{"database": "metadata", "table": "run_metadata", "is_view": false, "human_description_en": "where experiment.library_layout = \"SINGLE\", technology = \"bulk\" and tissue_curation = \"Skin\"", "rows": [[36741, "SRR850608", "SRX277695", "SRS420758", "SRP022612", "PRJNA203029", "RNA seq of Danio rerio and Mus musculus skin for three different age groups", "GSE46916", "Other", "Background: Comparison of temporal gene expression profiles. The RNA seq data comprises 3 age groups: 2  15 month and 30 month for mouse skin; 5  24 month and 42 month for zebrafish skin. Illumina 50bp single stranded single read RNA sequencing Jena Centre for Systems Biology of Ageing   JenAge www.jenage.de Overall design: 15 samples for mouse: 5 biological replicates for 2 month  6 biological replicates for 15 month and 4 biological replicates for 30 month; 20 samples for zebrafish: 9 biological replicates for 5 month  6 biological replicates for 24 month and 5 biological replicates for 42 month", null, "pubmed:26620638", null, "Nr18 danrer skin 42months rep5", "GSM1141118", null, "source name:total RNA extracted from skin|strain:TuAB|age:42 month|tissue:skin", "Nr18 danrer skin 42months rep5", "Illumina Casava software used for extraction of FASTQ files FASTQ files were mapped using Bowtie vs. the respective genomic sequence and a splice site data set created using UCSC's RefSeq annotation for each species Counting of uniquely mapped reads and assignment to RefSeq transcripts/genes using R Statistical Language and Bioconductor Calculation of RPKM values for each transcript and gene from the corresponding RefSeq annotation Genome build: D.\u00a0rerio: danRer7; M. musculus: mm9 Supplementary files format and content: Excel file include raw counts and RPKM values for each sample", "total RNA extracted from skin", null, "D.\u00a0rerio: Skin tissue from male zebrafish was dissected and stored in RNAlater Qiagen  Hilden  Germany at 80 \u00b0C. Total RNA was isolated with TRIzol Life Technologies  Darmstadt  Germany according to the instructions of the manufacturer. M.\u00a0musculus: The isolated skin was homogenized in 500\u00a0\u03bcl QIAzol Qiagen by subsequently adding 100\u00a0\u03bcl chloroform. Following phase separation  the aqueous phase was transferred into a fresh tube  then 0.16 volume NaAc 2\u00a0M  pH\u00a04.0 and 1.1 volume isopropanol were added. The RNA was precipitated by centrifugation and the pellet was washed with 75% ethanol. Total RNA was resuspended in 20\u00a0\u03bcl water GIBCO and stored at \u201380\u00b0C until use. D.\u00a0rerio: 5\u00a0\u00b5g of total RNA was used for preparation of libraries using Illumina\u2019s mRNA Seq sample prep kit Illumina  San Diego  CA  USA following the manufacturer\u2019s instruction. M.\u00a0musculus: 2.5\u00a0\u00b5g of total RNA was used with Illumina\u2019s TruSeq RNA sample prep kit.", null, "strain:TuAB|age:42 month|tissue:skin", "GSM1141118", "GSM1141118: Nr18 danrer skin 42months rep5; Danio rerio; RNA Seq", "GSM1141118 1", null, "1", "D.\u00a0rerio: Skin tissue from male zebrafish was dissected and stored in RNAlater Qiagen  Hilden  Germany at 80 \u00b0C. Total RNA was isolated with TRIzol Life Technologies  Darmstadt  Germany according to the instructions of the manufacturer. M.\u00a0musculus: The isolated skin was homogenized in 500\u00a0\u03bcl QIAzol Qiagen by subsequently adding 100\u00a0\u03bcl chloroform. Following phase separation  the aqueous phase was transferred into a fresh tube  then 0.16 volume NaAc 2\u00a0M  pH\u00a04.0 and 1.1 volume isopropanol were added. The RNA was precipitated by centrifugation and the pellet was washed with 75% ethanol. Total RNA was resuspended in 20\u00a0\u03bcl water GIBCO and stored at \u201380\u00b0C until use. D.\u00a0rerio: 5\u00a0\u00b5g of total RNA was used for preparation of libraries using Illumina\u2019s mRNA Seq sample prep kit Illumina  San Diego  CA  USA following the manufacturer\u2019s instruction. M.\u00a0musculus: 2.5\u00a0\u00b5g of total RNA was used with Illumina\u2019s TruSeq RNA sample prep kit.", "GEO Accession:GSM1141118", "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "SINGLE", "ILLUMINA", "Illumina Genome Analyzer IIx", null, "SRP022612", null, null, "Nr18_3.5y_l3_export.fq.gz", "fastq", 3269418540.0, 43018665.0, "GSM1141118 r1", "0:76", "A:892486836;C:744426899;G:749230539;T:883114979;N:159287", 76, null, null, null, 892486836, 744426899, 749230539, 883114979, 159287, "SRX277695", "SRS420758", "SRA076297", "GEO", "Leibniz Institute for Age Research - Fritz Lipmann Institute", 1, 0.91402, null, 0.1106, null, 0.73436, null, 0.4911, null, 76, null, "B", null, "usable mapping rate", "illumina", "early_illumina", "unknown", "cdna_unspecified", "trueseq", "bulk", "bulk", "bulk", null, "Germany", "2013-05-14", "Adult", "Adult", "Skin", "Surface Structure"], [36742, "SRR850607", "SRX277694", "SRS420757", "SRP022612", "PRJNA203029", "RNA seq of Danio rerio and Mus musculus skin for three different age groups", "GSE46916", "Other", "Background: Comparison of temporal gene expression profiles. The RNA seq data comprises 3 age groups: 2  15 month and 30 month for mouse skin; 5  24 month and 42 month for zebrafish skin. Illumina 50bp single stranded single read RNA sequencing Jena Centre for Systems Biology of Ageing   JenAge www.jenage.de Overall design: 15 samples for mouse: 5 biological replicates for 2 month  6 biological replicates for 15 month and 4 biological replicates for 30 month; 20 samples for zebrafish: 9 biological replicates for 5 month  6 biological replicates for 24 month and 5 biological replicates for 42 month", null, "pubmed:26620638", null, "Nr14 danrer skin 42months rep4", "GSM1141117", null, "source name:total RNA extracted from skin|strain:TuAB|age:42 month|tissue:skin", "Nr14 danrer skin 42months rep4", "Illumina Casava software used for extraction of FASTQ files FASTQ files were mapped using Bowtie vs. the respective genomic sequence and a splice site data set created using UCSC's RefSeq annotation for each species Counting of uniquely mapped reads and assignment to RefSeq transcripts/genes using R Statistical Language and Bioconductor Calculation of RPKM values for each transcript and gene from the corresponding RefSeq annotation Genome build: D.\u00a0rerio: danRer7; M. musculus: mm9 Supplementary files format and content: Excel file include raw counts and RPKM values for each sample", "total RNA extracted from skin", null, "D.\u00a0rerio: Skin tissue from male zebrafish was dissected and stored in RNAlater Qiagen  Hilden  Germany at 80 \u00b0C. Total RNA was isolated with TRIzol Life Technologies  Darmstadt  Germany according to the instructions of the manufacturer. M.\u00a0musculus: The isolated skin was homogenized in 500\u00a0\u03bcl QIAzol Qiagen by subsequently adding 100\u00a0\u03bcl chloroform. Following phase separation  the aqueous phase was transferred into a fresh tube  then 0.16 volume NaAc 2\u00a0M  pH\u00a04.0 and 1.1 volume isopropanol were added. The RNA was precipitated by centrifugation and the pellet was washed with 75% ethanol. Total RNA was resuspended in 20\u00a0\u03bcl water GIBCO and stored at \u201380\u00b0C until use. D.\u00a0rerio: 5\u00a0\u00b5g of total RNA was used for preparation of libraries using Illumina\u2019s mRNA Seq sample prep kit Illumina  San Diego  CA  USA following the manufacturer\u2019s instruction. M.\u00a0musculus: 2.5\u00a0\u00b5g of total RNA was used with Illumina\u2019s TruSeq RNA sample prep kit.", null, "strain:TuAB|age:42 month|tissue:skin", "GSM1141117", "GSM1141117: Nr14 danrer skin 42months rep4; Danio rerio; RNA Seq", "GSM1141117 1", null, "1", "D.\u00a0rerio: Skin tissue from male zebrafish was dissected and stored in RNAlater Qiagen  Hilden  Germany at 80 \u00b0C. Total RNA was isolated with TRIzol Life Technologies  Darmstadt  Germany according to the instructions of the manufacturer. M.\u00a0musculus: The isolated skin was homogenized in 500\u00a0\u03bcl QIAzol Qiagen by subsequently adding 100\u00a0\u03bcl chloroform. Following phase separation  the aqueous phase was transferred into a fresh tube  then 0.16 volume NaAc 2\u00a0M  pH\u00a04.0 and 1.1 volume isopropanol were added. The RNA was precipitated by centrifugation and the pellet was washed with 75% ethanol. Total RNA was resuspended in 20\u00a0\u03bcl water GIBCO and stored at \u201380\u00b0C until use. D.\u00a0rerio: 5\u00a0\u00b5g of total RNA was used for preparation of libraries using Illumina\u2019s mRNA Seq sample prep kit Illumina  San Diego  CA  USA following the manufacturer\u2019s instruction. M.\u00a0musculus: 2.5\u00a0\u00b5g of total RNA was used with Illumina\u2019s TruSeq RNA sample prep kit.", "GEO Accession:GSM1141117", "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "SINGLE", "ILLUMINA", "Illumina Genome Analyzer IIx", null, "SRP022612", null, null, "Nr14_3.5y_l2_export.fq.gz", "fastq", 2300890044.0, 30274869.0, "GSM1141117 r1", "0:76", "A:631736236;C:525065199;G:510761115;T:633208038;N:119456", 76, null, null, null, 631736236, 525065199, 510761115, 633208038, 119456, "SRX277694", "SRS420757", "SRA076297", "GEO", "Leibniz Institute for Age Research - Fritz Lipmann Institute", 1, 0.90718, null, 0.11607, null, 0.71997, null, 0.49141, null, 76, null, "B", null, "usable mapping rate", "illumina", "early_illumina", "unknown", "cdna_unspecified", "trueseq", "bulk", "bulk", "bulk", null, "Germany", "2013-05-14", "Adult", "Adult", "Skin", "Surface Structure"], [36743, "SRR850606", "SRX277693", "SRS420790", "SRP022612", "PRJNA203029", "RNA seq of Danio rerio and Mus musculus skin for three different age groups", "GSE46916", "Other", "Background: Comparison of temporal gene expression profiles. The RNA seq data comprises 3 age groups: 2  15 month and 30 month for mouse skin; 5  24 month and 42 month for zebrafish skin. Illumina 50bp single stranded single read RNA sequencing Jena Centre for Systems Biology of Ageing   JenAge www.jenage.de Overall design: 15 samples for mouse: 5 biological replicates for 2 month  6 biological replicates for 15 month and 4 biological replicates for 30 month; 20 samples for zebrafish: 9 biological replicates for 5 month  6 biological replicates for 24 month and 5 biological replicates for 42 month", null, "pubmed:26620638", null, "Nr13 danrer skin 42months rep3", "GSM1141116", null, "source name:total RNA extracted from skin|strain:TuAB|age:42 month|tissue:skin", "Nr13 danrer skin 42months rep3", "Illumina Casava software used for extraction of FASTQ files FASTQ files were mapped using Bowtie vs. the respective genomic sequence and a splice site data set created using UCSC's RefSeq annotation for each species Counting of uniquely mapped reads and assignment to RefSeq transcripts/genes using R Statistical Language and Bioconductor Calculation of RPKM values for each transcript and gene from the corresponding RefSeq annotation Genome build: D.\u00a0rerio: danRer7; M. musculus: mm9 Supplementary files format and content: Excel file include raw counts and RPKM values for each sample", "total RNA extracted from skin", null, "D.\u00a0rerio: Skin tissue from male zebrafish was dissected and stored in RNAlater Qiagen  Hilden  Germany at 80 \u00b0C. Total RNA was isolated with TRIzol Life Technologies  Darmstadt  Germany according to the instructions of the manufacturer. M.\u00a0musculus: The isolated skin was homogenized in 500\u00a0\u03bcl QIAzol Qiagen by subsequently adding 100\u00a0\u03bcl chloroform. Following phase separation  the aqueous phase was transferred into a fresh tube  then 0.16 volume NaAc 2\u00a0M  pH\u00a04.0 and 1.1 volume isopropanol were added. The RNA was precipitated by centrifugation and the pellet was washed with 75% ethanol. Total RNA was resuspended in 20\u00a0\u03bcl water GIBCO and stored at \u201380\u00b0C until use. D.\u00a0rerio: 5\u00a0\u00b5g of total RNA was used for preparation of libraries using Illumina\u2019s mRNA Seq sample prep kit Illumina  San Diego  CA  USA following the manufacturer\u2019s instruction. M.\u00a0musculus: 2.5\u00a0\u00b5g of total RNA was used with Illumina\u2019s TruSeq RNA sample prep kit.", null, "strain:TuAB|age:42 month|tissue:skin", "GSM1141116", "GSM1141116: Nr13 danrer skin 42months rep3; Danio rerio; RNA Seq", "GSM1141116 1", null, "1", "D.\u00a0rerio: Skin tissue from male zebrafish was dissected and stored in RNAlater Qiagen  Hilden  Germany at 80 \u00b0C. Total RNA was isolated with TRIzol Life Technologies  Darmstadt  Germany according to the instructions of the manufacturer. M.\u00a0musculus: The isolated skin was homogenized in 500\u00a0\u03bcl QIAzol Qiagen by subsequently adding 100\u00a0\u03bcl chloroform. Following phase separation  the aqueous phase was transferred into a fresh tube  then 0.16 volume NaAc 2\u00a0M  pH\u00a04.0 and 1.1 volume isopropanol were added. The RNA was precipitated by centrifugation and the pellet was washed with 75% ethanol. Total RNA was resuspended in 20\u00a0\u03bcl water GIBCO and stored at \u201380\u00b0C until use. D.\u00a0rerio: 5\u00a0\u00b5g of total RNA was used for preparation of libraries using Illumina\u2019s mRNA Seq sample prep kit Illumina  San Diego  CA  USA following the manufacturer\u2019s instruction. M.\u00a0musculus: 2.5\u00a0\u00b5g of total RNA was used with Illumina\u2019s TruSeq RNA sample prep kit.", "GEO Accession:GSM1141116", "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "SINGLE", "ILLUMINA", "Illumina Genome Analyzer IIx", null, "SRP022612", null, null, "Nr13_3.5y_l1_export.fastq.gz", "fastq", 3508110372.0, 46159347.0, "GSM1141116 r1", "0:76", "A:959986155;C:782258637;G:803256847;T:962450623;N:158110", 76, null, null, null, 959986155, 782258637, 803256847, 962450623, 158110, "SRX277693", "SRS420790", "SRA076297", "GEO", "Leibniz Institute for Age Research - Fritz Lipmann Institute", 1, 0.85747, null, 0.12068, null, 0.73819, null, 0.4917, null, 76, null, "B", null, "usable mapping rate", "illumina", "early_illumina", "unknown", "cdna_unspecified", "trueseq", "bulk", "bulk", "bulk", null, "Germany", "2013-05-14", "Adult", "Adult", "Skin", "Surface Structure"], [36744, "SRR850605", "SRX277692", "SRS420789", "SRP022612", "PRJNA203029", "RNA seq of Danio rerio and Mus musculus skin for three different age groups", "GSE46916", "Other", "Background: Comparison of temporal gene expression profiles. The RNA seq data comprises 3 age groups: 2  15 month and 30 month for mouse skin; 5  24 month and 42 month for zebrafish skin. Illumina 50bp single stranded single read RNA sequencing Jena Centre for Systems Biology of Ageing   JenAge www.jenage.de Overall design: 15 samples for mouse: 5 biological replicates for 2 month  6 biological replicates for 15 month and 4 biological replicates for 30 month; 20 samples for zebrafish: 9 biological replicates for 5 month  6 biological replicates for 24 month and 5 biological replicates for 42 month", null, "pubmed:26620638", null, "Nr12 danrer skin 42months rep2", "GSM1141115", null, "source name:total RNA extracted from skin|strain:TuAB|age:42 month|tissue:skin", "Nr12 danrer skin 42months rep2", "Illumina Casava software used for extraction of FASTQ files FASTQ files were mapped using Bowtie vs. the respective genomic sequence and a splice site data set created using UCSC's RefSeq annotation for each species Counting of uniquely mapped reads and assignment to RefSeq transcripts/genes using R Statistical Language and Bioconductor Calculation of RPKM values for each transcript and gene from the corresponding RefSeq annotation Genome build: D.\u00a0rerio: danRer7; M. musculus: mm9 Supplementary files format and content: Excel file include raw counts and RPKM values for each sample", "total RNA extracted from skin", null, "D.\u00a0rerio: Skin tissue from male zebrafish was dissected and stored in RNAlater Qiagen  Hilden  Germany at 80 \u00b0C. Total RNA was isolated with TRIzol Life Technologies  Darmstadt  Germany according to the instructions of the manufacturer. M.\u00a0musculus: The isolated skin was homogenized in 500\u00a0\u03bcl QIAzol Qiagen by subsequently adding 100\u00a0\u03bcl chloroform. Following phase separation  the aqueous phase was transferred into a fresh tube  then 0.16 volume NaAc 2\u00a0M  pH\u00a04.0 and 1.1 volume isopropanol were added. The RNA was precipitated by centrifugation and the pellet was washed with 75% ethanol. Total RNA was resuspended in 20\u00a0\u03bcl water GIBCO and stored at \u201380\u00b0C until use. D.\u00a0rerio: 5\u00a0\u00b5g of total RNA was used for preparation of libraries using Illumina\u2019s mRNA Seq sample prep kit Illumina  San Diego  CA  USA following the manufacturer\u2019s instruction. M.\u00a0musculus: 2.5\u00a0\u00b5g of total RNA was used with Illumina\u2019s TruSeq RNA sample prep kit.", null, "strain:TuAB|age:42 month|tissue:skin", "GSM1141115", "GSM1141115: Nr12 danrer skin 42months rep2; Danio rerio; RNA Seq", "GSM1141115 1", null, "1", "D.\u00a0rerio: Skin tissue from male zebrafish was dissected and stored in RNAlater Qiagen  Hilden  Germany at 80 \u00b0C. Total RNA was isolated with TRIzol Life Technologies  Darmstadt  Germany according to the instructions of the manufacturer. M.\u00a0musculus: The isolated skin was homogenized in 500\u00a0\u03bcl QIAzol Qiagen by subsequently adding 100\u00a0\u03bcl chloroform. Following phase separation  the aqueous phase was transferred into a fresh tube  then 0.16 volume NaAc 2\u00a0M  pH\u00a04.0 and 1.1 volume isopropanol were added. The RNA was precipitated by centrifugation and the pellet was washed with 75% ethanol. Total RNA was resuspended in 20\u00a0\u03bcl water GIBCO and stored at \u201380\u00b0C until use. D.\u00a0rerio: 5\u00a0\u00b5g of total RNA was used for preparation of libraries using Illumina\u2019s mRNA Seq sample prep kit Illumina  San Diego  CA  USA following the manufacturer\u2019s instruction. M.\u00a0musculus: 2.5\u00a0\u00b5g of total RNA was used with Illumina\u2019s TruSeq RNA sample prep kit.", "GEO Accession:GSM1141115", "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "SINGLE", "ILLUMINA", "Illumina Genome Analyzer IIx", null, "SRP022612", null, null, "Nr12_3.5y_l3_export.fq.gz", "fastq", 3961243804.0, 52121629.0, "GSM1141115 r1", "0:76", "A:1092811778;C:874440215;G:892629531;T:1099367104;N:1995176", 76, null, null, null, 1092811778, 874440215, 892629531, 1099367104, 1995176, "SRX277692", "SRS420789", "SRA076297", "GEO", "Leibniz Institute for Age Research - Fritz Lipmann Institute", 1, 0.88854, null, 0.12633, null, 0.73746, null, 0.53849, null, 76, null, "B", null, "usable mapping rate", "illumina", "early_illumina", "unknown", "cdna_unspecified", "trueseq", "bulk", "bulk", "bulk", null, "Germany", "2013-05-14", "Adult", "Adult", "Skin", "Surface Structure"], [36745, "SRR850604", "SRX277691", "SRS420788", "SRP022612", "PRJNA203029", "RNA seq of Danio rerio and Mus musculus skin for three different age groups", "GSE46916", "Other", "Background: Comparison of temporal gene expression profiles. The RNA seq data comprises 3 age groups: 2  15 month and 30 month for mouse skin; 5  24 month and 42 month for zebrafish skin. Illumina 50bp single stranded single read RNA sequencing Jena Centre for Systems Biology of Ageing   JenAge www.jenage.de Overall design: 15 samples for mouse: 5 biological replicates for 2 month  6 biological replicates for 15 month and 4 biological replicates for 30 month; 20 samples for zebrafish: 9 biological replicates for 5 month  6 biological replicates for 24 month and 5 biological replicates for 42 month", null, "pubmed:26620638", null, "Nr11 danrer skin 42months rep1", "GSM1141114", null, "source name:total RNA extracted from skin|strain:TuAB|age:42 month|tissue:skin", "Nr11 danrer skin 42months rep1", "Illumina Casava software used for extraction of FASTQ files FASTQ files were mapped using Bowtie vs. the respective genomic sequence and a splice site data set created using UCSC's RefSeq annotation for each species Counting of uniquely mapped reads and assignment to RefSeq transcripts/genes using R Statistical Language and Bioconductor Calculation of RPKM values for each transcript and gene from the corresponding RefSeq annotation Genome build: D.\u00a0rerio: danRer7; M. musculus: mm9 Supplementary files format and content: Excel file include raw counts and RPKM values for each sample", "total RNA extracted from skin", null, "D.\u00a0rerio: Skin tissue from male zebrafish was dissected and stored in RNAlater Qiagen  Hilden  Germany at 80 \u00b0C. Total RNA was isolated with TRIzol Life Technologies  Darmstadt  Germany according to the instructions of the manufacturer. M.\u00a0musculus: The isolated skin was homogenized in 500\u00a0\u03bcl QIAzol Qiagen by subsequently adding 100\u00a0\u03bcl chloroform. Following phase separation  the aqueous phase was transferred into a fresh tube  then 0.16 volume NaAc 2\u00a0M  pH\u00a04.0 and 1.1 volume isopropanol were added. The RNA was precipitated by centrifugation and the pellet was washed with 75% ethanol. Total RNA was resuspended in 20\u00a0\u03bcl water GIBCO and stored at \u201380\u00b0C until use. D.\u00a0rerio: 5\u00a0\u00b5g of total RNA was used for preparation of libraries using Illumina\u2019s mRNA Seq sample prep kit Illumina  San Diego  CA  USA following the manufacturer\u2019s instruction. M.\u00a0musculus: 2.5\u00a0\u00b5g of total RNA was used with Illumina\u2019s TruSeq RNA sample prep kit.", null, "strain:TuAB|age:42 month|tissue:skin", "GSM1141114", "GSM1141114: Nr11 danrer skin 42months rep1; Danio rerio; RNA Seq", "GSM1141114 1", null, "1", "D.\u00a0rerio: Skin tissue from male zebrafish was dissected and stored in RNAlater Qiagen  Hilden  Germany at 80 \u00b0C. Total RNA was isolated with TRIzol Life Technologies  Darmstadt  Germany according to the instructions of the manufacturer. M.\u00a0musculus: The isolated skin was homogenized in 500\u00a0\u03bcl QIAzol Qiagen by subsequently adding 100\u00a0\u03bcl chloroform. Following phase separation  the aqueous phase was transferred into a fresh tube  then 0.16 volume NaAc 2\u00a0M  pH\u00a04.0 and 1.1 volume isopropanol were added. The RNA was precipitated by centrifugation and the pellet was washed with 75% ethanol. Total RNA was resuspended in 20\u00a0\u03bcl water GIBCO and stored at \u201380\u00b0C until use. D.\u00a0rerio: 5\u00a0\u00b5g of total RNA was used for preparation of libraries using Illumina\u2019s mRNA Seq sample prep kit Illumina  San Diego  CA  USA following the manufacturer\u2019s instruction. M.\u00a0musculus: 2.5\u00a0\u00b5g of total RNA was used with Illumina\u2019s TruSeq RNA sample prep kit.", "GEO Accession:GSM1141114", "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "SINGLE", "ILLUMINA", "Illumina Genome Analyzer IIx", null, "SRP022612", null, null, "Nr11_3.5y_l2_export.fq.gz", "fastq", 3327777040.0, 43786540.0, "GSM1141114 r1", "0:76", "A:920364748;C:745739060;G:758842746;T:901558784;N:1271702", 76, null, null, null, 920364748, 745739060, 758842746, 901558784, 1271702, "SRX277691", "SRS420788", "SRA076297", "GEO", "Leibniz Institute for Age Research - Fritz Lipmann Institute", 1, 0.86781, null, 0.11618, null, 0.72888, null, 0.50217, null, 76, null, "B", null, "usable mapping rate", "illumina", "early_illumina", "unknown", "cdna_unspecified", "trueseq", "bulk", "bulk", "bulk", null, "Germany", "2013-05-14", "Adult", "Adult", "Skin", "Surface Structure"], [36746, "SRR850603", "SRX277690", "SRS420756", "SRP022612", "PRJNA203029", "RNA seq of Danio rerio and Mus musculus skin for three different age groups", "GSE46916", "Other", "Background: Comparison of temporal gene expression profiles. The RNA seq data comprises 3 age groups: 2  15 month and 30 month for mouse skin; 5  24 month and 42 month for zebrafish skin. Illumina 50bp single stranded single read RNA sequencing Jena Centre for Systems Biology of Ageing   JenAge www.jenage.de Overall design: 15 samples for mouse: 5 biological replicates for 2 month  6 biological replicates for 15 month and 4 biological replicates for 30 month; 20 samples for zebrafish: 9 biological replicates for 5 month  6 biological replicates for 24 month and 5 biological replicates for 42 month", null, "pubmed:26620638", null, "Nr76 danrer skin 24months rep6", "GSM1141113", null, "source name:total RNA extracted from skin|strain:TuAB|age:24 month|tissue:skin", "Nr76 danrer skin 24months rep6", "Illumina Casava software used for extraction of FASTQ files FASTQ files were mapped using Bowtie vs. the respective genomic sequence and a splice site data set created using UCSC's RefSeq annotation for each species Counting of uniquely mapped reads and assignment to RefSeq transcripts/genes using R Statistical Language and Bioconductor Calculation of RPKM values for each transcript and gene from the corresponding RefSeq annotation Genome build: D.\u00a0rerio: danRer7; M. musculus: mm9 Supplementary files format and content: Excel file include raw counts and RPKM values for each sample", "total RNA extracted from skin", null, "D.\u00a0rerio: Skin tissue from male zebrafish was dissected and stored in RNAlater Qiagen  Hilden  Germany at 80 \u00b0C. Total RNA was isolated with TRIzol Life Technologies  Darmstadt  Germany according to the instructions of the manufacturer. M.\u00a0musculus: The isolated skin was homogenized in 500\u00a0\u03bcl QIAzol Qiagen by subsequently adding 100\u00a0\u03bcl chloroform. Following phase separation  the aqueous phase was transferred into a fresh tube  then 0.16 volume NaAc 2\u00a0M  pH\u00a04.0 and 1.1 volume isopropanol were added. The RNA was precipitated by centrifugation and the pellet was washed with 75% ethanol. Total RNA was resuspended in 20\u00a0\u03bcl water GIBCO and stored at \u201380\u00b0C until use. D.\u00a0rerio: 5\u00a0\u00b5g of total RNA was used for preparation of libraries using Illumina\u2019s mRNA Seq sample prep kit Illumina  San Diego  CA  USA following the manufacturer\u2019s instruction. M.\u00a0musculus: 2.5\u00a0\u00b5g of total RNA was used with Illumina\u2019s TruSeq RNA sample prep kit.", null, "strain:TuAB|age:24 month|tissue:skin", "GSM1141113", "GSM1141113: Nr76 danrer skin 24months rep6; Danio rerio; RNA Seq", "GSM1141113 1", null, "1", "D.\u00a0rerio: Skin tissue from male zebrafish was dissected and stored in RNAlater Qiagen  Hilden  Germany at 80 \u00b0C. Total RNA was isolated with TRIzol Life Technologies  Darmstadt  Germany according to the instructions of the manufacturer. M.\u00a0musculus: The isolated skin was homogenized in 500\u00a0\u03bcl QIAzol Qiagen by subsequently adding 100\u00a0\u03bcl chloroform. Following phase separation  the aqueous phase was transferred into a fresh tube  then 0.16 volume NaAc 2\u00a0M  pH\u00a04.0 and 1.1 volume isopropanol were added. The RNA was precipitated by centrifugation and the pellet was washed with 75% ethanol. Total RNA was resuspended in 20\u00a0\u03bcl water GIBCO and stored at \u201380\u00b0C until use. D.\u00a0rerio: 5\u00a0\u00b5g of total RNA was used for preparation of libraries using Illumina\u2019s mRNA Seq sample prep kit Illumina  San Diego  CA  USA following the manufacturer\u2019s instruction. M.\u00a0musculus: 2.5\u00a0\u00b5g of total RNA was used with Illumina\u2019s TruSeq RNA sample prep kit.", "GEO Accession:GSM1141113", "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "SINGLE", "ILLUMINA", "Illumina Genome Analyzer IIx", null, "SRP022612", null, null, "Nr76_2y.fastq.gz", "fastq", 2234765940.0, 29404815.0, "GSM1141113 r1", "0:76", "A:582794172;C:524083367;G:547601836;T:579473821;N:812744", 76, null, null, null, 582794172, 524083367, 547601836, 579473821, 812744, "SRX277690", "SRS420756", "SRA076297", "GEO", "Leibniz Institute for Age Research - Fritz Lipmann Institute", 1, 0.89808, null, 0.07989, null, 0.73511, null, 0.47456, null, 76, null, "B", null, "usable mapping rate", "illumina", "early_illumina", "unknown", "cdna_unspecified", "trueseq", "bulk", "bulk", "bulk", null, "Germany", "2013-05-14", "Adult", "Adult", "Skin", "Surface Structure"], [36747, "SRR850602", "SRX277689", "SRS420755", "SRP022612", "PRJNA203029", "RNA seq of Danio rerio and Mus musculus skin for three different age groups", "GSE46916", "Other", "Background: Comparison of temporal gene expression profiles. The RNA seq data comprises 3 age groups: 2  15 month and 30 month for mouse skin; 5  24 month and 42 month for zebrafish skin. Illumina 50bp single stranded single read RNA sequencing Jena Centre for Systems Biology of Ageing   JenAge www.jenage.de Overall design: 15 samples for mouse: 5 biological replicates for 2 month  6 biological replicates for 15 month and 4 biological replicates for 30 month; 20 samples for zebrafish: 9 biological replicates for 5 month  6 biological replicates for 24 month and 5 biological replicates for 42 month", null, "pubmed:26620638", null, "Nr75 danrer skin 24months rep5", "GSM1141112", null, "source name:total RNA extracted from skin|strain:TuAB|age:24 month|tissue:skin", "Nr75 danrer skin 24months rep5", "Illumina Casava software used for extraction of FASTQ files FASTQ files were mapped using Bowtie vs. the respective genomic sequence and a splice site data set created using UCSC's RefSeq annotation for each species Counting of uniquely mapped reads and assignment to RefSeq transcripts/genes using R Statistical Language and Bioconductor Calculation of RPKM values for each transcript and gene from the corresponding RefSeq annotation Genome build: D.\u00a0rerio: danRer7; M. musculus: mm9 Supplementary files format and content: Excel file include raw counts and RPKM values for each sample", "total RNA extracted from skin", null, "D.\u00a0rerio: Skin tissue from male zebrafish was dissected and stored in RNAlater Qiagen  Hilden  Germany at 80 \u00b0C. Total RNA was isolated with TRIzol Life Technologies  Darmstadt  Germany according to the instructions of the manufacturer. M.\u00a0musculus: The isolated skin was homogenized in 500\u00a0\u03bcl QIAzol Qiagen by subsequently adding 100\u00a0\u03bcl chloroform. Following phase separation  the aqueous phase was transferred into a fresh tube  then 0.16 volume NaAc 2\u00a0M  pH\u00a04.0 and 1.1 volume isopropanol were added. The RNA was precipitated by centrifugation and the pellet was washed with 75% ethanol. Total RNA was resuspended in 20\u00a0\u03bcl water GIBCO and stored at \u201380\u00b0C until use. D.\u00a0rerio: 5\u00a0\u00b5g of total RNA was used for preparation of libraries using Illumina\u2019s mRNA Seq sample prep kit Illumina  San Diego  CA  USA following the manufacturer\u2019s instruction. M.\u00a0musculus: 2.5\u00a0\u00b5g of total RNA was used with Illumina\u2019s TruSeq RNA sample prep kit.", null, "strain:TuAB|age:24 month|tissue:skin", "GSM1141112", "GSM1141112: Nr75 danrer skin 24months rep5; Danio rerio; RNA Seq", "GSM1141112 1", null, "1", "D.\u00a0rerio: Skin tissue from male zebrafish was dissected and stored in RNAlater Qiagen  Hilden  Germany at 80 \u00b0C. Total RNA was isolated with TRIzol Life Technologies  Darmstadt  Germany according to the instructions of the manufacturer. M.\u00a0musculus: The isolated skin was homogenized in 500\u00a0\u03bcl QIAzol Qiagen by subsequently adding 100\u00a0\u03bcl chloroform. Following phase separation  the aqueous phase was transferred into a fresh tube  then 0.16 volume NaAc 2\u00a0M  pH\u00a04.0 and 1.1 volume isopropanol were added. The RNA was precipitated by centrifugation and the pellet was washed with 75% ethanol. Total RNA was resuspended in 20\u00a0\u03bcl water GIBCO and stored at \u201380\u00b0C until use. D.\u00a0rerio: 5\u00a0\u00b5g of total RNA was used for preparation of libraries using Illumina\u2019s mRNA Seq sample prep kit Illumina  San Diego  CA  USA following the manufacturer\u2019s instruction. M.\u00a0musculus: 2.5\u00a0\u00b5g of total RNA was used with Illumina\u2019s TruSeq RNA sample prep kit.", "GEO Accession:GSM1141112", "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "SINGLE", "ILLUMINA", "Illumina Genome Analyzer IIx", null, "SRP022612", null, null, "Nr75_2y.fastq.gz", "fastq", 1000606196.0, 13165871.0, "GSM1141112 r1", "0:76", "A:270569436;C:233353660;G:238144829;T:258045669;N:492602", 76, null, null, null, 270569436, 233353660, 238144829, 258045669, 492602, "SRX277689", "SRS420755", "SRA076297", "GEO", "Leibniz Institute for Age Research - Fritz Lipmann Institute", 1, 0.8949, null, 0.07966, null, 0.7293, null, 0.47729, null, 76, null, "B", null, "usable mapping rate", "illumina", "early_illumina", "unknown", "cdna_unspecified", "trueseq", "bulk", "bulk", "bulk", null, "Germany", "2013-05-14", "Adult", "Adult", "Skin", "Surface Structure"], [36748, "SRR850601", "SRX277688", "SRS420754", "SRP022612", "PRJNA203029", "RNA seq of Danio rerio and Mus musculus skin for three different age groups", "GSE46916", "Other", "Background: Comparison of temporal gene expression profiles. The RNA seq data comprises 3 age groups: 2  15 month and 30 month for mouse skin; 5  24 month and 42 month for zebrafish skin. Illumina 50bp single stranded single read RNA sequencing Jena Centre for Systems Biology of Ageing   JenAge www.jenage.de Overall design: 15 samples for mouse: 5 biological replicates for 2 month  6 biological replicates for 15 month and 4 biological replicates for 30 month; 20 samples for zebrafish: 9 biological replicates for 5 month  6 biological replicates for 24 month and 5 biological replicates for 42 month", null, "pubmed:26620638", null, "Nr73 danrer skin 24months rep4", "GSM1141111", null, "source name:total RNA extracted from skin|strain:TuAB|age:24 month|tissue:skin", "Nr73 danrer skin 24months rep4", "Illumina Casava software used for extraction of FASTQ files FASTQ files were mapped using Bowtie vs. the respective genomic sequence and a splice site data set created using UCSC's RefSeq annotation for each species Counting of uniquely mapped reads and assignment to RefSeq transcripts/genes using R Statistical Language and Bioconductor Calculation of RPKM values for each transcript and gene from the corresponding RefSeq annotation Genome build: D.\u00a0rerio: danRer7; M. musculus: mm9 Supplementary files format and content: Excel file include raw counts and RPKM values for each sample", "total RNA extracted from skin", null, "D.\u00a0rerio: Skin tissue from male zebrafish was dissected and stored in RNAlater Qiagen  Hilden  Germany at 80 \u00b0C. Total RNA was isolated with TRIzol Life Technologies  Darmstadt  Germany according to the instructions of the manufacturer. M.\u00a0musculus: The isolated skin was homogenized in 500\u00a0\u03bcl QIAzol Qiagen by subsequently adding 100\u00a0\u03bcl chloroform. Following phase separation  the aqueous phase was transferred into a fresh tube  then 0.16 volume NaAc 2\u00a0M  pH\u00a04.0 and 1.1 volume isopropanol were added. The RNA was precipitated by centrifugation and the pellet was washed with 75% ethanol. Total RNA was resuspended in 20\u00a0\u03bcl water GIBCO and stored at \u201380\u00b0C until use. D.\u00a0rerio: 5\u00a0\u00b5g of total RNA was used for preparation of libraries using Illumina\u2019s mRNA Seq sample prep kit Illumina  San Diego  CA  USA following the manufacturer\u2019s instruction. M.\u00a0musculus: 2.5\u00a0\u00b5g of total RNA was used with Illumina\u2019s TruSeq RNA sample prep kit.", null, "strain:TuAB|age:24 month|tissue:skin", "GSM1141111", "GSM1141111: Nr73 danrer skin 24months rep4; Danio rerio; RNA Seq", "GSM1141111 1", null, "1", "D.\u00a0rerio: Skin tissue from male zebrafish was dissected and stored in RNAlater Qiagen  Hilden  Germany at 80 \u00b0C. Total RNA was isolated with TRIzol Life Technologies  Darmstadt  Germany according to the instructions of the manufacturer. M.\u00a0musculus: The isolated skin was homogenized in 500\u00a0\u03bcl QIAzol Qiagen by subsequently adding 100\u00a0\u03bcl chloroform. Following phase separation  the aqueous phase was transferred into a fresh tube  then 0.16 volume NaAc 2\u00a0M  pH\u00a04.0 and 1.1 volume isopropanol were added. The RNA was precipitated by centrifugation and the pellet was washed with 75% ethanol. Total RNA was resuspended in 20\u00a0\u03bcl water GIBCO and stored at \u201380\u00b0C until use. D.\u00a0rerio: 5\u00a0\u00b5g of total RNA was used for preparation of libraries using Illumina\u2019s mRNA Seq sample prep kit Illumina  San Diego  CA  USA following the manufacturer\u2019s instruction. M.\u00a0musculus: 2.5\u00a0\u00b5g of total RNA was used with Illumina\u2019s TruSeq RNA sample prep kit.", "GEO Accession:GSM1141111", "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "SINGLE", "ILLUMINA", "Illumina Genome Analyzer IIx", null, "SRP022612", null, null, "Nr73_2y.fastq.gz", "fastq", 2283103688.0, 30040838.0, "GSM1141111 r1", "0:76", "A:601138588;C:527906584;G:549863593;T:603391353;N:803570", 76, null, null, null, 601138588, 527906584, 549863593, 603391353, 803570, "SRX277688", "SRS420754", "SRA076297", "GEO", "Leibniz Institute for Age Research - Fritz Lipmann Institute", 1, 0.90138, null, 0.08154, null, 0.72598, null, 0.49455, null, 76, null, "B", null, "usable mapping rate", "illumina", "early_illumina", "unknown", "cdna_unspecified", "trueseq", "bulk", "bulk", "bulk", null, "Germany", "2013-05-14", "Adult", "Adult", "Skin", "Surface Structure"], [36749, "SRR850600", "SRX277687", "SRS420787", "SRP022612", "PRJNA203029", "RNA seq of Danio rerio and Mus musculus skin for three different age groups", "GSE46916", "Other", "Background: Comparison of temporal gene expression profiles. The RNA seq data comprises 3 age groups: 2  15 month and 30 month for mouse skin; 5  24 month and 42 month for zebrafish skin. Illumina 50bp single stranded single read RNA sequencing Jena Centre for Systems Biology of Ageing   JenAge www.jenage.de Overall design: 15 samples for mouse: 5 biological replicates for 2 month  6 biological replicates for 15 month and 4 biological replicates for 30 month; 20 samples for zebrafish: 9 biological replicates for 5 month  6 biological replicates for 24 month and 5 biological replicates for 42 month", null, "pubmed:26620638", null, "Nr72 danrer skin 24months rep3", "GSM1141110", null, "source name:total RNA extracted from skin|strain:TuAB|age:24 month|tissue:skin", "Nr72 danrer skin 24months rep3", "Illumina Casava software used for extraction of FASTQ files FASTQ files were mapped using Bowtie vs. the respective genomic sequence and a splice site data set created using UCSC's RefSeq annotation for each species Counting of uniquely mapped reads and assignment to RefSeq transcripts/genes using R Statistical Language and Bioconductor Calculation of RPKM values for each transcript and gene from the corresponding RefSeq annotation Genome build: D.\u00a0rerio: danRer7; M. musculus: mm9 Supplementary files format and content: Excel file include raw counts and RPKM values for each sample", "total RNA extracted from skin", null, "D.\u00a0rerio: Skin tissue from male zebrafish was dissected and stored in RNAlater Qiagen  Hilden  Germany at 80 \u00b0C. Total RNA was isolated with TRIzol Life Technologies  Darmstadt  Germany according to the instructions of the manufacturer. M.\u00a0musculus: The isolated skin was homogenized in 500\u00a0\u03bcl QIAzol Qiagen by subsequently adding 100\u00a0\u03bcl chloroform. Following phase separation  the aqueous phase was transferred into a fresh tube  then 0.16 volume NaAc 2\u00a0M  pH\u00a04.0 and 1.1 volume isopropanol were added. The RNA was precipitated by centrifugation and the pellet was washed with 75% ethanol. Total RNA was resuspended in 20\u00a0\u03bcl water GIBCO and stored at \u201380\u00b0C until use. D.\u00a0rerio: 5\u00a0\u00b5g of total RNA was used for preparation of libraries using Illumina\u2019s mRNA Seq sample prep kit Illumina  San Diego  CA  USA following the manufacturer\u2019s instruction. M.\u00a0musculus: 2.5\u00a0\u00b5g of total RNA was used with Illumina\u2019s TruSeq RNA sample prep kit.", null, "strain:TuAB|age:24 month|tissue:skin", "GSM1141110", "GSM1141110: Nr72 danrer skin 24months rep3; Danio rerio; RNA Seq", "GSM1141110 1", null, "1", "D.\u00a0rerio: Skin tissue from male zebrafish was dissected and stored in RNAlater Qiagen  Hilden  Germany at 80 \u00b0C. Total RNA was isolated with TRIzol Life Technologies  Darmstadt  Germany according to the instructions of the manufacturer. M.\u00a0musculus: The isolated skin was homogenized in 500\u00a0\u03bcl QIAzol Qiagen by subsequently adding 100\u00a0\u03bcl chloroform. Following phase separation  the aqueous phase was transferred into a fresh tube  then 0.16 volume NaAc 2\u00a0M  pH\u00a04.0 and 1.1 volume isopropanol were added. The RNA was precipitated by centrifugation and the pellet was washed with 75% ethanol. Total RNA was resuspended in 20\u00a0\u03bcl water GIBCO and stored at \u201380\u00b0C until use. D.\u00a0rerio: 5\u00a0\u00b5g of total RNA was used for preparation of libraries using Illumina\u2019s mRNA Seq sample prep kit Illumina  San Diego  CA  USA following the manufacturer\u2019s instruction. M.\u00a0musculus: 2.5\u00a0\u00b5g of total RNA was used with Illumina\u2019s TruSeq RNA sample prep kit.", "GEO Accession:GSM1141110", "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "SINGLE", "ILLUMINA", "Illumina Genome Analyzer IIx", null, "SRP022612", null, null, "Nr72_2y.fastq.gz", "fastq", 1692545916.0, 22270341.0, "GSM1141110 r1", "0:76", "A:447927627;C:393400289;G:405212681;T:445305247;N:700072", 76, null, null, null, 447927627, 393400289, 405212681, 445305247, 700072, "SRX277687", "SRS420787", "SRA076297", "GEO", "Leibniz Institute for Age Research - Fritz Lipmann Institute", 1, 0.88704, null, 0.08604, null, 0.72833, null, 0.47215, null, 76, null, "B", null, "usable mapping rate", "illumina", "early_illumina", "unknown", "cdna_unspecified", "trueseq", "bulk", "bulk", "bulk", null, "Germany", "2013-05-14", "Adult", "Adult", "Skin", "Surface Structure"], [36750, "SRR850599", "SRX277686", "SRS420786", "SRP022612", "PRJNA203029", "RNA seq of Danio rerio and Mus musculus skin for three different age groups", "GSE46916", "Other", "Background: Comparison of temporal gene expression profiles. The RNA seq data comprises 3 age groups: 2  15 month and 30 month for mouse skin; 5  24 month and 42 month for zebrafish skin. Illumina 50bp single stranded single read RNA sequencing Jena Centre for Systems Biology of Ageing   JenAge www.jenage.de Overall design: 15 samples for mouse: 5 biological replicates for 2 month  6 biological replicates for 15 month and 4 biological replicates for 30 month; 20 samples for zebrafish: 9 biological replicates for 5 month  6 biological replicates for 24 month and 5 biological replicates for 42 month", null, "pubmed:26620638", null, "Nr71 danrer skin 24months rep2", "GSM1141109", null, "source name:total RNA extracted from skin|strain:TuAB|age:24 month|tissue:skin", "Nr71 danrer skin 24months rep2", "Illumina Casava software used for extraction of FASTQ files FASTQ files were mapped using Bowtie vs. the respective genomic sequence and a splice site data set created using UCSC's RefSeq annotation for each species Counting of uniquely mapped reads and assignment to RefSeq transcripts/genes using R Statistical Language and Bioconductor Calculation of RPKM values for each transcript and gene from the corresponding RefSeq annotation Genome build: D.\u00a0rerio: danRer7; M. musculus: mm9 Supplementary files format and content: Excel file include raw counts and RPKM values for each sample", "total RNA extracted from skin", null, "D.\u00a0rerio: Skin tissue from male zebrafish was dissected and stored in RNAlater Qiagen  Hilden  Germany at 80 \u00b0C. Total RNA was isolated with TRIzol Life Technologies  Darmstadt  Germany according to the instructions of the manufacturer. M.\u00a0musculus: The isolated skin was homogenized in 500\u00a0\u03bcl QIAzol Qiagen by subsequently adding 100\u00a0\u03bcl chloroform. Following phase separation  the aqueous phase was transferred into a fresh tube  then 0.16 volume NaAc 2\u00a0M  pH\u00a04.0 and 1.1 volume isopropanol were added. The RNA was precipitated by centrifugation and the pellet was washed with 75% ethanol. Total RNA was resuspended in 20\u00a0\u03bcl water GIBCO and stored at \u201380\u00b0C until use. D.\u00a0rerio: 5\u00a0\u00b5g of total RNA was used for preparation of libraries using Illumina\u2019s mRNA Seq sample prep kit Illumina  San Diego  CA  USA following the manufacturer\u2019s instruction. M.\u00a0musculus: 2.5\u00a0\u00b5g of total RNA was used with Illumina\u2019s TruSeq RNA sample prep kit.", null, "strain:TuAB|age:24 month|tissue:skin", "GSM1141109", "GSM1141109: Nr71 danrer skin 24months rep2; Danio rerio; RNA Seq", "GSM1141109 1", null, "1", "D.\u00a0rerio: Skin tissue from male zebrafish was dissected and stored in RNAlater Qiagen  Hilden  Germany at 80 \u00b0C. Total RNA was isolated with TRIzol Life Technologies  Darmstadt  Germany according to the instructions of the manufacturer. M.\u00a0musculus: The isolated skin was homogenized in 500\u00a0\u03bcl QIAzol Qiagen by subsequently adding 100\u00a0\u03bcl chloroform. Following phase separation  the aqueous phase was transferred into a fresh tube  then 0.16 volume NaAc 2\u00a0M  pH\u00a04.0 and 1.1 volume isopropanol were added. The RNA was precipitated by centrifugation and the pellet was washed with 75% ethanol. Total RNA was resuspended in 20\u00a0\u03bcl water GIBCO and stored at \u201380\u00b0C until use. D.\u00a0rerio: 5\u00a0\u00b5g of total RNA was used for preparation of libraries using Illumina\u2019s mRNA Seq sample prep kit Illumina  San Diego  CA  USA following the manufacturer\u2019s instruction. M.\u00a0musculus: 2.5\u00a0\u00b5g of total RNA was used with Illumina\u2019s TruSeq RNA sample prep kit.", "GEO Accession:GSM1141109", "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "SINGLE", "ILLUMINA", "Illumina Genome Analyzer IIx", null, "SRP022612", null, null, "Nr71_2y.fastq.gz", "fastq", 1897129404.0, 24962229.0, "GSM1141109 r1", "0:76", "A:500740954;C:442627409;G:457159802;T:495689680;N:911559", 76, null, null, null, 500740954, 442627409, 457159802, 495689680, 911559, "SRX277686", "SRS420786", "SRA076297", "GEO", "Leibniz Institute for Age Research - Fritz Lipmann Institute", 1, 0.88731, null, 0.07537, null, 0.73628, null, 0.4846, null, 76, null, "B", null, "usable mapping rate", "illumina", "early_illumina", "unknown", "cdna_unspecified", "trueseq", "bulk", "bulk", "bulk", null, "Germany", "2013-05-14", "Adult", "Adult", "Skin", "Surface Structure"], [36751, "SRR850598", "SRX277685", "SRS420785", "SRP022612", "PRJNA203029", "RNA seq of Danio rerio and Mus musculus skin for three different age groups", "GSE46916", "Other", "Background: Comparison of temporal gene expression profiles. The RNA seq data comprises 3 age groups: 2  15 month and 30 month for mouse skin; 5  24 month and 42 month for zebrafish skin. Illumina 50bp single stranded single read RNA sequencing Jena Centre for Systems Biology of Ageing   JenAge www.jenage.de Overall design: 15 samples for mouse: 5 biological replicates for 2 month  6 biological replicates for 15 month and 4 biological replicates for 30 month; 20 samples for zebrafish: 9 biological replicates for 5 month  6 biological replicates for 24 month and 5 biological replicates for 42 month", null, "pubmed:26620638", null, "Nr70 danrer skin 24months rep1", "GSM1141108", null, "source name:total RNA extracted from skin|strain:TuAB|age:24 month|tissue:skin", "Nr70 danrer skin 24months rep1", "Illumina Casava software used for extraction of FASTQ files FASTQ files were mapped using Bowtie vs. the respective genomic sequence and a splice site data set created using UCSC's RefSeq annotation for each species Counting of uniquely mapped reads and assignment to RefSeq transcripts/genes using R Statistical Language and Bioconductor Calculation of RPKM values for each transcript and gene from the corresponding RefSeq annotation Genome build: D.\u00a0rerio: danRer7; M. musculus: mm9 Supplementary files format and content: Excel file include raw counts and RPKM values for each sample", "total RNA extracted from skin", null, "D.\u00a0rerio: Skin tissue from male zebrafish was dissected and stored in RNAlater Qiagen  Hilden  Germany at 80 \u00b0C. Total RNA was isolated with TRIzol Life Technologies  Darmstadt  Germany according to the instructions of the manufacturer. M.\u00a0musculus: The isolated skin was homogenized in 500\u00a0\u03bcl QIAzol Qiagen by subsequently adding 100\u00a0\u03bcl chloroform. Following phase separation  the aqueous phase was transferred into a fresh tube  then 0.16 volume NaAc 2\u00a0M  pH\u00a04.0 and 1.1 volume isopropanol were added. The RNA was precipitated by centrifugation and the pellet was washed with 75% ethanol. Total RNA was resuspended in 20\u00a0\u03bcl water GIBCO and stored at \u201380\u00b0C until use. D.\u00a0rerio: 5\u00a0\u00b5g of total RNA was used for preparation of libraries using Illumina\u2019s mRNA Seq sample prep kit Illumina  San Diego  CA  USA following the manufacturer\u2019s instruction. M.\u00a0musculus: 2.5\u00a0\u00b5g of total RNA was used with Illumina\u2019s TruSeq RNA sample prep kit.", null, "strain:TuAB|age:24 month|tissue:skin", "GSM1141108", "GSM1141108: Nr70 danrer skin 24months rep1; Danio rerio; RNA Seq", "GSM1141108 1", null, "1", "D.\u00a0rerio: Skin tissue from male zebrafish was dissected and stored in RNAlater Qiagen  Hilden  Germany at 80 \u00b0C. Total RNA was isolated with TRIzol Life Technologies  Darmstadt  Germany according to the instructions of the manufacturer. M.\u00a0musculus: The isolated skin was homogenized in 500\u00a0\u03bcl QIAzol Qiagen by subsequently adding 100\u00a0\u03bcl chloroform. Following phase separation  the aqueous phase was transferred into a fresh tube  then 0.16 volume NaAc 2\u00a0M  pH\u00a04.0 and 1.1 volume isopropanol were added. The RNA was precipitated by centrifugation and the pellet was washed with 75% ethanol. Total RNA was resuspended in 20\u00a0\u03bcl water GIBCO and stored at \u201380\u00b0C until use. D.\u00a0rerio: 5\u00a0\u00b5g of total RNA was used for preparation of libraries using Illumina\u2019s mRNA Seq sample prep kit Illumina  San Diego  CA  USA following the manufacturer\u2019s instruction. M.\u00a0musculus: 2.5\u00a0\u00b5g of total RNA was used with Illumina\u2019s TruSeq RNA sample prep kit.", "GEO Accession:GSM1141108", "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "SINGLE", "ILLUMINA", "Illumina Genome Analyzer IIx", null, "SRP022612", null, null, "Nr70_2y.fastq.gz", "fastq", 2239190508.0, 29463033.0, "GSM1141108 r1", "0:76", "A:599665392;C:514113743;G:531871299;T:592730477;N:809597", 76, null, null, null, 599665392, 514113743, 531871299, 592730477, 809597, "SRX277685", "SRS420785", "SRA076297", "GEO", "Leibniz Institute for Age Research - Fritz Lipmann Institute", 1, 0.89167, null, 0.09031, null, 0.73515, null, 0.49129, null, 76, null, "B", null, "usable mapping rate", "illumina", "early_illumina", "unknown", "cdna_unspecified", "trueseq", "bulk", "bulk", "bulk", null, "Germany", "2013-05-14", "Adult", "Adult", "Skin", "Surface Structure"], [36752, "SRR850597", "SRX277684", "SRS420784", "SRP022612", "PRJNA203029", "RNA seq of Danio rerio and Mus musculus skin for three different age groups", "GSE46916", "Other", "Background: Comparison of temporal gene expression profiles. The RNA seq data comprises 3 age groups: 2  15 month and 30 month for mouse skin; 5  24 month and 42 month for zebrafish skin. Illumina 50bp single stranded single read RNA sequencing Jena Centre for Systems Biology of Ageing   JenAge www.jenage.de Overall design: 15 samples for mouse: 5 biological replicates for 2 month  6 biological replicates for 15 month and 4 biological replicates for 30 month; 20 samples for zebrafish: 9 biological replicates for 5 month  6 biological replicates for 24 month and 5 biological replicates for 42 month", null, "pubmed:26620638", null, "Nr30 danrer skin 5months rep9", "GSM1141107", null, "source name:total RNA extracted from skin|strain:TuAB|age:5 month|tissue:skin", "Nr30 danrer skin 5months rep9", "Illumina Casava software used for extraction of FASTQ files FASTQ files were mapped using Bowtie vs. the respective genomic sequence and a splice site data set created using UCSC's RefSeq annotation for each species Counting of uniquely mapped reads and assignment to RefSeq transcripts/genes using R Statistical Language and Bioconductor Calculation of RPKM values for each transcript and gene from the corresponding RefSeq annotation Genome build: D.\u00a0rerio: danRer7; M. musculus: mm9 Supplementary files format and content: Excel file include raw counts and RPKM values for each sample", "total RNA extracted from skin", null, "D.\u00a0rerio: Skin tissue from male zebrafish was dissected and stored in RNAlater Qiagen  Hilden  Germany at 80 \u00b0C. Total RNA was isolated with TRIzol Life Technologies  Darmstadt  Germany according to the instructions of the manufacturer. M.\u00a0musculus: The isolated skin was homogenized in 500\u00a0\u03bcl QIAzol Qiagen by subsequently adding 100\u00a0\u03bcl chloroform. Following phase separation  the aqueous phase was transferred into a fresh tube  then 0.16 volume NaAc 2\u00a0M  pH\u00a04.0 and 1.1 volume isopropanol were added. The RNA was precipitated by centrifugation and the pellet was washed with 75% ethanol. Total RNA was resuspended in 20\u00a0\u03bcl water GIBCO and stored at \u201380\u00b0C until use. D.\u00a0rerio: 5\u00a0\u00b5g of total RNA was used for preparation of libraries using Illumina\u2019s mRNA Seq sample prep kit Illumina  San Diego  CA  USA following the manufacturer\u2019s instruction. M.\u00a0musculus: 2.5\u00a0\u00b5g of total RNA was used with Illumina\u2019s TruSeq RNA sample prep kit.", null, "strain:TuAB|age:5 month|tissue:skin", "GSM1141107", "GSM1141107: Nr30 danrer skin 5months rep9; Danio rerio; RNA Seq", "GSM1141107 1", null, "1", "D.\u00a0rerio: Skin tissue from male zebrafish was dissected and stored in RNAlater Qiagen  Hilden  Germany at 80 \u00b0C. Total RNA was isolated with TRIzol Life Technologies  Darmstadt  Germany according to the instructions of the manufacturer. M.\u00a0musculus: The isolated skin was homogenized in 500\u00a0\u03bcl QIAzol Qiagen by subsequently adding 100\u00a0\u03bcl chloroform. Following phase separation  the aqueous phase was transferred into a fresh tube  then 0.16 volume NaAc 2\u00a0M  pH\u00a04.0 and 1.1 volume isopropanol were added. The RNA was precipitated by centrifugation and the pellet was washed with 75% ethanol. Total RNA was resuspended in 20\u00a0\u03bcl water GIBCO and stored at \u201380\u00b0C until use. D.\u00a0rerio: 5\u00a0\u00b5g of total RNA was used for preparation of libraries using Illumina\u2019s mRNA Seq sample prep kit Illumina  San Diego  CA  USA following the manufacturer\u2019s instruction. M.\u00a0musculus: 2.5\u00a0\u00b5g of total RNA was used with Illumina\u2019s TruSeq RNA sample prep kit.", "GEO Accession:GSM1141107", "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "SINGLE", "ILLUMINA", "Illumina Genome Analyzer IIx", null, "SRP022612", null, null, "Nr30_5m.fastq.gz", "fastq", 2502725752.0, 32930602.0, "GSM1141107 r1", "0:76", "A:684745428;C:570860380;G:565883575;T:679750149;N:1486220", 76, null, null, null, 684745428, 570860380, 565883575, 679750149, 1486220, "SRX277684", "SRS420784", "SRA076297", "GEO", "Leibniz Institute for Age Research - Fritz Lipmann Institute", 1, 0.91789, null, 0.10423, null, 0.7263, null, 0.4909, null, 76, null, "B", null, "usable mapping rate", "illumina", "early_illumina", "unknown", "cdna_unspecified", "trueseq", "bulk", "bulk", "bulk", null, "Germany", "2013-05-14", "Adult", "Adult", "Skin", "Surface Structure"], [36753, "SRR850596", "SRX277683", "SRS420783", "SRP022612", "PRJNA203029", "RNA seq of Danio rerio and Mus musculus skin for three different age groups", "GSE46916", "Other", "Background: Comparison of temporal gene expression profiles. The RNA seq data comprises 3 age groups: 2  15 month and 30 month for mouse skin; 5  24 month and 42 month for zebrafish skin. Illumina 50bp single stranded single read RNA sequencing Jena Centre for Systems Biology of Ageing   JenAge www.jenage.de Overall design: 15 samples for mouse: 5 biological replicates for 2 month  6 biological replicates for 15 month and 4 biological replicates for 30 month; 20 samples for zebrafish: 9 biological replicates for 5 month  6 biological replicates for 24 month and 5 biological replicates for 42 month", null, "pubmed:26620638", null, "Nr29 danrer skin 5months rep8", "GSM1141106", null, "source name:total RNA extracted from skin|strain:TuAB|age:5 month|tissue:skin", "Nr29 danrer skin 5months rep8", "Illumina Casava software used for extraction of FASTQ files FASTQ files were mapped using Bowtie vs. the respective genomic sequence and a splice site data set created using UCSC's RefSeq annotation for each species Counting of uniquely mapped reads and assignment to RefSeq transcripts/genes using R Statistical Language and Bioconductor Calculation of RPKM values for each transcript and gene from the corresponding RefSeq annotation Genome build: D.\u00a0rerio: danRer7; M. musculus: mm9 Supplementary files format and content: Excel file include raw counts and RPKM values for each sample", "total RNA extracted from skin", null, "D.\u00a0rerio: Skin tissue from male zebrafish was dissected and stored in RNAlater Qiagen  Hilden  Germany at 80 \u00b0C. Total RNA was isolated with TRIzol Life Technologies  Darmstadt  Germany according to the instructions of the manufacturer. M.\u00a0musculus: The isolated skin was homogenized in 500\u00a0\u03bcl QIAzol Qiagen by subsequently adding 100\u00a0\u03bcl chloroform. Following phase separation  the aqueous phase was transferred into a fresh tube  then 0.16 volume NaAc 2\u00a0M  pH\u00a04.0 and 1.1 volume isopropanol were added. The RNA was precipitated by centrifugation and the pellet was washed with 75% ethanol. Total RNA was resuspended in 20\u00a0\u03bcl water GIBCO and stored at \u201380\u00b0C until use. D.\u00a0rerio: 5\u00a0\u00b5g of total RNA was used for preparation of libraries using Illumina\u2019s mRNA Seq sample prep kit Illumina  San Diego  CA  USA following the manufacturer\u2019s instruction. M.\u00a0musculus: 2.5\u00a0\u00b5g of total RNA was used with Illumina\u2019s TruSeq RNA sample prep kit.", null, "strain:TuAB|age:5 month|tissue:skin", "GSM1141106", "GSM1141106: Nr29 danrer skin 5months rep8; Danio rerio; RNA Seq", "GSM1141106 1", null, "1", "D.\u00a0rerio: Skin tissue from male zebrafish was dissected and stored in RNAlater Qiagen  Hilden  Germany at 80 \u00b0C. Total RNA was isolated with TRIzol Life Technologies  Darmstadt  Germany according to the instructions of the manufacturer. M.\u00a0musculus: The isolated skin was homogenized in 500\u00a0\u03bcl QIAzol Qiagen by subsequently adding 100\u00a0\u03bcl chloroform. Following phase separation  the aqueous phase was transferred into a fresh tube  then 0.16 volume NaAc 2\u00a0M  pH\u00a04.0 and 1.1 volume isopropanol were added. The RNA was precipitated by centrifugation and the pellet was washed with 75% ethanol. Total RNA was resuspended in 20\u00a0\u03bcl water GIBCO and stored at \u201380\u00b0C until use. D.\u00a0rerio: 5\u00a0\u00b5g of total RNA was used for preparation of libraries using Illumina\u2019s mRNA Seq sample prep kit Illumina  San Diego  CA  USA following the manufacturer\u2019s instruction. M.\u00a0musculus: 2.5\u00a0\u00b5g of total RNA was used with Illumina\u2019s TruSeq RNA sample prep kit.", "GEO Accession:GSM1141106", "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "SINGLE", "ILLUMINA", "Illumina Genome Analyzer IIx", null, "SRP022612", null, null, "Nr29_5m.fastq.gz", "fastq", 2012479468.0, 26479993.0, "GSM1141106 r1", "0:76", "A:558800574;C:450898608;G:447907004;T:553773824;N:1099458", 76, null, null, null, 558800574, 450898608, 447907004, 553773824, 1099458, "SRX277683", "SRS420783", "SRA076297", "GEO", "Leibniz Institute for Age Research - Fritz Lipmann Institute", 1, 0.9055, null, 0.1197, null, 0.7119, null, 0.48553, null, 76, null, "B", null, "usable mapping rate", "illumina", "early_illumina", "unknown", "cdna_unspecified", "trueseq", "bulk", "bulk", "bulk", null, "Germany", "2013-05-14", "Adult", "Adult", "Skin", "Surface Structure"], [36754, "SRR850595", "SRX277682", "SRS420782", "SRP022612", "PRJNA203029", "RNA seq of Danio rerio and Mus musculus skin for three different age groups", "GSE46916", "Other", "Background: Comparison of temporal gene expression profiles. The RNA seq data comprises 3 age groups: 2  15 month and 30 month for mouse skin; 5  24 month and 42 month for zebrafish skin. Illumina 50bp single stranded single read RNA sequencing Jena Centre for Systems Biology of Ageing   JenAge www.jenage.de Overall design: 15 samples for mouse: 5 biological replicates for 2 month  6 biological replicates for 15 month and 4 biological replicates for 30 month; 20 samples for zebrafish: 9 biological replicates for 5 month  6 biological replicates for 24 month and 5 biological replicates for 42 month", null, "pubmed:26620638", null, "Nr28 danrer skin 5months rep7", "GSM1141105", null, "source name:total RNA extracted from skin|strain:TuAB|age:5 month|tissue:skin", "Nr28 danrer skin 5months rep7", "Illumina Casava software used for extraction of FASTQ files FASTQ files were mapped using Bowtie vs. the respective genomic sequence and a splice site data set created using UCSC's RefSeq annotation for each species Counting of uniquely mapped reads and assignment to RefSeq transcripts/genes using R Statistical Language and Bioconductor Calculation of RPKM values for each transcript and gene from the corresponding RefSeq annotation Genome build: D.\u00a0rerio: danRer7; M. musculus: mm9 Supplementary files format and content: Excel file include raw counts and RPKM values for each sample", "total RNA extracted from skin", null, "D.\u00a0rerio: Skin tissue from male zebrafish was dissected and stored in RNAlater Qiagen  Hilden  Germany at 80 \u00b0C. Total RNA was isolated with TRIzol Life Technologies  Darmstadt  Germany according to the instructions of the manufacturer. M.\u00a0musculus: The isolated skin was homogenized in 500\u00a0\u03bcl QIAzol Qiagen by subsequently adding 100\u00a0\u03bcl chloroform. Following phase separation  the aqueous phase was transferred into a fresh tube  then 0.16 volume NaAc 2\u00a0M  pH\u00a04.0 and 1.1 volume isopropanol were added. The RNA was precipitated by centrifugation and the pellet was washed with 75% ethanol. Total RNA was resuspended in 20\u00a0\u03bcl water GIBCO and stored at \u201380\u00b0C until use. D.\u00a0rerio: 5\u00a0\u00b5g of total RNA was used for preparation of libraries using Illumina\u2019s mRNA Seq sample prep kit Illumina  San Diego  CA  USA following the manufacturer\u2019s instruction. M.\u00a0musculus: 2.5\u00a0\u00b5g of total RNA was used with Illumina\u2019s TruSeq RNA sample prep kit.", null, "strain:TuAB|age:5 month|tissue:skin", "GSM1141105", "GSM1141105: Nr28 danrer skin 5months rep7; Danio rerio; RNA Seq", "GSM1141105 1", null, "1", "D.\u00a0rerio: Skin tissue from male zebrafish was dissected and stored in RNAlater Qiagen  Hilden  Germany at 80 \u00b0C. Total RNA was isolated with TRIzol Life Technologies  Darmstadt  Germany according to the instructions of the manufacturer. M.\u00a0musculus: The isolated skin was homogenized in 500\u00a0\u03bcl QIAzol Qiagen by subsequently adding 100\u00a0\u03bcl chloroform. Following phase separation  the aqueous phase was transferred into a fresh tube  then 0.16 volume NaAc 2\u00a0M  pH\u00a04.0 and 1.1 volume isopropanol were added. The RNA was precipitated by centrifugation and the pellet was washed with 75% ethanol. Total RNA was resuspended in 20\u00a0\u03bcl water GIBCO and stored at \u201380\u00b0C until use. D.\u00a0rerio: 5\u00a0\u00b5g of total RNA was used for preparation of libraries using Illumina\u2019s mRNA Seq sample prep kit Illumina  San Diego  CA  USA following the manufacturer\u2019s instruction. M.\u00a0musculus: 2.5\u00a0\u00b5g of total RNA was used with Illumina\u2019s TruSeq RNA sample prep kit.", "GEO Accession:GSM1141105", "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "SINGLE", "ILLUMINA", "Illumina Genome Analyzer IIx", null, "SRP022612", null, null, "Nr28_5m.fastq.gz", "fastq", 2075098224.0, 27303924.0, "GSM1141105 r1", "0:76", "A:555062827;C:487427075;G:487360186;T:544381395;N:866741", 76, null, null, null, 555062827, 487427075, 487360186, 544381395, 866741, "SRX277682", "SRS420782", "SRA076297", "GEO", "Leibniz Institute for Age Research - Fritz Lipmann Institute", 1, 0.91784, null, 0.08866, null, 0.73208, null, 0.47793, null, 76, null, "B", null, "usable mapping rate", "illumina", "early_illumina", "unknown", "cdna_unspecified", "trueseq", "bulk", "bulk", "bulk", null, "Germany", "2013-05-14", "Adult", "Adult", "Skin", "Surface Structure"], [36755, "SRR850594", "SRX277681", "SRS420781", "SRP022612", "PRJNA203029", "RNA seq of Danio rerio and Mus musculus skin for three different age groups", "GSE46916", "Other", "Background: Comparison of temporal gene expression profiles. The RNA seq data comprises 3 age groups: 2  15 month and 30 month for mouse skin; 5  24 month and 42 month for zebrafish skin. Illumina 50bp single stranded single read RNA sequencing Jena Centre for Systems Biology of Ageing   JenAge www.jenage.de Overall design: 15 samples for mouse: 5 biological replicates for 2 month  6 biological replicates for 15 month and 4 biological replicates for 30 month; 20 samples for zebrafish: 9 biological replicates for 5 month  6 biological replicates for 24 month and 5 biological replicates for 42 month", null, "pubmed:26620638", null, "Nr27 danrer skin 5months rep6", "GSM1141104", null, "source name:total RNA extracted from skin|strain:TuAB|age:5 month|tissue:skin", "Nr27 danrer skin 5months rep6", "Illumina Casava software used for extraction of FASTQ files FASTQ files were mapped using Bowtie vs. the respective genomic sequence and a splice site data set created using UCSC's RefSeq annotation for each species Counting of uniquely mapped reads and assignment to RefSeq transcripts/genes using R Statistical Language and Bioconductor Calculation of RPKM values for each transcript and gene from the corresponding RefSeq annotation Genome build: D.\u00a0rerio: danRer7; M. musculus: mm9 Supplementary files format and content: Excel file include raw counts and RPKM values for each sample", "total RNA extracted from skin", null, "D.\u00a0rerio: Skin tissue from male zebrafish was dissected and stored in RNAlater Qiagen  Hilden  Germany at 80 \u00b0C. Total RNA was isolated with TRIzol Life Technologies  Darmstadt  Germany according to the instructions of the manufacturer. M.\u00a0musculus: The isolated skin was homogenized in 500\u00a0\u03bcl QIAzol Qiagen by subsequently adding 100\u00a0\u03bcl chloroform. Following phase separation  the aqueous phase was transferred into a fresh tube  then 0.16 volume NaAc 2\u00a0M  pH\u00a04.0 and 1.1 volume isopropanol were added. The RNA was precipitated by centrifugation and the pellet was washed with 75% ethanol. Total RNA was resuspended in 20\u00a0\u03bcl water GIBCO and stored at \u201380\u00b0C until use. D.\u00a0rerio: 5\u00a0\u00b5g of total RNA was used for preparation of libraries using Illumina\u2019s mRNA Seq sample prep kit Illumina  San Diego  CA  USA following the manufacturer\u2019s instruction. M.\u00a0musculus: 2.5\u00a0\u00b5g of total RNA was used with Illumina\u2019s TruSeq RNA sample prep kit.", null, "strain:TuAB|age:5 month|tissue:skin", "GSM1141104", "GSM1141104: Nr27 danrer skin 5months rep6; Danio rerio; RNA Seq", "GSM1141104 1", null, "1", "D.\u00a0rerio: Skin tissue from male zebrafish was dissected and stored in RNAlater Qiagen  Hilden  Germany at 80 \u00b0C. Total RNA was isolated with TRIzol Life Technologies  Darmstadt  Germany according to the instructions of the manufacturer. M.\u00a0musculus: The isolated skin was homogenized in 500\u00a0\u03bcl QIAzol Qiagen by subsequently adding 100\u00a0\u03bcl chloroform. Following phase separation  the aqueous phase was transferred into a fresh tube  then 0.16 volume NaAc 2\u00a0M  pH\u00a04.0 and 1.1 volume isopropanol were added. The RNA was precipitated by centrifugation and the pellet was washed with 75% ethanol. Total RNA was resuspended in 20\u00a0\u03bcl water GIBCO and stored at \u201380\u00b0C until use. D.\u00a0rerio: 5\u00a0\u00b5g of total RNA was used for preparation of libraries using Illumina\u2019s mRNA Seq sample prep kit Illumina  San Diego  CA  USA following the manufacturer\u2019s instruction. M.\u00a0musculus: 2.5\u00a0\u00b5g of total RNA was used with Illumina\u2019s TruSeq RNA sample prep kit.", "GEO Accession:GSM1141104", "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "SINGLE", "ILLUMINA", "Illumina Genome Analyzer IIx", null, "SRP022612", null, null, "Nr27_5m.fastq.gz", "fastq", 2415354480.0, 31780980.0, "GSM1141104 r1", "0:76", "A:661012615;C:552471296;G:546304105;T:654315033;N:1251431", 76, null, null, null, 661012615, 552471296, 546304105, 654315033, 1251431, "SRX277681", "SRS420781", "SRA076297", "GEO", "Leibniz Institute for Age Research - Fritz Lipmann Institute", 1, 0.91282, null, 0.104, null, 0.7236, null, 0.48186, null, 76, null, "B", null, "usable mapping rate", "illumina", "early_illumina", "unknown", "cdna_unspecified", "trueseq", "bulk", "bulk", "bulk", null, "Germany", "2013-05-14", "Adult", "Adult", "Skin", "Surface Structure"], [36756, "SRR850593", "SRX277680", "SRS420780", "SRP022612", "PRJNA203029", "RNA seq of Danio rerio and Mus musculus skin for three different age groups", "GSE46916", "Other", "Background: Comparison of temporal gene expression profiles. The RNA seq data comprises 3 age groups: 2  15 month and 30 month for mouse skin; 5  24 month and 42 month for zebrafish skin. Illumina 50bp single stranded single read RNA sequencing Jena Centre for Systems Biology of Ageing   JenAge www.jenage.de Overall design: 15 samples for mouse: 5 biological replicates for 2 month  6 biological replicates for 15 month and 4 biological replicates for 30 month; 20 samples for zebrafish: 9 biological replicates for 5 month  6 biological replicates for 24 month and 5 biological replicates for 42 month", null, "pubmed:26620638", null, "Nr26 danrer skin 5months rep5", "GSM1141103", null, "source name:total RNA extracted from skin|strain:TuAB|age:5 month|tissue:skin", "Nr26 danrer skin 5months rep5", "Illumina Casava software used for extraction of FASTQ files FASTQ files were mapped using Bowtie vs. the respective genomic sequence and a splice site data set created using UCSC's RefSeq annotation for each species Counting of uniquely mapped reads and assignment to RefSeq transcripts/genes using R Statistical Language and Bioconductor Calculation of RPKM values for each transcript and gene from the corresponding RefSeq annotation Genome build: D.\u00a0rerio: danRer7; M. musculus: mm9 Supplementary files format and content: Excel file include raw counts and RPKM values for each sample", "total RNA extracted from skin", null, "D.\u00a0rerio: Skin tissue from male zebrafish was dissected and stored in RNAlater Qiagen  Hilden  Germany at 80 \u00b0C. Total RNA was isolated with TRIzol Life Technologies  Darmstadt  Germany according to the instructions of the manufacturer. M.\u00a0musculus: The isolated skin was homogenized in 500\u00a0\u03bcl QIAzol Qiagen by subsequently adding 100\u00a0\u03bcl chloroform. Following phase separation  the aqueous phase was transferred into a fresh tube  then 0.16 volume NaAc 2\u00a0M  pH\u00a04.0 and 1.1 volume isopropanol were added. The RNA was precipitated by centrifugation and the pellet was washed with 75% ethanol. Total RNA was resuspended in 20\u00a0\u03bcl water GIBCO and stored at \u201380\u00b0C until use. D.\u00a0rerio: 5\u00a0\u00b5g of total RNA was used for preparation of libraries using Illumina\u2019s mRNA Seq sample prep kit Illumina  San Diego  CA  USA following the manufacturer\u2019s instruction. M.\u00a0musculus: 2.5\u00a0\u00b5g of total RNA was used with Illumina\u2019s TruSeq RNA sample prep kit.", null, "strain:TuAB|age:5 month|tissue:skin", "GSM1141103", "GSM1141103: Nr26 danrer skin 5months rep5; Danio rerio; RNA Seq", "GSM1141103 1", null, "1", "D.\u00a0rerio: Skin tissue from male zebrafish was dissected and stored in RNAlater Qiagen  Hilden  Germany at 80 \u00b0C. Total RNA was isolated with TRIzol Life Technologies  Darmstadt  Germany according to the instructions of the manufacturer. M.\u00a0musculus: The isolated skin was homogenized in 500\u00a0\u03bcl QIAzol Qiagen by subsequently adding 100\u00a0\u03bcl chloroform. Following phase separation  the aqueous phase was transferred into a fresh tube  then 0.16 volume NaAc 2\u00a0M  pH\u00a04.0 and 1.1 volume isopropanol were added. The RNA was precipitated by centrifugation and the pellet was washed with 75% ethanol. Total RNA was resuspended in 20\u00a0\u03bcl water GIBCO and stored at \u201380\u00b0C until use. D.\u00a0rerio: 5\u00a0\u00b5g of total RNA was used for preparation of libraries using Illumina\u2019s mRNA Seq sample prep kit Illumina  San Diego  CA  USA following the manufacturer\u2019s instruction. M.\u00a0musculus: 2.5\u00a0\u00b5g of total RNA was used with Illumina\u2019s TruSeq RNA sample prep kit.", "GEO Accession:GSM1141103", "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "SINGLE", "ILLUMINA", "Illumina Genome Analyzer IIx", null, "SRP022612", null, null, "Nr26_5m_l8_export.fq.gz", "fastq", 1866135464.0, 24554414.0, "GSM1141103 r1", "0:76", "A:495417130;C:442746432;G:436942898;T:490685510;N:343494", 76, null, null, null, 495417130, 442746432, 436942898, 490685510, 343494, "SRX277680", "SRS420780", "SRA076297", "GEO", "Leibniz Institute for Age Research - Fritz Lipmann Institute", 1, 0.92443, null, 0.08023, null, 0.7288, null, 0.48902, null, 76, null, "B", null, "usable mapping rate", "illumina", "early_illumina", "unknown", "cdna_unspecified", "trueseq", "bulk", "bulk", "bulk", null, "Germany", "2013-05-14", "Adult", "Adult", "Skin", "Surface Structure"], [36757, "SRR850592", "SRX277679", "SRS420778", "SRP022612", "PRJNA203029", "RNA seq of Danio rerio and Mus musculus skin for three different age groups", "GSE46916", "Other", "Background: Comparison of temporal gene expression profiles. The RNA seq data comprises 3 age groups: 2  15 month and 30 month for mouse skin; 5  24 month and 42 month for zebrafish skin. Illumina 50bp single stranded single read RNA sequencing Jena Centre for Systems Biology of Ageing   JenAge www.jenage.de Overall design: 15 samples for mouse: 5 biological replicates for 2 month  6 biological replicates for 15 month and 4 biological replicates for 30 month; 20 samples for zebrafish: 9 biological replicates for 5 month  6 biological replicates for 24 month and 5 biological replicates for 42 month", null, "pubmed:26620638", null, "Nr25 danrer skin 5months rep4", "GSM1141102", null, "source name:total RNA extracted from skin|strain:TuAB|age:5 month|tissue:skin", "Nr25 danrer skin 5months rep4", "Illumina Casava software used for extraction of FASTQ files FASTQ files were mapped using Bowtie vs. the respective genomic sequence and a splice site data set created using UCSC's RefSeq annotation for each species Counting of uniquely mapped reads and assignment to RefSeq transcripts/genes using R Statistical Language and Bioconductor Calculation of RPKM values for each transcript and gene from the corresponding RefSeq annotation Genome build: D.\u00a0rerio: danRer7; M. musculus: mm9 Supplementary files format and content: Excel file include raw counts and RPKM values for each sample", "total RNA extracted from skin", null, "D.\u00a0rerio: Skin tissue from male zebrafish was dissected and stored in RNAlater Qiagen  Hilden  Germany at 80 \u00b0C. Total RNA was isolated with TRIzol Life Technologies  Darmstadt  Germany according to the instructions of the manufacturer. M.\u00a0musculus: The isolated skin was homogenized in 500\u00a0\u03bcl QIAzol Qiagen by subsequently adding 100\u00a0\u03bcl chloroform. Following phase separation  the aqueous phase was transferred into a fresh tube  then 0.16 volume NaAc 2\u00a0M  pH\u00a04.0 and 1.1 volume isopropanol were added. The RNA was precipitated by centrifugation and the pellet was washed with 75% ethanol. Total RNA was resuspended in 20\u00a0\u03bcl water GIBCO and stored at \u201380\u00b0C until use. D.\u00a0rerio: 5\u00a0\u00b5g of total RNA was used for preparation of libraries using Illumina\u2019s mRNA Seq sample prep kit Illumina  San Diego  CA  USA following the manufacturer\u2019s instruction. M.\u00a0musculus: 2.5\u00a0\u00b5g of total RNA was used with Illumina\u2019s TruSeq RNA sample prep kit.", null, "strain:TuAB|age:5 month|tissue:skin", "GSM1141102", "GSM1141102: Nr25 danrer skin 5months rep4; Danio rerio; RNA Seq", "GSM1141102 1", null, "1", "D.\u00a0rerio: Skin tissue from male zebrafish was dissected and stored in RNAlater Qiagen  Hilden  Germany at 80 \u00b0C. Total RNA was isolated with TRIzol Life Technologies  Darmstadt  Germany according to the instructions of the manufacturer. M.\u00a0musculus: The isolated skin was homogenized in 500\u00a0\u03bcl QIAzol Qiagen by subsequently adding 100\u00a0\u03bcl chloroform. Following phase separation  the aqueous phase was transferred into a fresh tube  then 0.16 volume NaAc 2\u00a0M  pH\u00a04.0 and 1.1 volume isopropanol were added. The RNA was precipitated by centrifugation and the pellet was washed with 75% ethanol. Total RNA was resuspended in 20\u00a0\u03bcl water GIBCO and stored at \u201380\u00b0C until use. D.\u00a0rerio: 5\u00a0\u00b5g of total RNA was used for preparation of libraries using Illumina\u2019s mRNA Seq sample prep kit Illumina  San Diego  CA  USA following the manufacturer\u2019s instruction. M.\u00a0musculus: 2.5\u00a0\u00b5g of total RNA was used with Illumina\u2019s TruSeq RNA sample prep kit.", "GEO Accession:GSM1141102", "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "SINGLE", "ILLUMINA", "Illumina Genome Analyzer IIx", null, "SRP022612", null, null, "Nr25_5m_l7_export.fq.gz", "fastq", 2146470508.0, 28243033.0, "GSM1141102 r1", "0:76", "A:583770580;C:491677255;G:491536604;T:579365742;N:120327", 76, null, null, null, 583770580, 491677255, 491536604, 579365742, 120327, "SRX277679", "SRS420778", "SRA076297", "GEO", "Leibniz Institute for Age Research - Fritz Lipmann Institute", 1, 0.89568, null, 0.11303, null, 0.71778, null, 0.47882, null, 76, null, "B", null, "usable mapping rate", "illumina", "early_illumina", "unknown", "cdna_unspecified", "trueseq", "bulk", "bulk", "bulk", null, "Germany", "2013-05-14", "Adult", "Adult", "Skin", "Surface Structure"], [36758, "SRR850591", "SRX277678", "SRS420779", "SRP022612", "PRJNA203029", "RNA seq of Danio rerio and Mus musculus skin for three different age groups", "GSE46916", "Other", "Background: Comparison of temporal gene expression profiles. The RNA seq data comprises 3 age groups: 2  15 month and 30 month for mouse skin; 5  24 month and 42 month for zebrafish skin. Illumina 50bp single stranded single read RNA sequencing Jena Centre for Systems Biology of Ageing   JenAge www.jenage.de Overall design: 15 samples for mouse: 5 biological replicates for 2 month  6 biological replicates for 15 month and 4 biological replicates for 30 month; 20 samples for zebrafish: 9 biological replicates for 5 month  6 biological replicates for 24 month and 5 biological replicates for 42 month", null, "pubmed:26620638", null, "Nr23 danrer skin 5months rep3", "GSM1141101", null, "source name:total RNA extracted from skin|strain:TuAB|age:5 month|tissue:skin", "Nr23 danrer skin 5months rep3", "Illumina Casava software used for extraction of FASTQ files FASTQ files were mapped using Bowtie vs. the respective genomic sequence and a splice site data set created using UCSC's RefSeq annotation for each species Counting of uniquely mapped reads and assignment to RefSeq transcripts/genes using R Statistical Language and Bioconductor Calculation of RPKM values for each transcript and gene from the corresponding RefSeq annotation Genome build: D.\u00a0rerio: danRer7; M. musculus: mm9 Supplementary files format and content: Excel file include raw counts and RPKM values for each sample", "total RNA extracted from skin", null, "D.\u00a0rerio: Skin tissue from male zebrafish was dissected and stored in RNAlater Qiagen  Hilden  Germany at 80 \u00b0C. Total RNA was isolated with TRIzol Life Technologies  Darmstadt  Germany according to the instructions of the manufacturer. M.\u00a0musculus: The isolated skin was homogenized in 500\u00a0\u03bcl QIAzol Qiagen by subsequently adding 100\u00a0\u03bcl chloroform. Following phase separation  the aqueous phase was transferred into a fresh tube  then 0.16 volume NaAc 2\u00a0M  pH\u00a04.0 and 1.1 volume isopropanol were added. The RNA was precipitated by centrifugation and the pellet was washed with 75% ethanol. Total RNA was resuspended in 20\u00a0\u03bcl water GIBCO and stored at \u201380\u00b0C until use. D.\u00a0rerio: 5\u00a0\u00b5g of total RNA was used for preparation of libraries using Illumina\u2019s mRNA Seq sample prep kit Illumina  San Diego  CA  USA following the manufacturer\u2019s instruction. M.\u00a0musculus: 2.5\u00a0\u00b5g of total RNA was used with Illumina\u2019s TruSeq RNA sample prep kit.", null, "strain:TuAB|age:5 month|tissue:skin", "GSM1141101", "GSM1141101: Nr23 danrer skin 5months rep3; Danio rerio; RNA Seq", "GSM1141101 1", null, "1", "D.\u00a0rerio: Skin tissue from male zebrafish was dissected and stored in RNAlater Qiagen  Hilden  Germany at 80 \u00b0C. Total RNA was isolated with TRIzol Life Technologies  Darmstadt  Germany according to the instructions of the manufacturer. M.\u00a0musculus: The isolated skin was homogenized in 500\u00a0\u03bcl QIAzol Qiagen by subsequently adding 100\u00a0\u03bcl chloroform. Following phase separation  the aqueous phase was transferred into a fresh tube  then 0.16 volume NaAc 2\u00a0M  pH\u00a04.0 and 1.1 volume isopropanol were added. The RNA was precipitated by centrifugation and the pellet was washed with 75% ethanol. Total RNA was resuspended in 20\u00a0\u03bcl water GIBCO and stored at \u201380\u00b0C until use. D.\u00a0rerio: 5\u00a0\u00b5g of total RNA was used for preparation of libraries using Illumina\u2019s mRNA Seq sample prep kit Illumina  San Diego  CA  USA following the manufacturer\u2019s instruction. M.\u00a0musculus: 2.5\u00a0\u00b5g of total RNA was used with Illumina\u2019s TruSeq RNA sample prep kit.", "GEO Accession:GSM1141101", "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "SINGLE", "ILLUMINA", "Illumina Genome Analyzer IIx", null, "SRP022612", null, null, "Nr23_5m_l6_export.fq.gz", "fastq", 2628691268.0, 34588043.0, "GSM1141101 r1", "0:76", "A:693944705;C:617538873;G:611502862;T:705582669;N:122159", 76, null, null, null, 693944705, 617538873, 611502862, 705582669, 122159, "SRX277678", "SRS420779", "SRA076297", "GEO", "Leibniz Institute for Age Research - Fritz Lipmann Institute", 1, 0.92203, null, 0.09747, null, 0.71378, null, 0.47796, null, 76, null, "B", null, "usable mapping rate", "illumina", "early_illumina", "unknown", "cdna_unspecified", "trueseq", "bulk", "bulk", "bulk", null, "Germany", "2013-05-14", "Adult", "Adult", "Skin", "Surface Structure"], [36759, "SRR850590", "SRX277677", "SRS420777", "SRP022612", "PRJNA203029", "RNA seq of Danio rerio and Mus musculus skin for three different age groups", "GSE46916", "Other", "Background: Comparison of temporal gene expression profiles. The RNA seq data comprises 3 age groups: 2  15 month and 30 month for mouse skin; 5  24 month and 42 month for zebrafish skin. Illumina 50bp single stranded single read RNA sequencing Jena Centre for Systems Biology of Ageing   JenAge www.jenage.de Overall design: 15 samples for mouse: 5 biological replicates for 2 month  6 biological replicates for 15 month and 4 biological replicates for 30 month; 20 samples for zebrafish: 9 biological replicates for 5 month  6 biological replicates for 24 month and 5 biological replicates for 42 month", null, "pubmed:26620638", null, "Nr22 danrer skin 5months rep2", "GSM1141100", null, "source name:total RNA extracted from skin|strain:TuAB|age:5 month|tissue:skin", "Nr22 danrer skin 5months rep2", "Illumina Casava software used for extraction of FASTQ files FASTQ files were mapped using Bowtie vs. the respective genomic sequence and a splice site data set created using UCSC's RefSeq annotation for each species Counting of uniquely mapped reads and assignment to RefSeq transcripts/genes using R Statistical Language and Bioconductor Calculation of RPKM values for each transcript and gene from the corresponding RefSeq annotation Genome build: D.\u00a0rerio: danRer7; M. musculus: mm9 Supplementary files format and content: Excel file include raw counts and RPKM values for each sample", "total RNA extracted from skin", null, "D.\u00a0rerio: Skin tissue from male zebrafish was dissected and stored in RNAlater Qiagen  Hilden  Germany at 80 \u00b0C. Total RNA was isolated with TRIzol Life Technologies  Darmstadt  Germany according to the instructions of the manufacturer. M.\u00a0musculus: The isolated skin was homogenized in 500\u00a0\u03bcl QIAzol Qiagen by subsequently adding 100\u00a0\u03bcl chloroform. Following phase separation  the aqueous phase was transferred into a fresh tube  then 0.16 volume NaAc 2\u00a0M  pH\u00a04.0 and 1.1 volume isopropanol were added. The RNA was precipitated by centrifugation and the pellet was washed with 75% ethanol. Total RNA was resuspended in 20\u00a0\u03bcl water GIBCO and stored at \u201380\u00b0C until use. D.\u00a0rerio: 5\u00a0\u00b5g of total RNA was used for preparation of libraries using Illumina\u2019s mRNA Seq sample prep kit Illumina  San Diego  CA  USA following the manufacturer\u2019s instruction. M.\u00a0musculus: 2.5\u00a0\u00b5g of total RNA was used with Illumina\u2019s TruSeq RNA sample prep kit.", null, "strain:TuAB|age:5 month|tissue:skin", "GSM1141100", "GSM1141100: Nr22 danrer skin 5months rep2; Danio rerio; RNA Seq", "GSM1141100 1", null, "1", "D.\u00a0rerio: Skin tissue from male zebrafish was dissected and stored in RNAlater Qiagen  Hilden  Germany at 80 \u00b0C. Total RNA was isolated with TRIzol Life Technologies  Darmstadt  Germany according to the instructions of the manufacturer. M.\u00a0musculus: The isolated skin was homogenized in 500\u00a0\u03bcl QIAzol Qiagen by subsequently adding 100\u00a0\u03bcl chloroform. Following phase separation  the aqueous phase was transferred into a fresh tube  then 0.16 volume NaAc 2\u00a0M  pH\u00a04.0 and 1.1 volume isopropanol were added. The RNA was precipitated by centrifugation and the pellet was washed with 75% ethanol. Total RNA was resuspended in 20\u00a0\u03bcl water GIBCO and stored at \u201380\u00b0C until use. D.\u00a0rerio: 5\u00a0\u00b5g of total RNA was used for preparation of libraries using Illumina\u2019s mRNA Seq sample prep kit Illumina  San Diego  CA  USA following the manufacturer\u2019s instruction. M.\u00a0musculus: 2.5\u00a0\u00b5g of total RNA was used with Illumina\u2019s TruSeq RNA sample prep kit.", "GEO Accession:GSM1141100", "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "SINGLE", "ILLUMINA", "Illumina Genome Analyzer IIx", null, "SRP022612", null, null, "Nr22_5m.fastq.gz", "fastq", 3561382724.0, 46860299.0, "GSM1141100 r1", "0:76", "A:956105654;C:826498432;G:826128735;T:952426883;N:223020", 76, null, null, null, 956105654, 826498432, 826128735, 952426883, 223020, "SRX277677", "SRS420777", "SRA076297", "GEO", "Leibniz Institute for Age Research - Fritz Lipmann Institute", 1, 0.93192, null, 0.09055, null, 0.72876, null, 0.52416, null, 76, null, "B", null, "usable mapping rate", "illumina", "early_illumina", "unknown", "cdna_unspecified", "trueseq", "bulk", "bulk", "bulk", null, "Germany", "2013-05-14", "Adult", "Adult", "Skin", "Surface Structure"], [36760, "SRR850589", "SRX277676", "SRS420776", "SRP022612", "PRJNA203029", "RNA seq of Danio rerio and Mus musculus skin for three different age groups", "GSE46916", "Other", "Background: Comparison of temporal gene expression profiles. The RNA seq data comprises 3 age groups: 2  15 month and 30 month for mouse skin; 5  24 month and 42 month for zebrafish skin. Illumina 50bp single stranded single read RNA sequencing Jena Centre for Systems Biology of Ageing   JenAge www.jenage.de Overall design: 15 samples for mouse: 5 biological replicates for 2 month  6 biological replicates for 15 month and 4 biological replicates for 30 month; 20 samples for zebrafish: 9 biological replicates for 5 month  6 biological replicates for 24 month and 5 biological replicates for 42 month", null, "pubmed:26620638", null, "Nr21 danrer skin 5months rep1", "GSM1141099", null, "source name:total RNA extracted from skin|strain:TuAB|age:5 month|tissue:skin", "Nr21 danrer skin 5months rep1", "Illumina Casava software used for extraction of FASTQ files FASTQ files were mapped using Bowtie vs. the respective genomic sequence and a splice site data set created using UCSC's RefSeq annotation for each species Counting of uniquely mapped reads and assignment to RefSeq transcripts/genes using R Statistical Language and Bioconductor Calculation of RPKM values for each transcript and gene from the corresponding RefSeq annotation Genome build: D.\u00a0rerio: danRer7; M. musculus: mm9 Supplementary files format and content: Excel file include raw counts and RPKM values for each sample", "total RNA extracted from skin", null, "D.\u00a0rerio: Skin tissue from male zebrafish was dissected and stored in RNAlater Qiagen  Hilden  Germany at 80 \u00b0C. Total RNA was isolated with TRIzol Life Technologies  Darmstadt  Germany according to the instructions of the manufacturer. M.\u00a0musculus: The isolated skin was homogenized in 500\u00a0\u03bcl QIAzol Qiagen by subsequently adding 100\u00a0\u03bcl chloroform. Following phase separation  the aqueous phase was transferred into a fresh tube  then 0.16 volume NaAc 2\u00a0M  pH\u00a04.0 and 1.1 volume isopropanol were added. The RNA was precipitated by centrifugation and the pellet was washed with 75% ethanol. Total RNA was resuspended in 20\u00a0\u03bcl water GIBCO and stored at \u201380\u00b0C until use. D.\u00a0rerio: 5\u00a0\u00b5g of total RNA was used for preparation of libraries using Illumina\u2019s mRNA Seq sample prep kit Illumina  San Diego  CA  USA following the manufacturer\u2019s instruction. M.\u00a0musculus: 2.5\u00a0\u00b5g of total RNA was used with Illumina\u2019s TruSeq RNA sample prep kit.", null, "strain:TuAB|age:5 month|tissue:skin", "GSM1141099", "GSM1141099: Nr21 danrer skin 5months rep1; Danio rerio; RNA Seq", "GSM1141099 1", null, "1", "D.\u00a0rerio: Skin tissue from male zebrafish was dissected and stored in RNAlater Qiagen  Hilden  Germany at 80 \u00b0C. Total RNA was isolated with TRIzol Life Technologies  Darmstadt  Germany according to the instructions of the manufacturer. M.\u00a0musculus: The isolated skin was homogenized in 500\u00a0\u03bcl QIAzol Qiagen by subsequently adding 100\u00a0\u03bcl chloroform. Following phase separation  the aqueous phase was transferred into a fresh tube  then 0.16 volume NaAc 2\u00a0M  pH\u00a04.0 and 1.1 volume isopropanol were added. The RNA was precipitated by centrifugation and the pellet was washed with 75% ethanol. Total RNA was resuspended in 20\u00a0\u03bcl water GIBCO and stored at \u201380\u00b0C until use. D.\u00a0rerio: 5\u00a0\u00b5g of total RNA was used for preparation of libraries using Illumina\u2019s mRNA Seq sample prep kit Illumina  San Diego  CA  USA following the manufacturer\u2019s instruction. M.\u00a0musculus: 2.5\u00a0\u00b5g of total RNA was used with Illumina\u2019s TruSeq RNA sample prep kit.", "GEO Accession:GSM1141099", "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "SINGLE", "ILLUMINA", "Illumina Genome Analyzer IIx", null, "SRP022612", null, null, "Nr21_5m_l4_export.fq.gz", "fastq", 2709932304.0, 35657004.0, "GSM1141099 r1", "0:76", "A:730073083;C:631072920;G:619401848;T:729203191;N:181262", 76, null, null, null, 730073083, 631072920, 619401848, 729203191, 181262, "SRX277676", "SRS420776", "SRA076297", "GEO", "Leibniz Institute for Age Research - Fritz Lipmann Institute", 1, 0.92839, null, 0.10379, null, 0.71392, null, 0.4865, null, 76, null, "B", null, "usable mapping rate", "illumina", "early_illumina", "unknown", "cdna_unspecified", "trueseq", "bulk", "bulk", "bulk", null, "Germany", "2013-05-14", "Adult", "Adult", "Skin", "Surface Structure"]], "truncated": false, "filtered_table_rows_count": 20, "expanded_columns": [], "expandable_columns": [], "columns": ["rowid", "run.accession", "experiment.accession", "sample.accession", "study.accession", "bioproject", "study.title", "study.alias", "study.type", "study.abstract", "study.attributes", "study.PMIDs", "sample.description", "sample.title", "sample.alias", "sample.centername", "sample.attributes", "GEOsample.title", "GEOsample.dataprocessing", "GEOsample.source", "GEOsample.treatmentprotocol", "GEOsample.extractprotocol", "GEOsample.growthprotocol", "GEOsample.characteristics", "GEOsample.accession", "experiment.title", "experiment.alias", "experiment.library_name", "experiment.design_description", "experiment.library_construction_protocol", "experiment.attributes", "experiment.library_strategy", "experiment.library_source", "experiment.library_selection", "experiment.library_layout", "experiment.platform", "experiment.instrument_model", "experiment.spot_descriptor", "experiment.study_ref", "run.title", "run.attributes", "run.filename", "run.semantic_name", "run.total_bases", "run.total_spots", "run.alias", "run.read_lengths", "run.base_counts", "run.r1_length", "run.r2_length", "run.r3_length", "run.r4_length", "run.Acount", "run.Ccount", "run.Gcount", "run.Tcount", "run.Ncount", "run.experiment", "run.pool_member", "submission.accession", "submission.srasource", "submission.bioprojectsource", "seqdetective.n_mates", "seqdetective.mapping_rate.mate1", "seqdetective.mapping_rate.mate2", "seqdetective.nofeature_rate.mate1", "seqdetective.nofeature_rate.mate2", "seqdetective.sparsity.mate1", "seqdetective.sparsity.mate2", "seqdetective.pos_strand_rate.mate1", "seqdetective.pos_strand_rate.mate2", "seqdetective.readlen.mate1", "seqdetective.readlen.mate2", "seqdetective.judgement.mate1", "seqdetective.judgement.mate2", "seqdetective.judgement.reason", "platform_family", "instrument_generation", "read_bias", "selection_class", "prep_kit", "sc_or_bulk", "tech_class", "technology", "tech_variant", "submission.bioprojectsource.country", "earliest_date", "devstage_curation", "devstage_curation_coarse", "tissue_curation", "tissue_curation_coarse"], "primary_keys": [], "units": {}, "query": {"sql": "select rowid, [run.accession], [experiment.accession], [sample.accession], [study.accession], bioproject, [study.title], [study.alias], [study.type], [study.abstract], [study.attributes], [study.PMIDs], [sample.description], [sample.title], [sample.alias], [sample.centername], [sample.attributes], [GEOsample.title], [GEOsample.dataprocessing], [GEOsample.source], [GEOsample.treatmentprotocol], [GEOsample.extractprotocol], [GEOsample.growthprotocol], [GEOsample.characteristics], [GEOsample.accession], [experiment.title], [experiment.alias], [experiment.library_name], [experiment.design_description], [experiment.library_construction_protocol], [experiment.attributes], [experiment.library_strategy], [experiment.library_source], [experiment.library_selection], [experiment.library_layout], [experiment.platform], [experiment.instrument_model], [experiment.spot_descriptor], [experiment.study_ref], [run.title], [run.attributes], [run.filename], [run.semantic_name], [run.total_bases], [run.total_spots], [run.alias], [run.read_lengths], [run.base_counts], [run.r1_length], [run.r2_length], [run.r3_length], [run.r4_length], [run.Acount], [run.Ccount], [run.Gcount], [run.Tcount], [run.Ncount], [run.experiment], [run.pool_member], [submission.accession], [submission.srasource], [submission.bioprojectsource], [seqdetective.n_mates], [seqdetective.mapping_rate.mate1], [seqdetective.mapping_rate.mate2], [seqdetective.nofeature_rate.mate1], [seqdetective.nofeature_rate.mate2], [seqdetective.sparsity.mate1], [seqdetective.sparsity.mate2], [seqdetective.pos_strand_rate.mate1], [seqdetective.pos_strand_rate.mate2], [seqdetective.readlen.mate1], [seqdetective.readlen.mate2], [seqdetective.judgement.mate1], [seqdetective.judgement.mate2], [seqdetective.judgement.reason], platform_family, instrument_generation, read_bias, selection_class, prep_kit, sc_or_bulk, tech_class, technology, tech_variant, [submission.bioprojectsource.country], earliest_date, devstage_curation, devstage_curation_coarse, tissue_curation, tissue_curation_coarse from run_metadata where \"experiment.library_layout\" = :p0 and \"technology\" = :p1 and \"tissue_curation\" = :p2 order by rowid limit 101", "params": {"p0": "SINGLE", "p1": "bulk", "p2": "Skin"}}, "facet_results": {"experiment.library_strategy": {"name": "experiment.library_strategy", "type": "column", "hideable": false, "toggle_url": "/metadata/run_metadata.json?experiment.library_layout=SINGLE&technology=bulk&tissue_curation=Skin", "results": [{"value": "RNA-Seq", "label": "RNA-Seq", "count": 20, "toggle_url": "http://metadata.rnaquarium.org/metadata/run_metadata.json?experiment.library_layout=SINGLE&technology=bulk&tissue_curation=Skin&experiment.library_strategy=RNA-Seq", "selected": false}], "truncated": false}, "experiment.library_source": {"name": "experiment.library_source", 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