{"database": "metadata", "table": "run_metadata", "is_view": false, "human_description_en": "where experiment.library_layout = \"PAIRED\", experiment.library_source = \"TRANSCRIPTOMIC\" and tissue_curation = \"Embryo Imprecise\"", "rows": [[8055, "ERR022484", "ERX008924", "ERS017427", "ERP000400", "PRJEB2333", "Sequencing the Zebrafish transcriptome form a range of tissues and developmental stages using the Illumina Genome Analyzer", "E-MTAB-434", "Other", null, null, null, null, "E MTAB 434:ZF 2cells", "SAMEA898400", "Wellcome Sanger Institute", "Alias:E MTAB 434:ZF 2cells|Broker name:ArrayExpress|Description:Protocols: Zebrafish embyos or tissues were collected from a Tuebingen strain incross and grown at 28 C. Collected samples were snap frozen on dry ice and stored at  70 C Total RNA was extracted using Trizol Reagent Invitrogen following the manufacturer's instructions. Pellets were resuspended RNase free 10 mM Tris pH 7.5 and the RNA was quantified using a NanoDrop ND 1000 Spectrophotometer Axon Instruments. Total RNA was made into an RNAseq Illumina library following the manufacturer's protocol including a DNase treatment between to 2 rounds of polyA pull down. The libraries have fragment size of 250 to 300 bp.|DevelopmentalStage:embryo|INSDC center alias:SC|INSDC center name:Wellcome Sanger Institute|INSDC first public:2011 03 10T17:55:05Z|INSDC last update:2018 03 08T15:25:14Z|INSDC status:public|SRA accession:ERS017427|Sample Name:ERS017427|Sex:mixed|StrainOrLine:Tuebingen|Title:ZF 2cells", null, null, null, null, null, null, null, null, "Illumina Genome Analyzer II paired end sequencing; Sequencing the Zebrafish transcriptome form a range of tissues and developmental stages using the Illumina Genome Analyzer", "E MTAB 434:sequencing of Zebrafish embryo 2cells", "RNA from Zebrafish embryo 2cells", "Sequencing the Zebrafish transcriptome form a range of tissues and developmental stages using the Illumina Genome Analyzer", "Zebrafish embyos or tissues were collected from a Tuebingen strain incross and grown at 28 C.   Collected samples were snap frozen on dry ice and stored at  70 C Total RNA was extracted using Trizol Reagent Invitrogen following the manufacturer's instructions. Pellets were resuspended RNase free 10 mM Tris pH 7.5 and the RNA was quantified using a NanoDrop ND 1000 Spectrophotometer Axon Instruments. Total RNA was made into an RNAseq Illumina library following the manufacturer's protocol including a DNase treatment between to 2 rounds of polyA pull down.   The libraries have fragment size of 250 to 300 bp.", "Experimental Factor: DEVELPOMENTAL STAGE:embryo|Experimental Factor: ORGANISM PART:cell", "FL-cDNA", "TRANSCRIPTOMIC", "unspecified", "PAIRED", "ILLUMINA", "Illumina Genome Analyzer II", null, "ERP000400", "Illumina Genome Analyzer II paired end sequencing; Sequencing the Zebrafish transcriptome form a range of tissues and developmental stages using the Illumina Genome Analyzer", "ENA FIRST PUBLIC:2011 03 10|ENA LAST UPDATE:2018 11 16", "4946_5.srf", "srf", 3947547008.0, 25970704.0, "E MTAB 434:4946 5.srf", "0:76 1:76", "A:1069302461;C:914233601;G:902631356;T:1055986090;N:5393500", 76, 76, null, null, 1069302461, 914233601, 902631356, 1055986090, 5393500, "ERX008924", "ERS017427", "ERA015179", "SC|Wellcome Trust Sanger Institute", "SC|Wellcome Trust Sanger Institute", 2, 0.93356, 0.93346, 0.03988, 0.04022, 0.79135, 0.79198, 0.48864, 0.48464, 76, 76, "B", "B", "biological fallback assumption", "illumina", "early_illumina", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "United Kingdom", "2011-03-10", "Cleavage", "Embryo", "Embryo Imprecise", "All anatomical structures"], [9419, "ERR273825", "ERX248101", "ERS092357", "ERP001559", "PRJEB3118", "Zebrafish transcript profiling", "Zebrafish_transcript_profiling-sc-2012-06-28T15:47:32Z-82", "Transcriptome Analysis", "Paired end sequence data from the Illumina Genome Analyzer was prepared from normal and mutant zebrafish embryos for transcript profiling.   This includes pilot studies for transcript indexing within the sequence reads.", null, null, null, null, "SAMEA1888984", "SC", "ArrayExpress Sex:mixed|ArrayExpress Species:Danio rerio|ENA FIRST PUBLIC:2013 05 13T11:13:14Z|ENA LAST UPDATE:2018 03 08T15:36:21Z|External Id:SAMEA1888984|INSDC center name:SC|INSDC first public:2013 05 13T11:13:14Z|INSDC last update:2018 03 08T15:36:21Z|INSDC status:public|Submitter Id:hu2117 mutant vs wild type sc 2012 02 06T13:11:11Z 1107268|common name:zebrafish|sample description:3 prime end enriched mRNA from 3 morphological mutant hu2117 embryo samples and 3 matched sibling wild type samples. A 6 base indexing sequence is bases 5 to 10 of read 1 followed by polyT.|sample name:hu2117 mutant vs wild type sc 2012 02 06T13:11:11Z 1107268|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "1", "SC EXP 6316 8", "2387558", "Illumina sequencing of library 2387558  constructed from sample accession ERS092357 for study accession ERP001559.", "qPCR only", null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina HiSeq 2000", null, "ERP001559", "Illumina HiSeq 2000 paired end sequencing", "ENA FIRST PUBLIC:2013 05 13|ENA LAST UPDATE:2018 11 16", "6316_8.bam", "bam", 12715227300.0, 84768182.0, "SC RUN 6316 8", "0:75 1:75", "A:3986328155;C:1983554902;G:2466211000;T:4277430579;N:1702664", 75, 75, null, null, 3986328155, 1983554902, 2466211000, 4277430579, 1702664, "ERX248101", "ERS092357", "ERA212579", "SC", "Wellcome Sanger Institute", 2, 0.06657, 0.60346, 0.04569, 0.19203, 0.9767, 0.82014, 0.52373, 0.4048, 75, 75, "T", "B", "mate1 technical by mapping diff", "illumina", "hiseq_era", "3prime", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United Kingdom", "2013-05-13", "Undetermined", "Embryo", "Embryo Imprecise", "All anatomical structures"], [9420, "ERR273824", "ERX248100", "ERS092356", "ERP001559", "PRJEB3118", "Zebrafish transcript profiling", "Zebrafish_transcript_profiling-sc-2012-06-28T15:47:32Z-82", "Transcriptome Analysis", "Paired end sequence data from the Illumina Genome Analyzer was prepared from normal and mutant zebrafish embryos for transcript profiling.   This includes pilot studies for transcript indexing within the sequence reads.", null, null, null, null, "SAMEA1889000", "SC", "ArrayExpress Sex:mixed|ArrayExpress Species:Danio rerio|ENA FIRST PUBLIC:2013 05 13T11:13:13Z|ENA LAST UPDATE:2018 03 08T15:36:25Z|External Id:SAMEA1889000|INSDC center name:SC|INSDC first public:2013 05 13T11:13:13Z|INSDC last update:2018 03 08T15:36:25Z|INSDC status:public|Submitter Id:e48 mutant vs wild type sc 2012 02 06T13:11:09Z 1107267|common name:zebrafish|sample description:3 prime end enriched mRNA from 3 morphological mutant e48 embryo samples and 3 matched sibling wild type samples. A 6 base indexing sequence is bases 5 to 10 of read 1 followed by polyT.|sample name:e48 mutant vs wild type sc 2012 02 06T13:11:09Z 1107267|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "1", "SC EXP 6316 7", "2387557", "Illumina sequencing of library 2387557  constructed from sample accession ERS092356 for study accession ERP001559.", "qPCR only", null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina HiSeq 2000", null, "ERP001559", "Illumina HiSeq 2000 paired end sequencing", "ENA FIRST PUBLIC:2013 05 13|ENA LAST UPDATE:2018 11 16", "6316_7.bam", "bam", 17475932100.0, 116506214.0, "SC RUN 6316 7", "0:75 1:75", "A:5548228936;C:2693024343;G:3365940794;T:5867204994;N:1533033", 75, 75, null, null, 5548228936, 2693024343, 3365940794, 5867204994, 1533033, "ERX248100", "ERS092356", "ERA212579", "SC", "Wellcome Sanger Institute", 2, 0.05939, 0.59457, 0.04089, 0.19671, 0.98068, 0.838, 0.46304, 0.62741, 75, 75, "T", "B", "mate1 technical by mapping diff", "illumina", "hiseq_era", "3prime", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United Kingdom", "2013-05-13", "Undetermined", "Embryo", "Embryo Imprecise", "All anatomical structures"], [9421, "ERR273823", "ERX248099", "ERS092354", "ERP001559", "PRJEB3118", "Zebrafish transcript profiling", "Zebrafish_transcript_profiling-sc-2012-06-28T15:47:32Z-82", "Transcriptome Analysis", "Paired end sequence data from the Illumina Genome Analyzer was prepared from normal and mutant zebrafish embryos for transcript profiling.   This includes pilot studies for transcript indexing within the sequence reads.", null, null, null, null, "SAMEA1889008", "SC", "ArrayExpress Sex:mixed|ArrayExpress Species:Danio rerio|ENA FIRST PUBLIC:2013 05 13T11:13:13Z|ENA LAST UPDATE:2018 03 08T15:36:21Z|External Id:SAMEA1889008|INSDC center name:SC|INSDC first public:2013 05 13T11:13:13Z|INSDC last update:2018 03 08T15:36:21Z|INSDC status:public|Submitter Id:hu3332 mutant vs wild type sc 2012 02 06T13:11:07Z 1107265|common name:zebrafish|sample description:3 prime end enriched mRNA from 3 morphological mutant hu3332 embryo samples and 3 matched sibling wild type samples. A 6 base indexing sequence is bases 5 to 10 of read 1 followed by polyT.|sample name:hu3332 mutant vs wild type sc 2012 02 06T13:11:07Z 1107265|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "1", "SC EXP 6316 5", "2387555", "Illumina sequencing of library 2387555  constructed from sample accession ERS092354 for study accession ERP001559.", "qPCR only", null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina HiSeq 2000", null, "ERP001559", "Illumina HiSeq 2000 paired end sequencing", "ENA FIRST PUBLIC:2013 05 13|ENA LAST UPDATE:2018 11 16", "6316_5.bam", "bam", 13995917850.0, 93306119.0, "SC RUN 6316 5", "0:75 1:75", "A:4367273029;C:2176748810;G:2714026832;T:4736121060;N:1748119", 75, 75, null, null, 4367273029, 2176748810, 2714026832, 4736121060, 1748119, "ERX248099", "ERS092354", "ERA212579", "SC", "Wellcome Sanger Institute", 2, 0.06401, 0.62019, 0.04224, 0.18728, 0.97723, 0.81071, 0.461, 0.55379, 75, 75, "T", "B", "mate1 technical by mapping diff", "illumina", "hiseq_era", "3prime", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United Kingdom", "2013-05-13", "Undetermined", "Embryo", "Embryo Imprecise", "All anatomical structures"], [9422, "ERR273822", "ERX248098", "ERS092063", "ERP001559", "PRJEB3118", "Zebrafish transcript profiling", "Zebrafish_transcript_profiling-sc-2012-06-28T15:47:32Z-82", "Transcriptome Analysis", "Paired end sequence data from the Illumina Genome Analyzer was prepared from normal and mutant zebrafish embryos for transcript profiling.   This includes pilot studies for transcript indexing within the sequence reads.", null, null, null, null, "SAMEA1888996", "SC", "ArrayExpress Species:Danio rerio|ENA FIRST PUBLIC:2013 05 13T11:13:13Z|ENA LAST UPDATE:2018 03 08T15:36:13Z|External Id:SAMEA1888996|INSDC center name:SC|INSDC first public:2013 05 13T11:13:13Z|INSDC last update:2018 03 08T15:36:13Z|INSDC status:public|Submitter Id:sa0058 mutant vs wild type sc 2012 02 06T13:05:07Z 265521|common name:zebrafish|sample description:33 prime end enriched mRNA from 3 morphological mutant sa0058 embryo samples and 3 matched sibling wild type samples. A 6 base indexing sequence is bases 5 to 10 of read 1 followed by polyT.|sample name:sa0058 mutant vs wild type sc 2012 02 06T13:05:07Z 265521|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "1", "SC EXP 5287 7", "449230", "Illumina sequencing of library 449230  constructed from sample accession ERS092063 for study accession ERP001559.", "qPCR only", null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina Genome Analyzer II", null, "ERP001559", "Illumina Genome Analyzer II paired end sequencing", "ENA FIRST PUBLIC:2013 05 13|ENA LAST UPDATE:2018 11 16", "5287_7.bam", "bam", 2216402460.0, 20522245.0, "SC RUN 5287 7", "0:54 1:54", "A:646882787;C:346392353;G:394060191;T:827745173;N:1321956", 54, 54, null, null, 646882787, 346392353, 394060191, 827745173, 1321956, "ERX248098", "ERS092063", "ERA212579", "SC", "Wellcome Sanger Institute", 2, 0.02565, 0.72009, 0.01691, 0.21429, 0.98725, 0.78944, 0.62084, 0.61278, 54, 54, "T", "B", "mate1 technical by mapping diff", "illumina", "early_illumina", "3prime", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United Kingdom", "2013-05-13", "Undetermined", "Embryo", "Embryo Imprecise", "All anatomical structures"], [9892, "ERR4172795", "ERX4136409", "ERS4580819", "ERP121885", "PRJEB38455", "RNA seq of sfpq /  zebrafish embryos and siblings at 24 hpf", "E-MTAB-9113", "Transcriptome Analysis", "RNA was extracted from whole zebrafish embryos at 24 hpf to examine changes in gene expression and splicing in sfpq null mutants", "ENA FIRST PUBLIC:2021 01 27|ENA LAST UPDATE:2020 05 22", null, "Protocols: Embryos were collected from in crosses of sfpq+/  adult zebrafish RNA was extracted using the RNEasy Mini Kit Qiagen Library constructed with ribodepletion", "sibling 3", "SAMEA6853229", "Centre for Developmental Neurobiology King's College London", "ENA FIRST PUBLIC:2020 12 01T04:08:08Z|ENA LAST UPDATE:2020 05 22T17:13:15Z|External Id:SAMEA6853229|INSDC center name:Centre for Developmental Neurobiology King's College London|INSDC first public:2020 12 01T04:08:08Z|INSDC last update:2020 05 22T17:13:15Z|INSDC status:public|Submitter Id:E MTAB 9113:sibling 3|age:24|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo|genotype:wild type genotype|sample name:E MTAB 9113:sibling 3|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Illumina HiSeq 2500 paired end sequencing; RNA seq of sfpq /  zebrafish embryos and siblings at 24 hpf", "E MTAB 9113:sibling 3 p", "sibling 3 p", "RNA seq of sfpq /  zebrafish embryos and siblings at 24 hpf", "Embryos were collected from in crosses of sfpq+/  adult zebrafish RNA was extracted using the RNEasy Mini Kit Qiagen Library constructed with ribodepletion", "Experimental Factor: genotype:wild type genotype", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "ERP121885", "Illumina HiSeq 2500 paired end sequencing; RNA seq of sfpq /  zebrafish embryos and siblings at 24 hpf", "ENA FIRST PUBLIC:2021 01 27|ENA LAST UPDATE:2020 06 19", "UCLGNS1141-gfp-sibs-3_S6_R1.fastq.gz UCLGNS1141-gfp-sibs-3_S6_R2.fastq.gz", "fastq fastq", null, null, "E MTAB 9113:UCLGNS1141 gfp sibs 3 S6 R", "0:81 1:81", "A:1144578958;C:1113109875;G:1138809559;T:1117038434;N:213932", 81, 81, null, null, 1144578958, 1113109875, 1138809559, 1117038434, 213932, "ERX4136409", "ERS4580819", "ERA2625401", "Centre for Developmental Neurobiology King", "Centre for Developmental Neurobiology King", 2, 0.96684, 0.96492, 0.03244, 0.0317, 0.71236, 0.71514, 0.45871, 0.46189, 81, 81, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "United Kingdom", "2020-05-22", "Multi-stage", "Multi-stage", "Embryo Imprecise", "All anatomical structures"], [9893, "ERR4172794", "ERX4136408", "ERS4580818", "ERP121885", "PRJEB38455", "RNA seq of sfpq /  zebrafish embryos and siblings at 24 hpf", "E-MTAB-9113", "Transcriptome Analysis", "RNA was extracted from whole zebrafish embryos at 24 hpf to examine changes in gene expression and splicing in sfpq null mutants", "ENA FIRST PUBLIC:2021 01 27|ENA LAST UPDATE:2020 05 22", null, "Protocols: Embryos were collected from in crosses of sfpq+/  adult zebrafish RNA was extracted using the RNEasy Mini Kit Qiagen Library constructed with ribodepletion", "sibling 2", "SAMEA6853228", "Centre for Developmental Neurobiology King's College London", "ENA FIRST PUBLIC:2020 12 01T04:08:08Z|ENA LAST UPDATE:2020 05 22T17:13:15Z|External Id:SAMEA6853228|INSDC center name:Centre for Developmental Neurobiology King's College London|INSDC first public:2020 12 01T04:08:08Z|INSDC last update:2020 05 22T17:13:15Z|INSDC status:public|Submitter Id:E MTAB 9113:sibling 2|age:24|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo|genotype:wild type genotype|sample name:E MTAB 9113:sibling 2|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Illumina HiSeq 2500 paired end sequencing; RNA seq of sfpq /  zebrafish embryos and siblings at 24 hpf", "E MTAB 9113:sibling 2 p", "sibling 2 p", "RNA seq of sfpq /  zebrafish embryos and siblings at 24 hpf", "Embryos were collected from in crosses of sfpq+/  adult zebrafish RNA was extracted using the RNEasy Mini Kit Qiagen Library constructed with ribodepletion", "Experimental Factor: genotype:wild type genotype", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "ERP121885", "Illumina HiSeq 2500 paired end sequencing; RNA seq of sfpq /  zebrafish embryos and siblings at 24 hpf", "ENA FIRST PUBLIC:2021 01 27|ENA LAST UPDATE:2020 06 19", "UCLGNS1141-gfp-sibs-2_S4_R1.fastq.gz UCLGNS1141-gfp-sibs-2_S4_R2.fastq.gz", "fastq fastq", null, null, "E MTAB 9113:UCLGNS1141 gfp sibs 2 S4 R", "0:81 1:81", "A:1056280368;C:1036355469;G:1045429486;T:1035773622;N:189389", 81, 81, null, null, 1056280368, 1036355469, 1045429486, 1035773622, 189389, "ERX4136408", "ERS4580818", "ERA2625401", "Centre for Developmental Neurobiology King", "Centre for Developmental Neurobiology King", 2, 0.95511, 0.95696, 0.02941, 0.02903, 0.71492, 0.71628, 0.45765, 0.46537, 81, 81, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "United Kingdom", "2020-05-22", "Multi-stage", "Multi-stage", "Embryo Imprecise", "All anatomical structures"], [9894, "ERR4172793", "ERX4136407", "ERS4580817", "ERP121885", "PRJEB38455", "RNA seq of sfpq /  zebrafish embryos and siblings at 24 hpf", "E-MTAB-9113", "Transcriptome Analysis", "RNA was extracted from whole zebrafish embryos at 24 hpf to examine changes in gene expression and splicing in sfpq null mutants", "ENA FIRST PUBLIC:2021 01 27|ENA LAST UPDATE:2020 05 22", null, "Protocols: Embryos were collected from in crosses of sfpq+/  adult zebrafish RNA was extracted using the RNEasy Mini Kit Qiagen Library constructed with ribodepletion", "sibling 1", "SAMEA6853227", "Centre for Developmental Neurobiology King's College London", "ENA FIRST PUBLIC:2020 12 01T04:08:08Z|ENA LAST UPDATE:2020 05 22T17:13:15Z|External Id:SAMEA6853227|INSDC center name:Centre for Developmental Neurobiology King's College London|INSDC first public:2020 12 01T04:08:08Z|INSDC last update:2020 05 22T17:13:15Z|INSDC status:public|Submitter Id:E MTAB 9113:sibling 1|age:24|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo|genotype:wild type genotype|sample name:E MTAB 9113:sibling 1|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Illumina HiSeq 2500 paired end sequencing; RNA seq of sfpq /  zebrafish embryos and siblings at 24 hpf", "E MTAB 9113:sibling 1 p", "sibling 1 p", "RNA seq of sfpq /  zebrafish embryos and siblings at 24 hpf", "Embryos were collected from in crosses of sfpq+/  adult zebrafish RNA was extracted using the RNEasy Mini Kit Qiagen Library constructed with ribodepletion", "Experimental Factor: genotype:wild type genotype", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "ERP121885", "Illumina HiSeq 2500 paired end sequencing; RNA seq of sfpq /  zebrafish embryos and siblings at 24 hpf", "ENA FIRST PUBLIC:2021 01 27|ENA LAST UPDATE:2020 06 19", "UCLGNS1141-gfp-sibs-1_S2_R1.fastq.gz UCLGNS1141-gfp-sibs-1_S2_R2.fastq.gz", "fastq fastq", null, null, "E MTAB 9113:UCLGNS1141 gfp sibs 1 S2 R", "0:81 1:81", "A:1121176859;C:1093927654;G:1108608188;T:1101082277;N:209264", 81, 81, null, null, 1121176859, 1093927654, 1108608188, 1101082277, 209264, "ERX4136407", "ERS4580817", "ERA2625401", "Centre for Developmental Neurobiology King", "Centre for Developmental Neurobiology King", 2, 0.95795, 0.96004, 0.02885, 0.02854, 0.71648, 0.71756, 0.44348, 0.43956, 81, 81, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "United Kingdom", "2020-05-22", "Multi-stage", "Multi-stage", "Embryo Imprecise", "All anatomical structures"], [9895, "ERR4172792", "ERX4136406", "ERS4580816", "ERP121885", "PRJEB38455", "RNA seq of sfpq /  zebrafish embryos and siblings at 24 hpf", "E-MTAB-9113", "Transcriptome Analysis", "RNA was extracted from whole zebrafish embryos at 24 hpf to examine changes in gene expression and splicing in sfpq null mutants", "ENA FIRST PUBLIC:2021 01 27|ENA LAST UPDATE:2020 05 22", null, "Protocols: Embryos were collected from in crosses of sfpq+/  adult zebrafish RNA was extracted using the RNEasy Mini Kit Qiagen Library constructed with ribodepletion", "sfpq 3", "SAMEA6853226", "Centre for Developmental Neurobiology King's College London", "ENA FIRST PUBLIC:2020 12 01T04:08:08Z|ENA LAST UPDATE:2020 05 22T17:13:15Z|External Id:SAMEA6853226|INSDC center name:Centre for Developmental Neurobiology King's College London|INSDC first public:2020 12 01T04:08:08Z|INSDC last update:2020 05 22T17:13:15Z|INSDC status:public|Submitter Id:E MTAB 9113:sfpq 3|age:24|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo|genotype:sfpq / |sample name:E MTAB 9113:sfpq 3|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Illumina HiSeq 2500 paired end sequencing; RNA seq of sfpq /  zebrafish embryos and siblings at 24 hpf", "E MTAB 9113:sfpq 3 p", "sfpq 3 p", "RNA seq of sfpq /  zebrafish embryos and siblings at 24 hpf", "Embryos were collected from in crosses of sfpq+/  adult zebrafish RNA was extracted using the RNEasy Mini Kit Qiagen Library constructed with ribodepletion", "Experimental Factor: genotype:sfpq / ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "ERP121885", "Illumina HiSeq 2500 paired end sequencing; RNA seq of sfpq /  zebrafish embryos and siblings at 24 hpf", "ENA FIRST PUBLIC:2021 01 27|ENA LAST UPDATE:2020 06 19", "UCLGNS1141-gfp-neg_3_S5_R1.fastq.gz UCLGNS1141-gfp-neg_3_S5_R2.fastq.gz", "fastq fastq", null, null, "E MTAB 9113:UCLGNS1141 gfp neg 3 S5 R", "0:81 1:81", "A:845849249;C:772302798;G:911660213;T:787409272;N:154042", 81, 81, null, null, 845849249, 772302798, 911660213, 787409272, 154042, "ERX4136406", "ERS4580816", "ERA2625401", "Centre for Developmental Neurobiology King", "Centre for Developmental Neurobiology King", 2, 0.96298, 0.95486, 0.03415, 0.03476, 0.7167, 0.73503, 0.4665, 0.45947, 81, 81, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "United Kingdom", "2020-05-22", "Multi-stage", "Multi-stage", "Embryo Imprecise", "All anatomical structures"], [9896, "ERR4172791", "ERX4136405", "ERS4580815", "ERP121885", "PRJEB38455", "RNA seq of sfpq /  zebrafish embryos and siblings at 24 hpf", "E-MTAB-9113", "Transcriptome Analysis", "RNA was extracted from whole zebrafish embryos at 24 hpf to examine changes in gene expression and splicing in sfpq null mutants", "ENA FIRST PUBLIC:2021 01 27|ENA LAST UPDATE:2020 05 22", null, "Protocols: Embryos were collected from in crosses of sfpq+/  adult zebrafish RNA was extracted using the RNEasy Mini Kit Qiagen Library constructed with ribodepletion", "sfpq 2", "SAMEA6853225", "Centre for Developmental Neurobiology King's College London", "ENA FIRST PUBLIC:2020 12 01T04:08:08Z|ENA LAST UPDATE:2020 05 22T17:13:15Z|External Id:SAMEA6853225|INSDC center name:Centre for Developmental Neurobiology King's College London|INSDC first public:2020 12 01T04:08:08Z|INSDC last update:2020 05 22T17:13:15Z|INSDC status:public|Submitter Id:E MTAB 9113:sfpq 2|age:24|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo|genotype:sfpq / |sample name:E MTAB 9113:sfpq 2|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Illumina HiSeq 2500 paired end sequencing; RNA seq of sfpq /  zebrafish embryos and siblings at 24 hpf", "E MTAB 9113:sfpq 2 p", "sfpq 2 p", "RNA seq of sfpq /  zebrafish embryos and siblings at 24 hpf", "Embryos were collected from in crosses of sfpq+/  adult zebrafish RNA was extracted using the RNEasy Mini Kit Qiagen Library constructed with ribodepletion", "Experimental Factor: genotype:sfpq / ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "ERP121885", "Illumina HiSeq 2500 paired end sequencing; RNA seq of sfpq /  zebrafish embryos and siblings at 24 hpf", "ENA FIRST PUBLIC:2021 01 27|ENA LAST UPDATE:2020 06 19", "UCLGNS1141-gfp-neg-2_S3_R1.fastq.gz UCLGNS1141-gfp-neg-2_S3_R2.fastq.gz", "fastq fastq", null, null, "E MTAB 9113:UCLGNS1141 gfp neg 2 S3 R", "0:81 1:81", "A:961156886;C:917011292;G:942713581;T:936109877;N:157724", 81, 81, null, null, 961156886, 917011292, 942713581, 936109877, 157724, "ERX4136405", "ERS4580815", "ERA2625401", "Centre for Developmental Neurobiology King", "Centre for Developmental Neurobiology King", 2, 0.96276, 0.96147, 0.03436, 0.03382, 0.71892, 0.72021, 0.4581, 0.46402, 81, 81, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "United Kingdom", "2020-05-22", "Multi-stage", "Multi-stage", "Embryo Imprecise", "All anatomical structures"], [9897, "ERR4172790", "ERX4136404", "ERS4580814", "ERP121885", "PRJEB38455", "RNA seq of sfpq /  zebrafish embryos and siblings at 24 hpf", "E-MTAB-9113", "Transcriptome Analysis", "RNA was extracted from whole zebrafish embryos at 24 hpf to examine changes in gene expression and splicing in sfpq null mutants", "ENA FIRST PUBLIC:2021 01 27|ENA LAST UPDATE:2020 05 22", null, "Protocols: Embryos were collected from in crosses of sfpq+/  adult zebrafish RNA was extracted using the RNEasy Mini Kit Qiagen Library constructed with ribodepletion", "sfpq 1", "SAMEA6853224", "Centre for Developmental Neurobiology King's College London", "ENA FIRST PUBLIC:2020 12 01T04:08:08Z|ENA LAST UPDATE:2020 05 22T17:13:15Z|External Id:SAMEA6853224|INSDC center name:Centre for Developmental Neurobiology King's College London|INSDC first public:2020 12 01T04:08:08Z|INSDC last update:2020 05 22T17:13:15Z|INSDC status:public|Submitter Id:E MTAB 9113:sfpq 1|age:24|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo|genotype:sfpq / |sample name:E MTAB 9113:sfpq 1|scientific name:Danio rerio", null, null, null, null, null, null, null, null, "Illumina HiSeq 2500 paired end sequencing; RNA seq of sfpq /  zebrafish embryos and siblings at 24 hpf", "E MTAB 9113:sfpq 1 p", "sfpq 1 p", "RNA seq of sfpq /  zebrafish embryos and siblings at 24 hpf", "Embryos were collected from in crosses of sfpq+/  adult zebrafish RNA was extracted using the RNEasy Mini Kit Qiagen Library constructed with ribodepletion", "Experimental Factor: genotype:sfpq / ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "ERP121885", "Illumina HiSeq 2500 paired end sequencing; RNA seq of sfpq /  zebrafish embryos and siblings at 24 hpf", "ENA FIRST PUBLIC:2021 01 27|ENA LAST UPDATE:2020 06 19", "UCLGNS1141-gfp-neg-1_S1_R1.fastq.gz UCLGNS1141-gfp-neg-1_S1_R2.fastq.gz", "fastq fastq", null, null, "E MTAB 9113:UCLGNS1141 gfp neg 1 S1 R", "0:81 1:81", "A:1046317524;C:1014946275;G:1036910299;T:1023619857;N:185237", 81, 81, null, null, 1046317524, 1014946275, 1036910299, 1023619857, 185237, "ERX4136404", "ERS4580814", "ERA2625401", "Centre for Developmental Neurobiology King", "Centre for Developmental Neurobiology King", 2, 0.96015, 0.96173, 0.03114, 0.03098, 0.7175, 0.71865, 0.46666, 0.46399, 81, 81, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "United Kingdom", "2020-05-22", "Multi-stage", "Multi-stage", "Embryo Imprecise", "All anatomical structures"], [10285, "ERR7132868", "ERX6700306", "ERS8071630", "ERP132573", "PRJEB48231", "RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "E-MTAB-11086", "Transcriptome Analysis", "RNA seq of wild type T\u00fcpfel long fin TLF zebrafish embryos developmentally exposed to three addictive drugs   5 \u00b5M nicotine  1.14 \u00b5M oxycodone and 5 \u00b5M amphetamine. Each of the 24 samples 6 samples per drug  plus 6 control samples represents RNA from a pool of seven 5 dpf zebrafish embryos  exposed to the drug between 1 dpf and 5 dpf.", "ENA FIRST PUBLIC:2022 01 24|ENA LAST UPDATE:2022 01 24", null, "Protocols: Zebrafish embryos were exposed to a drug from xxx dpf ttwo xxx dpf and at the end of the exposure period  larvae were collected as 6 pools of 7 embryos per condition for RNA extraction. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was removed without xxx the beads. Whilst still on the magnet  beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalogue number M0303L. Illumina TruSeq Stranded mRNA Sample Prep Kit.", "Oxy 6", "SAMEA10418786", "Cambridge Institute of Therapeutic Immunology & Infectious Disease", "ENA first public:2022 01 24|ENA last update:2022 01 24|External Id:SAMEA10418786|INSDC center alias:Cambridge Institute of Therapeutic Immunology & Infectious Disease|INSDC center name:Cambridge Institute of Therapeutic Immunology & Infectious Disease|INSDC first public:2022 01 24T16:27:32Z|INSDC last update:2022 01 24T16:27:32Z|INSDC status:public|Submitter Id:E MTAB 11086:Oxy 6|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval day 5|genotype:wild type genotype|individual:pool of 7 embryos|organism part:whole organism|replicate:6|sample name:E MTAB 11086:Oxy 6|sex:not available|strain:T\u00fcpfel long fin TLF", null, null, null, null, null, null, null, null, "Illumina NovaSeq 6000 paired end sequencing; RNA seq of wild type zebrafish embryos developmentally exposed to 3 addictive drugs", "E MTAB 11086:Oxy 6 p", "Oxy 6 p", "RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "Zebrafish embryos were exposed to a drug from xxx dpf ttwo xxx dpf and at the end of the exposure period  larvae were collected as 6 pools of 7 embryos per condition for RNA extraction. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was removed without xxx the beads. Whilst still on the magnet  beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalogue number M0303L. Illumina TruSeq Stranded mRNA Sample Prep Kit.", "Experimental Factor: compound:oxycod1|Experimental Factor: dose:1.14", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP132573", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "ENA FIRST PUBLIC:2022 01 24|ENA LAST UPDATE:2022 01 24", "SLX-19351.B11.HMLG7DRXX.s_2.r_1.fq.gz SLX-19351.B11.HMLG7DRXX.s_2.r_2.fq.gz", "fastq fastq", 2330698400.0, 23306984.0, "E MTAB 11086:SLX 19351.B11.HMLG7DRXX.s 2.r ", "0:50 1:50", "A:619569172;C:544582535;G:551543197;T:614938437;N:65059", 50, 50, null, null, 619569172, 544582535, 551543197, 614938437, 65059, "ERX6700306", "ERS8071630", "ERA6757821", "Cambridge Institute of Therapeutic Immunology & Infectious Disease|European Nucleotide Archive", "Cambridge Institute of Therapeutic Immunology & Infectious Disease|European Nucleotide Archive", 2, 0.9448, 0.95044, 0.1029, 0.09965, 0.67152, 0.66864, 0.46844, 0.47553, 50, 50, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "United Kingdom", "2022-01-24", "Larval", "Larval", "Embryo Imprecise", "All anatomical structures"], [10286, "ERR7132867", "ERX6700305", "ERS8071629", "ERP132573", "PRJEB48231", "RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "E-MTAB-11086", "Transcriptome Analysis", "RNA seq of wild type T\u00fcpfel long fin TLF zebrafish embryos developmentally exposed to three addictive drugs   5 \u00b5M nicotine  1.14 \u00b5M oxycodone and 5 \u00b5M amphetamine. Each of the 24 samples 6 samples per drug  plus 6 control samples represents RNA from a pool of seven 5 dpf zebrafish embryos  exposed to the drug between 1 dpf and 5 dpf.", "ENA FIRST PUBLIC:2022 01 24|ENA LAST UPDATE:2022 01 24", null, "Protocols: Zebrafish embryos were exposed to a drug from xxx dpf ttwo xxx dpf and at the end of the exposure period  larvae were collected as 6 pools of 7 embryos per condition for RNA extraction. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was removed without xxx the beads. Whilst still on the magnet  beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalogue number M0303L. Illumina TruSeq Stranded mRNA Sample Prep Kit.", "Oxy 5", "SAMEA10418785", "Cambridge Institute of Therapeutic Immunology & Infectious Disease", "ENA first public:2022 01 24|ENA last update:2022 01 24|External Id:SAMEA10418785|INSDC center alias:Cambridge Institute of Therapeutic Immunology & Infectious Disease|INSDC center name:Cambridge Institute of Therapeutic Immunology & Infectious Disease|INSDC first public:2022 01 24T16:27:32Z|INSDC last update:2022 01 24T16:27:32Z|INSDC status:public|Submitter Id:E MTAB 11086:Oxy 5|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval day 5|genotype:wild type genotype|individual:pool of 7 embryos|organism part:whole organism|replicate:5|sample name:E MTAB 11086:Oxy 5|sex:not available|strain:T\u00fcpfel long fin TLF", null, null, null, null, null, null, null, null, "Illumina NovaSeq 6000 paired end sequencing; RNA seq of wild type zebrafish embryos developmentally exposed to 3 addictive drugs", "E MTAB 11086:Oxy 5 p", "Oxy 5 p", "RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "Zebrafish embryos were exposed to a drug from xxx dpf ttwo xxx dpf and at the end of the exposure period  larvae were collected as 6 pools of 7 embryos per condition for RNA extraction. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was removed without xxx the beads. Whilst still on the magnet  beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalogue number M0303L. Illumina TruSeq Stranded mRNA Sample Prep Kit.", "Experimental Factor: compound:oxycod1|Experimental Factor: dose:1.14", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP132573", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "ENA FIRST PUBLIC:2022 01 24|ENA LAST UPDATE:2022 01 24", "SLX-19351.H9.HMLG7DRXX.s_2.r_1.fq.gz SLX-19351.H9.HMLG7DRXX.s_2.r_2.fq.gz", "fastq fastq", 2005314000.0, 20053140.0, "E MTAB 11086:SLX 19351.H9.HMLG7DRXX.s 2.r ", "0:50 1:50", "A:533671597;C:466449227;G:473126638;T:532010412;N:56126", 50, 50, null, null, 533671597, 466449227, 473126638, 532010412, 56126, "ERX6700305", "ERS8071629", "ERA6757821", "Cambridge Institute of Therapeutic Immunology & Infectious Disease|European Nucleotide Archive", "Cambridge Institute of Therapeutic Immunology & Infectious Disease|European Nucleotide Archive", 2, 0.94406, 0.94793, 0.11192, 0.10798, 0.66184, 0.66074, 0.4603, 0.4683, 50, 50, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "United Kingdom", "2022-01-24", "Larval", "Larval", "Embryo Imprecise", "All anatomical structures"], [10287, "ERR7132866", "ERX6700304", "ERS8071628", "ERP132573", "PRJEB48231", "RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "E-MTAB-11086", "Transcriptome Analysis", "RNA seq of wild type T\u00fcpfel long fin TLF zebrafish embryos developmentally exposed to three addictive drugs   5 \u00b5M nicotine  1.14 \u00b5M oxycodone and 5 \u00b5M amphetamine. Each of the 24 samples 6 samples per drug  plus 6 control samples represents RNA from a pool of seven 5 dpf zebrafish embryos  exposed to the drug between 1 dpf and 5 dpf.", "ENA FIRST PUBLIC:2022 01 24|ENA LAST UPDATE:2022 01 24", null, "Protocols: Zebrafish embryos were exposed to a drug from xxx dpf ttwo xxx dpf and at the end of the exposure period  larvae were collected as 6 pools of 7 embryos per condition for RNA extraction. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was removed without xxx the beads. Whilst still on the magnet  beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalogue number M0303L. Illumina TruSeq Stranded mRNA Sample Prep Kit.", "Oxy 4", "SAMEA10418784", "Cambridge Institute of Therapeutic Immunology & Infectious Disease", "ENA first public:2022 01 24|ENA last update:2022 01 24|External Id:SAMEA10418784|INSDC center alias:Cambridge Institute of Therapeutic Immunology & Infectious Disease|INSDC center name:Cambridge Institute of Therapeutic Immunology & Infectious Disease|INSDC first public:2022 01 24T16:27:32Z|INSDC last update:2022 01 24T16:27:32Z|INSDC status:public|Submitter Id:E MTAB 11086:Oxy 4|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval day 5|genotype:wild type genotype|individual:pool of 7 embryos|organism part:whole organism|replicate:4|sample name:E MTAB 11086:Oxy 4|sex:not available|strain:T\u00fcpfel long fin TLF", null, null, null, null, null, null, null, null, "Illumina NovaSeq 6000 paired end sequencing; RNA seq of wild type zebrafish embryos developmentally exposed to 3 addictive drugs", "E MTAB 11086:Oxy 4 p", "Oxy 4 p", "RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "Zebrafish embryos were exposed to a drug from xxx dpf ttwo xxx dpf and at the end of the exposure period  larvae were collected as 6 pools of 7 embryos per condition for RNA extraction. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was removed without xxx the beads. Whilst still on the magnet  beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalogue number M0303L. Illumina TruSeq Stranded mRNA Sample Prep Kit.", "Experimental Factor: compound:oxycod1|Experimental Factor: dose:1.14", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP132573", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "ENA FIRST PUBLIC:2022 01 24|ENA LAST UPDATE:2022 01 24", "SLX-19351.A9.HMLG7DRXX.s_2.r_1.fq.gz SLX-19351.A9.HMLG7DRXX.s_2.r_2.fq.gz", "fastq fastq", 2036392600.0, 20363926.0, "E MTAB 11086:SLX 19351.A9.HMLG7DRXX.s 2.r ", "0:50 1:50", "A:547586305;C:468797712;G:476477515;T:543474510;N:56558", 50, 50, null, null, 547586305, 468797712, 476477515, 543474510, 56558, "ERX6700304", "ERS8071628", "ERA6757821", "Cambridge Institute of Therapeutic Immunology & Infectious Disease|European Nucleotide Archive", "Cambridge Institute of Therapeutic Immunology & Infectious Disease|European Nucleotide Archive", 2, 0.94235, 0.94873, 0.11388, 0.11054, 0.67655, 0.67294, 0.47135, 0.47234, 50, 50, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "United Kingdom", "2022-01-24", "Larval", "Larval", "Embryo Imprecise", "All anatomical structures"], [10288, "ERR7132865", "ERX6700303", "ERS8071627", "ERP132573", "PRJEB48231", "RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "E-MTAB-11086", "Transcriptome Analysis", "RNA seq of wild type T\u00fcpfel long fin TLF zebrafish embryos developmentally exposed to three addictive drugs   5 \u00b5M nicotine  1.14 \u00b5M oxycodone and 5 \u00b5M amphetamine. Each of the 24 samples 6 samples per drug  plus 6 control samples represents RNA from a pool of seven 5 dpf zebrafish embryos  exposed to the drug between 1 dpf and 5 dpf.", "ENA FIRST PUBLIC:2022 01 24|ENA LAST UPDATE:2022 01 24", null, "Protocols: Zebrafish embryos were exposed to a drug from xxx dpf ttwo xxx dpf and at the end of the exposure period  larvae were collected as 6 pools of 7 embryos per condition for RNA extraction. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was removed without xxx the beads. Whilst still on the magnet  beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalogue number M0303L. Illumina TruSeq Stranded mRNA Sample Prep Kit.", "Oxy 3", "SAMEA10418783", "Cambridge Institute of Therapeutic Immunology & Infectious Disease", "ENA first public:2022 01 24|ENA last update:2022 01 24|External Id:SAMEA10418783|INSDC center alias:Cambridge Institute of Therapeutic Immunology & Infectious Disease|INSDC center name:Cambridge Institute of Therapeutic Immunology & Infectious Disease|INSDC first public:2022 01 24T16:27:32Z|INSDC last update:2022 01 24T16:27:32Z|INSDC status:public|Submitter Id:E MTAB 11086:Oxy 3|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval day 5|genotype:wild type genotype|individual:pool of 7 embryos|organism part:whole organism|replicate:3|sample name:E MTAB 11086:Oxy 3|sex:not available|strain:T\u00fcpfel long fin TLF", null, null, null, null, null, null, null, null, "Illumina NovaSeq 6000 paired end sequencing; RNA seq of wild type zebrafish embryos developmentally exposed to 3 addictive drugs", "E MTAB 11086:Oxy 3 p", "Oxy 3 p", "RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "Zebrafish embryos were exposed to a drug from xxx dpf ttwo xxx dpf and at the end of the exposure period  larvae were collected as 6 pools of 7 embryos per condition for RNA extraction. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was removed without xxx the beads. Whilst still on the magnet  beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalogue number M0303L. Illumina TruSeq Stranded mRNA Sample Prep Kit.", "Experimental Factor: compound:oxycod1|Experimental Factor: dose:1.14", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP132573", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "ENA FIRST PUBLIC:2022 01 24|ENA LAST UPDATE:2022 01 24", "SLX-19351.B9.HMLG7DRXX.s_2.r_1.fq.gz SLX-19351.B9.HMLG7DRXX.s_2.r_2.fq.gz", "fastq fastq", 2409819000.0, 24098190.0, "E MTAB 11086:SLX 19351.B9.HMLG7DRXX.s 2.r ", "0:50 1:50", "A:641053989;C:561685242;G:569693271;T:637320302;N:66196", 50, 50, null, null, 641053989, 561685242, 569693271, 637320302, 66196, "ERX6700303", "ERS8071627", "ERA6757821", "Cambridge Institute of Therapeutic Immunology & Infectious Disease|European Nucleotide Archive", "Cambridge Institute of Therapeutic Immunology & Infectious Disease|European Nucleotide Archive", 2, 0.94407, 0.94902, 0.10789, 0.10496, 0.66478, 0.66387, 0.47154, 0.47373, 50, 50, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "United Kingdom", "2022-01-24", "Larval", "Larval", "Embryo Imprecise", "All anatomical structures"], [10289, "ERR7132864", "ERX6700302", "ERS8071626", "ERP132573", "PRJEB48231", "RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "E-MTAB-11086", "Transcriptome Analysis", "RNA seq of wild type T\u00fcpfel long fin TLF zebrafish embryos developmentally exposed to three addictive drugs   5 \u00b5M nicotine  1.14 \u00b5M oxycodone and 5 \u00b5M amphetamine. Each of the 24 samples 6 samples per drug  plus 6 control samples represents RNA from a pool of seven 5 dpf zebrafish embryos  exposed to the drug between 1 dpf and 5 dpf.", "ENA FIRST PUBLIC:2022 01 24|ENA LAST UPDATE:2022 01 24", null, "Protocols: Zebrafish embryos were exposed to a drug from xxx dpf ttwo xxx dpf and at the end of the exposure period  larvae were collected as 6 pools of 7 embryos per condition for RNA extraction. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was removed without xxx the beads. Whilst still on the magnet  beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalogue number M0303L. Illumina TruSeq Stranded mRNA Sample Prep Kit.", "Oxy 2", "SAMEA10418782", "Cambridge Institute of Therapeutic Immunology & Infectious Disease", "ENA first public:2022 01 24|ENA last update:2022 01 24|External Id:SAMEA10418782|INSDC center alias:Cambridge Institute of Therapeutic Immunology & Infectious Disease|INSDC center name:Cambridge Institute of Therapeutic Immunology & Infectious Disease|INSDC first public:2022 01 24T16:27:32Z|INSDC last update:2022 01 24T16:27:32Z|INSDC status:public|Submitter Id:E MTAB 11086:Oxy 2|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval day 5|genotype:wild type genotype|individual:pool of 7 embryos|organism part:whole organism|replicate:2|sample name:E MTAB 11086:Oxy 2|sex:not available|strain:T\u00fcpfel long fin TLF", null, null, null, null, null, null, null, null, "Illumina NovaSeq 6000 paired end sequencing; RNA seq of wild type zebrafish embryos developmentally exposed to 3 addictive drugs", "E MTAB 11086:Oxy 2 p", "Oxy 2 p", "RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "Zebrafish embryos were exposed to a drug from xxx dpf ttwo xxx dpf and at the end of the exposure period  larvae were collected as 6 pools of 7 embryos per condition for RNA extraction. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was removed without xxx the beads. Whilst still on the magnet  beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalogue number M0303L. Illumina TruSeq Stranded mRNA Sample Prep Kit.", "Experimental Factor: compound:oxycod1|Experimental Factor: dose:1.14", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP132573", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "ENA FIRST PUBLIC:2022 01 24|ENA LAST UPDATE:2022 01 24", "SLX-19351.A11.HMLG7DRXX.s_2.r_1.fq.gz SLX-19351.A11.HMLG7DRXX.s_2.r_2.fq.gz", "fastq fastq", 2068575200.0, 20685752.0, "E MTAB 11086:SLX 19351.A11.HMLG7DRXX.s 2.r ", "0:50 1:50", "A:547986796;C:484533693;G:490663629;T:545334125;N:56957", 50, 50, null, null, 547986796, 484533693, 490663629, 545334125, 56957, "ERX6700302", "ERS8071626", "ERA6757821", "Cambridge Institute of Therapeutic Immunology & Infectious Disease|European Nucleotide Archive", "Cambridge Institute of Therapeutic Immunology & Infectious Disease|European Nucleotide Archive", 2, 0.94539, 0.95188, 0.10373, 0.10116, 0.66718, 0.66584, 0.46991, 0.47611, 50, 50, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "United Kingdom", "2022-01-24", "Larval", "Larval", "Embryo Imprecise", "All anatomical structures"], [10290, "ERR7132863", "ERX6700301", "ERS8071625", "ERP132573", "PRJEB48231", "RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "E-MTAB-11086", "Transcriptome Analysis", "RNA seq of wild type T\u00fcpfel long fin TLF zebrafish embryos developmentally exposed to three addictive drugs   5 \u00b5M nicotine  1.14 \u00b5M oxycodone and 5 \u00b5M amphetamine. Each of the 24 samples 6 samples per drug  plus 6 control samples represents RNA from a pool of seven 5 dpf zebrafish embryos  exposed to the drug between 1 dpf and 5 dpf.", "ENA FIRST PUBLIC:2022 01 24|ENA LAST UPDATE:2022 01 24", null, "Protocols: Zebrafish embryos were exposed to a drug from xxx dpf ttwo xxx dpf and at the end of the exposure period  larvae were collected as 6 pools of 7 embryos per condition for RNA extraction. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was removed without xxx the beads. Whilst still on the magnet  beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalogue number M0303L. Illumina TruSeq Stranded mRNA Sample Prep Kit.", "Oxy 1", "SAMEA10418781", "Cambridge Institute of Therapeutic Immunology & Infectious Disease", "ENA first public:2022 01 24|ENA last update:2022 01 24|External Id:SAMEA10418781|INSDC center alias:Cambridge Institute of Therapeutic Immunology & Infectious Disease|INSDC center name:Cambridge Institute of Therapeutic Immunology & Infectious Disease|INSDC first public:2022 01 24T16:27:32Z|INSDC last update:2022 01 24T16:27:32Z|INSDC status:public|Submitter Id:E MTAB 11086:Oxy 1|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval day 5|genotype:wild type genotype|individual:pool of 7 embryos|organism part:whole organism|replicate:1|sample name:E MTAB 11086:Oxy 1|sex:not available|strain:T\u00fcpfel long fin TLF", null, null, null, null, null, null, null, null, "Illumina NovaSeq 6000 paired end sequencing; RNA seq of wild type zebrafish embryos developmentally exposed to 3 addictive drugs", "E MTAB 11086:Oxy 1 p", "Oxy 1 p", "RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "Zebrafish embryos were exposed to a drug from xxx dpf ttwo xxx dpf and at the end of the exposure period  larvae were collected as 6 pools of 7 embryos per condition for RNA extraction. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was removed without xxx the beads. Whilst still on the magnet  beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalogue number M0303L. Illumina TruSeq Stranded mRNA Sample Prep Kit.", "Experimental Factor: compound:oxycod1|Experimental Factor: dose:1.14", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP132573", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "ENA FIRST PUBLIC:2022 01 24|ENA LAST UPDATE:2022 01 24", "SLX-19351.G9.HMLG7DRXX.s_2.r_1.fq.gz SLX-19351.G9.HMLG7DRXX.s_2.r_2.fq.gz", "fastq fastq", 2101607900.0, 21016079.0, "E MTAB 11086:SLX 19351.G9.HMLG7DRXX.s 2.r ", "0:50 1:50", "A:557420271;C:491161431;G:497494262;T:555471610;N:60326", 50, 50, null, null, 557420271, 491161431, 497494262, 555471610, 60326, "ERX6700301", "ERS8071625", "ERA6757821", "Cambridge Institute of Therapeutic Immunology & Infectious Disease|European Nucleotide Archive", "Cambridge Institute of Therapeutic Immunology & Infectious Disease|European Nucleotide Archive", 2, 0.94378, 0.94916, 0.10779, 0.10431, 0.66507, 0.66377, 0.45477, 0.4679, 50, 50, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "United Kingdom", "2022-01-24", "Larval", "Larval", "Embryo Imprecise", "All anatomical structures"], [10291, "ERR7132862", "ERX6700300", "ERS8071624", "ERP132573", "PRJEB48231", "RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "E-MTAB-11086", "Transcriptome Analysis", "RNA seq of wild type T\u00fcpfel long fin TLF zebrafish embryos developmentally exposed to three addictive drugs   5 \u00b5M nicotine  1.14 \u00b5M oxycodone and 5 \u00b5M amphetamine. Each of the 24 samples 6 samples per drug  plus 6 control samples represents RNA from a pool of seven 5 dpf zebrafish embryos  exposed to the drug between 1 dpf and 5 dpf.", "ENA FIRST PUBLIC:2022 01 24|ENA LAST UPDATE:2022 01 24", null, "Protocols: Zebrafish embryos were exposed to a drug from xxx dpf ttwo xxx dpf and at the end of the exposure period  larvae were collected as 6 pools of 7 embryos per condition for RNA extraction. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was removed without xxx the beads. Whilst still on the magnet  beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalogue number M0303L. Illumina TruSeq Stranded mRNA Sample Prep Kit.", "Nic 6", "SAMEA10418780", "Cambridge Institute of Therapeutic Immunology & Infectious Disease", "ENA first public:2022 01 24|ENA last update:2022 01 24|External Id:SAMEA10418780|INSDC center alias:Cambridge Institute of Therapeutic Immunology & Infectious Disease|INSDC center name:Cambridge Institute of Therapeutic Immunology & Infectious Disease|INSDC first public:2022 01 24T16:27:32Z|INSDC last update:2022 01 24T16:27:32Z|INSDC status:public|Submitter Id:E MTAB 11086:Nic 6|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval day 5|genotype:wild type genotype|individual:pool of 7 embryos|organism part:whole organism|replicate:6|sample name:E MTAB 11086:Nic 6|sex:not available|strain:T\u00fcpfel long fin TLF", null, null, null, null, null, null, null, null, "Illumina NovaSeq 6000 paired end sequencing; RNA seq of wild type zebrafish embryos developmentally exposed to 3 addictive drugs", "E MTAB 11086:Nic 6 p", "Nic 6 p", "RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "Zebrafish embryos were exposed to a drug from xxx dpf ttwo xxx dpf and at the end of the exposure period  larvae were collected as 6 pools of 7 embryos per condition for RNA extraction. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was removed without xxx the beads. Whilst still on the magnet  beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalogue number M0303L. Illumina TruSeq Stranded mRNA Sample Prep Kit.", "Experimental Factor: compound:nicotine|Experimental Factor: dose:5", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP132573", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "ENA FIRST PUBLIC:2022 01 24|ENA LAST UPDATE:2022 01 24", "SLX-19351.H11.HMLG7DRXX.s_2.r_1.fq.gz SLX-19351.H11.HMLG7DRXX.s_2.r_2.fq.gz", "fastq fastq", 2236235700.0, 22362357.0, "E MTAB 11086:SLX 19351.H11.HMLG7DRXX.s 2.r ", "0:50 1:50", "A:597573047;C:518264376;G:525314754;T:595021334;N:62189", 50, 50, null, null, 597573047, 518264376, 525314754, 595021334, 62189, "ERX6700300", "ERS8071624", "ERA6757821", "Cambridge Institute of Therapeutic Immunology & Infectious Disease|European Nucleotide Archive", "Cambridge Institute of Therapeutic Immunology & Infectious Disease|European Nucleotide Archive", 2, 0.94255, 0.94893, 0.11268, 0.10955, 0.66819, 0.66687, 0.46882, 0.47485, 50, 50, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "United Kingdom", "2022-01-24", "Larval", "Larval", "Embryo Imprecise", "All anatomical structures"], [10292, "ERR7132861", "ERX6700299", "ERS8071623", "ERP132573", "PRJEB48231", "RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "E-MTAB-11086", "Transcriptome Analysis", "RNA seq of wild type T\u00fcpfel long fin TLF zebrafish embryos developmentally exposed to three addictive drugs   5 \u00b5M nicotine  1.14 \u00b5M oxycodone and 5 \u00b5M amphetamine. Each of the 24 samples 6 samples per drug  plus 6 control samples represents RNA from a pool of seven 5 dpf zebrafish embryos  exposed to the drug between 1 dpf and 5 dpf.", "ENA FIRST PUBLIC:2022 01 24|ENA LAST UPDATE:2022 01 24", null, "Protocols: Zebrafish embryos were exposed to a drug from xxx dpf ttwo xxx dpf and at the end of the exposure period  larvae were collected as 6 pools of 7 embryos per condition for RNA extraction. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was removed without xxx the beads. Whilst still on the magnet  beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalogue number M0303L. Illumina TruSeq Stranded mRNA Sample Prep Kit.", "Nic 5", "SAMEA10418779", "Cambridge Institute of Therapeutic Immunology & Infectious Disease", "ENA first public:2022 01 24|ENA last update:2022 01 24|External Id:SAMEA10418779|INSDC center alias:Cambridge Institute of Therapeutic Immunology & Infectious Disease|INSDC center name:Cambridge Institute of Therapeutic Immunology & Infectious Disease|INSDC first public:2022 01 24T16:27:32Z|INSDC last update:2022 01 24T16:27:32Z|INSDC status:public|Submitter Id:E MTAB 11086:Nic 5|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval day 5|genotype:wild type genotype|individual:pool of 7 embryos|organism part:whole organism|replicate:5|sample name:E MTAB 11086:Nic 5|sex:not available|strain:T\u00fcpfel long fin TLF", null, null, null, null, null, null, null, null, "Illumina NovaSeq 6000 paired end sequencing; RNA seq of wild type zebrafish embryos developmentally exposed to 3 addictive drugs", "E MTAB 11086:Nic 5 p", "Nic 5 p", "RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "Zebrafish embryos were exposed to a drug from xxx dpf ttwo xxx dpf and at the end of the exposure period  larvae were collected as 6 pools of 7 embryos per condition for RNA extraction. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was removed without xxx the beads. Whilst still on the magnet  beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalogue number M0303L. Illumina TruSeq Stranded mRNA Sample Prep Kit.", "Experimental Factor: compound:nicotine|Experimental Factor: dose:5", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP132573", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "ENA FIRST PUBLIC:2022 01 24|ENA LAST UPDATE:2022 01 24", "SLX-19351.D10.HMLG7DRXX.s_2.r_1.fq.gz SLX-19351.D10.HMLG7DRXX.s_2.r_2.fq.gz", "fastq fastq", 1904348300.0, 19043483.0, "E MTAB 11086:SLX 19351.D10.HMLG7DRXX.s 2.r ", "0:50 1:50", "A:506678821;C:443756285;G:450118827;T:503742221;N:52146", 50, 50, null, null, 506678821, 443756285, 450118827, 503742221, 52146, "ERX6700299", "ERS8071623", "ERA6757821", "Cambridge Institute of Therapeutic Immunology & Infectious Disease|European Nucleotide Archive", "Cambridge Institute of Therapeutic Immunology & Infectious Disease|European Nucleotide Archive", 2, 0.94468, 0.95019, 0.10313, 0.10023, 0.66762, 0.66513, 0.47749, 0.47611, 50, 50, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "United Kingdom", "2022-01-24", "Larval", "Larval", "Embryo Imprecise", "All anatomical structures"], [10293, "ERR7132860", "ERX6700298", "ERS8071622", "ERP132573", "PRJEB48231", "RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "E-MTAB-11086", "Transcriptome Analysis", "RNA seq of wild type T\u00fcpfel long fin TLF zebrafish embryos developmentally exposed to three addictive drugs   5 \u00b5M nicotine  1.14 \u00b5M oxycodone and 5 \u00b5M amphetamine. Each of the 24 samples 6 samples per drug  plus 6 control samples represents RNA from a pool of seven 5 dpf zebrafish embryos  exposed to the drug between 1 dpf and 5 dpf.", "ENA FIRST PUBLIC:2022 01 24|ENA LAST UPDATE:2022 01 24", null, "Protocols: Zebrafish embryos were exposed to a drug from xxx dpf ttwo xxx dpf and at the end of the exposure period  larvae were collected as 6 pools of 7 embryos per condition for RNA extraction. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was removed without xxx the beads. Whilst still on the magnet  beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalogue number M0303L. Illumina TruSeq Stranded mRNA Sample Prep Kit.", "Nic 4", "SAMEA10418778", "Cambridge Institute of Therapeutic Immunology & Infectious Disease", "ENA first public:2022 01 24|ENA last update:2022 01 24|External Id:SAMEA10418778|INSDC center alias:Cambridge Institute of Therapeutic Immunology & Infectious Disease|INSDC center name:Cambridge Institute of Therapeutic Immunology & Infectious Disease|INSDC first public:2022 01 24T16:27:32Z|INSDC last update:2022 01 24T16:27:32Z|INSDC status:public|Submitter Id:E MTAB 11086:Nic 4|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval day 5|genotype:wild type genotype|individual:pool of 7 embryos|organism part:whole organism|replicate:4|sample name:E MTAB 11086:Nic 4|sex:not available|strain:T\u00fcpfel long fin TLF", null, null, null, null, null, null, null, null, "Illumina NovaSeq 6000 paired end sequencing; RNA seq of wild type zebrafish embryos developmentally exposed to 3 addictive drugs", "E MTAB 11086:Nic 4 p", "Nic 4 p", "RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "Zebrafish embryos were exposed to a drug from xxx dpf ttwo xxx dpf and at the end of the exposure period  larvae were collected as 6 pools of 7 embryos per condition for RNA extraction. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was removed without xxx the beads. Whilst still on the magnet  beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalogue number M0303L. Illumina TruSeq Stranded mRNA Sample Prep Kit.", "Experimental Factor: compound:nicotine|Experimental Factor: dose:5", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP132573", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "ENA FIRST PUBLIC:2022 01 24|ENA LAST UPDATE:2022 01 24", "SLX-19351.C10.HMLG7DRXX.s_2.r_1.fq.gz SLX-19351.C10.HMLG7DRXX.s_2.r_2.fq.gz", "fastq fastq", 2134565600.0, 21345656.0, "E MTAB 11086:SLX 19351.C10.HMLG7DRXX.s 2.r ", "0:50 1:50", "A:568799307;C:496480840;G:503115671;T:566109163;N:60619", 50, 50, null, null, 568799307, 496480840, 503115671, 566109163, 60619, "ERX6700298", "ERS8071622", "ERA6757821", "Cambridge Institute of Therapeutic Immunology & Infectious Disease|European Nucleotide Archive", "Cambridge Institute of Therapeutic Immunology & Infectious Disease|European Nucleotide Archive", 2, 0.94279, 0.94878, 0.11466, 0.1122, 0.66291, 0.66176, 0.46991, 0.47168, 50, 50, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "United Kingdom", "2022-01-24", "Larval", "Larval", "Embryo Imprecise", "All anatomical structures"], [10294, "ERR7132859", "ERX6700297", "ERS8071621", "ERP132573", "PRJEB48231", "RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "E-MTAB-11086", "Transcriptome Analysis", "RNA seq of wild type T\u00fcpfel long fin TLF zebrafish embryos developmentally exposed to three addictive drugs   5 \u00b5M nicotine  1.14 \u00b5M oxycodone and 5 \u00b5M amphetamine. Each of the 24 samples 6 samples per drug  plus 6 control samples represents RNA from a pool of seven 5 dpf zebrafish embryos  exposed to the drug between 1 dpf and 5 dpf.", "ENA FIRST PUBLIC:2022 01 24|ENA LAST UPDATE:2022 01 24", null, "Protocols: Zebrafish embryos were exposed to a drug from xxx dpf ttwo xxx dpf and at the end of the exposure period  larvae were collected as 6 pools of 7 embryos per condition for RNA extraction. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was removed without xxx the beads. Whilst still on the magnet  beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalogue number M0303L. Illumina TruSeq Stranded mRNA Sample Prep Kit.", "Nic 3", "SAMEA10418777", "Cambridge Institute of Therapeutic Immunology & Infectious Disease", "ENA first public:2022 01 24|ENA last update:2022 01 24|External Id:SAMEA10418777|INSDC center alias:Cambridge Institute of Therapeutic Immunology & Infectious Disease|INSDC center name:Cambridge Institute of Therapeutic Immunology & Infectious Disease|INSDC first public:2022 01 24T16:27:32Z|INSDC last update:2022 01 24T16:27:32Z|INSDC status:public|Submitter Id:E MTAB 11086:Nic 3|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval day 5|genotype:wild type genotype|individual:pool of 7 embryos|organism part:whole organism|replicate:3|sample name:E MTAB 11086:Nic 3|sex:not available|strain:T\u00fcpfel long fin TLF", null, null, null, null, null, null, null, null, "Illumina NovaSeq 6000 paired end sequencing; RNA seq of wild type zebrafish embryos developmentally exposed to 3 addictive drugs", "E MTAB 11086:Nic 3 p", "Nic 3 p", "RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "Zebrafish embryos were exposed to a drug from xxx dpf ttwo xxx dpf and at the end of the exposure period  larvae were collected as 6 pools of 7 embryos per condition for RNA extraction. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was removed without xxx the beads. Whilst still on the magnet  beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalogue number M0303L. Illumina TruSeq Stranded mRNA Sample Prep Kit.", "Experimental Factor: compound:nicotine|Experimental Factor: dose:5", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP132573", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "ENA FIRST PUBLIC:2022 01 24|ENA LAST UPDATE:2022 01 24", "SLX-19351.B10.HMLG7DRXX.s_2.r_1.fq.gz SLX-19351.B10.HMLG7DRXX.s_2.r_2.fq.gz", "fastq fastq", 2409355400.0, 24093554.0, "E MTAB 11086:SLX 19351.B10.HMLG7DRXX.s 2.r ", "0:50 1:50", "A:643046449;C:559571909;G:567748411;T:638920943;N:67688", 50, 50, null, null, 643046449, 559571909, 567748411, 638920943, 67688, "ERX6700297", "ERS8071621", "ERA6757821", "Cambridge Institute of Therapeutic Immunology & Infectious Disease|European Nucleotide Archive", "Cambridge Institute of Therapeutic Immunology & Infectious Disease|European Nucleotide Archive", 2, 0.94305, 0.94796, 0.11097, 0.10766, 0.66149, 0.65888, 0.46643, 0.47512, 50, 50, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "United Kingdom", "2022-01-24", "Larval", "Larval", "Embryo Imprecise", "All anatomical structures"], [10295, "ERR7132858", "ERX6700296", "ERS8071620", "ERP132573", "PRJEB48231", "RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "E-MTAB-11086", "Transcriptome Analysis", "RNA seq of wild type T\u00fcpfel long fin TLF zebrafish embryos developmentally exposed to three addictive drugs   5 \u00b5M nicotine  1.14 \u00b5M oxycodone and 5 \u00b5M amphetamine. Each of the 24 samples 6 samples per drug  plus 6 control samples represents RNA from a pool of seven 5 dpf zebrafish embryos  exposed to the drug between 1 dpf and 5 dpf.", "ENA FIRST PUBLIC:2022 01 24|ENA LAST UPDATE:2022 01 24", null, "Protocols: Zebrafish embryos were exposed to a drug from xxx dpf ttwo xxx dpf and at the end of the exposure period  larvae were collected as 6 pools of 7 embryos per condition for RNA extraction. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was removed without xxx the beads. Whilst still on the magnet  beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalogue number M0303L. Illumina TruSeq Stranded mRNA Sample Prep Kit.", "Nic 2", "SAMEA10418776", "Cambridge Institute of Therapeutic Immunology & Infectious Disease", "ENA first public:2022 01 24|ENA last update:2022 01 24|External Id:SAMEA10418776|INSDC center alias:Cambridge Institute of Therapeutic Immunology & Infectious Disease|INSDC center name:Cambridge Institute of Therapeutic Immunology & Infectious Disease|INSDC first public:2022 01 24T16:27:32Z|INSDC last update:2022 01 24T16:27:32Z|INSDC status:public|Submitter Id:E MTAB 11086:Nic 2|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval day 5|genotype:wild type genotype|individual:pool of 7 embryos|organism part:whole organism|replicate:2|sample name:E MTAB 11086:Nic 2|sex:not available|strain:T\u00fcpfel long fin TLF", null, null, null, null, null, null, null, null, "Illumina NovaSeq 6000 paired end sequencing; RNA seq of wild type zebrafish embryos developmentally exposed to 3 addictive drugs", "E MTAB 11086:Nic 2 p", "Nic 2 p", "RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "Zebrafish embryos were exposed to a drug from xxx dpf ttwo xxx dpf and at the end of the exposure period  larvae were collected as 6 pools of 7 embryos per condition for RNA extraction. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was removed without xxx the beads. Whilst still on the magnet  beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalogue number M0303L. Illumina TruSeq Stranded mRNA Sample Prep Kit.", "Experimental Factor: compound:nicotine|Experimental Factor: dose:5", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP132573", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "ENA FIRST PUBLIC:2022 01 24|ENA LAST UPDATE:2022 01 24", "SLX-19351.F10.HMLG7DRXX.s_2.r_1.fq.gz SLX-19351.F10.HMLG7DRXX.s_2.r_2.fq.gz", "fastq fastq", 2046910800.0, 20469108.0, "E MTAB 11086:SLX 19351.F10.HMLG7DRXX.s 2.r ", "0:50 1:50", "A:547096034;C:475610002;G:481041169;T:543104402;N:59193", 50, 50, null, null, 547096034, 475610002, 481041169, 543104402, 59193, "ERX6700296", "ERS8071620", "ERA6757821", "Cambridge Institute of Therapeutic Immunology & Infectious Disease|European Nucleotide Archive", "Cambridge Institute of Therapeutic Immunology & Infectious Disease|European Nucleotide Archive", 2, 0.94391, 0.94965, 0.10824, 0.10557, 0.66241, 0.65963, 0.47747, 0.47375, 50, 50, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "United Kingdom", "2022-01-24", "Larval", "Larval", "Embryo Imprecise", "All anatomical structures"], [10296, "ERR7132857", "ERX6700295", "ERS8071619", "ERP132573", "PRJEB48231", "RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "E-MTAB-11086", "Transcriptome Analysis", "RNA seq of wild type T\u00fcpfel long fin TLF zebrafish embryos developmentally exposed to three addictive drugs   5 \u00b5M nicotine  1.14 \u00b5M oxycodone and 5 \u00b5M amphetamine. Each of the 24 samples 6 samples per drug  plus 6 control samples represents RNA from a pool of seven 5 dpf zebrafish embryos  exposed to the drug between 1 dpf and 5 dpf.", "ENA FIRST PUBLIC:2022 01 24|ENA LAST UPDATE:2022 01 24", null, "Protocols: Zebrafish embryos were exposed to a drug from xxx dpf ttwo xxx dpf and at the end of the exposure period  larvae were collected as 6 pools of 7 embryos per condition for RNA extraction. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was removed without xxx the beads. Whilst still on the magnet  beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalogue number M0303L. Illumina TruSeq Stranded mRNA Sample Prep Kit.", "Nic 1", "SAMEA10418775", "Cambridge Institute of Therapeutic Immunology & Infectious Disease", "ENA first public:2022 01 24|ENA last update:2022 01 24|External Id:SAMEA10418775|INSDC center alias:Cambridge Institute of Therapeutic Immunology & Infectious Disease|INSDC center name:Cambridge Institute of Therapeutic Immunology & Infectious Disease|INSDC first public:2022 01 24T16:27:32Z|INSDC last update:2022 01 24T16:27:32Z|INSDC status:public|Submitter Id:E MTAB 11086:Nic 1|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval day 5|genotype:wild type genotype|individual:pool of 7 embryos|organism part:whole organism|replicate:1|sample name:E MTAB 11086:Nic 1|sex:not available|strain:T\u00fcpfel long fin TLF", null, null, null, null, null, null, null, null, "Illumina NovaSeq 6000 paired end sequencing; RNA seq of wild type zebrafish embryos developmentally exposed to 3 addictive drugs", "E MTAB 11086:Nic 1 p", "Nic 1 p", "RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "Zebrafish embryos were exposed to a drug from xxx dpf ttwo xxx dpf and at the end of the exposure period  larvae were collected as 6 pools of 7 embryos per condition for RNA extraction. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was removed without xxx the beads. Whilst still on the magnet  beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalogue number M0303L. Illumina TruSeq Stranded mRNA Sample Prep Kit.", "Experimental Factor: compound:nicotine|Experimental Factor: dose:5", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP132573", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "ENA FIRST PUBLIC:2022 01 24|ENA LAST UPDATE:2022 01 24", "SLX-19351.G10.HMLG7DRXX.s_2.r_2.fq.gz SLX-19351.G10.HMLG7DRXX.s_2.r_1.fq.gz", "fastq fastq", 2001312600.0, 20013126.0, "E MTAB 11086:SLX 19351.G10.HMLG7DRXX.s 2.r ", "0:50 1:50", "A:535065614;C:463328218;G:468900497;T:533963156;N:55115", 50, 50, null, null, 535065614, 463328218, 468900497, 533963156, 55115, "ERX6700295", "ERS8071619", "ERA6757821", "Cambridge Institute of Therapeutic Immunology & Infectious Disease|European Nucleotide Archive", "Cambridge Institute of Therapeutic Immunology & Infectious Disease|European Nucleotide Archive", 2, 0.94179, 0.9477, 0.11455, 0.11139, 0.66697, 0.66569, 0.45749, 0.47217, 50, 50, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "United Kingdom", "2022-01-24", "Larval", "Larval", "Embryo Imprecise", "All anatomical structures"], [10297, "ERR7132856", "ERX6700294", "ERS8071618", "ERP132573", "PRJEB48231", "RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "E-MTAB-11086", "Transcriptome Analysis", "RNA seq of wild type T\u00fcpfel long fin TLF zebrafish embryos developmentally exposed to three addictive drugs   5 \u00b5M nicotine  1.14 \u00b5M oxycodone and 5 \u00b5M amphetamine. Each of the 24 samples 6 samples per drug  plus 6 control samples represents RNA from a pool of seven 5 dpf zebrafish embryos  exposed to the drug between 1 dpf and 5 dpf.", "ENA FIRST PUBLIC:2022 01 24|ENA LAST UPDATE:2022 01 24", null, "Protocols: Zebrafish embryos were exposed to a drug from xxx dpf ttwo xxx dpf and at the end of the exposure period  larvae were collected as 6 pools of 7 embryos per condition for RNA extraction. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was removed without xxx the beads. Whilst still on the magnet  beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalogue number M0303L. Illumina TruSeq Stranded mRNA Sample Prep Kit.", "Cnt 6", "SAMEA10418774", "Cambridge Institute of Therapeutic Immunology & Infectious Disease", "ENA first public:2022 01 24|ENA last update:2022 01 24|External Id:SAMEA10418774|INSDC center alias:Cambridge Institute of Therapeutic Immunology & Infectious Disease|INSDC center name:Cambridge Institute of Therapeutic Immunology & Infectious Disease|INSDC first public:2022 01 24T16:27:32Z|INSDC last update:2022 01 24T16:27:32Z|INSDC status:public|Submitter Id:E MTAB 11086:Cnt 6|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval day 5|genotype:wild type genotype|individual:pool of 7 embryos|organism part:whole organism|replicate:6|sample name:E MTAB 11086:Cnt 6|sex:not available|strain:T\u00fcpfel long fin TLF", null, null, null, null, null, null, null, null, "Illumina NovaSeq 6000 paired end sequencing; RNA seq of wild type zebrafish embryos developmentally exposed to 3 addictive drugs", "E MTAB 11086:Cnt 6 p", "Cnt 6 p", "RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "Zebrafish embryos were exposed to a drug from xxx dpf ttwo xxx dpf and at the end of the exposure period  larvae were collected as 6 pools of 7 embryos per condition for RNA extraction. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was removed without xxx the beads. Whilst still on the magnet  beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalogue number M0303L. Illumina TruSeq Stranded mRNA Sample Prep Kit.", "Experimental Factor: compound:n1", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP132573", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "ENA FIRST PUBLIC:2022 01 24|ENA LAST UPDATE:2022 01 24", "SLX-19351.C11.HMLG7DRXX.s_2.r_1.fq.gz SLX-19351.C11.HMLG7DRXX.s_2.r_2.fq.gz", "fastq fastq", 2311609400.0, 23116094.0, "E MTAB 11086:SLX 19351.C11.HMLG7DRXX.s 2.r ", "0:50 1:50", "A:616569709;C:538286683;G:545161394;T:611525546;N:66068", 50, 50, null, null, 616569709, 538286683, 545161394, 611525546, 66068, "ERX6700294", "ERS8071618", "ERA6757821", "Cambridge Institute of Therapeutic Immunology & Infectious Disease|European Nucleotide Archive", "Cambridge Institute of Therapeutic Immunology & Infectious Disease|European Nucleotide Archive", 2, 0.94486, 0.95028, 0.1097, 0.10661, 0.66703, 0.6644, 0.47311, 0.47453, 50, 50, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "United Kingdom", "2022-01-24", "Larval", "Larval", "Embryo Imprecise", "All anatomical structures"], [10298, "ERR7132855", "ERX6700293", "ERS8071617", "ERP132573", "PRJEB48231", "RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "E-MTAB-11086", "Transcriptome Analysis", "RNA seq of wild type T\u00fcpfel long fin TLF zebrafish embryos developmentally exposed to three addictive drugs   5 \u00b5M nicotine  1.14 \u00b5M oxycodone and 5 \u00b5M amphetamine. Each of the 24 samples 6 samples per drug  plus 6 control samples represents RNA from a pool of seven 5 dpf zebrafish embryos  exposed to the drug between 1 dpf and 5 dpf.", "ENA FIRST PUBLIC:2022 01 24|ENA LAST UPDATE:2022 01 24", null, "Protocols: Zebrafish embryos were exposed to a drug from xxx dpf ttwo xxx dpf and at the end of the exposure period  larvae were collected as 6 pools of 7 embryos per condition for RNA extraction. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was removed without xxx the beads. Whilst still on the magnet  beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalogue number M0303L. Illumina TruSeq Stranded mRNA Sample Prep Kit.", "Cnt 5", "SAMEA10418773", "Cambridge Institute of Therapeutic Immunology & Infectious Disease", "ENA first public:2022 01 24|ENA last update:2022 01 24|External Id:SAMEA10418773|INSDC center alias:Cambridge Institute of Therapeutic Immunology & Infectious Disease|INSDC center name:Cambridge Institute of Therapeutic Immunology & Infectious Disease|INSDC first public:2022 01 24T16:27:32Z|INSDC last update:2022 01 24T16:27:32Z|INSDC status:public|Submitter Id:E MTAB 11086:Cnt 5|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval day 5|genotype:wild type genotype|individual:pool of 7 embryos|organism part:whole organism|replicate:5|sample name:E MTAB 11086:Cnt 5|sex:not available|strain:T\u00fcpfel long fin TLF", null, null, null, null, null, null, null, null, "Illumina NovaSeq 6000 paired end sequencing; RNA seq of wild type zebrafish embryos developmentally exposed to 3 addictive drugs", "E MTAB 11086:Cnt 5 p", "Cnt 5 p", "RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "Zebrafish embryos were exposed to a drug from xxx dpf ttwo xxx dpf and at the end of the exposure period  larvae were collected as 6 pools of 7 embryos per condition for RNA extraction. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was removed without xxx the beads. Whilst still on the magnet  beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalogue number M0303L. Illumina TruSeq Stranded mRNA Sample Prep Kit.", "Experimental Factor: compound:n1", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP132573", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "ENA FIRST PUBLIC:2022 01 24|ENA LAST UPDATE:2022 01 24", "SLX-19351.G11.HMLG7DRXX.s_2.r_1.fq.gz SLX-19351.G11.HMLG7DRXX.s_2.r_2.fq.gz", "fastq fastq", 1590322800.0, 15903228.0, "E MTAB 11086:SLX 19351.G11.HMLG7DRXX.s 2.r ", "0:50 1:50", "A:407583907;C:385496371;G:392945463;T:404250386;N:46673", 50, 50, null, null, 407583907, 385496371, 392945463, 404250386, 46673, "ERX6700293", "ERS8071617", "ERA6757821", "Cambridge Institute of Therapeutic Immunology & Infectious Disease|European Nucleotide Archive", "Cambridge Institute of Therapeutic Immunology & Infectious Disease|European Nucleotide Archive", 2, 0.94942, 0.95571, 0.10693, 0.10576, 0.69329, 0.69063, 0.46419, 0.4826, 50, 50, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "United Kingdom", "2022-01-24", "Larval", "Larval", "Embryo Imprecise", "All anatomical structures"], [10299, "ERR7132854", "ERX6700292", "ERS8071616", "ERP132573", "PRJEB48231", "RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "E-MTAB-11086", "Transcriptome Analysis", "RNA seq of wild type T\u00fcpfel long fin TLF zebrafish embryos developmentally exposed to three addictive drugs   5 \u00b5M nicotine  1.14 \u00b5M oxycodone and 5 \u00b5M amphetamine. Each of the 24 samples 6 samples per drug  plus 6 control samples represents RNA from a pool of seven 5 dpf zebrafish embryos  exposed to the drug between 1 dpf and 5 dpf.", "ENA FIRST PUBLIC:2022 01 24|ENA LAST UPDATE:2022 01 24", null, "Protocols: Zebrafish embryos were exposed to a drug from xxx dpf ttwo xxx dpf and at the end of the exposure period  larvae were collected as 6 pools of 7 embryos per condition for RNA extraction. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was removed without xxx the beads. Whilst still on the magnet  beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalogue number M0303L. Illumina TruSeq Stranded mRNA Sample Prep Kit.", "Cnt 4", "SAMEA10418772", "Cambridge Institute of Therapeutic Immunology & Infectious Disease", "ENA first public:2022 01 24|ENA last update:2022 01 24|External Id:SAMEA10418772|INSDC center alias:Cambridge Institute of Therapeutic Immunology & Infectious Disease|INSDC center name:Cambridge Institute of Therapeutic Immunology & Infectious Disease|INSDC first public:2022 01 24T16:27:32Z|INSDC last update:2022 01 24T16:27:32Z|INSDC status:public|Submitter Id:E MTAB 11086:Cnt 4|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval day 5|genotype:wild type genotype|individual:pool of 7 embryos|organism part:whole organism|replicate:4|sample name:E MTAB 11086:Cnt 4|sex:not available|strain:T\u00fcpfel long fin TLF", null, null, null, null, null, null, null, null, "Illumina NovaSeq 6000 paired end sequencing; RNA seq of wild type zebrafish embryos developmentally exposed to 3 addictive drugs", "E MTAB 11086:Cnt 4 p", "Cnt 4 p", "RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "Zebrafish embryos were exposed to a drug from xxx dpf ttwo xxx dpf and at the end of the exposure period  larvae were collected as 6 pools of 7 embryos per condition for RNA extraction. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was removed without xxx the beads. Whilst still on the magnet  beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalogue number M0303L. Illumina TruSeq Stranded mRNA Sample Prep Kit.", "Experimental Factor: compound:n1", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP132573", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "ENA FIRST PUBLIC:2022 01 24|ENA LAST UPDATE:2022 01 24", "SLX-19351.C9.HMLG7DRXX.s_2.r_1.fq.gz SLX-19351.C9.HMLG7DRXX.s_2.r_2.fq.gz", "fastq fastq", 2079441500.0, 20794415.0, "E MTAB 11086:SLX 19351.C9.HMLG7DRXX.s 2.r ", "0:50 1:50", "A:555430147;C:483109623;G:489773903;T:551069513;N:58314", 50, 50, null, null, 555430147, 483109623, 489773903, 551069513, 58314, "ERX6700292", "ERS8071616", "ERA6757821", "Cambridge Institute of Therapeutic Immunology & Infectious Disease|European Nucleotide Archive", "Cambridge Institute of Therapeutic Immunology & Infectious Disease|European Nucleotide Archive", 2, 0.94258, 0.9491, 0.10928, 0.10545, 0.66561, 0.66279, 0.46967, 0.47237, 50, 50, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "United Kingdom", "2022-01-24", "Larval", "Larval", "Embryo Imprecise", "All anatomical structures"], [10300, "ERR7132853", "ERX6700291", "ERS8071615", "ERP132573", "PRJEB48231", "RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "E-MTAB-11086", "Transcriptome Analysis", "RNA seq of wild type T\u00fcpfel long fin TLF zebrafish embryos developmentally exposed to three addictive drugs   5 \u00b5M nicotine  1.14 \u00b5M oxycodone and 5 \u00b5M amphetamine. Each of the 24 samples 6 samples per drug  plus 6 control samples represents RNA from a pool of seven 5 dpf zebrafish embryos  exposed to the drug between 1 dpf and 5 dpf.", "ENA FIRST PUBLIC:2022 01 24|ENA LAST UPDATE:2022 01 24", null, "Protocols: Zebrafish embryos were exposed to a drug from xxx dpf ttwo xxx dpf and at the end of the exposure period  larvae were collected as 6 pools of 7 embryos per condition for RNA extraction. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was removed without xxx the beads. Whilst still on the magnet  beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalogue number M0303L. Illumina TruSeq Stranded mRNA Sample Prep Kit.", "Cnt 3", "SAMEA10418771", "Cambridge Institute of Therapeutic Immunology & Infectious Disease", "ENA first public:2022 01 24|ENA last update:2022 01 24|External Id:SAMEA10418771|INSDC center alias:Cambridge Institute of Therapeutic Immunology & Infectious Disease|INSDC center name:Cambridge Institute of Therapeutic Immunology & Infectious Disease|INSDC first public:2022 01 24T16:27:32Z|INSDC last update:2022 01 24T16:27:32Z|INSDC status:public|Submitter Id:E MTAB 11086:Cnt 3|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval day 5|genotype:wild type genotype|individual:pool of 7 embryos|organism part:whole organism|replicate:3|sample name:E MTAB 11086:Cnt 3|sex:not available|strain:T\u00fcpfel long fin TLF", null, null, null, null, null, null, null, null, "Illumina NovaSeq 6000 paired end sequencing; RNA seq of wild type zebrafish embryos developmentally exposed to 3 addictive drugs", "E MTAB 11086:Cnt 3 p", "Cnt 3 p", "RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "Zebrafish embryos were exposed to a drug from xxx dpf ttwo xxx dpf and at the end of the exposure period  larvae were collected as 6 pools of 7 embryos per condition for RNA extraction. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was removed without xxx the beads. Whilst still on the magnet  beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalogue number M0303L. Illumina TruSeq Stranded mRNA Sample Prep Kit.", "Experimental Factor: compound:n1", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP132573", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "ENA FIRST PUBLIC:2022 01 24|ENA LAST UPDATE:2022 01 24", "SLX-19351.H10.HMLG7DRXX.s_2.r_1.fq.gz SLX-19351.H10.HMLG7DRXX.s_2.r_2.fq.gz", "fastq fastq", 2606680900.0, 26066809.0, "E MTAB 11086:SLX 19351.H10.HMLG7DRXX.s 2.r ", "0:50 1:50", "A:698010494;C:604285893;G:612505273;T:691805693;N:73547", 50, 50, null, null, 698010494, 604285893, 612505273, 691805693, 73547, "ERX6700291", "ERS8071615", "ERA6757821", "Cambridge Institute of Therapeutic Immunology & Infectious Disease|European Nucleotide Archive", "Cambridge Institute of Therapeutic Immunology & Infectious Disease|European Nucleotide Archive", 2, 0.94271, 0.94917, 0.11288, 0.10987, 0.67115, 0.66827, 0.4729, 0.4736, 50, 50, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "United Kingdom", "2022-01-24", "Larval", "Larval", "Embryo Imprecise", "All anatomical structures"], [10301, "ERR7132852", "ERX6700290", "ERS8071614", "ERP132573", "PRJEB48231", "RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "E-MTAB-11086", "Transcriptome Analysis", "RNA seq of wild type T\u00fcpfel long fin TLF zebrafish embryos developmentally exposed to three addictive drugs   5 \u00b5M nicotine  1.14 \u00b5M oxycodone and 5 \u00b5M amphetamine. Each of the 24 samples 6 samples per drug  plus 6 control samples represents RNA from a pool of seven 5 dpf zebrafish embryos  exposed to the drug between 1 dpf and 5 dpf.", "ENA FIRST PUBLIC:2022 01 24|ENA LAST UPDATE:2022 01 24", null, "Protocols: Zebrafish embryos were exposed to a drug from xxx dpf ttwo xxx dpf and at the end of the exposure period  larvae were collected as 6 pools of 7 embryos per condition for RNA extraction. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was removed without xxx the beads. Whilst still on the magnet  beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalogue number M0303L. Illumina TruSeq Stranded mRNA Sample Prep Kit.", "Cnt 2", "SAMEA10418770", "Cambridge Institute of Therapeutic Immunology & Infectious Disease", "ENA first public:2022 01 24|ENA last update:2022 01 24|External Id:SAMEA10418770|INSDC center alias:Cambridge Institute of Therapeutic Immunology & Infectious Disease|INSDC center name:Cambridge Institute of Therapeutic Immunology & Infectious Disease|INSDC first public:2022 01 24T16:27:32Z|INSDC last update:2022 01 24T16:27:32Z|INSDC status:public|Submitter Id:E MTAB 11086:Cnt 2|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval day 5|genotype:wild type genotype|individual:pool of 7 embryos|organism part:whole organism|replicate:2|sample name:E MTAB 11086:Cnt 2|sex:not available|strain:T\u00fcpfel long fin TLF", null, null, null, null, null, null, null, null, "Illumina NovaSeq 6000 paired end sequencing; RNA seq of wild type zebrafish embryos developmentally exposed to 3 addictive drugs", "E MTAB 11086:Cnt 2 p", "Cnt 2 p", "RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "Zebrafish embryos were exposed to a drug from xxx dpf ttwo xxx dpf and at the end of the exposure period  larvae were collected as 6 pools of 7 embryos per condition for RNA extraction. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was removed without xxx the beads. Whilst still on the magnet  beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalogue number M0303L. Illumina TruSeq Stranded mRNA Sample Prep Kit.", "Experimental Factor: compound:n1", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP132573", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "ENA FIRST PUBLIC:2022 01 24|ENA LAST UPDATE:2022 01 24", "SLX-19351.E11.HMLG7DRXX.s_2.r_1.fq.gz SLX-19351.E11.HMLG7DRXX.s_2.r_2.fq.gz", "fastq fastq", 2248468400.0, 22484684.0, "E MTAB 11086:SLX 19351.E11.HMLG7DRXX.s 2.r ", "0:50 1:50", "A:601914808;C:521501189;G:527610060;T:597380501;N:61842", 50, 50, null, null, 601914808, 521501189, 527610060, 597380501, 61842, "ERX6700290", "ERS8071614", "ERA6757821", "Cambridge Institute of Therapeutic Immunology & Infectious Disease|European Nucleotide Archive", "Cambridge Institute of Therapeutic Immunology & Infectious Disease|European Nucleotide Archive", 2, 0.94347, 0.94939, 0.11234, 0.10984, 0.66689, 0.66342, 0.46711, 0.46831, 50, 50, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "United Kingdom", "2022-01-24", "Larval", "Larval", "Embryo Imprecise", "All anatomical structures"], [10302, "ERR7132851", "ERX6700289", "ERS8071613", "ERP132573", "PRJEB48231", "RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "E-MTAB-11086", "Transcriptome Analysis", "RNA seq of wild type T\u00fcpfel long fin TLF zebrafish embryos developmentally exposed to three addictive drugs   5 \u00b5M nicotine  1.14 \u00b5M oxycodone and 5 \u00b5M amphetamine. Each of the 24 samples 6 samples per drug  plus 6 control samples represents RNA from a pool of seven 5 dpf zebrafish embryos  exposed to the drug between 1 dpf and 5 dpf.", "ENA FIRST PUBLIC:2022 01 24|ENA LAST UPDATE:2022 01 24", null, "Protocols: Zebrafish embryos were exposed to a drug from xxx dpf ttwo xxx dpf and at the end of the exposure period  larvae were collected as 6 pools of 7 embryos per condition for RNA extraction. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was removed without xxx the beads. Whilst still on the magnet  beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalogue number M0303L. Illumina TruSeq Stranded mRNA Sample Prep Kit.", "Cnt 1", "SAMEA10418769", "Cambridge Institute of Therapeutic Immunology & Infectious Disease", "ENA first public:2022 01 24|ENA last update:2022 01 24|External Id:SAMEA10418769|INSDC center alias:Cambridge Institute of Therapeutic Immunology & Infectious Disease|INSDC center name:Cambridge Institute of Therapeutic Immunology & Infectious Disease|INSDC first public:2022 01 24T16:27:32Z|INSDC last update:2022 01 24T16:27:32Z|INSDC status:public|Submitter Id:E MTAB 11086:Cnt 1|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval day 5|genotype:wild type genotype|individual:pool of 7 embryos|organism part:whole organism|replicate:1|sample name:E MTAB 11086:Cnt 1|sex:not available|strain:T\u00fcpfel long fin TLF", null, null, null, null, null, null, null, null, "Illumina NovaSeq 6000 paired end sequencing; RNA seq of wild type zebrafish embryos developmentally exposed to 3 addictive drugs", "E MTAB 11086:Cnt 1 p", "Cnt 1 p", "RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "Zebrafish embryos were exposed to a drug from xxx dpf ttwo xxx dpf and at the end of the exposure period  larvae were collected as 6 pools of 7 embryos per condition for RNA extraction. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was removed without xxx the beads. Whilst still on the magnet  beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalogue number M0303L. Illumina TruSeq Stranded mRNA Sample Prep Kit.", "Experimental Factor: compound:n1", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP132573", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "ENA FIRST PUBLIC:2022 01 24|ENA LAST UPDATE:2022 01 24", "SLX-19351.D11.HMLG7DRXX.s_2.r_1.fq.gz SLX-19351.D11.HMLG7DRXX.s_2.r_2.fq.gz", "fastq fastq", 1958517300.0, 19585173.0, "E MTAB 11086:SLX 19351.D11.HMLG7DRXX.s 2.r ", "0:50 1:50", "A:523101436;C:454540970;G:459925156;T:520895141;N:54597", 50, 50, null, null, 523101436, 454540970, 459925156, 520895141, 54597, "ERX6700289", "ERS8071613", "ERA6757821", "Cambridge Institute of Therapeutic Immunology & Infectious Disease|European Nucleotide Archive", "Cambridge Institute of Therapeutic Immunology & Infectious Disease|European Nucleotide Archive", 2, 0.94413, 0.95047, 0.10482, 0.10253, 0.67044, 0.66782, 0.47233, 0.4771, 50, 50, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "United Kingdom", "2022-01-24", "Larval", "Larval", "Embryo Imprecise", "All anatomical structures"], [10303, "ERR7132850", "ERX6700288", "ERS8071612", "ERP132573", "PRJEB48231", "RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "E-MTAB-11086", "Transcriptome Analysis", "RNA seq of wild type T\u00fcpfel long fin TLF zebrafish embryos developmentally exposed to three addictive drugs   5 \u00b5M nicotine  1.14 \u00b5M oxycodone and 5 \u00b5M amphetamine. Each of the 24 samples 6 samples per drug  plus 6 control samples represents RNA from a pool of seven 5 dpf zebrafish embryos  exposed to the drug between 1 dpf and 5 dpf.", "ENA FIRST PUBLIC:2022 01 24|ENA LAST UPDATE:2022 01 24", null, "Protocols: Zebrafish embryos were exposed to a drug from xxx dpf ttwo xxx dpf and at the end of the exposure period  larvae were collected as 6 pools of 7 embryos per condition for RNA extraction. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was removed without xxx the beads. Whilst still on the magnet  beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalogue number M0303L. Illumina TruSeq Stranded mRNA Sample Prep Kit.", "Amp 6", "SAMEA10418768", "Cambridge Institute of Therapeutic Immunology & Infectious Disease", "ENA first public:2022 01 24|ENA last update:2022 01 24|External Id:SAMEA10418768|INSDC center alias:Cambridge Institute of Therapeutic Immunology & Infectious Disease|INSDC center name:Cambridge Institute of Therapeutic Immunology & Infectious Disease|INSDC first public:2022 01 24T16:27:32Z|INSDC last update:2022 01 24T16:27:32Z|INSDC status:public|Submitter Id:E MTAB 11086:Amp 6|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval day 5|genotype:wild type genotype|individual:pool of 7 embryos|organism part:whole organism|replicate:6|sample name:E MTAB 11086:Amp 6|sex:not available|strain:T\u00fcpfel long fin TLF", null, null, null, null, null, null, null, null, "Illumina NovaSeq 6000 paired end sequencing; RNA seq of wild type zebrafish embryos developmentally exposed to 3 addictive drugs", "E MTAB 11086:Amp 6 p", "Amp 6 p", "RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "Zebrafish embryos were exposed to a drug from xxx dpf ttwo xxx dpf and at the end of the exposure period  larvae were collected as 6 pools of 7 embryos per condition for RNA extraction. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was removed without xxx the beads. Whilst still on the magnet  beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalogue number M0303L. Illumina TruSeq Stranded mRNA Sample Prep Kit.", "Experimental Factor: compound:amphetamine|Experimental Factor: dose:25", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP132573", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "ENA FIRST PUBLIC:2022 01 24|ENA LAST UPDATE:2022 01 24", "SLX-19351.E9.HMLG7DRXX.s_2.r_1.fq.gz SLX-19351.E9.HMLG7DRXX.s_2.r_2.fq.gz", "fastq fastq", 1712505000.0, 17125050.0, "E MTAB 11086:SLX 19351.E9.HMLG7DRXX.s 2.r ", "0:50 1:50", "A:455641002;C:398314392;G:404466444;T:454036255;N:46907", 50, 50, null, null, 455641002, 398314392, 404466444, 454036255, 46907, "ERX6700288", "ERS8071612", "ERA6757821", "Cambridge Institute of Therapeutic Immunology & Infectious Disease|European Nucleotide Archive", "Cambridge Institute of Therapeutic Immunology & Infectious Disease|European Nucleotide Archive", 2, 0.94358, 0.94966, 0.10681, 0.1037, 0.66665, 0.66373, 0.46688, 0.47225, 50, 50, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "United Kingdom", "2022-01-24", "Larval", "Larval", "Embryo Imprecise", "All anatomical structures"], [10304, "ERR7132849", "ERX6700287", "ERS8071611", "ERP132573", "PRJEB48231", "RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "E-MTAB-11086", "Transcriptome Analysis", "RNA seq of wild type T\u00fcpfel long fin TLF zebrafish embryos developmentally exposed to three addictive drugs   5 \u00b5M nicotine  1.14 \u00b5M oxycodone and 5 \u00b5M amphetamine. Each of the 24 samples 6 samples per drug  plus 6 control samples represents RNA from a pool of seven 5 dpf zebrafish embryos  exposed to the drug between 1 dpf and 5 dpf.", "ENA FIRST PUBLIC:2022 01 24|ENA LAST UPDATE:2022 01 24", null, "Protocols: Zebrafish embryos were exposed to a drug from xxx dpf ttwo xxx dpf and at the end of the exposure period  larvae were collected as 6 pools of 7 embryos per condition for RNA extraction. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was removed without xxx the beads. Whilst still on the magnet  beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalogue number M0303L. Illumina TruSeq Stranded mRNA Sample Prep Kit.", "Amp 5", "SAMEA10418767", "Cambridge Institute of Therapeutic Immunology & Infectious Disease", "ENA first public:2022 01 24|ENA last update:2022 01 24|External Id:SAMEA10418767|INSDC center alias:Cambridge Institute of Therapeutic Immunology & Infectious Disease|INSDC center name:Cambridge Institute of Therapeutic Immunology & Infectious Disease|INSDC first public:2022 01 24T16:27:32Z|INSDC last update:2022 01 24T16:27:32Z|INSDC status:public|Submitter Id:E MTAB 11086:Amp 5|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval day 5|genotype:wild type genotype|individual:pool of 7 embryos|organism part:whole organism|replicate:5|sample name:E MTAB 11086:Amp 5|sex:not available|strain:T\u00fcpfel long fin TLF", null, null, null, null, null, null, null, null, "Illumina NovaSeq 6000 paired end sequencing; RNA seq of wild type zebrafish embryos developmentally exposed to 3 addictive drugs", "E MTAB 11086:Amp 5 p", "Amp 5 p", "RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "Zebrafish embryos were exposed to a drug from xxx dpf ttwo xxx dpf and at the end of the exposure period  larvae were collected as 6 pools of 7 embryos per condition for RNA extraction. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was removed without xxx the beads. Whilst still on the magnet  beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalogue number M0303L. Illumina TruSeq Stranded mRNA Sample Prep Kit.", "Experimental Factor: compound:amphetamine|Experimental Factor: dose:25", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP132573", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "ENA FIRST PUBLIC:2022 01 24|ENA LAST UPDATE:2022 01 24", "SLX-19351.A10.HMLG7DRXX.s_2.r_1.fq.gz SLX-19351.A10.HMLG7DRXX.s_2.r_2.fq.gz", "fastq fastq", 2007519200.0, 20075192.0, "E MTAB 11086:SLX 19351.A10.HMLG7DRXX.s 2.r ", "0:50 1:50", "A:534568558;C:467539630;G:473805079;T:531550688;N:55245", 50, 50, null, null, 534568558, 467539630, 473805079, 531550688, 55245, "ERX6700287", "ERS8071611", "ERA6757821", "Cambridge Institute of Therapeutic Immunology & Infectious Disease|European Nucleotide Archive", "Cambridge Institute of Therapeutic Immunology & Infectious Disease|European Nucleotide Archive", 2, 0.94408, 0.9499, 0.10888, 0.10604, 0.66797, 0.66458, 0.46039, 0.46669, 50, 50, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "United Kingdom", "2022-01-24", "Larval", "Larval", "Embryo Imprecise", "All anatomical structures"], [10305, "ERR7132848", "ERX6700286", "ERS8071610", "ERP132573", "PRJEB48231", "RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "E-MTAB-11086", "Transcriptome Analysis", "RNA seq of wild type T\u00fcpfel long fin TLF zebrafish embryos developmentally exposed to three addictive drugs   5 \u00b5M nicotine  1.14 \u00b5M oxycodone and 5 \u00b5M amphetamine. Each of the 24 samples 6 samples per drug  plus 6 control samples represents RNA from a pool of seven 5 dpf zebrafish embryos  exposed to the drug between 1 dpf and 5 dpf.", "ENA FIRST PUBLIC:2022 01 24|ENA LAST UPDATE:2022 01 24", null, "Protocols: Zebrafish embryos were exposed to a drug from xxx dpf ttwo xxx dpf and at the end of the exposure period  larvae were collected as 6 pools of 7 embryos per condition for RNA extraction. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was removed without xxx the beads. Whilst still on the magnet  beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalogue number M0303L. Illumina TruSeq Stranded mRNA Sample Prep Kit.", "Amp 4", "SAMEA10418766", "Cambridge Institute of Therapeutic Immunology & Infectious Disease", "ENA first public:2022 01 24|ENA last update:2022 01 24|External Id:SAMEA10418766|INSDC center alias:Cambridge Institute of Therapeutic Immunology & Infectious Disease|INSDC center name:Cambridge Institute of Therapeutic Immunology & Infectious Disease|INSDC first public:2022 01 24T16:27:32Z|INSDC last update:2022 01 24T16:27:32Z|INSDC status:public|Submitter Id:E MTAB 11086:Amp 4|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval day 5|genotype:wild type genotype|individual:pool of 7 embryos|organism part:whole organism|replicate:4|sample name:E MTAB 11086:Amp 4|sex:not available|strain:T\u00fcpfel long fin TLF", null, null, null, null, null, null, null, null, "Illumina NovaSeq 6000 paired end sequencing; RNA seq of wild type zebrafish embryos developmentally exposed to 3 addictive drugs", "E MTAB 11086:Amp 4 p", "Amp 4 p", "RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "Zebrafish embryos were exposed to a drug from xxx dpf ttwo xxx dpf and at the end of the exposure period  larvae were collected as 6 pools of 7 embryos per condition for RNA extraction. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was removed without xxx the beads. Whilst still on the magnet  beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalogue number M0303L. Illumina TruSeq Stranded mRNA Sample Prep Kit.", "Experimental Factor: compound:amphetamine|Experimental Factor: dose:25", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP132573", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "ENA FIRST PUBLIC:2022 01 24|ENA LAST UPDATE:2022 01 24", "SLX-19351.F11.HMLG7DRXX.s_2.r_1.fq.gz SLX-19351.F11.HMLG7DRXX.s_2.r_2.fq.gz", "fastq fastq", 1845345300.0, 18453453.0, "E MTAB 11086:SLX 19351.F11.HMLG7DRXX.s 2.r ", "0:50 1:50", "A:490920758;C:430637266;G:435667744;T:488067453;N:52079", 50, 50, null, null, 490920758, 430637266, 435667744, 488067453, 52079, "ERX6700286", "ERS8071610", "ERA6757821", "Cambridge Institute of Therapeutic Immunology & Infectious Disease|European Nucleotide Archive", "Cambridge Institute of Therapeutic Immunology & Infectious Disease|European Nucleotide Archive", 2, 0.94484, 0.9504, 0.10472, 0.10237, 0.66618, 0.66336, 0.46903, 0.47774, 50, 50, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "United Kingdom", "2022-01-24", "Larval", "Larval", "Embryo Imprecise", "All anatomical structures"], [10306, "ERR7132847", "ERX6700285", "ERS8071609", "ERP132573", "PRJEB48231", "RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "E-MTAB-11086", "Transcriptome Analysis", "RNA seq of wild type T\u00fcpfel long fin TLF zebrafish embryos developmentally exposed to three addictive drugs   5 \u00b5M nicotine  1.14 \u00b5M oxycodone and 5 \u00b5M amphetamine. Each of the 24 samples 6 samples per drug  plus 6 control samples represents RNA from a pool of seven 5 dpf zebrafish embryos  exposed to the drug between 1 dpf and 5 dpf.", "ENA FIRST PUBLIC:2022 01 24|ENA LAST UPDATE:2022 01 24", null, "Protocols: Zebrafish embryos were exposed to a drug from xxx dpf ttwo xxx dpf and at the end of the exposure period  larvae were collected as 6 pools of 7 embryos per condition for RNA extraction. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was removed without xxx the beads. Whilst still on the magnet  beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalogue number M0303L. Illumina TruSeq Stranded mRNA Sample Prep Kit.", "Amp 3", "SAMEA10418765", "Cambridge Institute of Therapeutic Immunology & Infectious Disease", "ENA first public:2022 01 24|ENA last update:2022 01 24|External Id:SAMEA10418765|INSDC center alias:Cambridge Institute of Therapeutic Immunology & Infectious Disease|INSDC center name:Cambridge Institute of Therapeutic Immunology & Infectious Disease|INSDC first public:2022 01 24T16:27:32Z|INSDC last update:2022 01 24T16:27:32Z|INSDC status:public|Submitter Id:E MTAB 11086:Amp 3|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval day 5|genotype:wild type genotype|individual:pool of 7 embryos|organism part:whole organism|replicate:3|sample name:E MTAB 11086:Amp 3|sex:not available|strain:T\u00fcpfel long fin TLF", null, null, null, null, null, null, null, null, "Illumina NovaSeq 6000 paired end sequencing; RNA seq of wild type zebrafish embryos developmentally exposed to 3 addictive drugs", "E MTAB 11086:Amp 3 p", "Amp 3 p", "RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "Zebrafish embryos were exposed to a drug from xxx dpf ttwo xxx dpf and at the end of the exposure period  larvae were collected as 6 pools of 7 embryos per condition for RNA extraction. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was removed without xxx the beads. Whilst still on the magnet  beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalogue number M0303L. Illumina TruSeq Stranded mRNA Sample Prep Kit.", "Experimental Factor: compound:amphetamine|Experimental Factor: dose:25", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP132573", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "ENA FIRST PUBLIC:2022 01 24|ENA LAST UPDATE:2022 01 24", "SLX-19351.F9.HMLG7DRXX.s_2.r_1.fq.gz SLX-19351.F9.HMLG7DRXX.s_2.r_2.fq.gz", "fastq fastq", 2195415300.0, 21954153.0, "E MTAB 11086:SLX 19351.F9.HMLG7DRXX.s 2.r ", "0:50 1:50", "A:583956682;C:511927452;G:518373455;T:581095379;N:62332", 50, 50, null, null, 583956682, 511927452, 518373455, 581095379, 62332, "ERX6700285", "ERS8071609", "ERA6757821", "Cambridge Institute of Therapeutic Immunology & Infectious Disease|European Nucleotide Archive", "Cambridge Institute of Therapeutic Immunology & Infectious Disease|European Nucleotide Archive", 2, 0.94571, 0.95178, 0.10567, 0.10288, 0.6688, 0.66661, 0.4677, 0.47288, 50, 50, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "United Kingdom", "2022-01-24", "Larval", "Larval", "Embryo Imprecise", "All anatomical structures"], [10307, "ERR7132846", "ERX6700284", "ERS8071608", "ERP132573", "PRJEB48231", "RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "E-MTAB-11086", "Transcriptome Analysis", "RNA seq of wild type T\u00fcpfel long fin TLF zebrafish embryos developmentally exposed to three addictive drugs   5 \u00b5M nicotine  1.14 \u00b5M oxycodone and 5 \u00b5M amphetamine. Each of the 24 samples 6 samples per drug  plus 6 control samples represents RNA from a pool of seven 5 dpf zebrafish embryos  exposed to the drug between 1 dpf and 5 dpf.", "ENA FIRST PUBLIC:2022 01 24|ENA LAST UPDATE:2022 01 24", null, "Protocols: Zebrafish embryos were exposed to a drug from xxx dpf ttwo xxx dpf and at the end of the exposure period  larvae were collected as 6 pools of 7 embryos per condition for RNA extraction. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was removed without xxx the beads. Whilst still on the magnet  beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalogue number M0303L. Illumina TruSeq Stranded mRNA Sample Prep Kit.", "Amp 2", "SAMEA10418764", "Cambridge Institute of Therapeutic Immunology & Infectious Disease", "ENA first public:2022 01 24|ENA last update:2022 01 24|External Id:SAMEA10418764|INSDC center alias:Cambridge Institute of Therapeutic Immunology & Infectious Disease|INSDC center name:Cambridge Institute of Therapeutic Immunology & Infectious Disease|INSDC first public:2022 01 24T16:27:32Z|INSDC last update:2022 01 24T16:27:32Z|INSDC status:public|Submitter Id:E MTAB 11086:Amp 2|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval day 5|genotype:wild type genotype|individual:pool of 7 embryos|organism part:whole organism|replicate:2|sample name:E MTAB 11086:Amp 2|sex:not available|strain:T\u00fcpfel long fin TLF", null, null, null, null, null, null, null, null, "Illumina NovaSeq 6000 paired end sequencing; RNA seq of wild type zebrafish embryos developmentally exposed to 3 addictive drugs", "E MTAB 11086:Amp 2 p", "Amp 2 p", "RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "Zebrafish embryos were exposed to a drug from xxx dpf ttwo xxx dpf and at the end of the exposure period  larvae were collected as 6 pools of 7 embryos per condition for RNA extraction. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was removed without xxx the beads. Whilst still on the magnet  beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalogue number M0303L. Illumina TruSeq Stranded mRNA Sample Prep Kit.", "Experimental Factor: compound:amphetamine|Experimental Factor: dose:25", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP132573", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "ENA FIRST PUBLIC:2022 01 24|ENA LAST UPDATE:2022 01 24", "SLX-19351.D9.HMLG7DRXX.s_2.r_1.fq.gz SLX-19351.D9.HMLG7DRXX.s_2.r_2.fq.gz", "fastq fastq", 2093635000.0, 20936350.0, "E MTAB 11086:SLX 19351.D9.HMLG7DRXX.s 2.r ", "0:50 1:50", "A:556451381;C:488314672;G:495691630;T:553118514;N:58803", 50, 50, null, null, 556451381, 488314672, 495691630, 553118514, 58803, "ERX6700284", "ERS8071608", "ERA6757821", "Cambridge Institute of Therapeutic Immunology & Infectious Disease|European Nucleotide Archive", "Cambridge Institute of Therapeutic Immunology & Infectious Disease|European Nucleotide Archive", 2, 0.94407, 0.95012, 0.11198, 0.10872, 0.66651, 0.66352, 0.46901, 0.47198, 50, 50, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "United Kingdom", "2022-01-24", "Larval", "Larval", "Embryo Imprecise", "All anatomical structures"], [10308, "ERR7132845", "ERX6700283", "ERS8071607", "ERP132573", "PRJEB48231", "RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "E-MTAB-11086", "Transcriptome Analysis", "RNA seq of wild type T\u00fcpfel long fin TLF zebrafish embryos developmentally exposed to three addictive drugs   5 \u00b5M nicotine  1.14 \u00b5M oxycodone and 5 \u00b5M amphetamine. Each of the 24 samples 6 samples per drug  plus 6 control samples represents RNA from a pool of seven 5 dpf zebrafish embryos  exposed to the drug between 1 dpf and 5 dpf.", "ENA FIRST PUBLIC:2022 01 24|ENA LAST UPDATE:2022 01 24", null, "Protocols: Zebrafish embryos were exposed to a drug from xxx dpf ttwo xxx dpf and at the end of the exposure period  larvae were collected as 6 pools of 7 embryos per condition for RNA extraction. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was removed without xxx the beads. Whilst still on the magnet  beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalogue number M0303L. Illumina TruSeq Stranded mRNA Sample Prep Kit.", "Amp 1", "SAMEA10418763", "Cambridge Institute of Therapeutic Immunology & Infectious Disease", "ENA first public:2022 01 24|ENA last update:2022 01 24|External Id:SAMEA10418763|INSDC center alias:Cambridge Institute of Therapeutic Immunology & Infectious Disease|INSDC center name:Cambridge Institute of Therapeutic Immunology & Infectious Disease|INSDC first public:2022 01 24T16:27:32Z|INSDC last update:2022 01 24T16:27:32Z|INSDC status:public|Submitter Id:E MTAB 11086:Amp 1|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:larval day 5|genotype:wild type genotype|individual:pool of 7 embryos|organism part:whole organism|replicate:1|sample name:E MTAB 11086:Amp 1|sex:not available|strain:T\u00fcpfel long fin TLF", null, null, null, null, null, null, null, null, "Illumina NovaSeq 6000 paired end sequencing; RNA seq of wild type zebrafish embryos developmentally exposed to 3 addictive drugs", "E MTAB 11086:Amp 1 p", "Amp 1 p", "RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "Zebrafish embryos were exposed to a drug from xxx dpf ttwo xxx dpf and at the end of the exposure period  larvae were collected as 6 pools of 7 embryos per condition for RNA extraction. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was removed without xxx the beads. Whilst still on the magnet  beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalogue number M0303L. Illumina TruSeq Stranded mRNA Sample Prep Kit.", "Experimental Factor: compound:amphetamine|Experimental Factor: dose:25", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP132573", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of wild type zebrafish embryos developmentally exposed to three addictive drugs", "ENA FIRST PUBLIC:2022 01 24|ENA LAST UPDATE:2022 01 24", "SLX-19351.E10.HMLG7DRXX.s_2.r_1.fq.gz SLX-19351.E10.HMLG7DRXX.s_2.r_2.fq.gz", "fastq fastq", 2254130000.0, 22541300.0, "E MTAB 11086:SLX 19351.E10.HMLG7DRXX.s 2.r ", "0:50 1:50", "A:599784010;C:525655199;G:531849347;T:596777643;N:63801", 50, 50, null, null, 599784010, 525655199, 531849347, 596777643, 63801, "ERX6700283", "ERS8071607", "ERA6757821", "Cambridge Institute of Therapeutic Immunology & Infectious Disease|European Nucleotide Archive", "Cambridge Institute of Therapeutic Immunology & Infectious Disease|European Nucleotide Archive", 2, 0.94449, 0.95026, 0.10608, 0.10323, 0.66758, 0.66257, 0.46869, 0.47045, 50, 50, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "United Kingdom", "2022-01-24", "Larval", "Larval", "Embryo Imprecise", "All anatomical structures"], [11245, "ERR10851251", "ERX10296231", "ERS14601258", "ERP144652", "PRJEB59599", "Heterozygous Gata2b versus wildtype Gata2b zebrafish embryo HSPC CD41", "E-MTAB-12577", "Transcriptome Analysis", "Comparison between wildtype and Gata2b heterozygous zebrafish HSPCs", "ENA FIRST PUBLIC:2023 02 17|ENA LAST UPDATE:2023 02 17", null, "Protocols: Embryos were dissociated using Collagenase 1  2 and 4 and sorted using a FACS Aria III in trizol RNA was isolated from trizol RNA was smarter amplified", "WT3 cd41pflt1p48hpf", "SAMEA112483908", "Department of Hematology cancer institute ErasmusMC", "ENA FIRST PUBLIC:2023 02 17|ENA LAST UPDATE:2023 02 17|External Id:SAMEA112483908|INSDC center alias:Department of Hematology cancer institute ErasmusMC|INSDC center name:Department of Hematology cancer institute ErasmusMC|INSDC first public:2023 02 17T00:21:53Z|INSDC last update:2023 02 17T00:21:53Z|INSDC status:public|Submitter Id:E MTAB 12577:WT3 cd41pflt1p48hpf|age:48|broker name:ArrayExpress|cell type:hematopoietic stem cell|common name:zebrafish|developmental stage:embryo|genotype:wild type genotype|immunophenotype:CD41:GFP+ Flt1:RFP+|individual:pools of 3 embryos 1|organism part:liver|sample name:E MTAB 12577:WT3 cd41pflt1p48hpf|scientific name:Danio rerio|strain:TgCD41:GFP TgFlt1:RFP WT", null, null, null, null, null, null, null, null, "Illumina NovaSeq 6000 paired end sequencing; Heterozygous Gata2b versus wildtype Gata2b zebrafish embryo HSPC CD41", "E MTAB 12577:WT3 cd41pflt1p48hpf p", "WT3 cd41pflt1p48hpf p", "Heterozygous Gata2b versus wildtype Gata2b zebrafish embryo HSPC CD41", "Embryos were dissociated using Collagenase 1  2 and 4 and sorted using a FACS Aria III in trizol RNA was isolated from trizol RNA was smarter amplified", "Experimental Factor: genotype:wild type genotype", "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP144652", "Illumina NovaSeq 6000 paired end sequencing; Heterozygous Gata2b versus wildtype Gata2b zebrafish embryo HSPC CD41", "ENA FIRST PUBLIC:2023 02 17|ENA LAST UPDATE:2023 02 17", "WT3_cd41pflt1p48hpf_R1.fastq.gz WT3_cd41pflt1p48hpf_R2.fastq.gz", "fastq fastq", 2865896816.0, 14187608.0, "E MTAB 12577:WT3 cd41pflt1p48hpf R", "0:101 1:101", "A:766433553;C:631932872;G:660672891;T:806849737;N:7763", 101, 101, null, null, 766433553, 631932872, 660672891, 806849737, 7763, "ERX10296231", "ERS14601258", "ERA20429817", "Department of Hematology cancer institute ErasmusMC|European Nucleotide Archive", "Department of Hematology cancer institute ErasmusMC|European Nucleotide Archive", 2, 0.90501, 0.85042, 0.09405, 0.09335, 0.87286, 0.87943, 0.48423, 0.47904, 101, 101, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "full_length", "cdna_unspecified", "smarter", "bulk", "unknown", "unknown", null, "Netherlands", "2023-02-17", "Hatching", "Embryo", "Embryo Imprecise", "All anatomical structures"], [11246, "ERR10851250", "ERX10296230", "ERS14601257", "ERP144652", "PRJEB59599", "Heterozygous Gata2b versus wildtype Gata2b zebrafish embryo HSPC CD41", "E-MTAB-12577", "Transcriptome Analysis", "Comparison between wildtype and Gata2b heterozygous zebrafish HSPCs", "ENA FIRST PUBLIC:2023 02 17|ENA LAST UPDATE:2023 02 17", null, "Protocols: Embryos were dissociated using Collagenase 1  2 and 4 and sorted using a FACS Aria III in trizol RNA was isolated from trizol RNA was smarter amplified", "WT2 cd41pflt1p48hpf", "SAMEA112483907", "Department of Hematology cancer institute ErasmusMC", "ENA FIRST PUBLIC:2023 02 17|ENA LAST UPDATE:2023 02 17|External Id:SAMEA112483907|INSDC center alias:Department of Hematology cancer institute ErasmusMC|INSDC center name:Department of Hematology cancer institute ErasmusMC|INSDC first public:2023 02 17T00:21:53Z|INSDC last update:2023 02 17T00:21:53Z|INSDC status:public|Submitter Id:E MTAB 12577:WT2 cd41pflt1p48hpf|age:48|broker name:ArrayExpress|cell type:hematopoietic stem cell|common name:zebrafish|developmental stage:embryo|genotype:wild type genotype|immunophenotype:CD41:GFP+ Flt1:RFP+|individual:pools of 3 embryos 5|organism part:liver|sample name:E MTAB 12577:WT2 cd41pflt1p48hpf|scientific name:Danio rerio|strain:TgCD41:GFP TgFlt1:RFP WT", null, null, null, null, null, null, null, null, "Illumina NovaSeq 6000 paired end sequencing; Heterozygous Gata2b versus wildtype Gata2b zebrafish embryo HSPC CD41", "E MTAB 12577:WT2 cd41pflt1p48hpf p", "WT2 cd41pflt1p48hpf p", "Heterozygous Gata2b versus wildtype Gata2b zebrafish embryo HSPC CD41", "Embryos were dissociated using Collagenase 1  2 and 4 and sorted using a FACS Aria III in trizol RNA was isolated from trizol RNA was smarter amplified", "Experimental Factor: genotype:wild type genotype", "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP144652", "Illumina NovaSeq 6000 paired end sequencing; Heterozygous Gata2b versus wildtype Gata2b zebrafish embryo HSPC CD41", "ENA FIRST PUBLIC:2023 02 17|ENA LAST UPDATE:2023 02 17", "WT2_cd41pflt1p48hpf_R1.fastq.gz WT2_cd41pflt1p48hpf_R2.fastq.gz", "fastq fastq", 2929743360.0, 14503680.0, "E MTAB 12577:WT2 cd41pflt1p48hpf R", "0:101 1:101", "A:803775344;C:639485652;G:661899077;T:824575679;N:7608", 101, 101, null, null, 803775344, 639485652, 661899077, 824575679, 7608, "ERX10296230", "ERS14601257", "ERA20429817", "Department of Hematology cancer institute ErasmusMC|European Nucleotide Archive", "Department of Hematology cancer institute ErasmusMC|European Nucleotide Archive", 2, 0.94558, 0.90768, 0.09379, 0.09399, 0.79058, 0.79833, 0.48216, 0.47743, 101, 101, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "full_length", "cdna_unspecified", "smarter", "bulk", "unknown", "unknown", null, "Netherlands", "2023-02-17", "Hatching", "Embryo", "Embryo Imprecise", "All anatomical structures"], [11247, "ERR10851249", "ERX10296229", "ERS14601256", "ERP144652", "PRJEB59599", "Heterozygous Gata2b versus wildtype Gata2b zebrafish embryo HSPC CD41", "E-MTAB-12577", "Transcriptome Analysis", "Comparison between wildtype and Gata2b heterozygous zebrafish HSPCs", "ENA FIRST PUBLIC:2023 02 17|ENA LAST UPDATE:2023 02 17", null, "Protocols: Embryos were dissociated using Collagenase 1  2 and 4 and sorted using a FACS Aria III in trizol RNA was isolated from trizol RNA was smarter amplified", "WT1 cd41pflt1p48hpf", "SAMEA112483906", "Department of Hematology cancer institute ErasmusMC", "ENA FIRST PUBLIC:2023 02 17|ENA LAST UPDATE:2023 02 17|External Id:SAMEA112483906|INSDC center alias:Department of Hematology cancer institute ErasmusMC|INSDC center name:Department of Hematology cancer institute ErasmusMC|INSDC first public:2023 02 17T00:21:53Z|INSDC last update:2023 02 17T00:21:53Z|INSDC status:public|Submitter Id:E MTAB 12577:WT1 cd41pflt1p48hpf|age:48|broker name:ArrayExpress|cell type:hematopoietic stem cell|common name:zebrafish|developmental stage:embryo|genotype:wild type genotype|immunophenotype:CD41:GFP+ Flt1:RFP+|individual:pools of 3 embryos 4|organism part:liver|sample name:E MTAB 12577:WT1 cd41pflt1p48hpf|scientific name:Danio rerio|strain:TgCD41:GFP TgFlt1:RFP WT", null, null, null, null, null, null, null, null, "Illumina NovaSeq 6000 paired end sequencing; Heterozygous Gata2b versus wildtype Gata2b zebrafish embryo HSPC CD41", "E MTAB 12577:WT1 cd41pflt1p48hpf p", "WT1 cd41pflt1p48hpf p", "Heterozygous Gata2b versus wildtype Gata2b zebrafish embryo HSPC CD41", "Embryos were dissociated using Collagenase 1  2 and 4 and sorted using a FACS Aria III in trizol RNA was isolated from trizol RNA was smarter amplified", "Experimental Factor: genotype:wild type genotype", "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP144652", "Illumina NovaSeq 6000 paired end sequencing; Heterozygous Gata2b versus wildtype Gata2b zebrafish embryo HSPC CD41", "ENA FIRST PUBLIC:2023 02 17|ENA LAST UPDATE:2023 02 17", "WT1_cd41pflt1p48hpf_R1.fastq.gz WT1_cd41pflt1p48hpf_R2.fastq.gz", "fastq fastq", 3248647426.0, 16082413.0, "E MTAB 12577:WT1 cd41pflt1p48hpf R", "0:101 1:101", "A:883030193;C:725566673;G:750551574;T:889489774;N:9212", 101, 101, null, null, 883030193, 725566673, 750551574, 889489774, 9212, "ERX10296229", "ERS14601256", "ERA20429817", "Department of Hematology cancer institute ErasmusMC|European Nucleotide Archive", "Department of Hematology cancer institute ErasmusMC|European Nucleotide Archive", 2, 0.91568, 0.8915, 0.09819, 0.09602, 0.86634, 0.8706, 0.48887, 0.48731, 101, 101, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "full_length", "cdna_unspecified", "smarter", "bulk", "unknown", "unknown", null, "Netherlands", "2023-02-17", "Hatching", "Embryo", "Embryo Imprecise", "All anatomical structures"], [11248, "ERR10851248", "ERX10296228", "ERS14601255", "ERP144652", "PRJEB59599", "Heterozygous Gata2b versus wildtype Gata2b zebrafish embryo HSPC CD41", "E-MTAB-12577", "Transcriptome Analysis", "Comparison between wildtype and Gata2b heterozygous zebrafish HSPCs", "ENA FIRST PUBLIC:2023 02 17|ENA LAST UPDATE:2023 02 17", null, "Protocols: Embryos were dissociated using Collagenase 1  2 and 4 and sorted using a FACS Aria III in trizol RNA was isolated from trizol RNA was smarter amplified", "Het3 cd41pflt1p48hpf", "SAMEA112483905", "Department of Hematology cancer institute ErasmusMC", "ENA FIRST PUBLIC:2023 02 17|ENA LAST UPDATE:2023 02 17|External Id:SAMEA112483905|INSDC center alias:Department of Hematology cancer institute ErasmusMC|INSDC center name:Department of Hematology cancer institute ErasmusMC|INSDC first public:2023 02 17T00:21:53Z|INSDC last update:2023 02 17T00:21:53Z|INSDC status:public|Submitter Id:E MTAB 12577:Het3 cd41pflt1p48hpf|age:48|broker name:ArrayExpress|cell type:hematopoietic stem cell|common name:zebrafish|developmental stage:embryo|genotype:Gata2b +/ |immunophenotype:CD41:GFP+ Flt1:RFP+|individual:pools of 3 embryos 3|organism part:liver|sample name:E MTAB 12577:Het3 cd41pflt1p48hpf|scientific name:Danio rerio|strain:TgCD41:GFP TgFlt1:RFP Gata2b KO", null, null, null, null, null, null, null, null, "Illumina NovaSeq 6000 paired end sequencing; Heterozygous Gata2b versus wildtype Gata2b zebrafish embryo HSPC CD41", "E MTAB 12577:Het3 cd41pflt1p48hpf p", "Het3 cd41pflt1p48hpf p", "Heterozygous Gata2b versus wildtype Gata2b zebrafish embryo HSPC CD41", "Embryos were dissociated using Collagenase 1  2 and 4 and sorted using a FACS Aria III in trizol RNA was isolated from trizol RNA was smarter amplified", "Experimental Factor: genotype:Gata2b +/ ", "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP144652", "Illumina NovaSeq 6000 paired end sequencing; Heterozygous Gata2b versus wildtype Gata2b zebrafish embryo HSPC CD41", "ENA FIRST PUBLIC:2023 02 17|ENA LAST UPDATE:2023 02 17", "Het3_cd41pflt1p48hpf_R1.fastq.gz Het3_cd41pflt1p48hpf_R2.fastq.gz", "fastq fastq", 2826011512.0, 13990156.0, "E MTAB 12577:Het3 cd41pflt1p48hpf R", "0:101 1:101", "A:777245323;C:622387331;G:640520737;T:785850577;N:7544", 101, 101, null, null, 777245323, 622387331, 640520737, 785850577, 7544, "ERX10296228", "ERS14601255", "ERA20429817", "Department of Hematology cancer institute ErasmusMC|European Nucleotide Archive", "Department of Hematology cancer institute ErasmusMC|European Nucleotide Archive", 2, 0.92716, 0.90121, 0.10896, 0.10895, 0.76739, 0.77628, 0.47007, 0.46569, 101, 101, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "full_length", "cdna_unspecified", "smarter", "bulk", "unknown", "unknown", null, "Netherlands", "2023-02-17", "Hatching", "Embryo", "Embryo Imprecise", "All anatomical structures"], [11249, "ERR10851247", "ERX10296227", "ERS14601254", "ERP144652", "PRJEB59599", "Heterozygous Gata2b versus wildtype Gata2b zebrafish embryo HSPC CD41", "E-MTAB-12577", "Transcriptome Analysis", "Comparison between wildtype and Gata2b heterozygous zebrafish HSPCs", "ENA FIRST PUBLIC:2023 02 17|ENA LAST UPDATE:2023 02 17", null, "Protocols: Embryos were dissociated using Collagenase 1  2 and 4 and sorted using a FACS Aria III in trizol RNA was isolated from trizol RNA was smarter amplified", "Het2 cd41pflt1p48hpf", "SAMEA112483904", "Department of Hematology cancer institute ErasmusMC", "ENA FIRST PUBLIC:2023 02 17|ENA LAST UPDATE:2023 02 17|External Id:SAMEA112483904|INSDC center alias:Department of Hematology cancer institute ErasmusMC|INSDC center name:Department of Hematology cancer institute ErasmusMC|INSDC first public:2023 02 17T00:21:53Z|INSDC last update:2023 02 17T00:21:53Z|INSDC status:public|Submitter Id:E MTAB 12577:Het2 cd41pflt1p48hpf|age:48|broker name:ArrayExpress|cell type:hematopoietic stem cell|common name:zebrafish|developmental stage:embryo|genotype:Gata2b +/ |immunophenotype:CD41:GFP+ Flt1:RFP+|individual:pools of 3 embryos 2|organism part:liver|sample name:E MTAB 12577:Het2 cd41pflt1p48hpf|scientific name:Danio rerio|strain:TgCD41:GFP TgFlt1:RFP Gata2b KO", null, null, null, null, null, null, null, null, "Illumina NovaSeq 6000 paired end sequencing; Heterozygous Gata2b versus wildtype Gata2b zebrafish embryo HSPC CD41", "E MTAB 12577:Het2 cd41pflt1p48hpf p", "Het2 cd41pflt1p48hpf p", "Heterozygous Gata2b versus wildtype Gata2b zebrafish embryo HSPC CD41", "Embryos were dissociated using Collagenase 1  2 and 4 and sorted using a FACS Aria III in trizol RNA was isolated from trizol RNA was smarter amplified", "Experimental Factor: genotype:Gata2b +/ ", "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP144652", "Illumina NovaSeq 6000 paired end sequencing; Heterozygous Gata2b versus wildtype Gata2b zebrafish embryo HSPC CD41", "ENA FIRST PUBLIC:2023 02 17|ENA LAST UPDATE:2023 02 17", "Het2_cd41pflt1p48hpf_R1.fastq.gz Het2_cd41pflt1p48hpf_R2.fastq.gz", "fastq fastq", 2999149954.0, 14847277.0, "E MTAB 12577:Het2 cd41pflt1p48hpf R", "0:101 1:101", "A:822247269;C:656610849;G:681974696;T:838308917;N:8223", 101, 101, null, null, 822247269, 656610849, 681974696, 838308917, 8223, "ERX10296227", "ERS14601254", "ERA20429817", "Department of Hematology cancer institute ErasmusMC|European Nucleotide Archive", "Department of Hematology cancer institute ErasmusMC|European Nucleotide Archive", 2, 0.94678, 0.91473, 0.10277, 0.10053, 0.76686, 0.77684, 0.46042, 0.47202, 101, 101, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "full_length", "cdna_unspecified", "smarter", "bulk", "unknown", "unknown", null, "Netherlands", "2023-02-17", "Hatching", "Embryo", "Embryo Imprecise", "All anatomical structures"], [11250, "ERR10851246", "ERX10296226", "ERS14601253", "ERP144652", "PRJEB59599", "Heterozygous Gata2b versus wildtype Gata2b zebrafish embryo HSPC CD41", "E-MTAB-12577", "Transcriptome Analysis", "Comparison between wildtype and Gata2b heterozygous zebrafish HSPCs", "ENA FIRST PUBLIC:2023 02 17|ENA LAST UPDATE:2023 02 17", null, "Protocols: Embryos were dissociated using Collagenase 1  2 and 4 and sorted using a FACS Aria III in trizol RNA was isolated from trizol RNA was smarter amplified", "Het1 cd41pflt1p48hpf", "SAMEA112483903", "Department of Hematology cancer institute ErasmusMC", "ENA FIRST PUBLIC:2023 02 17|ENA LAST UPDATE:2023 02 17|External Id:SAMEA112483903|INSDC center alias:Department of Hematology cancer institute ErasmusMC|INSDC center name:Department of Hematology cancer institute ErasmusMC|INSDC first public:2023 02 17T00:21:53Z|INSDC last update:2023 02 17T00:21:53Z|INSDC status:public|Submitter Id:E MTAB 12577:Het1 cd41pflt1p48hpf|age:48|broker name:ArrayExpress|cell type:hematopoietic stem cell|common name:zebrafish|developmental stage:embryo|genotype:Gata2b +/ |immunophenotype:CD41:GFP+ Flt1:RFP+|individual:pools of 3 embryos 1|organism part:liver|sample name:E MTAB 12577:Het1 cd41pflt1p48hpf|scientific name:Danio rerio|strain:TgCD41:GFP TgFlt1:RFP Gata2b KO", null, null, null, null, null, null, null, null, "Illumina NovaSeq 6000 paired end sequencing; Heterozygous Gata2b versus wildtype Gata2b zebrafish embryo HSPC CD41", "E MTAB 12577:Het1 cd41pflt1p48hpf p", "Het1 cd41pflt1p48hpf p", "Heterozygous Gata2b versus wildtype Gata2b zebrafish embryo HSPC CD41", "Embryos were dissociated using Collagenase 1  2 and 4 and sorted using a FACS Aria III in trizol RNA was isolated from trizol RNA was smarter amplified", "Experimental Factor: genotype:Gata2b +/ ", "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP144652", "Illumina NovaSeq 6000 paired end sequencing; Heterozygous Gata2b versus wildtype Gata2b zebrafish embryo HSPC CD41", "ENA FIRST PUBLIC:2023 02 17|ENA LAST UPDATE:2023 02 17", "Het1_cd41pflt1p48hpf_R1.fastq.gz Het1_cd41pflt1p48hpf_R2.fastq.gz", "fastq fastq", 36682350994.0, 181595797.0, "E MTAB 12577:Het1 cd41pflt1p48hpf R", "0:101 1:101", "A:10130106739;C:7969045717;G:8373740062;T:10209360741;N:97735", 101, 101, null, null, 10130106739, 7969045717, 8373740062, 10209360741, 97735, "ERX10296226", "ERS14601253", "ERA20429817", "Department of Hematology cancer institute ErasmusMC|European Nucleotide Archive", "Department of Hematology cancer institute ErasmusMC|European Nucleotide Archive", 2, 0.94302, 0.91104, 0.11873, 0.11597, 0.7554, 0.76579, 0.48813, 0.47764, 101, 101, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "full_length", "cdna_unspecified", "smarter", "bulk", "unknown", "unknown", null, "Netherlands", "2023-02-17", "Hatching", "Embryo", "Embryo Imprecise", "All anatomical structures"], [11723, "ERR11422840", "ERX10830011", "ERS15422295", "ERP147133", "PRJEB62042", "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "E-MTAB-12934", "Transcriptome Analysis", "RNA seq of zebrafish embryos with mutations in srpk3 sa1890 allele and/or ttn.1 sa5562 allele. Each of the 24 samples six samples for each of four genotypes represents RNA from a pool of three 5 dpf zebrafish embryos.", "ENA FIRST PUBLIC:2024 02 09|ENA LAST UPDATE:2024 02 09", null, "Protocols: Zebrafish embryos were collected for DNA and RNA extraction at 5 dpf in 6 pools of 3 embryos per genotype. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3 M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was then removed without xxx the beads. Whilst still on the magnet  the beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalog number M0303L. NEBNext Ultra II DNA Library Prep Kit for Illumina.", "srpk3 hom ttn1 het 1", "SAMEA113427169", "QMUL", "ENA FIRST PUBLIC:2024 02 09T16:00:07Z|ENA LAST UPDATE:2024 02 09T16:00:07Z|External Id:SAMEA113427169|INSDC center name:QMUL|INSDC first public:2024 02 09T16:00:07Z|INSDC last update:2024 02 09T16:00:07Z|INSDC status:public|Submitter Id:E MTAB 12934:srpk3 hom ttn1 het 1|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo|genotype:srpk3  / ; ttn.1 +/ |individual:pool of 3 embryos 1|organism part:embryo|replicate:1|sample name:E MTAB 12934:srpk3 hom ttn1 het 1|scientific name:Danio rerio|sex:not available|strain:T\u00fcpfel long fin", null, null, null, null, null, null, null, null, "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "E MTAB 12934:srpk3 hom ttn1 het 1 p", "srpk3 hom ttn1 het 1 p", "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "Zebrafish embryos were collected for DNA and RNA extraction at 5 dpf in 6 pools of 3 embryos per genotype. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3 M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was then removed without xxx the beads. Whilst still on the magnet  the beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalog number M0303L. NEBNext Ultra II DNA Library Prep Kit for Illumina.", "Experimental Factor: genotype:srpk3  / ; ttn.1 +/ ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP147133", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "ENA FIRST PUBLIC:2024 02 09|ENA LAST UPDATE:2024 02 09", "SLX-21419.C4.HV2TTDRXY.s_2.r_1.fq.gz SLX-21419.C4.HV2TTDRXY.s_2.r_2.fq.gz", "fastq fastq", 5201588100.0, 17338627.0, "E MTAB 12934:SLX 21419.C4.HV2TTDRXY.s 2.r ", "0:150 1:150", "A:1395812979;C:1209220590;G:1233107747;T:1363200419;N:246365", 150, 150, null, null, 1395812979, 1209220590, 1233107747, 1363200419, 246365, "ERX10830011", "ERS15422295", "ERA23329494", "European Bioinformatics Institute|European Nucleotide Archive", "European Bioinformatics Institute|European Nucleotide Archive", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "nebnext", "bulk", "unknown", "unknown", null, "United Kingdom", "2024-02-09", "Larval", "Larval", "Embryo Imprecise", "All anatomical structures"], [11724, "ERR11422856", "ERX10830027", "ERS15422311", "ERP147133", "PRJEB62042", "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "E-MTAB-12934", "Transcriptome Analysis", "RNA seq of zebrafish embryos with mutations in srpk3 sa1890 allele and/or ttn.1 sa5562 allele. Each of the 24 samples six samples for each of four genotypes represents RNA from a pool of three 5 dpf zebrafish embryos.", "ENA FIRST PUBLIC:2024 02 09|ENA LAST UPDATE:2024 02 09", null, "Protocols: Zebrafish embryos were collected for DNA and RNA extraction at 5 dpf in 6 pools of 3 embryos per genotype. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3 M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was then removed without xxx the beads. Whilst still on the magnet  the beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalog number M0303L. NEBNext Ultra II DNA Library Prep Kit for Illumina.", "srpk3 wt ttn1 het 8", "SAMEA113427185", "QMUL", "ENA FIRST PUBLIC:2024 02 09T16:00:07Z|ENA LAST UPDATE:2024 02 09T16:00:07Z|External Id:SAMEA113427185|INSDC center name:QMUL|INSDC first public:2024 02 09T16:00:07Z|INSDC last update:2024 02 09T16:00:07Z|INSDC status:public|Submitter Id:E MTAB 12934:srpk3 wt ttn1 het 8|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo|genotype:srpk3 +/+; ttn.1 +/ |individual:pool of 3 embryos 8|organism part:embryo|replicate:8|sample name:E MTAB 12934:srpk3 wt ttn1 het 8|scientific name:Danio rerio|sex:not available|strain:T\u00fcpfel long fin", null, null, null, null, null, null, null, null, "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "E MTAB 12934:srpk3 wt ttn1 het 8 p", "srpk3 wt ttn1 het 8 p", "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "Zebrafish embryos were collected for DNA and RNA extraction at 5 dpf in 6 pools of 3 embryos per genotype. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3 M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was then removed without xxx the beads. Whilst still on the magnet  the beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalog number M0303L. NEBNext Ultra II DNA Library Prep Kit for Illumina.", "Experimental Factor: genotype:srpk3 +/+; ttn.1 +/ ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP147133", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "ENA FIRST PUBLIC:2024 02 09|ENA LAST UPDATE:2024 02 09", "SLX-21419.C5.HV2TTDRXY.s_2.r_1.fq.gz SLX-21419.C5.HV2TTDRXY.s_2.r_2.fq.gz", "fastq fastq", 6243031800.0, 20810106.0, "E MTAB 12934:SLX 21419.C5.HV2TTDRXY.s 2.r ", "0:150 1:150", "A:1678428066;C:1448795147;G:1483028868;T:1632478253;N:301466", 150, 150, null, null, 1678428066, 1448795147, 1483028868, 1632478253, 301466, "ERX10830027", "ERS15422311", "ERA23329494", "European Bioinformatics Institute|European Nucleotide Archive", "European Bioinformatics Institute|European Nucleotide Archive", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "nebnext", "bulk", "unknown", "unknown", null, "United Kingdom", "2024-02-09", "Larval", "Larval", "Embryo Imprecise", "All anatomical structures"], [11725, "ERR11422863", "ERX10830034", "ERS15422318", "ERP147133", "PRJEB62042", "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "E-MTAB-12934", "Transcriptome Analysis", "RNA seq of zebrafish embryos with mutations in srpk3 sa1890 allele and/or ttn.1 sa5562 allele. Each of the 24 samples six samples for each of four genotypes represents RNA from a pool of three 5 dpf zebrafish embryos.", "ENA FIRST PUBLIC:2024 02 09|ENA LAST UPDATE:2024 02 09", null, "Protocols: Zebrafish embryos were collected for DNA and RNA extraction at 5 dpf in 6 pools of 3 embryos per genotype. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3 M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was then removed without xxx the beads. Whilst still on the magnet  the beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalog number M0303L. NEBNext Ultra II DNA Library Prep Kit for Illumina.", "srpk3 wt ttn1 wt 7", "SAMEA113427192", "QMUL", "ENA FIRST PUBLIC:2024 02 09T16:00:07Z|ENA LAST UPDATE:2024 02 09T16:00:07Z|External Id:SAMEA113427192|INSDC center name:QMUL|INSDC first public:2024 02 09T16:00:07Z|INSDC last update:2024 02 09T16:00:07Z|INSDC status:public|Submitter Id:E MTAB 12934:srpk3 wt ttn1 wt 7|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo|genotype:srpk3 +/+; ttn.1 +/+|individual:pool of 3 embryos 7|organism part:embryo|replicate:7|sample name:E MTAB 12934:srpk3 wt ttn1 wt 7|scientific name:Danio rerio|sex:not available|strain:T\u00fcpfel long fin", null, null, null, null, null, null, null, null, "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "E MTAB 12934:srpk3 wt ttn1 wt 7 p", "srpk3 wt ttn1 wt 7 p", "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "Zebrafish embryos were collected for DNA and RNA extraction at 5 dpf in 6 pools of 3 embryos per genotype. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3 M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was then removed without xxx the beads. Whilst still on the magnet  the beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalog number M0303L. NEBNext Ultra II DNA Library Prep Kit for Illumina.", "Experimental Factor: genotype:srpk3 +/+; ttn.1 +/+", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP147133", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "ENA FIRST PUBLIC:2024 02 09|ENA LAST UPDATE:2024 02 09", "SLX-21419.G3.HV2TTDRXY.s_2.r_1.fq.gz SLX-21419.G3.HV2TTDRXY.s_2.r_2.fq.gz", "fastq fastq", 7924538100.0, 26415127.0, "E MTAB 12934:SLX 21419.G3.HV2TTDRXY.s 2.r ", "0:150 1:150", "A:2128544326;C:1844570027;G:1875605207;T:2075443615;N:374925", 150, 150, null, null, 2128544326, 1844570027, 1875605207, 2075443615, 374925, "ERX10830034", "ERS15422318", "ERA23329494", "European Bioinformatics Institute|European Nucleotide Archive", "European Bioinformatics Institute|European Nucleotide Archive", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "nebnext", "bulk", "unknown", "unknown", null, "United Kingdom", "2024-02-09", "Larval", "Larval", "Embryo Imprecise", "All anatomical structures"], [11726, "ERR11422862", "ERX10830033", "ERS15422317", "ERP147133", "PRJEB62042", "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "E-MTAB-12934", "Transcriptome Analysis", "RNA seq of zebrafish embryos with mutations in srpk3 sa1890 allele and/or ttn.1 sa5562 allele. Each of the 24 samples six samples for each of four genotypes represents RNA from a pool of three 5 dpf zebrafish embryos.", "ENA FIRST PUBLIC:2024 02 09|ENA LAST UPDATE:2024 02 09", null, "Protocols: Zebrafish embryos were collected for DNA and RNA extraction at 5 dpf in 6 pools of 3 embryos per genotype. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3 M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was then removed without xxx the beads. Whilst still on the magnet  the beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalog number M0303L. NEBNext Ultra II DNA Library Prep Kit for Illumina.", "srpk3 wt ttn1 wt 6", "SAMEA113427191", "QMUL", "ENA FIRST PUBLIC:2024 02 09T16:00:07Z|ENA LAST UPDATE:2024 02 09T16:00:07Z|External Id:SAMEA113427191|INSDC center name:QMUL|INSDC first public:2024 02 09T16:00:07Z|INSDC last update:2024 02 09T16:00:07Z|INSDC status:public|Submitter Id:E MTAB 12934:srpk3 wt ttn1 wt 6|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo|genotype:srpk3 +/+; ttn.1 +/+|individual:pool of 3 embryos 6|organism part:embryo|replicate:6|sample name:E MTAB 12934:srpk3 wt ttn1 wt 6|scientific name:Danio rerio|sex:not available|strain:T\u00fcpfel long fin", null, null, null, null, null, null, null, null, "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "E MTAB 12934:srpk3 wt ttn1 wt 6 p", "srpk3 wt ttn1 wt 6 p", "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "Zebrafish embryos were collected for DNA and RNA extraction at 5 dpf in 6 pools of 3 embryos per genotype. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3 M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was then removed without xxx the beads. Whilst still on the magnet  the beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalog number M0303L. NEBNext Ultra II DNA Library Prep Kit for Illumina.", "Experimental Factor: genotype:srpk3 +/+; ttn.1 +/+", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP147133", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "ENA FIRST PUBLIC:2024 02 09|ENA LAST UPDATE:2024 02 09", "SLX-21419.D5.HV2TTDRXY.s_2.r_1.fq.gz SLX-21419.D5.HV2TTDRXY.s_2.r_2.fq.gz", "fastq fastq", 8652848700.0, 28842829.0, "E MTAB 12934:SLX 21419.D5.HV2TTDRXY.s 2.r ", "0:150 1:150", "A:2323784475;C:2011287942;G:2055678520;T:2261686116;N:411647", 150, 150, null, null, 2323784475, 2011287942, 2055678520, 2261686116, 411647, "ERX10830033", "ERS15422317", "ERA23329494", "European Bioinformatics Institute|European Nucleotide Archive", "European Bioinformatics Institute|European Nucleotide Archive", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "nebnext", "bulk", "unknown", "unknown", null, "United Kingdom", "2024-02-09", "Larval", "Larval", "Embryo Imprecise", "All anatomical structures"], [11727, "ERR11422852", "ERX10830023", "ERS15422307", "ERP147133", "PRJEB62042", "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "E-MTAB-12934", "Transcriptome Analysis", "RNA seq of zebrafish embryos with mutations in srpk3 sa1890 allele and/or ttn.1 sa5562 allele. Each of the 24 samples six samples for each of four genotypes represents RNA from a pool of three 5 dpf zebrafish embryos.", "ENA FIRST PUBLIC:2024 02 09|ENA LAST UPDATE:2024 02 09", null, "Protocols: Zebrafish embryos were collected for DNA and RNA extraction at 5 dpf in 6 pools of 3 embryos per genotype. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3 M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was then removed without xxx the beads. Whilst still on the magnet  the beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalog number M0303L. NEBNext Ultra II DNA Library Prep Kit for Illumina.", "srpk3 wt ttn1 het 1", "SAMEA113427181", "QMUL", "ENA FIRST PUBLIC:2024 02 09T16:00:07Z|ENA LAST UPDATE:2024 02 09T16:00:07Z|External Id:SAMEA113427181|INSDC center name:QMUL|INSDC first public:2024 02 09T16:00:07Z|INSDC last update:2024 02 09T16:00:07Z|INSDC status:public|Submitter Id:E MTAB 12934:srpk3 wt ttn1 het 1|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo|genotype:srpk3 +/+; ttn.1 +/ |individual:pool of 3 embryos 1|organism part:embryo|replicate:1|sample name:E MTAB 12934:srpk3 wt ttn1 het 1|scientific name:Danio rerio|sex:not available|strain:T\u00fcpfel long fin", null, null, null, null, null, null, null, null, "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "E MTAB 12934:srpk3 wt ttn1 het 1 p", "srpk3 wt ttn1 het 1 p", "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "Zebrafish embryos were collected for DNA and RNA extraction at 5 dpf in 6 pools of 3 embryos per genotype. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3 M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was then removed without xxx the beads. Whilst still on the magnet  the beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalog number M0303L. NEBNext Ultra II DNA Library Prep Kit for Illumina.", "Experimental Factor: genotype:srpk3 +/+; ttn.1 +/ ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP147133", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "ENA FIRST PUBLIC:2024 02 09|ENA LAST UPDATE:2024 02 09", "SLX-21419.E3.HV2TTDRXY.s_2.r_1.fq.gz SLX-21419.E3.HV2TTDRXY.s_2.r_2.fq.gz", "fastq fastq", 5595162300.0, 18650541.0, "E MTAB 12934:SLX 21419.E3.HV2TTDRXY.s 2.r ", "0:150 1:150", "A:1500582422;C:1301393746;G:1326420086;T:1466508237;N:257809", 150, 150, null, null, 1500582422, 1301393746, 1326420086, 1466508237, 257809, "ERX10830023", "ERS15422307", "ERA23329494", "European Bioinformatics Institute|European Nucleotide Archive", "European Bioinformatics Institute|European Nucleotide Archive", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "nebnext", "bulk", "unknown", "unknown", null, "United Kingdom", "2024-02-09", "Larval", "Larval", "Embryo Imprecise", "All anatomical structures"], [11728, "ERR11422841", "ERX10830012", "ERS15422296", "ERP147133", "PRJEB62042", "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "E-MTAB-12934", "Transcriptome Analysis", "RNA seq of zebrafish embryos with mutations in srpk3 sa1890 allele and/or ttn.1 sa5562 allele. Each of the 24 samples six samples for each of four genotypes represents RNA from a pool of three 5 dpf zebrafish embryos.", "ENA FIRST PUBLIC:2024 02 09|ENA LAST UPDATE:2024 02 09", null, "Protocols: Zebrafish embryos were collected for DNA and RNA extraction at 5 dpf in 6 pools of 3 embryos per genotype. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3 M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was then removed without xxx the beads. Whilst still on the magnet  the beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalog number M0303L. NEBNext Ultra II DNA Library Prep Kit for Illumina.", "srpk3 hom ttn1 het 10", "SAMEA113427170", "QMUL", "ENA FIRST PUBLIC:2024 02 09T16:00:07Z|ENA LAST UPDATE:2024 02 09T16:00:07Z|External Id:SAMEA113427170|INSDC center name:QMUL|INSDC first public:2024 02 09T16:00:07Z|INSDC last update:2024 02 09T16:00:07Z|INSDC status:public|Submitter Id:E MTAB 12934:srpk3 hom ttn1 het 10|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo|genotype:srpk3  / ; ttn.1 +/ |individual:pool of 3 embryos 10|organism part:embryo|replicate:10|sample name:E MTAB 12934:srpk3 hom ttn1 het 10|scientific name:Danio rerio|sex:not available|strain:T\u00fcpfel long fin", null, null, null, null, null, null, null, null, "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "E MTAB 12934:srpk3 hom ttn1 het 10 p", "srpk3 hom ttn1 het 10 p", "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "Zebrafish embryos were collected for DNA and RNA extraction at 5 dpf in 6 pools of 3 embryos per genotype. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3 M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was then removed without xxx the beads. Whilst still on the magnet  the beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalog number M0303L. NEBNext Ultra II DNA Library Prep Kit for Illumina.", "Experimental Factor: genotype:srpk3  / ; ttn.1 +/ ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP147133", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "ENA FIRST PUBLIC:2024 02 09|ENA LAST UPDATE:2024 02 09", "SLX-21419.F2.HV2TTDRXY.s_2.r_1.fq.gz SLX-21419.F2.HV2TTDRXY.s_2.r_2.fq.gz", "fastq fastq", 6163159800.0, 20543866.0, "E MTAB 12934:SLX 21419.F2.HV2TTDRXY.s 2.r ", "0:150 1:150", "A:1651051246;C:1435397149;G:1465377920;T:1611039390;N:294095", 150, 150, null, null, 1651051246, 1435397149, 1465377920, 1611039390, 294095, "ERX10830012", "ERS15422296", "ERA23329494", "European Bioinformatics Institute|European Nucleotide Archive", "European Bioinformatics Institute|European Nucleotide Archive", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "nebnext", "bulk", "unknown", "unknown", null, "United Kingdom", "2024-02-09", "Larval", "Larval", "Embryo Imprecise", "All anatomical structures"], [11729, "ERR11422858", "ERX10830029", "ERS15422313", "ERP147133", "PRJEB62042", "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "E-MTAB-12934", "Transcriptome Analysis", "RNA seq of zebrafish embryos with mutations in srpk3 sa1890 allele and/or ttn.1 sa5562 allele. Each of the 24 samples six samples for each of four genotypes represents RNA from a pool of three 5 dpf zebrafish embryos.", "ENA FIRST PUBLIC:2024 02 09|ENA LAST UPDATE:2024 02 09", null, "Protocols: Zebrafish embryos were collected for DNA and RNA extraction at 5 dpf in 6 pools of 3 embryos per genotype. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3 M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was then removed without xxx the beads. Whilst still on the magnet  the beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalog number M0303L. NEBNext Ultra II DNA Library Prep Kit for Illumina.", "srpk3 wt ttn1 wt 2", "SAMEA113427187", "QMUL", "ENA FIRST PUBLIC:2024 02 09T16:00:07Z|ENA LAST UPDATE:2024 02 09T16:00:07Z|External Id:SAMEA113427187|INSDC center name:QMUL|INSDC first public:2024 02 09T16:00:07Z|INSDC last update:2024 02 09T16:00:07Z|INSDC status:public|Submitter Id:E MTAB 12934:srpk3 wt ttn1 wt 2|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo|genotype:srpk3 +/+; ttn.1 +/+|individual:pool of 3 embryos 2|organism part:embryo|replicate:2|sample name:E MTAB 12934:srpk3 wt ttn1 wt 2|scientific name:Danio rerio|sex:not available|strain:T\u00fcpfel long fin", null, null, null, null, null, null, null, null, "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "E MTAB 12934:srpk3 wt ttn1 wt 2 p", "srpk3 wt ttn1 wt 2 p", "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "Zebrafish embryos were collected for DNA and RNA extraction at 5 dpf in 6 pools of 3 embryos per genotype. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3 M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was then removed without xxx the beads. Whilst still on the magnet  the beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalog number M0303L. NEBNext Ultra II DNA Library Prep Kit for Illumina.", "Experimental Factor: genotype:srpk3 +/+; ttn.1 +/+", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP147133", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "ENA FIRST PUBLIC:2024 02 09|ENA LAST UPDATE:2024 02 09", "SLX-21419.E4.HV2TTDRXY.s_2.r_1.fq.gz SLX-21419.E4.HV2TTDRXY.s_2.r_2.fq.gz", "fastq fastq", 4948755000.0, 16495850.0, "E MTAB 12934:SLX 21419.E4.HV2TTDRXY.s 2.r ", "0:150 1:150", "A:1328315519;C:1150664187;G:1172962823;T:1296576134;N:236337", 150, 150, null, null, 1328315519, 1150664187, 1172962823, 1296576134, 236337, "ERX10830029", "ERS15422313", "ERA23329494", "European Bioinformatics Institute|European Nucleotide Archive", "European Bioinformatics Institute|European Nucleotide Archive", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "nebnext", "bulk", "unknown", "unknown", null, "United Kingdom", "2024-02-09", "Larval", "Larval", "Embryo Imprecise", "All anatomical structures"], [11730, "ERR11422853", "ERX10830024", "ERS15422308", "ERP147133", "PRJEB62042", "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "E-MTAB-12934", "Transcriptome Analysis", "RNA seq of zebrafish embryos with mutations in srpk3 sa1890 allele and/or ttn.1 sa5562 allele. Each of the 24 samples six samples for each of four genotypes represents RNA from a pool of three 5 dpf zebrafish embryos.", "ENA FIRST PUBLIC:2024 02 09|ENA LAST UPDATE:2024 02 09", null, "Protocols: Zebrafish embryos were collected for DNA and RNA extraction at 5 dpf in 6 pools of 3 embryos per genotype. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3 M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was then removed without xxx the beads. Whilst still on the magnet  the beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalog number M0303L. NEBNext Ultra II DNA Library Prep Kit for Illumina.", "srpk3 wt ttn1 het 2", "SAMEA113427182", "QMUL", "ENA FIRST PUBLIC:2024 02 09T16:00:07Z|ENA LAST UPDATE:2024 02 09T16:00:07Z|External Id:SAMEA113427182|INSDC center name:QMUL|INSDC first public:2024 02 09T16:00:07Z|INSDC last update:2024 02 09T16:00:07Z|INSDC status:public|Submitter Id:E MTAB 12934:srpk3 wt ttn1 het 2|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo|genotype:srpk3 +/+; ttn.1 +/ |individual:pool of 3 embryos 2|organism part:embryo|replicate:2|sample name:E MTAB 12934:srpk3 wt ttn1 het 2|scientific name:Danio rerio|sex:not available|strain:T\u00fcpfel long fin", null, null, null, null, null, null, null, null, "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "E MTAB 12934:srpk3 wt ttn1 het 2 p", "srpk3 wt ttn1 het 2 p", "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "Zebrafish embryos were collected for DNA and RNA extraction at 5 dpf in 6 pools of 3 embryos per genotype. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3 M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was then removed without xxx the beads. Whilst still on the magnet  the beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalog number M0303L. NEBNext Ultra II DNA Library Prep Kit for Illumina.", "Experimental Factor: genotype:srpk3 +/+; ttn.1 +/ ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP147133", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "ENA FIRST PUBLIC:2024 02 09|ENA LAST UPDATE:2024 02 09", "SLX-21419.A5.HV2TTDRXY.s_2.r_1.fq.gz SLX-21419.A5.HV2TTDRXY.s_2.r_2.fq.gz", "fastq fastq", 5172515100.0, 17241717.0, "E MTAB 12934:SLX 21419.A5.HV2TTDRXY.s 2.r ", "0:150 1:150", "A:1390660856;C:1200693628;G:1226597252;T:1354312680;N:250684", 150, 150, null, null, 1390660856, 1200693628, 1226597252, 1354312680, 250684, "ERX10830024", "ERS15422308", "ERA23329494", "European Bioinformatics Institute|European Nucleotide Archive", "European Bioinformatics Institute|European Nucleotide Archive", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "nebnext", "bulk", "unknown", "unknown", null, "United Kingdom", "2024-02-09", "Larval", "Larval", "Embryo Imprecise", "All anatomical structures"], [11731, "ERR11422842", "ERX10830013", "ERS15422297", "ERP147133", "PRJEB62042", "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "E-MTAB-12934", "Transcriptome Analysis", "RNA seq of zebrafish embryos with mutations in srpk3 sa1890 allele and/or ttn.1 sa5562 allele. Each of the 24 samples six samples for each of four genotypes represents RNA from a pool of three 5 dpf zebrafish embryos.", "ENA FIRST PUBLIC:2024 02 09|ENA LAST UPDATE:2024 02 09", null, "Protocols: Zebrafish embryos were collected for DNA and RNA extraction at 5 dpf in 6 pools of 3 embryos per genotype. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3 M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was then removed without xxx the beads. Whilst still on the magnet  the beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalog number M0303L. NEBNext Ultra II DNA Library Prep Kit for Illumina.", "srpk3 hom ttn1 het 2", "SAMEA113427171", "QMUL", "ENA FIRST PUBLIC:2024 02 09T16:00:07Z|ENA LAST UPDATE:2024 02 09T16:00:07Z|External Id:SAMEA113427171|INSDC center name:QMUL|INSDC first public:2024 02 09T16:00:07Z|INSDC last update:2024 02 09T16:00:07Z|INSDC status:public|Submitter Id:E MTAB 12934:srpk3 hom ttn1 het 2|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo|genotype:srpk3  / ; ttn.1 +/ |individual:pool of 3 embryos 2|organism part:embryo|replicate:2|sample name:E MTAB 12934:srpk3 hom ttn1 het 2|scientific name:Danio rerio|sex:not available|strain:T\u00fcpfel long fin", null, null, null, null, null, null, null, null, "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "E MTAB 12934:srpk3 hom ttn1 het 2 p", "srpk3 hom ttn1 het 2 p", "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "Zebrafish embryos were collected for DNA and RNA extraction at 5 dpf in 6 pools of 3 embryos per genotype. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3 M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was then removed without xxx the beads. Whilst still on the magnet  the beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalog number M0303L. NEBNext Ultra II DNA Library Prep Kit for Illumina.", "Experimental Factor: genotype:srpk3  / ; ttn.1 +/ ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP147133", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "ENA FIRST PUBLIC:2024 02 09|ENA LAST UPDATE:2024 02 09", "SLX-21419.B3.HV2TTDRXY.s_2.r_1.fq.gz SLX-21419.B3.HV2TTDRXY.s_2.r_2.fq.gz", "fastq fastq", 7596727500.0, 25322425.0, "E MTAB 12934:SLX 21419.B3.HV2TTDRXY.s 2.r ", "0:150 1:150", "A:2040744877;C:1765753859;G:1800431343;T:1989434039;N:363382", 150, 150, null, null, 2040744877, 1765753859, 1800431343, 1989434039, 363382, "ERX10830013", "ERS15422297", "ERA23329494", "European Bioinformatics Institute|European Nucleotide Archive", "European Bioinformatics Institute|European Nucleotide Archive", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "nebnext", "bulk", "unknown", "unknown", null, "United Kingdom", "2024-02-09", "Larval", "Larval", "Embryo Imprecise", "All anatomical structures"], [11732, "ERR11422854", "ERX10830025", "ERS15422309", "ERP147133", "PRJEB62042", "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "E-MTAB-12934", "Transcriptome Analysis", "RNA seq of zebrafish embryos with mutations in srpk3 sa1890 allele and/or ttn.1 sa5562 allele. Each of the 24 samples six samples for each of four genotypes represents RNA from a pool of three 5 dpf zebrafish embryos.", "ENA FIRST PUBLIC:2024 02 09|ENA LAST UPDATE:2024 02 09", null, "Protocols: Zebrafish embryos were collected for DNA and RNA extraction at 5 dpf in 6 pools of 3 embryos per genotype. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3 M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was then removed without xxx the beads. Whilst still on the magnet  the beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalog number M0303L. NEBNext Ultra II DNA Library Prep Kit for Illumina.", "srpk3 wt ttn1 het 3", "SAMEA113427183", "QMUL", "ENA FIRST PUBLIC:2024 02 09T16:00:07Z|ENA LAST UPDATE:2024 02 09T16:00:07Z|External Id:SAMEA113427183|INSDC center name:QMUL|INSDC first public:2024 02 09T16:00:07Z|INSDC last update:2024 02 09T16:00:07Z|INSDC status:public|Submitter Id:E MTAB 12934:srpk3 wt ttn1 het 3|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo|genotype:srpk3 +/+; ttn.1 +/ |individual:pool of 3 embryos 3|organism part:embryo|replicate:3|sample name:E MTAB 12934:srpk3 wt ttn1 het 3|scientific name:Danio rerio|sex:not available|strain:T\u00fcpfel long fin", null, null, null, null, null, null, null, null, "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "E MTAB 12934:srpk3 wt ttn1 het 3 p", "srpk3 wt ttn1 het 3 p", "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "Zebrafish embryos were collected for DNA and RNA extraction at 5 dpf in 6 pools of 3 embryos per genotype. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3 M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was then removed without xxx the beads. Whilst still on the magnet  the beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalog number M0303L. NEBNext Ultra II DNA Library Prep Kit for Illumina.", "Experimental Factor: genotype:srpk3 +/+; ttn.1 +/ ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP147133", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "ENA FIRST PUBLIC:2024 02 09|ENA LAST UPDATE:2024 02 09", "SLX-21419.B4.HV2TTDRXY.s_2.r_1.fq.gz SLX-21419.B4.HV2TTDRXY.s_2.r_2.fq.gz", "fastq fastq", 4147455600.0, 13824852.0, "E MTAB 12934:SLX 21419.B4.HV2TTDRXY.s 2.r ", "0:150 1:150", "A:1111933168;C:965101703;G:986324158;T:1083898570;N:198001", 150, 150, null, null, 1111933168, 965101703, 986324158, 1083898570, 198001, "ERX10830025", "ERS15422309", "ERA23329494", "European Bioinformatics Institute|European Nucleotide Archive", "European Bioinformatics Institute|European Nucleotide Archive", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "nebnext", "bulk", "unknown", "unknown", null, "United Kingdom", "2024-02-09", "Larval", "Larval", "Embryo Imprecise", "All anatomical structures"], [11733, "ERR11422855", "ERX10830026", "ERS15422310", "ERP147133", "PRJEB62042", "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "E-MTAB-12934", "Transcriptome Analysis", "RNA seq of zebrafish embryos with mutations in srpk3 sa1890 allele and/or ttn.1 sa5562 allele. Each of the 24 samples six samples for each of four genotypes represents RNA from a pool of three 5 dpf zebrafish embryos.", "ENA FIRST PUBLIC:2024 02 09|ENA LAST UPDATE:2024 02 09", null, "Protocols: Zebrafish embryos were collected for DNA and RNA extraction at 5 dpf in 6 pools of 3 embryos per genotype. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3 M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was then removed without xxx the beads. Whilst still on the magnet  the beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalog number M0303L. NEBNext Ultra II DNA Library Prep Kit for Illumina.", "srpk3 wt ttn1 het 7", "SAMEA113427184", "QMUL", "ENA FIRST PUBLIC:2024 02 09T16:00:07Z|ENA LAST UPDATE:2024 02 09T16:00:07Z|External Id:SAMEA113427184|INSDC center name:QMUL|INSDC first public:2024 02 09T16:00:07Z|INSDC last update:2024 02 09T16:00:07Z|INSDC status:public|Submitter Id:E MTAB 12934:srpk3 wt ttn1 het 7|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo|genotype:srpk3 +/+; ttn.1 +/ |individual:pool of 3 embryos 7|organism part:embryo|replicate:7|sample name:E MTAB 12934:srpk3 wt ttn1 het 7|scientific name:Danio rerio|sex:not available|strain:T\u00fcpfel long fin", null, null, null, null, null, null, null, null, "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "E MTAB 12934:srpk3 wt ttn1 het 7 p", "srpk3 wt ttn1 het 7 p", "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "Zebrafish embryos were collected for DNA and RNA extraction at 5 dpf in 6 pools of 3 embryos per genotype. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3 M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was then removed without xxx the beads. Whilst still on the magnet  the beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalog number M0303L. NEBNext Ultra II DNA Library Prep Kit for Illumina.", "Experimental Factor: genotype:srpk3 +/+; ttn.1 +/ ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP147133", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "ENA FIRST PUBLIC:2024 02 09|ENA LAST UPDATE:2024 02 09", "SLX-21419.C3.HV2TTDRXY.s_2.r_1.fq.gz SLX-21419.C3.HV2TTDRXY.s_2.r_2.fq.gz", "fastq fastq", 7815347400.0, 26051158.0, "E MTAB 12934:SLX 21419.C3.HV2TTDRXY.s 2.r ", "0:150 1:150", "A:2105543787;C:1810060936;G:1848658748;T:2050709339;N:374590", 150, 150, null, null, 2105543787, 1810060936, 1848658748, 2050709339, 374590, "ERX10830026", "ERS15422310", "ERA23329494", "European Bioinformatics Institute|European Nucleotide Archive", "European Bioinformatics Institute|European Nucleotide Archive", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "nebnext", "bulk", "unknown", "unknown", null, "United Kingdom", "2024-02-09", "Larval", "Larval", "Embryo Imprecise", "All anatomical structures"], [11734, "ERR11422847", "ERX10830018", "ERS15422302", "ERP147133", "PRJEB62042", "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "E-MTAB-12934", "Transcriptome Analysis", "RNA seq of zebrafish embryos with mutations in srpk3 sa1890 allele and/or ttn.1 sa5562 allele. Each of the 24 samples six samples for each of four genotypes represents RNA from a pool of three 5 dpf zebrafish embryos.", "ENA FIRST PUBLIC:2024 02 09|ENA LAST UPDATE:2024 02 09", null, "Protocols: Zebrafish embryos were collected for DNA and RNA extraction at 5 dpf in 6 pools of 3 embryos per genotype. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3 M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was then removed without xxx the beads. Whilst still on the magnet  the beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalog number M0303L. NEBNext Ultra II DNA Library Prep Kit for Illumina.", "srpk3 hom ttn1 wt 4", "SAMEA113427176", "QMUL", "ENA FIRST PUBLIC:2024 02 09T16:00:07Z|ENA LAST UPDATE:2024 02 09T16:00:07Z|External Id:SAMEA113427176|INSDC center name:QMUL|INSDC first public:2024 02 09T16:00:07Z|INSDC last update:2024 02 09T16:00:07Z|INSDC status:public|Submitter Id:E MTAB 12934:srpk3 hom ttn1 wt 4|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo|genotype:srpk3  / ; ttn.1 +/+|individual:pool of 3 embryos 4|organism part:embryo|replicate:4|sample name:E MTAB 12934:srpk3 hom ttn1 wt 4|scientific name:Danio rerio|sex:not available|strain:T\u00fcpfel long fin", null, null, null, null, null, null, null, null, "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "E MTAB 12934:srpk3 hom ttn1 wt 4 p", "srpk3 hom ttn1 wt 4 p", "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "Zebrafish embryos were collected for DNA and RNA extraction at 5 dpf in 6 pools of 3 embryos per genotype. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3 M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was then removed without xxx the beads. Whilst still on the magnet  the beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalog number M0303L. NEBNext Ultra II DNA Library Prep Kit for Illumina.", "Experimental Factor: genotype:srpk3  / ; ttn.1 +/+", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP147133", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "ENA FIRST PUBLIC:2024 02 09|ENA LAST UPDATE:2024 02 09", "SLX-21419.F4.HV2TTDRXY.s_2.r_1.fq.gz SLX-21419.F4.HV2TTDRXY.s_2.r_2.fq.gz", "fastq fastq", 5552048400.0, 18506828.0, "E MTAB 12934:SLX 21419.F4.HV2TTDRXY.s 2.r ", "0:150 1:150", "A:1494691750;C:1287655327;G:1313223405;T:1456215647;N:262271", 150, 150, null, null, 1494691750, 1287655327, 1313223405, 1456215647, 262271, "ERX10830018", "ERS15422302", "ERA23329494", "European Bioinformatics Institute|European Nucleotide Archive", "European Bioinformatics Institute|European Nucleotide Archive", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "nebnext", "bulk", "unknown", "unknown", null, "United Kingdom", "2024-02-09", "Larval", "Larval", "Embryo Imprecise", "All anatomical structures"], [11735, "ERR11422843", "ERX10830014", "ERS15422298", "ERP147133", "PRJEB62042", "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "E-MTAB-12934", "Transcriptome Analysis", "RNA seq of zebrafish embryos with mutations in srpk3 sa1890 allele and/or ttn.1 sa5562 allele. Each of the 24 samples six samples for each of four genotypes represents RNA from a pool of three 5 dpf zebrafish embryos.", "ENA FIRST PUBLIC:2024 02 09|ENA LAST UPDATE:2024 02 09", null, "Protocols: Zebrafish embryos were collected for DNA and RNA extraction at 5 dpf in 6 pools of 3 embryos per genotype. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3 M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was then removed without xxx the beads. Whilst still on the magnet  the beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalog number M0303L. NEBNext Ultra II DNA Library Prep Kit for Illumina.", "srpk3 hom ttn1 het 7", "SAMEA113427172", "QMUL", "ENA FIRST PUBLIC:2024 02 09T16:00:07Z|ENA LAST UPDATE:2024 02 09T16:00:07Z|External Id:SAMEA113427172|INSDC center name:QMUL|INSDC first public:2024 02 09T16:00:07Z|INSDC last update:2024 02 09T16:00:07Z|INSDC status:public|Submitter Id:E MTAB 12934:srpk3 hom ttn1 het 7|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo|genotype:srpk3  / ; ttn.1 +/ |individual:pool of 3 embryos 7|organism part:embryo|replicate:7|sample name:E MTAB 12934:srpk3 hom ttn1 het 7|scientific name:Danio rerio|sex:not available|strain:T\u00fcpfel long fin", null, null, null, null, null, null, null, null, "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "E MTAB 12934:srpk3 hom ttn1 het 7 p", "srpk3 hom ttn1 het 7 p", "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "Zebrafish embryos were collected for DNA and RNA extraction at 5 dpf in 6 pools of 3 embryos per genotype. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3 M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was then removed without xxx the beads. Whilst still on the magnet  the beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalog number M0303L. NEBNext Ultra II DNA Library Prep Kit for Illumina.", "Experimental Factor: genotype:srpk3  / ; ttn.1 +/ ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP147133", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "ENA FIRST PUBLIC:2024 02 09|ENA LAST UPDATE:2024 02 09", "SLX-21419.G2.HV2TTDRXY.s_2.r_1.fq.gz SLX-21419.G2.HV2TTDRXY.s_2.r_2.fq.gz", "fastq fastq", 6130593900.0, 20435313.0, "E MTAB 12934:SLX 21419.G2.HV2TTDRXY.s 2.r ", "0:150 1:150", "A:1646268883;C:1426071699;G:1453474221;T:1604484627;N:294470", 150, 150, null, null, 1646268883, 1426071699, 1453474221, 1604484627, 294470, "ERX10830014", "ERS15422298", "ERA23329494", "European Bioinformatics Institute|European Nucleotide Archive", "European Bioinformatics Institute|European Nucleotide Archive", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "nebnext", "bulk", "unknown", "unknown", null, "United Kingdom", "2024-02-09", "Larval", "Larval", "Embryo Imprecise", "All anatomical structures"], [11736, "ERR11422857", "ERX10830028", "ERS15422312", "ERP147133", "PRJEB62042", "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "E-MTAB-12934", "Transcriptome Analysis", "RNA seq of zebrafish embryos with mutations in srpk3 sa1890 allele and/or ttn.1 sa5562 allele. Each of the 24 samples six samples for each of four genotypes represents RNA from a pool of three 5 dpf zebrafish embryos.", "ENA FIRST PUBLIC:2024 02 09|ENA LAST UPDATE:2024 02 09", null, "Protocols: Zebrafish embryos were collected for DNA and RNA extraction at 5 dpf in 6 pools of 3 embryos per genotype. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3 M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was then removed without xxx the beads. Whilst still on the magnet  the beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalog number M0303L. NEBNext Ultra II DNA Library Prep Kit for Illumina.", "srpk3 wt ttn1 het 9", "SAMEA113427186", "QMUL", "ENA FIRST PUBLIC:2024 02 09T16:00:07Z|ENA LAST UPDATE:2024 02 09T16:00:07Z|External Id:SAMEA113427186|INSDC center name:QMUL|INSDC first public:2024 02 09T16:00:07Z|INSDC last update:2024 02 09T16:00:07Z|INSDC status:public|Submitter Id:E MTAB 12934:srpk3 wt ttn1 het 9|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo|genotype:srpk3 +/+; ttn.1 +/ |individual:pool of 3 embryos 9|organism part:embryo|replicate:9|sample name:E MTAB 12934:srpk3 wt ttn1 het 9|scientific name:Danio rerio|sex:not available|strain:T\u00fcpfel long fin", null, null, null, null, null, null, null, null, "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "E MTAB 12934:srpk3 wt ttn1 het 9 p", "srpk3 wt ttn1 het 9 p", "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "Zebrafish embryos were collected for DNA and RNA extraction at 5 dpf in 6 pools of 3 embryos per genotype. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3 M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was then removed without xxx the beads. Whilst still on the magnet  the beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalog number M0303L. NEBNext Ultra II DNA Library Prep Kit for Illumina.", "Experimental Factor: genotype:srpk3 +/+; ttn.1 +/ ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP147133", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "ENA FIRST PUBLIC:2024 02 09|ENA LAST UPDATE:2024 02 09", "SLX-21419.A3.HV2TTDRXY.s_2.r_1.fq.gz SLX-21419.A3.HV2TTDRXY.s_2.r_2.fq.gz", "fastq fastq", 7955802600.0, 26519342.0, "E MTAB 12934:SLX 21419.A3.HV2TTDRXY.s 2.r ", "0:150 1:150", "A:2139048525;C:1844468923;G:1881094685;T:2090808427;N:382040", 150, 150, null, null, 2139048525, 1844468923, 1881094685, 2090808427, 382040, "ERX10830028", "ERS15422312", "ERA23329494", "European Bioinformatics Institute|European Nucleotide Archive", "European Bioinformatics Institute|European Nucleotide Archive", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "nebnext", "bulk", "unknown", "unknown", null, "United Kingdom", "2024-02-09", "Larval", "Larval", "Embryo Imprecise", "All anatomical structures"], [11737, "ERR11422844", "ERX10830015", "ERS15422299", "ERP147133", "PRJEB62042", "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "E-MTAB-12934", "Transcriptome Analysis", "RNA seq of zebrafish embryos with mutations in srpk3 sa1890 allele and/or ttn.1 sa5562 allele. Each of the 24 samples six samples for each of four genotypes represents RNA from a pool of three 5 dpf zebrafish embryos.", "ENA FIRST PUBLIC:2024 02 09|ENA LAST UPDATE:2024 02 09", null, "Protocols: Zebrafish embryos were collected for DNA and RNA extraction at 5 dpf in 6 pools of 3 embryos per genotype. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3 M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was then removed without xxx the beads. Whilst still on the magnet  the beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalog number M0303L. NEBNext Ultra II DNA Library Prep Kit for Illumina.", "srpk3 hom ttn1 het 8", "SAMEA113427173", "QMUL", "ENA FIRST PUBLIC:2024 02 09T16:00:07Z|ENA LAST UPDATE:2024 02 09T16:00:07Z|External Id:SAMEA113427173|INSDC center name:QMUL|INSDC first public:2024 02 09T16:00:07Z|INSDC last update:2024 02 09T16:00:07Z|INSDC status:public|Submitter Id:E MTAB 12934:srpk3 hom ttn1 het 8|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo|genotype:srpk3  / ; ttn.1 +/ |individual:pool of 3 embryos 8|organism part:embryo|replicate:8|sample name:E MTAB 12934:srpk3 hom ttn1 het 8|scientific name:Danio rerio|sex:not available|strain:T\u00fcpfel long fin", null, null, null, null, null, null, null, null, "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "E MTAB 12934:srpk3 hom ttn1 het 8 p", "srpk3 hom ttn1 het 8 p", "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "Zebrafish embryos were collected for DNA and RNA extraction at 5 dpf in 6 pools of 3 embryos per genotype. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3 M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was then removed without xxx the beads. Whilst still on the magnet  the beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalog number M0303L. NEBNext Ultra II DNA Library Prep Kit for Illumina.", "Experimental Factor: genotype:srpk3  / ; ttn.1 +/ ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP147133", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "ENA FIRST PUBLIC:2024 02 09|ENA LAST UPDATE:2024 02 09", "SLX-21419.H2.HV2TTDRXY.s_2.r_1.fq.gz SLX-21419.H2.HV2TTDRXY.s_2.r_2.fq.gz", "fastq fastq", 4707444000.0, 15691480.0, "E MTAB 12934:SLX 21419.H2.HV2TTDRXY.s 2.r ", "0:150 1:150", "A:1266769556;C:1091810198;G:1114454117;T:1234185729;N:224400", 150, 150, null, null, 1266769556, 1091810198, 1114454117, 1234185729, 224400, "ERX10830015", "ERS15422299", "ERA23329494", "European Bioinformatics Institute|European Nucleotide Archive", "European Bioinformatics Institute|European Nucleotide Archive", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "nebnext", "bulk", "unknown", "unknown", null, "United Kingdom", "2024-02-09", "Larval", "Larval", "Embryo Imprecise", "All anatomical structures"], [11738, "ERR11422850", "ERX10830021", "ERS15422305", "ERP147133", "PRJEB62042", "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "E-MTAB-12934", "Transcriptome Analysis", "RNA seq of zebrafish embryos with mutations in srpk3 sa1890 allele and/or ttn.1 sa5562 allele. Each of the 24 samples six samples for each of four genotypes represents RNA from a pool of three 5 dpf zebrafish embryos.", "ENA FIRST PUBLIC:2024 02 09|ENA LAST UPDATE:2024 02 09", null, "Protocols: Zebrafish embryos were collected for DNA and RNA extraction at 5 dpf in 6 pools of 3 embryos per genotype. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3 M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was then removed without xxx the beads. Whilst still on the magnet  the beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalog number M0303L. NEBNext Ultra II DNA Library Prep Kit for Illumina.", "srpk3 hom ttn1 wt 7", "SAMEA113427179", "QMUL", "ENA FIRST PUBLIC:2024 02 09T16:00:07Z|ENA LAST UPDATE:2024 02 09T16:00:07Z|External Id:SAMEA113427179|INSDC center name:QMUL|INSDC first public:2024 02 09T16:00:07Z|INSDC last update:2024 02 09T16:00:07Z|INSDC status:public|Submitter Id:E MTAB 12934:srpk3 hom ttn1 wt 7|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo|genotype:srpk3  / ; ttn.1 +/+|individual:pool of 3 embryos 7|organism part:embryo|replicate:7|sample name:E MTAB 12934:srpk3 hom ttn1 wt 7|scientific name:Danio rerio|sex:not available|strain:T\u00fcpfel long fin", null, null, null, null, null, null, null, null, "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "E MTAB 12934:srpk3 hom ttn1 wt 7 p", "srpk3 hom ttn1 wt 7 p", "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "Zebrafish embryos were collected for DNA and RNA extraction at 5 dpf in 6 pools of 3 embryos per genotype. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3 M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was then removed without xxx the beads. Whilst still on the magnet  the beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalog number M0303L. NEBNext Ultra II DNA Library Prep Kit for Illumina.", "Experimental Factor: genotype:srpk3  / ; ttn.1 +/+", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP147133", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "ENA FIRST PUBLIC:2024 02 09|ENA LAST UPDATE:2024 02 09", "SLX-21419.G4.HV2TTDRXY.s_2.r_1.fq.gz SLX-21419.G4.HV2TTDRXY.s_2.r_2.fq.gz", "fastq fastq", 6262379700.0, 20874599.0, "E MTAB 12934:SLX 21419.G4.HV2TTDRXY.s 2.r ", "0:150 1:150", "A:1681113096;C:1456657472;G:1485545256;T:1638760380;N:303496", 150, 150, null, null, 1681113096, 1456657472, 1485545256, 1638760380, 303496, "ERX10830021", "ERS15422305", "ERA23329494", "European Bioinformatics Institute|European Nucleotide Archive", "European Bioinformatics Institute|European Nucleotide Archive", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "nebnext", "bulk", "unknown", "unknown", null, "United Kingdom", "2024-02-09", "Larval", "Larval", "Embryo Imprecise", "All anatomical structures"], [11739, "ERR11422846", "ERX10830017", "ERS15422301", "ERP147133", "PRJEB62042", "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "E-MTAB-12934", "Transcriptome Analysis", "RNA seq of zebrafish embryos with mutations in srpk3 sa1890 allele and/or ttn.1 sa5562 allele. Each of the 24 samples six samples for each of four genotypes represents RNA from a pool of three 5 dpf zebrafish embryos.", "ENA FIRST PUBLIC:2024 02 09|ENA LAST UPDATE:2024 02 09", null, "Protocols: Zebrafish embryos were collected for DNA and RNA extraction at 5 dpf in 6 pools of 3 embryos per genotype. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3 M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was then removed without xxx the beads. Whilst still on the magnet  the beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalog number M0303L. NEBNext Ultra II DNA Library Prep Kit for Illumina.", "srpk3 hom ttn1 wt 1", "SAMEA113427175", "QMUL", "ENA FIRST PUBLIC:2024 02 09T16:00:07Z|ENA LAST UPDATE:2024 02 09T16:00:07Z|External Id:SAMEA113427175|INSDC center name:QMUL|INSDC first public:2024 02 09T16:00:07Z|INSDC last update:2024 02 09T16:00:07Z|INSDC status:public|Submitter Id:E MTAB 12934:srpk3 hom ttn1 wt 1|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo|genotype:srpk3  / ; ttn.1 +/+|individual:pool of 3 embryos 1|organism part:embryo|replicate:1|sample name:E MTAB 12934:srpk3 hom ttn1 wt 1|scientific name:Danio rerio|sex:not available|strain:T\u00fcpfel long fin", null, null, null, null, null, null, null, null, "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "E MTAB 12934:srpk3 hom ttn1 wt 1 p", "srpk3 hom ttn1 wt 1 p", "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "Zebrafish embryos were collected for DNA and RNA extraction at 5 dpf in 6 pools of 3 embryos per genotype. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3 M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was then removed without xxx the beads. Whilst still on the magnet  the beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalog number M0303L. NEBNext Ultra II DNA Library Prep Kit for Illumina.", "Experimental Factor: genotype:srpk3  / ; ttn.1 +/+", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP147133", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "ENA FIRST PUBLIC:2024 02 09|ENA LAST UPDATE:2024 02 09", "SLX-21419.A4.HV2TTDRXY.s_2.r_1.fq.gz SLX-21419.A4.HV2TTDRXY.s_2.r_2.fq.gz", "fastq fastq", 6071901300.0, 20239671.0, "E MTAB 12934:SLX 21419.A4.HV2TTDRXY.s 2.r ", "0:150 1:150", "A:1629141748;C:1412963306;G:1441970376;T:1587528201;N:297669", 150, 150, null, null, 1629141748, 1412963306, 1441970376, 1587528201, 297669, "ERX10830017", "ERS15422301", "ERA23329494", "European Bioinformatics Institute|European Nucleotide Archive", "European Bioinformatics Institute|European Nucleotide Archive", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "nebnext", "bulk", "unknown", "unknown", null, "United Kingdom", "2024-02-09", "Larval", "Larval", "Embryo Imprecise", "All anatomical structures"], [11740, "ERR11422860", "ERX10830031", "ERS15422315", "ERP147133", "PRJEB62042", "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "E-MTAB-12934", "Transcriptome Analysis", "RNA seq of zebrafish embryos with mutations in srpk3 sa1890 allele and/or ttn.1 sa5562 allele. Each of the 24 samples six samples for each of four genotypes represents RNA from a pool of three 5 dpf zebrafish embryos.", "ENA FIRST PUBLIC:2024 02 09|ENA LAST UPDATE:2024 02 09", null, "Protocols: Zebrafish embryos were collected for DNA and RNA extraction at 5 dpf in 6 pools of 3 embryos per genotype. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3 M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was then removed without xxx the beads. Whilst still on the magnet  the beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalog number M0303L. NEBNext Ultra II DNA Library Prep Kit for Illumina.", "srpk3 wt ttn1 wt 4", "SAMEA113427189", "QMUL", "ENA FIRST PUBLIC:2024 02 09T16:00:07Z|ENA LAST UPDATE:2024 02 09T16:00:07Z|External Id:SAMEA113427189|INSDC center name:QMUL|INSDC first public:2024 02 09T16:00:07Z|INSDC last update:2024 02 09T16:00:07Z|INSDC status:public|Submitter Id:E MTAB 12934:srpk3 wt ttn1 wt 4|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo|genotype:srpk3 +/+; ttn.1 +/+|individual:pool of 3 embryos 4|organism part:embryo|replicate:4|sample name:E MTAB 12934:srpk3 wt ttn1 wt 4|scientific name:Danio rerio|sex:not available|strain:T\u00fcpfel long fin", null, null, null, null, null, null, null, null, "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "E MTAB 12934:srpk3 wt ttn1 wt 4 p", "srpk3 wt ttn1 wt 4 p", "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "Zebrafish embryos were collected for DNA and RNA extraction at 5 dpf in 6 pools of 3 embryos per genotype. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3 M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was then removed without xxx the beads. Whilst still on the magnet  the beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalog number M0303L. NEBNext Ultra II DNA Library Prep Kit for Illumina.", "Experimental Factor: genotype:srpk3 +/+; ttn.1 +/+", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP147133", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "ENA FIRST PUBLIC:2024 02 09|ENA LAST UPDATE:2024 02 09", "SLX-21419.H4.HV2TTDRXY.s_2.r_1.fq.gz SLX-21419.H4.HV2TTDRXY.s_2.r_2.fq.gz", "fastq fastq", 8552416200.0, 28508054.0, "E MTAB 12934:SLX 21419.H4.HV2TTDRXY.s 2.r ", "0:150 1:150", "A:2292742388;C:1992671634;G:2031906776;T:2234679447;N:415955", 150, 150, null, null, 2292742388, 1992671634, 2031906776, 2234679447, 415955, "ERX10830031", "ERS15422315", "ERA23329494", "European Bioinformatics Institute|European Nucleotide Archive", "European Bioinformatics Institute|European Nucleotide Archive", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "nebnext", "bulk", "unknown", "unknown", null, "United Kingdom", "2024-02-09", "Larval", "Larval", "Embryo Imprecise", "All anatomical structures"], [11741, "ERR11422851", "ERX10830022", "ERS15422306", "ERP147133", "PRJEB62042", "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "E-MTAB-12934", "Transcriptome Analysis", "RNA seq of zebrafish embryos with mutations in srpk3 sa1890 allele and/or ttn.1 sa5562 allele. Each of the 24 samples six samples for each of four genotypes represents RNA from a pool of three 5 dpf zebrafish embryos.", "ENA FIRST PUBLIC:2024 02 09|ENA LAST UPDATE:2024 02 09", null, "Protocols: Zebrafish embryos were collected for DNA and RNA extraction at 5 dpf in 6 pools of 3 embryos per genotype. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3 M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was then removed without xxx the beads. Whilst still on the magnet  the beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalog number M0303L. NEBNext Ultra II DNA Library Prep Kit for Illumina.", "srpk3 hom ttn1 wt 8", "SAMEA113427180", "QMUL", "ENA FIRST PUBLIC:2024 02 09T16:00:07Z|ENA LAST UPDATE:2024 02 09T16:00:07Z|External Id:SAMEA113427180|INSDC center name:QMUL|INSDC first public:2024 02 09T16:00:07Z|INSDC last update:2024 02 09T16:00:07Z|INSDC status:public|Submitter Id:E MTAB 12934:srpk3 hom ttn1 wt 8|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo|genotype:srpk3  / ; ttn.1 +/+|individual:pool of 3 embryos 8|organism part:embryo|replicate:8|sample name:E MTAB 12934:srpk3 hom ttn1 wt 8|scientific name:Danio rerio|sex:not available|strain:T\u00fcpfel long fin", null, null, null, null, null, null, null, null, "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "E MTAB 12934:srpk3 hom ttn1 wt 8 p", "srpk3 hom ttn1 wt 8 p", "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "Zebrafish embryos were collected for DNA and RNA extraction at 5 dpf in 6 pools of 3 embryos per genotype. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3 M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was then removed without xxx the beads. Whilst still on the magnet  the beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalog number M0303L. NEBNext Ultra II DNA Library Prep Kit for Illumina.", "Experimental Factor: genotype:srpk3  / ; ttn.1 +/+", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP147133", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "ENA FIRST PUBLIC:2024 02 09|ENA LAST UPDATE:2024 02 09", "SLX-21419.D4.HV2TTDRXY.s_2.r_1.fq.gz SLX-21419.D4.HV2TTDRXY.s_2.r_2.fq.gz", "fastq fastq", 4693977900.0, 15646593.0, "E MTAB 12934:SLX 21419.D4.HV2TTDRXY.s 2.r ", "0:150 1:150", "A:1260530822;C:1090058889;G:1111595821;T:1231573984;N:218384", 150, 150, null, null, 1260530822, 1090058889, 1111595821, 1231573984, 218384, "ERX10830022", "ERS15422306", "ERA23329494", "European Bioinformatics Institute|European Nucleotide Archive", "European Bioinformatics Institute|European Nucleotide Archive", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "nebnext", "bulk", "unknown", "unknown", null, "United Kingdom", "2024-02-09", "Larval", "Larval", "Embryo Imprecise", "All anatomical structures"], [11742, "ERR11422849", "ERX10830020", "ERS15422304", "ERP147133", "PRJEB62042", "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "E-MTAB-12934", "Transcriptome Analysis", "RNA seq of zebrafish embryos with mutations in srpk3 sa1890 allele and/or ttn.1 sa5562 allele. Each of the 24 samples six samples for each of four genotypes represents RNA from a pool of three 5 dpf zebrafish embryos.", "ENA FIRST PUBLIC:2024 02 09|ENA LAST UPDATE:2024 02 09", null, "Protocols: Zebrafish embryos were collected for DNA and RNA extraction at 5 dpf in 6 pools of 3 embryos per genotype. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3 M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was then removed without xxx the beads. Whilst still on the magnet  the beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalog number M0303L. NEBNext Ultra II DNA Library Prep Kit for Illumina.", "srpk3 hom ttn1 wt 6", "SAMEA113427178", "QMUL", "ENA FIRST PUBLIC:2024 02 09T16:00:07Z|ENA LAST UPDATE:2024 02 09T16:00:07Z|External Id:SAMEA113427178|INSDC center name:QMUL|INSDC first public:2024 02 09T16:00:07Z|INSDC last update:2024 02 09T16:00:07Z|INSDC status:public|Submitter Id:E MTAB 12934:srpk3 hom ttn1 wt 6|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo|genotype:srpk3  / ; ttn.1 +/+|individual:pool of 3 embryos 6|organism part:embryo|replicate:6|sample name:E MTAB 12934:srpk3 hom ttn1 wt 6|scientific name:Danio rerio|sex:not available|strain:T\u00fcpfel long fin", null, null, null, null, null, null, null, null, "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "E MTAB 12934:srpk3 hom ttn1 wt 6 p", "srpk3 hom ttn1 wt 6 p", "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "Zebrafish embryos were collected for DNA and RNA extraction at 5 dpf in 6 pools of 3 embryos per genotype. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3 M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was then removed without xxx the beads. Whilst still on the magnet  the beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalog number M0303L. NEBNext Ultra II DNA Library Prep Kit for Illumina.", "Experimental Factor: genotype:srpk3  / ; ttn.1 +/+", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP147133", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "ENA FIRST PUBLIC:2024 02 09|ENA LAST UPDATE:2024 02 09", "SLX-21419.E2.HV2TTDRXY.s_2.r_1.fq.gz SLX-21419.E2.HV2TTDRXY.s_2.r_2.fq.gz", "fastq fastq", 8212296300.0, 27374321.0, "E MTAB 12934:SLX 21419.E2.HV2TTDRXY.s 2.r ", "0:150 1:150", "A:2208490788;C:1905530286;G:1945454560;T:2152431537;N:389129", 150, 150, null, null, 2208490788, 1905530286, 1945454560, 2152431537, 389129, "ERX10830020", "ERS15422304", "ERA23329494", "European Bioinformatics Institute|European Nucleotide Archive", "European Bioinformatics Institute|European Nucleotide Archive", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "nebnext", "bulk", "unknown", "unknown", null, "United Kingdom", "2024-02-09", "Larval", "Larval", "Embryo Imprecise", "All anatomical structures"], [11743, "ERR11422845", "ERX10830016", "ERS15422300", "ERP147133", "PRJEB62042", "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "E-MTAB-12934", "Transcriptome Analysis", "RNA seq of zebrafish embryos with mutations in srpk3 sa1890 allele and/or ttn.1 sa5562 allele. Each of the 24 samples six samples for each of four genotypes represents RNA from a pool of three 5 dpf zebrafish embryos.", "ENA FIRST PUBLIC:2024 02 09|ENA LAST UPDATE:2024 02 09", null, "Protocols: Zebrafish embryos were collected for DNA and RNA extraction at 5 dpf in 6 pools of 3 embryos per genotype. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3 M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was then removed without xxx the beads. Whilst still on the magnet  the beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalog number M0303L. NEBNext Ultra II DNA Library Prep Kit for Illumina.", "srpk3 hom ttn1 het 9", "SAMEA113427174", "QMUL", "ENA FIRST PUBLIC:2024 02 09T16:00:07Z|ENA LAST UPDATE:2024 02 09T16:00:07Z|External Id:SAMEA113427174|INSDC center name:QMUL|INSDC first public:2024 02 09T16:00:07Z|INSDC last update:2024 02 09T16:00:07Z|INSDC status:public|Submitter Id:E MTAB 12934:srpk3 hom ttn1 het 9|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo|genotype:srpk3  / ; ttn.1 +/ |individual:pool of 3 embryos 9|organism part:embryo|replicate:9|sample name:E MTAB 12934:srpk3 hom ttn1 het 9|scientific name:Danio rerio|sex:not available|strain:T\u00fcpfel long fin", null, null, null, null, null, null, null, null, "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "E MTAB 12934:srpk3 hom ttn1 het 9 p", "srpk3 hom ttn1 het 9 p", "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "Zebrafish embryos were collected for DNA and RNA extraction at 5 dpf in 6 pools of 3 embryos per genotype. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3 M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was then removed without xxx the beads. Whilst still on the magnet  the beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalog number M0303L. NEBNext Ultra II DNA Library Prep Kit for Illumina.", "Experimental Factor: genotype:srpk3  / ; ttn.1 +/ ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP147133", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "ENA FIRST PUBLIC:2024 02 09|ENA LAST UPDATE:2024 02 09", "SLX-21419.H3.HV2TTDRXY.s_2.r_1.fq.gz SLX-21419.H3.HV2TTDRXY.s_2.r_2.fq.gz", "fastq fastq", 6498128700.0, 21660429.0, "E MTAB 12934:SLX 21419.H3.HV2TTDRXY.s 2.r ", "0:150 1:150", "A:1747443944;C:1508729516;G:1535387292;T:1706266342;N:301606", 150, 150, null, null, 1747443944, 1508729516, 1535387292, 1706266342, 301606, "ERX10830016", "ERS15422300", "ERA23329494", "European Bioinformatics Institute|European Nucleotide Archive", "European Bioinformatics Institute|European Nucleotide Archive", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "nebnext", "bulk", "unknown", "unknown", null, "United Kingdom", "2024-02-09", "Larval", "Larval", "Embryo Imprecise", "All anatomical structures"], [11744, "ERR11422861", "ERX10830032", "ERS15422316", "ERP147133", "PRJEB62042", "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "E-MTAB-12934", "Transcriptome Analysis", "RNA seq of zebrafish embryos with mutations in srpk3 sa1890 allele and/or ttn.1 sa5562 allele. Each of the 24 samples six samples for each of four genotypes represents RNA from a pool of three 5 dpf zebrafish embryos.", "ENA FIRST PUBLIC:2024 02 09|ENA LAST UPDATE:2024 02 09", null, "Protocols: Zebrafish embryos were collected for DNA and RNA extraction at 5 dpf in 6 pools of 3 embryos per genotype. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3 M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was then removed without xxx the beads. Whilst still on the magnet  the beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalog number M0303L. NEBNext Ultra II DNA Library Prep Kit for Illumina.", "srpk3 wt ttn1 wt 5", "SAMEA113427190", "QMUL", "ENA FIRST PUBLIC:2024 02 09T16:00:07Z|ENA LAST UPDATE:2024 02 09T16:00:07Z|External Id:SAMEA113427190|INSDC center name:QMUL|INSDC first public:2024 02 09T16:00:07Z|INSDC last update:2024 02 09T16:00:07Z|INSDC status:public|Submitter Id:E MTAB 12934:srpk3 wt ttn1 wt 5|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo|genotype:srpk3 +/+; ttn.1 +/+|individual:pool of 3 embryos 5|organism part:embryo|replicate:5|sample name:E MTAB 12934:srpk3 wt ttn1 wt 5|scientific name:Danio rerio|sex:not available|strain:T\u00fcpfel long fin", null, null, null, null, null, null, null, null, "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "E MTAB 12934:srpk3 wt ttn1 wt 5 p", "srpk3 wt ttn1 wt 5 p", "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "Zebrafish embryos were collected for DNA and RNA extraction at 5 dpf in 6 pools of 3 embryos per genotype. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3 M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was then removed without xxx the beads. Whilst still on the magnet  the beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalog number M0303L. NEBNext Ultra II DNA Library Prep Kit for Illumina.", "Experimental Factor: genotype:srpk3 +/+; ttn.1 +/+", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP147133", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "ENA FIRST PUBLIC:2024 02 09|ENA LAST UPDATE:2024 02 09", "SLX-21419.B5.HV2TTDRXY.s_2.r_1.fq.gz SLX-21419.B5.HV2TTDRXY.s_2.r_2.fq.gz", "fastq fastq", 5670094800.0, 18900316.0, "E MTAB 12934:SLX 21419.B5.HV2TTDRXY.s 2.r ", "0:150 1:150", "A:1520345271;C:1320386106;G:1349261719;T:1479832706;N:268998", 150, 150, null, null, 1520345271, 1320386106, 1349261719, 1479832706, 268998, "ERX10830032", "ERS15422316", "ERA23329494", "European Bioinformatics Institute|European Nucleotide Archive", "European Bioinformatics Institute|European Nucleotide Archive", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "nebnext", "bulk", "unknown", "unknown", null, "United Kingdom", "2024-02-09", "Larval", "Larval", "Embryo Imprecise", "All anatomical structures"], [11745, "ERR11422848", "ERX10830019", "ERS15422303", "ERP147133", "PRJEB62042", "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "E-MTAB-12934", "Transcriptome Analysis", "RNA seq of zebrafish embryos with mutations in srpk3 sa1890 allele and/or ttn.1 sa5562 allele. Each of the 24 samples six samples for each of four genotypes represents RNA from a pool of three 5 dpf zebrafish embryos.", "ENA FIRST PUBLIC:2024 02 09|ENA LAST UPDATE:2024 02 09", null, "Protocols: Zebrafish embryos were collected for DNA and RNA extraction at 5 dpf in 6 pools of 3 embryos per genotype. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3 M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was then removed without xxx the beads. Whilst still on the magnet  the beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalog number M0303L. NEBNext Ultra II DNA Library Prep Kit for Illumina.", "srpk3 hom ttn1 wt 5", "SAMEA113427177", "QMUL", "ENA FIRST PUBLIC:2024 02 09T16:00:07Z|ENA LAST UPDATE:2024 02 09T16:00:07Z|External Id:SAMEA113427177|INSDC center name:QMUL|INSDC first public:2024 02 09T16:00:07Z|INSDC last update:2024 02 09T16:00:07Z|INSDC status:public|Submitter Id:E MTAB 12934:srpk3 hom ttn1 wt 5|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo|genotype:srpk3  / ; ttn.1 +/+|individual:pool of 3 embryos 5|organism part:embryo|replicate:5|sample name:E MTAB 12934:srpk3 hom ttn1 wt 5|scientific name:Danio rerio|sex:not available|strain:T\u00fcpfel long fin", null, null, null, null, null, null, null, null, "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "E MTAB 12934:srpk3 hom ttn1 wt 5 p", "srpk3 hom ttn1 wt 5 p", "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "Zebrafish embryos were collected for DNA and RNA extraction at 5 dpf in 6 pools of 3 embryos per genotype. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3 M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was then removed without xxx the beads. Whilst still on the magnet  the beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalog number M0303L. NEBNext Ultra II DNA Library Prep Kit for Illumina.", "Experimental Factor: genotype:srpk3  / ; ttn.1 +/+", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP147133", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "ENA FIRST PUBLIC:2024 02 09|ENA LAST UPDATE:2024 02 09", "SLX-21419.D3.HV2TTDRXY.s_2.r_1.fq.gz SLX-21419.D3.HV2TTDRXY.s_2.r_2.fq.gz", "fastq fastq", 4627365300.0, 15424551.0, "E MTAB 12934:SLX 21419.D3.HV2TTDRXY.s 2.r ", "0:150 1:150", "A:1240390225;C:1077656611;G:1099301867;T:1209799382;N:217215", 150, 150, null, null, 1240390225, 1077656611, 1099301867, 1209799382, 217215, "ERX10830019", "ERS15422303", "ERA23329494", "European Bioinformatics Institute|European Nucleotide Archive", "European Bioinformatics Institute|European Nucleotide Archive", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "nebnext", "bulk", "unknown", "unknown", null, "United Kingdom", "2024-02-09", "Larval", "Larval", "Embryo Imprecise", "All anatomical structures"], [11746, "ERR11422859", "ERX10830030", "ERS15422314", "ERP147133", "PRJEB62042", "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "E-MTAB-12934", "Transcriptome Analysis", "RNA seq of zebrafish embryos with mutations in srpk3 sa1890 allele and/or ttn.1 sa5562 allele. Each of the 24 samples six samples for each of four genotypes represents RNA from a pool of three 5 dpf zebrafish embryos.", "ENA FIRST PUBLIC:2024 02 09|ENA LAST UPDATE:2024 02 09", null, "Protocols: Zebrafish embryos were collected for DNA and RNA extraction at 5 dpf in 6 pools of 3 embryos per genotype. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3 M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was then removed without xxx the beads. Whilst still on the magnet  the beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalog number M0303L. NEBNext Ultra II DNA Library Prep Kit for Illumina.", "srpk3 wt ttn1 wt 3", "SAMEA113427188", "QMUL", "ENA FIRST PUBLIC:2024 02 09T16:00:07Z|ENA LAST UPDATE:2024 02 09T16:00:07Z|External Id:SAMEA113427188|INSDC center name:QMUL|INSDC first public:2024 02 09T16:00:07Z|INSDC last update:2024 02 09T16:00:07Z|INSDC status:public|Submitter Id:E MTAB 12934:srpk3 wt ttn1 wt 3|age:5|broker name:ArrayExpress|common name:zebrafish|developmental stage:embryo|genotype:srpk3 +/+; ttn.1 +/+|individual:pool of 3 embryos 3|organism part:embryo|replicate:3|sample name:E MTAB 12934:srpk3 wt ttn1 wt 3|scientific name:Danio rerio|sex:not available|strain:T\u00fcpfel long fin", null, null, null, null, null, null, null, null, "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "E MTAB 12934:srpk3 wt ttn1 wt 3 p", "srpk3 wt ttn1 wt 3 p", "RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "Zebrafish embryos were collected for DNA and RNA extraction at 5 dpf in 6 pools of 3 embryos per genotype. Samples were lysed in 110 \u03bcl RLT buffer Qiagen containing 1.1 \u03bcl of 14.3 M \u03b2 mercaptoethanol Sigma. The lysate was allowed to bind to 450 \u03bcl of Agencourt AMPure XP beads Beckman Coulter for 15 minutes. The tubes were left on a magnet Invitrogen until the solutions cleared and the supernatant was then removed without xxx the beads. Whilst still on the magnet  the beads were washed three times with 70% ethanol and allowed to dry for 20 minutes. Total nucleic acid was eluted from the beads following the manufacturer's instructions and treated with DNase I NEB  Catalog number M0303L. NEBNext Ultra II DNA Library Prep Kit for Illumina.", "Experimental Factor: genotype:srpk3 +/+; ttn.1 +/+", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP147133", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of zebrafish embryos with mutations in a muscle specific kinase and the giant titin protein", "ENA FIRST PUBLIC:2024 02 09|ENA LAST UPDATE:2024 02 09", "SLX-21419.F3.HV2TTDRXY.s_2.r_1.fq.gz SLX-21419.F3.HV2TTDRXY.s_2.r_2.fq.gz", "fastq fastq", 6046705800.0, 20155686.0, "E MTAB 12934:SLX 21419.F3.HV2TTDRXY.s 2.r ", "0:150 1:150", "A:1628067770;C:1402636688;G:1430886037;T:1584821803;N:293502", 150, 150, null, null, 1628067770, 1402636688, 1430886037, 1584821803, 293502, "ERX10830030", "ERS15422314", "ERA23329494", "European Bioinformatics Institute|European Nucleotide Archive", "European Bioinformatics Institute|European Nucleotide Archive", null, null, null, null, null, null, null, null, null, null, null, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "nebnext", "bulk", "unknown", "unknown", null, "United Kingdom", "2024-02-09", "Larval", "Larval", "Embryo Imprecise", "All anatomical structures"], [16526, "ERR667398", "ERX622886", "ERS463339", "ERP008536", "PRJEB7614", "Screening zebrafish embryos treated with small molecules by transcriptome profiling", "Screening_zebrafish_embryos_treated_with_small_molecules_by_transcriptome_profiling-sc-3143", "Transcriptome Analysis", "Paired end sequence data from the Illumina HiSeq was prepared from RNA of zebrafish embryos treated with small molecules and their corresponding solvent treated and untreated controls.", null, null, null, null, "SAMEA2548493", "SC", "ArrayExpress Species:Danio rerio|ENA FIRST PUBLIC:2014 11 04T16:19:03Z|ENA LAST UPDATE:2018 03 08T17:42:43Z|External Id:SAMEA2548493|INSDC center name:SC|INSDC first public:2014 11 04T16:19:03Z|INSDC last update:2018 03 08T17:42:43Z|INSDC status:public|Submitter Id:50Cyclop 0 1EtOH 24hpf control 4 sc 1972462|common name:zebrafish|sample description:3 prime end enriched mRNA from single wild type 24hpf embryo treated with 0.1% v/v ethanol.A 8 base indexing sequence TCAGGAGG is bases 13 to 20 of read 1 followed by CG and polyT.|sample name:50Cyclop 0 1EtOH 24hpf control 4 sc 1972462|scientific name:Danio rerio|strain:mixed", null, null, null, null, null, null, null, null, "Illumina HiSeq 2500 paired end sequencing", "SC EXP 13256 1#36", "10517312", "Illumina sequencing of library 10517312  constructed from sample accession ERS463339 for study accession ERP008536.  This is part of an Illumina multiplexed sequencing run 13256 1.  This submission includes reads tagged with the sequence TCAGGAGG.", "qPCR only", null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "ERP008536", "Illumina HiSeq 2500 paired end sequencing", "ENA FIRST PUBLIC:2014 11 04|ENA LAST UPDATE:2018 11 16", "13256_1#36.cram", "cram", 170828190.0, 1314063.0, "SC RUN 13256 1#36", "0:55 1:75", "A:44768263;C:34386047;G:35500778;T:56156162;N:16940", 55, 75, null, null, 44768263, 34386047, 35500778, 56156162, 16940, "ERX622886", "ERS463339", "ERA375333", "SC", "Wellcome Sanger Institute", 2, 0.13166, 0.8204, 0.06755, 0.19006, 0.95753, 0.81655, 0.76236, 0.68533, 55, 75, "T", "B", "mate1 technical by mapping diff", "illumina", "hiseq_era", "3prime", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United Kingdom", "2014-11-04", "Pharyngula", "Embryo", "Embryo Imprecise", "All anatomical structures"], [16527, "ERR667397", "ERX622885", "ERS463338", "ERP008536", "PRJEB7614", "Screening zebrafish embryos treated with small molecules by transcriptome profiling", "Screening_zebrafish_embryos_treated_with_small_molecules_by_transcriptome_profiling-sc-3143", "Transcriptome Analysis", "Paired end sequence data from the Illumina HiSeq was prepared from RNA of zebrafish embryos treated with small molecules and their corresponding solvent treated and untreated controls.", null, null, null, null, "SAMEA2548492", "SC", "ArrayExpress Species:Danio rerio|ENA FIRST PUBLIC:2014 11 04T16:19:03Z|ENA LAST UPDATE:2018 03 08T17:41:34Z|External Id:SAMEA2548492|INSDC center name:SC|INSDC first public:2014 11 04T16:19:03Z|INSDC last update:2018 03 08T17:41:34Z|INSDC status:public|Submitter Id:50Cyclop 0 1EtOH 24hpf control 3 sc 1972461|common name:zebrafish|sample description:3 prime end enriched mRNA from single wild type 24hpf embryo treated with 0.1% v/v ethanol.A 8 base indexing sequence TCTCACGG is bases 13 to 20 of read 1 followed by CG and polyT.|sample name:50Cyclop 0 1EtOH 24hpf control 3 sc 1972461|scientific name:Danio rerio|strain:mixed", null, null, null, null, null, null, null, null, "Illumina HiSeq 2500 paired end sequencing", "SC EXP 13256 1#35", "10517311", "Illumina sequencing of library 10517311  constructed from sample accession ERS463338 for study accession ERP008536.  This is part of an Illumina multiplexed sequencing run 13256 1.  This submission includes reads tagged with the sequence TCTCACGG.", "qPCR only", null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "ERP008536", "Illumina HiSeq 2500 paired end sequencing", "ENA FIRST PUBLIC:2014 11 04|ENA LAST UPDATE:2018 11 16", "13256_1#35.cram", "cram", 261820650.0, 2014005.0, "SC RUN 13256 1#35", "0:55 1:75", "A:72670470;C:50112031;G:54263310;T:84750983;N:23856", 55, 75, null, null, 72670470, 50112031, 54263310, 84750983, 23856, "ERX622885", "ERS463338", "ERA375333", "SC", "Wellcome Sanger Institute", 2, 0.17465, 0.75166, 0.11391, 0.1792, 0.95937, 0.84238, 0.73795, 0.70275, 55, 75, "T", "B", "mate1 technical by mapping diff", "illumina", "hiseq_era", "3prime", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United Kingdom", "2014-11-04", "Pharyngula", "Embryo", "Embryo Imprecise", "All anatomical structures"], [16528, "ERR667396", "ERX622884", "ERS463337", "ERP008536", "PRJEB7614", "Screening zebrafish embryos treated with small molecules by transcriptome profiling", "Screening_zebrafish_embryos_treated_with_small_molecules_by_transcriptome_profiling-sc-3143", "Transcriptome Analysis", "Paired end sequence data from the Illumina HiSeq was prepared from RNA of zebrafish embryos treated with small molecules and their corresponding solvent treated and untreated controls.", null, null, null, null, "SAMEA2548491", "SC", "ArrayExpress Species:Danio rerio|ENA FIRST PUBLIC:2014 11 04T16:19:03Z|ENA LAST UPDATE:2018 03 08T17:42:17Z|External Id:SAMEA2548491|INSDC center name:SC|INSDC first public:2014 11 04T16:19:03Z|INSDC last update:2018 03 08T17:42:17Z|INSDC status:public|Submitter Id:50Cyclop 0 1EtOH 24hpf control 2 sc 1972460|common name:zebrafish|sample description:3 prime end enriched mRNA from single wild type 24hpf embryo treated with 0.1% v/v ethanol.A 8 base indexing sequence TACTTCGG is bases 13 to 20 of read 1 followed by CG and polyT.|sample name:50Cyclop 0 1EtOH 24hpf control 2 sc 1972460|scientific name:Danio rerio|strain:mixed", null, null, null, null, null, null, null, null, "Illumina HiSeq 2500 paired end sequencing", "SC EXP 13256 1#34", "10517310", "Illumina sequencing of library 10517310  constructed from sample accession ERS463337 for study accession ERP008536.  This is part of an Illumina multiplexed sequencing run 13256 1.  This submission includes reads tagged with the sequence TACTTCGG.", "qPCR only", null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "ERP008536", "Illumina HiSeq 2500 paired end sequencing", "ENA FIRST PUBLIC:2014 11 04|ENA LAST UPDATE:2018 11 16", "13256_1#34.cram", "cram", 291378100.0, 2241370.0, "SC RUN 13256 1#34", "0:55 1:75", "A:80660045;C:56367784;G:60328044;T:93993547;N:28680", 55, 75, null, null, 80660045, 56367784, 60328044, 93993547, 28680, "ERX622884", "ERS463337", "ERA375333", "SC", "Wellcome Sanger Institute", 2, 0.16274, 0.76681, 0.10005, 0.16164, 0.95994, 0.84228, 0.77601, 0.72125, 55, 75, "T", "B", "mate1 technical by mapping diff", "illumina", "hiseq_era", "3prime", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United Kingdom", "2014-11-04", "Pharyngula", "Embryo", "Embryo Imprecise", "All anatomical structures"], [16529, "ERR667395", "ERX622883", "ERS463336", "ERP008536", "PRJEB7614", "Screening zebrafish embryos treated with small molecules by transcriptome profiling", "Screening_zebrafish_embryos_treated_with_small_molecules_by_transcriptome_profiling-sc-3143", "Transcriptome Analysis", "Paired end sequence data from the Illumina HiSeq was prepared from RNA of zebrafish embryos treated with small molecules and their corresponding solvent treated and untreated controls.", null, null, null, null, "SAMEA2548490", "SC", "ArrayExpress Species:Danio rerio|ENA FIRST PUBLIC:2014 11 04T16:19:03Z|ENA LAST UPDATE:2018 03 08T17:42:43Z|External Id:SAMEA2548490|INSDC center name:SC|INSDC first public:2014 11 04T16:19:03Z|INSDC last update:2018 03 08T17:42:43Z|INSDC status:public|Submitter Id:50Cyclop 0 1EtOH 24hpf treated 12 sc 1972459|common name:zebrafish|sample description:3 prime end enriched mRNA from single wild type 24hpf embryo treated with 50 micromolar cyclopamine in 0.1% v/v ethanol. A 8 base indexing sequence TGAACTGG is bases 13 to 20 of read 1 followed by CG and polyT.|sample name:50Cyclop 0 1EtOH 24hpf treated 12 sc 1972459|scientific name:Danio rerio|strain:mixed", null, null, null, null, null, null, null, null, "Illumina HiSeq 2500 paired end sequencing", "SC EXP 13256 1#33", "10517309", "Illumina sequencing of library 10517309  constructed from sample accession ERS463336 for study accession ERP008536.  This is part of an Illumina multiplexed sequencing run 13256 1.  This submission includes reads tagged with the sequence TGAACTGG.", "qPCR only", null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "ERP008536", "Illumina HiSeq 2500 paired end sequencing", "ENA FIRST PUBLIC:2014 11 04|ENA LAST UPDATE:2018 11 16", "13256_1#33.cram", "cram", 159495050.0, 1226885.0, "SC RUN 13256 1#33", "0:55 1:75", "A:44526945;C:31107840;G:32400438;T:51444629;N:15198", 55, 75, null, null, 44526945, 31107840, 32400438, 51444629, 15198, "ERX622883", "ERS463336", "ERA375333", "SC", "Wellcome Sanger Institute", 2, 0.14405, 0.72387, 0.09685, 0.16934, 0.96585, 0.84476, 0.75451, 0.71348, 55, 75, "T", "B", "mate1 technical by mapping diff", "illumina", "hiseq_era", "3prime", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United Kingdom", "2014-11-04", "Pharyngula", "Embryo", "Embryo Imprecise", "All anatomical structures"], [16530, "ERR667394", "ERX622882", "ERS463335", "ERP008536", "PRJEB7614", "Screening zebrafish embryos treated with small molecules by transcriptome profiling", "Screening_zebrafish_embryos_treated_with_small_molecules_by_transcriptome_profiling-sc-3143", "Transcriptome Analysis", "Paired end sequence data from the Illumina HiSeq was prepared from RNA of zebrafish embryos treated with small molecules and their corresponding solvent treated and untreated controls.", null, null, null, null, "SAMEA2548489", "SC", "ArrayExpress Species:Danio rerio|ENA FIRST PUBLIC:2014 11 04T16:19:03Z|ENA LAST UPDATE:2018 03 08T17:41:33Z|External Id:SAMEA2548489|INSDC center name:SC|INSDC first public:2014 11 04T16:19:03Z|INSDC last update:2018 03 08T17:41:33Z|INSDC status:public|Submitter Id:50Cyclop 0 1EtOH 24hpf treated 11 sc 1972458|common name:zebrafish|sample description:3 prime end enriched mRNA from single wild type 24hpf embryo treated with 50 micromolar cyclopamine in 0.1% v/v ethanol. A 8 base indexing sequence TTGGTATG is bases 13 to 20 of read 1 followed by CG and polyT.|sample name:50Cyclop 0 1EtOH 24hpf treated 11 sc 1972458|scientific name:Danio rerio|strain:mixed", null, null, null, null, null, null, null, null, "Illumina HiSeq 2500 paired end sequencing", "SC EXP 13256 1#32", "10517308", "Illumina sequencing of library 10517308  constructed from sample accession ERS463335 for study accession ERP008536.  This is part of an Illumina multiplexed sequencing run 13256 1.  This submission includes reads tagged with the sequence TTGGTATG.", "qPCR only", null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "ERP008536", "Illumina HiSeq 2500 paired end sequencing", "ENA FIRST PUBLIC:2014 11 04|ENA LAST UPDATE:2018 11 16", "13256_1#32.cram", "cram", 230733620.0, 1774874.0, "SC RUN 13256 1#32", "0:55 1:75", "A:65923552;C:43534910;G:44813128;T:76440148;N:21882", 55, 75, null, null, 65923552, 43534910, 44813128, 76440148, 21882, "ERX622882", "ERS463335", "ERA375333", "SC", "Wellcome Sanger Institute", 2, 0.16493, 0.71702, 0.10319, 0.16046, 0.96035, 0.83364, 0.7605, 0.69357, 55, 75, "T", "B", "mate1 technical by mapping diff", "illumina", "hiseq_era", "3prime", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United Kingdom", "2014-11-04", "Pharyngula", "Embryo", "Embryo Imprecise", "All anatomical structures"], [16531, "ERR667393", "ERX622881", "ERS463334", "ERP008536", "PRJEB7614", "Screening zebrafish embryos treated with small molecules by transcriptome profiling", "Screening_zebrafish_embryos_treated_with_small_molecules_by_transcriptome_profiling-sc-3143", "Transcriptome Analysis", "Paired end sequence data from the Illumina HiSeq was prepared from RNA of zebrafish embryos treated with small molecules and their corresponding solvent treated and untreated controls.", null, null, null, null, "SAMEA2548488", "SC", "ArrayExpress Species:Danio rerio|ENA FIRST PUBLIC:2014 11 04T16:19:03Z|ENA LAST UPDATE:2018 03 08T17:42:17Z|External Id:SAMEA2548488|INSDC center name:SC|INSDC first public:2014 11 04T16:19:03Z|INSDC last update:2018 03 08T17:42:17Z|INSDC status:public|Submitter Id:50Cyclop 0 1EtOH 24hpf treated 10 sc 1972457|common name:zebrafish|sample description:3 prime end enriched mRNA from single wild type 24hpf embryo treated with 50 micromolar cyclopamine in 0.1% v/v ethanol. A 8 base indexing sequence TAACGCTG is bases 13 to 20 of read 1 followed by CG and polyT.|sample name:50Cyclop 0 1EtOH 24hpf treated 10 sc 1972457|scientific name:Danio rerio|strain:mixed", null, null, null, null, null, null, null, null, "Illumina HiSeq 2500 paired end sequencing", "SC EXP 13256 1#31", "10517307", "Illumina sequencing of library 10517307  constructed from sample accession ERS463334 for study accession ERP008536.  This is part of an Illumina multiplexed sequencing run 13256 1.  This submission includes reads tagged with the sequence TAACGCTG.", "qPCR only", null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "ERP008536", "Illumina HiSeq 2500 paired end sequencing", "ENA FIRST PUBLIC:2014 11 04|ENA LAST UPDATE:2018 11 16", "13256_1#31.cram", "cram", 169693940.0, 1305338.0, "SC RUN 13256 1#31", "0:55 1:75", "A:47216999;C:32233282;G:34303404;T:55924495;N:15760", 55, 75, null, null, 47216999, 32233282, 34303404, 55924495, 15760, "ERX622881", "ERS463334", "ERA375333", "SC", "Wellcome Sanger Institute", 2, 0.15711, 0.76441, 0.09573, 0.15362, 0.96185, 0.83625, 0.78358, 0.43248, 55, 75, "T", "B", "mate1 technical by mapping diff", "illumina", "hiseq_era", "3prime", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United Kingdom", "2014-11-04", "Pharyngula", "Embryo", "Embryo Imprecise", "All anatomical structures"], [16532, "ERR667392", "ERX622880", "ERS463333", "ERP008536", "PRJEB7614", "Screening zebrafish embryos treated with small molecules by transcriptome profiling", "Screening_zebrafish_embryos_treated_with_small_molecules_by_transcriptome_profiling-sc-3143", "Transcriptome Analysis", "Paired end sequence data from the Illumina HiSeq was prepared from RNA of zebrafish embryos treated with small molecules and their corresponding solvent treated and untreated controls.", null, null, null, null, "SAMEA2548487", "SC", "ArrayExpress Species:Danio rerio|ENA FIRST PUBLIC:2014 11 04T16:19:03Z|ENA LAST UPDATE:2018 03 08T17:42:43Z|External Id:SAMEA2548487|INSDC center name:SC|INSDC first public:2014 11 04T16:19:03Z|INSDC last update:2018 03 08T17:42:43Z|INSDC status:public|Submitter Id:50Cyclop 0 1EtOH 24hpf treated 9 sc 1972456|common name:zebrafish|sample description:3 prime end enriched mRNA from single wild type 24hpf embryo treated with 50 micromolar cyclopamine in 0.1% v/v ethanol. A 8 base indexing sequence TCGAAGTG is bases 13 to 20 of read 1 followed by CG and polyT.|sample name:50Cyclop 0 1EtOH 24hpf treated 9 sc 1972456|scientific name:Danio rerio|strain:mixed", null, null, null, null, null, null, null, null, "Illumina HiSeq 2500 paired end sequencing", "SC EXP 13256 1#30", "10517306", "Illumina sequencing of library 10517306  constructed from sample accession ERS463333 for study accession ERP008536.  This is part of an Illumina multiplexed sequencing run 13256 1.  This submission includes reads tagged with the sequence TCGAAGTG.", "qPCR only", null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "ERP008536", "Illumina HiSeq 2500 paired end sequencing", "ENA FIRST PUBLIC:2014 11 04|ENA LAST UPDATE:2018 11 16", "13256_1#30.cram", "cram", 246126400.0, 1893280.0, "SC RUN 13256 1#30", "0:55 1:75", "A:64877434;C:48807745;G:51055789;T:81362490;N:22942", 55, 75, null, null, 64877434, 48807745, 51055789, 81362490, 22942, "ERX622880", "ERS463333", "ERA375333", "SC", "Wellcome Sanger Institute", 2, 0.13616, 0.81131, 0.0854, 0.19973, 0.96556, 0.82757, 0.74775, 0.70258, 55, 75, "T", "B", "mate1 technical by mapping diff", "illumina", "hiseq_era", "3prime", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United Kingdom", "2014-11-04", "Pharyngula", "Embryo", "Embryo Imprecise", "All anatomical structures"], [16533, "ERR667391", "ERX622879", "ERS463332", "ERP008536", "PRJEB7614", "Screening zebrafish embryos treated with small molecules by transcriptome profiling", "Screening_zebrafish_embryos_treated_with_small_molecules_by_transcriptome_profiling-sc-3143", "Transcriptome Analysis", "Paired end sequence data from the Illumina HiSeq was prepared from RNA of zebrafish embryos treated with small molecules and their corresponding solvent treated and untreated controls.", null, null, null, null, "SAMEA2548486", "SC", "ArrayExpress Species:Danio rerio|ENA FIRST PUBLIC:2014 11 04T16:19:03Z|ENA LAST UPDATE:2018 03 08T17:41:33Z|External Id:SAMEA2548486|INSDC center name:SC|INSDC first public:2014 11 04T16:19:03Z|INSDC last update:2018 03 08T17:41:33Z|INSDC status:public|Submitter Id:50Cyclop 0 1EtOH 24hpf treated 8 sc 1972455|common name:zebrafish|sample description:3 prime end enriched mRNA from single wild type 24hpf embryo treated with 50 micromolar cyclopamine in 0.1% v/v ethanol. A 8 base indexing sequence TTCCATTG is bases 13 to 20 of read 1 followed by CG and polyT.|sample name:50Cyclop 0 1EtOH 24hpf treated 8 sc 1972455|scientific name:Danio rerio|strain:mixed", null, null, null, null, null, null, null, null, "Illumina HiSeq 2500 paired end sequencing", "SC EXP 13256 1#29", "10517305", "Illumina sequencing of library 10517305  constructed from sample accession ERS463332 for study accession ERP008536.  This is part of an Illumina multiplexed sequencing run 13256 1.  This submission includes reads tagged with the sequence TTCCATTG.", "qPCR only", null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "ERP008536", "Illumina HiSeq 2500 paired end sequencing", "ENA FIRST PUBLIC:2014 11 04|ENA LAST UPDATE:2018 11 16", "13256_1#29.cram", "cram", 237043690.0, 1823413.0, "SC RUN 13256 1#29", "0:55 1:75", "A:68761865;C:43795810;G:47113828;T:77348945;N:23242", 55, 75, null, null, 68761865, 43795810, 47113828, 77348945, 23242, "ERX622879", "ERS463332", "ERA375333", "SC", "Wellcome Sanger Institute", 2, 0.13798, 0.71442, 0.08126, 0.13596, 0.95712, 0.83751, 0.72277, 0.7226, 55, 75, "T", "B", "mate1 technical by mapping diff", "illumina", "hiseq_era", "3prime", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United Kingdom", "2014-11-04", "Pharyngula", "Embryo", "Embryo Imprecise", "All anatomical structures"], [16534, "ERR667390", "ERX622878", "ERS463331", "ERP008536", "PRJEB7614", "Screening zebrafish embryos treated with small molecules by transcriptome profiling", "Screening_zebrafish_embryos_treated_with_small_molecules_by_transcriptome_profiling-sc-3143", "Transcriptome Analysis", "Paired end sequence data from the Illumina HiSeq was prepared from RNA of zebrafish embryos treated with small molecules and their corresponding solvent treated and untreated controls.", null, null, null, null, "SAMEA2548485", "SC", "ArrayExpress Species:Danio rerio|ENA FIRST PUBLIC:2014 11 04T16:19:03Z|ENA LAST UPDATE:2018 03 08T17:42:17Z|External Id:SAMEA2548485|INSDC center name:SC|INSDC first public:2014 11 04T16:19:03Z|INSDC last update:2018 03 08T17:42:17Z|INSDC status:public|Submitter Id:50Cyclop 0 1EtOH 24hpf treated 7 sc 1972454|common name:zebrafish|sample description:3 prime end enriched mRNA from single wild type 24hpf embryo treated with 50 micromolar cyclopamine in 0.1% v/v ethanol. A 8 base indexing sequence TAGTCTTG is bases 13 to 20 of read 1 followed by CG and polyT.|sample name:50Cyclop 0 1EtOH 24hpf treated 7 sc 1972454|scientific name:Danio rerio|strain:mixed", null, null, null, null, null, null, null, null, "Illumina HiSeq 2500 paired end sequencing", "SC EXP 13256 1#28", "10517304", "Illumina sequencing of library 10517304  constructed from sample accession ERS463331 for study accession ERP008536.  This is part of an Illumina multiplexed sequencing run 13256 1.  This submission includes reads tagged with the sequence TAGTCTTG.", "qPCR only", null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "ERP008536", "Illumina HiSeq 2500 paired end sequencing", "ENA FIRST PUBLIC:2014 11 04|ENA LAST UPDATE:2018 11 16", "13256_1#28.cram", "cram", 394343300.0, 3033410.0, "SC RUN 13256 1#28", "0:55 1:75", "A:111651222;C:75521035;G:80020655;T:127113022;N:37366", 55, 75, null, null, 111651222, 75521035, 80020655, 127113022, 37366, "ERX622878", "ERS463331", "ERA375333", "SC", "Wellcome Sanger Institute", 2, 0.13408, 0.74717, 0.07471, 0.14051, 0.95958, 0.83467, 0.75125, 0.70647, 55, 75, "T", "B", "mate1 technical by mapping diff", "illumina", "hiseq_era", "3prime", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United Kingdom", "2014-11-04", "Pharyngula", "Embryo", "Embryo Imprecise", "All anatomical structures"], [16535, "ERR667389", "ERX622877", "ERS463330", "ERP008536", "PRJEB7614", "Screening zebrafish embryos treated with small molecules by transcriptome profiling", "Screening_zebrafish_embryos_treated_with_small_molecules_by_transcriptome_profiling-sc-3143", "Transcriptome Analysis", "Paired end sequence data from the Illumina HiSeq was prepared from RNA of zebrafish embryos treated with small molecules and their corresponding solvent treated and untreated controls.", null, null, null, null, "SAMEA2548484", "SC", "ArrayExpress Species:Danio rerio|ENA FIRST PUBLIC:2014 11 04T16:19:03Z|ENA LAST UPDATE:2018 03 08T17:42:43Z|External Id:SAMEA2548484|INSDC center name:SC|INSDC first public:2014 11 04T16:19:03Z|INSDC last update:2018 03 08T17:42:43Z|INSDC status:public|Submitter Id:50Cyclop 0 1EtOH 24hpf treated 6 sc 1972453|common name:zebrafish|sample description:3 prime end enriched mRNA from single wild type 24hpf embryo treated with 50 micromolar cyclopamine in 0.1% v/v ethanol. A 8 base indexing sequence TGTGGTTG is bases 13 to 20 of read 1 followed by CG and polyT.|sample name:50Cyclop 0 1EtOH 24hpf treated 6 sc 1972453|scientific name:Danio rerio|strain:mixed", null, null, null, null, null, null, null, null, "Illumina HiSeq 2500 paired end sequencing", "SC EXP 13256 1#27", "10517303", "Illumina sequencing of library 10517303  constructed from sample accession ERS463330 for study accession ERP008536.  This is part of an Illumina multiplexed sequencing run 13256 1.  This submission includes reads tagged with the sequence TGTGGTTG.", "qPCR only", null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "ERP008536", "Illumina HiSeq 2500 paired end sequencing", "ENA FIRST PUBLIC:2014 11 04|ENA LAST UPDATE:2018 11 16", "13256_1#27.cram", "cram", 284205350.0, 2186195.0, "SC RUN 13256 1#27", "0:55 1:75", "A:81760893;C:54722204;G:55386801;T:92306336;N:29116", 55, 75, null, null, 81760893, 54722204, 55386801, 92306336, 29116, "ERX622877", "ERS463330", "ERA375333", "SC", "Wellcome Sanger Institute", 2, 0.15161, 0.71556, 0.08596, 0.14319, 0.95868, 0.8392, 0.77754, 0.71796, 55, 75, "T", "B", "mate1 technical by mapping diff", "illumina", "hiseq_era", "3prime", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United Kingdom", "2014-11-04", "Pharyngula", "Embryo", "Embryo Imprecise", "All anatomical structures"], [16536, "ERR667388", "ERX622876", "ERS463329", "ERP008536", "PRJEB7614", "Screening zebrafish embryos treated with small molecules by transcriptome profiling", "Screening_zebrafish_embryos_treated_with_small_molecules_by_transcriptome_profiling-sc-3143", "Transcriptome Analysis", "Paired end sequence data from the Illumina HiSeq was prepared from RNA of zebrafish embryos treated with small molecules and their corresponding solvent treated and untreated controls.", null, null, null, null, "SAMEA2548483", "SC", "ArrayExpress Species:Danio rerio|ENA FIRST PUBLIC:2014 11 04T16:19:03Z|ENA LAST UPDATE:2018 03 08T17:41:33Z|External Id:SAMEA2548483|INSDC center name:SC|INSDC first public:2014 11 04T16:19:03Z|INSDC last update:2018 03 08T17:41:33Z|INSDC status:public|Submitter Id:50Cyclop 0 1EtOH 24hpf treated 5 sc 1972452|common name:zebrafish|sample description:3 prime end enriched mRNA from single wild type 24hpf embryo treated with 50 micromolar cyclopamine in 0.1% v/v ethanol. A 8 base indexing sequence TCCTCAAT is bases 13 to 20 of read 1 followed by CG and polyT.|sample name:50Cyclop 0 1EtOH 24hpf treated 5 sc 1972452|scientific name:Danio rerio|strain:mixed", null, null, null, null, null, null, null, null, "Illumina HiSeq 2500 paired end sequencing", "SC EXP 13256 1#26", "10517302", "Illumina sequencing of library 10517302  constructed from sample accession ERS463329 for study accession ERP008536.  This is part of an Illumina multiplexed sequencing run 13256 1.  This submission includes reads tagged with the sequence TCCTCAAT.", "qPCR only", null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "ERP008536", "Illumina HiSeq 2500 paired end sequencing", "ENA FIRST PUBLIC:2014 11 04|ENA LAST UPDATE:2018 11 16", "13256_1#26.cram", "cram", 330810350.0, 2544695.0, "SC RUN 13256 1#26", "0:55 1:75", "A:93408226;C:61812207;G:69176898;T:106383919;N:29100", 55, 75, null, null, 93408226, 61812207, 69176898, 106383919, 29100, "ERX622876", "ERS463329", "ERA375333", "SC", "Wellcome Sanger Institute", 2, 0.13377, 0.69577, 0.07532, 0.14875, 0.95781, 0.8383, 0.72303, 0.71175, 55, 75, "T", "B", "mate1 technical by mapping diff", "illumina", "hiseq_era", "3prime", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United Kingdom", "2014-11-04", "Pharyngula", "Embryo", "Embryo Imprecise", "All anatomical structures"], [16537, "ERR667387", "ERX622875", "ERS463328", "ERP008536", "PRJEB7614", "Screening zebrafish embryos treated with small molecules by transcriptome profiling", "Screening_zebrafish_embryos_treated_with_small_molecules_by_transcriptome_profiling-sc-3143", "Transcriptome Analysis", "Paired end sequence data from the Illumina HiSeq was prepared from RNA of zebrafish embryos treated with small molecules and their corresponding solvent treated and untreated controls.", null, null, null, null, "SAMEA2548482", "SC", "ArrayExpress Species:Danio rerio|ENA FIRST PUBLIC:2014 11 04T16:19:03Z|ENA LAST UPDATE:2018 03 08T17:42:16Z|External Id:SAMEA2548482|INSDC center name:SC|INSDC first public:2014 11 04T16:19:03Z|INSDC last update:2018 03 08T17:42:16Z|INSDC status:public|Submitter Id:50Cyclop 0 1EtOH 24hpf treated 4 sc 1972451|common name:zebrafish|sample description:3 prime end enriched mRNA from single wild type 24hpf embryo treated with 50 micromolar cyclopamine in 0.1% v/v ethanol. A 8 base indexing sequence TACAGGAT is bases 13 to 20 of read 1 followed by CG and polyT.|sample name:50Cyclop 0 1EtOH 24hpf treated 4 sc 1972451|scientific name:Danio rerio|strain:mixed", null, null, null, null, null, null, null, null, "Illumina HiSeq 2500 paired end sequencing", "SC EXP 13256 1#25", "10517301", "Illumina sequencing of library 10517301  constructed from sample accession ERS463328 for study accession ERP008536.  This is part of an Illumina multiplexed sequencing run 13256 1.  This submission includes reads tagged with the sequence TACAGGAT.", "qPCR only", null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "ERP008536", "Illumina HiSeq 2500 paired end sequencing", "ENA FIRST PUBLIC:2014 11 04|ENA LAST UPDATE:2018 11 16", "13256_1#25.cram", "cram", 474507020.0, 3650054.0, "SC RUN 13256 1#25", "0:55 1:75", "A:131496123;C:90613341;G:97290951;T:155059153;N:47452", 55, 75, null, null, 131496123, 90613341, 97290951, 155059153, 47452, "ERX622875", "ERS463328", "ERA375333", "SC", "Wellcome Sanger Institute", 2, 0.13262, 0.73888, 0.07276, 0.15006, 0.96451, 0.83479, 0.78366, 0.72049, 55, 75, "T", "B", "mate1 technical by mapping diff", "illumina", "hiseq_era", "3prime", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United Kingdom", "2014-11-04", "Pharyngula", "Embryo", "Embryo Imprecise", "All anatomical structures"], [16550, "ERR667386", "ERX622874", "ERS463327", "ERP008536", "PRJEB7614", "Screening zebrafish embryos treated with small molecules by transcriptome profiling", "Screening_zebrafish_embryos_treated_with_small_molecules_by_transcriptome_profiling-sc-3143", "Transcriptome Analysis", "Paired end sequence data from the Illumina HiSeq was prepared from RNA of zebrafish embryos treated with small molecules and their corresponding solvent treated and untreated controls.", null, null, null, null, "SAMEA2548481", "SC", "ArrayExpress Species:Danio rerio|ENA FIRST PUBLIC:2014 11 04T16:19:03Z|ENA LAST UPDATE:2018 03 08T17:42:43Z|External Id:SAMEA2548481|INSDC center name:SC|INSDC first public:2014 11 04T16:19:03Z|INSDC last update:2018 03 08T17:42:43Z|INSDC status:public|Submitter Id:50Cyclop 0 1EtOH 24hpf treated 3 sc 1972450|common name:zebrafish|sample description:3 prime end enriched mRNA from single wild type 24hpf embryo treated with 50 micromolar cyclopamine in 0.1% v/v ethanol. A 8 base indexing sequence TAGTGACT is bases 13 to 20 of read 1 followed by CG and polyT.|sample name:50Cyclop 0 1EtOH 24hpf treated 3 sc 1972450|scientific name:Danio rerio|strain:mixed", null, null, null, null, null, null, null, null, "Illumina HiSeq 2500 paired end sequencing", "SC EXP 13256 1#24", "10517300", "Illumina sequencing of library 10517300  constructed from sample accession ERS463327 for study accession ERP008536.  This is part of an Illumina multiplexed sequencing run 13256 1.  This submission includes reads tagged with the sequence TAGTGACT.", "qPCR only", null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "ERP008536", "Illumina HiSeq 2500 paired end sequencing", "ENA FIRST PUBLIC:2014 11 04|ENA LAST UPDATE:2018 11 16", "13256_1#24.cram", "cram", 296075650.0, 2277505.0, "SC RUN 13256 1#24", "0:55 1:75", "A:82935889;C:56062979;G:59762388;T:97286073;N:28321", 55, 75, null, null, 82935889, 56062979, 59762388, 97286073, 28321, "ERX622874", "ERS463327", "ERA375333", "SC", "Wellcome Sanger Institute", 2, 0.1437, 0.74103, 0.08347, 0.14393, 0.96325, 0.83684, 0.81783, 0.70771, 55, 75, "T", "B", "mate1 technical by mapping diff", "illumina", "hiseq_era", "3prime", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United Kingdom", "2014-11-04", "Pharyngula", "Embryo", "Embryo Imprecise", "All anatomical structures"], [16551, "ERR667385", "ERX622873", "ERS463326", "ERP008536", "PRJEB7614", "Screening zebrafish embryos treated with small molecules by transcriptome profiling", "Screening_zebrafish_embryos_treated_with_small_molecules_by_transcriptome_profiling-sc-3143", "Transcriptome Analysis", "Paired end sequence data from the Illumina HiSeq was prepared from RNA of zebrafish embryos treated with small molecules and their corresponding solvent treated and untreated controls.", null, null, null, null, "SAMEA2548480", "SC", "ArrayExpress Species:Danio rerio|ENA FIRST PUBLIC:2014 11 04T16:19:03Z|ENA LAST UPDATE:2018 03 08T17:41:33Z|External Id:SAMEA2548480|INSDC center name:SC|INSDC first public:2014 11 04T16:19:03Z|INSDC last update:2018 03 08T17:41:33Z|INSDC status:public|Submitter Id:50Cyclop 0 1EtOH 24hpf treated 2 sc 1972449|common name:zebrafish|sample description:3 prime end enriched mRNA from single wild type 24hpf embryo treated with 50 micromolar cyclopamine in 0.1% v/v ethanol. A 8 base indexing sequence TTCCTGCT is bases 13 to 20 of read 1 followed by CG and polyT.|sample name:50Cyclop 0 1EtOH 24hpf treated 2 sc 1972449|scientific name:Danio rerio|strain:mixed", null, null, null, null, null, null, null, null, "Illumina HiSeq 2500 paired end sequencing", "SC EXP 13256 1#23", "10517299", "Illumina sequencing of library 10517299  constructed from sample accession ERS463326 for study accession ERP008536.  This is part of an Illumina multiplexed sequencing run 13256 1.  This submission includes reads tagged with the sequence TTCCTGCT.", "qPCR only", null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "ERP008536", "Illumina HiSeq 2500 paired end sequencing", "ENA FIRST PUBLIC:2014 11 04|ENA LAST UPDATE:2018 11 16", "13256_1#23.cram", "cram", 443750710.0, 3413467.0, "SC RUN 13256 1#23", "0:55 1:75", "A:124952195;C:85586617;G:94833109;T:138341796;N:36993", 55, 75, null, null, 124952195, 85586617, 94833109, 138341796, 36993, "ERX622873", "ERS463326", "ERA375333", "SC", "Wellcome Sanger Institute", 2, 0.15245, 0.72135, 0.07368, 0.15151, 0.95568, 0.84449, 0.77081, 0.72424, 55, 75, "T", "B", "mate1 technical by mapping diff", "illumina", "hiseq_era", "3prime", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United Kingdom", "2014-11-04", "Pharyngula", "Embryo", "Embryo Imprecise", "All anatomical structures"], [16552, "ERR667384", "ERX622872", "ERS463325", "ERP008536", "PRJEB7614", "Screening zebrafish embryos treated with small molecules by transcriptome profiling", "Screening_zebrafish_embryos_treated_with_small_molecules_by_transcriptome_profiling-sc-3143", "Transcriptome Analysis", "Paired end sequence data from the Illumina HiSeq was prepared from RNA of zebrafish embryos treated with small molecules and their corresponding solvent treated and untreated controls.", null, null, null, null, "SAMEA2548479", "SC", "ArrayExpress Species:Danio rerio|ENA FIRST PUBLIC:2014 11 04T16:19:03Z|ENA LAST UPDATE:2018 03 08T17:42:16Z|External Id:SAMEA2548479|INSDC center name:SC|INSDC first public:2014 11 04T16:19:03Z|INSDC last update:2018 03 08T17:42:16Z|INSDC status:public|Submitter Id:50Cyclop 0 1EtOH 24hpf treated 1 sc 1972448|common name:zebrafish|sample description:3 prime end enriched mRNA from single wild type 24hpf embryo treated with 50 micromolar cyclopamine in 0.1% v/v ethanol. A 8 base indexing sequence TGCGATCT is bases 13 to 20 of read 1 followed by CG and polyT.|sample name:50Cyclop 0 1EtOH 24hpf treated 1 sc 1972448|scientific name:Danio rerio|strain:mixed", null, null, null, null, null, null, null, null, "Illumina HiSeq 2500 paired end sequencing", "SC EXP 13256 1#22", "10517298", "Illumina sequencing of library 10517298  constructed from sample accession ERS463325 for study accession ERP008536.  This is part of an Illumina multiplexed sequencing run 13256 1.  This submission includes reads tagged with the sequence TGCGATCT.", "qPCR only", null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "ERP008536", "Illumina HiSeq 2500 paired end sequencing", "ENA FIRST PUBLIC:2014 11 04|ENA LAST UPDATE:2018 11 16", "13256_1#22.cram", "cram", 298785370.0, 2298349.0, "SC RUN 13256 1#22", "0:55 1:75", "A:82163853;C:59663280;G:63342361;T:93589112;N:26764", 55, 75, null, null, 82163853, 59663280, 63342361, 93589112, 26764, "ERX622872", "ERS463325", "ERA375333", "SC", "Wellcome Sanger Institute", 2, 0.13397, 0.72614, 0.07209, 0.16108, 0.96303, 0.85188, 0.78255, 0.73973, 55, 75, "T", "B", "mate1 technical by mapping diff", "illumina", "hiseq_era", "3prime", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United Kingdom", "2014-11-04", "Pharyngula", "Embryo", "Embryo Imprecise", "All anatomical structures"], [16553, "ERR667383", "ERX622871", "ERS463324", "ERP008536", "PRJEB7614", "Screening zebrafish embryos treated with small molecules by transcriptome profiling", "Screening_zebrafish_embryos_treated_with_small_molecules_by_transcriptome_profiling-sc-3143", "Transcriptome Analysis", "Paired end sequence data from the Illumina HiSeq was prepared from RNA of zebrafish embryos treated with small molecules and their corresponding solvent treated and untreated controls.", null, null, null, null, "SAMEA2548478", "SC", "ArrayExpress Species:Danio rerio|ENA FIRST PUBLIC:2014 11 04T16:19:03Z|ENA LAST UPDATE:2018 03 08T17:42:43Z|External Id:SAMEA2548478|INSDC center name:SC|INSDC first public:2014 11 04T16:19:03Z|INSDC last update:2018 03 08T17:42:43Z|INSDC status:public|Submitter Id:25Cyclop 05EtOH 24hpf control 12 sc 1972447|common name:zebrafish|sample description:3 prime end enriched mRNA from single wild type 24hpf embryo treated with 05% v/v ethanol.A 8 base indexing sequence TTGACTCT is bases 13 to 20 of read 1 followed by CG and polyT.|sample name:25Cyclop 05EtOH 24hpf control 12 sc 1972447|scientific name:Danio rerio|strain:mixed", null, null, null, null, null, null, null, null, "Illumina HiSeq 2500 paired end sequencing", "SC EXP 13256 1#21", "10517297", "Illumina sequencing of library 10517297  constructed from sample accession ERS463324 for study accession ERP008536.  This is part of an Illumina multiplexed sequencing run 13256 1.  This submission includes reads tagged with the sequence TTGACTCT.", "qPCR only", null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "ERP008536", "Illumina HiSeq 2500 paired end sequencing", "ENA FIRST PUBLIC:2014 11 04|ENA LAST UPDATE:2018 11 16", "13256_1#21.cram", "cram", 140774920.0, 1082884.0, "SC RUN 13256 1#21", "0:55 1:75", "A:39481115;C:27348433;G:30029240;T:43903005;N:13127", 55, 75, null, null, 39481115, 27348433, 30029240, 43903005, 13127, "ERX622871", "ERS463324", "ERA375333", "SC", "Wellcome Sanger Institute", 2, 0.14879, 0.72247, 0.08143, 0.15522, 0.96118, 0.84867, 0.78965, 0.74254, 55, 75, "T", "B", "mate1 technical by mapping diff", "illumina", "hiseq_era", "3prime", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United Kingdom", "2014-11-04", "Pharyngula", "Embryo", "Embryo Imprecise", "All anatomical structures"], [16554, "ERR667382", "ERX622870", "ERS463323", "ERP008536", "PRJEB7614", "Screening zebrafish embryos treated with small molecules by transcriptome profiling", "Screening_zebrafish_embryos_treated_with_small_molecules_by_transcriptome_profiling-sc-3143", "Transcriptome Analysis", "Paired end sequence data from the Illumina HiSeq was prepared from RNA of zebrafish embryos treated with small molecules and their corresponding solvent treated and untreated controls.", null, null, null, null, "SAMEA2548477", "SC", "ArrayExpress Species:Danio rerio|ENA FIRST PUBLIC:2014 11 04T16:19:03Z|ENA LAST UPDATE:2018 03 08T17:41:33Z|External Id:SAMEA2548477|INSDC center name:SC|INSDC first public:2014 11 04T16:19:03Z|INSDC last update:2018 03 08T17:41:33Z|INSDC status:public|Submitter Id:25Cyclop 05EtOH 24hpf control 11 sc 1972446|common name:zebrafish|sample description:3 prime end enriched mRNA from single wild type 24hpf embryo treated with 05% v/v ethanol.A 8 base indexing sequence TGCATAGT is bases 13 to 20 of read 1 followed by CG and polyT.|sample name:25Cyclop 05EtOH 24hpf control 11 sc 1972446|scientific name:Danio rerio|strain:mixed", null, null, null, null, null, null, null, null, "Illumina HiSeq 2500 paired end sequencing", "SC EXP 13256 1#20", "10517296", "Illumina sequencing of library 10517296  constructed from sample accession ERS463323 for study accession ERP008536.  This is part of an Illumina multiplexed sequencing run 13256 1.  This submission includes reads tagged with the sequence TGCATAGT.", "qPCR only", null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "ERP008536", "Illumina HiSeq 2500 paired end sequencing", "ENA FIRST PUBLIC:2014 11 04|ENA LAST UPDATE:2018 11 16", "13256_1#20.cram", "cram", 178794590.0, 1375343.0, "SC RUN 13256 1#20", "0:55 1:75", "A:50890564;C:34618169;G:37008888;T:56258907;N:18062", 55, 75, null, null, 50890564, 34618169, 37008888, 56258907, 18062, "ERX622870", "ERS463323", "ERA375333", "SC", "Wellcome Sanger Institute", 2, 0.15828, 0.68013, 0.10522, 0.16411, 0.96301, 0.85147, 0.77788, 0.71946, 55, 75, "T", "B", "mate1 technical by mapping diff", "illumina", "hiseq_era", "3prime", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United Kingdom", "2014-11-04", "Pharyngula", "Embryo", "Embryo Imprecise", "All anatomical structures"], [16555, "ERR667381", "ERX622869", "ERS463322", "ERP008536", "PRJEB7614", "Screening zebrafish embryos treated with small molecules by transcriptome profiling", "Screening_zebrafish_embryos_treated_with_small_molecules_by_transcriptome_profiling-sc-3143", "Transcriptome Analysis", "Paired end sequence data from the Illumina HiSeq was prepared from RNA of zebrafish embryos treated with small molecules and their corresponding solvent treated and untreated controls.", null, null, null, null, "SAMEA2548476", "SC", "ArrayExpress Species:Danio rerio|ENA FIRST PUBLIC:2014 11 04T16:19:03Z|ENA LAST UPDATE:2018 03 08T17:42:16Z|External Id:SAMEA2548476|INSDC center name:SC|INSDC first public:2014 11 04T16:19:03Z|INSDC last update:2018 03 08T17:42:16Z|INSDC status:public|Submitter Id:25Cyclop 05EtOH 24hpf control 10 sc 1972445|common name:zebrafish|sample description:3 prime end enriched mRNA from single wild type 24hpf embryo treated with 05% v/v ethanol.A 8 base indexing sequence TGATACGT is bases 13 to 20 of read 1 followed by CG and polyT.|sample name:25Cyclop 05EtOH 24hpf control 10 sc 1972445|scientific name:Danio rerio|strain:mixed", null, null, null, null, null, null, null, null, "Illumina HiSeq 2500 paired end sequencing", "SC EXP 13256 1#19", "10517295", "Illumina sequencing of library 10517295  constructed from sample accession ERS463322 for study accession ERP008536.  This is part of an Illumina multiplexed sequencing run 13256 1.  This submission includes reads tagged with the sequence TGATACGT.", "qPCR only", null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "ERP008536", "Illumina HiSeq 2500 paired end sequencing", "ENA FIRST PUBLIC:2014 11 04|ENA LAST UPDATE:2018 11 16", "13256_1#19.cram", "cram", 152796020.0, 1175354.0, "SC RUN 13256 1#19", "0:55 1:75", "A:43164178;C:28497500;G:30636978;T:50482443;N:14921", 55, 75, null, null, 43164178, 28497500, 30636978, 50482443, 14921, "ERX622869", "ERS463322", "ERA375333", "SC", "Wellcome Sanger Institute", 2, 0.17338, 0.74214, 0.11412, 0.17188, 0.95954, 0.83475, 0.77599, 0.69646, 55, 75, "T", "B", "mate1 technical by mapping diff", "illumina", "hiseq_era", "3prime", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United Kingdom", "2014-11-04", "Pharyngula", "Embryo", "Embryo Imprecise", "All anatomical structures"], [16556, "ERR667380", "ERX622868", "ERS463321", "ERP008536", "PRJEB7614", "Screening zebrafish embryos treated with small molecules by transcriptome profiling", "Screening_zebrafish_embryos_treated_with_small_molecules_by_transcriptome_profiling-sc-3143", "Transcriptome Analysis", "Paired end sequence data from the Illumina HiSeq was prepared from RNA of zebrafish embryos treated with small molecules and their corresponding solvent treated and untreated controls.", null, null, null, null, "SAMEA2548475", "SC", "ArrayExpress Species:Danio rerio|ENA FIRST PUBLIC:2014 11 04T16:19:03Z|ENA LAST UPDATE:2018 03 08T17:42:43Z|External Id:SAMEA2548475|INSDC center name:SC|INSDC first public:2014 11 04T16:19:03Z|INSDC last update:2018 03 08T17:42:43Z|INSDC status:public|Submitter Id:25Cyclop 05EtOH 24hpf control 9 sc 1972444|common name:zebrafish|sample description:3 prime end enriched mRNA from single wild type 24hpf embryo treated with 05% v/v ethanol.A 8 base indexing sequence TCGAGCGT is bases 13 to 20 of read 1 followed by CG and polyT.|sample name:25Cyclop 05EtOH 24hpf control 9 sc 1972444|scientific name:Danio rerio|strain:mixed", null, null, null, null, null, null, null, null, "Illumina HiSeq 2500 paired end sequencing", "SC EXP 13256 1#18", "10517294", "Illumina sequencing of library 10517294  constructed from sample accession ERS463321 for study accession ERP008536.  This is part of an Illumina multiplexed sequencing run 13256 1.  This submission includes reads tagged with the sequence TCGAGCGT.", "qPCR only", null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "ERP008536", "Illumina HiSeq 2500 paired end sequencing", "ENA FIRST PUBLIC:2014 11 04|ENA LAST UPDATE:2018 11 16", "13256_1#18.cram", "cram", 246614290.0, 1897033.0, "SC RUN 13256 1#18", "0:55 1:75", "A:65847226;C:48649826;G:51891861;T:80201978;N:23399", 55, 75, null, null, 65847226, 48649826, 51891861, 80201978, 23399, "ERX622868", "ERS463321", "ERA375333", "SC", "Wellcome Sanger Institute", 2, 0.12616, 0.80609, 0.07386, 0.19379, 0.96566, 0.82942, 0.80786, 0.70582, 55, 75, "T", "B", "mate1 technical by mapping diff", "illumina", "hiseq_era", "3prime", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United Kingdom", "2014-11-04", "Pharyngula", "Embryo", "Embryo Imprecise", "All anatomical structures"], [16557, "ERR667379", "ERX622867", "ERS463320", "ERP008536", "PRJEB7614", "Screening zebrafish embryos treated with small molecules by transcriptome profiling", "Screening_zebrafish_embryos_treated_with_small_molecules_by_transcriptome_profiling-sc-3143", "Transcriptome Analysis", "Paired end sequence data from the Illumina HiSeq was prepared from RNA of zebrafish embryos treated with small molecules and their corresponding solvent treated and untreated controls.", null, null, null, null, "SAMEA2548474", "SC", "ArrayExpress Species:Danio rerio|ENA FIRST PUBLIC:2014 11 04T16:19:03Z|ENA LAST UPDATE:2018 03 08T17:41:33Z|External Id:SAMEA2548474|INSDC center name:SC|INSDC first public:2014 11 04T16:19:03Z|INSDC last update:2018 03 08T17:41:33Z|INSDC status:public|Submitter Id:25Cyclop 05EtOH 24hpf control 8 sc 1972443|common name:zebrafish|sample description:3 prime end enriched mRNA from single wild type 24hpf embryo treated with 05% v/v ethanol.A 8 base indexing sequence TTGGAGGT is bases 13 to 20 of read 1 followed by CG and polyT.|sample name:25Cyclop 05EtOH 24hpf control 8 sc 1972443|scientific name:Danio rerio|strain:mixed", null, null, null, null, null, null, null, null, "Illumina HiSeq 2500 paired end sequencing", "SC EXP 13256 1#17", "10517293", "Illumina sequencing of library 10517293  constructed from sample accession ERS463320 for study accession ERP008536.  This is part of an Illumina multiplexed sequencing run 13256 1.  This submission includes reads tagged with the sequence TTGGAGGT.", "qPCR only", null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "ERP008536", "Illumina HiSeq 2500 paired end sequencing", "ENA FIRST PUBLIC:2014 11 04|ENA LAST UPDATE:2018 11 16", "13256_1#17.cram", "cram", 192037820.0, 1477214.0, "SC RUN 13256 1#17", "0:55 1:75", "A:52262399;C:38034317;G:39394465;T:62328424;N:18215", 55, 75, null, null, 52262399, 38034317, 39394465, 62328424, 18215, "ERX622867", "ERS463320", "ERA375333", "SC", "Wellcome Sanger Institute", 2, 0.13419, 0.77104, 0.06619, 0.15595, 0.96234, 0.83431, 0.80856, 0.7337, 55, 75, "T", "B", "mate1 technical by mapping diff", "illumina", "hiseq_era", "3prime", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United Kingdom", "2014-11-04", "Pharyngula", "Embryo", "Embryo Imprecise", "All anatomical structures"], [16558, "ERR667378", "ERX622866", "ERS463319", "ERP008536", "PRJEB7614", "Screening zebrafish embryos treated with small molecules by transcriptome profiling", "Screening_zebrafish_embryos_treated_with_small_molecules_by_transcriptome_profiling-sc-3143", "Transcriptome Analysis", "Paired end sequence data from the Illumina HiSeq was prepared from RNA of zebrafish embryos treated with small molecules and their corresponding solvent treated and untreated controls.", null, null, null, null, "SAMEA2548473", "SC", "ArrayExpress Species:Danio rerio|ENA FIRST PUBLIC:2014 11 04T16:19:03Z|ENA LAST UPDATE:2018 03 08T17:42:16Z|External Id:SAMEA2548473|INSDC center name:SC|INSDC first public:2014 11 04T16:19:03Z|INSDC last update:2018 03 08T17:42:16Z|INSDC status:public|Submitter Id:25Cyclop 05EtOH 24hpf control 7 sc 1972442|common name:zebrafish|sample description:3 prime end enriched mRNA from single wild type 24hpf embryo treated with 05% v/v ethanol.A 8 base indexing sequence TCTGCTGT is bases 13 to 20 of read 1 followed by CG and polyT.|sample name:25Cyclop 05EtOH 24hpf control 7 sc 1972442|scientific name:Danio rerio|strain:mixed", null, null, null, null, null, null, null, null, "Illumina HiSeq 2500 paired end sequencing", "SC EXP 13256 1#16", "10517292", "Illumina sequencing of library 10517292  constructed from sample accession ERS463319 for study accession ERP008536.  This is part of an Illumina multiplexed sequencing run 13256 1.  This submission includes reads tagged with the sequence TCTGCTGT.", "qPCR only", null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "ERP008536", "Illumina HiSeq 2500 paired end sequencing", "ENA FIRST PUBLIC:2014 11 04|ENA LAST UPDATE:2018 11 16", "13256_1#16.cram", "cram", 131783210.0, 1013717.0, "SC RUN 13256 1#16", "0:55 1:75", "A:36740307;C:25906782;G:28184476;T:40940400;N:11245", 55, 75, null, null, 36740307, 25906782, 28184476, 40940400, 11245, "ERX622866", "ERS463319", "ERA375333", "SC", "Wellcome Sanger Institute", 2, 0.1814, 0.75096, 0.0779, 0.15257, 0.94408, 0.83615, 0.73999, 0.70358, 55, 75, "T", "B", "mate1 technical by mapping diff", "illumina", "hiseq_era", "3prime", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United Kingdom", "2014-11-04", "Pharyngula", "Embryo", "Embryo Imprecise", "All anatomical structures"], [16559, "ERR667377", "ERX622865", "ERS463318", "ERP008536", "PRJEB7614", "Screening zebrafish embryos treated with small molecules by transcriptome profiling", "Screening_zebrafish_embryos_treated_with_small_molecules_by_transcriptome_profiling-sc-3143", "Transcriptome Analysis", "Paired end sequence data from the Illumina HiSeq was prepared from RNA of zebrafish embryos treated with small molecules and their corresponding solvent treated and untreated controls.", null, null, null, null, "SAMEA2548472", "SC", "ArrayExpress Species:Danio rerio|ENA FIRST PUBLIC:2014 11 04T16:19:03Z|ENA LAST UPDATE:2018 03 08T17:42:43Z|External Id:SAMEA2548472|INSDC center name:SC|INSDC first public:2014 11 04T16:19:03Z|INSDC last update:2018 03 08T17:42:43Z|INSDC status:public|Submitter Id:25Cyclop 05EtOH 24hpf control 6 sc 1972441|common name:zebrafish|sample description:3 prime end enriched mRNA from single wild type 24hpf embryo treated with 05% v/v ethanol.A 8 base indexing sequence TTCTGTGT is bases 13 to 20 of read 1 followed by CG and polyT.|sample name:25Cyclop 05EtOH 24hpf control 6 sc 1972441|scientific name:Danio rerio|strain:mixed", null, null, null, null, null, null, null, null, "Illumina HiSeq 2500 paired end sequencing", "SC EXP 13256 1#15", "10517291", "Illumina sequencing of library 10517291  constructed from sample accession ERS463318 for study accession ERP008536.  This is part of an Illumina multiplexed sequencing run 13256 1.  This submission includes reads tagged with the sequence TTCTGTGT.", "qPCR only", null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "ERP008536", "Illumina HiSeq 2500 paired end sequencing", "ENA FIRST PUBLIC:2014 11 04|ENA LAST UPDATE:2018 11 16", "13256_1#15.cram", "cram", 297275420.0, 2286734.0, "SC RUN 13256 1#15", "0:55 1:75", "A:80591742;C:58460335;G:62097495;T:96099260;N:26588", 55, 75, null, null, 80591742, 58460335, 62097495, 96099260, 26588, "ERX622865", "ERS463318", "ERA375333", "SC", "Wellcome Sanger Institute", 2, 0.14856, 0.79328, 0.08045, 0.18298, 0.95388, 0.82524, 0.74068, 0.69319, 55, 75, "T", "B", "mate1 technical by mapping diff", "illumina", "hiseq_era", "3prime", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United Kingdom", "2014-11-04", "Pharyngula", "Embryo", "Embryo Imprecise", "All anatomical structures"], [16560, "ERR667376", "ERX622864", "ERS463317", "ERP008536", "PRJEB7614", "Screening zebrafish embryos treated with small molecules by transcriptome profiling", "Screening_zebrafish_embryos_treated_with_small_molecules_by_transcriptome_profiling-sc-3143", "Transcriptome Analysis", "Paired end sequence data from the Illumina HiSeq was prepared from RNA of zebrafish embryos treated with small molecules and their corresponding solvent treated and untreated controls.", null, null, null, null, "SAMEA2548471", "SC", "ArrayExpress Species:Danio rerio|ENA FIRST PUBLIC:2014 11 04T16:19:03Z|ENA LAST UPDATE:2018 03 08T17:41:33Z|External Id:SAMEA2548471|INSDC center name:SC|INSDC first public:2014 11 04T16:19:03Z|INSDC last update:2018 03 08T17:41:33Z|INSDC status:public|Submitter Id:25Cyclop 05EtOH 24hpf control 5 sc 1972440|common name:zebrafish|sample description:3 prime end enriched mRNA from single wild type 24hpf embryo treated with 05% v/v ethanol.A 8 base indexing sequence TGTACCTT is bases 13 to 20 of read 1 followed by CG and polyT.|sample name:25Cyclop 05EtOH 24hpf control 5 sc 1972440|scientific name:Danio rerio|strain:mixed", null, null, null, null, null, null, null, null, "Illumina HiSeq 2500 paired end sequencing", "SC EXP 13256 1#14", "10517290", "Illumina sequencing of library 10517290  constructed from sample accession ERS463317 for study accession ERP008536.  This is part of an Illumina multiplexed sequencing run 13256 1.  This submission includes reads tagged with the sequence TGTACCTT.", "qPCR only", null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "ERP008536", "Illumina HiSeq 2500 paired end sequencing", "ENA FIRST PUBLIC:2014 11 04|ENA LAST UPDATE:2018 11 16", "13256_1#14.cram", "cram", 242173750.0, 1862875.0, "SC RUN 13256 1#14", "0:55 1:75", "A:72439393;C:44966903;G:51788847;T:72956754;N:21853", 55, 75, null, null, 72439393, 44966903, 51788847, 72956754, 21853, "ERX622864", "ERS463317", "ERA375333", "SC", "Wellcome Sanger Institute", 2, 0.13638, 0.61957, 0.09812, 0.16019, 0.97009, 0.87127, 0.57527, 0.73119, 55, 75, "T", "B", "mate1 technical by mapping diff", "illumina", "hiseq_era", "3prime", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United Kingdom", "2014-11-04", "Pharyngula", "Embryo", "Embryo Imprecise", "All anatomical structures"], [16561, "ERR667375", "ERX622863", "ERS463316", "ERP008536", "PRJEB7614", "Screening zebrafish embryos treated with small molecules by transcriptome profiling", "Screening_zebrafish_embryos_treated_with_small_molecules_by_transcriptome_profiling-sc-3143", "Transcriptome Analysis", "Paired end sequence data from the Illumina HiSeq was prepared from RNA of zebrafish embryos treated with small molecules and their corresponding solvent treated and untreated controls.", null, null, null, null, "SAMEA2548470", "SC", "ArrayExpress Species:Danio rerio|ENA FIRST PUBLIC:2014 11 04T16:19:03Z|ENA LAST UPDATE:2018 03 08T17:42:16Z|External Id:SAMEA2548470|INSDC center name:SC|INSDC first public:2014 11 04T16:19:03Z|INSDC last update:2018 03 08T17:42:16Z|INSDC status:public|Submitter Id:25Cyclop 05EtOH 24hpf control 4 sc 1972439|common name:zebrafish|sample description:3 prime end enriched mRNA from single wild type 24hpf embryo treated with 05% v/v ethanol.A 8 base indexing sequence TCCGTCTT is bases 13 to 20 of read 1 followed by CG and polyT.|sample name:25Cyclop 05EtOH 24hpf control 4 sc 1972439|scientific name:Danio rerio|strain:mixed", null, null, null, null, null, null, null, null, "Illumina HiSeq 2500 paired end sequencing", "SC EXP 13256 1#13", "10517289", "Illumina sequencing of library 10517289  constructed from sample accession ERS463316 for study accession ERP008536.  This is part of an Illumina multiplexed sequencing run 13256 1.  This submission includes reads tagged with the sequence TCCGTCTT.", "qPCR only", null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "ERP008536", "Illumina HiSeq 2500 paired end sequencing", "ENA FIRST PUBLIC:2014 11 04|ENA LAST UPDATE:2018 11 16", "13256_1#13.cram", "cram", 236289560.0, 1817612.0, "SC RUN 13256 1#13", "0:55 1:75", "A:65497839;C:45569980;G:50614556;T:74585065;N:22120", 55, 75, null, null, 65497839, 45569980, 50614556, 74585065, 22120, "ERX622863", "ERS463316", "ERA375333", "SC", "Wellcome Sanger Institute", 2, 0.16763, 0.7428, 0.08432, 0.16616, 0.95217, 0.83857, 0.73569, 0.69906, 55, 75, "T", "B", "mate1 technical by mapping diff", "illumina", "hiseq_era", "3prime", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United Kingdom", "2014-11-04", "Pharyngula", "Embryo", "Embryo Imprecise", "All anatomical structures"], [16574, "ERR667374", "ERX622862", "ERS463315", "ERP008536", "PRJEB7614", "Screening zebrafish embryos treated with small molecules by transcriptome profiling", "Screening_zebrafish_embryos_treated_with_small_molecules_by_transcriptome_profiling-sc-3143", "Transcriptome Analysis", "Paired end sequence data from the Illumina HiSeq was prepared from RNA of zebrafish embryos treated with small molecules and their corresponding solvent treated and untreated controls.", null, null, null, null, "SAMEA2548469", "SC", "ArrayExpress Species:Danio rerio|ENA FIRST PUBLIC:2014 11 04T16:19:03Z|ENA LAST UPDATE:2018 03 08T17:42:43Z|External Id:SAMEA2548469|INSDC center name:SC|INSDC first public:2014 11 04T16:19:03Z|INSDC last update:2018 03 08T17:42:43Z|INSDC status:public|Submitter Id:25Cyclop 05EtOH 24hpf control 3 sc 1972438|common name:zebrafish|sample description:3 prime end enriched mRNA from single wild type 24hpf embryo treated with 05% v/v ethanol.A 8 base indexing sequence TAAGCGTT is bases 13 to 20 of read 1 followed by CG and polyT.|sample name:25Cyclop 05EtOH 24hpf control 3 sc 1972438|scientific name:Danio rerio|strain:mixed", null, null, null, null, null, null, null, null, "Illumina HiSeq 2500 paired end sequencing", "SC EXP 13256 1#12", "10517288", "Illumina sequencing of library 10517288  constructed from sample accession ERS463315 for study accession ERP008536.  This is part of an Illumina multiplexed sequencing run 13256 1.  This submission includes reads tagged with the sequence TAAGCGTT.", "qPCR only", null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "ERP008536", "Illumina HiSeq 2500 paired end sequencing", "ENA FIRST PUBLIC:2014 11 04|ENA LAST UPDATE:2018 11 16", "13256_1#12.cram", "cram", 381482400.0, 2934480.0, "SC RUN 13256 1#12", "0:55 1:75", "A:108129259;C:71762350;G:76804777;T:124748024;N:37990", 55, 75, null, null, 108129259, 71762350, 76804777, 124748024, 37990, "ERX622862", "ERS463315", "ERA375333", "SC", "Wellcome Sanger Institute", 2, 0.15494, 0.73749, 0.08887, 0.16382, 0.95747, 0.83211, 0.76336, 0.69077, 55, 75, "T", "B", "mate1 technical by mapping diff", "illumina", "hiseq_era", "3prime", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United Kingdom", "2014-11-04", "Pharyngula", "Embryo", "Embryo Imprecise", "All anatomical structures"], [16575, "ERR667373", "ERX622861", "ERS463314", "ERP008536", "PRJEB7614", "Screening zebrafish embryos treated with small molecules by transcriptome profiling", "Screening_zebrafish_embryos_treated_with_small_molecules_by_transcriptome_profiling-sc-3143", "Transcriptome Analysis", "Paired end sequence data from the Illumina HiSeq was prepared from RNA of zebrafish embryos treated with small molecules and their corresponding solvent treated and untreated controls.", null, null, null, null, "SAMEA2548468", "SC", "ArrayExpress Species:Danio rerio|ENA FIRST PUBLIC:2014 11 04T16:19:03Z|ENA LAST UPDATE:2018 03 08T17:41:33Z|External Id:SAMEA2548468|INSDC center name:SC|INSDC first public:2014 11 04T16:19:03Z|INSDC last update:2018 03 08T17:41:33Z|INSDC status:public|Submitter Id:25Cyclop 05EtOH 24hpf control 2 sc 1972437|common name:zebrafish|sample description:3 prime end enriched mRNA from single wild type 24hpf embryo treated with 05% v/v ethanol.A 8 base indexing sequence TCTCGGTT is bases 13 to 20 of read 1 followed by CG and polyT.|sample name:25Cyclop 05EtOH 24hpf control 2 sc 1972437|scientific name:Danio rerio|strain:mixed", null, null, null, null, null, null, null, null, "Illumina HiSeq 2500 paired end sequencing", "SC EXP 13256 1#11", "10517287", "Illumina sequencing of library 10517287  constructed from sample accession ERS463314 for study accession ERP008536.  This is part of an Illumina multiplexed sequencing run 13256 1.  This submission includes reads tagged with the sequence TCTCGGTT.", "qPCR only", null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "ERP008536", "Illumina HiSeq 2500 paired end sequencing", "ENA FIRST PUBLIC:2014 11 04|ENA LAST UPDATE:2018 11 16", "13256_1#11.cram", "cram", 219975210.0, 1692117.0, "SC RUN 13256 1#11", "0:55 1:75", "A:62730862;C:43360964;G:45653286;T:68211390;N:18708", 55, 75, null, null, 62730862, 43360964, 45653286, 68211390, 18708, "ERX622861", "ERS463314", "ERA375333", "SC", "Wellcome Sanger Institute", 2, 0.1335, 0.67379, 0.05402, 0.14367, 0.96319, 0.84877, 0.81344, 0.7263, 55, 75, "T", "B", "mate1 technical by mapping diff", "illumina", "hiseq_era", "3prime", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United Kingdom", "2014-11-04", "Pharyngula", "Embryo", "Embryo Imprecise", "All anatomical structures"], [16576, "ERR667372", "ERX622860", "ERS463313", "ERP008536", "PRJEB7614", "Screening zebrafish embryos treated with small molecules by transcriptome profiling", "Screening_zebrafish_embryos_treated_with_small_molecules_by_transcriptome_profiling-sc-3143", "Transcriptome Analysis", "Paired end sequence data from the Illumina HiSeq was prepared from RNA of zebrafish embryos treated with small molecules and their corresponding solvent treated and untreated controls.", null, null, null, null, "SAMEA2548467", "SC", "ArrayExpress Species:Danio rerio|ENA FIRST PUBLIC:2014 11 04T16:19:03Z|ENA LAST UPDATE:2018 03 08T17:42:05Z|External Id:SAMEA2548467|INSDC center name:SC|INSDC first public:2014 11 04T16:19:03Z|INSDC last update:2018 03 08T17:42:05Z|INSDC status:public|Submitter Id:25Cyclop 05EtOH 24hpf control 1 sc 1972436|common name:zebrafish|sample description:3 prime end enriched mRNA from single wild type 24hpf embryo treated with 05% v/v ethanol.A 8 base indexing sequence TGGTTGTT is bases 13 to 20 of read 1 followed by CG and polyT.|sample name:25Cyclop 05EtOH 24hpf control 1 sc 1972436|scientific name:Danio rerio|strain:mixed", null, null, null, null, null, null, null, null, "Illumina HiSeq 2500 paired end sequencing", "SC EXP 13256 1#10", "10517286", "Illumina sequencing of library 10517286  constructed from sample accession ERS463313 for study accession ERP008536.  This is part of an Illumina multiplexed sequencing run 13256 1.  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This submission includes reads tagged with the sequence GATCAGCG.", "qPCR only", null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "ERP008536", "Illumina HiSeq 2500 paired end sequencing", "ENA FIRST PUBLIC:2014 11 04|ENA LAST UPDATE:2018 11 16", "13256_1#9.cram", "cram", 179568740.0, 1381298.0, "SC RUN 13256 1#9", "0:55 1:75", "A:48952176;C:34402129;G:36985871;T:59212722;N:15842", 55, 75, null, null, 48952176, 34402129, 36985871, 59212722, 15842, "ERX622859", "ERS463312", "ERA375333", "SC", "Wellcome Sanger Institute", 2, 0.16345, 0.77594, 0.10069, 0.16964, 0.95864, 0.83045, 0.78521, 0.7004, 55, 75, "T", "B", "mate1 technical by mapping diff", "illumina", "hiseq_era", "3prime", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United Kingdom", "2014-11-04", "Pharyngula", "Embryo", "Embryo Imprecise", "All anatomical structures"], [16578, "ERR667370", "ERX622858", "ERS463311", "ERP008536", "PRJEB7614", "Screening zebrafish embryos treated with small molecules by transcriptome profiling", "Screening_zebrafish_embryos_treated_with_small_molecules_by_transcriptome_profiling-sc-3143", "Transcriptome Analysis", "Paired end sequence data from the Illumina HiSeq was prepared from RNA of zebrafish embryos treated with small molecules and their corresponding solvent treated and untreated controls.", null, null, null, null, "SAMEA2548465", "SC", "ArrayExpress Species:Danio rerio|ENA FIRST PUBLIC:2014 11 04T16:19:04Z|ENA LAST UPDATE:2018 03 08T17:41:33Z|External Id:SAMEA2548465|INSDC center name:SC|INSDC first public:2014 11 04T16:19:04Z|INSDC last update:2018 03 08T17:41:33Z|INSDC status:public|Submitter Id:25Cyclop 05EtOH 24hpf treated 11 sc 1972434|common name:zebrafish|sample description:3 prime end enriched mRNA from single wild type 24hpf embryo treated with 25 micromolar cyclopamine in 05% v/v ethanol. A 8 base indexing sequence ACTTGATG is bases 13 to 20 of read 1 followed by CG and polyT.|sample name:25Cyclop 05EtOH 24hpf treated 11 sc 1972434|scientific name:Danio rerio|strain:mixed", null, null, null, null, null, null, null, null, "Illumina HiSeq 2500 paired end sequencing", "SC EXP 13256 1#8", "10517284", "Illumina sequencing of library 10517284  constructed from sample accession ERS463311 for study accession ERP008536.  This is part of an Illumina multiplexed sequencing run 13256 1.  This submission includes reads tagged with the sequence ACTTGATG.", "qPCR only", null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "ERP008536", "Illumina HiSeq 2500 paired end sequencing", "ENA FIRST PUBLIC:2014 11 04|ENA LAST UPDATE:2018 11 16", "13256_1#8.cram", "cram", 137020910.0, 1054007.0, "SC RUN 13256 1#8", "0:55 1:75", "A:38049625;C:26414574;G:28139253;T:44406481;N:10977", 55, 75, null, null, 38049625, 26414574, 28139253, 44406481, 10977, "ERX622858", "ERS463311", "ERA375333", "SC", "Wellcome Sanger Institute", 2, 0.1402, 0.74335, 0.08322, 0.1663, 0.96268, 0.84043, 0.77733, 0.71859, 55, 75, "T", "B", "mate1 technical by mapping diff", "illumina", "hiseq_era", "3prime", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United Kingdom", "2014-11-04", "Pharyngula", "Embryo", "Embryo Imprecise", "All anatomical structures"], [16579, "ERR667369", "ERX622857", "ERS463310", "ERP008536", "PRJEB7614", "Screening zebrafish embryos treated with small molecules by transcriptome profiling", "Screening_zebrafish_embryos_treated_with_small_molecules_by_transcriptome_profiling-sc-3143", "Transcriptome Analysis", "Paired end sequence data from the Illumina HiSeq was prepared from RNA of zebrafish embryos treated with small molecules and their corresponding solvent treated and untreated controls.", null, null, null, null, "SAMEA2548464", "SC", "ArrayExpress Species:Danio rerio|ENA FIRST PUBLIC:2014 11 04T16:19:03Z|ENA LAST UPDATE:2018 03 08T17:42:05Z|External Id:SAMEA2548464|INSDC center name:SC|INSDC first public:2014 11 04T16:19:03Z|INSDC last update:2018 03 08T17:42:05Z|INSDC status:public|Submitter Id:25Cyclop 05EtOH 24hpf treated 10 sc 1972433|common name:zebrafish|sample description:3 prime end enriched mRNA from single wild type 24hpf embryo treated with 25 micromolar cyclopamine in 05% v/v ethanol. 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A 8 base indexing sequence GCCAATGT is bases 13 to 20 of read 1 followed by CG and polyT.|sample name:25Cyclop 05EtOH 24hpf treated 9 sc 1972432|scientific name:Danio rerio|strain:mixed", null, null, null, null, null, null, null, null, "Illumina HiSeq 2500 paired end sequencing", "SC EXP 13256 1#6", "10517282", "Illumina sequencing of library 10517282  constructed from sample accession ERS463309 for study accession ERP008536.  This is part of an Illumina multiplexed sequencing run 13256 1.  This submission includes reads tagged with the sequence GCCAATGT.", "qPCR only", null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "ERP008536", "Illumina HiSeq 2500 paired end sequencing", "ENA FIRST PUBLIC:2014 11 04|ENA LAST UPDATE:2018 11 16", "13256_1#6.cram", "cram", 311861420.0, 2398934.0, "SC RUN 13256 1#6", "0:55 1:75", "A:83917355;C:60106233;G:64499135;T:103308448;N:30249", 55, 75, null, null, 83917355, 60106233, 64499135, 103308448, 30249, "ERX622856", "ERS463309", "ERA375333", "SC", "Wellcome Sanger Institute", 2, 0.12297, 0.80619, 0.0593, 0.15961, 0.96234, 0.82248, 0.79929, 0.70162, 55, 75, "T", "B", "mate1 technical by mapping diff", "illumina", "hiseq_era", "3prime", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United Kingdom", "2014-11-04", "Pharyngula", "Embryo", "Embryo Imprecise", "All anatomical structures"], [16581, "ERR667367", "ERX622855", "ERS463308", "ERP008536", "PRJEB7614", "Screening zebrafish embryos treated with small molecules by transcriptome profiling", "Screening_zebrafish_embryos_treated_with_small_molecules_by_transcriptome_profiling-sc-3143", "Transcriptome Analysis", "Paired end sequence data from the Illumina HiSeq was prepared from RNA of zebrafish embryos treated with small molecules and their corresponding solvent treated and untreated controls.", null, null, null, null, "SAMEA2548462", "SC", "ArrayExpress Species:Danio rerio|ENA FIRST PUBLIC:2014 11 04T16:19:03Z|ENA LAST UPDATE:2018 03 08T17:41:33Z|External Id:SAMEA2548462|INSDC center name:SC|INSDC first public:2014 11 04T16:19:03Z|INSDC last update:2018 03 08T17:41:33Z|INSDC status:public|Submitter Id:25Cyclop 05EtOH 24hpf treated 8 sc 1972431|common name:zebrafish|sample description:3 prime end enriched mRNA from single wild type 24hpf embryo treated with 25 micromolar cyclopamine in 05% v/v ethanol. A 8 base indexing sequence ACAGTGGT is bases 13 to 20 of read 1 followed by CG and polyT.|sample name:25Cyclop 05EtOH 24hpf treated 8 sc 1972431|scientific name:Danio rerio|strain:mixed", null, null, null, null, null, null, null, null, "Illumina HiSeq 2500 paired end sequencing", "SC EXP 13256 1#5", "10517281", "Illumina sequencing of library 10517281  constructed from sample accession ERS463308 for study accession ERP008536.  This is part of an Illumina multiplexed sequencing run 13256 1.  This submission includes reads tagged with the sequence ACAGTGGT.", "qPCR only", null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "ERP008536", "Illumina HiSeq 2500 paired end sequencing", "ENA FIRST PUBLIC:2014 11 04|ENA LAST UPDATE:2018 11 16", "13256_1#5.cram", "cram", 319355660.0, 2456582.0, "SC RUN 13256 1#5", "0:55 1:75", "A:84900314;C:64205253;G:67464229;T:102759467;N:26397", 55, 75, null, null, 84900314, 64205253, 67464229, 102759467, 26397, "ERX622855", "ERS463308", "ERA375333", "SC", "Wellcome Sanger Institute", 2, 0.11383, 0.78215, 0.05838, 0.17935, 0.96619, 0.83727, 0.7861, 0.72598, 55, 75, "T", "B", "mate1 technical by mapping diff", "illumina", "hiseq_era", "3prime", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United Kingdom", "2014-11-04", "Pharyngula", "Embryo", "Embryo Imprecise", "All anatomical structures"], [16582, "ERR667366", "ERX622854", "ERS463307", "ERP008536", "PRJEB7614", "Screening zebrafish embryos treated with small molecules by transcriptome profiling", "Screening_zebrafish_embryos_treated_with_small_molecules_by_transcriptome_profiling-sc-3143", "Transcriptome Analysis", "Paired end sequence data from the Illumina HiSeq was prepared from RNA of zebrafish embryos treated with small molecules and their corresponding solvent treated and untreated controls.", null, null, null, null, "SAMEA2548461", "SC", "ArrayExpress Species:Danio rerio|ENA FIRST PUBLIC:2014 11 04T16:19:03Z|ENA LAST UPDATE:2018 03 08T17:42:05Z|External Id:SAMEA2548461|INSDC center name:SC|INSDC first public:2014 11 04T16:19:03Z|INSDC last update:2018 03 08T17:42:05Z|INSDC status:public|Submitter Id:25Cyclop 05EtOH 24hpf treated 7 sc 1972430|common name:zebrafish|sample description:3 prime end enriched mRNA from single wild type 24hpf embryo treated with 25 micromolar cyclopamine in 05% v/v ethanol. A 8 base indexing sequence TGACCACT is bases 13 to 20 of read 1 followed by CG and polyT.|sample name:25Cyclop 05EtOH 24hpf treated 7 sc 1972430|scientific name:Danio rerio|strain:mixed", null, null, null, null, null, null, null, null, "Illumina HiSeq 2500 paired end sequencing", "SC EXP 13256 1#4", "10517280", "Illumina sequencing of library 10517280  constructed from sample accession ERS463307 for study accession ERP008536.  This is part of an Illumina multiplexed sequencing run 13256 1.  This submission includes reads tagged with the sequence TGACCACT.", "qPCR only", null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "ERP008536", "Illumina HiSeq 2500 paired end sequencing", "ENA FIRST PUBLIC:2014 11 04|ENA LAST UPDATE:2018 11 16", "13256_1#4.cram", "cram", 354540160.0, 2727232.0, "SC RUN 13256 1#4", "0:55 1:75", "A:97757414;C:68003475;G:75993436;T:112753315;N:32520", 55, 75, null, null, 97757414, 68003475, 75993436, 112753315, 32520, "ERX622854", "ERS463307", "ERA375333", "SC", "Wellcome Sanger Institute", 2, 0.12969, 0.72947, 0.07042, 0.14757, 0.9614, 0.84017, 0.78018, 0.71871, 55, 75, "T", "B", "mate1 technical by mapping diff", "illumina", "hiseq_era", "3prime", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United Kingdom", "2014-11-04", "Pharyngula", "Embryo", "Embryo Imprecise", "All anatomical structures"], [16583, "ERR667365", "ERX622853", "ERS463306", "ERP008536", "PRJEB7614", "Screening zebrafish embryos treated with small molecules by transcriptome profiling", "Screening_zebrafish_embryos_treated_with_small_molecules_by_transcriptome_profiling-sc-3143", "Transcriptome Analysis", "Paired end sequence data from the Illumina HiSeq was prepared from RNA of zebrafish embryos treated with small molecules and their corresponding solvent treated and untreated controls.", null, null, null, null, "SAMEA2548460", "SC", "ArrayExpress Species:Danio rerio|ENA FIRST PUBLIC:2014 11 04T16:19:03Z|ENA LAST UPDATE:2018 03 08T17:42:43Z|External Id:SAMEA2548460|INSDC center name:SC|INSDC first public:2014 11 04T16:19:03Z|INSDC last update:2018 03 08T17:42:43Z|INSDC status:public|Submitter Id:25Cyclop 05EtOH 24hpf treated 6 sc 1972429|common name:zebrafish|sample description:3 prime end enriched mRNA from single wild type 24hpf embryo treated with 25 micromolar cyclopamine in 05% v/v ethanol. A 8 base indexing sequence TTAGGCAT is bases 13 to 20 of read 1 followed by CG and polyT.|sample name:25Cyclop 05EtOH 24hpf treated 6 sc 1972429|scientific name:Danio rerio|strain:mixed", null, null, null, null, null, null, null, null, "Illumina HiSeq 2500 paired end sequencing", "SC EXP 13256 1#3", "10517279", "Illumina sequencing of library 10517279  constructed from sample accession ERS463306 for study accession ERP008536.  This is part of an Illumina multiplexed sequencing run 13256 1.  This submission includes reads tagged with the sequence TTAGGCAT.", "qPCR only", null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "ERP008536", "Illumina HiSeq 2500 paired end sequencing", "ENA FIRST PUBLIC:2014 11 04|ENA LAST UPDATE:2018 11 16", "13256_1#3.cram", "cram", 602820270.0, 4637079.0, "SC RUN 13256 1#3", "0:55 1:75", "A:165474925;C:115590273;G:123825630;T:197870518;N:58924", 55, 75, null, null, 165474925, 115590273, 123825630, 197870518, 58924, "ERX622853", "ERS463306", "ERA375333", "SC", "Wellcome Sanger Institute", 2, 0.141, 0.74407, 0.08834, 0.15443, 0.96475, 0.83958, 0.78331, 0.7194, 55, 75, "T", "B", "mate1 technical by mapping diff", "illumina", "hiseq_era", "3prime", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United Kingdom", "2014-11-04", "Pharyngula", "Embryo", "Embryo Imprecise", "All anatomical structures"], [16584, "ERR667364", "ERX622852", "ERS463305", "ERP008536", "PRJEB7614", "Screening zebrafish embryos treated with small molecules by transcriptome profiling", "Screening_zebrafish_embryos_treated_with_small_molecules_by_transcriptome_profiling-sc-3143", "Transcriptome Analysis", "Paired end sequence data from the Illumina HiSeq was prepared from RNA of zebrafish embryos treated with small molecules and their corresponding solvent treated and untreated controls.", null, null, null, null, "SAMEA2548459", "SC", "ArrayExpress Species:Danio rerio|ENA FIRST PUBLIC:2014 11 04T16:19:03Z|ENA LAST UPDATE:2018 03 08T17:41:33Z|External Id:SAMEA2548459|INSDC center name:SC|INSDC first public:2014 11 04T16:19:03Z|INSDC last update:2018 03 08T17:41:33Z|INSDC status:public|Submitter Id:25Cyclop 05EtOH 24hpf treated 4 sc 1972428|common name:zebrafish|sample description:3 prime end enriched mRNA from single wild type 24hpf embryo treated with 25 micromolar cyclopamine in 05% v/v ethanol. 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This submission includes reads tagged with the sequence CGATGTTT.", "qPCR only", null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "ERP008536", "Illumina HiSeq 2500 paired end sequencing", "ENA FIRST PUBLIC:2014 11 04|ENA LAST UPDATE:2018 11 16", "13256_1#2.cram", "cram", 192840440.0, 1483388.0, "SC RUN 13256 1#2", "0:55 1:75", "A:53469380;C:37747264;G:40238127;T:61365974;N:19695", 55, 75, null, null, 53469380, 37747264, 40238127, 61365974, 19695, "ERX622852", "ERS463305", "ERA375333", "SC", "Wellcome Sanger Institute", 2, 0.14229, 0.74015, 0.07887, 0.16243, 0.96357, 0.84687, 0.7426, 0.72806, 55, 75, "T", "B", "mate1 technical by mapping diff", "illumina", "hiseq_era", "3prime", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United Kingdom", "2014-11-04", "Pharyngula", "Embryo", "Embryo Imprecise", "All anatomical structures"]], "truncated": false, "filtered_table_rows_count": 5278, "expanded_columns": [], 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