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Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>", "DRP003977", "Illumina HiSeq 3000 sequencing of SAMD00182223", null, null, null, 2533282452.0, 70368957.0, "DRR189380", "0:36", "A:602595083;C:585466052;G:616064286;T:729086926;N:70105", 36, null, null, null, 602595083, 585466052, 616064286, 729086926, 70105, "DRX179845", "DRS200419", "DRA008858", "NIG|National Institute of Genetics (Japan)", "National Institute of Genetics (Japan)", 1, 0.89412, null, 0.19005, null, 0.70816, null, 0.49843, null, 36, null, "B", null, "usable mapping rate", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "Japan", "2021-08-08", "Adult", "Adult", "Brain", "Nervous System"], [5795, "ERR1698352", "ERX1767860", "ERS1417534", "ERP018188", "PRJEB16335", "RNA seq of the zebrafish danio rerio brain post developmental exposure to 17a ethinylestradiol", "E-MTAB-5173", "Transcriptome Analysis", "17a ethinylestradiol EE2 is a synthetic estrogen commonly used as an active substance in oral contraceptives.  It is frequently found in waste water effluent and raise concern due to its persistent nature. EE2 binds to estrogen receptors with similar affinity to oestradiol and acts as one of the most potent hormone mimics found in the environment.  Estrogen is involved in many aspects of the development of the neuroendocrine system influencing both brain structure and behavior. We and others have reported a significant effect on non reproductive behaviors in adult fish and in recent studies we found that developmental exposure to EE2 resulted in an anxiogenic phenotype as adults even post a long remediation period.  In this study we aim to study possible mechanisms behind the behavior alterations of zebrafish developmentally exposed to EE2 by sequencing the whole brain transcriptome. Zebrafish embryos were exposed to 0  2.14 and 7.34 ng/L EE2 from 1 day to 80 dpf post the exposure period a remediation period of 120 days followed before the fish were sampled. 3 male brains from the control group 0 ng/L and the 2.14 ng/L group were sampled and 3 female brains from the control group 0 ng/L and 7.34 ng/L were sampled.", "ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2016 10 19|ArrayExpress:E MTAB 5173", null, "Protocols: RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich  Germany EE2 exposure. Fish exposed to 0  2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA", "IonXpressRNA 012 up 058 12", "SAMEA4518355", "School of Natural Science, Technology and Environmental Studies Sodertorn University", "ENA first public:2017 10 02|ENA last update:2016 10 19|External Id:SAMEA4518355|INSDC center alias:School of Natural Science  Technology and Environmental Studies Sodertorn University|INSDC center name:School of Natural Science  Technology and Environmental Studies Sodertorn University|INSDC first public:2017 10 02T17:05:16Z|INSDC last update:2016 10 19T13:53:59Z|INSDC status:public|Submitter Id:E MTAB 5173:IonXpressRNA 012 up 058 12|age:200|broker name:ArrayExpress|common name:zebrafish|organism part:brain|sample name:E MTAB 5173:IonXpressRNA 012 up 058 12|sex:male", null, null, null, null, null, null, null, null, "Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17\u03b1 ethinylestradiol", "E MTAB 5173:IonXpressRNA 012 up 058 12 s", "IonXpressRNA 012 up 058 12 s", "RNA seq of the zebrafish danio rerio brain post developmental exposure to 17\u03b1 ethinylestradiol", "RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich  Germany EE2 exposure. Fish exposed to 0  2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA", "Experimental Factor: male:sex|Experimental Factor: 17 ethinylestradiol:compound|Experimental Factor: 2.14:dose", "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM", "SINGLE", "ION_TORRENT", "Ion Torrent Proton", null, "ERP018188", "Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17\u00ce\u00b1 ethinylestradiol", "ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2018 11 16", "IonXpressRNA_012_up_058_12.fastq.gz", "fastq", 2535299006.0, 33956077.0, "E MTAB 5173:IonXpressRNA 012 up 058 12", "0:74.66", "A:719061985;C:581906269;G:579932234;T:654398518;N:0", 74, null, null, null, 719061985, 581906269, 579932234, 654398518, 0, "ERX1767860", "ERS1417534", "ERA739176", "School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive", "School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive", 1, 0.7343, null, 0.34119, null, 0.7359, null, 0.48406, null, 66, null, "B", null, "usable mapping rate", "ion_torrent", "ion_torrent", "full_length", "random_priming", "ribozero", "bulk", "unknown", "unknown", null, "Sweden", "2016-10-19", "Pharyngula", "Embryo", "Brain", "Nervous System"], [5796, "ERR1698351", "ERX1767859", "ERS1417533", "ERP018188", "PRJEB16335", "RNA seq of the zebrafish danio rerio brain post developmental exposure to 17a ethinylestradiol", "E-MTAB-5173", "Transcriptome Analysis", "17a ethinylestradiol EE2 is a synthetic estrogen commonly used as an active substance in oral contraceptives.  It is frequently found in waste water effluent and raise concern due to its persistent nature. EE2 binds to estrogen receptors with similar affinity to oestradiol and acts as one of the most potent hormone mimics found in the environment.  Estrogen is involved in many aspects of the development of the neuroendocrine system influencing both brain structure and behavior. We and others have reported a significant effect on non reproductive behaviors in adult fish and in recent studies we found that developmental exposure to EE2 resulted in an anxiogenic phenotype as adults even post a long remediation period.  In this study we aim to study possible mechanisms behind the behavior alterations of zebrafish developmentally exposed to EE2 by sequencing the whole brain transcriptome. Zebrafish embryos were exposed to 0  2.14 and 7.34 ng/L EE2 from 1 day to 80 dpf post the exposure period a remediation period of 120 days followed before the fish were sampled. 3 male brains from the control group 0 ng/L and the 2.14 ng/L group were sampled and 3 female brains from the control group 0 ng/L and 7.34 ng/L were sampled.", "ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2016 10 19|ArrayExpress:E MTAB 5173", null, "Protocols: RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich  Germany EE2 exposure. Fish exposed to 0  2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA", "IonXpressRNA 011 up 058 11", "SAMEA4518354", "School of Natural Science, Technology and Environmental Studies Sodertorn University", "ENA first public:2017 10 02|ENA last update:2016 10 19|External Id:SAMEA4518354|INSDC center alias:School of Natural Science  Technology and Environmental Studies Sodertorn University|INSDC center name:School of Natural Science  Technology and Environmental Studies Sodertorn University|INSDC first public:2017 10 02T17:05:16Z|INSDC last update:2016 10 19T13:53:59Z|INSDC status:public|Submitter Id:E MTAB 5173:IonXpressRNA 011 up 058 11|age:200|broker name:ArrayExpress|common name:zebrafish|organism part:brain|sample name:E MTAB 5173:IonXpressRNA 011 up 058 11|sex:male", null, null, null, null, null, null, null, null, "Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17\u03b1 ethinylestradiol", "E MTAB 5173:IonXpressRNA 011 up 058 11 s", "IonXpressRNA 011 up 058 11 s", "RNA seq of the zebrafish danio rerio brain post developmental exposure to 17\u03b1 ethinylestradiol", "RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich  Germany EE2 exposure. Fish exposed to 0  2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA", "Experimental Factor: male:sex|Experimental Factor: 17 ethinylestradiol:compound|Experimental Factor: 2.14:dose", "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM", "SINGLE", "ION_TORRENT", "Ion Torrent Proton", null, "ERP018188", "Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17\u00ce\u00b1 ethinylestradiol", "ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2018 11 16", "IonXpressRNA_011_up_058_11.fastq.gz", "fastq", 3400774471.0, 38709756.0, "E MTAB 5173:IonXpressRNA 011 up 058 11", "0:87.85", "A:948991439;C:793389977;G:792539547;T:865853508;N:0", 87, null, null, null, 948991439, 793389977, 792539547, 865853508, 0, "ERX1767859", "ERS1417533", "ERA739176", "School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive", "School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive", 1, 0.63968, null, 0.27033, null, 0.75213, null, 0.47966, null, 139, null, "B", null, "usable mapping rate", "ion_torrent", "ion_torrent", "full_length", "random_priming", "ribozero", "bulk", "unknown", "unknown", null, "Sweden", "2016-10-19", "Pharyngula", "Embryo", "Brain", "Nervous System"], [5797, "ERR1698350", "ERX1767858", "ERS1417532", "ERP018188", "PRJEB16335", "RNA seq of the zebrafish danio rerio brain post developmental exposure to 17a ethinylestradiol", "E-MTAB-5173", "Transcriptome Analysis", "17a ethinylestradiol EE2 is a synthetic estrogen commonly used as an active substance in oral contraceptives.  It is frequently found in waste water effluent and raise concern due to its persistent nature. EE2 binds to estrogen receptors with similar affinity to oestradiol and acts as one of the most potent hormone mimics found in the environment.  Estrogen is involved in many aspects of the development of the neuroendocrine system influencing both brain structure and behavior. We and others have reported a significant effect on non reproductive behaviors in adult fish and in recent studies we found that developmental exposure to EE2 resulted in an anxiogenic phenotype as adults even post a long remediation period.  In this study we aim to study possible mechanisms behind the behavior alterations of zebrafish developmentally exposed to EE2 by sequencing the whole brain transcriptome. Zebrafish embryos were exposed to 0  2.14 and 7.34 ng/L EE2 from 1 day to 80 dpf post the exposure period a remediation period of 120 days followed before the fish were sampled. 3 male brains from the control group 0 ng/L and the 2.14 ng/L group were sampled and 3 female brains from the control group 0 ng/L and 7.34 ng/L were sampled.", "ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2016 10 19|ArrayExpress:E MTAB 5173", null, "Protocols: RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich  Germany EE2 exposure. Fish exposed to 0  2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA", "IonXpressRNA 010 up 058 10", "SAMEA4518353", "School of Natural Science, Technology and Environmental Studies Sodertorn University", "ENA first public:2017 10 02|ENA last update:2016 10 19|External Id:SAMEA4518353|INSDC center alias:School of Natural Science  Technology and Environmental Studies Sodertorn University|INSDC center name:School of Natural Science  Technology and Environmental Studies Sodertorn University|INSDC first public:2017 10 02T17:05:16Z|INSDC last update:2016 10 19T13:53:59Z|INSDC status:public|Submitter Id:E MTAB 5173:IonXpressRNA 010 up 058 10|age:200|broker name:ArrayExpress|common name:zebrafish|organism part:brain|sample name:E MTAB 5173:IonXpressRNA 010 up 058 10|sex:male", null, null, null, null, null, null, null, null, "Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17\u03b1 ethinylestradiol", "E MTAB 5173:IonXpressRNA 010 up 058 10 s", "IonXpressRNA 010 up 058 10 s", "RNA seq of the zebrafish danio rerio brain post developmental exposure to 17\u03b1 ethinylestradiol", "RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich  Germany EE2 exposure. Fish exposed to 0  2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA", "Experimental Factor: male:sex|Experimental Factor: 17 ethinylestradiol:compound|Experimental Factor: 2.14:dose", "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM", "SINGLE", "ION_TORRENT", "Ion Torrent Proton", null, "ERP018188", "Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17\u00ce\u00b1 ethinylestradiol", "ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2018 11 16", "IonXpressRNA_010_up_058_10.fastq.gz", "fastq", 3502667998.0, 39204781.0, "E MTAB 5173:IonXpressRNA 010 up 058 10", "0:89.34", "A:976977303;C:803330255;G:808731995;T:913628445;N:0", 89, null, null, null, 976977303, 803330255, 808731995, 913628445, 0, "ERX1767858", "ERS1417532", "ERA739176", "School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive", "School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive", 1, 0.65846, null, 0.2815, null, 0.74028, null, 0.47784, null, 86, null, "B", null, "usable mapping rate", "ion_torrent", "ion_torrent", "full_length", "random_priming", "ribozero", "bulk", "unknown", "unknown", null, "Sweden", "2016-10-19", "Pharyngula", "Embryo", "Brain", "Nervous System"], [5798, "ERR1698349", "ERX1767857", "ERS1417531", "ERP018188", "PRJEB16335", "RNA seq of the zebrafish danio rerio brain post developmental exposure to 17a ethinylestradiol", "E-MTAB-5173", "Transcriptome Analysis", "17a ethinylestradiol EE2 is a synthetic estrogen commonly used as an active substance in oral contraceptives.  It is frequently found in waste water effluent and raise concern due to its persistent nature. EE2 binds to estrogen receptors with similar affinity to oestradiol and acts as one of the most potent hormone mimics found in the environment.  Estrogen is involved in many aspects of the development of the neuroendocrine system influencing both brain structure and behavior. We and others have reported a significant effect on non reproductive behaviors in adult fish and in recent studies we found that developmental exposure to EE2 resulted in an anxiogenic phenotype as adults even post a long remediation period.  In this study we aim to study possible mechanisms behind the behavior alterations of zebrafish developmentally exposed to EE2 by sequencing the whole brain transcriptome. Zebrafish embryos were exposed to 0  2.14 and 7.34 ng/L EE2 from 1 day to 80 dpf post the exposure period a remediation period of 120 days followed before the fish were sampled. 3 male brains from the control group 0 ng/L and the 2.14 ng/L group were sampled and 3 female brains from the control group 0 ng/L and 7.34 ng/L were sampled.", "ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2016 10 19|ArrayExpress:E MTAB 5173", null, "Protocols: RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich  Germany EE2 exposure. Fish exposed to 0  2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA", "IonXpressRNA 009 up 058 9", "SAMEA4518352", "School of Natural Science, Technology and Environmental Studies Sodertorn University", "ENA first public:2017 10 02|ENA last update:2016 10 19|External Id:SAMEA4518352|INSDC center alias:School of Natural Science  Technology and Environmental Studies Sodertorn University|INSDC center name:School of Natural Science  Technology and Environmental Studies Sodertorn University|INSDC first public:2017 10 02T17:05:16Z|INSDC last update:2016 10 19T13:53:59Z|INSDC status:public|Submitter Id:E MTAB 5173:IonXpressRNA 009 up 058 9|age:200|broker name:ArrayExpress|common name:zebrafish|organism part:brain|sample name:E MTAB 5173:IonXpressRNA 009 up 058 9|sex:male", null, null, null, null, null, null, null, null, "Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17\u03b1 ethinylestradiol", "E MTAB 5173:IonXpressRNA 009 up 058 9 s", "IonXpressRNA 009 up 058 9 s", "RNA seq of the zebrafish danio rerio brain post developmental exposure to 17\u03b1 ethinylestradiol", "RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich  Germany EE2 exposure. Fish exposed to 0  2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA", "Experimental Factor: male:sex|Experimental Factor: n1:compound", "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM", "SINGLE", "ION_TORRENT", "Ion Torrent Proton", null, "ERP018188", "Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17\u00ce\u00b1 ethinylestradiol", "ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2018 11 16", "IonXpressRNA_009_up_058_9.fastq.gz", "fastq", 3575088264.0, 39745481.0, "E MTAB 5173:IonXpressRNA 009 up 058 9", "0:89.95", "A:1011474126;C:809896078;G:807471974;T:946246086;N:0", 89, null, null, null, 1011474126, 809896078, 807471974, 946246086, 0, "ERX1767857", "ERS1417531", "ERA739176", "School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive", "School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive", 1, 0.65266, null, 0.30274, null, 0.75286, null, 0.48527, null, 94, null, "B", null, "usable mapping rate", "ion_torrent", "ion_torrent", "full_length", "random_priming", "ribozero", "bulk", "unknown", "unknown", null, "Sweden", "2016-10-19", "Pharyngula", "Embryo", "Brain", "Nervous System"], [5799, "ERR1698348", "ERX1767856", "ERS1417530", "ERP018188", "PRJEB16335", "RNA seq of the zebrafish danio rerio brain post developmental exposure to 17a ethinylestradiol", "E-MTAB-5173", "Transcriptome Analysis", "17a ethinylestradiol EE2 is a synthetic estrogen commonly used as an active substance in oral contraceptives.  It is frequently found in waste water effluent and raise concern due to its persistent nature. EE2 binds to estrogen receptors with similar affinity to oestradiol and acts as one of the most potent hormone mimics found in the environment.  Estrogen is involved in many aspects of the development of the neuroendocrine system influencing both brain structure and behavior. We and others have reported a significant effect on non reproductive behaviors in adult fish and in recent studies we found that developmental exposure to EE2 resulted in an anxiogenic phenotype as adults even post a long remediation period.  In this study we aim to study possible mechanisms behind the behavior alterations of zebrafish developmentally exposed to EE2 by sequencing the whole brain transcriptome. Zebrafish embryos were exposed to 0  2.14 and 7.34 ng/L EE2 from 1 day to 80 dpf post the exposure period a remediation period of 120 days followed before the fish were sampled. 3 male brains from the control group 0 ng/L and the 2.14 ng/L group were sampled and 3 female brains from the control group 0 ng/L and 7.34 ng/L were sampled.", "ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2016 10 19|ArrayExpress:E MTAB 5173", null, "Protocols: RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich  Germany EE2 exposure. Fish exposed to 0  2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA", "IonXpressRNA 008 up 058 8", "SAMEA4518351", "School of Natural Science, Technology and Environmental Studies Sodertorn University", "ENA first public:2017 10 02|ENA last update:2016 10 19|External Id:SAMEA4518351|INSDC center alias:School of Natural Science  Technology and Environmental Studies Sodertorn University|INSDC center name:School of Natural Science  Technology and Environmental Studies Sodertorn University|INSDC first public:2017 10 02T17:05:16Z|INSDC last update:2016 10 19T13:53:59Z|INSDC status:public|Submitter Id:E MTAB 5173:IonXpressRNA 008 up 058 8|age:200|broker name:ArrayExpress|common name:zebrafish|organism part:brain|sample name:E MTAB 5173:IonXpressRNA 008 up 058 8|sex:male", null, null, null, null, null, null, null, null, "Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17\u03b1 ethinylestradiol", "E MTAB 5173:IonXpressRNA 008 up 058 8 s", "IonXpressRNA 008 up 058 8 s", "RNA seq of the zebrafish danio rerio brain post developmental exposure to 17\u03b1 ethinylestradiol", "RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich  Germany EE2 exposure. Fish exposed to 0  2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA", "Experimental Factor: male:sex|Experimental Factor: n1:compound", "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM", "SINGLE", "ION_TORRENT", "Ion Torrent Proton", null, "ERP018188", "Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17\u00ce\u00b1 ethinylestradiol", "ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2018 11 16", "IonXpressRNA_008_up_058_8.fastq.gz", "fastq", 3259710114.0, 38530802.0, "E MTAB 5173:IonXpressRNA 008 up 058 8", "0:84.60", "A:916053004;C:755694888;G:751125332;T:836836890;N:0", 84, null, null, null, 916053004, 755694888, 751125332, 836836890, 0, "ERX1767856", "ERS1417530", "ERA739176", "School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive", "School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive", 1, 0.70995, null, 0.31365, null, 0.74781, null, 0.48846, null, 61, null, "B", null, "usable mapping rate", "ion_torrent", "ion_torrent", "full_length", "random_priming", "ribozero", "bulk", "unknown", "unknown", null, "Sweden", "2016-10-19", "Pharyngula", "Embryo", "Brain", "Nervous System"], [5800, "ERR1698347", "ERX1767855", "ERS1417529", "ERP018188", "PRJEB16335", "RNA seq of the zebrafish danio rerio brain post developmental exposure to 17a ethinylestradiol", "E-MTAB-5173", "Transcriptome Analysis", "17a ethinylestradiol EE2 is a synthetic estrogen commonly used as an active substance in oral contraceptives.  It is frequently found in waste water effluent and raise concern due to its persistent nature. EE2 binds to estrogen receptors with similar affinity to oestradiol and acts as one of the most potent hormone mimics found in the environment.  Estrogen is involved in many aspects of the development of the neuroendocrine system influencing both brain structure and behavior. We and others have reported a significant effect on non reproductive behaviors in adult fish and in recent studies we found that developmental exposure to EE2 resulted in an anxiogenic phenotype as adults even post a long remediation period.  In this study we aim to study possible mechanisms behind the behavior alterations of zebrafish developmentally exposed to EE2 by sequencing the whole brain transcriptome. Zebrafish embryos were exposed to 0  2.14 and 7.34 ng/L EE2 from 1 day to 80 dpf post the exposure period a remediation period of 120 days followed before the fish were sampled. 3 male brains from the control group 0 ng/L and the 2.14 ng/L group were sampled and 3 female brains from the control group 0 ng/L and 7.34 ng/L were sampled.", "ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2016 10 19|ArrayExpress:E MTAB 5173", null, "Protocols: RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich  Germany EE2 exposure. Fish exposed to 0  2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA", "IonXpressRNA 007 up 058 7", "SAMEA4518350", "School of Natural Science, Technology and Environmental Studies Sodertorn University", "ENA first public:2017 10 02|ENA last update:2016 10 19|External Id:SAMEA4518350|INSDC center alias:School of Natural Science  Technology and Environmental Studies Sodertorn University|INSDC center name:School of Natural Science  Technology and Environmental Studies Sodertorn University|INSDC first public:2017 10 02T17:05:16Z|INSDC last update:2016 10 19T13:53:59Z|INSDC status:public|Submitter Id:E MTAB 5173:IonXpressRNA 007 up 058 7|age:200|broker name:ArrayExpress|common name:zebrafish|organism part:brain|sample name:E MTAB 5173:IonXpressRNA 007 up 058 7|sex:male", null, null, null, null, null, null, null, null, "Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17\u03b1 ethinylestradiol", "E MTAB 5173:IonXpressRNA 007 up 058 7 s", "IonXpressRNA 007 up 058 7 s", "RNA seq of the zebrafish danio rerio brain post developmental exposure to 17\u03b1 ethinylestradiol", "RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich  Germany EE2 exposure. Fish exposed to 0  2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA", "Experimental Factor: male:sex|Experimental Factor: n1:compound", "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM", "SINGLE", "ION_TORRENT", "Ion Torrent Proton", null, "ERP018188", "Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17\u00ce\u00b1 ethinylestradiol", "ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2018 11 16", "IonXpressRNA_007_up_058_7.fastq.gz", "fastq", 2307842164.0, 28984666.0, "E MTAB 5173:IonXpressRNA 007 up 058 7", "0:79.62", "A:634041732;C:548565089;G:545418985;T:579816358;N:0", 79, null, null, null, 634041732, 548565089, 545418985, 579816358, 0, "ERX1767855", "ERS1417529", "ERA739176", "School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive", "School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive", 1, 0.67542, null, 0.2817, null, 0.74168, null, 0.47224, null, 37, null, "B", null, "usable mapping rate", "ion_torrent", "ion_torrent", "full_length", "random_priming", "ribozero", "bulk", "unknown", "unknown", null, "Sweden", "2016-10-19", "Pharyngula", "Embryo", "Brain", "Nervous System"], [5801, "ERR1698346", "ERX1767854", "ERS1417528", "ERP018188", "PRJEB16335", "RNA seq of the zebrafish danio rerio brain post developmental exposure to 17a ethinylestradiol", "E-MTAB-5173", "Transcriptome Analysis", "17a ethinylestradiol EE2 is a synthetic estrogen commonly used as an active substance in oral contraceptives.  It is frequently found in waste water effluent and raise concern due to its persistent nature. EE2 binds to estrogen receptors with similar affinity to oestradiol and acts as one of the most potent hormone mimics found in the environment.  Estrogen is involved in many aspects of the development of the neuroendocrine system influencing both brain structure and behavior. We and others have reported a significant effect on non reproductive behaviors in adult fish and in recent studies we found that developmental exposure to EE2 resulted in an anxiogenic phenotype as adults even post a long remediation period.  In this study we aim to study possible mechanisms behind the behavior alterations of zebrafish developmentally exposed to EE2 by sequencing the whole brain transcriptome. Zebrafish embryos were exposed to 0  2.14 and 7.34 ng/L EE2 from 1 day to 80 dpf post the exposure period a remediation period of 120 days followed before the fish were sampled. 3 male brains from the control group 0 ng/L and the 2.14 ng/L group were sampled and 3 female brains from the control group 0 ng/L and 7.34 ng/L were sampled.", "ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2016 10 19|ArrayExpress:E MTAB 5173", null, "Protocols: RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich  Germany EE2 exposure. Fish exposed to 0  2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA", "IonXpressRNA 006 up 058 6", "SAMEA4518349", "School of Natural Science, Technology and Environmental Studies Sodertorn University", "ENA first public:2017 10 02|ENA last update:2016 10 19|External Id:SAMEA4518349|INSDC center alias:School of Natural Science  Technology and Environmental Studies Sodertorn University|INSDC center name:School of Natural Science  Technology and Environmental Studies Sodertorn University|INSDC first public:2017 10 02T17:05:16Z|INSDC last update:2016 10 19T13:53:59Z|INSDC status:public|Submitter Id:E MTAB 5173:IonXpressRNA 006 up 058 6|age:200|broker name:ArrayExpress|common name:zebrafish|organism part:brain|sample name:E MTAB 5173:IonXpressRNA 006 up 058 6|sex:female", null, null, null, null, null, null, null, null, "Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17\u03b1 ethinylestradiol", "E MTAB 5173:IonXpressRNA 006 up 058 6 s", "IonXpressRNA 006 up 058 6 s", "RNA seq of the zebrafish danio rerio brain post developmental exposure to 17\u03b1 ethinylestradiol", "RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich  Germany EE2 exposure. Fish exposed to 0  2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA", "Experimental Factor: female:sex|Experimental Factor: 17 ethinylestradiol:compound|Experimental Factor: 7.34:dose", "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM", "SINGLE", "ION_TORRENT", "Ion Torrent Proton", null, "ERP018188", "Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17\u00ce\u00b1 ethinylestradiol", "ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2018 11 16", "IonXpressRNA_006_up_058_6.fastq.gz", "fastq", 2239484857.0, 27021234.0, "E MTAB 5173:IonXpressRNA 006 up 058 6", "0:82.88", "A:637326624;C:506830056;G:503508207;T:591819970;N:0", 82, null, null, null, 637326624, 506830056, 503508207, 591819970, 0, "ERX1767854", "ERS1417528", "ERA739176", "School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive", "School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive", 1, 0.66441, null, 0.31904, null, 0.74905, null, 0.47154, null, 122, null, "B", null, "usable mapping rate", "ion_torrent", "ion_torrent", "full_length", "random_priming", "ribozero", "bulk", "unknown", "unknown", null, "Sweden", "2016-10-19", "Pharyngula", "Embryo", "Brain", "Nervous System"], [5802, "ERR1698345", "ERX1767853", "ERS1417527", "ERP018188", "PRJEB16335", "RNA seq of the zebrafish danio rerio brain post developmental exposure to 17a ethinylestradiol", "E-MTAB-5173", "Transcriptome Analysis", "17a ethinylestradiol EE2 is a synthetic estrogen commonly used as an active substance in oral contraceptives.  It is frequently found in waste water effluent and raise concern due to its persistent nature. EE2 binds to estrogen receptors with similar affinity to oestradiol and acts as one of the most potent hormone mimics found in the environment.  Estrogen is involved in many aspects of the development of the neuroendocrine system influencing both brain structure and behavior. We and others have reported a significant effect on non reproductive behaviors in adult fish and in recent studies we found that developmental exposure to EE2 resulted in an anxiogenic phenotype as adults even post a long remediation period.  In this study we aim to study possible mechanisms behind the behavior alterations of zebrafish developmentally exposed to EE2 by sequencing the whole brain transcriptome. Zebrafish embryos were exposed to 0  2.14 and 7.34 ng/L EE2 from 1 day to 80 dpf post the exposure period a remediation period of 120 days followed before the fish were sampled. 3 male brains from the control group 0 ng/L and the 2.14 ng/L group were sampled and 3 female brains from the control group 0 ng/L and 7.34 ng/L were sampled.", "ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2016 10 19|ArrayExpress:E MTAB 5173", null, "Protocols: RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich  Germany EE2 exposure. Fish exposed to 0  2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA", "IonXpressRNA 005 up 058 5", "SAMEA4518348", "School of Natural Science, Technology and Environmental Studies Sodertorn University", "ENA first public:2017 10 02|ENA last update:2016 10 19|External Id:SAMEA4518348|INSDC center alias:School of Natural Science  Technology and Environmental Studies Sodertorn University|INSDC center name:School of Natural Science  Technology and Environmental Studies Sodertorn University|INSDC first public:2017 10 02T17:05:16Z|INSDC last update:2016 10 19T13:53:59Z|INSDC status:public|Submitter Id:E MTAB 5173:IonXpressRNA 005 up 058 5|age:200|broker name:ArrayExpress|common name:zebrafish|organism part:brain|sample name:E MTAB 5173:IonXpressRNA 005 up 058 5|sex:female", null, null, null, null, null, null, null, null, "Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17\u03b1 ethinylestradiol", "E MTAB 5173:IonXpressRNA 005 up 058 5 s", "IonXpressRNA 005 up 058 5 s", "RNA seq of the zebrafish danio rerio brain post developmental exposure to 17\u03b1 ethinylestradiol", "RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich  Germany EE2 exposure. Fish exposed to 0  2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA", "Experimental Factor: female:sex|Experimental Factor: 17 ethinylestradiol:compound|Experimental Factor: 7.34:dose", "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM", "SINGLE", "ION_TORRENT", "Ion Torrent Proton", null, "ERP018188", "Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17\u00ce\u00b1 ethinylestradiol", "ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2018 11 16", "IonXpressRNA_005_up_058_5.fastq.gz", "fastq", 2920608487.0, 39573075.0, "E MTAB 5173:IonXpressRNA 005 up 058 5", "0:73.80", "A:814851327;C:678116994;G:691911192;T:735728974;N:0", 73, null, null, null, 814851327, 678116994, 691911192, 735728974, 0, "ERX1767853", "ERS1417527", "ERA739176", "School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive", "School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive", 1, 0.60005, null, 0.25542, null, 0.76481, null, 0.4886, null, 12, null, "B", null, "usable mapping rate", "ion_torrent", "ion_torrent", "full_length", "random_priming", "ribozero", "bulk", "unknown", "unknown", null, "Sweden", "2016-10-19", "Pharyngula", "Embryo", "Brain", "Nervous System"], [5803, "ERR1698344", "ERX1767852", "ERS1417526", "ERP018188", "PRJEB16335", "RNA seq of the zebrafish danio rerio brain post developmental exposure to 17a ethinylestradiol", "E-MTAB-5173", "Transcriptome Analysis", "17a ethinylestradiol EE2 is a synthetic estrogen commonly used as an active substance in oral contraceptives.  It is frequently found in waste water effluent and raise concern due to its persistent nature. EE2 binds to estrogen receptors with similar affinity to oestradiol and acts as one of the most potent hormone mimics found in the environment.  Estrogen is involved in many aspects of the development of the neuroendocrine system influencing both brain structure and behavior. We and others have reported a significant effect on non reproductive behaviors in adult fish and in recent studies we found that developmental exposure to EE2 resulted in an anxiogenic phenotype as adults even post a long remediation period.  In this study we aim to study possible mechanisms behind the behavior alterations of zebrafish developmentally exposed to EE2 by sequencing the whole brain transcriptome. Zebrafish embryos were exposed to 0  2.14 and 7.34 ng/L EE2 from 1 day to 80 dpf post the exposure period a remediation period of 120 days followed before the fish were sampled. 3 male brains from the control group 0 ng/L and the 2.14 ng/L group were sampled and 3 female brains from the control group 0 ng/L and 7.34 ng/L were sampled.", "ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2016 10 19|ArrayExpress:E MTAB 5173", null, "Protocols: RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich  Germany EE2 exposure. Fish exposed to 0  2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA", "IonXpressRNA 004 up 058 4", "SAMEA4518347", "School of Natural Science, Technology and Environmental Studies Sodertorn University", "ENA first public:2017 10 02|ENA last update:2016 10 19|External Id:SAMEA4518347|INSDC center alias:School of Natural Science  Technology and Environmental Studies Sodertorn University|INSDC center name:School of Natural Science  Technology and Environmental Studies Sodertorn University|INSDC first public:2017 10 02T17:05:16Z|INSDC last update:2016 10 19T13:53:59Z|INSDC status:public|Submitter Id:E MTAB 5173:IonXpressRNA 004 up 058 4|age:200|broker name:ArrayExpress|common name:zebrafish|organism part:brain|sample name:E MTAB 5173:IonXpressRNA 004 up 058 4|sex:female", null, null, null, null, null, null, null, null, "Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17\u03b1 ethinylestradiol", "E MTAB 5173:IonXpressRNA 004 up 058 4 s", "IonXpressRNA 004 up 058 4 s", "RNA seq of the zebrafish danio rerio brain post developmental exposure to 17\u03b1 ethinylestradiol", "RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich  Germany EE2 exposure. Fish exposed to 0  2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA", "Experimental Factor: female:sex|Experimental Factor: 17 ethinylestradiol:compound|Experimental Factor: 7.34:dose", "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM", "SINGLE", "ION_TORRENT", "Ion Torrent Proton", null, "ERP018188", "Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17\u00ce\u00b1 ethinylestradiol", "ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2018 11 16", "IonXpressRNA_004_up_058_4.fastq.gz", "fastq", 2989727396.0, 37953454.0, "E MTAB 5173:IonXpressRNA 004 up 058 4", "0:78.77", "A:802963810;C:722434772;G:742850530;T:721478284;N:0", 78, null, null, null, 802963810, 722434772, 742850530, 721478284, 0, "ERX1767852", "ERS1417526", "ERA739176", "School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive", "School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive", 1, 0.76402, null, 0.32503, null, 0.74241, null, 0.4815, null, 128, null, "B", null, "usable mapping rate", "ion_torrent", "ion_torrent", "full_length", "random_priming", "ribozero", "bulk", "unknown", "unknown", null, "Sweden", "2016-10-19", "Pharyngula", "Embryo", "Brain", "Nervous System"], [5804, "ERR1698343", "ERX1767851", "ERS1417525", "ERP018188", "PRJEB16335", "RNA seq of the zebrafish danio rerio brain post developmental exposure to 17a ethinylestradiol", "E-MTAB-5173", "Transcriptome Analysis", "17a ethinylestradiol EE2 is a synthetic estrogen commonly used as an active substance in oral contraceptives.  It is frequently found in waste water effluent and raise concern due to its persistent nature. EE2 binds to estrogen receptors with similar affinity to oestradiol and acts as one of the most potent hormone mimics found in the environment.  Estrogen is involved in many aspects of the development of the neuroendocrine system influencing both brain structure and behavior. We and others have reported a significant effect on non reproductive behaviors in adult fish and in recent studies we found that developmental exposure to EE2 resulted in an anxiogenic phenotype as adults even post a long remediation period.  In this study we aim to study possible mechanisms behind the behavior alterations of zebrafish developmentally exposed to EE2 by sequencing the whole brain transcriptome. Zebrafish embryos were exposed to 0  2.14 and 7.34 ng/L EE2 from 1 day to 80 dpf post the exposure period a remediation period of 120 days followed before the fish were sampled. 3 male brains from the control group 0 ng/L and the 2.14 ng/L group were sampled and 3 female brains from the control group 0 ng/L and 7.34 ng/L were sampled.", "ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2016 10 19|ArrayExpress:E MTAB 5173", null, "Protocols: RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich  Germany EE2 exposure. Fish exposed to 0  2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA", "IonXpressRNA 003 up 058 3", "SAMEA4518346", "School of Natural Science, Technology and Environmental Studies Sodertorn University", "ENA first public:2017 10 02|ENA last update:2016 10 19|External Id:SAMEA4518346|INSDC center alias:School of Natural Science  Technology and Environmental Studies Sodertorn University|INSDC center name:School of Natural Science  Technology and Environmental Studies Sodertorn University|INSDC first public:2017 10 02T17:05:16Z|INSDC last update:2016 10 19T13:53:59Z|INSDC status:public|Submitter Id:E MTAB 5173:IonXpressRNA 003 up 058 3|age:200|broker name:ArrayExpress|common name:zebrafish|organism part:brain|sample name:E MTAB 5173:IonXpressRNA 003 up 058 3|sex:female", null, null, null, null, null, null, null, null, "Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17\u03b1 ethinylestradiol", "E MTAB 5173:IonXpressRNA 003 up 058 3 s", "IonXpressRNA 003 up 058 3 s", "RNA seq of the zebrafish danio rerio brain post developmental exposure to 17\u03b1 ethinylestradiol", "RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich  Germany EE2 exposure. Fish exposed to 0  2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA", "Experimental Factor: female:sex|Experimental Factor: n1:compound", "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM", "SINGLE", "ION_TORRENT", "Ion Torrent Proton", null, "ERP018188", "Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17\u00ce\u00b1 ethinylestradiol", "ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2018 11 16", "IonXpressRNA_003_up_058_3.fastq.gz", "fastq", 4068383115.0, 41996847.0, "E MTAB 5173:IonXpressRNA 003 up 058 3", "0:96.87", "A:1133167706;C:944306379;G:948626944;T:1042282086;N:0", 96, null, null, null, 1133167706, 944306379, 948626944, 1042282086, 0, "ERX1767851", "ERS1417525", "ERA739176", "School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive", "School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive", 1, 0.54804, null, 0.22334, null, 0.76353, null, 0.49163, null, 93, null, "B", null, "usable mapping rate", "ion_torrent", "ion_torrent", "full_length", "random_priming", "ribozero", "bulk", "unknown", "unknown", null, "Sweden", "2016-10-19", "Pharyngula", "Embryo", "Brain", "Nervous System"], [5805, "ERR1698342", "ERX1767850", "ERS1417524", "ERP018188", "PRJEB16335", "RNA seq of the zebrafish danio rerio brain post developmental exposure to 17a ethinylestradiol", "E-MTAB-5173", "Transcriptome Analysis", "17a ethinylestradiol EE2 is a synthetic estrogen commonly used as an active substance in oral contraceptives.  It is frequently found in waste water effluent and raise concern due to its persistent nature. EE2 binds to estrogen receptors with similar affinity to oestradiol and acts as one of the most potent hormone mimics found in the environment.  Estrogen is involved in many aspects of the development of the neuroendocrine system influencing both brain structure and behavior. We and others have reported a significant effect on non reproductive behaviors in adult fish and in recent studies we found that developmental exposure to EE2 resulted in an anxiogenic phenotype as adults even post a long remediation period.  In this study we aim to study possible mechanisms behind the behavior alterations of zebrafish developmentally exposed to EE2 by sequencing the whole brain transcriptome. Zebrafish embryos were exposed to 0  2.14 and 7.34 ng/L EE2 from 1 day to 80 dpf post the exposure period a remediation period of 120 days followed before the fish were sampled. 3 male brains from the control group 0 ng/L and the 2.14 ng/L group were sampled and 3 female brains from the control group 0 ng/L and 7.34 ng/L were sampled.", "ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2016 10 19|ArrayExpress:E MTAB 5173", null, "Protocols: RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich  Germany EE2 exposure. Fish exposed to 0  2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA", "IonXpressRNA 002 up 058 2", "SAMEA4518345", "School of Natural Science, Technology and Environmental Studies Sodertorn University", "ENA first public:2017 10 02|ENA last update:2016 10 19|External Id:SAMEA4518345|INSDC center alias:School of Natural Science  Technology and Environmental Studies Sodertorn University|INSDC center name:School of Natural Science  Technology and Environmental Studies Sodertorn University|INSDC first public:2017 10 02T17:05:16Z|INSDC last update:2016 10 19T13:53:59Z|INSDC status:public|Submitter Id:E MTAB 5173:IonXpressRNA 002 up 058 2|age:200|broker name:ArrayExpress|common name:zebrafish|organism part:brain|sample name:E MTAB 5173:IonXpressRNA 002 up 058 2|sex:female", null, null, null, null, null, null, null, null, "Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17\u03b1 ethinylestradiol", "E MTAB 5173:IonXpressRNA 002 up 058 2 s", "IonXpressRNA 002 up 058 2 s", "RNA seq of the zebrafish danio rerio brain post developmental exposure to 17\u03b1 ethinylestradiol", "RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich  Germany EE2 exposure. Fish exposed to 0  2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA", "Experimental Factor: female:sex|Experimental Factor: n1:compound", "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM", "SINGLE", "ION_TORRENT", "Ion Torrent Proton", null, "ERP018188", "Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17\u00ce\u00b1 ethinylestradiol", "ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2018 11 16", "IonXpressRNA_002_up_058_2.fastq.gz", "fastq", 2679963893.0, 33552723.0, "E MTAB 5173:IonXpressRNA 002 up 058 2", "0:79.87", "A:709092431;C:635329399;G:670478210;T:665063853;N:0", 79, null, null, null, 709092431, 635329399, 670478210, 665063853, 0, "ERX1767850", "ERS1417524", "ERA739176", "School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive", "School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive", 1, 0.43283, null, 0.18649, null, 0.81178, null, 0.52885, null, 116, null, "B", null, "usable mapping rate", "ion_torrent", "ion_torrent", "full_length", "random_priming", "ribozero", "bulk", "unknown", "unknown", null, "Sweden", "2016-10-19", "Pharyngula", "Embryo", "Brain", "Nervous System"], [5806, "ERR1698341", "ERX1767849", "ERS1417523", "ERP018188", "PRJEB16335", "RNA seq of the zebrafish danio rerio brain post developmental exposure to 17a ethinylestradiol", "E-MTAB-5173", "Transcriptome Analysis", "17a ethinylestradiol EE2 is a synthetic estrogen commonly used as an active substance in oral contraceptives.  It is frequently found in waste water effluent and raise concern due to its persistent nature. EE2 binds to estrogen receptors with similar affinity to oestradiol and acts as one of the most potent hormone mimics found in the environment.  Estrogen is involved in many aspects of the development of the neuroendocrine system influencing both brain structure and behavior. We and others have reported a significant effect on non reproductive behaviors in adult fish and in recent studies we found that developmental exposure to EE2 resulted in an anxiogenic phenotype as adults even post a long remediation period.  In this study we aim to study possible mechanisms behind the behavior alterations of zebrafish developmentally exposed to EE2 by sequencing the whole brain transcriptome. Zebrafish embryos were exposed to 0  2.14 and 7.34 ng/L EE2 from 1 day to 80 dpf post the exposure period a remediation period of 120 days followed before the fish were sampled. 3 male brains from the control group 0 ng/L and the 2.14 ng/L group were sampled and 3 female brains from the control group 0 ng/L and 7.34 ng/L were sampled.", "ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2016 10 19|ArrayExpress:E MTAB 5173", null, "Protocols: RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich  Germany EE2 exposure. Fish exposed to 0  2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA", "IonXpressRNA 001 up 058 1", "SAMEA4518344", "School of Natural Science, Technology and Environmental Studies Sodertorn University", "ENA first public:2017 10 02|ENA last update:2016 10 19|External Id:SAMEA4518344|INSDC center alias:School of Natural Science  Technology and Environmental Studies Sodertorn University|INSDC center name:School of Natural Science  Technology and Environmental Studies Sodertorn University|INSDC first public:2017 10 02T17:05:16Z|INSDC last update:2016 10 19T13:53:59Z|INSDC status:public|Submitter Id:E MTAB 5173:IonXpressRNA 001 up 058 1|age:200|broker name:ArrayExpress|common name:zebrafish|organism part:brain|sample name:E MTAB 5173:IonXpressRNA 001 up 058 1|sex:female", null, null, null, null, null, null, null, null, "Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17\u03b1 ethinylestradiol", "E MTAB 5173:IonXpressRNA 001 up 058 1 s", "IonXpressRNA 001 up 058 1 s", "RNA seq of the zebrafish danio rerio brain post developmental exposure to 17\u03b1 ethinylestradiol", "RNA extraction Whole brains homogenized in TriReagent according to the manufacturer 0.8 ml/sample Sigma Aldrich  Germany EE2 exposure. Fish exposed to 0  2.14 or 7.34 ng/L EE2 for 80 days starting 1 dpf Ion Whole transcriptome analysis kit on RiboZero eukaryote depleted total RNA", "Experimental Factor: female:sex|Experimental Factor: n1:compound", "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM", "SINGLE", "ION_TORRENT", "Ion Torrent Proton", null, "ERP018188", "Ion Torrent Proton sequencing; RNA seq of the zebrafish danio rerio brain post developmental exposure to 17\u00ce\u00b1 ethinylestradiol", "ENA FIRST PUBLIC:2017 10 02|ENA LAST UPDATE:2018 11 16", "IonXpressRNA_001_up_058_1.fastq.gz", "fastq", 3358902499.0, 39857024.0, "E MTAB 5173:IonXpressRNA 001 up 058 1", "0:84.27", "A:933405205;C:784111716;G:780404241;T:860981337;N:0", 84, null, null, null, 933405205, 784111716, 780404241, 860981337, 0, "ERX1767849", "ERS1417523", "ERA739176", "School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive", "School of Natural Science, Technology and Environmental Studies Sodertorn University|European Nucleotide Archive", 1, 0.71789, null, 0.33159, null, 0.76619, null, 0.514, null, 56, null, "B", null, "usable mapping rate", "ion_torrent", "ion_torrent", "full_length", "random_priming", "ribozero", "bulk", "unknown", "unknown", null, "Sweden", "2016-10-19", "Pharyngula", "Embryo", "Brain", "Nervous System"], [10407, "ERR9077202", "ERX8620336", "ERS10857224", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aWT5S8", "SAMEA13254181", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254181|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT5S8|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT5S8|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aWT5S8 s", "Sv2aWT5S8 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a+/+", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aWT5S8L001-1.fastq.gz", "fastq", 652046291.0, 8633105.0, "E MTAB 11505:sv2aWT5S8L001", "0:75.53 1:0", "A:159570243;C:156420495;G:154853315;T:181073030;N:129208", 75, 0, null, null, 159570243, 156420495, 154853315, 181073030, 129208, "ERX8620336", "ERS10857224", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.95245, null, 0.1148, null, 0.65756, null, 0.47011, null, 75, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10408, "ERR9077203", "ERX8620336", "ERS10857224", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aWT5S8", "SAMEA13254181", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254181|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT5S8|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT5S8|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aWT5S8 s", "Sv2aWT5S8 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a+/+", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aWT5S8L002-1.fastq.gz", "fastq", 653840525.0, 8656886.0, "E MTAB 11505:sv2aWT5S8L002", "0:75.53 1:0", "A:159902458;C:156826842;G:155509070;T:181475104;N:127051", 75, 0, null, null, 159902458, 156826842, 155509070, 181475104, 127051, "ERX8620336", "ERS10857224", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.95226, null, 0.1156, null, 0.65792, null, 0.4667, null, 76, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10409, "ERR9077204", "ERX8620336", "ERS10857224", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aWT5S8", "SAMEA13254181", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254181|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT5S8|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT5S8|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aWT5S8 s", "Sv2aWT5S8 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  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Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aWT5S8", "SAMEA13254181", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254181|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT5S8|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT5S8|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aWT5S8 s", "Sv2aWT5S8 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  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Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aWT4S7", "SAMEA13254180", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254180|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT4S7|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT4S7|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aWT4S7 s", "Sv2aWT4S7 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a+/+", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aWT4S7L001-1.fastq.gz", "fastq", 683751258.0, 9051795.0, "E MTAB 11505:sv2aWT4S7L001", "0:75.54 1:0", "A:162889923;C:167127644;G:164779384;T:188822907;N:131400", 75, 0, null, null, 162889923, 167127644, 164779384, 188822907, 131400, "ERX8620335", "ERS10857223", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.95739, null, 0.0959, null, 0.6606, null, 0.46826, null, 75, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10412, "ERR9077199", "ERX8620335", "ERS10857223", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aWT4S7", "SAMEA13254180", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254180|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT4S7|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT4S7|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aWT4S7 s", "Sv2aWT4S7 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  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Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aWT4S7", "SAMEA13254180", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254180|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT4S7|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT4S7|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aWT4S7 s", "Sv2aWT4S7 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a+/+", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aWT4S7L003-1.fastq.gz", "fastq", 692320213.0, 9165132.0, "E MTAB 11505:sv2aWT4S7L003", "0:75.54 1:0", "A:164772792;C:169329920;G:166952052;T:191164422;N:101027", 75, 0, null, null, 164772792, 169329920, 166952052, 191164422, 101027, "ERX8620335", "ERS10857223", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.95786, null, 0.09701, null, 0.66131, null, 0.47564, null, 76, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10414, "ERR9077201", "ERX8620335", "ERS10857223", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aWT4S7", "SAMEA13254180", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254180|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT4S7|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT4S7|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aWT4S7 s", "Sv2aWT4S7 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a+/+", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aWT4S7L004-1.fastq.gz", "fastq", 697047881.0, 9227702.0, "E MTAB 11505:sv2aWT4S7L004", "0:75.54 1:0", "A:165881728;C:170388393;G:168105900;T:192572068;N:99792", 75, 0, null, null, 165881728, 170388393, 168105900, 192572068, 99792, "ERX8620335", "ERS10857223", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.9584, null, 0.09565, null, 0.66115, null, 0.46967, null, 76, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10415, "ERR9077194", "ERX8620334", "ERS10857222", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aWT3S2", "SAMEA13254179", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254179|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT3S2|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT3S2|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aWT3S2 s", "Sv2aWT3S2 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a+/+", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aWT3S2L001-1.fastq.gz", "fastq", 723508205.0, 9579193.0, "E MTAB 11505:sv2aWT3S2L001", "0:75.53 1:0", "A:177832489;C:174193135;G:169978598;T:201360184;N:143799", 75, 0, null, null, 177832489, 174193135, 169978598, 201360184, 143799, "ERX8620334", "ERS10857222", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.95218, null, 0.11732, null, 0.65916, null, 0.47955, null, 71, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10416, "ERR9077195", "ERX8620334", "ERS10857222", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aWT3S2", "SAMEA13254179", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254179|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT3S2|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT3S2|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aWT3S2 s", "Sv2aWT3S2 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a+/+", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aWT3S2L002-1.fastq.gz", "fastq", 726190024.0, 9614674.0, "E MTAB 11505:sv2aWT3S2L002", "0:75.53 1:0", "A:178408224;C:174776263;G:170820745;T:202047224;N:137568", 75, 0, null, null, 178408224, 174776263, 170820745, 202047224, 137568, "ERX8620334", "ERS10857222", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.95093, null, 0.11567, null, 0.65865, null, 0.46877, null, 75, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10417, "ERR9077196", "ERX8620334", "ERS10857222", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aWT3S2", "SAMEA13254179", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254179|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT3S2|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT3S2|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aWT3S2 s", "Sv2aWT3S2 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a+/+", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aWT3S2L003-1.fastq.gz", "fastq", 735499955.0, 9737845.0, "E MTAB 11505:sv2aWT3S2L003", "0:75.53 1:0", "A:180659962;C:177141362;G:172905323;T:204678308;N:115000", 75, 0, null, null, 180659962, 177141362, 172905323, 204678308, 115000, "ERX8620334", "ERS10857222", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.95233, null, 0.11614, null, 0.65898, null, 0.47114, null, 76, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10418, "ERR9077197", "ERX8620334", "ERS10857222", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aWT3S2", "SAMEA13254179", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254179|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT3S2|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT3S2|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aWT3S2 s", "Sv2aWT3S2 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a+/+", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aWT3S2L004-1.fastq.gz", "fastq", 745285512.0, 9867337.0, "E MTAB 11505:sv2aWT3S2L004", "0:75.53 1:0", "A:183067487;C:179408382;G:175250814;T:207445912;N:112917", 75, 0, null, null, 183067487, 179408382, 175250814, 207445912, 112917, "ERX8620334", "ERS10857222", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.95245, null, 0.11655, null, 0.65857, null, 0.47087, null, 76, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10419, "ERR9077190", "ERX8620333", "ERS10857221", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aWT1S1", "SAMEA13254178", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254178|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT1S1|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT1S1|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aWT1S1 s", "Sv2aWT1S1 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a+/+", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aWT1S1L001-1.fastq.gz", "fastq", 647414748.0, 8571883.0, "E MTAB 11505:sv2aWT1S1L001", "0:75.53 1:0", "A:154593859;C:160498057;G:153809612;T:178389112;N:124108", 75, 0, null, null, 154593859, 160498057, 153809612, 178389112, 124108, "ERX8620333", "ERS10857221", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.95955, null, 0.08686, null, 0.65955, null, 0.47406, null, 76, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10420, "ERR9077191", "ERX8620333", "ERS10857221", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aWT1S1", "SAMEA13254178", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254178|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT1S1|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT1S1|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aWT1S1 s", "Sv2aWT1S1 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a+/+", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aWT1S1L002-1.fastq.gz", "fastq", 646657758.0, 8561861.0, "E MTAB 11505:sv2aWT1S1L002", "0:75.53 1:0", "A:154348044;C:160294625;G:153804048;T:178092212;N:118829", 75, 0, null, null, 154348044, 160294625, 153804048, 178092212, 118829, "ERX8620333", "ERS10857221", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.95942, null, 0.08632, null, 0.65991, null, 0.47539, null, 76, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10421, "ERR9077192", "ERX8620333", "ERS10857221", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aWT1S1", "SAMEA13254178", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254178|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT1S1|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT1S1|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aWT1S1 s", "Sv2aWT1S1 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  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Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aWT1S1", "SAMEA13254178", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254178|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aWT1S1|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/+|individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aWT1S1|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aWT1S1 s", "Sv2aWT1S1 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  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Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aHO6S12", "SAMEA13254177", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254177|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO6S12|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO6S12|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aHO6S12 s", "Sv2aHO6S12 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  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Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aHO6S12", "SAMEA13254177", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254177|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO6S12|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO6S12|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aHO6S12 s", "Sv2aHO6S12 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a / ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aHO6S12L002-1.fastq.gz", "fastq", 672995667.0, 8911161.0, "E MTAB 11505:sv2aHO6S12L002", "0:75.52 1:0", "A:165990319;C:160039398;G:159722501;T:187108781;N:134668", 75, 0, null, null, 165990319, 160039398, 159722501, 187108781, 134668, "ERX8620332", "ERS10857220", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.94837, null, 0.13029, null, 0.65744, null, 0.47959, null, 76, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10425, "ERR9077188", "ERX8620332", "ERS10857220", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aHO6S12", "SAMEA13254177", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254177|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO6S12|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO6S12|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aHO6S12 s", "Sv2aHO6S12 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a / ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aHO6S12L003-1.fastq.gz", "fastq", 683276767.0, 9047147.0, "E MTAB 11505:sv2aHO6S12L003", "0:75.52 1:0", "A:168501690;C:162583852;G:162063190;T:190018001;N:110034", 75, 0, null, null, 168501690, 162583852, 162063190, 190018001, 110034, "ERX8620332", "ERS10857220", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.94983, null, 0.13248, null, 0.6565, null, 0.47977, null, 76, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10426, "ERR9077189", "ERX8620332", "ERS10857220", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aHO6S12", "SAMEA13254177", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254177|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO6S12|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO6S12|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aHO6S12 s", "Sv2aHO6S12 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a / ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aHO6S12L004-1.fastq.gz", "fastq", 686469910.0, 9089431.0, "E MTAB 11505:sv2aHO6S12L004", "0:75.52 1:0", "A:169287189;C:163276361;G:162844724;T:190954218;N:107418", 75, 0, null, null, 169287189, 163276361, 162844724, 190954218, 107418, "ERX8620332", "ERS10857220", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.95061, null, 0.13029, null, 0.65389, null, 0.48044, null, 76, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10427, "ERR9077182", "ERX8620331", "ERS10857219", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aHO4S10", "SAMEA13254176", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254176|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO4S10|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO4S10|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aHO4S10 s", "Sv2aHO4S10 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a / ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aHO4S10L001-1.fastq.gz", "fastq", 713858966.0, 9452474.0, "E MTAB 11505:sv2aHO4S10L001", "0:75.52 1:0", "A:177913695;C:169425317;G:167540012;T:198835532;N:144410", 75, 0, null, null, 177913695, 169425317, 167540012, 198835532, 144410, "ERX8620331", "ERS10857219", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.94848, null, 0.12981, null, 0.6636, null, 0.47772, null, 76, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10428, "ERR9077183", "ERX8620331", "ERS10857219", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aHO4S10", "SAMEA13254176", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254176|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO4S10|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO4S10|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aHO4S10 s", "Sv2aHO4S10 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a / ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aHO4S10L002-1.fastq.gz", "fastq", 717619593.0, 9502301.0, "E MTAB 11505:sv2aHO4S10L002", "0:75.52 1:0", "A:178830542;C:170284720;G:168594514;T:199773382;N:136435", 75, 0, null, null, 178830542, 170284720, 168594514, 199773382, 136435, "ERX8620331", "ERS10857219", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.94812, null, 0.12868, null, 0.6645, null, 0.47281, null, 75, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10429, "ERR9077184", "ERX8620331", "ERS10857219", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aHO4S10", "SAMEA13254176", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254176|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO4S10|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO4S10|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aHO4S10 s", "Sv2aHO4S10 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a / ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aHO4S10L003-1.fastq.gz", "fastq", 727263626.0, 9629858.0, "E MTAB 11505:sv2aHO4S10L003", "0:75.52 1:0", "A:181201278;C:172619520;G:170780953;T:202550397;N:111478", 75, 0, null, null, 181201278, 172619520, 170780953, 202550397, 111478, "ERX8620331", "ERS10857219", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.94768, null, 0.12827, null, 0.66596, null, 0.47332, null, 76, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10430, "ERR9077185", "ERX8620331", "ERS10857219", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aHO4S10", "SAMEA13254176", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254176|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO4S10|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO4S10|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aHO4S10 s", "Sv2aHO4S10 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  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Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aHO3S6", "SAMEA13254175", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254175|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO3S6|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO3S6|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aHO3S6 s", "Sv2aHO3S6 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a / ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aHO3S6L001-1.fastq.gz", "fastq", 710756305.0, 9410862.0, "E MTAB 11505:sv2aHO3S6L001", "0:75.53 1:0", "A:175585613;C:170261120;G:166658917;T:198106388;N:144267", 75, 0, null, null, 175585613, 170261120, 166658917, 198106388, 144267, "ERX8620330", "ERS10857218", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.94827, null, 0.14002, null, 0.66785, null, 0.474, null, 75, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10432, "ERR9077179", "ERX8620330", "ERS10857218", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aHO3S6", "SAMEA13254175", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254175|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO3S6|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO3S6|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aHO3S6 s", "Sv2aHO3S6 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a / ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aHO3S6L002-1.fastq.gz", "fastq", 715330324.0, 9471415.0, "E MTAB 11505:sv2aHO3S6L002", "0:75.53 1:0", "A:176679775;C:171311031;G:167903237;T:199299483;N:136798", 75, 0, null, null, 176679775, 171311031, 167903237, 199299483, 136798, "ERX8620330", "ERS10857218", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.9469, null, 0.13915, null, 0.67004, null, 0.47654, null, 75, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10433, "ERR9077180", "ERX8620330", "ERS10857218", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aHO3S6", "SAMEA13254175", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254175|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO3S6|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO3S6|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aHO3S6 s", "Sv2aHO3S6 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a / ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aHO3S6L003-1.fastq.gz", "fastq", 722810562.0, 9570364.0, "E MTAB 11505:sv2aHO3S6L003", "0:75.53 1:0", "A:178442845;C:173192786;G:169595085;T:201465522;N:114324", 75, 0, null, null, 178442845, 173192786, 169595085, 201465522, 114324, "ERX8620330", "ERS10857218", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.94918, null, 0.13979, null, 0.66902, null, 0.47572, null, 75, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10434, "ERR9077181", "ERX8620330", "ERS10857218", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aHO3S6", "SAMEA13254175", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254175|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHO3S6|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a / |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHO3S6|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aHO3S6 s", "Sv2aHO3S6 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a / ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aHO3S6L004-1.fastq.gz", "fastq", 732990507.0, 9705054.0, "E MTAB 11505:sv2aHO3S6L004", "0:75.53 1:0", "A:180951130;C:175567778;G:172037542;T:204323488;N:110569", 75, 0, null, null, 180951130, 175567778, 172037542, 204323488, 110569, "ERX8620330", "ERS10857218", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.94812, null, 0.13893, null, 0.67085, null, 0.47502, null, 75, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10435, "ERR9077174", "ERX8620329", "ERS10857217", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aHET7S9", "SAMEA13254174", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254174|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET7S9|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET7S9|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aHET7S9 s", "Sv2aHET7S9 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  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Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aHET7S9", "SAMEA13254174", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254174|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET7S9|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET7S9|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aHET7S9 s", "Sv2aHET7S9 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a+/ ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aHET7S9L002-1.fastq.gz", "fastq", 773106152.0, 10236677.0, "E MTAB 11505:sv2aHET7S9L002", "0:75.52 1:0", "A:191812833;C:184676419;G:180997547;T:215473732;N:145621", 75, 0, null, null, 191812833, 184676419, 180997547, 215473732, 145621, "ERX8620329", "ERS10857217", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.95016, null, 0.12464, null, 0.65482, null, 0.47419, null, 76, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10437, "ERR9077176", "ERX8620329", "ERS10857217", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aHET7S9", "SAMEA13254174", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254174|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET7S9|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET7S9|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aHET7S9 s", "Sv2aHET7S9 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a+/ ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aHET7S9L003-1.fastq.gz", "fastq", 783061051.0, 10368445.0, "E MTAB 11505:sv2aHET7S9L003", "0:75.52 1:0", "A:194289152;C:187108364;G:183177036;T:218370500;N:115999", 75, 0, null, null, 194289152, 187108364, 183177036, 218370500, 115999, "ERX8620329", "ERS10857217", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.95172, null, 0.12488, null, 0.65445, null, 0.48157, null, 76, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10438, "ERR9077177", "ERX8620329", "ERS10857217", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aHET7S9", "SAMEA13254174", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254174|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET7S9|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET7S9|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aHET7S9 s", "Sv2aHET7S9 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a+/ ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aHET7S9L004-1.fastq.gz", "fastq", 790150025.0, 10462214.0, "E MTAB 11505:sv2aHET7S9L004", "0:75.52 1:0", "A:196056500;C:188746833;G:184927588;T:220305922;N:113182", 75, 0, null, null, 196056500, 188746833, 184927588, 220305922, 113182, "ERX8620329", "ERS10857217", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.95285, null, 0.12478, null, 0.65551, null, 0.4763, null, 76, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10439, "ERR9077170", "ERX8620328", "ERS10857216", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aHET4S5", "SAMEA13254173", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254173|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET4S5|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET4S5|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aHET4S5 s", "Sv2aHET4S5 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a+/ ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aHET4S5L001-1.fastq.gz", "fastq", 685650097.0, 9076481.0, "E MTAB 11505:sv2aHET4S5L001", "0:75.54 1:0", "A:161812875;C:167870520;G:166985310;T:188850530;N:130862", 75, 0, null, null, 161812875, 167870520, 166985310, 188850530, 130862, "ERX8620328", "ERS10857216", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.95733, null, 0.09974, null, 0.65691, null, 0.47392, null, 76, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10440, "ERR9077171", "ERX8620328", "ERS10857216", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aHET4S5", "SAMEA13254173", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254173|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET4S5|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET4S5|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aHET4S5 s", "Sv2aHET4S5 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a+/ ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aHET4S5L002-1.fastq.gz", "fastq", 684402241.0, 9059974.0, "E MTAB 11505:sv2aHET4S5L002", "0:75.54 1:0", "A:161493428;C:167520248;G:166882054;T:188383816;N:122695", 75, 0, null, null, 161493428, 167520248, 166882054, 188383816, 122695, "ERX8620328", "ERS10857216", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.95537, null, 0.09915, null, 0.65719, null, 0.48261, null, 76, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10441, "ERR9077172", "ERX8620328", "ERS10857216", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aHET4S5", "SAMEA13254173", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254173|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET4S5|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET4S5|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aHET4S5 s", "Sv2aHET4S5 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a+/ ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aHET4S5L003-1.fastq.gz", "fastq", 694099126.0, 9188245.0, "E MTAB 11505:sv2aHET4S5L003", "0:75.54 1:0", "A:163727378;C:169989210;G:169143224;T:191137588;N:101726", 75, 0, null, null, 163727378, 169989210, 169143224, 191137588, 101726, "ERX8620328", "ERS10857216", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.95629, null, 0.09861, null, 0.65705, null, 0.47187, null, 75, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10442, "ERR9077173", "ERX8620328", "ERS10857216", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aHET4S5", "SAMEA13254173", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254173|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET4S5|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET4S5|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aHET4S5 s", "Sv2aHET4S5 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a+/ ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aHET4S5L004-1.fastq.gz", "fastq", 698753836.0, 9249820.0, "E MTAB 11505:sv2aHET4S5L004", "0:75.54 1:0", "A:164809473;C:171057633;G:170349692;T:192441413;N:95625", 75, 0, null, null, 164809473, 171057633, 170349692, 192441413, 95625, "ERX8620328", "ERS10857216", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.95613, null, 0.09885, null, 0.65798, null, 0.47227, null, 76, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10443, "ERR9077166", "ERX8620327", "ERS10857215", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aHET3S4", "SAMEA13254172", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254172|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET3S4|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET3S4|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aHET3S4 s", "Sv2aHET3S4 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a+/ ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aHET3S4L001-1.fastq.gz", "fastq", 691298605.0, 9151512.0, "E MTAB 11505:sv2aHET3S4L001", "0:75.54 1:0", "A:164743919;C:170752099;G:164763621;T:190912044;N:126922", 75, 0, null, null, 164743919, 170752099, 164763621, 190912044, 126922, "ERX8620327", "ERS10857215", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.95684, null, 0.09604, null, 0.6603, null, 0.47285, null, 76, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10444, "ERR9077167", "ERX8620327", "ERS10857215", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aHET3S4", "SAMEA13254172", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254172|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET3S4|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET3S4|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aHET3S4 s", "Sv2aHET3S4 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a+/ ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aHET3S4L002-1.fastq.gz", "fastq", 694080975.0, 9188259.0, "E MTAB 11505:sv2aHET3S4L002", "0:75.54 1:0", "A:165336810;C:171397273;G:165632321;T:191591872;N:122699", 75, 0, null, null, 165336810, 171397273, 165632321, 191591872, 122699, "ERX8620327", "ERS10857215", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.95612, null, 0.09694, null, 0.65993, null, 0.46918, null, 76, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10445, "ERR9077168", "ERX8620327", "ERS10857215", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aHET3S4", "SAMEA13254172", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254172|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET3S4|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET3S4|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aHET3S4 s", "Sv2aHET3S4 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a+/ ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aHET3S4L003-1.fastq.gz", "fastq", 703689889.0, 9315387.0, "E MTAB 11505:sv2aHET3S4L003", "0:75.54 1:0", "A:167575230;C:173881977;G:167845574;T:194289403;N:97705", 75, 0, null, null, 167575230, 173881977, 167845574, 194289403, 97705, "ERX8620327", "ERS10857215", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.9577, null, 0.09608, null, 0.66042, null, 0.47022, null, 74, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10446, "ERR9077169", "ERX8620327", "ERS10857215", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aHET3S4", "SAMEA13254172", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254172|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET3S4|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET3S4|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aHET3S4 s", "Sv2aHET3S4 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a+/ ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aHET3S4L004-1.fastq.gz", "fastq", 712116538.0, 9426961.0, "E MTAB 11505:sv2aHET3S4L004", "0:75.54 1:0", "A:169598596;C:175885948;G:169866534;T:196669400;N:96060", 75, 0, null, null, 169598596, 175885948, 169866534, 196669400, 96060, "ERX8620327", "ERS10857215", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.95734, null, 0.09716, null, 0.66054, null, 0.47257, null, 75, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10447, "ERR9077162", "ERX8620326", "ERS10857214", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aHET2S3", "SAMEA13254171", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254171|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET2S3|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET2S3|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aHET2S3 s", "Sv2aHET2S3 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a+/ ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aHET2S3L001-1.fastq.gz", "fastq", 733440522.0, 9709422.0, "E MTAB 11505:sv2aHET2S3L001", "0:75.54 1:0", "A:173670000;C:181175353;G:175392793;T:203059256;N:143120", 75, 0, null, null, 173670000, 181175353, 175392793, 203059256, 143120, "ERX8620326", "ERS10857214", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.95885, null, 0.08758, null, 0.65393, null, 0.47249, null, 76, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10448, "ERR9077163", "ERX8620326", "ERS10857214", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aHET2S3", "SAMEA13254171", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254171|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET2S3|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET2S3|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aHET2S3 s", "Sv2aHET2S3 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a+/ ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aHET2S3L002-1.fastq.gz", "fastq", 732121929.0, 9691967.0, "E MTAB 11505:sv2aHET2S3L002", "0:75.54 1:0", "A:173332091;C:180805738;G:175271736;T:202574727;N:137637", 75, 0, null, null, 173332091, 180805738, 175271736, 202574727, 137637, "ERX8620326", "ERS10857214", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.95775, null, 0.08879, null, 0.6564, null, 0.4718, null, 76, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10449, "ERR9077164", "ERX8620326", "ERS10857214", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aHET2S3", "SAMEA13254171", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254171|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET2S3|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET2S3|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aHET2S3 s", "Sv2aHET2S3 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a+/ ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aHET2S3L003-1.fastq.gz", "fastq", 743488189.0, 9842286.0, "E MTAB 11505:sv2aHET2S3L003", "0:75.54 1:0", "A:175979238;C:183726319;G:177933958;T:205735955;N:112719", 75, 0, null, null, 175979238, 183726319, 177933958, 205735955, 112719, "ERX8620326", "ERS10857214", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.9593, null, 0.08716, null, 0.65437, null, 0.46976, null, 75, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [10450, "ERR9077165", "ERX8620326", "ERS10857214", "ERP135966", "PRJEB51339", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E-MTAB-11505", "Transcriptome Analysis", "Transcriptome analysis of the heads of 6 dpf sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae to provide insights into the neuropathological processes underlying the observed epileptic phenotype in sv2a /  zebrafish larvae.", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", null, "Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas. TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Sv2aHET2S3", "SAMEA13254171", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg", "ENA first public:2022 04 21|ENA last update:2022 04 21|External Id:SAMEA13254171|INSDC center alias:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC center name:Laboratory for Molecular Biodiscovery  KU Leuven  Campus Gasthuisberg|INSDC first public:2022 04 21T00:32:07Z|INSDC last update:2022 04 21T00:32:07Z|INSDC status:public|Submitter Id:E MTAB 11505:Sv2aHET2S3|age:6|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval stage|genotype:sv2a+/ |individual:pool of 10 heads|organism part:head|sample name:E MTAB 11505:Sv2aHET2S3|sex:not available|strain:AB", null, null, null, null, null, null, null, null, "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "E MTAB 11505:Sv2aHET2S3 s", "Sv2aHET2S3 s", "RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent Invitrogen  followed by phenol chlorophorm extraction and isopropanol precipitation. post ethanol washes  the RNA pellet was air dried  dissolved in nuclease free water Fermentas.  TruSeq RNA Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.", "Experimental Factor: genotype:sv2a+/ ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "SINGLE", "ILLUMINA", "NextSeq 500", null, "ERP135966", "NextSeq 500 sequencing; RNA seq experiment of sv2a /   sv2a+/  and sv2a+/+ zebrafish larvae", "ENA FIRST PUBLIC:2022 04 21|ENA LAST UPDATE:2022 04 21", "sv2aHET2S3L004-1.fastq.gz", "fastq", 747723481.0, 9898304.0, "E MTAB 11505:sv2aHET2S3L004", "0:75.54 1:0", "A:176935451;C:184684782;G:179046040;T:206946124;N:111084", 75, 0, null, null, 176935451, 184684782, 179046040, 206946124, 111084, "ERX8620326", "ERS10857214", "ERA9644804", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", "Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg|European Nucleotide Archive", 1, 0.95934, null, 0.08676, null, 0.65533, null, 0.47523, null, 75, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "Belgium", "2022-04-21", "Larval", "Larval", "Head", "Nervous System"], [32037, "SRR28992909", "SRX24520261", "SRS21267950", "SRP506997", "PRJNA1110229", "Zebrafish models of candidate human epilepsy associated genes provide evidence of hyperexcitability", "PRJNA1110229", "Other", "Hundreds of novel candidate human epilepsy associated genes have been identified thanks to advancements in next generation sequencing and large genome wide association studies  but establishing genetic etiology requires functional validation. We generated a list of >2200 candidate epilepsy associated genes  of which 81 were determined suitable for the generation of loss of function zebrafish models via CRISPR/Cas9 gene editing. Of those 81 crispants  48 were successfully established as stable mutant lines and assessed for seizure like swim patterns in a primary F2 screen. Evidence of seizure like behavior was present in 5 arfgef1  kcnd2  kcnv1  ubr5  wnt8b of the 48 mutant lines assessed. Further characterization of those 5 lines provided evidence for epileptiform activity via electrophysiology in kcnd2 and wnt8b mutants. Additionally  arfgef1 and wnt8b mutants showed a decrease in the number of inhibitory interneurons in the optic tectum of larval animals. Furthermore  RNAseq revealed convergent transcriptional abnormalities between mutant lines  consistent with their developmental defects and hyperexcitable phenotypes. These zebrafish models provide strongest experimental evidence supporting the role of ARFGEF1  KCND2  and WNT8B in human epilepsy and further demonstrate the utility of this model system for evaluating candidate human epilepsy genes.", null, null, null, null, "Kcnd2 Wt1", null, "strain:Casper|age:5dpf|collection date:2022 12 09|geo loc name:USA: Boston  MA|sex:not applicable|tissue:Head|genotype:Kcnd2 Wt1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio Rerio: pools of isolated larval head", "Kcnd2 Wt1", "Kcnd2 Wt1", "Three replicate larvae from Het in crosses  aged 5 dpf  were sacrificed on ice and stored in RNAlater Stabilization Solution according to product specifications. Larval heads were isolated from bodies  which were used for Sanger sequencing for genotyping. Heads were pooled in groups of 20 25 according to genotype and RNA was isolated using a Qiagen RNeasy plus kit according to specifications. QC was performed via Agilent 2100 bioanalyzer  and only samples with an RNA integrity number value of >5.8 were used for library preparation.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "SINGLE", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP506997", null, null, "Kcnd2_Wt1.fq.gz", "fastq", 3386455050.0, 22576367.0, "Kcnd2 Wt1.fq.gz", "0:150", "A:967137430;C:730108913;G:738646978;T:950549427;N:12302", 150, null, null, null, 967137430, 730108913, 738646978, 950549427, 12302, "SRX24520261", "SRS21267950", "SRA1863814", "Boston Childrens Hospital|Neurobiology", "Boston Childrens Hospital", null, null, null, null, null, null, null, null, null, null, null, "B", null, "usable mapping rate", "illumina", "novaseq_era", "unknown", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-05-10", "Larval", "Larval", "Head", "Nervous System"], [32038, "SRR28992910", "SRX24520260", "SRS21267949", "SRP506997", "PRJNA1110229", "Zebrafish models of candidate human epilepsy associated genes provide evidence of hyperexcitability", "PRJNA1110229", "Other", "Hundreds of novel candidate human epilepsy associated genes have been identified thanks to advancements in next generation sequencing and large genome wide association studies  but establishing genetic etiology requires functional validation. We generated a list of >2200 candidate epilepsy associated genes  of which 81 were determined suitable for the generation of loss of function zebrafish models via CRISPR/Cas9 gene editing. Of those 81 crispants  48 were successfully established as stable mutant lines and assessed for seizure like swim patterns in a primary F2 screen. Evidence of seizure like behavior was present in 5 arfgef1  kcnd2  kcnv1  ubr5  wnt8b of the 48 mutant lines assessed. Further characterization of those 5 lines provided evidence for epileptiform activity via electrophysiology in kcnd2 and wnt8b mutants. Additionally  arfgef1 and wnt8b mutants showed a decrease in the number of inhibitory interneurons in the optic tectum of larval animals. Furthermore  RNAseq revealed convergent transcriptional abnormalities between mutant lines  consistent with their developmental defects and hyperexcitable phenotypes. These zebrafish models provide strongest experimental evidence supporting the role of ARFGEF1  KCND2  and WNT8B in human epilepsy and further demonstrate the utility of this model system for evaluating candidate human epilepsy genes.", null, null, null, null, "Arfgef1 Hom3", null, "strain:Casper|age:5dpf|collection date:2022 12 09|geo loc name:USA: Boston  MA|sex:not applicable|tissue:Head|genotype:Arfgef1 Hom3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio Rerio: pools of isolated larval head", "Arfgef1 Hom3", "Arfgef1 Hom3", "Three replicate larvae from Het in crosses  aged 5 dpf  were sacrificed on ice and stored in RNAlater Stabilization Solution according to product specifications. Larval heads were isolated from bodies  which were used for Sanger sequencing for genotyping. Heads were pooled in groups of 20 25 according to genotype and RNA was isolated using a Qiagen RNeasy plus kit according to specifications. QC was performed via Agilent 2100 bioanalyzer  and only samples with an RNA integrity number value of >5.8 were used for library preparation.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "SINGLE", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP506997", null, null, "Arfgef1_Hom3.fq.gz", "fastq", 3647365500.0, 24315770.0, "Arfgef1 Hom3.fq.gz", "0:150", "A:923919033;C:903440333;G:911845870;T:908147370;N:12894", 150, null, null, null, 923919033, 903440333, 911845870, 908147370, 12894, "SRX24520260", "SRS21267949", "SRA1863814", "Boston Childrens Hospital|Neurobiology", "Boston Childrens Hospital", null, null, null, null, null, null, null, null, null, null, null, "B", null, "usable mapping rate", "illumina", "novaseq_era", "unknown", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-05-10", "Larval", "Larval", "Head", "Nervous System"], [32039, "SRR28992915", "SRX24520258", "SRS21267947", "SRP506997", "PRJNA1110229", "Zebrafish models of candidate human epilepsy associated genes provide evidence of hyperexcitability", "PRJNA1110229", "Other", "Hundreds of novel candidate human epilepsy associated genes have been identified thanks to advancements in next generation sequencing and large genome wide association studies  but establishing genetic etiology requires functional validation. We generated a list of >2200 candidate epilepsy associated genes  of which 81 were determined suitable for the generation of loss of function zebrafish models via CRISPR/Cas9 gene editing. Of those 81 crispants  48 were successfully established as stable mutant lines and assessed for seizure like swim patterns in a primary F2 screen. Evidence of seizure like behavior was present in 5 arfgef1  kcnd2  kcnv1  ubr5  wnt8b of the 48 mutant lines assessed. Further characterization of those 5 lines provided evidence for epileptiform activity via electrophysiology in kcnd2 and wnt8b mutants. Additionally  arfgef1 and wnt8b mutants showed a decrease in the number of inhibitory interneurons in the optic tectum of larval animals. Furthermore  RNAseq revealed convergent transcriptional abnormalities between mutant lines  consistent with their developmental defects and hyperexcitable phenotypes. These zebrafish models provide strongest experimental evidence supporting the role of ARFGEF1  KCND2  and WNT8B in human epilepsy and further demonstrate the utility of this model system for evaluating candidate human epilepsy genes.", null, null, null, null, "Arfgef1 Hom1", null, "strain:Casper|age:5dpf|collection date:2022 12 09|geo loc name:USA: Boston  MA|sex:not applicable|tissue:Head|genotype:Arfgef1 Hom1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio Rerio: pools of isolated larval head", "Arfgef1 Hom1", "Arfgef1 Hom1", "Three replicate larvae from Het in crosses  aged 5 dpf  were sacrificed on ice and stored in RNAlater Stabilization Solution according to product specifications. Larval heads were isolated from bodies  which were used for Sanger sequencing for genotyping. Heads were pooled in groups of 20 25 according to genotype and RNA was isolated using a Qiagen RNeasy plus kit according to specifications. QC was performed via Agilent 2100 bioanalyzer  and only samples with an RNA integrity number value of >5.8 were used for library preparation.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "SINGLE", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP506997", null, null, "Arfgef1_Hom1.fq.gz", "fastq", 3388869300.0, 22592462.0, "Arfgef1 Hom1.fq.gz", "0:150", "A:869162615;C:827706442;G:835453288;T:856534517;N:12438", 150, null, null, null, 869162615, 827706442, 835453288, 856534517, 12438, "SRX24520258", "SRS21267947", "SRA1863814", "Boston Childrens Hospital|Neurobiology", "Boston Childrens Hospital", null, null, null, null, null, null, null, null, null, null, null, "B", null, "usable mapping rate", "illumina", "novaseq_era", "unknown", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-05-10", "Larval", "Larval", "Head", "Nervous System"], [32040, "SRR28992912", "SRX24520257", "SRS21267946", "SRP506997", "PRJNA1110229", "Zebrafish models of candidate human epilepsy associated genes provide evidence of hyperexcitability", "PRJNA1110229", "Other", "Hundreds of novel candidate human epilepsy associated genes have been identified thanks to advancements in next generation sequencing and large genome wide association studies  but establishing genetic etiology requires functional validation. We generated a list of >2200 candidate epilepsy associated genes  of which 81 were determined suitable for the generation of loss of function zebrafish models via CRISPR/Cas9 gene editing. Of those 81 crispants  48 were successfully established as stable mutant lines and assessed for seizure like swim patterns in a primary F2 screen. Evidence of seizure like behavior was present in 5 arfgef1  kcnd2  kcnv1  ubr5  wnt8b of the 48 mutant lines assessed. Further characterization of those 5 lines provided evidence for epileptiform activity via electrophysiology in kcnd2 and wnt8b mutants. Additionally  arfgef1 and wnt8b mutants showed a decrease in the number of inhibitory interneurons in the optic tectum of larval animals. Furthermore  RNAseq revealed convergent transcriptional abnormalities between mutant lines  consistent with their developmental defects and hyperexcitable phenotypes. These zebrafish models provide strongest experimental evidence supporting the role of ARFGEF1  KCND2  and WNT8B in human epilepsy and further demonstrate the utility of this model system for evaluating candidate human epilepsy genes.", null, null, null, null, "Arfgef1 Het3", null, "strain:Casper|age:5dpf|collection date:2022 12 09|geo loc name:USA: Boston  MA|sex:not applicable|tissue:Head|genotype:Arfgef1 Het3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio Rerio: pools of isolated larval head", "Arfgef1 Het3", "Arfgef1 Het3", "Three replicate larvae from Het in crosses  aged 5 dpf  were sacrificed on ice and stored in RNAlater Stabilization Solution according to product specifications. Larval heads were isolated from bodies  which were used for Sanger sequencing for genotyping. Heads were pooled in groups of 20 25 according to genotype and RNA was isolated using a Qiagen RNeasy plus kit according to specifications. QC was performed via Agilent 2100 bioanalyzer  and only samples with an RNA integrity number value of >5.8 were used for library preparation.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "SINGLE", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP506997", null, null, "Arfgef1_Het3.fq.gz", "fastq", 3369996750.0, 22466645.0, "Arfgef1 Het3.fq.gz", "0:150", "A:861941573;C:824864964;G:833793356;T:849388791;N:8066", 150, null, null, null, 861941573, 824864964, 833793356, 849388791, 8066, "SRX24520257", "SRS21267946", "SRA1863814", "Boston Childrens Hospital|Neurobiology", "Boston Childrens Hospital", null, null, null, null, null, null, null, null, null, null, null, "B", null, "usable mapping rate", "illumina", "novaseq_era", "unknown", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-05-10", "Larval", "Larval", "Head", "Nervous System"], [32041, "SRR28992913", "SRX24520256", "SRS21267945", "SRP506997", "PRJNA1110229", "Zebrafish models of candidate human epilepsy associated genes provide evidence of hyperexcitability", "PRJNA1110229", "Other", "Hundreds of novel candidate human epilepsy associated genes have been identified thanks to advancements in next generation sequencing and large genome wide association studies  but establishing genetic etiology requires functional validation. We generated a list of >2200 candidate epilepsy associated genes  of which 81 were determined suitable for the generation of loss of function zebrafish models via CRISPR/Cas9 gene editing. Of those 81 crispants  48 were successfully established as stable mutant lines and assessed for seizure like swim patterns in a primary F2 screen. Evidence of seizure like behavior was present in 5 arfgef1  kcnd2  kcnv1  ubr5  wnt8b of the 48 mutant lines assessed. Further characterization of those 5 lines provided evidence for epileptiform activity via electrophysiology in kcnd2 and wnt8b mutants. Additionally  arfgef1 and wnt8b mutants showed a decrease in the number of inhibitory interneurons in the optic tectum of larval animals. Furthermore  RNAseq revealed convergent transcriptional abnormalities between mutant lines  consistent with their developmental defects and hyperexcitable phenotypes. These zebrafish models provide strongest experimental evidence supporting the role of ARFGEF1  KCND2  and WNT8B in human epilepsy and further demonstrate the utility of this model system for evaluating candidate human epilepsy genes.", null, null, null, null, "Arfgef1 Het2", null, "strain:Casper|age:5dpf|collection date:2022 12 09|geo loc name:USA: Boston  MA|sex:not applicable|tissue:Head|genotype:Arfgef1 Het2|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio Rerio: pools of isolated larval head", "Arfgef1 Het2", "Arfgef1 Het2", "Three replicate larvae from Het in crosses  aged 5 dpf  were sacrificed on ice and stored in RNAlater Stabilization Solution according to product specifications. Larval heads were isolated from bodies  which were used for Sanger sequencing for genotyping. Heads were pooled in groups of 20 25 according to genotype and RNA was isolated using a Qiagen RNeasy plus kit according to specifications. QC was performed via Agilent 2100 bioanalyzer  and only samples with an RNA integrity number value of >5.8 were used for library preparation.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "SINGLE", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP506997", null, null, "Arfgef1_Het2.fq.gz", "fastq", 4755406500.0, 31702710.0, "Arfgef1 Het2.fq.gz", "0:150", "A:1254995507;C:1127923454;G:1138127034;T:1234180808;N:179697", 150, null, null, null, 1254995507, 1127923454, 1138127034, 1234180808, 179697, "SRX24520256", "SRS21267945", "SRA1863814", "Boston Childrens Hospital|Neurobiology", "Boston Childrens Hospital", null, null, null, null, null, null, null, null, null, null, null, "B", null, "usable mapping rate", "illumina", "novaseq_era", "unknown", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-05-10", "Larval", "Larval", "Head", "Nervous System"], [32042, "SRR28992916", "SRX24520254", "SRS21267943", "SRP506997", "PRJNA1110229", "Zebrafish models of candidate human epilepsy associated genes provide evidence of hyperexcitability", "PRJNA1110229", "Other", "Hundreds of novel candidate human epilepsy associated genes have been identified thanks to advancements in next generation sequencing and large genome wide association studies  but establishing genetic etiology requires functional validation. We generated a list of >2200 candidate epilepsy associated genes  of which 81 were determined suitable for the generation of loss of function zebrafish models via CRISPR/Cas9 gene editing. Of those 81 crispants  48 were successfully established as stable mutant lines and assessed for seizure like swim patterns in a primary F2 screen. Evidence of seizure like behavior was present in 5 arfgef1  kcnd2  kcnv1  ubr5  wnt8b of the 48 mutant lines assessed. Further characterization of those 5 lines provided evidence for epileptiform activity via electrophysiology in kcnd2 and wnt8b mutants. Additionally  arfgef1 and wnt8b mutants showed a decrease in the number of inhibitory interneurons in the optic tectum of larval animals. Furthermore  RNAseq revealed convergent transcriptional abnormalities between mutant lines  consistent with their developmental defects and hyperexcitable phenotypes. These zebrafish models provide strongest experimental evidence supporting the role of ARFGEF1  KCND2  and WNT8B in human epilepsy and further demonstrate the utility of this model system for evaluating candidate human epilepsy genes.", null, null, null, null, "Wnt8b Hom3", null, "strain:Casper|age:5dpf|collection date:2022 12 09|geo loc name:USA: Boston  MA|sex:not applicable|tissue:Head|genotype:Wnt8b Hom3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio Rerio: pools of isolated larval head", "Wnt8b Hom3", "Wnt8b Hom3", "Three replicate larvae from Het in crosses  aged 5 dpf  were sacrificed on ice and stored in RNAlater Stabilization Solution according to product specifications. Larval heads were isolated from bodies  which were used for Sanger sequencing for genotyping. Heads were pooled in groups of 20 25 according to genotype and RNA was isolated using a Qiagen RNeasy plus kit according to specifications. QC was performed via Agilent 2100 bioanalyzer  and only samples with an RNA integrity number value of >5.8 were used for library preparation.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "SINGLE", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP506997", null, null, "Wnt8b_Hom3.fq.gz", "fastq", 5283928950.0, 35226193.0, "Wnt8b Hom3.fq.gz", "0:150", "A:1389228037;C:1262004726;G:1265392219;T:1367105548;N:198420", 150, null, null, null, 1389228037, 1262004726, 1265392219, 1367105548, 198420, "SRX24520254", "SRS21267943", "SRA1863814", "Boston Childrens Hospital|Neurobiology", "Boston Childrens Hospital", null, null, null, null, null, null, null, null, null, null, null, "B", null, "usable mapping rate", "illumina", "novaseq_era", "unknown", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-05-10", "Larval", "Larval", "Head", "Nervous System"], [32043, "SRR28992919", "SRX24520251", "SRS21267940", "SRP506997", "PRJNA1110229", "Zebrafish models of candidate human epilepsy associated genes provide evidence of hyperexcitability", "PRJNA1110229", "Other", "Hundreds of novel candidate human epilepsy associated genes have been identified thanks to advancements in next generation sequencing and large genome wide association studies  but establishing genetic etiology requires functional validation. We generated a list of >2200 candidate epilepsy associated genes  of which 81 were determined suitable for the generation of loss of function zebrafish models via CRISPR/Cas9 gene editing. Of those 81 crispants  48 were successfully established as stable mutant lines and assessed for seizure like swim patterns in a primary F2 screen. Evidence of seizure like behavior was present in 5 arfgef1  kcnd2  kcnv1  ubr5  wnt8b of the 48 mutant lines assessed. Further characterization of those 5 lines provided evidence for epileptiform activity via electrophysiology in kcnd2 and wnt8b mutants. Additionally  arfgef1 and wnt8b mutants showed a decrease in the number of inhibitory interneurons in the optic tectum of larval animals. Furthermore  RNAseq revealed convergent transcriptional abnormalities between mutant lines  consistent with their developmental defects and hyperexcitable phenotypes. These zebrafish models provide strongest experimental evidence supporting the role of ARFGEF1  KCND2  and WNT8B in human epilepsy and further demonstrate the utility of this model system for evaluating candidate human epilepsy genes.", null, null, null, null, "Wnt8b Het3", null, "strain:Casper|age:5dpf|collection date:2022 12 09|geo loc name:USA: Boston  MA|sex:not applicable|tissue:Head|genotype:Wnt8b Het3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio Rerio: pools of isolated larval head", "Wnt8b Het3", "Wnt8b Het3", "Three replicate larvae from Het in crosses  aged 5 dpf  were sacrificed on ice and stored in RNAlater Stabilization Solution according to product specifications. Larval heads were isolated from bodies  which were used for Sanger sequencing for genotyping. Heads were pooled in groups of 20 25 according to genotype and RNA was isolated using a Qiagen RNeasy plus kit according to specifications. QC was performed via Agilent 2100 bioanalyzer  and only samples with an RNA integrity number value of >5.8 were used for library preparation.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "SINGLE", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP506997", null, null, "Wnt8b_Het3.fq.gz", "fastq", 4357330950.0, 29048873.0, "Wnt8b Het3.fq.gz", "0:150", "A:1179652667;C:1002955689;G:1008147493;T:1166494534;N:80567", 150, null, null, null, 1179652667, 1002955689, 1008147493, 1166494534, 80567, "SRX24520251", "SRS21267940", "SRA1863814", "Boston Childrens Hospital|Neurobiology", "Boston Childrens Hospital", null, null, null, null, null, null, null, null, null, null, null, "B", null, "usable mapping rate", "illumina", "novaseq_era", "unknown", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United States", "2024-05-10", "Larval", "Larval", "Head", "Nervous System"], [34964, "SRR32588721", "SRX27895230", "SRS24266233", "SRP568323", "PRJNA1232602", "Telomere to telomere genome assemblies for commonly used zebrafish laboratory strains", "PRJNA1232602", "Other", "In this study  Iso Seq was performed on different zebrafish body organs  enabling a comprehensive view of organ specific transcriptomes. High quality PacBio Iso Seq long reads were generated from key zebrafish tissues  including the brain  testis  liver  eye  muscle  ovary  inner ear and kidney  to identify novel isoforms  tissue specific transcripts  and alternative splicing events. We also made available the Iso Seq data from embryos from different time points  hpf  0  6  12  and 24. This dataset enhances gene annotation  improves reference genome annotations  and provides insights into zebrafish organ specific gene expression.", null, null, null, "Iso Seq RNA from Danio rerio", "C2 F2 F B", null, "strain:NHGRI2|isolate:Single paired cross|breed:Zebrafish|cultivar:wild type|ecotype:United States|age:9 month|dev stage:adult fish|collection date:2024 06|geo loc name:USA|sex:female|tissue:brain|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Iso Seq RNA from brain", "C2 F2 F B", "C2 F2 F B", "The long Read Sequencing libraries was sequenced using PacBio Sequel II", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "SINGLE", "PACBIO_SMRT", "Sequel II", null, "SRP568323", null, null, "m64467e_240523_204656.skera.flnc.fastq.gz", "fastq", 11267908778.0, 6337509.0, "m64467e 240523 204656.skera.flnc.fastq.gz", "0:1777.97", "A:3374083285;C:2369790412;G:2399920883;T:3124114198;N:0", 1777, null, null, null, 3374083285, 2369790412, 2399920883, 3124114198, 0, "SRX27895230", "SRS24266233", "SRA2089085", "National Human Genome Research Institute|Translational and Functional Genomics Branch", "National Human Genome Research Institute National Human Genome Research Institute", null, null, null, null, null, null, null, null, null, null, null, "T", null, "long read", "pacbio", "pacbio_modern", "unknown", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United States", "2025-03-06", "Adult", "Adult", "Brain", "Nervous System"], [34973, "SRR32588730", "SRX27895221", "SRS24266225", "SRP568323", "PRJNA1232602", "Telomere to telomere genome assemblies for commonly used zebrafish laboratory strains", "PRJNA1232602", "Other", "In this study  Iso Seq was performed on different zebrafish body organs  enabling a comprehensive view of organ specific transcriptomes. High quality PacBio Iso Seq long reads were generated from key zebrafish tissues  including the brain  testis  liver  eye  muscle  ovary  inner ear and kidney  to identify novel isoforms  tissue specific transcripts  and alternative splicing events. We also made available the Iso Seq data from embryos from different time points  hpf  0  6  12  and 24. This dataset enhances gene annotation  improves reference genome annotations  and provides insights into zebrafish organ specific gene expression.", null, null, null, "Iso Seq RNA from Danio rerio", "C2 F2 M B", null, "strain:NHGRI2|isolate:Single paired cross|breed:Zebrafish|cultivar:wild type|ecotype:United States|age:9 month|dev stage:adult fish|collection date:2024 06|geo loc name:USA|sex:male|tissue:brain|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Iso Seq RNA from brain", "C2 F2 M B", "C2 F2 M B", "The long Read Sequencing libraries was sequenced using PacBio Sequel II", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "SINGLE", "PACBIO_SMRT", "Sequel II", null, "SRP568323", null, null, "m84270_240904_192359_s4.skera.flnc.fastq.gz", "fastq", 64391727308.0, 29612753.0, "m84270 240904 192359 s4.skera.flnc.fastq.gz", "0:2174.46", "A:18972670876;C:13388478305;G:13738034427;T:18292543700;N:0", 2174, null, null, null, 18972670876, 13388478305, 13738034427, 18292543700, 0, "SRX27895221", "SRS24266225", "SRA2089085", "National Human Genome Research Institute|Translational and Functional Genomics Branch", "National Human Genome Research Institute National Human Genome Research Institute", null, null, null, null, null, null, null, null, null, null, null, "T", null, "long read", "pacbio", "pacbio_modern", "unknown", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United States", "2025-03-06", "Adult", "Adult", "Brain", "Nervous System"], [35869, "SRR33094451", "SRX28358126", "SRS24687216", "SRP578075", "PRJNA1249535", "P301S Mutant Tau Driven Zebrafish Platform Enables Systematic Identification of Pharmacological GSK3B Inhibitors with Anti Neurodegenerative Efficacy", "PRJNA1249535", "Other", "Single cell transcriptome sequencing provides high resolution transcriptome data on the molecular mechanisms by which Tau P301S mediates Alzheimer's disease like pathological changes in zebrafish  providing unique insights into the pathogenesis of Tau pathology mediated cell to cell interactions in AD.", null, null, "Wildtype2", null, "Wildtype2", null, "breed:zebrafish|age:4 month|collection date:2024 05 14|geo loc name:China:Shantou|sex:not determined|tissue:brain|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "P301S Mutant Tau Driven Zebrafish Platform Enables Systematic Identification of Pharmacological GSK3B Inhibitors with Anti Neurodegenerative Efficacy", "Wildtype2", "Wildtype2", "tissue", null, null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "PolyA", "SINGLE", "BGISEQ", "BGISEQ-500", null, "SRP578075", null, null, "WT2_S1_L001_I1_001.fastq.gz", "fastq", 15183539264.0, 1897942408.0, "WT2 S1 L001 I1 001.fastq.gz", "0:8", "A:5024041949;C:2632487194;G:2782260146;T:4744243552;N:506423", 8, null, null, null, 5024041949, 2632487194, 2782260146, 4744243552, 506423, "SRX28358126", "SRS24687216", "SRA2110625", "Shantou university medical college|Neuroscience Center", "Shantou university medical college", null, null, null, null, null, null, null, null, null, null, null, "T", null, "under 1.2% mapping rate", "bgi", "bgi", "unknown", "poly_a", "unknown", "sc", "unknown", "unknown", null, "China", "2025-04-18", "Adult", "Adult", "Brain", "Nervous System"], [35870, "SRR33094452", "SRX28358125", "SRS24687217", "SRP578075", "PRJNA1249535", "P301S Mutant Tau Driven Zebrafish Platform Enables Systematic Identification of Pharmacological GSK3B Inhibitors with Anti Neurodegenerative Efficacy", "PRJNA1249535", "Other", "Single cell transcriptome sequencing provides high resolution transcriptome data on the molecular mechanisms by which Tau P301S mediates Alzheimer's disease like pathological changes in zebrafish  providing unique insights into the pathogenesis of Tau pathology mediated cell to cell interactions in AD.", null, null, "Wildtype1", null, "Wildtype1", null, "breed:zebrafish|age:4 month|collection date:2024 05 13|geo loc name:China:Shantou|sex:not determined|tissue:brain|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "P301S Mutant Tau Driven Zebrafish Platform Enables Systematic Identification of Pharmacological GSK3B Inhibitors with Anti Neurodegenerative Efficacy", "Wildtype1", "Wildtype1", "tissue", null, null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "PolyA", "SINGLE", "BGISEQ", "BGISEQ-500", null, "SRP578075", null, null, "WT1_S1_L001_I1_001.fastq.gz", "fastq", 7991376264.0, 998922033.0, "WT1 S1 L001 I1 001.fastq.gz", "0:8", "A:2573734851;C:1426474966;G:1492966198;T:2498173074;N:27175", 8, null, null, null, 2573734851, 1426474966, 1492966198, 2498173074, 27175, "SRX28358125", "SRS24687217", "SRA2110625", "Shantou university medical college|Neuroscience Center", "Shantou university medical college", null, null, null, null, null, null, null, null, null, null, null, "T", null, "under 1.2% mapping rate", "bgi", "bgi", "unknown", "poly_a", "unknown", "sc", "unknown", "unknown", null, "China", "2025-04-18", "Adult", "Adult", "Brain", "Nervous System"], [36363, "SRR489486", "SRX143563", "SRS310284", "SRP012376", "PRJNA160143", "Extensive alternative polyadenylation during zebrafish development", "GSE37453", "Transcriptome Analysis", "The post transcriptional fate of messenger RNAs mRNAs is largely dictated by their three prime' untranslated regions three prime'UTRs  which are defined by cleavage and polyadenylation CPA of pre mRNAs. We used polyA position profiling by sequencing 3P Seq to map polyA sites at eight developmental stages and tissues in the zebrafish. Analysis of over 60 million 3P Seq reads substantially increased and improved existing three prime'UTR annotations  resulting in confidently identified three prime'UTRs for more than 78.79% of the annotated protein coding genes in zebrafish. Most zebrafish genes undergo alternative CPA with more than a thousand genes using different dominant three prime'UTRs at different stages. three prime'UTRs tend to be shortest in the ovaries and longest in the brain. Isoforms with some of the shortest three prime'UTRs are highly expressed in the ovary yet absent in the maternally contributed RNAs of the embryo  perhaps because their three prime'UTRs are too short to accommodate a uridine rich motif required for stability of the maternal mRNA. At two hpf  thousands of unique polyA sites appear at locations lacking a typical polyadenylation signal  which suggests a wave of widespread cytoplasmic polyadenylation of mRNA degradation intermediates. Our insights into the identities  formation  and evolution of zebrafish three prime'UTRs provide a resource for studying gene regulation during vertebrate development. Overall design: 3P Seq was used to map the three prime' ends of protein coding genes in the zebrafish genome", null, "pubmed:22722342", null, "3P Seq Brain", "GSM919969", null, "source name:mixed gender adults|genotype/variation:wild type|tissue:brain|development stage:adult", "3P Seq Brain", "For 3P Seq: Reads were reverse complemented and aligned to the D. rerio genome Zv9/danRer7 using Bowtie. Reads that aligned to up to four genomic locus and had one or more mismatches at their three prime end within a terminal adenylate run were carried forward as 3P tags. Reads mapping to the same locus with the same number of terminal adenylates were consolidated in the processed data file. The BED file is as in Jan et al. GSE24924 For 3P PE Seq: In each read pair  read #1 captured the three prime end of the polyA tail in the antisense orientation  and read #2 captured a portion of the three prime region of the transcript  and occasionally  the beginning of the polyA tail  in the sense orientation. Read #2 began with an adapter of 26 bases. Reads with more than 10 mismatches to the adapter were discarded and the first 26 bases were removed before further processing.  A read pair was considered informative only if read #1 began with Ts and read #2 contained 2\u201339 terminal As. post leading Ts and terminal As were removed from reads #1 and #2  respectively  they were mapped to the genome using Bowtie  allowing for up to two mismatches and requiring a unique mapping position in the zebrafish genome. The length of the tail encoded in read #2 was defined as the maximum number of trailing As allowing for up to one mismatch. Only cases in which this number was larger than the number of As encoded in the genome at the predicted cleavage position by at least 2 bases were carried forward. Cleavage and polyadenylation position was defined as the last non A base in read #2. The length of the polyA tail at that position was estimated using the corresponding read #1  and defined as the maximal i for which >90% of the bases in the first i bases of read #1 were Ts. This criterion was used to allow for some sequencing errors expected when sequencing long homopolymers. Genome build: danRer7 Supplementary files format and content: The .bed files contain the positions where 3P Seq reads were mapped  and the name of each track is the number of reads mapping to that position. Supplementary files format and content: The bigWig .bw files contain a summary of the number of reads whose three prime ends mapped to each position in the genome. Supplementary files format and content: For the paired end sequencing for mapping polyA tail lengths  the text files contain the following columns : chromosome  position  strand  number of polyA length reads  followed by the individual measurements lower bounds on polyA tail length", "mixed gender adults", "Anesthetized decorioneted embryos were washed three times in PBS 137 mM NaCl  2.7 mM KCl  1.5 mM KH2PO4  8 mM Na2HP04  pH 7.4 and suspended in PBS containing 1% freshly added formaldehyde. Embryos were transferred to a dounce homogenizer  dounced several times and incubated at room temperature for 15 min. Formaldehyde was quenched by adding 1/20 volume 2.5 M glycine. Cells were pelleted at 400 x g for 5 min. The supernatant was removed  and pellets were rinsed twice with PBS  flash frozen in liquid nitrogen and stored at 80C.", "3P Seq; see http://web.wi.mit.edu/bartel/pub/protocols.html", "Zebrafish embryos or adults grown under standard condition", "genotype/variation:wild type|tissue:brain|developmental stage:adult", "GSM919969", "GSM919969: 3P Seq Brain; Danio rerio; RNA Seq", "GSM919969 1", "GSM919969: 3P Seq Brain", "1", null, "GEO Accession:GSM919969", "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "SINGLE", "ILLUMINA", "Illumina Genome Analyzer II", "<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>36</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>", "SRP012376", null, null, "3P_Seq_Brain.fastq", "fastq", 592119324.0, 16447759.0, "GSM919969 r1", "0:36", "A:231026484;C:109822201;G:93671127;T:157421018;N:178494", 36, null, null, null, 231026484, 109822201, 93671127, 157421018, 178494, "SRX143563", "SRS310284", "SRA051955", "GEO", "Whitehead Institute for Biomedical Research", 1, 0.57742, null, 0.10125, null, 0.78267, null, 0.52634, null, 36, null, "B", null, "usable mapping rate", "illumina", "early_illumina", "3prime", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United States", "2012-04-20", "Adult", "Adult", "Brain", "Nervous System"], [36479, "SRR527834", "SRX171221", "SRS352107", "SRP014596", "PRJNA171539", "Transcriptomic analysis of zebrafish during development and homeostasis", "GSE39703", "Transcriptome Analysis", "Sequencing libraries were generated from total RNA samples following the mRNAseq protocol for the generation of single end 16 hpf 36 hpf  5 day larvae  adult head and adult tail or paired end 24 hpf libraries Illumina. Single end reads of 36 nucleotides and paired end reads 2 x 76 nucleotides were obtained with a GAIIx Illumina. Gene expression at the different stages/tissu was assessed by cufflinks and HTseq. Overall design: RNAseq on 5 differents samples:  24hpf embryos  pool of 16 hour to 36 hour embryos  5 dpf larvea  adult head and adult tail", null, "pubmed:23684812", null, "head", "GSM977959", null, "tissue:entire adult head|genotype:Wild type|strain:AB|Stage:adult head", "head", "Basecalls performed using CASAVA version 1.4 Reads aligned to zebrafish genome Zv9 Ensembl with Tophat v 1.4.1 and Bowtie v 0.12.7  options: butterfly search coverage search  microexon search  min anchor length 5   G GTF Determination of raw reads aligned to ZV9 with HTSeq v0.5.3p3 and gtf file ensembl zv9 release 60 Determination of RPKM/FPKM with cufflinks v 1.3.0 with the options  u  b  M rRNA/Mtgenes mask  G gtf Genome build: Zv9 v 60 Supplementary files format and content: *.count file are the output of Htseq reads quantification. *.fpkm files are the output of cufflinks quantification", "entire adult head", null, "Extraction of total RNA with Trizol following manufacturer's instruction. Generation of mRNA libraries with Trueseq RNA kit following Illumina's instructions", null, "genotype:Wild type|strain:AB|Stage:adult head", "GSM977959", "GSM977959: head; Danio rerio; RNA Seq", "GSM977959 1", "GSM977959: head", "1", null, "GEO Accession:GSM977959", "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "SINGLE", "ILLUMINA", "Illumina Genome Analyzer IIx", "<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>36</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>", "SRP014596", null, null, null, null, 516054600.0, 14334850.0, "GSM977959 r1", "0:36", "A:144148482;C:115800670;G:121205261;T:134746787;N:153400", 36, null, null, null, 144148482, 115800670, 121205261, 134746787, 153400, "SRX171221", "SRS352107", "SRA056408", "GEO", "ITG", 1, 0.89668, null, 0.12577, null, 0.66803, null, 0.45871, null, 36, null, "B", null, "usable mapping rate", "illumina", "early_illumina", "unknown", "cdna_unspecified", "trueseq", "bulk", "unknown", "unknown", null, "Unknown", "2012-07-27", "Adult", "Adult", "Head", "Nervous System"], [37924, "SRR1554486", "SRX685392", "SRS686641", "SRP039502", "PRJNA240316", "Danio rerio strain:AB Transcriptome or Gene expression", "PRJNA240316", "Transcriptome Analysis", "Quantifying expression levels of smallRNAs between tissues in Danio Rerio strain AB.", null, "pubmed:24835514", null, null, "ZF2Brain1", null, "strain:AB|age:Reproductive Adult|biomaterial provider:Postlethwait Lab Institute of Neuroscience 1254 University of Oregon 222 Huestis Hall Eugene OR  97407|sex:male|tissue:Brain|birth location:University of Oregon Fish Facility|collected by:Thomas Desvignes|collection date:2013 10 07|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Zebrafish Brain2", "ZF2Brain2", "1", "Tissue specific sequencing libraries were prepped and barcoded using the BiooScientific NEXTflex smallRNA Sequencing Kit  which uses a 3\u2019 adenylated adapter that ligates onto miRs and other small RNAs with a 3\u2019 hydroxyl group.", null, null, "miRNA-Seq", "TRANSCRIPTOMIC", "unspecified", "SINGLE", "ILLUMINA", "Illumina HiSeq 2500", "<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>101</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>", "SRP039502", null, null, "ZF2_Brain_3.fq.gz", "fastq", 1211892536.0, 11998936.0, "Brain2 Run3", "0:101", "A:374228993;C:286134640;G:253944548;T:297038575;N:545780", 101, null, null, null, 374228993, 286134640, 253944548, 297038575, 545780, "SRX685392", "SRS686641", "SRA142461", "University of Oregon|JHP-Lab", "University of Oregon", 1, 0.0, null, 0.0, null, 1.0, null, null, null, 101, null, "T", null, "under 1.2% mapping rate", "illumina", "hiseq_era", "unknown", "small_rna", "unknown", "bulk", "unknown", "unknown", null, "United States", "2014-08-22", "Adult", "Adult", "Brain", "Nervous System"], [37928, "SRR1554474", "SRX685388", "SRS566490", "SRP039502", "PRJNA240316", "Danio rerio strain:AB Transcriptome or Gene expression", "PRJNA240316", "Transcriptome Analysis", "Quantifying expression levels of smallRNAs between tissues in Danio Rerio strain AB.", null, "pubmed:24835514", null, null, "ZF1Brain1", null, "strain:AB|age:Reproductive Adult|biomaterial provider:Postlethwait Lab Institute of Neuroscience 1254 University of Oregon 222 Huestis Hall Eugene OR  97403|sex:male|tissue:Brain|birth location:University of Oregon Fish Facility|collected by:Thomas Desvignes|collection date:2013 10 07|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Zebrafish Brain1", "ZFBrain1 2", "1", "Tissue specific sequencing libraries were prepped and barcoded using the BiooScientific NEXTflex smallRNA Sequencing Kit  which uses a 3\u2019 adenylated adapter that ligates onto miRs and other small RNAs with a 3\u2019 hydroxyl group.", null, null, "miRNA-Seq", "TRANSCRIPTOMIC", "unspecified", "SINGLE", "ILLUMINA", "Illumina HiSeq 2500", "<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>101</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>", "SRP039502", null, null, "ZF1_Brain_3.fq.gz", "fastq", 1192445794.0, 11806394.0, "Brain1 Run3", "0:101", "A:369786121;C:268557682;G:265557417;T:288006805;N:537769", 101, null, null, null, 369786121, 268557682, 265557417, 288006805, 537769, "SRX685388", "SRS566490", "SRA142461", "University of Oregon|JHP-Lab", "University of Oregon", 1, 2e-05, null, 1e-05, null, 1.0, null, null, null, 101, null, "T", null, "under 1.2% mapping rate", "illumina", "hiseq_era", "unknown", "small_rna", "unknown", "bulk", "unknown", "unknown", null, "United States", "2014-08-22", "Adult", "Adult", "Brain", "Nervous System"], [37935, "SRR1554484", "SRX683268", "SRS686641", "SRP039502", "PRJNA240316", "Danio rerio strain:AB Transcriptome or Gene expression", "PRJNA240316", "Transcriptome Analysis", "Quantifying expression levels of smallRNAs between tissues in Danio Rerio strain AB.", null, "pubmed:24835514", null, null, "ZF2Brain1", null, "strain:AB|age:Reproductive Adult|biomaterial provider:Postlethwait Lab Institute of Neuroscience 1254 University of Oregon 222 Huestis Hall Eugene OR  97407|sex:male|tissue:Brain|birth location:University of Oregon Fish Facility|collected by:Thomas Desvignes|collection date:2013 10 07|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Zebrafish Brain2", "ZF2Brain1", "1", "Tissue specific sequencing libraries were prepped and barcoded using the BiooScientific NEXTflex smallRNA Sequencing Kit  which uses a 3\u2019 adenylated adapter that ligates onto miRs and other small RNAs with a 3\u2019 hydroxyl group.", null, null, "miRNA-Seq", "TRANSCRIPTOMIC", "unspecified", "SINGLE", "ILLUMINA", "Illumina HiSeq 2500", "<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>51</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>", "SRP039502", null, null, "ZF2_Brain_1.fq.gz", "fastq", 654412671.0, 12831621.0, "Brain2 Run1", "0:51", "A:160544180;C:166355290;G:168608762;T:158684308;N:220131", 51, null, null, null, 160544180, 166355290, 168608762, 158684308, 220131, "SRX683268", "SRS686641", "SRA142461", "University of Oregon|JHP-Lab", "University of Oregon", 1, 3e-05, null, 0.0, null, 0.99993, null, 1.0, null, 51, null, "T", null, "under 1.2% mapping rate", "illumina", "hiseq_era", "unknown", "small_rna", "unknown", "bulk", "unknown", "unknown", null, "United States", "2014-08-22", "Adult", "Adult", "Brain", "Nervous System"], [37936, "SRR1554485", "SRX683268", "SRS686641", "SRP039502", "PRJNA240316", "Danio rerio strain:AB Transcriptome or Gene expression", "PRJNA240316", "Transcriptome Analysis", "Quantifying expression levels of smallRNAs between tissues in Danio Rerio strain AB.", null, "pubmed:24835514", null, null, "ZF2Brain1", null, "strain:AB|age:Reproductive Adult|biomaterial provider:Postlethwait Lab Institute of Neuroscience 1254 University of Oregon 222 Huestis Hall Eugene OR  97407|sex:male|tissue:Brain|birth location:University of Oregon Fish Facility|collected by:Thomas Desvignes|collection date:2013 10 07|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Zebrafish Brain2", "ZF2Brain1", "1", "Tissue specific sequencing libraries were prepped and barcoded using the BiooScientific NEXTflex smallRNA Sequencing Kit  which uses a 3\u2019 adenylated adapter that ligates onto miRs and other small RNAs with a 3\u2019 hydroxyl group.", null, null, "miRNA-Seq", "TRANSCRIPTOMIC", "unspecified", "SINGLE", "ILLUMINA", "Illumina HiSeq 2500", "<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>51</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>", "SRP039502", null, null, "ZF2_Brain_2.fq.gz", "fastq", 856322793.0, 16790643.0, "Brain2 Run2", "0:51", "A:210706815;C:226772546;G:212404752;T:201722093;N:4716587", 51, null, null, null, 210706815, 226772546, 212404752, 201722093, 4716587, "SRX683268", "SRS686641", "SRA142461", "University of Oregon|JHP-Lab", "University of Oregon", 1, 0.0, null, 0.0, null, 1.0, null, null, null, 51, null, "T", null, "under 1.2% mapping rate", "illumina", "hiseq_era", "unknown", "small_rna", "unknown", "bulk", "unknown", "unknown", null, "United States", "2014-08-22", "Adult", "Adult", "Brain", "Nervous System"], [37943, "SRR1554472", "SRX481992", "SRS566490", "SRP039502", "PRJNA240316", "Danio rerio strain:AB Transcriptome or Gene expression", "PRJNA240316", "Transcriptome Analysis", "Quantifying expression levels of smallRNAs between tissues in Danio Rerio strain AB.", null, "pubmed:24835514", null, null, "ZF1Brain1", null, "strain:AB|age:Reproductive Adult|biomaterial provider:Postlethwait Lab Institute of Neuroscience 1254 University of Oregon 222 Huestis Hall Eugene OR  97403|sex:male|tissue:Brain|birth location:University of Oregon Fish Facility|collected by:Thomas Desvignes|collection date:2013 10 07|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Zebrafish Brain1", "ZFBrain1 1", "1", "Tissue specific sequencing libraries were prepped and barcoded using the BiooScientific NEXTflex smallRNA Sequencing Kit  which uses a 3\u2019 adenylated adapter that ligates onto miRs and other small RNAs with a 3\u2019 hydroxyl group.", null, null, "miRNA-Seq", "TRANSCRIPTOMIC", "unspecified", "SINGLE", "ILLUMINA", "Illumina HiSeq 2500", "<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>51</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>", "SRP039502", null, null, "ZF1_Brain_1.fq.gz", "fastq", 679860294.0, 13330594.0, "Brain1 Run1", "0:51", "A:167202435;C:176450132;G:174468721;T:161497185;N:241821", 51, null, null, null, 167202435, 176450132, 174468721, 161497185, 241821, "SRX481992", "SRS566490", "SRA142461", "University of Oregon|JHP-Lab", "University of Oregon", 1, 0.0, null, 0.0, null, 1.0, null, null, null, 51, null, "T", null, "under 1.2% mapping rate", "illumina", "hiseq_era", "unknown", "small_rna", "unknown", "bulk", "unknown", "unknown", null, "United States", "2014-08-22", "Adult", "Adult", "Brain", "Nervous System"], [37944, "SRR1554473", "SRX481992", "SRS566490", "SRP039502", "PRJNA240316", "Danio rerio strain:AB Transcriptome or Gene expression", "PRJNA240316", "Transcriptome Analysis", "Quantifying expression levels of smallRNAs between tissues in Danio Rerio strain AB.", null, "pubmed:24835514", null, null, "ZF1Brain1", null, "strain:AB|age:Reproductive Adult|biomaterial provider:Postlethwait Lab Institute of Neuroscience 1254 University of Oregon 222 Huestis Hall Eugene OR  97403|sex:male|tissue:Brain|birth location:University of Oregon Fish Facility|collected by:Thomas Desvignes|collection date:2013 10 07|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Zebrafish Brain1", "ZFBrain1 1", "1", "Tissue specific sequencing libraries were prepped and barcoded using the BiooScientific NEXTflex smallRNA Sequencing Kit  which uses a 3\u2019 adenylated adapter that ligates onto miRs and other small RNAs with a 3\u2019 hydroxyl group.", null, null, "miRNA-Seq", "TRANSCRIPTOMIC", "unspecified", "SINGLE", "ILLUMINA", "Illumina HiSeq 2500", "<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>51</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>", "SRP039502", null, null, "ZF1_Brain_2.fq.gz", "fastq", 838372782.0, 16438682.0, "Brain1 Run2", "0:51", "A:206906228;C:221202670;G:210716690;T:195319694;N:4227500", 51, null, null, null, 206906228, 221202670, 210716690, 195319694, 4227500, "SRX481992", "SRS566490", "SRA142461", "University of Oregon|JHP-Lab", "University of Oregon", 1, 0.0, null, 0.0, null, 1.0, null, null, null, 51, null, "T", null, "under 1.2% mapping rate", "illumina", "hiseq_era", "unknown", "small_rna", "unknown", "bulk", "unknown", "unknown", null, "United States", "2014-08-22", "Adult", "Adult", "Brain", "Nervous System"], [38014, "SRR1265742", "SRX529137", "SRS598834", "SRP041544", "PRJNA245824", "Deep sequencing of small RNA facilitates tissue and sex associated microRNA discovery in zebrafish", "GSE57169", "Transcriptome Analysis", "The role of microRNAs in gene regulation has been well established. The extent of miRNA regulation also increases with increasing genome complexity. Though the number of genes appear to be equal between human and zebrafish  substantially less microRNAs have been discovered in zebrafish compared to human Release 19. It appears that most of the miRNAs in zebrafish are yet to be discovered. We sequenced small RNAs from brain  gut  liver  ovary  testis  eye  heart and embryo of zebrafish.  In brain  gut and liver sequencing was done in male and female separately. Majority of the sequenced reads 16 62% mapped to known miRNAs  with the exception of ovary 5.7% and testis 7.8%. Using the miRNA discovery tool miRDeep2  we discovered novel miRNAs from the un annotated reads that ranged from 7.6 to 23.0%  with exceptions of ovary 51.4% and testis 55.2%. The prediction tool identified a total of 459 novel pre miRNAs. We compared expression of miRNAs between different tissues and between males and females to identify tissue associated and sex associated miRNAs respectively. These miRNAs could serve as putative biomarkers for these tissues. The brain and liver had highest number of tissue associated 22 and sex associated 34 miRNAs  respectively. This study comprehensively identifies tissue and sex associated miRNAs in zebrafish. Further  we have discovered 459 novel pre miRNAs 30% seed homology to human miRNA as  a genomic resource which can facilitate further investigations to understand miRNA mRNA gene regulatory networks in zebrafish which will have implications in understanding the function of human homologs. Overall design: Known miRNA profiling  novel miRNA discovery and identification of tissue associated and sex associated miRNAs from sRNA deep sequencing data of different tissues and embryo of zebrafish in triplicate was carried out using the Illumina HiSeq 2000 platform.", null, "pubmed:26574018", null, "Female Brain Replicate 3 sRNAseq", "GSM1376625", null, "source name:Female Brain|gender:female|tissue:Brain|genetic background:Wild type   Singapore strain", "Female Brain Replicate 3 sRNAseq", "Illumina Casava 1.8.2 software used for basecalling. The sequenced reads were first subjected to adapter removal through the cutadapt program Martin 2011. The trimmed reads were then collapsed to remove redundancy and to obtain a unique sequence fasta file through the mapper module of miRDeep2 package Friedl\u00e4nder et al. 2012. The unique reads fasta file was then put through an elimination pipeline module Vaz et al. 2010 comprising of a series of sequence similarity searches with the annotated databases. At each step the reads were matched to an annotated database with a maximum of two mismatches. The matched reads were removed and the unmatched ones were further matched to another annotated database  finally culminating into an un annotated pool of reads that served as a source of novel miRNAs and novel sRNAs. The known miRNA expression profile was generated by using the quantifier module of the miRDeep2 package that gives the read counts for the known miRNAs. The quantifier.pl command line used: perl quantifier.pl  p <zebrafish precursor miRNA fasta file>  m <zebrafish mature miRNA fasta file>  r <unique reads fasta file>  t Zebrafish The raw reads expression profile generated for all the replicates of the samples were subjected to Trimmed Mean of M values TMM normalisation using the Bioconductor package edgeR Robinson et al. 2010. Genome build: ZV9 Supplementary files format and content: 1. 'Danio rerio known miRNA Rel19 profile Raw.txt': Tab delimited text file that includes the raw counts for the known mature miRNA miRBase Release 19. Supplementary files format and content: 2. 'Danio rerio known miRNA Rel19 profile Normalised.txt': Tab delimited text file that includes the Trimmed Mean of M values TMM normalised counts for the known mature miRNA miRBase Release 19. One of the replicate of Heart MZH008 failed to cluster with the other two replicates on basis of its known miRNA expression profile and hence was not used for further analysis.", "Female Brain", "N/A", "Total RNA were extracted using mirVana\u2122 miRNA Isolation Kit AM1560  Life Technologies. Tissues were homogenised in 1.5 ml microfuge tube containing Lysis/Binding buffer provided in the  mirVana\u2122 miRNA Isolation using a hand held pestle. Total RNA containing small RNA were purified following the manufacturer protocol. Small RNA libraries were prepared for sequencing using  TruSeq Small RNA Sample Preparation Kit  RS 200 0012  Illumina  Inc.. Libraries were prepared according to manufacturer instructions. Briefly  1\u00b5g of good quality Total RNA per sample was used as starting material. 5\u2019 and 3\u2019 RNA adapters were ligated to each RNA molecule before reverse transcription to create single stranded cDNA. The cDNA was then amplified with PCR using a common primer and a primer containing a unique index sequence. The resulting PCR reactions were electrophoresed on 6% Novex TBE PAGE Gel Life Technologies and bands corresponding to adapter ligated constructs derived from 22 30 nucleotides small RNA fragments were excised from the gel. The small RNA were purified from the excised gel and validated on High Sensitivity DNA chips on a Bioanalyser Agilent Technologies before sequencing.", "Fishes were purchased from a local supplier and acclimatized before tissue extraction.", "gender:Female|tissue:Brain|genetic background:Wild type   Singapore strain", "GSM1376625", "GSM1376625: Female Brain Replicate 3 sRNAseq; Danio rerio; miRNA Seq", "GSM1376625", null, "1", "Total RNA were extracted using mirVana\u2122 miRNA Isolation Kit AM1560  Life Technologies. Tissues were homogenised in 1.5 ml microfuge tube containing Lysis/Binding buffer provided in the  mirVana\u2122 miRNA Isolation using a hand held pestle. Total RNA containing small RNA were purified following the manufacturer protocol. Small RNA libraries were prepared for sequencing using  TruSeq Small RNA Sample Preparation Kit  RS 200 0012  Illumina  Inc.. Libraries were prepared according to manufacturer instructions. Briefly  1\u00b5g of good quality Total RNA per sample was used as starting material. 5\u2019 and 3\u2019 RNA adapters were ligated to each RNA molecule before reverse transcription to create single stranded cDNA. The cDNA was then amplified with PCR using a common primer and a primer containing a unique index sequence. The resulting PCR reactions were electrophoresed on 6% Novex TBE PAGE Gel Life Technologies and bands corresponding to adapter ligated constructs derived from 22 30 nucleotides small RNA fragments were excised from the gel. The small RNA were purified from the excised gel and validated on High Sensitivity DNA chips on a Bioanalyser Agilent Technologies before sequencing.", "GEO Accession:GSM1376625", "miRNA-Seq", "TRANSCRIPTOMIC", "size fractionation", "SINGLE", "ILLUMINA", "Illumina HiSeq 2000", null, "SRP041544", null, null, "MZB012_CTTGTA_L007_R1.fastq.gz", "fastq", 657620928.0, 12894528.0, "GSM1376625 r1", "0:51", "A:127428594;C:157315630;G:203096060;T:169719399;N:61245", 51, null, null, null, 127428594, 157315630, 203096060, 169719399, 61245, "SRX529137", "SRS598834", "SRA160430", "GEO", "Expression and Signaling in Mesenchymal and Hematopoietic Stem Cells, Genome and Gene Expression Data Analysis Division, Bioinformatics Institute, A*STAR, Singapore", 1, 0.01165, null, 0.00109, null, 0.99622, null, 0.72322, null, 51, null, "T", null, "under 1.2% mapping rate", "illumina", "hiseq_era", "unknown", "size_fractionation", "trueseq", "bulk", "unknown", "unknown", null, "Singapore", "2014-04-29", "Undetermined", "Embryo", "Brain", "Nervous System"]], "truncated": false, "filtered_table_rows_count": 1247, "expanded_columns": [], "expandable_columns": [], "columns": ["rowid", "run.accession", "experiment.accession", "sample.accession", "study.accession", "bioproject", "study.title", "study.alias", "study.type", "study.abstract", "study.attributes", "study.PMIDs", "sample.description", "sample.title", "sample.alias", "sample.centername", "sample.attributes", "GEOsample.title", "GEOsample.dataprocessing", "GEOsample.source", "GEOsample.treatmentprotocol", "GEOsample.extractprotocol", "GEOsample.growthprotocol", "GEOsample.characteristics", "GEOsample.accession", "experiment.title", "experiment.alias", "experiment.library_name", "experiment.design_description", "experiment.library_construction_protocol", "experiment.attributes", "experiment.library_strategy", "experiment.library_source", "experiment.library_selection", "experiment.library_layout", "experiment.platform", 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