{"database": "metadata", "table": "run_metadata", "is_view": false, "human_description_en": "where experiment.library_layout = \"PAIRED\", technology = \"smartseq\" and tissue_curation = \"Tail\"", "rows": [[54735, "SRR10151942", "SRX6877449", "SRS5413231", "SRP222763", "PRJNA572587", "Characterization of T cells from the larval zebrafish tail via single cell RNAseq", "GSE137770", "Transcriptome Analysis", "We report single cell RNA sequencing of cells from the Tglck:GFP larval zebrafish tail  to identify cell types expressing the marker and to identify gene candidates related to T cell migration behaviors. We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. 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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s378", "GSM4087499", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s378", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s377", "GSM4087498", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s377", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s373", "GSM4087494", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s373", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s372", "GSM4087493", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s372", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s371", "GSM4087492", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s371", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s370", "GSM4087491", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s370", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s369", "GSM4087490", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s369", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s368", "GSM4087489", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s368", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s367", "GSM4087488", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s367", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s366", "GSM4087487", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s366", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s365", "GSM4087486", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s365", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s364", "GSM4087485", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s364", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s363", "GSM4087484", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s363", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s362", "GSM4087483", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s362", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s361", "GSM4087482", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s361", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s360", "GSM4087481", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s360", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s167", "GSM4087288", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s167", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s166", "GSM4087287", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s166", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s165", "GSM4087286", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s165", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s164", "GSM4087285", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s164", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s163", "GSM4087284", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s163", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification  and a Nextera based protocol was used for library preparation  as described in Schaum et al.  Nature 2018", null, "line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "GSM4087284", "GSM4087284: lckgfp s163; Danio rerio; RNA Seq", "GSM4087284", null, "1", "Lysis was performed as described in Schaum et al.  Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification  and a Nextera based protocol was used for library preparation  as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s162", "GSM4087283", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s162", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s161", "GSM4087282", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s161", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s160", "GSM4087281", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s160", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s159", "GSM4087280", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s159", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s158", "GSM4087279", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s158", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s157", "GSM4087278", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s157", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification  and a Nextera based protocol was used for library preparation  as described in Schaum et al.  Nature 2018", null, "line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "GSM4087278", "GSM4087278: lckgfp s157; Danio rerio; RNA Seq", "GSM4087278", null, "1", "Lysis was performed as described in Schaum et al.  Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification  and a Nextera based protocol was used for library preparation  as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s156", "GSM4087277", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s156", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification  and a Nextera based protocol was used for library preparation  as described in Schaum et al.  Nature 2018", null, "line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "GSM4087277", "GSM4087277: lckgfp s156; Danio rerio; RNA Seq", "GSM4087277", null, "1", "Lysis was performed as described in Schaum et al.  Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification  and a Nextera based protocol was used for library preparation  as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s155", "GSM4087276", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s155", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification  and a Nextera based protocol was used for library preparation  as described in Schaum et al.  Nature 2018", null, "line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "GSM4087276", "GSM4087276: lckgfp s155; Danio rerio; RNA Seq", "GSM4087276", null, "1", "Lysis was performed as described in Schaum et al.  Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification  and a Nextera based protocol was used for library preparation  as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s154", "GSM4087275", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s154", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification  and a Nextera based protocol was used for library preparation  as described in Schaum et al.  Nature 2018", null, "line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "GSM4087275", "GSM4087275: lckgfp s154; Danio rerio; RNA Seq", "GSM4087275", null, "1", "Lysis was performed as described in Schaum et al.  Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification  and a Nextera based protocol was used for library preparation  as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s153", "GSM4087274", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s153", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification  and a Nextera based protocol was used for library preparation  as described in Schaum et al.  Nature 2018", null, "line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "GSM4087274", "GSM4087274: lckgfp s153; Danio rerio; RNA Seq", "GSM4087274", null, "1", "Lysis was performed as described in Schaum et al.  Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification  and a Nextera based protocol was used for library preparation  as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s152", "GSM4087273", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s152", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s151", "GSM4087272", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s151", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification  and a Nextera based protocol was used for library preparation  as described in Schaum et al.  Nature 2018", null, "line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "GSM4087272", "GSM4087272: lckgfp s151; Danio rerio; RNA Seq", "GSM4087272", null, "1", "Lysis was performed as described in Schaum et al.  Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification  and a Nextera based protocol was used for library preparation  as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s150", "GSM4087271", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s150", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s149", "GSM4087270", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s149", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s148", "GSM4087269", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s148", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s147", "GSM4087268", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s147", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s146", "GSM4087267", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s146", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s145", "GSM4087266", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s145", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s144", "GSM4087265", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s144", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s263", "GSM4087384", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s263", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification  and a Nextera based protocol was used for library preparation  as described in Schaum et al.  Nature 2018", null, "line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "GSM4087384", "GSM4087384: lckgfp s263; Danio rerio; RNA Seq", "GSM4087384", null, "1", "Lysis was performed as described in Schaum et al.  Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification  and a Nextera based protocol was used for library preparation  as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s262", "GSM4087383", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s262", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s261", "GSM4087382", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s261", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s260", "GSM4087381", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s260", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s259", "GSM4087380", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s259", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification  and a Nextera based protocol was used for library preparation  as described in Schaum et al.  Nature 2018", null, "line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "GSM4087380", "GSM4087380: lckgfp s259; Danio rerio; RNA Seq", "GSM4087380", null, "1", "Lysis was performed as described in Schaum et al.  Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification  and a Nextera based protocol was used for library preparation  as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s258", "GSM4087379", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s258", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification  and a Nextera based protocol was used for library preparation  as described in Schaum et al.  Nature 2018", null, "line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "GSM4087379", "GSM4087379: lckgfp s258; Danio rerio; RNA Seq", "GSM4087379", null, "1", "Lysis was performed as described in Schaum et al.  Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification  and a Nextera based protocol was used for library preparation  as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s257", "GSM4087378", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s257", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification  and a Nextera based protocol was used for library preparation  as described in Schaum et al.  Nature 2018", null, "line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "GSM4087378", "GSM4087378: lckgfp s257; Danio rerio; RNA Seq", "GSM4087378", null, "1", "Lysis was performed as described in Schaum et al.  Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification  and a Nextera based protocol was used for library preparation  as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s256", "GSM4087377", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s256", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification  and a Nextera based protocol was used for library preparation  as described in Schaum et al.  Nature 2018", null, "line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "GSM4087377", "GSM4087377: lckgfp s256; Danio rerio; RNA Seq", "GSM4087377", null, "1", "Lysis was performed as described in Schaum et al.  Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification  and a Nextera based protocol was used for library preparation  as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s255", "GSM4087376", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s255", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s254", "GSM4087375", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s254", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s253", "GSM4087374", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s253", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s252", "GSM4087373", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s252", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s251", "GSM4087372", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s251", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s248", "GSM4087369", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s248", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s247", "GSM4087368", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s247", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s246", "GSM4087367", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s246", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s245", "GSM4087366", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s245", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s243", "GSM4087364", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s243", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s242", "GSM4087363", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s242", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s241", "GSM4087362", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s241", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s240", "GSM4087361", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s240", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s359", "GSM4087480", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s359", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s358", "GSM4087479", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s358", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s357", "GSM4087478", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s357", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s356", "GSM4087477", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s356", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s355", "GSM4087476", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s355", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s354", "GSM4087475", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s354", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s353", "GSM4087474", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s353", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s352", "GSM4087473", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s352", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s351", "GSM4087472", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s351", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s350", "GSM4087471", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s350", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s349", "GSM4087470", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s349", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s348", "GSM4087469", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s348", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s347", "GSM4087468", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s347", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s346", "GSM4087467", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s346", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s345", "GSM4087466", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s345", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification  and a Nextera based protocol was used for library preparation  as described in Schaum et al.  Nature 2018", null, "line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "GSM4087466", "GSM4087466: lckgfp s345; Danio rerio; RNA Seq", "GSM4087466", null, "1", "Lysis was performed as described in Schaum et al.  Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification  and a Nextera based protocol was used for library preparation  as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s344", "GSM4087465", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s344", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification  and a Nextera based protocol was used for library preparation  as described in Schaum et al.  Nature 2018", null, "line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "GSM4087465", "GSM4087465: lckgfp s344; Danio rerio; RNA Seq", "GSM4087465", null, "1", "Lysis was performed as described in Schaum et al.  Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification  and a Nextera based protocol was used for library preparation  as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s343", "GSM4087464", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s343", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification  and a Nextera based protocol was used for library preparation  as described in Schaum et al.  Nature 2018", null, "line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "GSM4087464", "GSM4087464: lckgfp s343; Danio rerio; RNA Seq", "GSM4087464", null, "1", "Lysis was performed as described in Schaum et al.  Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification  and a Nextera based protocol was used for library preparation  as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s342", "GSM4087463", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s342", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification  and a Nextera based protocol was used for library preparation  as described in Schaum et al.  Nature 2018", null, "line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "GSM4087463", "GSM4087463: lckgfp s342; Danio rerio; RNA Seq", "GSM4087463", null, "1", "Lysis was performed as described in Schaum et al.  Nature 2018 A Smart Seq2 based protocol was used for reverse transcription and cDNA amplification  and a Nextera based protocol was used for library preparation  as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s341", "GSM4087462", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s341", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s340", "GSM4087461", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s340", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s339", "GSM4087460", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s339", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  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We identified 330 putative T cells and 131 putative epithelial cells. Differential expression analysis between the two groups revealed genes related to actin cytoskeleton remodeling associated with the T cells  in addition to canonical T and ubiquitous immune cell markers. Overall design: Single cells n=461 isolated from FACS of Tglck:GFP Danio rerio FITC+ were analyzed.", null, "pubmed:32427565", null, "lckgfp s338", "GSM4087459", null, "source name:Danio rerio tail|line:Tglck:GFP|tissue:tail|devlopmental stage:15 dpf", "lckgfp s338", "STAR 2.5 Htseq 0.8.0 htseq count  m intersection nonempty   nonunique all Genome build: GRCz10 Supplementary files format and content: Raw counts of numbers of reads aligned to each feature for each cell  in tab separated format lckgfp counts.txt", "Danio rerio tail", "Liberase TL 100 ug/mL and manual trituration was used to dissociate tail tissue. Cells were FACS sorted FITC+", "Lysis was performed as described in Schaum et al.  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