{"database": "metadata", "table": "run_metadata", "is_view": false, "human_description_en": "where experiment.library_layout = \"PAIRED\", experiment.library_selection = \"unspecified\" and technology = \"10x\"", "rows": [[25302, "SRR25793493", "SRX21515745", "SRS18742910", "SRP457465", "PRJNA1010662", "The miR 144/Hmgn2 regulatory axis orchestrates chromatin organization during erythropoiesis.", "PRJNA1010662", "Other", "Differentiation of stem and progenitor cells is a highly regulated process that involves the coordinated action of multiple layers of regulation. Here we show how the post transcriptional regulatory layer instructs the chromatin regulation level via miR 144 and its targets to orchestrate chromatin condensation during erythropoiesis. The loss of miR 144 leads to impaired chromatin condensation during erythrocyte maturation.", null, null, null, "Single cell Pr1phros ADULT miR 144 mutant Danio rerio", "miR 144", null, "isolate:miR 144 mutant|age:Adult|collection date:N/A|geo loc name:N/A|sex:mixed|tissue:blood|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Single cell Pr1phros ADULT miR 144 mutant Danio rerio", "CD 144 1", "CD 144 1", "Single cell library cloning was done with 10X Genomics", null, null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "unspecified", "PAIRED", "ILLUMINA", "NextSeq 2000", null, "SRP457465", null, null, "CD_144_1_S3_R1.fastq CD_144_1_S3_R2.fastq", "fastq fastq", 12899093774.0, 108395746.0, "CD 144 1 S3 R1.fastq", "0:28 1:91", "A:3225260764;C:3211986177;G:3151471558;T:3308055659;N:2319616", 28, 91, null, null, 3225260764, 3211986177, 3151471558, 3308055659, 2319616, "SRX21515745", "SRS18742910", "SRA1701829", "University of East Anglia|Biological Sciences", "University of East Anglia", 2, 0.00483, 0.97382, 0.00157, 0.05913, 0.99439, 0.86815, 0.46634, 0.45907, 28, 91, "T", "B", "sc-like readlen", "illumina", "nextseq_v2", "unknown", "unknown", "unknown", "sc", "single_cell_droplet", "10x", null, "United Kingdom", "2023-08-30", "Adult", "Adult", "Blood", "Hematopoietic System"], [25303, "SRR25793494", "SRX21515744", "SRS18742909", "SRP457465", "PRJNA1010662", "The miR 144/Hmgn2 regulatory axis orchestrates chromatin organization during erythropoiesis.", "PRJNA1010662", "Other", "Differentiation of stem and progenitor cells is a highly regulated process that involves the coordinated action of multiple layers of regulation. Here we show how the post transcriptional regulatory layer instructs the chromatin regulation level via miR 144 and its targets to orchestrate chromatin condensation during erythropoiesis. The loss of miR 144 leads to impaired chromatin condensation during erythrocyte maturation.", null, null, null, "Single cell Pr1phros ADULT Wild type Danio rerio", "WT", null, "isolate:Wildtype|age:Adult|collection date:N/A|geo loc name:N/A|sex:mixed|tissue:blood|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Single cell Pr1phros ADULT Wild type Danio rerio", "CD wt 1", "CD wt 1", "Single cell library cloning was done with 10X Genomics", null, null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "unspecified", "PAIRED", "ILLUMINA", "NextSeq 2000", null, "SRP457465", null, null, "CD_wt_1_S2_R1.fastq CD_wt_1_S2_R2.fastq", "fastq fastq", 12715533299.0, 106853221.0, "CD wt 1 S2 R1.fastq", "0:28 1:91", "A:3324705878;C:3042843364;G:3105568183;T:3240105528;N:2310346", 28, 91, null, null, 3324705878, 3042843364, 3105568183, 3240105528, 2310346, "SRX21515744", "SRS18742909", "SRA1701829", "University of East Anglia|Biological Sciences", "University of East Anglia", 2, 0.00593, 0.93177, 0.00191, 0.09596, 0.99257, 0.84394, 0.42447, 0.4869, 28, 91, "T", "B", "sc-like readlen", "illumina", "nextseq_v2", "unknown", "unknown", "unknown", "sc", "single_cell_droplet", "10x", null, "United Kingdom", "2023-08-30", "Adult", "Adult", "Blood", "Hematopoietic System"], [74615, "SRR24037111", "SRX19839674", "SRS17198396", "SRP427499", "PRJNA945049", "linc mipep and linc wrb encode micropeptides that regulate chromatin accessibility in vertebrate specific neural cells", "PRJNA945049", "Other", "Thousands of long intergenic non coding RNAs lincRNAs are transcribed throughout the vertebrate genome. A subset of lincRNAs enriched in developing brains have recently been found to contain cryptic open reading frames and are speculated to encode micropeptides. However  systematic identification and functional assessment of these transcripts have been hindered by technical challenges caused by their small size. Here we show that two putative lincRNAs linc mipep  also called lnc rps25  and linc wrb encode micropeptides with homology to the vertebrate specific chromatin architectural protein  Hmgn1  and demonstrate that they are required for development of vertebrate specific brain cell types. Specifically  we show that NMDA receptor mediated pathways are dysregulated in zebrafish lacking these micropeptides and that their loss preferentially alters the gene regulatory networks that establish cerebellar cells and oligodendrocytes   evolutionarily newer cell types that develop postnatally in humans. 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A subset of lincRNAs enriched in developing brains have recently been found to contain cryptic open reading frames and are speculated to encode micropeptides. However  systematic identification and functional assessment of these transcripts have been hindered by technical challenges caused by their small size. Here we show that two putative lincRNAs linc mipep  also called lnc rps25  and linc wrb encode micropeptides with homology to the vertebrate specific chromatin architectural protein  Hmgn1  and demonstrate that they are required for development of vertebrate specific brain cell types. Specifically  we show that NMDA receptor mediated pathways are dysregulated in zebrafish lacking these micropeptides and that their loss preferentially alters the gene regulatory networks that establish cerebellar cells and oligodendrocytes   evolutionarily newer cell types that develop postnatally in humans. These findings reveal a key missing link in the evolution of vertebrate brain cell development and illustrate a genetic basis for how some neural cell types are more susceptible to chromatin disruptions  with implications for neurodevelopmental disorders and disease.", null, null, null, "linc mipep scMultiome 6d brains   linc mipep mutant scRNA seq [10x BAM]", "linc mipep scMultiome 6d brains   linc mipepMut scRNA AGN002629 [10x BAM]", null, "strain:TU/AB|age:144|dev stage:Day 6|sex:pooled male and female|tissue:brain|genotype: / |strain maternal:linc mipep MUT|strain paternal:linc mipep MUT|molecule:RNA|sample ref:AGS002016|replicate ref:AGN002629|replicate order:1|project label long:linc mipep homozygous mutant vs WT siblings sc Multiome 6dpf brains|project label short:linc mipep scMultiome 6d brains|sample label short:Mut RNA|replicate label short:linc mipepMut scRNA|filetype:bam|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "linc mipep 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