{"database": "metadata", "table": "run_metadata", "is_view": false, "human_description_en": "where experiment.library_layout = \"SINGLE\", experiment.library_selection = \"Oligo-dT\" and experiment.platform = \"BGISEQ\"", "rows": [[57271, "SRR12577970", "SRX9064853", "SRS7314026", "SRP279881", "PRJNA612371", "Danio rerio strain:TU Raw sequence reads", "PRJNA612371", "Whole Genome Sequencing", "Hemorrhage stroke is a severe vascular disease of the brain with a high mortality rate in humans. Sodium tanshinone IIA sulfonate STS is a water soluble derivative of tanshinone IIA  which is the main active ingredient of Salvia miltiorrhiza Bge known as Danshen in Chinese and has been approved as a commercial drug for treating cardiovascular disease by the China Food and Drug Administration. In our previous study  we established a HMG COA inhibitor atorvastatin Ator induced zebrafish model of cerebral hemorrhage and found that STS dramatically decreased both the hemorrhage rate and hemorrhage area  although the underlying mechanism was not fully elucidated. Therefore  in the present study  we conducted transcriptome analysis of the protective effect of STS against Ator induced cerebral hemorrhage in zebrafish using RNA Seq technology  and further clarify its underlying molecular mechanisms on HIF 1 and its regulators  i.e.  the PI3K/Akt and MAPK signaling pathways were verified by real time PCR analysis and specific pharmacological inhibitors. We are also able to show that hemoglobin  carbonic anhydrase  Na+/H+ exchanger and HIF 1 genes might be potential biomarkers of Ator induced cerebral hemorrhage in zebrafish  as well as pharmacological targets of STS. 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We are also able to show that hemoglobin  carbonic anhydrase  Na+/H+ exchanger and HIF 1 genes might be potential biomarkers of Ator induced cerebral hemorrhage in zebrafish  as well as pharmacological targets of STS. 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We are also able to show that hemoglobin  carbonic anhydrase  Na+/H+ exchanger and HIF 1 genes might be potential biomarkers of Ator induced cerebral hemorrhage in zebrafish  as well as pharmacological targets of STS. 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We are also able to show that hemoglobin  carbonic anhydrase  Na+/H+ exchanger and HIF 1 genes might be potential biomarkers of Ator induced cerebral hemorrhage in zebrafish  as well as pharmacological targets of STS. 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We are also able to show that hemoglobin  carbonic anhydrase  Na+/H+ exchanger and HIF 1 genes might be potential biomarkers of Ator induced cerebral hemorrhage in zebrafish  as well as pharmacological targets of STS. 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We are also able to show that hemoglobin  carbonic anhydrase  Na+/H+ exchanger and HIF 1 genes might be potential biomarkers of Ator induced cerebral hemorrhage in zebrafish  as well as pharmacological targets of STS. 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We are also able to show that hemoglobin  carbonic anhydrase  Na+/H+ exchanger and HIF 1 genes might be potential biomarkers of Ator induced cerebral hemorrhage in zebrafish  as well as pharmacological targets of STS. 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ENF and CAR on metabolic regulation during development", null, null, null, null, "3enf", null, "strain:Tu|isolate:N/A|breed:N/A|cultivar:N/A|ecotype:N/A|age:9 96hpf|dev stage:larvae|sex:male and female|tissue:larvae|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio :enrofloxacin group", "enf 3", "enf 3", "96hpf zebrafish larvae enrofloxacin group", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "SINGLE", "BGISEQ", "BGISEQ-500", null, "SRP346547", null, null, "enf_3.fq.gz", "fastq", 3304972350.0, 22033149.0, "enf 3.fq.gz", "0:150 1:0", "A:899427202;C:745999066;G:759917309;T:899625137;N:3636", 150, 0, null, null, 899427202, 745999066, 759917309, 899625137, 3636, "SRX13156591", "SRS11088495", "SRA1329868", "China Agricultural University|College of animal science and technology", "China Agricultural University", 1, 0.94353, null, 0.10795, null, 0.66212, null, 0.47773, null, 150, null, "B", null, "usable mapping rate", "bgi", "bgi", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2021-11-17", "Larval", "Larval", "Undetermined", "Undetermined"], [66967, "SRR16965146", "SRX13156589", "SRS11088492", "SRP346547", "PRJNA780808", "transcriptomics of carbendazim and enrofloxacin on zebrafish embryos", "PRJNA780808", "Other", "reveal a interaction between ENF and CAR on metabolic regulation during development", null, null, null, null, "3car", null, "strain:Tu|isolate:N/A|breed:N/A|cultivar:N/A|ecotype:N/A|age:6 96hpf|dev stage:larvae|sex:male and female|tissue:larvae|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio :carbendazim group", "car 3", "car 3", "96hpf zebrafish larvae carbendazim group", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "SINGLE", "BGISEQ", "BGISEQ-500", null, "SRP346547", null, null, "car_3_1.fq.gz", "fastq", 3335622150.0, 22237481.0, "car 3 1.fq.gz", "0:150 1:0", "A:853229671;C:807433601;G:820478265;T:854475092;N:5521", 150, 0, null, null, 853229671, 807433601, 820478265, 854475092, 5521, "SRX13156589", "SRS11088492", "SRA1329868", "China Agricultural University|College of animal science and technology", "China Agricultural University", 1, 0.96567, null, 0.03093, null, 0.70997, null, 0.47751, null, 150, null, "B", null, "usable mapping rate", "bgi", "bgi", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2021-11-17", "Larval", "Larval", "Undetermined", "Undetermined"], [66968, "SRR16965147", "SRX13156588", "SRS11088494", "SRP346547", "PRJNA780808", "transcriptomics of carbendazim and enrofloxacin on zebrafish embryos", "PRJNA780808", "Other", "reveal a interaction between ENF and CAR on metabolic regulation during development", null, null, null, null, "1car", null, "strain:Tu|isolate:N/A|breed:N/A|cultivar:N/A|ecotype:N/A|age:4 96hpf|dev stage:larvae|sex:male and female|tissue:larvae|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio :carbendazim group", "car 1", "car 1", "96hpf zebrafish larvae carbendazim group", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "SINGLE", "BGISEQ", "BGISEQ-500", null, "SRP346547", null, null, "car_1_1.fq.gz", "fastq", 3378056400.0, 22520376.0, "car 1 1.fq.gz", "0:150 1:0", "A:886009175;C:795203398;G:809447342;T:887390300;N:6185", 150, 0, null, null, 886009175, 795203398, 809447342, 887390300, 6185, "SRX13156588", "SRS11088494", "SRA1329868", "China Agricultural University|College of animal science and technology", "China Agricultural University", 1, 0.95701, null, 0.05956, null, 0.67836, null, 0.4884, null, 150, null, "B", null, "usable mapping rate", "bgi", "bgi", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2021-11-17", "Larval", "Larval", "Undetermined", "Undetermined"], [66969, "SRR16965148", "SRX13156587", "SRS11088491", "SRP346547", "PRJNA780808", "transcriptomics of carbendazim and enrofloxacin on zebrafish embryos", "PRJNA780808", "Other", "reveal a interaction between ENF and CAR on metabolic regulation during development", null, null, null, null, "3con", null, "strain:Tu|isolate:N/A|breed:N/A|cultivar:N/A|ecotype:N/A|age:3 96hpf|dev stage:larvae|sex:male and female|tissue:larvae|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio :control group", "con 3", "con 3", "96hpf zebrafish larvae control group", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "SINGLE", "BGISEQ", "BGISEQ-500", null, "SRP346547", null, null, "con_3_2.fq.gz", "fastq", 3359054500.0, 33590545.0, "con 3 2.fq.gz", "0:100 1:0", "A:914533337;C:755914202;G:773676246;T:914930715;N:0", 100, 0, null, null, 914533337, 755914202, 773676246, 914930715, 0, "SRX13156587", "SRS11088491", "SRA1329868", "China Agricultural University|College of animal science and technology", "China Agricultural University", 1, 0.95076, null, 0.10478, null, 0.65646, null, 0.49594, null, 100, null, "B", null, "usable mapping rate", "bgi", "bgi", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2021-11-17", "Larval", "Larval", "Undetermined", "Undetermined"], [66970, "SRR16965149", "SRX13156586", "SRS11088489", "SRP346547", "PRJNA780808", "transcriptomics of carbendazim and enrofloxacin on zebrafish embryos", "PRJNA780808", "Other", "reveal a interaction between ENF and CAR on metabolic regulation during development", null, null, null, null, "3mix", null, "strain:Tu|isolate:N/A|breed:N/A|cultivar:N/A|ecotype:N/A|age:12 96hpf|dev stage:larvae|sex:male and female|tissue:larvae|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio :mixture group", "mix 3", "mix 3", "96hpf zebrafish larvae mixture group", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "SINGLE", "BGISEQ", "BGISEQ-500", null, "SRP346547", null, null, "mix2_3_1.fq.gz", "fastq", 3362094000.0, 33620940.0, "mix2 3 1.fq.gz", "0:100 1:0", "A:916124379;C:755910593;G:768246534;T:921812494;N:0", 100, 0, null, null, 916124379, 755910593, 768246534, 921812494, 0, "SRX13156586", "SRS11088489", "SRA1329868", "China Agricultural University|College of animal science and technology", "China Agricultural University", 1, 0.94894, null, 0.10416, null, 0.65342, null, 0.49475, null, 100, null, "B", null, "usable mapping rate", "bgi", "bgi", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2021-11-17", "Larval", "Larval", "Undetermined", "Undetermined"], [66972, "SRR16965151", "SRX13156584", "SRS11088488", "SRP346547", "PRJNA780808", "transcriptomics of carbendazim and enrofloxacin on zebrafish embryos", "PRJNA780808", "Other", "reveal a interaction between ENF and CAR on metabolic regulation during development", null, null, null, null, "2con", null, "strain:Tu|isolate:N/A|breed:N/A|cultivar:N/A|ecotype:N/A|age:2 96hpf|dev stage:larvae|sex:male and female|tissue:larvae|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio :control group", "con 2", "con 2", "96hpf zebrafish larvae control group", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "SINGLE", "BGISEQ", "BGISEQ-500", null, "SRP346547", null, "loader:fastq load.py", "con_2_2.fq", "fastq", 3324287550.0, 22161917.0, "con 2 2.fq.gz", "0:150", "A:848766265;C:812477645;G:798995610;T:864040426;N:7604", 150, null, null, null, 848766265, 812477645, 798995610, 864040426, 7604, "SRX13156584", "SRS11088488", "SRA1329868", "China Agricultural University|College of animal science and technology", "China Agricultural University", 1, 0.95546, null, 0.03524, null, 0.70027, null, 0.48649, null, 150, null, "B", null, "usable mapping rate", "bgi", "bgi", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2021-11-22", "Larval", "Larval", "Undetermined", "Undetermined"], [66973, "SRR16965152", "SRX13156583", "SRS11088487", "SRP346547", "PRJNA780808", "transcriptomics of carbendazim and enrofloxacin on zebrafish embryos", "PRJNA780808", "Other", "reveal a interaction between ENF and CAR on metabolic regulation during development", null, null, null, null, "1con", null, "strain:Tu|isolate:N/A|breed:N/A|cultivar:N/A|ecotype:N/A|age:1 96hpf|dev stage:larvae|sex:male and female|tissue:larvae|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio :control group", "con 1", "con 1", "96hpf zebrafish larvae control group", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "SINGLE", "BGISEQ", "BGISEQ-500", null, "SRP346547", null, "loader:fastq load.py", "con_1_1.fq", "fastq", 3330724500.0, 22204830.0, "con 1 1.fq.gz", "0:150", "A:896812508;C:762246067;G:774062043;T:897597946;N:5936", 150, null, null, null, 896812508, 762246067, 774062043, 897597946, 5936, "SRX13156583", "SRS11088487", "SRA1329868", "China Agricultural University|College of animal science and technology", "China Agricultural University", 1, 0.94926, null, 0.08711, null, 0.66789, null, 0.48628, null, 150, null, "B", null, "usable mapping rate", "bgi", "bgi", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2021-11-22", "Larval", "Larval", "Undetermined", "Undetermined"], [66975, "SRR16959539", "SRX13151073", "SRS11083379", "SRP346418", "PRJNA780940", "transcriptomics of difenoconazole and dimethomorph on zebrafish embryos", "PRJNA780940", "Other", "Difenoconazole DIF and dimethomorph DIM are widely used pesticides frequently detected together in environmental samples  so the deleterious effects of combined exposure warrant detailed examination. In this study  the individual and combined effects of DIM and DIF on conventional developmental parameters hatch rate  deformity rate  lethality and gene expression were measured in embryonic zebrafish. Both DIF and DIM interfered with normal zebrafish embryo development  and the most sensitive toxicity index for both was 96 hpf deformity rate BMDL10 values of 0.30 and 1.10 mg/L  respectively. The combination of DIF and DIM had mainly synergistic deleterious effects on 96 hpf deformity and mortality rates. Transcriptome analysis showed that these compounds markedly downregulated expression of mcm family genes  cdk1  and cdc20  thereby potentially disrupting DNA replication and cell cycle progression. Enhanced surveillance for this pesticide combination is recommended as simultaneous environmental exposure may be substantially more harmful than exposure to either compound alone.", null, null, null, null, "2dim", null, "strain:Tu|isolate:N/A|breed:N/A|cultivar:N/A|ecotype:N/A|age:8 96h|dev stage:larvae|sex:male and female|tissue:larvae|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio : dimethomorph group", "dim 2", "dim 2", "The final library was amplified with phi29 to make DNA nanoballs DNB  with more than 300 copies of one molecular.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "SINGLE", "BGISEQ", "BGISEQ-500", null, "SRP346418", null, null, "dim_2_1.fq.gz", "fastq", 3344257500.0, 22295050.0, "dim 2 1.fq.gz", "0:150 1:0", "A:895700119;C:767415749;G:784994320;T:896143585;N:3727", 150, 0, null, null, 895700119, 767415749, 784994320, 896143585, 3727, "SRX13151073", "SRS11083379", "SRA1330365", "China Agricultural University|College of animal science and technology", "China Agricultural University", 1, 0.9497, null, 0.08418, null, 0.66352, null, 0.49341, null, 150, null, "B", null, "usable mapping rate", "bgi", "bgi", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2021-11-16", "Larval", "Larval", "Undetermined", "Undetermined"], [66976, "SRR16959540", "SRX13151072", "SRS11083378", "SRP346418", "PRJNA780940", "transcriptomics of difenoconazole and dimethomorph on zebrafish embryos", "PRJNA780940", "Other", "Difenoconazole DIF and dimethomorph DIM are widely used pesticides frequently detected together in environmental samples  so the deleterious effects of combined exposure warrant detailed examination. In this study  the individual and combined effects of DIM and DIF on conventional developmental parameters hatch rate  deformity rate  lethality and gene expression were measured in embryonic zebrafish. Both DIF and DIM interfered with normal zebrafish embryo development  and the most sensitive toxicity index for both was 96 hpf deformity rate BMDL10 values of 0.30 and 1.10 mg/L  respectively. The combination of DIF and DIM had mainly synergistic deleterious effects on 96 hpf deformity and mortality rates. Transcriptome analysis showed that these compounds markedly downregulated expression of mcm family genes  cdk1  and cdc20  thereby potentially disrupting DNA replication and cell cycle progression. Enhanced surveillance for this pesticide combination is recommended as simultaneous environmental exposure may be substantially more harmful than exposure to either compound alone.", null, null, null, null, "1dim", null, "strain:Tu|isolate:N/A|breed:N/A|cultivar:N/A|ecotype:N/A|age:7 96h|dev stage:larvae|sex:male and female|tissue:larvae|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio : dimethomorph group", "dim 1", "dim 1", "The final library was amplified with phi29 to make DNA nanoballs DNB  with more than 300 copies of one molecular.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "SINGLE", "BGISEQ", "BGISEQ-500", null, "SRP346418", null, null, "dim_1_1.fq.gz", "fastq", 3318812250.0, 22125415.0, "dim 1 1.fq.gz", "0:150 1:0", "A:908481293;C:742787936;G:755930542;T:911608821;N:3658", 150, 0, null, null, 908481293, 742787936, 755930542, 911608821, 3658, "SRX13151072", "SRS11083378", "SRA1330365", "China Agricultural University|College of animal science and technology", "China Agricultural University", 1, 0.94211, null, 0.11824, null, 0.65344, null, 0.47716, null, 150, null, "B", null, "usable mapping rate", "bgi", "bgi", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2021-11-16", "Larval", "Larval", "Undetermined", "Undetermined"], [66977, "SRR16959541", "SRX13151071", "SRS11083377", "SRP346418", "PRJNA780940", "transcriptomics of difenoconazole and dimethomorph on zebrafish embryos", "PRJNA780940", "Other", "Difenoconazole DIF and dimethomorph DIM are widely used pesticides frequently detected together in environmental samples  so the deleterious effects of combined exposure warrant detailed examination. In this study  the individual and combined effects of DIM and DIF on conventional developmental parameters hatch rate  deformity rate  lethality and gene expression were measured in embryonic zebrafish. Both DIF and DIM interfered with normal zebrafish embryo development  and the most sensitive toxicity index for both was 96 hpf deformity rate BMDL10 values of 0.30 and 1.10 mg/L  respectively. The combination of DIF and DIM had mainly synergistic deleterious effects on 96 hpf deformity and mortality rates. Transcriptome analysis showed that these compounds markedly downregulated expression of mcm family genes  cdk1  and cdc20  thereby potentially disrupting DNA replication and cell cycle progression. Enhanced surveillance for this pesticide combination is recommended as simultaneous environmental exposure may be substantially more harmful than exposure to either compound alone.", null, null, null, null, "3dif", null, "strain:Tu|isolate:N/A|breed:N/A|cultivar:N/A|ecotype:N/A|age:6 96h|dev stage:larvae|sex:male and female|tissue:larvae|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio : difenoconazole group", "dif 3", "dif 3", "The final library was amplified with phi29 to make DNA nanoballs DNB  with more than 300 copies of one molecular.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "SINGLE", "BGISEQ", "BGISEQ-500", null, "SRP346418", null, null, "dif_3_1.fq.gz", "fastq", 3318453000.0, 22123020.0, "dif 3 1.fq.gz", "0:150 1:0", "A:893975607;C:758489621;G:772391705;T:893592335;N:3732", 150, 0, null, null, 893975607, 758489621, 772391705, 893592335, 3732, "SRX13151071", "SRS11083377", "SRA1330365", "China Agricultural University|College of animal science and technology", "China Agricultural University", 1, 0.94838, null, 0.08393, null, 0.66543, null, 0.4818, null, 150, null, "B", null, "usable mapping rate", "bgi", "bgi", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2021-11-16", "Larval", "Larval", "Undetermined", "Undetermined"], [66978, "SRR16959542", "SRX13151070", "SRS11083376", "SRP346418", "PRJNA780940", "transcriptomics of difenoconazole and dimethomorph on zebrafish embryos", "PRJNA780940", "Other", "Difenoconazole DIF and dimethomorph DIM are widely used pesticides frequently detected together in environmental samples  so the deleterious effects of combined exposure warrant detailed examination. In this study  the individual and combined effects of DIM and DIF on conventional developmental parameters hatch rate  deformity rate  lethality and gene expression were measured in embryonic zebrafish. Both DIF and DIM interfered with normal zebrafish embryo development  and the most sensitive toxicity index for both was 96 hpf deformity rate BMDL10 values of 0.30 and 1.10 mg/L  respectively. The combination of DIF and DIM had mainly synergistic deleterious effects on 96 hpf deformity and mortality rates. Transcriptome analysis showed that these compounds markedly downregulated expression of mcm family genes  cdk1  and cdc20  thereby potentially disrupting DNA replication and cell cycle progression. Enhanced surveillance for this pesticide combination is recommended as simultaneous environmental exposure may be substantially more harmful than exposure to either compound alone.", null, null, null, null, "2dif", null, "strain:Tu|isolate:N/A|breed:N/A|cultivar:N/A|ecotype:N/A|age:5 96h|dev stage:larvae|sex:male and female|tissue:larvae|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio : difenoconazole group", "dif 2", "dif 2", "The final library was amplified with phi29 to make DNA nanoballs DNB  with more than 300 copies of one molecular.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "SINGLE", "BGISEQ", "BGISEQ-500", null, "SRP346418", null, null, "dif_2_2.fq.gz", "fastq", 3304972350.0, 22033149.0, "dif 2 2.fq.gz", "0:0 1:150", "A:890456734;C:764917766;G:744757536;T:904833787;N:6527", 0, 150, null, null, 890456734, 764917766, 744757536, 904833787, 6527, "SRX13151070", "SRS11083376", "SRA1330365", "China Agricultural University|College of animal science and technology", "China Agricultural University", 1, 0.93514, null, 0.107, null, 0.6633, null, 0.47599, null, 150, null, "B", null, "usable mapping rate", "bgi", "bgi", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2021-11-16", "Larval", "Larval", "Undetermined", "Undetermined"], [66979, "SRR16959543", "SRX13151069", "SRS11083375", "SRP346418", "PRJNA780940", "transcriptomics of difenoconazole and dimethomorph on zebrafish embryos", "PRJNA780940", "Other", "Difenoconazole DIF and dimethomorph DIM are widely used pesticides frequently detected together in environmental samples  so the deleterious effects of combined exposure warrant detailed examination. In this study  the individual and combined effects of DIM and DIF on conventional developmental parameters hatch rate  deformity rate  lethality and gene expression were measured in embryonic zebrafish. Both DIF and DIM interfered with normal zebrafish embryo development  and the most sensitive toxicity index for both was 96 hpf deformity rate BMDL10 values of 0.30 and 1.10 mg/L  respectively. The combination of DIF and DIM had mainly synergistic deleterious effects on 96 hpf deformity and mortality rates. Transcriptome analysis showed that these compounds markedly downregulated expression of mcm family genes  cdk1  and cdc20  thereby potentially disrupting DNA replication and cell cycle progression. Enhanced surveillance for this pesticide combination is recommended as simultaneous environmental exposure may be substantially more harmful than exposure to either compound alone.", null, null, null, null, "1dif", null, "strain:Tu|isolate:N/A|breed:N/A|cultivar:N/A|ecotype:N/A|age:4 96h|dev stage:larvae|sex:male and female|tissue:larvae|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio : difenoconazole group", "dif 1", "dif 1", "The final library was amplified with phi29 to make DNA nanoballs DNB  with more than 300 copies of one molecular.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "SINGLE", "BGISEQ", "BGISEQ-500", null, "SRP346418", null, null, "dif_1_1.fq.gz", "fastq", 3295443900.0, 21969626.0, "dif 1 1.fq.gz", "0:150 1:0", "A:887936493;C:751636494;G:765508757;T:890358629;N:3527", 150, 0, null, null, 887936493, 751636494, 765508757, 890358629, 3527, "SRX13151069", "SRS11083375", "SRA1330365", "China Agricultural University|College of animal science and technology", "China Agricultural University", 1, 0.94623, null, 0.09138, null, 0.66478, null, 0.4828, null, 150, null, "B", null, "usable mapping rate", "bgi", "bgi", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2021-11-16", "Larval", "Larval", "Undetermined", "Undetermined"], [66980, "SRR16959544", "SRX13151068", "SRS11083374", "SRP346418", "PRJNA780940", "transcriptomics of difenoconazole and dimethomorph on zebrafish embryos", "PRJNA780940", "Other", "Difenoconazole DIF and dimethomorph DIM are widely used pesticides frequently detected together in environmental samples  so the deleterious effects of combined exposure warrant detailed examination. In this study  the individual and combined effects of DIM and DIF on conventional developmental parameters hatch rate  deformity rate  lethality and gene expression were measured in embryonic zebrafish. Both DIF and DIM interfered with normal zebrafish embryo development  and the most sensitive toxicity index for both was 96 hpf deformity rate BMDL10 values of 0.30 and 1.10 mg/L  respectively. The combination of DIF and DIM had mainly synergistic deleterious effects on 96 hpf deformity and mortality rates. Transcriptome analysis showed that these compounds markedly downregulated expression of mcm family genes  cdk1  and cdc20  thereby potentially disrupting DNA replication and cell cycle progression. Enhanced surveillance for this pesticide combination is recommended as simultaneous environmental exposure may be substantially more harmful than exposure to either compound alone.", null, null, null, null, "3+con", null, "strain:Tu|isolate:N/A|breed:N/A|cultivar:N/A|ecotype:N/A|age:3 96h|dev stage:larvae|sex:male and female|tissue:larvae|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio : control group", "con 3", "con 3", "The final library was amplified with phi29 to make DNA nanoballs DNB  with more than 300 copies of one molecular.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "SINGLE", "BGISEQ", "BGISEQ-500", null, "SRP346418", null, null, "con_3_1.fq.gz", "fastq", 3306406800.0, 22042712.0, "con 3 1.fq.gz", "0:150 1:0", "A:872740236;C:773482184;G:788334419;T:871846539;N:3422", 150, 0, null, null, 872740236, 773482184, 788334419, 871846539, 3422, "SRX13151068", "SRS11083374", "SRA1330365", "China Agricultural University|College of animal science and technology", "China Agricultural University", 1, 0.95458, null, 0.06103, null, 0.67409, null, 0.48439, null, 150, null, "B", null, "usable mapping rate", "bgi", "bgi", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2021-11-16", "Larval", "Larval", "Undetermined", "Undetermined"], [66982, "SRR16959546", "SRX13151066", "SRS11083372", "SRP346418", "PRJNA780940", "transcriptomics of difenoconazole and dimethomorph on zebrafish embryos", "PRJNA780940", "Other", "Difenoconazole DIF and dimethomorph DIM are widely used pesticides frequently detected together in environmental samples  so the deleterious effects of combined exposure warrant detailed examination. In this study  the individual and combined effects of DIM and DIF on conventional developmental parameters hatch rate  deformity rate  lethality and gene expression were measured in embryonic zebrafish. Both DIF and DIM interfered with normal zebrafish embryo development  and the most sensitive toxicity index for both was 96 hpf deformity rate BMDL10 values of 0.30 and 1.10 mg/L  respectively. The combination of DIF and DIM had mainly synergistic deleterious effects on 96 hpf deformity and mortality rates. Transcriptome analysis showed that these compounds markedly downregulated expression of mcm family genes  cdk1  and cdc20  thereby potentially disrupting DNA replication and cell cycle progression. Enhanced surveillance for this pesticide combination is recommended as simultaneous environmental exposure may be substantially more harmful than exposure to either compound alone.", null, null, null, null, "2+con", null, "strain:Tu|isolate:N/A|breed:N/A|cultivar:N/A|ecotype:N/A|age:2 96h|dev stage:larvae|sex:male and female|tissue:larvae|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio : control group", "con 2", "con 2", "The final library was amplified with phi29 to make DNA nanoballs DNB  with more than 300 copies of one molecular.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "SINGLE", "BGISEQ", "BGISEQ-500", null, "SRP346418", null, null, "con_2_2.fq.gz", "fastq", 3324287550.0, 22161917.0, "con 2 2.fq.gz", "0:0 1:150", "A:848766265;C:812477645;G:798995610;T:864040426;N:7604", 0, 150, null, null, 848766265, 812477645, 798995610, 864040426, 7604, "SRX13151066", "SRS11083372", "SRA1330365", "China Agricultural University|College of animal science and technology", "China Agricultural University", 1, 0.95548, null, 0.03524, null, 0.70021, null, 0.4867, null, 150, null, "B", null, "usable mapping rate", "bgi", "bgi", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2021-11-16", "Larval", "Larval", "Undetermined", "Undetermined"], [66983, "SRR16959547", "SRX13151065", "SRS11083371", "SRP346418", "PRJNA780940", "transcriptomics of difenoconazole and dimethomorph on zebrafish embryos", "PRJNA780940", "Other", "Difenoconazole DIF and dimethomorph DIM are widely used pesticides frequently detected together in environmental samples  so the deleterious effects of combined exposure warrant detailed examination. In this study  the individual and combined effects of DIM and DIF on conventional developmental parameters hatch rate  deformity rate  lethality and gene expression were measured in embryonic zebrafish. Both DIF and DIM interfered with normal zebrafish embryo development  and the most sensitive toxicity index for both was 96 hpf deformity rate BMDL10 values of 0.30 and 1.10 mg/L  respectively. The combination of DIF and DIM had mainly synergistic deleterious effects on 96 hpf deformity and mortality rates. Transcriptome analysis showed that these compounds markedly downregulated expression of mcm family genes  cdk1  and cdc20  thereby potentially disrupting DNA replication and cell cycle progression. Enhanced surveillance for this pesticide combination is recommended as simultaneous environmental exposure may be substantially more harmful than exposure to either compound alone.", null, null, null, null, "1+con", null, "strain:Tu|isolate:N/A|breed:N/A|cultivar:N/A|ecotype:N/A|age:1 96h|dev stage:larvae|sex:male and female|tissue:larvae|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio : control group", "con 1", "con 1", "The final library was amplified with phi29 to make DNA nanoballs DNB  with more than 300 copies of one molecular.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "SINGLE", "BGISEQ", "BGISEQ-500", null, "SRP346418", null, null, "con_1_1.fq.gz", "fastq", 3330724500.0, 22204830.0, "con 1 1.fq.gz", "0:150 1:0", "A:896812508;C:762246067;G:774062043;T:897597946;N:5936", 150, 0, null, null, 896812508, 762246067, 774062043, 897597946, 5936, "SRX13151065", "SRS11083371", "SRA1330365", "China Agricultural University|College of animal science and technology", "China Agricultural University", 1, 0.94925, null, 0.08711, null, 0.66772, null, 0.48635, null, 150, null, "B", null, "usable mapping rate", "bgi", "bgi", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2021-11-16", "Larval", "Larval", "Undetermined", "Undetermined"]], "truncated": false, "filtered_table_rows_count": 25, "expanded_columns": [], "expandable_columns": [], "columns": ["rowid", "run.accession", "experiment.accession", "sample.accession", "study.accession", "bioproject", "study.title", "study.alias", "study.type", "study.abstract", "study.attributes", "study.PMIDs", "sample.description", "sample.title", "sample.alias", "sample.centername", "sample.attributes", "GEOsample.title", "GEOsample.dataprocessing", "GEOsample.source", "GEOsample.treatmentprotocol", "GEOsample.extractprotocol", "GEOsample.growthprotocol", "GEOsample.characteristics", "GEOsample.accession", "experiment.title", 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