a homozygous female crossed to a heterozygous male|geo loc name:not applicable|growth protocol:standard|phenotype:n1|sample type:tissue|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "= xlfn.CONCAT\"mrna sequencing of zebrafish: \" A5", "miR214 maternalKO gonads 31dpf rep4", "miR214 maternalKO gonads 31dpf rep4", "NGS library prep was performed with Illumina's TruSeq stranded mRNA LT Sample Prep Kit following Illuminas standard protocol Part # 15031047 Rev  E. Libraries were prepared with a starting amount of 193 ng and amplified in 13 PCR cycles  Libraries were profiled in a DNA 1000 Chip on a 2100 Bioanalyzer Agilent technologies and quantified using the Qubit dsDNA HS Assay Kit  in a Qubit 2 0 Fluorometer Life technologies. All 16 samples were pooled in equimolar ratio and sequenced on 1 NextSeq 500 Highoutput FC  SR for 1 x 84 cycles plus 7 cycles for the index read.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "SINGLE", "ILLUMINA", "NextSeq 500", null, "SRP479907", null, null, "miR214_maternalKO_gonads_31dpf_rep4.fastq.gz", "fastq", 2340233784.0, 27859926.0, "miR214 maternalKO gonads 31dpf rep4.fastq.gz", "0:84", "A:606735187;C:553647808;G:539865459;T:639976430;N:8900", 84, null, null, null, 606735187, 553647808, 539865459, 639976430, 8900, "SRX23013772", "SRS19977069", "SRA1775014", "Rene Ketting group|Ketting Lab", "Rene Ketting group", 1, 0.95406, null, 0.06415, null, 0.68225, null, 0.47695, null, 84, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Germany", "2023-12-24", "Larval", "Larval", "Gonad", "Reproductive System"], [29266, "SRR27336924", "SRX23013771", "SRS19977068", "SRP479907", "PRJNA1056454", "miR214 regulates sex determination through gsdf in zebrafish", "PRJNA1056454", "Other", "Sex determination is a variable and complex mechanism  yet it can be found all over the plant and animal kingdoms. It creates two morphological different outcomes from one and the same species. Our work demonstrates the involvement of a non coding RNA in this process for the teleost Danio rerio. Although we were not able to understand the full mechanism behind it  we can provide a piece of the puzzle  which once again highlights the complexity and flexibility of sex determination.", null, null, "CrisprCas9 generated using 2 sgRNAs  >F3  crossed toTgvasa:eGFP", "miR214 maternalKO gonads 31dpf rep3", "miR214 maternalKO gonads 31dpf rep3", null, "strain:mz 06de  tgvasa:eGFP|isolate:not applicable|breed:TUE|cultivar:not applicable|ecotype:not applicable|age:27 dpf|dev stage:27 dpf|sex:not applicable|tissue:gonads|parental ko:maternalKO|biological replicate:rep3|number of gonads:9 gonads|birth date:21.09.2018|collection date:not applicable|genotype:homozygous mutation for miR 214 from a homozygous female crossed to a heterozygous male|geo loc name:not applicable|growth protocol:standard|phenotype:n1|sample type:tissue|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "= xlfn.CONCAT\"mrna sequencing of zebrafish: \" A4", "miR214 maternalKO gonads 31dpf rep3", "miR214 maternalKO gonads 31dpf rep3", "NGS library prep was performed with Illumina's TruSeq stranded mRNA LT Sample Prep Kit following Illuminas standard protocol Part # 15031047 Rev  E. Libraries were prepared with a starting amount of 193 ng and amplified in 13 PCR cycles  Libraries were profiled in a DNA 1000 Chip on a 2100 Bioanalyzer Agilent technologies and quantified using the Qubit dsDNA HS Assay Kit  in a Qubit 2 0 Fluorometer Life technologies. All 16 samples were pooled in equimolar ratio and sequenced on 1 NextSeq 500 Highoutput FC  SR for 1 x 84 cycles plus 7 cycles for the index read.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "SINGLE", "ILLUMINA", "NextSeq 500", null, "SRP479907", null, null, "miR214_maternalKO_gonads_31dpf_rep3.fastq.gz", "fastq", 2058505512.0, 24506018.0, "miR214 maternalKO gonads 31dpf rep3.fastq.gz", "0:84", "A:537425814;C:485664494;G:471526682;T:563881213;N:7309", 84, null, null, null, 537425814, 485664494, 471526682, 563881213, 7309, "SRX23013771", "SRS19977068", "SRA1775014", "Rene Ketting group|Ketting Lab", "Rene Ketting group", 1, 0.95296, null, 0.06728, null, 0.69266, null, 0.47627, null, 84, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Germany", "2023-12-24", "Larval", "Larval", "Gonad", "Reproductive System"], [29267, "SRR27336925", "SRX23013770", "SRS19977067", "SRP479907", "PRJNA1056454", "miR214 regulates sex determination through gsdf in zebrafish", "PRJNA1056454", "Other", "Sex determination is a variable and complex mechanism  yet it can be found all over the plant and animal kingdoms. It creates two morphological different outcomes from one and the same species. Our work demonstrates the involvement of a non coding RNA in this process for the teleost Danio rerio. Although we were not able to understand the full mechanism behind it  we can provide a piece of the puzzle  which once again highlights the complexity and flexibility of sex determination.", null, null, "CrisprCas9 generated using 2 sgRNAs  >F3  crossed toTgvasa:eGFP", "miR214 PaternalHET gonads 31dpf rep4", "miR214 PaternalHET gonads 31dpf rep4", null, "strain:mz 06de  tgvasa:eGFP|isolate:not applicable|breed:TUE|cultivar:not applicable|ecotype:not applicable|age:27 dpf|dev stage:27 dpf|sex:not applicable|tissue:gonads|parental ko:PaternalHET|biological replicate:rep4|number of gonads:9 gonads|birth date:21.09.2018|collection date:not applicable|genotype:heterozygous mutation for miR 214 from a heterozygous female crossed to a homozygous male|geo loc name:not applicable|growth protocol:standard|phenotype:n1|sample type:tissue|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "= xlfn.CONCAT\"mrna sequencing of zebrafish: \" A17", "miR214 PaternalHET gonads 31dpf rep4", "miR214 PaternalHET gonads 31dpf rep4", "NGS library prep was performed with Illumina's TruSeq stranded mRNA LT Sample Prep Kit following Illuminas standard protocol Part # 15031047 Rev  E. Libraries were prepared with a starting amount of 193 ng and amplified in 13 PCR cycles  Libraries were profiled in a DNA 1000 Chip on a 2100 Bioanalyzer Agilent technologies and quantified using the Qubit dsDNA HS Assay Kit  in a Qubit 2 0 Fluorometer Life technologies. All 16 samples were pooled in equimolar ratio and sequenced on 1 NextSeq 500 Highoutput FC  SR for 1 x 84 cycles plus 7 cycles for the index read.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "SINGLE", "ILLUMINA", "NextSeq 500", null, "SRP479907", null, null, "miR214_PaternalHET_gonads_31dpf_rep4.fastq.gz", "fastq", 3001613328.0, 35733492.0, "miR214 PaternalHET gonads 31dpf rep4.fastq.gz", "0:84", "A:778860501;C:711155422;G:688784969;T:822801157;N:11279", 84, null, null, null, 778860501, 711155422, 688784969, 822801157, 11279, "SRX23013770", "SRS19977067", "SRA1775014", "Rene Ketting group|Ketting Lab", "Rene Ketting group", 1, 0.95361, null, 0.05653, null, 0.7051, null, 0.48382, null, 84, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Germany", "2023-12-24", "Larval", "Larval", "Gonad", "Reproductive System"], [29268, "SRR27336926", "SRX23013769", "SRS19977066", "SRP479907", "PRJNA1056454", "miR214 regulates sex determination through gsdf in zebrafish", "PRJNA1056454", "Other", "Sex determination is a variable and complex mechanism  yet it can be found all over the plant and animal kingdoms. It creates two morphological different outcomes from one and the same species. Our work demonstrates the involvement of a non coding RNA in this process for the teleost Danio rerio. Although we were not able to understand the full mechanism behind it  we can provide a piece of the puzzle  which once again highlights the complexity and flexibility of sex determination.", null, null, "CrisprCas9 generated using 2 sgRNAs  >F3  crossed toTgvasa:eGFP", "miR214 PaternalHET gonads 31dpf rep3", "miR214 PaternalHET gonads 31dpf rep3", null, "strain:mz 06de  tgvasa:eGFP|isolate:not applicable|breed:TUE|cultivar:not applicable|ecotype:not applicable|age:27 dpf|dev stage:27 dpf|sex:not applicable|tissue:gonads|parental ko:PaternalHET|biological replicate:rep3|number of gonads:9 gonads|birth date:21.09.2018|collection date:not applicable|genotype:heterozygous mutation for miR 214 from a heterozygous female crossed to a homozygous male|geo loc name:not applicable|growth protocol:standard|phenotype:n1|sample type:tissue|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "= xlfn.CONCAT\"mrna sequencing of zebrafish: \" A16", "miR214 PaternalHET gonads 31dpf rep3", "miR214 PaternalHET gonads 31dpf rep3", "NGS library prep was performed with Illumina's TruSeq stranded mRNA LT Sample Prep Kit following Illuminas standard protocol Part # 15031047 Rev  E. Libraries were prepared with a starting amount of 193 ng and amplified in 13 PCR cycles  Libraries were profiled in a DNA 1000 Chip on a 2100 Bioanalyzer Agilent technologies and quantified using the Qubit dsDNA HS Assay Kit  in a Qubit 2 0 Fluorometer Life technologies. All 16 samples were pooled in equimolar ratio and sequenced on 1 NextSeq 500 Highoutput FC  SR for 1 x 84 cycles plus 7 cycles for the index read.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "SINGLE", "ILLUMINA", "NextSeq 500", null, "SRP479907", null, null, "miR214_PaternalHET_gonads_31dpf_rep3.fastq.gz", "fastq", 3053630664.0, 36352746.0, "miR214 PaternalHET gonads 31dpf rep3.fastq.gz", "0:84", "A:785219727;C:726431662;G:713454025;T:828513556;N:11694", 84, null, null, null, 785219727, 726431662, 713454025, 828513556, 11694, "SRX23013769", "SRS19977066", "SRA1775014", "Rene Ketting group|Ketting Lab", "Rene Ketting group", 1, 0.95309, null, 0.04588, null, 0.71997, null, 0.46447, null, 84, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Germany", "2023-12-24", "Larval", "Larval", "Gonad", "Reproductive System"], [29269, "SRR27336927", "SRX23013768", "SRS19977065", "SRP479907", "PRJNA1056454", "miR214 regulates sex determination through gsdf in zebrafish", "PRJNA1056454", "Other", "Sex determination is a variable and complex mechanism  yet it can be found all over the plant and animal kingdoms. It creates two morphological different outcomes from one and the same species. Our work demonstrates the involvement of a non coding RNA in this process for the teleost Danio rerio. Although we were not able to understand the full mechanism behind it  we can provide a piece of the puzzle  which once again highlights the complexity and flexibility of sex determination.", null, null, "CrisprCas9 generated using 2 sgRNAs  >F3  crossed toTgvasa:eGFP", "miR214 PaternalHET gonads 31dpf rep2", "miR214 PaternalHET gonads 31dpf rep2", null, "strain:mz 06de  tgvasa:eGFP|isolate:not applicable|breed:TUE|cultivar:not applicable|ecotype:not applicable|age:27 dpf|dev stage:27 dpf|sex:not applicable|tissue:gonads|parental ko:PaternalHET|biological replicate:rep2|number of gonads:9 gonads|birth date:21.09.2018|collection date:not applicable|genotype:heterozygous mutation for miR 214 from a heterozygous female crossed to a homozygous male|geo loc name:not applicable|growth protocol:standard|phenotype:n1|sample type:tissue|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "= xlfn.CONCAT\"mrna sequencing of zebrafish: \" A15", "miR214 PaternalHET gonads 31dpf rep2", "miR214 PaternalHET gonads 31dpf rep2", "NGS library prep was performed with Illumina's TruSeq stranded mRNA LT Sample Prep Kit following Illuminas standard protocol Part # 15031047 Rev  E. Libraries were prepared with a starting amount of 193 ng and amplified in 13 PCR cycles  Libraries were profiled in a DNA 1000 Chip on a 2100 Bioanalyzer Agilent technologies and quantified using the Qubit dsDNA HS Assay Kit  in a Qubit 2 0 Fluorometer Life technologies. All 16 samples were pooled in equimolar ratio and sequenced on 1 NextSeq 500 Highoutput FC  SR for 1 x 84 cycles plus 7 cycles for the index read.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "SINGLE", "ILLUMINA", "NextSeq 500", null, "SRP479907", null, null, "miR214_PaternalHET_gonads_31dpf_rep2.fastq.gz", "fastq", 3063695208.0, 36472562.0, "miR214 PaternalHET gonads 31dpf rep2.fastq.gz", "0:84", "A:784924646;C:728941687;G:720847462;T:828969510;N:11903", 84, null, null, null, 784924646, 728941687, 720847462, 828969510, 11903, "SRX23013768", "SRS19977065", "SRA1775014", "Rene Ketting group|Ketting Lab", "Rene Ketting group", 1, 0.95413, null, 0.04739, null, 0.71936, null, 0.4704, null, 84, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Germany", "2023-12-24", "Larval", "Larval", "Gonad", "Reproductive System"], [29270, "SRR27336928", "SRX23013767", "SRS19977064", "SRP479907", "PRJNA1056454", "miR214 regulates sex determination through gsdf in zebrafish", "PRJNA1056454", "Other", "Sex determination is a variable and complex mechanism  yet it can be found all over the plant and animal kingdoms. It creates two morphological different outcomes from one and the same species. Our work demonstrates the involvement of a non coding RNA in this process for the teleost Danio rerio. Although we were not able to understand the full mechanism behind it  we can provide a piece of the puzzle  which once again highlights the complexity and flexibility of sex determination.", null, null, "CrisprCas9 generated using 2 sgRNAs  >F3  crossed toTgvasa:eGFP", "miR214 PaternalHET gonads 31dpf rep1", "miR214 PaternalHET gonads 31dpf rep1", null, "strain:mz 06de  tgvasa:eGFP|isolate:not applicable|breed:TUE|cultivar:not applicable|ecotype:not applicable|age:27 dpf|dev stage:27 dpf|sex:not applicable|tissue:gonads|parental ko:PaternalHET|biological replicate:rep1|number of gonads:9 gonads|birth date:21.09.2018|collection date:not applicable|genotype:heterozygous mutation for miR 214 from a heterozygous female crossed to a homozygous male|geo loc name:not applicable|growth protocol:standard|phenotype:n1|sample type:tissue|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "= xlfn.CONCAT\"mrna sequencing of zebrafish: \" A14", "miR214 PaternalHET gonads 31dpf rep1", "miR214 PaternalHET gonads 31dpf rep1", "NGS library prep was performed with Illumina's TruSeq stranded mRNA LT Sample Prep Kit following Illuminas standard protocol Part # 15031047 Rev  E. Libraries were prepared with a starting amount of 193 ng and amplified in 13 PCR cycles  Libraries were profiled in a DNA 1000 Chip on a 2100 Bioanalyzer Agilent technologies and quantified using the Qubit dsDNA HS Assay Kit  in a Qubit 2 0 Fluorometer Life technologies. All 16 samples were pooled in equimolar ratio and sequenced on 1 NextSeq 500 Highoutput FC  SR for 1 x 84 cycles plus 7 cycles for the index read.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "SINGLE", "ILLUMINA", "NextSeq 500", null, "SRP479907", null, null, "miR214_PaternalHET_gonads_31dpf_rep1.fastq.gz", "fastq", 2699154024.0, 32132786.0, "miR214 PaternalHET gonads 31dpf rep1.fastq.gz", "0:84", "A:694174797;C:642049572;G:627991562;T:734927762;N:10331", 84, null, null, null, 694174797, 642049572, 627991562, 734927762, 10331, "SRX23013767", "SRS19977064", "SRA1775014", "Rene Ketting group|Ketting Lab", "Rene Ketting group", 1, 0.95518, null, 0.05525, null, 0.70285, null, 0.47695, null, 84, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Germany", "2023-12-24", "Larval", "Larval", "Gonad", "Reproductive System"], [29271, "SRR27336929", "SRX23013766", "SRS19977063", "SRP479907", "PRJNA1056454", "miR214 regulates sex determination through gsdf in zebrafish", "PRJNA1056454", "Other", "Sex determination is a variable and complex mechanism  yet it can be found all over the plant and animal kingdoms. It creates two morphological different outcomes from one and the same species. Our work demonstrates the involvement of a non coding RNA in this process for the teleost Danio rerio. Although we were not able to understand the full mechanism behind it  we can provide a piece of the puzzle  which once again highlights the complexity and flexibility of sex determination.", null, null, "CrisprCas9 generated using 2 sgRNAs  >F3  crossed toTgvasa:eGFP", "miR214 PaternalKO gonads 31dpf rep4", "miR214 PaternalKO gonads 31dpf rep4", null, "strain:mz 06de  tgvasa:eGFP|isolate:not applicable|breed:TUE|cultivar:not applicable|ecotype:not applicable|age:27 dpf|dev stage:27 dpf|sex:not applicable|tissue:gonads|parental ko:PaternalKO|biological replicate:rep4|number of gonads:8 gonads|birth date:21.09.2018|collection date:not applicable|genotype:homozygous mutation for miR 214 from a heterozygous female crossed to a homozygous male|geo loc name:not applicable|growth protocol:standard|phenotype:n1|sample type:tissue|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "= xlfn.CONCAT\"mrna sequencing of zebrafish: \" A13", "miR214 PaternalKO gonads 31dpf rep4", "miR214 PaternalKO gonads 31dpf rep4", "NGS library prep was performed with Illumina's TruSeq stranded mRNA LT Sample Prep Kit following Illuminas standard protocol Part # 15031047 Rev  E. Libraries were prepared with a starting amount of 193 ng and amplified in 13 PCR cycles  Libraries were profiled in a DNA 1000 Chip on a 2100 Bioanalyzer Agilent technologies and quantified using the Qubit dsDNA HS Assay Kit  in a Qubit 2 0 Fluorometer Life technologies. All 16 samples were pooled in equimolar ratio and sequenced on 1 NextSeq 500 Highoutput FC  SR for 1 x 84 cycles plus 7 cycles for the index read.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "SINGLE", "ILLUMINA", "NextSeq 500", null, "SRP479907", null, null, "miR214_PaternalKO_gonads_31dpf_rep4.fastq.gz", "fastq", 2514248016.0, 29931524.0, "miR214 PaternalKO gonads 31dpf rep4.fastq.gz", "0:84", "A:648908956;C:598837726;G:580002743;T:686488678;N:9913", 84, null, null, null, 648908956, 598837726, 580002743, 686488678, 9913, "SRX23013766", "SRS19977063", "SRA1775014", "Rene Ketting group|Ketting Lab", "Rene Ketting group", 1, 0.94978, null, 0.06951, null, 0.67896, null, 0.47686, null, 84, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Germany", "2023-12-24", "Larval", "Larval", "Gonad", "Reproductive System"], [29272, "SRR27336930", "SRX23013765", "SRS19977062", "SRP479907", "PRJNA1056454", "miR214 regulates sex determination through gsdf in zebrafish", "PRJNA1056454", "Other", "Sex determination is a variable and complex mechanism  yet it can be found all over the plant and animal kingdoms. It creates two morphological different outcomes from one and the same species. Our work demonstrates the involvement of a non coding RNA in this process for the teleost Danio rerio. Although we were not able to understand the full mechanism behind it  we can provide a piece of the puzzle  which once again highlights the complexity and flexibility of sex determination.", null, null, "CrisprCas9 generated using 2 sgRNAs  >F3  crossed toTgvasa:eGFP", "miR214 PaternalKO gonads 31dpf rep3", "miR214 PaternalKO gonads 31dpf rep3", null, "strain:mz 06de  tgvasa:eGFP|isolate:not applicable|breed:TUE|cultivar:not applicable|ecotype:not applicable|age:27 dpf|dev stage:27 dpf|sex:not applicable|tissue:gonads|parental ko:PaternalKO|biological replicate:rep3|number of gonads:9 gonads|birth date:21.09.2018|collection date:not applicable|genotype:homozygous mutation for miR 214 from a heterozygous female crossed to a homozygous male|geo loc name:not applicable|growth protocol:standard|phenotype:n1|sample type:tissue|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "= xlfn.CONCAT\"mrna sequencing of zebrafish: \" A12", "miR214 PaternalKO gonads 31dpf rep3", "miR214 PaternalKO gonads 31dpf rep3", "NGS library prep was performed with Illumina's TruSeq stranded mRNA LT Sample Prep Kit following Illuminas standard protocol Part # 15031047 Rev  E. Libraries were prepared with a starting amount of 193 ng and amplified in 13 PCR cycles  Libraries were profiled in a DNA 1000 Chip on a 2100 Bioanalyzer Agilent technologies and quantified using the Qubit dsDNA HS Assay Kit  in a Qubit 2 0 Fluorometer Life technologies. All 16 samples were pooled in equimolar ratio and sequenced on 1 NextSeq 500 Highoutput FC  SR for 1 x 84 cycles plus 7 cycles for the index read.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "SINGLE", "ILLUMINA", "NextSeq 500", null, "SRP479907", null, null, "miR214_PaternalKO_gonads_31dpf_rep3.fastq.gz", "fastq", 2499529200.0, 29756300.0, "miR214 PaternalKO gonads 31dpf rep3.fastq.gz", "0:84", "A:642490769;C:597403823;G:580565141;T:679060154;N:9313", 84, null, null, null, 642490769, 597403823, 580565141, 679060154, 9313, "SRX23013765", "SRS19977062", "SRA1775014", "Rene Ketting group|Ketting Lab", "Rene Ketting group", 1, 0.95284, null, 0.05602, null, 0.70289, null, 0.47154, null, 84, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Germany", "2023-12-24", "Larval", "Larval", "Gonad", "Reproductive System"], [29273, "SRR27336931", "SRX23013764", "SRS19977061", "SRP479907", "PRJNA1056454", "miR214 regulates sex determination through gsdf in zebrafish", "PRJNA1056454", "Other", "Sex determination is a variable and complex mechanism  yet it can be found all over the plant and animal kingdoms. It creates two morphological different outcomes from one and the same species. Our work demonstrates the involvement of a non coding RNA in this process for the teleost Danio rerio. Although we were not able to understand the full mechanism behind it  we can provide a piece of the puzzle  which once again highlights the complexity and flexibility of sex determination.", null, null, "CrisprCas9 generated using 2 sgRNAs  >F3  crossed toTgvasa:eGFP", "miR214 maternalKO gonads 31dpf rep2", "miR214 maternalKO gonads 31dpf rep2", null, "strain:mz 06de  tgvasa:eGFP|isolate:not applicable|breed:TUE|cultivar:not applicable|ecotype:not applicable|age:27 dpf|dev stage:27 dpf|sex:not applicable|tissue:gonads|parental ko:maternalKO|biological replicate:rep2|number of gonads:9 gonads|birth date:21.09.2018|collection date:not applicable|genotype:homozygous mutation for miR 214 from a homozygous female crossed to a heterozygous male|geo loc name:not applicable|growth protocol:standard|phenotype:n1|sample type:tissue|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "= xlfn.CONCAT\"mrna sequencing of zebrafish: \" A3", "miR214 maternalKO gonads 31dpf rep2", "miR214 maternalKO gonads 31dpf rep2", "NGS library prep was performed with Illumina's TruSeq stranded mRNA LT Sample Prep Kit following Illuminas standard protocol Part # 15031047 Rev  E. Libraries were prepared with a starting amount of 193 ng and amplified in 13 PCR cycles  Libraries were profiled in a DNA 1000 Chip on a 2100 Bioanalyzer Agilent technologies and quantified using the Qubit dsDNA HS Assay Kit  in a Qubit 2 0 Fluorometer Life technologies. All 16 samples were pooled in equimolar ratio and sequenced on 1 NextSeq 500 Highoutput FC  SR for 1 x 84 cycles plus 7 cycles for the index read.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "SINGLE", "ILLUMINA", "NextSeq 500", null, "SRP479907", null, null, "miR214_maternalKO_gonads_31dpf_rep2.fastq.gz", "fastq", 2529742404.0, 30115981.0, "miR214 maternalKO gonads 31dpf rep2.fastq.gz", "0:84", "A:652091166;C:600932059;G:589100617;T:687608862;N:9700", 84, null, null, null, 652091166, 600932059, 589100617, 687608862, 9700, "SRX23013764", "SRS19977061", "SRA1775014", "Rene Ketting group|Ketting Lab", "Rene Ketting group", 1, 0.95498, null, 0.05524, null, 0.70366, null, 0.47319, null, 84, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Germany", "2023-12-24", "Larval", "Larval", "Gonad", "Reproductive System"], [29274, "SRR27336932", "SRX23013763", "SRS19977060", "SRP479907", "PRJNA1056454", "miR214 regulates sex determination through gsdf in zebrafish", "PRJNA1056454", "Other", "Sex determination is a variable and complex mechanism  yet it can be found all over the plant and animal kingdoms. It creates two morphological different outcomes from one and the same species. Our work demonstrates the involvement of a non coding RNA in this process for the teleost Danio rerio. Although we were not able to understand the full mechanism behind it  we can provide a piece of the puzzle  which once again highlights the complexity and flexibility of sex determination.", null, null, "CrisprCas9 generated using 2 sgRNAs  >F3  crossed toTgvasa:eGFP", "miR214 maternalKO gonads 31dpf rep1", "miR214 maternalKO gonads 31dpf rep1", null, "strain:mz 06de  tgvasa:eGFP|isolate:not applicable|breed:TUE|cultivar:not applicable|ecotype:not applicable|age:27 dpf|dev stage:27 dpf|sex:not applicable|tissue:gonads|parental ko:maternalKO|biological replicate:rep1|number of gonads:9 gonads|birth date:21.09.2018|collection date:not applicable|genotype:homozygous mutation for miR 214 from a homozygous female crossed to a heterozygous male|geo loc name:not applicable|growth protocol:standard|phenotype:n1|sample type:tissue|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "= xlfn.CONCAT\"mrna sequencing of zebrafish: \" A2", "miR214 maternalKO gonads 31dpf rep1", "miR214 maternalKO gonads 31dpf rep1", "NGS library prep was performed with Illumina's TruSeq stranded mRNA LT Sample Prep Kit following Illuminas standard protocol Part # 15031047 Rev  E. Libraries were prepared with a starting amount of 193 ng and amplified in 13 PCR cycles  Libraries were profiled in a DNA 1000 Chip on a 2100 Bioanalyzer Agilent technologies and quantified using the Qubit dsDNA HS Assay Kit  in a Qubit 2 0 Fluorometer Life technologies. All 16 samples were pooled in equimolar ratio and sequenced on 1 NextSeq 500 Highoutput FC  SR for 1 x 84 cycles plus 7 cycles for the index read.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "SINGLE", "ILLUMINA", "NextSeq 500", null, "SRP479907", null, null, "miR214_maternalKO_gonads_31dpf_rep1.fastq.gz", "fastq", 2310878136.0, 27510454.0, "miR214 maternalKO gonads 31dpf rep1.fastq.gz", "0:84", "A:598541924;C:547875391;G:533137786;T:631314274;N:8761", 84, null, null, null, 598541924, 547875391, 533137786, 631314274, 8761, "SRX23013763", "SRS19977060", "SRA1775014", "Rene Ketting group|Ketting Lab", "Rene Ketting group", 1, 0.95279, null, 0.06446, null, 0.68757, null, 0.4758, null, 84, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Germany", "2023-12-24", "Larval", "Larval", "Gonad", "Reproductive System"], [29720, "SRR27485665", "SRX23156884", "SRS20107305", "SRP482074", "PRJNA1061456", "tRAM seq: tRNA abundance and modification analysis during zebrafish embryo development", "PRJNA1061456", "Other", null, null, null, null, null, "ovary R3", null, "strain:TLAB fish|isolate:NA|breed:cross of zebrafish AB and the natural variant TL Tupfel Longfin|cultivar:NA|ecotype:NA|dev stage:adult|collection date:2022|geo loc name:Austria|sex:female|tissue:ovary|replicate:3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "mRNA seq of zebrafish: ovary  rep4", "EV06008", "EV06008", "RNA was extracted with Trizol and processed with QuantSeq three prime mRNA Seq Library Prep Kit FWD for Illumina Lexogen. 500 ng total RNA per sample. Single indexed QuantSeq libraries were QC checked on a Bioanalyzer 2100 Agilent using a High Sensitivity DNA Kit for correct insert size and quantified using Qubit dsDNA HS Assay Invitrogen. Pooled libraries were sequenced on a NextSeq500 instrument Illumina in 1x75bp single end sequencing mode", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "SINGLE", "ILLUMINA", "NextSeq 500", null, "SRP482074", null, null, "EV06008.R1.fastq.gz", "fastq", 585955025.0, 7769646.0, "EV06008.R1.fastq.gz", "0:75.42", "A:167210227;C:106735220;G:128095616;T:183896888;N:17074", 75, null, null, null, 167210227, 106735220, 128095616, 183896888, 17074, "SRX23156884", "SRS20107305", "SRA1783314", "Medical University of Vienna|Cell and Developmental Biology", "Medical University of Vienna", 1, 0.85544, null, 0.05096, null, 0.78441, null, 0.53007, null, 76, null, "B", null, "usable mapping rate", "illumina", "nextseq", "3prime", "poly_a", "lexogen", "bulk", "unknown", "unknown", null, "Austria", "2024-01-11", "Adult", "Adult", "Gonad", "Reproductive System"], [29731, "SRR27485676", "SRX23156873", "SRS20107294", "SRP482074", "PRJNA1061456", "tRAM seq: tRNA abundance and modification analysis during zebrafish embryo development", "PRJNA1061456", "Other", null, null, null, null, null, "ovary R2", null, "strain:TLAB fish|isolate:NA|breed:cross of zebrafish AB and the natural variant TL Tupfel Longfin|cultivar:NA|ecotype:NA|dev stage:adult|collection date:2022|geo loc name:Austria|sex:female|tissue:ovary|replicate:2|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "mRNA seq of zebrafish: ovary  rep4", "EV06001", "EV06001", "RNA was extracted with Trizol and processed with QuantSeq three prime mRNA Seq Library Prep Kit FWD for Illumina Lexogen. 500 ng total RNA per sample. Single indexed QuantSeq libraries were QC checked on a Bioanalyzer 2100 Agilent using a High Sensitivity DNA Kit for correct insert size and quantified using Qubit dsDNA HS Assay Invitrogen. 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"L1EGC150005--ZF_C2.R1.raw.fastq.gz L1EGC150005--ZF_C2.R2.raw.fastq.gz", "fastq fastq", 14593396812.0, 48322506.0, "L1EGC150005  ZF C2.R1.raw.fastq.gz", "0:151 1:151", "A:3717343233;C:3516949638;G:3746868538;T:3612192707;N:42696", 151, 151, null, null, 3717343233, 3516949638, 3746868538, 3612192707, 42696, "SRX28302708", "SRS24645575", "SRA2108762", "South China Normal University|School of environment", "South China Normal University", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2025-04-09", "Adult", "Adult", "Gonad", "Reproductive System"], [35807, "SRR33037167", "SRX28302707", "SRS24645574", "SRP577302", "PRJNA1247234", "Danio rerio Raw sequence reads", "PRJNA1247234", "Whole Genome Sequencing", "normal RNA sequencing of gonads in Zebrafish Danio rerio", null, null, null, null, "ZF Ctrl 1", null, "breed:zebrafish|age:adult|collection date:2022 01 05|geo loc name:not collected|sex:female|tissue:gonad|replicate:replicate=biological replicate 1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA seq of zebrafish", "zebra1", "zebra1", "normal RNA seq of zebrafish", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "PAIRED", "ILLUMINA", "Illumina NovaSeq X Plus", null, "SRP577302", null, null, "L1EGC150004--ZF_C1.R1.raw.fastq.gz L1EGC150004--ZF_C1.R2.raw.fastq.gz", "fastq fastq", 12184189396.0, 40344998.0, "L1EGC150004  ZF C1.R1.raw.fastq.gz", "0:151 1:151", "A:3187725727;C:2884827858;G:3037733456;T:3073866757;N:35598", 151, 151, null, null, 3187725727, 2884827858, 3037733456, 3073866757, 35598, "SRX28302707", "SRS24645574", "SRA2108762", "South China Normal University|School of environment", "South China Normal University", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2025-04-09", "Adult", "Adult", "Gonad", "Reproductive System"], [50911, "SRR8377211", "SRX5187246", "SRS4193648", "SRP174644", "PRJNA512103", "Danio rerio amh mutant RNA Seq", "PRJNA512103", "Other", "For 21dpf and 35dpf juvenile zebrafish  trunk tissue was isolated by removing the anterior of the fish from just posterior of the pectoral fin and removing the caudal peduncle posterior to the anus. For adults  ovary and testis was dissected separately from other tissues. Total RNA was extracted using the Ribopure Kit Thermo Fisher  and polyA mRNA was enriched using Dynabeads Thermo Fisher. We constructed indexed  strand specific cDNA sequencing libraries using the NEXTflex\u2122 qRNA seq kit BIOO Scientific. Libraries were normalized to 2.3nM  multiplexed  and evaluated by quantitative real time PCR using the Kapa Library Quantification Kit Kapa Biosystems. One lane of paired end 100 base pair bp sequencing was performed on an Illumina HiSeq 4000.", null, "pubmed:31399485", null, null, "Mut test8", null, "strain:AB|age:adult|sex:male|tissue:testis|biomaterial provider:Zebrafish Facility at the University of Oregon|collected by:Yilin Yan|replicate:biological replicate 8 mutant testis|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Danio rerio amh mutant RNA seq", "Mut test8", "Mut test8", "Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of the animals by removing the anterior of the fish from just posterior of the pectoral fin and removing the caudal peduncle posterior to the anus. Trunks were individually homogenized in 200ul Trizol. Total RNA was extracted following Amores  2011. Total RNA was enriched for mRNA using Dynabeads Oligodt25 ThermoFisher. We constructed indexed  strand specific cDNA sequencing libraries using the NEXTflex qRNA seq kit BIOO Scientific. Library concentrations were quantified using a Qubit fluorometer Life Technologies  normalized to a concentration of 2.3nM  and multiplexed. Prior to sequencing  we further evaluated the quality of the multiplexed library by quantitative real time PCR using the Kapa Library Quantification Kit Kapa Biosystems. One lane of paired end 100 base pair bp sequencing was performed on an Illumina HiSeq 4000.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "SRP174644", null, null, "Mut_test8_R1.fq.gz Mut_test8_R2.fq.gz", "fastq fastq", 921784580.0, 4563290.0, "Mut test8 R1.fq.gz", "0:101 1:101", "A:236359939;C:216070172;G:219949129;T:247094952;N:2310388", 101, 101, null, null, 236359939, 216070172, 219949129, 247094952, 2310388, "SRX5187246", "SRS4193648", "SRA827573", "University of Oregon|Biology", "University of Oregon", 2, 0.78301, 0.81043, 0.13524, 0.11867, 0.65389, 0.65723, 0.42789, 0.5722, 101, 101, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "United States", "2019-09-06", "Adult", "Adult", "Gonad", "Reproductive System"], [50912, "SRR8377212", "SRX5187245", "SRS4193647", "SRP174644", "PRJNA512103", "Danio rerio amh mutant RNA Seq", "PRJNA512103", "Other", "For 21dpf and 35dpf juvenile zebrafish  trunk tissue was isolated by removing the anterior of the fish from just posterior of the pectoral fin and removing the caudal peduncle posterior to the anus. For adults  ovary and testis was dissected separately from other tissues. Total RNA was extracted using the Ribopure Kit Thermo Fisher  and polyA mRNA was enriched using Dynabeads Thermo Fisher. We constructed indexed  strand specific cDNA sequencing libraries using the NEXTflex\u2122 qRNA seq kit BIOO Scientific. Libraries were normalized to 2.3nM  multiplexed  and evaluated by quantitative real time PCR using the Kapa Library Quantification Kit Kapa Biosystems. One lane of paired end 100 base pair bp sequencing was performed on an Illumina HiSeq 4000.", null, "pubmed:31399485", null, null, "Mut test7", null, "strain:AB|age:adult|sex:male|tissue:testis|biomaterial provider:Zebrafish Facility at the University of Oregon|collected by:Yilin Yan|replicate:biological replicate 7 mutant testis|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Danio rerio amh mutant RNA seq", "Mut test7", "Mut test7", "Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of the animals by removing the anterior of the fish from just posterior of the pectoral fin and removing the caudal peduncle posterior to the anus. Trunks were individually homogenized in 200ul Trizol. Total RNA was extracted following Amores  2011. Total RNA was enriched for mRNA using Dynabeads Oligodt25 ThermoFisher. We constructed indexed  strand specific cDNA sequencing libraries using the NEXTflex qRNA seq kit BIOO Scientific. Library concentrations were quantified using a Qubit fluorometer Life Technologies  normalized to a concentration of 2.3nM  and multiplexed. Prior to sequencing  we further evaluated the quality of the multiplexed library by quantitative real time PCR using the Kapa Library Quantification Kit Kapa Biosystems. One lane of paired end 100 base pair bp sequencing was performed on an Illumina HiSeq 4000.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "SRP174644", null, null, "Mut_test7_R1.fq.gz Mut_test7_R2.fq.gz", "fastq fastq", 792655676.0, 3924038.0, "Mut test7 R1.fq.gz", "0:101 1:101", "A:207148130;C:182677007;G:184824169;T:216019509;N:1986861", 101, 101, null, null, 207148130, 182677007, 184824169, 216019509, 1986861, "SRX5187245", "SRS4193647", "SRA827573", "University of Oregon|Biology", "University of Oregon", 2, 0.77877, 0.79829, 0.17788, 0.15761, 0.65494, 0.66062, 0.42697, 0.58549, 101, 101, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "United States", "2019-09-06", "Adult", "Adult", "Gonad", "Reproductive System"], [50913, "SRR8377213", "SRX5187244", "SRS4193646", "SRP174644", "PRJNA512103", "Danio rerio amh mutant RNA Seq", "PRJNA512103", "Other", "For 21dpf and 35dpf juvenile zebrafish  trunk tissue was isolated by removing the anterior of the fish from just posterior of the pectoral fin and removing the caudal peduncle posterior to the anus. For adults  ovary and testis was dissected separately from other tissues. Total RNA was extracted using the Ribopure Kit Thermo Fisher  and polyA mRNA was enriched using Dynabeads Thermo Fisher. We constructed indexed  strand specific cDNA sequencing libraries using the NEXTflex\u2122 qRNA seq kit BIOO Scientific. Libraries were normalized to 2.3nM  multiplexed  and evaluated by quantitative real time PCR using the Kapa Library Quantification Kit Kapa Biosystems. One lane of paired end 100 base pair bp sequencing was performed on an Illumina HiSeq 4000.", null, "pubmed:31399485", null, null, "Mut test6", null, "strain:AB|age:adult|sex:male|tissue:testis|biomaterial provider:Zebrafish Facility at the University of Oregon|collected by:Yilin Yan|replicate:biological replicate 6 mutant testis|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Danio rerio amh mutant RNA seq", "Mut test6", "Mut test6", "Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of the animals by removing the anterior of the fish from just posterior of the pectoral fin and removing the caudal peduncle posterior to the anus. Trunks were individually homogenized in 200ul Trizol. Total RNA was extracted following Amores  2011. Total RNA was enriched for mRNA using Dynabeads Oligodt25 ThermoFisher. We constructed indexed  strand specific cDNA sequencing libraries using the NEXTflex qRNA seq kit BIOO Scientific. Library concentrations were quantified using a Qubit fluorometer Life Technologies  normalized to a concentration of 2.3nM  and multiplexed. Prior to sequencing  we further evaluated the quality of the multiplexed library by quantitative real time PCR using the Kapa Library Quantification Kit Kapa Biosystems. One lane of paired end 100 base pair bp sequencing was performed on an Illumina HiSeq 4000.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "SRP174644", null, null, "Mut_test6_R1.fq.gz Mut_test6_R2.fq.gz", "fastq fastq", 995777584.0, 4929592.0, "Mut test6 R1.fq.gz", "0:101 1:101", "A:247361000;C:240902425;G:246039325;T:258988854;N:2485980", 101, 101, null, null, 247361000, 240902425, 246039325, 258988854, 2485980, "SRX5187244", "SRS4193646", "SRA827573", "University of Oregon|Biology", "University of Oregon", 2, 0.80856, 0.8345, 0.11378, 0.08191, 0.69069, 0.69445, 0.41432, 0.59146, 101, 101, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "United States", "2019-09-06", "Adult", "Adult", "Gonad", "Reproductive System"], [50914, "SRR8377214", "SRX5187243", "SRS4193645", "SRP174644", "PRJNA512103", "Danio rerio amh mutant RNA Seq", "PRJNA512103", "Other", "For 21dpf and 35dpf juvenile zebrafish  trunk tissue was isolated by removing the anterior of the fish from just posterior of the pectoral fin and removing the caudal peduncle posterior to the anus. For adults  ovary and testis was dissected separately from other tissues. Total RNA was extracted using the Ribopure Kit Thermo Fisher  and polyA mRNA was enriched using Dynabeads Thermo Fisher. We constructed indexed  strand specific cDNA sequencing libraries using the NEXTflex\u2122 qRNA seq kit BIOO Scientific. Libraries were normalized to 2.3nM  multiplexed  and evaluated by quantitative real time PCR using the Kapa Library Quantification Kit Kapa Biosystems. One lane of paired end 100 base pair bp sequencing was performed on an Illumina HiSeq 4000.", null, "pubmed:31399485", null, null, "Mut test5", null, "strain:AB|age:adult|sex:male|tissue:testis|biomaterial provider:Zebrafish Facility at the University of Oregon|collected by:Yilin Yan|replicate:biological replicate 5 mutant testis|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Danio rerio amh mutant RNA seq", "Mut test5", "Mut test5", "Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of the animals by removing the anterior of the fish from just posterior of the pectoral fin and removing the caudal peduncle posterior to the anus. Trunks were individually homogenized in 200ul Trizol. Total RNA was extracted following Amores  2011. Total RNA was enriched for mRNA using Dynabeads Oligodt25 ThermoFisher. We constructed indexed  strand specific cDNA sequencing libraries using the NEXTflex qRNA seq kit BIOO Scientific. Library concentrations were quantified using a Qubit fluorometer Life Technologies  normalized to a concentration of 2.3nM  and multiplexed. Prior to sequencing  we further evaluated the quality of the multiplexed library by quantitative real time PCR using the Kapa Library Quantification Kit Kapa Biosystems. One lane of paired end 100 base pair bp sequencing was performed on an Illumina HiSeq 4000.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "SRP174644", null, null, "Mut_test5_R1.fq.gz Mut_test5_R2.fq.gz", "fastq fastq", 946685524.0, 4686562.0, "Mut test5 R1.fq.gz", "0:101 1:101", "A:244003750;C:220224385;G:224804319;T:255278005;N:2375065", 101, 101, null, null, 244003750, 220224385, 224804319, 255278005, 2375065, "SRX5187243", "SRS4193645", "SRA827573", "University of Oregon|Biology", "University of Oregon", 2, 0.77946, 0.80143, 0.13073, 0.11879, 0.64707, 0.65119, 0.43756, 0.57742, 101, 101, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "United States", "2019-09-06", "Adult", "Adult", "Gonad", "Reproductive System"], [50927, "SRR8377227", "SRX5187230", "SRS4193632", "SRP174644", "PRJNA512103", "Danio rerio amh mutant RNA Seq", "PRJNA512103", "Other", "For 21dpf and 35dpf juvenile zebrafish  trunk tissue was isolated by removing the anterior of the fish from just posterior of the pectoral fin and removing the caudal peduncle posterior to the anus. For adults  ovary and testis was dissected separately from other tissues. Total RNA was extracted using the Ribopure Kit Thermo Fisher  and polyA mRNA was enriched using Dynabeads Thermo Fisher. We constructed indexed  strand specific cDNA sequencing libraries using the NEXTflex\u2122 qRNA seq kit BIOO Scientific. Libraries were normalized to 2.3nM  multiplexed  and evaluated by quantitative real time PCR using the Kapa Library Quantification Kit Kapa Biosystems. One lane of paired end 100 base pair bp sequencing was performed on an Illumina HiSeq 4000.", null, "pubmed:31399485", null, null, "Mut ovar3", null, "strain:AB|age:adult|sex:female|tissue:ovary|biomaterial provider:Zebrafish Facility at the University of Oregon|collected by:Yilin Yan|replicate:biological replicate 3 mutant ovary|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Danio rerio amh mutant RNA seq", "Mut ovar3", "Mut ovar3", "Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of the animals by removing the anterior of the fish from just posterior of the pectoral fin and removing the caudal peduncle posterior to the anus. Trunks were individually homogenized in 200ul Trizol. Total RNA was extracted following {Amores  2011 #69}. Total RNA was enriched for mRNA using Dynabeads Oligodt25 ThermoFisher. We constructed indexed  strand specific cDNA sequencing libraries using the NEXTflex qRNA seq kit BIOO Scientific. Library concentrations were quantified using a Qubit fluorometer Life Technologies  normalized to a concentration of 2.3nM  and multiplexed. Prior to sequencing  we further evaluated the quality of the multiplexed library by quantitative real time PCR using the Kapa Library Quantification Kit Kapa Biosystems. One lane of paired end 100 base pair bp sequencing was performed on an Illumina HiSeq 4000.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "SRP174644", null, null, "Mut_ovar3_R1.fq.gz Mut_ovar3_R2.fq.gz", "fastq fastq", 2540566322.0, 12577061.0, "Mut ovar3 R1.fq.gz", "0:101 1:101", "A:642328988;C:595153938;G:613795728;T:682917257;N:6370411", 101, 101, null, null, 642328988, 595153938, 613795728, 682917257, 6370411, "SRX5187230", "SRS4193632", "SRA827573", "University of Oregon|Biology", "University of Oregon", 2, 0.8031, 0.82038, 0.02205, 0.01952, 0.76027, 0.76445, 0.44826, 0.52398, 101, 101, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "United States", "2019-09-06", "Adult", "Adult", "Gonad", "Reproductive System"], [50928, "SRR8377228", "SRX5187229", "SRS4193631", "SRP174644", "PRJNA512103", "Danio rerio amh mutant RNA Seq", "PRJNA512103", "Other", "For 21dpf and 35dpf juvenile zebrafish  trunk tissue was isolated by removing the anterior of the fish from just posterior of the pectoral fin and removing the caudal peduncle posterior to the anus. For adults  ovary and testis was dissected separately from other tissues. Total RNA was extracted using the Ribopure Kit Thermo Fisher  and polyA mRNA was enriched using Dynabeads Thermo Fisher. We constructed indexed  strand specific cDNA sequencing libraries using the NEXTflex\u2122 qRNA seq kit BIOO Scientific. Libraries were normalized to 2.3nM  multiplexed  and evaluated by quantitative real time PCR using the Kapa Library Quantification Kit Kapa Biosystems. One lane of paired end 100 base pair bp sequencing was performed on an Illumina HiSeq 4000.", null, "pubmed:31399485", null, null, "Mut ovar4", null, "strain:AB|age:adult|sex:female|tissue:ovary|biomaterial provider:Zebrafish Facility at the University of Oregon|collected by:Yilin Yan|replicate:biological replicate 4 mutant ovary|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Danio rerio amh mutant RNA seq", "Mut ovar4", "Mut ovar4", "Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of the animals by removing the anterior of the fish from just posterior of the pectoral fin and removing the caudal peduncle posterior to the anus. Trunks were individually homogenized in 200ul Trizol. Total RNA was extracted following Amores  2011. Total RNA was enriched for mRNA using Dynabeads Oligodt25 ThermoFisher. We constructed indexed  strand specific cDNA sequencing libraries using the NEXTflex qRNA seq kit BIOO Scientific. Library concentrations were quantified using a Qubit fluorometer Life Technologies  normalized to a concentration of 2.3nM  and multiplexed. Prior to sequencing  we further evaluated the quality of the multiplexed library by quantitative real time PCR using the Kapa Library Quantification Kit Kapa Biosystems. One lane of paired end 100 base pair bp sequencing was performed on an Illumina HiSeq 4000.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "SRP174644", null, null, "Mut_ovar4_R1.fq.gz Mut_ovar4_R2.fq.gz", "fastq fastq", 954221336.0, 4723868.0, "Mut ovar4 R1.fq.gz", "0:101 1:101", "A:240656530;C:224543348;G:233050024;T:253576315;N:2395119", 101, 101, null, null, 240656530, 224543348, 233050024, 253576315, 2395119, "SRX5187229", "SRS4193631", "SRA827573", "University of Oregon|Biology", "University of Oregon", 2, 0.79917, 0.81767, 0.02406, 0.02019, 0.76079, 0.76587, 0.4452, 0.52439, 101, 101, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "United States", "2019-09-06", "Adult", "Adult", "Gonad", "Reproductive System"], [50929, "SRR8377229", "SRX5187228", "SRS4193630", "SRP174644", "PRJNA512103", "Danio rerio amh mutant RNA Seq", "PRJNA512103", "Other", "For 21dpf and 35dpf juvenile zebrafish  trunk tissue was isolated by removing the anterior of the fish from just posterior of the pectoral fin and removing the caudal peduncle posterior to the anus. For adults  ovary and testis was dissected separately from other tissues. Total RNA was extracted using the Ribopure Kit Thermo Fisher  and polyA mRNA was enriched using Dynabeads Thermo Fisher. We constructed indexed  strand specific cDNA sequencing libraries using the NEXTflex\u2122 qRNA seq kit BIOO Scientific. Libraries were normalized to 2.3nM  multiplexed  and evaluated by quantitative real time PCR using the Kapa Library Quantification Kit Kapa Biosystems. One lane of paired end 100 base pair bp sequencing was performed on an Illumina HiSeq 4000.", null, "pubmed:31399485", null, null, "Mut ovar1", null, "strain:AB|age:adult|sex:female|tissue:ovary|biomaterial provider:Zebrafish Facility at the University of Oregon|collected by:Yilin Yan|replicate:biological replicate 1 mutant ovary|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Danio rerio amh mutant RNA seq", "Mut ovar1", "Mut ovar1", "Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of the animals by removing the anterior of the fish from just posterior of the pectoral fin and removing the caudal peduncle posterior to the anus. Trunks were individually homogenized in 200ul Trizol. Total RNA was extracted following {Amores  2011 #69}. Total RNA was enriched for mRNA using Dynabeads Oligodt25 ThermoFisher. We constructed indexed  strand specific cDNA sequencing libraries using the NEXTflex qRNA seq kit BIOO Scientific. Library concentrations were quantified using a Qubit fluorometer Life Technologies  normalized to a concentration of 2.3nM  and multiplexed. Prior to sequencing  we further evaluated the quality of the multiplexed library by quantitative real time PCR using the Kapa Library Quantification Kit Kapa Biosystems. One lane of paired end 100 base pair bp sequencing was performed on an Illumina HiSeq 4000.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "SRP174644", null, null, "Mut_ovar1_R2.fq.gz Mut_ovar1_R1.fq.gz", "fastq fastq", 1261230632.0, 6243716.0, "Mut ovar1 R1.fq.gz", "0:101 1:101", "A:319192722;C:298433558;G:306822515;T:333627695;N:3154142", 101, 101, null, null, 319192722, 298433558, 306822515, 333627695, 3154142, "SRX5187228", "SRS4193630", "SRA827573", "University of Oregon|Biology", "University of Oregon", 2, 0.78419, 0.8175, 0.03057, 0.02643, 0.76518, 0.7681, 0.43612, 0.52079, 101, 101, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "United States", "2018-12-28", "Adult", "Adult", "Gonad", "Reproductive System"], [50930, "SRR8377230", "SRX5187227", "SRS4193629", "SRP174644", "PRJNA512103", "Danio rerio amh mutant RNA Seq", "PRJNA512103", "Other", "For 21dpf and 35dpf juvenile zebrafish  trunk tissue was isolated by removing the anterior of the fish from just posterior of the pectoral fin and removing the caudal peduncle posterior to the anus. For adults  ovary and testis was dissected separately from other tissues. Total RNA was extracted using the Ribopure Kit Thermo Fisher  and polyA mRNA was enriched using Dynabeads Thermo Fisher. We constructed indexed  strand specific cDNA sequencing libraries using the NEXTflex\u2122 qRNA seq kit BIOO Scientific. Libraries were normalized to 2.3nM  multiplexed  and evaluated by quantitative real time PCR using the Kapa Library Quantification Kit Kapa Biosystems. One lane of paired end 100 base pair bp sequencing was performed on an Illumina HiSeq 4000.", null, "pubmed:31399485", null, null, "Mut ovar2", null, "strain:AB|age:adult|sex:female|tissue:ovary|biomaterial provider:Zebrafish Facility at the University of Oregon|collected by:Yilin Yan|replicate:biological replicate 2 mutant ovary|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Danio rerio amh mutant RNA seq", "Mut ovar2", "Mut ovar2", "Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of the animals by removing the anterior of the fish from just posterior of the pectoral fin and removing the caudal peduncle posterior to the anus. Trunks were individually homogenized in 200ul Trizol. Total RNA was extracted following {Amores  2011 #69}. Total RNA was enriched for mRNA using Dynabeads Oligodt25 ThermoFisher. We constructed indexed  strand specific cDNA sequencing libraries using the NEXTflex qRNA seq kit BIOO Scientific. Library concentrations were quantified using a Qubit fluorometer Life Technologies  normalized to a concentration of 2.3nM  and multiplexed. Prior to sequencing  we further evaluated the quality of the multiplexed library by quantitative real time PCR using the Kapa Library Quantification Kit Kapa Biosystems. One lane of paired end 100 base pair bp sequencing was performed on an Illumina HiSeq 4000.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "SRP174644", null, null, "Mut_ovar2_R1.fq.gz Mut_ovar2_R2.fq.gz", "fastq fastq", 732858626.0, 3628013.0, "Mut ovar2 R1.fq.gz", "0:101 1:101", "A:188242301;C:168675201;G:175286662;T:198821957;N:1832505", 101, 101, null, null, 188242301, 168675201, 175286662, 198821957, 1832505, "SRX5187227", "SRS4193629", "SRA827573", "University of Oregon|Biology", "University of Oregon", 2, 0.80673, 0.81536, 0.02204, 0.01994, 0.7627, 0.76566, 0.43189, 0.50864, 101, 101, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "United States", "2019-09-06", "Adult", "Adult", "Gonad", "Reproductive System"], [50946, "SRR8377246", "SRX5187211", "SRS4193613", "SRP174644", "PRJNA512103", "Danio rerio amh mutant RNA Seq", "PRJNA512103", "Other", "For 21dpf and 35dpf juvenile zebrafish  trunk tissue was isolated by removing the anterior of the fish from just posterior of the pectoral fin and removing the caudal peduncle posterior to the anus. For adults  ovary and testis was dissected separately from other tissues. Total RNA was extracted using the Ribopure Kit Thermo Fisher  and polyA mRNA was enriched using Dynabeads Thermo Fisher. We constructed indexed  strand specific cDNA sequencing libraries using the NEXTflex\u2122 qRNA seq kit BIOO Scientific. Libraries were normalized to 2.3nM  multiplexed  and evaluated by quantitative real time PCR using the Kapa Library Quantification Kit Kapa Biosystems. One lane of paired end 100 base pair bp sequencing was performed on an Illumina HiSeq 4000.", null, "pubmed:31399485", null, null, "WT ovar1", null, "strain:AB|age:adult|sex:female|tissue:ovary|biomaterial provider:Zebrafish Facility at the University of Oregon|collected by:Yilin Yan|replicate:biological replicate 1 wild type ovary|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Danio rerio amh mutant RNA seq", "WT ovar1", "WT ovar1", "Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of the animals by removing the anterior of the fish from just posterior of the pectoral fin and removing the caudal peduncle posterior to the anus. Trunks were individually homogenized in 200ul Trizol. Total RNA was extracted following Amores  2011. Total RNA was enriched for mRNA using Dynabeads Oligodt25 ThermoFisher. We constructed indexed  strand specific cDNA sequencing libraries using the NEXTflex qRNA seq kit BIOO Scientific. Library concentrations were quantified using a Qubit fluorometer Life Technologies  normalized to a concentration of 2.3nM  and multiplexed. Prior to sequencing  we further evaluated the quality of the multiplexed library by quantitative real time PCR using the Kapa Library Quantification Kit Kapa Biosystems. One lane of paired end 100 base pair bp sequencing was performed on an Illumina HiSeq 4000.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "SRP174644", null, null, "WT_ovar1_R1.fq.gz WT_ovar1_R2.fq.gz", "fastq fastq", 665032278.0, 3292239.0, "WT ovar1 R1.fq.gz", "0:101 1:101", "A:167152394;C:159953694;G:162556883;T:173695536;N:1673771", 101, 101, null, null, 167152394, 159953694, 162556883, 173695536, 1673771, "SRX5187211", "SRS4193613", "SRA827573", "University of Oregon|Biology", "University of Oregon", 2, 0.77305, 0.7925, 0.03303, 0.03049, 0.76221, 0.764, 0.44152, 0.53096, 101, 101, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "United States", "2019-09-06", "Adult", "Adult", "Gonad", "Reproductive System"], [50947, "SRR8377247", "SRX5187210", "SRS4193612", "SRP174644", "PRJNA512103", "Danio rerio amh mutant RNA Seq", "PRJNA512103", "Other", "For 21dpf and 35dpf juvenile zebrafish  trunk tissue was isolated by removing the anterior of the fish from just posterior of the pectoral fin and removing the caudal peduncle posterior to the anus. For adults  ovary and testis was dissected separately from other tissues. Total RNA was extracted using the Ribopure Kit Thermo Fisher  and polyA mRNA was enriched using Dynabeads Thermo Fisher. We constructed indexed  strand specific cDNA sequencing libraries using the NEXTflex\u2122 qRNA seq kit BIOO Scientific. Libraries were normalized to 2.3nM  multiplexed  and evaluated by quantitative real time PCR using the Kapa Library Quantification Kit Kapa Biosystems. One lane of paired end 100 base pair bp sequencing was performed on an Illumina HiSeq 4000.", null, "pubmed:31399485", null, null, "WT ovar3", null, "strain:AB|age:adult|sex:female|tissue:ovary|biomaterial provider:Zebrafish Facility at the University of Oregon|collected by:Yilin Yan|replicate:biological replicate 3 wild type ovary|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Danio rerio amh mutant RNA seq", "WT ovar3", "WT ovar3", "Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of the animals by removing the anterior of the fish from just posterior of the pectoral fin and removing the caudal peduncle posterior to the anus. Trunks were individually homogenized in 200ul Trizol. Total RNA was extracted following Amores  2011. Total RNA was enriched for mRNA using Dynabeads Oligodt25 ThermoFisher. We constructed indexed  strand specific cDNA sequencing libraries using the NEXTflex qRNA seq kit BIOO Scientific. Library concentrations were quantified using a Qubit fluorometer Life Technologies  normalized to a concentration of 2.3nM  and multiplexed. Prior to sequencing  we further evaluated the quality of the multiplexed library by quantitative real time PCR using the Kapa Library Quantification Kit Kapa Biosystems. One lane of paired end 100 base pair bp sequencing was performed on an Illumina HiSeq 4000.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "SRP174644", null, null, "WT_ovar3_R1.fq.gz WT_ovar3_R2.fq.gz", "fastq fastq", 924941234.0, 4578917.0, "WT ovar3 R1.fq.gz", "0:101 1:101", "A:231521603;C:222222435;G:226308259;T:242575970;N:2312967", 101, 101, null, null, 231521603, 222222435, 226308259, 242575970, 2312967, "SRX5187210", "SRS4193612", "SRA827573", "University of Oregon|Biology", "University of Oregon", 2, 0.78319, 0.81024, 0.03697, 0.03451, 0.75457, 0.75503, 0.44498, 0.54403, 101, 101, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "United States", "2019-09-06", "Adult", "Adult", "Gonad", "Reproductive System"], [50948, "SRR8377248", "SRX5187209", "SRS4193611", "SRP174644", "PRJNA512103", "Danio rerio amh mutant RNA Seq", "PRJNA512103", "Other", "For 21dpf and 35dpf juvenile zebrafish  trunk tissue was isolated by removing the anterior of the fish from just posterior of the pectoral fin and removing the caudal peduncle posterior to the anus. For adults  ovary and testis was dissected separately from other tissues. Total RNA was extracted using the Ribopure Kit Thermo Fisher  and polyA mRNA was enriched using Dynabeads Thermo Fisher. We constructed indexed  strand specific cDNA sequencing libraries using the NEXTflex\u2122 qRNA seq kit BIOO Scientific. Libraries were normalized to 2.3nM  multiplexed  and evaluated by quantitative real time PCR using the Kapa Library Quantification Kit Kapa Biosystems. One lane of paired end 100 base pair bp sequencing was performed on an Illumina HiSeq 4000.", null, "pubmed:31399485", null, null, "WT ovar2", null, "strain:AB|age:adult|sex:female|tissue:ovary|biomaterial provider:Zebrafish Facility at the University of Oregon|collected by:Yilin Yan|replicate:biological replicate 2 wild type ovary|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Danio rerio amh mutant RNA seq", "WT ovar2", "WT ovar2", "Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of the animals by removing the anterior of the fish from just posterior of the pectoral fin and removing the caudal peduncle posterior to the anus. Trunks were individually homogenized in 200ul Trizol. Total RNA was extracted following Amores  2011. Total RNA was enriched for mRNA using Dynabeads Oligodt25 ThermoFisher. We constructed indexed  strand specific cDNA sequencing libraries using the NEXTflex qRNA seq kit BIOO Scientific. Library concentrations were quantified using a Qubit fluorometer Life Technologies  normalized to a concentration of 2.3nM  and multiplexed. Prior to sequencing  we further evaluated the quality of the multiplexed library by quantitative real time PCR using the Kapa Library Quantification Kit Kapa Biosystems. One lane of paired end 100 base pair bp sequencing was performed on an Illumina HiSeq 4000.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "SRP174644", null, null, "WT_ovar2_R1.fq.gz WT_ovar2_R2.fq.gz", "fastq fastq", 1711791834.0, 8474217.0, "WT ovar2 R1.fq.gz", "0:101 1:101", "A:422009453;C:414454112;G:427618045;T:443430653;N:4279571", 101, 101, null, null, 422009453, 414454112, 427618045, 443430653, 4279571, "SRX5187209", "SRS4193611", "SRA827573", "University of Oregon|Biology", "University of Oregon", 2, 0.80961, 0.83405, 0.04196, 0.03648, 0.75043, 0.75509, 0.46063, 0.53569, 101, 101, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "United States", "2019-09-06", "Adult", "Adult", "Gonad", "Reproductive System"], [50949, "SRR8377249", "SRX5187208", "SRS4193610", "SRP174644", "PRJNA512103", "Danio rerio amh mutant RNA Seq", "PRJNA512103", "Other", "For 21dpf and 35dpf juvenile zebrafish  trunk tissue was isolated by removing the anterior of the fish from just posterior of the pectoral fin and removing the caudal peduncle posterior to the anus. For adults  ovary and testis was dissected separately from other tissues. Total RNA was extracted using the Ribopure Kit Thermo Fisher  and polyA mRNA was enriched using Dynabeads Thermo Fisher. We constructed indexed  strand specific cDNA sequencing libraries using the NEXTflex\u2122 qRNA seq kit BIOO Scientific. Libraries were normalized to 2.3nM  multiplexed  and evaluated by quantitative real time PCR using the Kapa Library Quantification Kit Kapa Biosystems. One lane of paired end 100 base pair bp sequencing was performed on an Illumina HiSeq 4000.", null, "pubmed:31399485", null, null, "WT test1", null, "strain:AB|age:adult|sex:male|tissue:testis|biomaterial provider:Zebrafish Facility at the University of Oregon|collected by:Yilin Yan|replicate:biological replicate 1 wild type testis|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Danio rerio amh mutant RNA seq", "WT test1", "WT test1", "Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of the animals by removing the anterior of the fish from just posterior of the pectoral fin and removing the caudal peduncle posterior to the anus. Trunks were individually homogenized in 200ul Trizol. Total RNA was extracted following Amores  2011. Total RNA was enriched for mRNA using Dynabeads Oligodt25 ThermoFisher. We constructed indexed  strand specific cDNA sequencing libraries using the NEXTflex qRNA seq kit BIOO Scientific. Library concentrations were quantified using a Qubit fluorometer Life Technologies  normalized to a concentration of 2.3nM  and multiplexed. Prior to sequencing  we further evaluated the quality of the multiplexed library by quantitative real time PCR using the Kapa Library Quantification Kit Kapa Biosystems. One lane of paired end 100 base pair bp sequencing was performed on an Illumina HiSeq 4000.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "SRP174644", null, null, "WT_test1_R2.fq.gz WT_test1_R1.fq.gz", "fastq fastq", 2303992608.0, 11405904.0, "WT test1 R1.fq.gz", "0:101 1:101", "A:567633881;C:563967121;G:572235529;T:594400255;N:5755822", 101, 101, null, null, 567633881, 563967121, 572235529, 594400255, 5755822, "SRX5187208", "SRS4193610", "SRA827573", "University of Oregon|Biology", "University of Oregon", 2, 0.80661, 0.83085, 0.17247, 0.1314, 0.69023, 0.69094, 0.39549, 0.64674, 101, 101, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "United States", "2019-09-06", "Adult", "Adult", "Gonad", "Reproductive System"], [50950, "SRR8377250", "SRX5187207", "SRS4193609", "SRP174644", "PRJNA512103", "Danio rerio amh mutant RNA Seq", "PRJNA512103", "Other", "For 21dpf and 35dpf juvenile zebrafish  trunk tissue was isolated by removing the anterior of the fish from just posterior of the pectoral fin and removing the caudal peduncle posterior to the anus. For adults  ovary and testis was dissected separately from other tissues. Total RNA was extracted using the Ribopure Kit Thermo Fisher  and polyA mRNA was enriched using Dynabeads Thermo Fisher. We constructed indexed  strand specific cDNA sequencing libraries using the NEXTflex\u2122 qRNA seq kit BIOO Scientific. Libraries were normalized to 2.3nM  multiplexed  and evaluated by quantitative real time PCR using the Kapa Library Quantification Kit Kapa Biosystems. One lane of paired end 100 base pair bp sequencing was performed on an Illumina HiSeq 4000.", null, "pubmed:31399485", null, null, "WT ovar4", null, "strain:AB|age:adult|sex:female|tissue:ovary|biomaterial provider:Zebrafish Facility at the University of Oregon|collected by:Yilin Yan|replicate:biological replicate 4 wild type ovary|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Danio rerio amh mutant RNA seq", "WT ovar4", "WT ovar4", "Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of the animals by removing the anterior of the fish from just posterior of the pectoral fin and removing the caudal peduncle posterior to the anus. Trunks were individually homogenized in 200ul Trizol. Total RNA was extracted following Amores  2011. Total RNA was enriched for mRNA using Dynabeads Oligodt25 ThermoFisher. We constructed indexed  strand specific cDNA sequencing libraries using the NEXTflex qRNA seq kit BIOO Scientific. Library concentrations were quantified using a Qubit fluorometer Life Technologies  normalized to a concentration of 2.3nM  and multiplexed. Prior to sequencing  we further evaluated the quality of the multiplexed library by quantitative real time PCR using the Kapa Library Quantification Kit Kapa Biosystems. One lane of paired end 100 base pair bp sequencing was performed on an Illumina HiSeq 4000.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "SRP174644", null, null, "WT_ovar4_R1.fq.gz WT_ovar4_R2.fq.gz", "fastq fastq", 3313457712.0, 16403256.0, "WT ovar4 R1.fq.gz", "0:101 1:101", "A:819658009;C:805343322;G:820126137;T:860008778;N:8321466", 101, 101, null, null, 819658009, 805343322, 820126137, 860008778, 8321466, "SRX5187207", "SRS4193609", "SRA827573", "University of Oregon|Biology", "University of Oregon", 2, 0.7758, 0.80453, 0.03627, 0.03327, 0.75877, 0.75923, 0.4509, 0.53523, 101, 101, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "United States", "2019-09-06", "Adult", "Adult", "Gonad", "Reproductive System"], [50951, "SRR8377251", "SRX5187206", "SRS4193608", "SRP174644", "PRJNA512103", "Danio rerio amh mutant RNA Seq", "PRJNA512103", "Other", "For 21dpf and 35dpf juvenile zebrafish  trunk tissue was isolated by removing the anterior of the fish from just posterior of the pectoral fin and removing the caudal peduncle posterior to the anus. For adults  ovary and testis was dissected separately from other tissues. Total RNA was extracted using the Ribopure Kit Thermo Fisher  and polyA mRNA was enriched using Dynabeads Thermo Fisher. We constructed indexed  strand specific cDNA sequencing libraries using the NEXTflex\u2122 qRNA seq kit BIOO Scientific. Libraries were normalized to 2.3nM  multiplexed  and evaluated by quantitative real time PCR using the Kapa Library Quantification Kit Kapa Biosystems. One lane of paired end 100 base pair bp sequencing was performed on an Illumina HiSeq 4000.", null, "pubmed:31399485", null, null, "WT test4", null, "strain:AB|age:adult|sex:male|tissue:testis|biomaterial provider:Zebrafish Facility at the University of Oregon|collected by:Yilin Yan|replicate:biological replicate 4 wild type testis|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Danio rerio amh mutant RNA seq", "WT test4", "WT test4", "Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of the animals by removing the anterior of the fish from just posterior of the pectoral fin and removing the caudal peduncle posterior to the anus. Trunks were individually homogenized in 200ul Trizol. Total RNA was extracted following Amores  2011. Total RNA was enriched for mRNA using Dynabeads Oligodt25 ThermoFisher. We constructed indexed  strand specific cDNA sequencing libraries using the NEXTflex qRNA seq kit BIOO Scientific. Library concentrations were quantified using a Qubit fluorometer Life Technologies  normalized to a concentration of 2.3nM  and multiplexed. Prior to sequencing  we further evaluated the quality of the multiplexed library by quantitative real time PCR using the Kapa Library Quantification Kit Kapa Biosystems. One lane of paired end 100 base pair bp sequencing was performed on an Illumina HiSeq 4000.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "SRP174644", null, null, "WT_test4_R1.fq.gz WT_test4_R2.fq.gz", "fastq fastq", 3904257818.0, 19328009.0, "WT test4 R1.fq.gz", "0:101 1:101", "A:975797484;C:942810562;G:953761060;T:1022122312;N:9766400", 101, 101, null, null, 975797484, 942810562, 953761060, 1022122312, 9766400, "SRX5187206", "SRS4193608", "SRA827573", "University of Oregon|Biology", "University of Oregon", 2, 0.80828, 0.82737, 0.14204, 0.11127, 0.67629, 0.67795, 0.41259, 0.58947, 101, 101, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "United States", "2019-09-06", "Adult", "Adult", "Gonad", "Reproductive System"], [50952, "SRR8377252", "SRX5187205", "SRS4193607", "SRP174644", "PRJNA512103", "Danio rerio amh mutant RNA Seq", "PRJNA512103", "Other", "For 21dpf and 35dpf juvenile zebrafish  trunk tissue was isolated by removing the anterior of the fish from just posterior of the pectoral fin and removing the caudal peduncle posterior to the anus. For adults  ovary and testis was dissected separately from other tissues. Total RNA was extracted using the Ribopure Kit Thermo Fisher  and polyA mRNA was enriched using Dynabeads Thermo Fisher. We constructed indexed  strand specific cDNA sequencing libraries using the NEXTflex\u2122 qRNA seq kit BIOO Scientific. Libraries were normalized to 2.3nM  multiplexed  and evaluated by quantitative real time PCR using the Kapa Library Quantification Kit Kapa Biosystems. One lane of paired end 100 base pair bp sequencing was performed on an Illumina HiSeq 4000.", null, "pubmed:31399485", null, null, "WT test2", null, "strain:AB|age:adult|sex:male|tissue:testis|biomaterial provider:Zebrafish Facility at the University of Oregon|collected by:Yilin Yan|replicate:biological replicate 2 wild type testis|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Danio rerio amh mutant RNA seq", "WT test2", "WT test2", "Zebrafish were euthanized in Tricaine. We isolated gonad containing trunk of the animals by removing the anterior of the fish from just posterior of the pectoral fin and removing the caudal peduncle posterior to the anus. Trunks were individually homogenized in 200ul Trizol. Total RNA was extracted following Amores  2011. Total RNA was enriched for mRNA using Dynabeads Oligodt25 ThermoFisher. We constructed indexed  strand specific cDNA sequencing libraries using the NEXTflex qRNA seq kit BIOO Scientific. Library concentrations were quantified using a Qubit fluorometer Life Technologies  normalized to a concentration of 2.3nM  and multiplexed. Prior to sequencing  we further evaluated the quality of the multiplexed library by quantitative real time PCR using the Kapa Library Quantification Kit Kapa Biosystems. One lane of paired end 100 base pair bp sequencing was performed on an Illumina HiSeq 4000.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "SRP174644", null, null, "WT_test2_R1.fq.gz WT_test2_R2.fq.gz", "fastq fastq", 1018330278.0, 5041239.0, "WT test2 R1.fq.gz", "0:101 1:101", "A:248547473;C:252682009;G:254659891;T:259887061;N:2553844", 101, 101, null, null, 248547473, 252682009, 254659891, 259887061, 2553844, "SRX5187205", "SRS4193607", "SRA827573", "University of Oregon|Biology", "University of Oregon", 2, 0.81265, 0.83613, 0.19103, 0.13656, 0.69493, 0.69495, 0.37369, 0.66738, 101, 101, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "United States", "2019-09-06", "Adult", "Adult", "Gonad", "Reproductive System"], [69479, "SRR18731940", "SRX14832179", "SRS12587950", "SRP369810", "PRJNA825753", "Zebrafish ovarian transcriptome sequencing", "PRJNA825753", "Other", "1 The transcriptome sequencing of nine samples was completed  and a total of 63.66 Gb Clean Data was obtained  and the Clean Data of each sample reached 6.14 Gb and above  and the percentage of Q30 bases was 91.64 % and above.2 The Clean Reads of each sample were compared with the designated reference genome for sequence alignment  and the alignment efficiency was 92.04% or more.3 Functional annotation was performed for all genes  including comparison with NR [1]  Swiss Prot [2]  KEGG [3]  COG [4]  KOG [5]  GO [6] and Pfam databases  and annotation results for a total of 26929 genes were obtained.4 Based on the comparison results  the libraries were evaluated.5 Based on the comparison results  gene expression analysis was performed. Based on the expression of genes in different samples  differentially expressed genes were identified  and functional annotation and enrichment analysis were performed.", null, null, null, null, "Zebrafish Ovaries", null, "strain:not applicable|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:6 Months|dev stage:adult fish|sex:female|tissue:Ovaries|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Adult zebrafish ovary", "20220412 9", "20220412 9", "materials and methods", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP369810", null, null, "GPR19-11-Rescue-3_BKDL202619368-1a-N711-AK415_1.fq.gz GPR19-11-Rescue-3_BKDL202619368-1a-N711-AK415_2.fq.gz", "fastq fastq", 7830567600.0, 26101892.0, "GPR19 11 Rescue 3 BKDL202619368 1a N711 AK415 1.fq.gz", "0:150 1:150", "A:2091186346;C:1821842935;G:1856665826;T:2060841839;N:30654", 150, 150, null, null, 2091186346, 1821842935, 1856665826, 2060841839, 30654, "SRX14832179", "SRS12587950", "SRA1402797", "zhejiang ocean university|school of fishery", "zhejiang ocean university", 2, 0.94642, 0.94446, 0.03159, 0.031, 0.73977, 0.74101, 0.49671, 0.49184, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2022-04-12", "Adult", "Adult", "Gonad", "Reproductive System"], [69480, "SRR18731941", "SRX14832178", "SRS12587950", "SRP369810", "PRJNA825753", "Zebrafish ovarian transcriptome sequencing", "PRJNA825753", "Other", "1 The transcriptome sequencing of nine samples was completed  and a total of 63.66 Gb Clean Data was obtained  and the Clean Data of each sample reached 6.14 Gb and above  and the percentage of Q30 bases was 91.64 % and above.2 The Clean Reads of each sample were compared with the designated reference genome for sequence alignment  and the alignment efficiency was 92.04% or more.3 Functional annotation was performed for all genes  including comparison with NR [1]  Swiss Prot [2]  KEGG [3]  COG [4]  KOG [5]  GO [6] and Pfam databases  and annotation results for a total of 26929 genes were obtained.4 Based on the comparison results  the libraries were evaluated.5 Based on the comparison results  gene expression analysis was performed. Based on the expression of genes in different samples  differentially expressed genes were identified  and functional annotation and enrichment analysis were performed.", null, null, null, null, "Zebrafish Ovaries", null, "strain:not applicable|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:6 Months|dev stage:adult fish|sex:female|tissue:Ovaries|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Adult zebrafish ovary", "20220412 8", "20220412 8", "materials and methods", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP369810", null, null, "GPR19-11-Rescue-2_BKDL202619368-1a-N710-AK416_1.fq.gz GPR19-11-Rescue-2_BKDL202619368-1a-N710-AK416_2.fq.gz", "fastq fastq", 6295515300.0, 20985051.0, "GPR19 11 Rescue 2 BKDL202619368 1a N710 AK416 1.fq.gz", "0:150 1:150", "A:1677101982;C:1466375952;G:1498052845;T:1653959638;N:24883", 150, 150, null, null, 1677101982, 1466375952, 1498052845, 1653959638, 24883, "SRX14832178", "SRS12587950", "SRA1402797", "zhejiang ocean university|school of fishery", "zhejiang ocean university", 2, 0.94529, 0.94401, 0.0307, 0.03031, 0.73963, 0.74126, 0.49539, 0.49777, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2022-04-12", "Adult", "Adult", "Gonad", "Reproductive System"], [69481, "SRR18731942", "SRX14832177", "SRS12587950", "SRP369810", "PRJNA825753", "Zebrafish ovarian transcriptome sequencing", "PRJNA825753", "Other", "1 The transcriptome sequencing of nine samples was completed  and a total of 63.66 Gb Clean Data was obtained  and the Clean Data of each sample reached 6.14 Gb and above  and the percentage of Q30 bases was 91.64 % and above.2 The Clean Reads of each sample were compared with the designated reference genome for sequence alignment  and the alignment efficiency was 92.04% or more.3 Functional annotation was performed for all genes  including comparison with NR [1]  Swiss Prot [2]  KEGG [3]  COG [4]  KOG [5]  GO [6] and Pfam databases  and annotation results for a total of 26929 genes were obtained.4 Based on the comparison results  the libraries were evaluated.5 Based on the comparison results  gene expression analysis was performed. Based on the expression of genes in different samples  differentially expressed genes were identified  and functional annotation and enrichment analysis were performed.", null, null, null, null, "Zebrafish Ovaries", null, "strain:not applicable|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:6 Months|dev stage:adult fish|sex:female|tissue:Ovaries|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Adult zebrafish ovary", "20220412 7", "20220412 7", "materials and methods", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP369810", null, null, "GPR19-11-Rescue-1_BKDL202619368-1a-N707-AK417_1.fq.gz GPR19-11-Rescue-1_BKDL202619368-1a-N707-AK417_2.fq.gz", "fastq fastq", 6879986100.0, 22933287.0, "GPR19 11 Rescue 1 BKDL202619368 1a N707 AK417 1.fq.gz", "0:150 1:150", "A:1836018740;C:1600279556;G:1633403531;T:1810257249;N:27024", 150, 150, null, null, 1836018740, 1600279556, 1633403531, 1810257249, 27024, "SRX14832177", "SRS12587950", "SRA1402797", "zhejiang ocean university|school of fishery", "zhejiang ocean university", 2, 0.94532, 0.94398, 0.03079, 0.03047, 0.74077, 0.7417, 0.49403, 0.49651, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2022-04-12", "Adult", "Adult", "Gonad", "Reproductive System"], [69482, "SRR18731943", "SRX14832176", "SRS12587950", "SRP369810", "PRJNA825753", "Zebrafish ovarian transcriptome sequencing", "PRJNA825753", "Other", "1 The transcriptome sequencing of nine samples was completed  and a total of 63.66 Gb Clean Data was obtained  and the Clean Data of each sample reached 6.14 Gb and above  and the percentage of Q30 bases was 91.64 % and above.2 The Clean Reads of each sample were compared with the designated reference genome for sequence alignment  and the alignment efficiency was 92.04% or more.3 Functional annotation was performed for all genes  including comparison with NR [1]  Swiss Prot [2]  KEGG [3]  COG [4]  KOG [5]  GO [6] and Pfam databases  and annotation results for a total of 26929 genes were obtained.4 Based on the comparison results  the libraries were evaluated.5 Based on the comparison results  gene expression analysis was performed. Based on the expression of genes in different samples  differentially expressed genes were identified  and functional annotation and enrichment analysis were performed.", null, null, null, null, "Zebrafish Ovaries", null, "strain:not applicable|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:6 Months|dev stage:adult fish|sex:female|tissue:Ovaries|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Adult zebrafish ovary", "20220412 6", "20220412 6", "materials and methods", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP369810", null, null, "GPR19-11-Treantment-3_BKDL202619368-1a-N706-N508_1.fq.gz GPR19-11-Treantment-3_BKDL202619368-1a-N706-N508_2.fq.gz", "fastq fastq", 7311039900.0, 24370133.0, "GPR19 11 Treantment 3 BKDL202619368 1a N706 N508 1.fq.gz", "0:150 1:150", "A:1956990026;C:1696385126;G:1732778037;T:1924857608;N:29103", 150, 150, null, null, 1956990026, 1696385126, 1732778037, 1924857608, 29103, "SRX14832176", "SRS12587950", "SRA1402797", "zhejiang ocean university|school of fishery", "zhejiang ocean university", 2, 0.94368, 0.94282, 0.02754, 0.02729, 0.74663, 0.74811, 0.49702, 0.4955, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2022-04-12", "Adult", "Adult", "Gonad", "Reproductive System"], [69483, "SRR18731944", "SRX14832175", "SRS12587950", "SRP369810", "PRJNA825753", "Zebrafish ovarian transcriptome sequencing", "PRJNA825753", "Other", "1 The transcriptome sequencing of nine samples was completed  and a total of 63.66 Gb Clean Data was obtained  and the Clean Data of each sample reached 6.14 Gb and above  and the percentage of Q30 bases was 91.64 % and above.2 The Clean Reads of each sample were compared with the designated reference genome for sequence alignment  and the alignment efficiency was 92.04% or more.3 Functional annotation was performed for all genes  including comparison with NR [1]  Swiss Prot [2]  KEGG [3]  COG [4]  KOG [5]  GO [6] and Pfam databases  and annotation results for a total of 26929 genes were obtained.4 Based on the comparison results  the libraries were evaluated.5 Based on the comparison results  gene expression analysis was performed. Based on the expression of genes in different samples  differentially expressed genes were identified  and functional annotation and enrichment analysis were performed.", null, null, null, null, "Zebrafish Ovaries", null, "strain:not applicable|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:6 Months|dev stage:adult fish|sex:female|tissue:Ovaries|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Adult zebrafish ovary", "20220412 5", "20220412 5", "materials and methods", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP369810", null, null, "GPR19-11-Treantment-2_BKDL202619368-1a-N705-N507_1.fq.gz GPR19-11-Treantment-2_BKDL202619368-1a-N705-N507_2.fq.gz", "fastq fastq", 7339647900.0, 24465493.0, "GPR19 11 Treantment 2 BKDL202619368 1a N705 N507 1.fq.gz", "0:150 1:150", "A:1968354751;C:1700662595;G:1735948919;T:1934652282;N:29353", 150, 150, null, null, 1968354751, 1700662595, 1735948919, 1934652282, 29353, "SRX14832175", "SRS12587950", "SRA1402797", "zhejiang ocean university|school of fishery", "zhejiang ocean university", 2, 0.94395, 0.94269, 0.02732, 0.02717, 0.74789, 0.74937, 0.49089, 0.49494, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2022-04-12", "Adult", "Adult", "Gonad", "Reproductive System"], [69484, "SRR18731945", "SRX14832174", "SRS12587950", "SRP369810", "PRJNA825753", "Zebrafish ovarian transcriptome sequencing", "PRJNA825753", "Other", "1 The transcriptome sequencing of nine samples was completed  and a total of 63.66 Gb Clean Data was obtained  and the Clean Data of each sample reached 6.14 Gb and above  and the percentage of Q30 bases was 91.64 % and above.2 The Clean Reads of each sample were compared with the designated reference genome for sequence alignment  and the alignment efficiency was 92.04% or more.3 Functional annotation was performed for all genes  including comparison with NR [1]  Swiss Prot [2]  KEGG [3]  COG [4]  KOG [5]  GO [6] and Pfam databases  and annotation results for a total of 26929 genes were obtained.4 Based on the comparison results  the libraries were evaluated.5 Based on the comparison results  gene expression analysis was performed. Based on the expression of genes in different samples  differentially expressed genes were identified  and functional annotation and enrichment analysis were performed.", null, null, null, null, "Zebrafish Ovaries", null, "strain:not applicable|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:6 Months|dev stage:adult fish|sex:female|tissue:Ovaries|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Adult zebrafish ovary", "20220412 4", "20220412 4", "materials and methods", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP369810", null, null, "GPR19-11-Treantment-1_BKDL202619368-1a-N704-N506_1.fq.gz GPR19-11-Treantment-1_BKDL202619368-1a-N704-N506_2.fq.gz", "fastq fastq", 6671009100.0, 22236697.0, "GPR19 11 Treantment 1 BKDL202619368 1a N704 N506 1.fq.gz", "0:150 1:150", "A:1783546565;C:1548532134;G:1583055468;T:1755848772;N:26161", 150, 150, null, null, 1783546565, 1548532134, 1583055468, 1755848772, 26161, "SRX14832174", "SRS12587950", "SRA1402797", "zhejiang ocean university|school of fishery", "zhejiang ocean university", 2, 0.94438, 0.94175, 0.02733, 0.02674, 0.7487, 0.75035, 0.49731, 0.49703, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2022-04-12", "Adult", "Adult", "Gonad", "Reproductive System"], [69485, "SRR18731946", "SRX14832173", "SRS12587950", "SRP369810", "PRJNA825753", "Zebrafish ovarian transcriptome sequencing", "PRJNA825753", "Other", "1 The transcriptome sequencing of nine samples was completed  and a total of 63.66 Gb Clean Data was obtained  and the Clean Data of each sample reached 6.14 Gb and above  and the percentage of Q30 bases was 91.64 % and above.2 The Clean Reads of each sample were compared with the designated reference genome for sequence alignment  and the alignment efficiency was 92.04% or more.3 Functional annotation was performed for all genes  including comparison with NR [1]  Swiss Prot [2]  KEGG [3]  COG [4]  KOG [5]  GO [6] and Pfam databases  and annotation results for a total of 26929 genes were obtained.4 Based on the comparison results  the libraries were evaluated.5 Based on the comparison results  gene expression analysis was performed. Based on the expression of genes in different samples  differentially expressed genes were identified  and functional annotation and enrichment analysis were performed.", null, null, null, null, "Zebrafish Ovaries", null, "strain:not applicable|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:6 Months|dev stage:adult fish|sex:female|tissue:Ovaries|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Adult zebrafish ovary", "20220412 3", "20220412 3", "materials and methods", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP369810", null, null, "GPR19-11-Control-3_BKDL202619368-1a-N703-N505_1.fq.gz GPR19-11-Control-3_BKDL202619368-1a-N703-N505_2.fq.gz", "fastq fastq", 7449711300.0, 24832371.0, "GPR19 11 Control 3 BKDL202619368 1a N703 N505 1.fq.gz", "0:150 1:150", "A:1983055279;C:1737773499;G:1771296356;T:1957556812;N:29354", 150, 150, null, null, 1983055279, 1737773499, 1771296356, 1957556812, 29354, "SRX14832173", "SRS12587950", "SRA1402797", "zhejiang ocean university|school of fishery", "zhejiang ocean university", 2, 0.94654, 0.9438, 0.03145, 0.03141, 0.73541, 0.73746, 0.4947, 0.49294, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2022-04-12", "Adult", "Adult", "Gonad", "Reproductive System"], [69486, "SRR18731947", "SRX14832172", "SRS12587950", "SRP369810", "PRJNA825753", "Zebrafish ovarian transcriptome sequencing", "PRJNA825753", "Other", "1 The transcriptome sequencing of nine samples was completed  and a total of 63.66 Gb Clean Data was obtained  and the Clean Data of each sample reached 6.14 Gb and above  and the percentage of Q30 bases was 91.64 % and above.2 The Clean Reads of each sample were compared with the designated reference genome for sequence alignment  and the alignment efficiency was 92.04% or more.3 Functional annotation was performed for all genes  including comparison with NR [1]  Swiss Prot [2]  KEGG [3]  COG [4]  KOG [5]  GO [6] and Pfam databases  and annotation results for a total of 26929 genes were obtained.4 Based on the comparison results  the libraries were evaluated.5 Based on the comparison results  gene expression analysis was performed. Based on the expression of genes in different samples  differentially expressed genes were identified  and functional annotation and enrichment analysis were performed.", null, null, null, null, "Zebrafish Ovaries", null, "strain:not applicable|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:6 Months|dev stage:adult fish|sex:female|tissue:Ovaries|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Adult zebrafish ovary", "20220412 2", "20220412 2", "materials and methods", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP369810", null, null, "GPR19-11-Control-2_BKDL202619368-1a-N702-N503_1.fq.gz GPR19-11-Control-2_BKDL202619368-1a-N702-N503_2.fq.gz", "fastq fastq", 7841586000.0, 26138620.0, "GPR19 11 Control 2 BKDL202619368 1a N702 N503 1.fq.gz", "0:150 1:150", "A:2087468135;C:1826719181;G:1866580166;T:2060787674;N:30844", 150, 150, null, null, 2087468135, 1826719181, 1866580166, 2060787674, 30844, "SRX14832172", "SRS12587950", "SRA1402797", "zhejiang ocean university|school of fishery", "zhejiang ocean university", 2, 0.94595, 0.94469, 0.03066, 0.03015, 0.73507, 0.73681, 0.49237, 0.49222, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2022-04-12", "Adult", "Adult", "Gonad", "Reproductive System"], [69487, "SRR18731948", "SRX14832171", "SRS12587950", "SRP369810", "PRJNA825753", "Zebrafish ovarian transcriptome sequencing", "PRJNA825753", "Other", "1 The transcriptome sequencing of nine samples was completed  and a total of 63.66 Gb Clean Data was obtained  and the Clean Data of each sample reached 6.14 Gb and above  and the percentage of Q30 bases was 91.64 % and above.2 The Clean Reads of each sample were compared with the designated reference genome for sequence alignment  and the alignment efficiency was 92.04% or more.3 Functional annotation was performed for all genes  including comparison with NR [1]  Swiss Prot [2]  KEGG [3]  COG [4]  KOG [5]  GO [6] and Pfam databases  and annotation results for a total of 26929 genes were obtained.4 Based on the comparison results  the libraries were evaluated.5 Based on the comparison results  gene expression analysis was performed. Based on the expression of genes in different samples  differentially expressed genes were identified  and functional annotation and enrichment analysis were performed.", null, null, null, null, "Zebrafish Ovaries", null, "strain:not applicable|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:6 Months|dev stage:adult fish|sex:female|tissue:Ovaries|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Adult zebrafish ovary", "20220412 1", "20220412 1", "materials and methods", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP369810", null, null, "GPR19-11-Control-1_BKDL202619368-1a-N701-N502_1.fq.gz GPR19-11-Control-1_BKDL202619368-1a-N701-N502_2.fq.gz", "fastq fastq", 7595890500.0, 25319635.0, "GPR19 11 Control 1 BKDL202619368 1a N701 N502 1.fq.gz", "0:150 1:150", "A:2021828878;C:1767048142;G:1812243705;T:1994739951;N:29824", 150, 150, null, 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