{"database": "metadata", "table": "run_metadata", "is_view": false, "human_description_en": "where experiment.library_selection = \"CAGE\", experiment.library_source = \"TRANSCRIPTOMIC\" and experiment.library_strategy = \"RNA-Seq\"", "rows": [[48305, "SRR10368285", "SRX7076279", "SRS5592164", "SRP145615", "PRJNA471311", "Gene expression analysis of jam3b mutant embryos", "GSE114416", "Transcriptome Analysis", "Junctional adhesion molecule 3 Jam3 is a cell adhesion molecule that is involved in cell cell interaction  cell migration  and cell polarization in many tissue including the epithelium  endothelium  and hematopoietic cells. Although Jam3 is functionally relevant for both hematopoietic and vascular endothelial cells  the role of Jam3 in hematopoietic or vascular development is still unknown. In this study  a zebrafish jam3b mutant line  jam3b sa37  was used for the cap analysis gene expression CAGE sequencing to identify genes that are involved in hematopoietic and/or vascular development in the zebrafish embryo. Overall design: Whole embryos mRNA profiles of wild type or jam3b sa37 embryos at 12 or 16 hpf were generated by CAGE sequencing using Illumina NextSeq500.", null, null, null, "sa37 16hpf 2", "GSM4144779", null, "source name:whole embryo|tissue:whole embryo|age:16 hpf sa37", "sa37 16hpf 2", "Illumina RTA software was used for base calling. Sequenced CAGE tags were mapped to the full genome sequences for Danio rerio danRer10 using the Burrows Wheeler Aligner BWA and HiSAT2 software. Tags per million TPM were calculated using the CAGEr package of Bioconductor in R ver. 3.3.3 with the minimum TPM value of 0.5. 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Although Jam3 is functionally relevant for both hematopoietic and vascular endothelial cells  the role of Jam3 in hematopoietic or vascular development is still unknown. In this study  a zebrafish jam3b mutant line  jam3b sa37  was used for the cap analysis gene expression CAGE sequencing to identify genes that are involved in hematopoietic and/or vascular development in the zebrafish embryo. Overall design: Whole embryos mRNA profiles of wild type or jam3b sa37 embryos at 12 or 16 hpf were generated by CAGE sequencing using Illumina NextSeq500.", null, null, null, "WT 12hpf", "GSM3141415", null, "source name:whole embryo|tissue:whole embryo|age:12 hpf type", "WT 12hpf", "Illumina RTA software was used for base calling. Sequenced CAGE tags were mapped to the full genome sequences for Danio rerio danRer10 using the Burrows Wheeler Aligner BWA and HiSAT2 software. Tags per million TPM were calculated using the CAGEr package of Bioconductor in R ver. 3.3.3 with the minimum TPM value of 0.5. Genome build: danRer10 Supplementary files format and content: Tab delimited text files include tags per million TPM values for each sample.", "whole embryo", null, "Total RNAs were extracted from staged embryos using RNeasy Mini Kit QIAGEN. 2\u03bcg of total RNAs from embryos were used for synthesis of the cDNA using a Library Preparation kit DNAFORM. five primecaps of RNAs are biotinylated post reverse transcription. RNA cDNA hybirds were then captured by streptavidin conjugated magnetic beads. The cDNAs were released from RNAs and ligated with the 5\u2032 linker  which includes the three nucleotide barcodes  followed by 3\u2032 linker ligation. Second strand synthesis was performed using the nAnT iCAGE second primer  creating the final double stranded DNA product. 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Here  we systematically investigate the epigenomic and transcriptomic landscape of TEs across eleven adult zebrafish tissues using multidimensional sequencing data. We find that TEs contribute substantially to a diverse array of regulatory elements in the zebrafish genome and that 37% of TEs are positioned in active regulatory states in adult zebrafish tissues. We identify TE subfamilies enriched in highly specific regulatory elements among different tissues. We use transcript assembly to discover TE derived transcriptional units expressed in a tissue specific manner. Finally  we show that novel TE derived promoters can initiate tissue specific transcription of alternative isoforms. 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Here  we systematically investigate the epigenomic and transcriptomic landscape of TEs across eleven adult zebrafish tissues using multidimensional sequencing data. We find that TEs contribute substantially to a diverse array of regulatory elements in the zebrafish genome and that 37% of TEs are positioned in active regulatory states in adult zebrafish tissues. We identify TE subfamilies enriched in highly specific regulatory elements among different tissues. We use transcript assembly to discover TE derived transcriptional units expressed in a tissue specific manner. Finally  we show that novel TE derived promoters can initiate tissue specific transcription of alternative isoforms. 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Here  we systematically investigate the epigenomic and transcriptomic landscape of TEs across eleven adult zebrafish tissues using multidimensional sequencing data. We find that TEs contribute substantially to a diverse array of regulatory elements in the zebrafish genome and that 37% of TEs are positioned in active regulatory states in adult zebrafish tissues. We identify TE subfamilies enriched in highly specific regulatory elements among different tissues. We use transcript assembly to discover TE derived transcriptional units expressed in a tissue specific manner. Finally  we show that novel TE derived promoters can initiate tissue specific transcription of alternative isoforms. 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