{"database": "metadata", "table": "run_metadata", "is_view": false, "human_description_en": "where experiment.library_layout = \"PAIRED\", experiment.library_selection = \"PolyA\" and tissue_curation = \"Brain\"", "rows": [[11711, "ERR11282347", "ERX10689990", "ERS15395535", "ERP146895", "PRJEB61813", "RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month", "E-MTAB-12901", "Other", "The ATP binding cassette subfamily B member 1 ABCB1  encoding a multidrug transporter P glycoprotein  plays a critical role in the efflux of xenobiotics in humans and is implicated in cancer resistance to chemotherapy\u2014however  little information regarding Pgp at the zebrafish. In addition  to study the function of Pgp in the zebrafish brain in the aging process  we performed RNA seq using brain tissue of WT and abcb4 knockout zebrafish at different ages  such as 2 month and 30 month.", "ENA FIRST PUBLIC:2023 07 14|ENA LAST UPDATE:2023 07 14", null, "Protocols: brain tissue were dissected from zebrafish and snap frozen until RNA isolation Trizol + zymo RNA clean column kit TruSeq RNA sample prep kit Illumina to prepare cDNA libraries post PolyA selection", "Sample 8", "SAMEA113400389", "VANDERBILT UNIVERSITY", "ENA FIRST PUBLIC:2023 07 14T00:23:41Z|ENA LAST UPDATE:2023 07 14T00:23:41Z|External Id:SAMEA113400389|INSDC center alias:VANDERBILT UNIVERSITY|INSDC center name:VANDERBILT UNIVERSITY|INSDC first public:2023 07 14T00:23:41Z|INSDC last update:2023 07 14T00:23:41Z|INSDC status:public|Submitter Id:E MTAB 12901:Sample 8|age:2|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|disease:normal|genotype:wild type genotype|organism part:brain|sample name:E MTAB 12901:Sample 8|scientific name:Danio rerio|sex:female|strain:AB", null, null, null, null, null, null, null, null, "Illumina NovaSeq 6000 paired end sequencing; RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month", "E MTAB 12901:Sample 8 p", "Sample 8 p", "RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month", "brain tissue were dissected from zebrafish and snap frozen until RNA isolation Trizol + zymo RNA clean column kit TruSeq RNA sample prep kit Illumina to prepare cDNA libraries post PolyA selection", "Experimental Factor: genotype:wild type genotype", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP146895", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month", "ENA FIRST PUBLIC:2023 07 14|ENA LAST UPDATE:2023 07 14", "8680-JP-0008_S1_L005_R2_001.fastq.gz 8680-JP-0008_S1_L005_R1_001.fastq.gz", "fastq fastq", 21919844332.0, 72582266.0, "E MTAB 12901:8680 JP 0008 S1 L005 R", "0:151 1:151", "A:5996325335;C:4770833694;G:5244802952;T:5876120950;N:31761401", 151, 151, null, null, 5996325335, 4770833694, 5244802952, 5876120950, 31761401, "ERX10689990", "ERS15395535", "ERA23235100", "VANDERBILT UNIVERSITY|European Nucleotide Archive", "VANDERBILT UNIVERSITY|European Nucleotide Archive", 2, 0.92572, 0.92425, 0.13427, 0.13101, 0.70086, 0.70266, 0.50868, 0.50381, 151, 151, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "United States", "2023-07-14", "Adult", "Adult", "Brain", "Nervous System"], [11712, "ERR11282346", "ERX10689989", "ERS15395534", "ERP146895", "PRJEB61813", "RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month", "E-MTAB-12901", "Other", "The ATP binding cassette subfamily B member 1 ABCB1  encoding a multidrug transporter P glycoprotein  plays a critical role in the efflux of xenobiotics in humans and is implicated in cancer resistance to chemotherapy\u2014however  little information regarding Pgp at the zebrafish. In addition  to study the function of Pgp in the zebrafish brain in the aging process  we performed RNA seq using brain tissue of WT and abcb4 knockout zebrafish at different ages  such as 2 month and 30 month.", "ENA FIRST PUBLIC:2023 07 14|ENA LAST UPDATE:2023 07 14", null, "Protocols: brain tissue were dissected from zebrafish and snap frozen until RNA isolation Trizol + zymo RNA clean column kit TruSeq RNA sample prep kit Illumina to prepare cDNA libraries post PolyA selection", "Sample 7", "SAMEA113400388", "VANDERBILT UNIVERSITY", "ENA FIRST PUBLIC:2023 07 14T00:23:41Z|ENA LAST UPDATE:2023 07 14T00:23:41Z|External Id:SAMEA113400388|INSDC center alias:VANDERBILT UNIVERSITY|INSDC center name:VANDERBILT UNIVERSITY|INSDC first public:2023 07 14T00:23:41Z|INSDC last update:2023 07 14T00:23:41Z|INSDC status:public|Submitter Id:E MTAB 12901:Sample 7|age:2|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|disease:normal|genotype:wild type genotype|organism part:brain|sample name:E MTAB 12901:Sample 7|scientific name:Danio rerio|sex:female|strain:AB", null, null, null, null, null, null, null, null, "Illumina NovaSeq 6000 paired end sequencing; RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month", "E MTAB 12901:Sample 7 p", "Sample 7 p", "RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month", "brain tissue were dissected from zebrafish and snap frozen until RNA isolation Trizol + zymo RNA clean column kit TruSeq RNA sample prep kit Illumina to prepare cDNA libraries post PolyA selection", "Experimental Factor: genotype:wild type genotype", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP146895", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month", "ENA FIRST PUBLIC:2023 07 14|ENA LAST UPDATE:2023 07 14", "8680-JP-0007_S1_L005_R2_001.fastq.gz 8680-JP-0007_S1_L005_R1_001.fastq.gz", "fastq fastq", 20351465316.0, 67388958.0, "E MTAB 12901:8680 JP 0007 S1 L005 R", "0:151 1:151", "A:5563989836;C:4461170993;G:4892290448;T:5404916249;N:29097790", 151, 151, null, null, 5563989836, 4461170993, 4892290448, 5404916249, 29097790, "ERX10689989", "ERS15395534", "ERA23235100", "VANDERBILT UNIVERSITY|European Nucleotide Archive", "VANDERBILT UNIVERSITY|European Nucleotide Archive", 2, 0.9267, 0.9236, 0.12782, 0.12528, 0.70307, 0.70473, 0.50799, 0.50969, 151, 151, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "United States", "2023-07-14", "Adult", "Adult", "Brain", "Nervous System"], [11713, "ERR11282345", "ERX10689988", "ERS15395533", "ERP146895", "PRJEB61813", "RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month", "E-MTAB-12901", "Other", "The ATP binding cassette subfamily B member 1 ABCB1  encoding a multidrug transporter P glycoprotein  plays a critical role in the efflux of xenobiotics in humans and is implicated in cancer resistance to chemotherapy\u2014however  little information regarding Pgp at the zebrafish. In addition  to study the function of Pgp in the zebrafish brain in the aging process  we performed RNA seq using brain tissue of WT and abcb4 knockout zebrafish at different ages  such as 2 month and 30 month.", "ENA FIRST PUBLIC:2023 07 14|ENA LAST UPDATE:2023 07 14", null, "Protocols: brain tissue were dissected from zebrafish and snap frozen until RNA isolation Trizol + zymo RNA clean column kit TruSeq RNA sample prep kit Illumina to prepare cDNA libraries post PolyA selection", "Sample 6", "SAMEA113400387", "VANDERBILT UNIVERSITY", "ENA FIRST PUBLIC:2023 07 14T00:23:41Z|ENA LAST UPDATE:2023 07 14T00:23:41Z|External Id:SAMEA113400387|INSDC center alias:VANDERBILT UNIVERSITY|INSDC center name:VANDERBILT UNIVERSITY|INSDC first public:2023 07 14T00:23:41Z|INSDC last update:2023 07 14T00:23:41Z|INSDC status:public|Submitter Id:E MTAB 12901:Sample 6|age:2|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|disease:normal|genotype:wild type genotype|organism part:brain|sample name:E MTAB 12901:Sample 6|scientific name:Danio rerio|sex:female|strain:AB", null, null, null, null, null, null, null, null, "Illumina NovaSeq 6000 paired end sequencing; RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month", "E MTAB 12901:Sample 6 p", "Sample 6 p", "RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month", "brain tissue were dissected from zebrafish and snap frozen until RNA isolation Trizol + zymo RNA clean column kit TruSeq RNA sample prep kit Illumina to prepare cDNA libraries post PolyA selection", "Experimental Factor: genotype:wild type genotype", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP146895", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month", "ENA FIRST PUBLIC:2023 07 14|ENA LAST UPDATE:2023 07 14", "8680-JP-0006_S1_L005_R2_001.fastq.gz 8680-JP-0006_S1_L005_R1_001.fastq.gz", "fastq fastq", 19220102648.0, 63642724.0, "E MTAB 12901:8680 JP 0006 S1 L005 R", "0:151 1:151", "A:5250476003;C:4214339559;G:4606611173;T:5120701154;N:27974759", 151, 151, null, null, 5250476003, 4214339559, 4606611173, 5120701154, 27974759, "ERX10689988", "ERS15395533", "ERA23235100", "VANDERBILT UNIVERSITY|European Nucleotide Archive", "VANDERBILT UNIVERSITY|European Nucleotide Archive", 2, 0.92441, 0.92391, 0.13718, 0.13346, 0.70281, 0.7024, 0.48155, 0.49173, 151, 151, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "United States", "2023-07-14", "Adult", "Adult", "Brain", "Nervous System"], [11714, "ERR11282344", "ERX10689987", "ERS15395532", "ERP146895", "PRJEB61813", "RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month", "E-MTAB-12901", "Other", "The ATP binding cassette subfamily B member 1 ABCB1  encoding a multidrug transporter P glycoprotein  plays a critical role in the efflux of xenobiotics in humans and is implicated in cancer resistance to chemotherapy\u2014however  little information regarding Pgp at the zebrafish. In addition  to study the function of Pgp in the zebrafish brain in the aging process  we performed RNA seq using brain tissue of WT and abcb4 knockout zebrafish at different ages  such as 2 month and 30 month.", "ENA FIRST PUBLIC:2023 07 14|ENA LAST UPDATE:2023 07 14", null, "Protocols: brain tissue were dissected from zebrafish and snap frozen until RNA isolation Trizol + zymo RNA clean column kit TruSeq RNA sample prep kit Illumina to prepare cDNA libraries post PolyA selection", "Sample 3", "SAMEA113400386", "VANDERBILT UNIVERSITY", "ENA FIRST PUBLIC:2023 07 14T00:23:41Z|ENA LAST UPDATE:2023 07 14T00:23:41Z|External Id:SAMEA113400386|INSDC center alias:VANDERBILT UNIVERSITY|INSDC center name:VANDERBILT UNIVERSITY|INSDC first public:2023 07 14T00:23:41Z|INSDC last update:2023 07 14T00:23:41Z|INSDC status:public|Submitter Id:E MTAB 12901:Sample 3|age:30|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|disease:normal|genotype:wild type genotype|organism part:brain|sample name:E MTAB 12901:Sample 3|scientific name:Danio rerio|sex:female|strain:AB", null, null, null, null, null, null, null, null, "Illumina NovaSeq 6000 paired end sequencing; RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month", "E MTAB 12901:Sample 3 p", "Sample 3 p", "RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month", "brain tissue were dissected from zebrafish and snap frozen until RNA isolation Trizol + zymo RNA clean column kit TruSeq RNA sample prep kit Illumina to prepare cDNA libraries post PolyA selection", "Experimental Factor: genotype:wild type genotype", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP146895", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month", "ENA FIRST PUBLIC:2023 07 14|ENA LAST UPDATE:2023 07 14", "8680-JP-0003_S1_L005_R1_001.fastq.gz 8680-JP-0003_S1_L005_R2_001.fastq.gz", "fastq fastq", 18624364462.0, 61670081.0, "E MTAB 12901:8680 JP 0003 S1 L005 R", "0:151 1:151", "A:5114421711;C:4075705896;G:4436341056;T:4970953410;N:26942389", 151, 151, null, null, 5114421711, 4075705896, 4436341056, 4970953410, 26942389, "ERX10689987", "ERS15395532", "ERA23235100", "VANDERBILT UNIVERSITY|European Nucleotide Archive", "VANDERBILT UNIVERSITY|European Nucleotide Archive", 2, 0.92553, 0.92378, 0.13069, 0.12709, 0.69988, 0.70258, 0.4969, 0.51157, 151, 151, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "United States", "2023-07-14", "Adult", "Adult", "Brain", "Nervous System"], [11715, "ERR11282343", "ERX10689986", "ERS15395531", "ERP146895", "PRJEB61813", "RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month", "E-MTAB-12901", "Other", "The ATP binding cassette subfamily B member 1 ABCB1  encoding a multidrug transporter P glycoprotein  plays a critical role in the efflux of xenobiotics in humans and is implicated in cancer resistance to chemotherapy\u2014however  little information regarding Pgp at the zebrafish. In addition  to study the function of Pgp in the zebrafish brain in the aging process  we performed RNA seq using brain tissue of WT and abcb4 knockout zebrafish at different ages  such as 2 month and 30 month.", "ENA FIRST PUBLIC:2023 07 14|ENA LAST UPDATE:2023 07 14", null, "Protocols: brain tissue were dissected from zebrafish and snap frozen until RNA isolation Trizol + zymo RNA clean column kit TruSeq RNA sample prep kit Illumina to prepare cDNA libraries post PolyA selection", "Sample 2", "SAMEA113400385", "VANDERBILT UNIVERSITY", "ENA FIRST PUBLIC:2023 07 14T00:23:41Z|ENA LAST UPDATE:2023 07 14T00:23:41Z|External Id:SAMEA113400385|INSDC center alias:VANDERBILT UNIVERSITY|INSDC center name:VANDERBILT UNIVERSITY|INSDC first public:2023 07 14T00:23:41Z|INSDC last update:2023 07 14T00:23:41Z|INSDC status:public|Submitter Id:E MTAB 12901:Sample 2|age:30|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|disease:normal|genotype:wild type genotype|organism part:brain|sample name:E MTAB 12901:Sample 2|scientific name:Danio rerio|sex:female|strain:AB", null, null, null, null, null, null, null, null, "Illumina NovaSeq 6000 paired end sequencing; RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month", "E MTAB 12901:Sample 2 p", "Sample 2 p", "RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month", "brain tissue were dissected from zebrafish and snap frozen until RNA isolation Trizol + zymo RNA clean column kit TruSeq RNA sample prep kit Illumina to prepare cDNA libraries post PolyA selection", "Experimental Factor: genotype:wild type genotype", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP146895", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month", "ENA FIRST PUBLIC:2023 07 14|ENA LAST UPDATE:2023 07 14", "8680-JP-0002_S1_L005_R1_001.fastq.gz 8680-JP-0002_S1_L005_R2_001.fastq.gz", "fastq fastq", 15153807038.0, 50178169.0, "E MTAB 12901:8680 JP 0002 S1 L005 R", "0:151 1:151", "A:4156183785;C:3309476575;G:3635942314;T:4030261947;N:21942417", 151, 151, null, null, 4156183785, 3309476575, 3635942314, 4030261947, 21942417, "ERX10689986", "ERS15395531", "ERA23235100", "VANDERBILT UNIVERSITY|European Nucleotide Archive", "VANDERBILT UNIVERSITY|European Nucleotide Archive", 2, 0.92069, 0.91964, 0.13733, 0.13466, 0.70102, 0.70185, 0.48507, 0.48992, 151, 151, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "United States", "2023-07-14", "Adult", "Adult", "Brain", "Nervous System"], [11716, "ERR11282342", "ERX10689985", "ERS15395530", "ERP146895", "PRJEB61813", "RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month", "E-MTAB-12901", "Other", "The ATP binding cassette subfamily B member 1 ABCB1  encoding a multidrug transporter P glycoprotein  plays a critical role in the efflux of xenobiotics in humans and is implicated in cancer resistance to chemotherapy\u2014however  little information regarding Pgp at the zebrafish. In addition  to study the function of Pgp in the zebrafish brain in the aging process  we performed RNA seq using brain tissue of WT and abcb4 knockout zebrafish at different ages  such as 2 month and 30 month.", "ENA FIRST PUBLIC:2023 07 14|ENA LAST UPDATE:2023 07 14", null, "Protocols: brain tissue were dissected from zebrafish and snap frozen until RNA isolation Trizol + zymo RNA clean column kit TruSeq RNA sample prep kit Illumina to prepare cDNA libraries post PolyA selection", "Sample 1", "SAMEA113400384", "VANDERBILT UNIVERSITY", "ENA FIRST PUBLIC:2023 07 14T00:23:41Z|ENA LAST UPDATE:2023 07 14T00:23:41Z|External Id:SAMEA113400384|INSDC center alias:VANDERBILT UNIVERSITY|INSDC center name:VANDERBILT UNIVERSITY|INSDC first public:2023 07 14T00:23:41Z|INSDC last update:2023 07 14T00:23:41Z|INSDC status:public|Submitter Id:E MTAB 12901:Sample 1|age:30|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|disease:normal|genotype:wild type genotype|organism part:brain|sample name:E MTAB 12901:Sample 1|scientific name:Danio rerio|sex:female|strain:AB", null, null, null, null, null, null, null, null, "Illumina NovaSeq 6000 paired end sequencing; RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month", "E MTAB 12901:Sample 1 p", "Sample 1 p", "RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month", "brain tissue were dissected from zebrafish and snap frozen until RNA isolation Trizol + zymo RNA clean column kit TruSeq RNA sample prep kit Illumina to prepare cDNA libraries post PolyA selection", "Experimental Factor: genotype:wild type genotype", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP146895", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month", "ENA FIRST PUBLIC:2023 07 14|ENA LAST UPDATE:2023 07 14", "8680-JP-0001_S1_L005_R2_001.fastq.gz 8680-JP-0001_S1_L005_R1_001.fastq.gz", "fastq fastq", 17758550696.0, 58803148.0, "E MTAB 12901:8680 JP 0001 S1 L005 R", "0:151 1:151", "A:4866917250;C:3906782394;G:4241519777;T:4717566830;N:25764445", 151, 151, null, null, 4866917250, 3906782394, 4241519777, 4717566830, 25764445, "ERX10689985", "ERS15395530", "ERA23235100", "VANDERBILT UNIVERSITY|European Nucleotide Archive", "VANDERBILT UNIVERSITY|European Nucleotide Archive", 2, 0.92263, 0.92154, 0.13341, 0.12969, 0.69443, 0.69658, 0.48648, 0.49678, 151, 151, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "United States", "2023-07-14", "Adult", "Adult", "Brain", "Nervous System"], [11717, "ERR11282341", "ERX10689984", "ERS15395529", "ERP146895", "PRJEB61813", "RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month", "E-MTAB-12901", "Other", "The ATP binding cassette subfamily B member 1 ABCB1  encoding a multidrug transporter P glycoprotein  plays a critical role in the efflux of xenobiotics in humans and is implicated in cancer resistance to chemotherapy\u2014however  little information regarding Pgp at the zebrafish. In addition  to study the function of Pgp in the zebrafish brain in the aging process  we performed RNA seq using brain tissue of WT and abcb4 knockout zebrafish at different ages  such as 2 month and 30 month.", "ENA FIRST PUBLIC:2023 07 14|ENA LAST UPDATE:2023 07 14", null, "Protocols: brain tissue were dissected from zebrafish and snap frozen until RNA isolation Trizol + zymo RNA clean column kit TruSeq RNA sample prep kit Illumina to prepare cDNA libraries post PolyA selection", "Sample 9", "SAMEA113400383", "VANDERBILT UNIVERSITY", "ENA FIRST PUBLIC:2023 07 14T00:23:41Z|ENA LAST UPDATE:2023 07 14T00:23:41Z|External Id:SAMEA113400383|INSDC center alias:VANDERBILT UNIVERSITY|INSDC center name:VANDERBILT UNIVERSITY|INSDC first public:2023 07 14T00:23:41Z|INSDC last update:2023 07 14T00:23:41Z|INSDC status:public|Submitter Id:E MTAB 12901:Sample 9|age:2|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|disease:normal|genotype:abcb4  / |organism part:brain|sample name:E MTAB 12901:Sample 9|scientific name:Danio rerio|sex:female|strain:AB", null, null, null, null, null, null, null, null, "Illumina NovaSeq 6000 paired end sequencing; RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month", "E MTAB 12901:Sample 9 p", "Sample 9 p", "RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month", "brain tissue were dissected from zebrafish and snap frozen until RNA isolation Trizol + zymo RNA clean column kit TruSeq RNA sample prep kit Illumina to prepare cDNA libraries post PolyA selection", "Experimental Factor: genotype:abcb4  / ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP146895", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month", "ENA FIRST PUBLIC:2023 07 14|ENA LAST UPDATE:2023 07 14", "8680-JP-0009_S1_L005_R2_001.fastq.gz 8680-JP-0009_S1_L005_R1_001.fastq.gz", "fastq fastq", 21619735456.0, 71588528.0, "E MTAB 12901:8680 JP 0009 S1 L005 R", "0:151 1:151", "A:5904040180;C:4720941204;G:5229712921;T:5733559571;N:31481580", 151, 151, null, null, 5904040180, 4720941204, 5229712921, 5733559571, 31481580, "ERX10689984", "ERS15395529", "ERA23235100", "VANDERBILT UNIVERSITY|European Nucleotide Archive", "VANDERBILT UNIVERSITY|European Nucleotide Archive", 2, 0.92235, 0.92126, 0.13956, 0.13682, 0.70378, 0.70526, 0.4929, 0.49286, 151, 151, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "United States", "2023-07-14", "Adult", "Adult", "Brain", "Nervous System"], [11718, "ERR11282340", "ERX10689983", "ERS15395528", "ERP146895", "PRJEB61813", "RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month", "E-MTAB-12901", "Other", "The ATP binding cassette subfamily B member 1 ABCB1  encoding a multidrug transporter P glycoprotein  plays a critical role in the efflux of xenobiotics in humans and is implicated in cancer resistance to chemotherapy\u2014however  little information regarding Pgp at the zebrafish. In addition  to study the function of Pgp in the zebrafish brain in the aging process  we performed RNA seq using brain tissue of WT and abcb4 knockout zebrafish at different ages  such as 2 month and 30 month.", "ENA FIRST PUBLIC:2023 07 14|ENA LAST UPDATE:2023 07 14", null, "Protocols: brain tissue were dissected from zebrafish and snap frozen until RNA isolation Trizol + zymo RNA clean column kit TruSeq RNA sample prep kit Illumina to prepare cDNA libraries post PolyA selection", "Sample 11", "SAMEA113400382", "VANDERBILT UNIVERSITY", "ENA FIRST PUBLIC:2023 07 14T00:23:41Z|ENA LAST UPDATE:2023 07 14T00:23:41Z|External Id:SAMEA113400382|INSDC center alias:VANDERBILT UNIVERSITY|INSDC center name:VANDERBILT UNIVERSITY|INSDC first public:2023 07 14T00:23:41Z|INSDC last update:2023 07 14T00:23:41Z|INSDC status:public|Submitter Id:E MTAB 12901:Sample 11|age:2|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|disease:normal|genotype:abcb4  / |organism part:brain|sample name:E MTAB 12901:Sample 11|scientific name:Danio rerio|sex:female|strain:AB", null, null, null, null, null, null, null, null, "Illumina NovaSeq 6000 paired end sequencing; RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month", "E MTAB 12901:Sample 11 p", "Sample 11 p", "RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month", "brain tissue were dissected from zebrafish and snap frozen until RNA isolation Trizol + zymo RNA clean column kit TruSeq RNA sample prep kit Illumina to prepare cDNA libraries post PolyA selection", "Experimental Factor: genotype:abcb4  / ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP146895", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month", "ENA FIRST PUBLIC:2023 07 14|ENA LAST UPDATE:2023 07 14", "8680-JP-0011_S1_L005_R1_001.fastq.gz 8680-JP-0011_S1_L005_R2_001.fastq.gz", "fastq fastq", 20372991574.0, 67460237.0, "E MTAB 12901:8680 JP 0011 S1 L005 R", "0:151 1:151", "A:5569250549;C:4448986210;G:4925237941;T:5399892024;N:29624850", 151, 151, null, null, 5569250549, 4448986210, 4925237941, 5399892024, 29624850, "ERX10689983", "ERS15395528", "ERA23235100", "VANDERBILT UNIVERSITY|European Nucleotide Archive", "VANDERBILT UNIVERSITY|European Nucleotide Archive", 2, 0.92578, 0.92449, 0.12979, 0.12632, 0.69666, 0.69775, 0.51379, 0.51557, 151, 151, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "United States", "2023-07-14", "Adult", "Adult", "Brain", "Nervous System"], [11719, "ERR11282339", "ERX10689982", "ERS15395527", "ERP146895", "PRJEB61813", "RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month", "E-MTAB-12901", "Other", "The ATP binding cassette subfamily B member 1 ABCB1  encoding a multidrug transporter P glycoprotein  plays a critical role in the efflux of xenobiotics in humans and is implicated in cancer resistance to chemotherapy\u2014however  little information regarding Pgp at the zebrafish. In addition  to study the function of Pgp in the zebrafish brain in the aging process  we performed RNA seq using brain tissue of WT and abcb4 knockout zebrafish at different ages  such as 2 month and 30 month.", "ENA FIRST PUBLIC:2023 07 14|ENA LAST UPDATE:2023 07 14", null, "Protocols: brain tissue were dissected from zebrafish and snap frozen until RNA isolation Trizol + zymo RNA clean column kit TruSeq RNA sample prep kit Illumina to prepare cDNA libraries post PolyA selection", "Sample 10", "SAMEA113400381", "VANDERBILT UNIVERSITY", "ENA FIRST PUBLIC:2023 07 14T00:23:41Z|ENA LAST UPDATE:2023 07 14T00:23:41Z|External Id:SAMEA113400381|INSDC center alias:VANDERBILT UNIVERSITY|INSDC center name:VANDERBILT UNIVERSITY|INSDC first public:2023 07 14T00:23:41Z|INSDC last update:2023 07 14T00:23:41Z|INSDC status:public|Submitter Id:E MTAB 12901:Sample 10|age:2|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|disease:normal|genotype:abcb4  / |organism part:brain|sample name:E MTAB 12901:Sample 10|scientific name:Danio rerio|sex:female|strain:AB", null, null, null, null, null, null, null, null, "Illumina NovaSeq 6000 paired end sequencing; RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month", "E MTAB 12901:Sample 10 p", "Sample 10 p", "RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month", "brain tissue were dissected from zebrafish and snap frozen until RNA isolation Trizol + zymo RNA clean column kit TruSeq RNA sample prep kit Illumina to prepare cDNA libraries post PolyA selection", "Experimental Factor: genotype:abcb4  / ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP146895", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month", "ENA FIRST PUBLIC:2023 07 14|ENA LAST UPDATE:2023 07 14", "8680-JP-0010_S1_L005_R1_001.fastq.gz 8680-JP-0010_S1_L005_R2_001.fastq.gz", "fastq fastq", 21517298566.0, 71249333.0, "E MTAB 12901:8680 JP 0010 S1 L005 R", "0:151 1:151", "A:5890133399;C:4693794003;G:5193013881;T:5709021540;N:31335743", 151, 151, null, null, 5890133399, 4693794003, 5193013881, 5709021540, 31335743, "ERX10689982", "ERS15395527", "ERA23235100", "VANDERBILT UNIVERSITY|European Nucleotide Archive", "VANDERBILT UNIVERSITY|European Nucleotide Archive", 2, 0.92431, 0.92248, 0.13476, 0.13116, 0.70203, 0.70283, 0.50087, 0.50123, 151, 151, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "United States", "2023-07-14", "Adult", "Adult", "Brain", "Nervous System"], [11720, "ERR11282338", "ERX10689981", "ERS15395526", "ERP146895", "PRJEB61813", "RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month", "E-MTAB-12901", "Other", "The ATP binding cassette subfamily B member 1 ABCB1  encoding a multidrug transporter P glycoprotein  plays a critical role in the efflux of xenobiotics in humans and is implicated in cancer resistance to chemotherapy\u2014however  little information regarding Pgp at the zebrafish. In addition  to study the function of Pgp in the zebrafish brain in the aging process  we performed RNA seq using brain tissue of WT and abcb4 knockout zebrafish at different ages  such as 2 month and 30 month.", "ENA FIRST PUBLIC:2023 07 14|ENA LAST UPDATE:2023 07 14", null, "Protocols: brain tissue were dissected from zebrafish and snap frozen until RNA isolation Trizol + zymo RNA clean column kit TruSeq RNA sample prep kit Illumina to prepare cDNA libraries post PolyA selection", "Sample 5", "SAMEA113400380", "VANDERBILT UNIVERSITY", "ENA FIRST PUBLIC:2023 07 14T00:23:41Z|ENA LAST UPDATE:2023 07 14T00:23:41Z|External Id:SAMEA113400380|INSDC center alias:VANDERBILT UNIVERSITY|INSDC center name:VANDERBILT UNIVERSITY|INSDC first public:2023 07 14T00:23:41Z|INSDC last update:2023 07 14T00:23:41Z|INSDC status:public|Submitter Id:E MTAB 12901:Sample 5|age:30|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|disease:normal|genotype:abcb4  / |organism part:brain|sample name:E MTAB 12901:Sample 5|scientific name:Danio rerio|sex:female|strain:AB", null, null, null, null, null, null, null, null, "Illumina NovaSeq 6000 paired end sequencing; RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month", "E MTAB 12901:Sample 5 p", "Sample 5 p", "RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month", "brain tissue were dissected from zebrafish and snap frozen until RNA isolation Trizol + zymo RNA clean column kit TruSeq RNA sample prep kit Illumina to prepare cDNA libraries post PolyA selection", "Experimental Factor: genotype:abcb4  / ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP146895", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month", "ENA FIRST PUBLIC:2023 07 14|ENA LAST UPDATE:2023 07 14", "8680-JP-0005_S1_L005_R1_001.fastq.gz 8680-JP-0005_S1_L005_R2_001.fastq.gz", "fastq fastq", 25670665306.0, 85002203.0, "E MTAB 12901:8680 JP 0005 S1 L005 R", "0:151 1:151", "A:7004508034;C:5563021332;G:6252648683;T:6813680645;N:36806612", 151, 151, null, null, 7004508034, 5563021332, 6252648683, 6813680645, 36806612, "ERX10689981", "ERS15395526", "ERA23235100", "VANDERBILT UNIVERSITY|European Nucleotide Archive", "VANDERBILT UNIVERSITY|European Nucleotide Archive", 2, 0.92268, 0.92095, 0.13119, 0.12796, 0.70234, 0.70276, 0.51658, 0.51519, 151, 151, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "United States", "2023-07-14", "Adult", "Adult", "Brain", "Nervous System"], [11721, "ERR11282337", "ERX10689980", "ERS15395525", "ERP146895", "PRJEB61813", "RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month", "E-MTAB-12901", "Other", "The ATP binding cassette subfamily B member 1 ABCB1  encoding a multidrug transporter P glycoprotein  plays a critical role in the efflux of xenobiotics in humans and is implicated in cancer resistance to chemotherapy\u2014however  little information regarding Pgp at the zebrafish. In addition  to study the function of Pgp in the zebrafish brain in the aging process  we performed RNA seq using brain tissue of WT and abcb4 knockout zebrafish at different ages  such as 2 month and 30 month.", "ENA FIRST PUBLIC:2023 07 14|ENA LAST UPDATE:2023 07 14", null, "Protocols: brain tissue were dissected from zebrafish and snap frozen until RNA isolation Trizol + zymo RNA clean column kit TruSeq RNA sample prep kit Illumina to prepare cDNA libraries post PolyA selection", "Sample 4", "SAMEA113400379", "VANDERBILT UNIVERSITY", "ENA FIRST PUBLIC:2023 07 14T00:23:41Z|ENA LAST UPDATE:2023 07 14T00:23:41Z|External Id:SAMEA113400379|INSDC center alias:VANDERBILT UNIVERSITY|INSDC center name:VANDERBILT UNIVERSITY|INSDC first public:2023 07 14T00:23:41Z|INSDC last update:2023 07 14T00:23:41Z|INSDC status:public|Submitter Id:E MTAB 12901:Sample 4|age:30|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|disease:normal|genotype:abcb4  / |organism part:brain|sample name:E MTAB 12901:Sample 4|scientific name:Danio rerio|sex:female|strain:AB", null, null, null, null, null, null, null, null, "Illumina NovaSeq 6000 paired end sequencing; RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month", "E MTAB 12901:Sample 4 p", "Sample 4 p", "RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month", "brain tissue were dissected from zebrafish and snap frozen until RNA isolation Trizol + zymo RNA clean column kit TruSeq RNA sample prep kit Illumina to prepare cDNA libraries post PolyA selection", "Experimental Factor: genotype:abcb4  / ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP146895", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month", "ENA FIRST PUBLIC:2023 07 14|ENA LAST UPDATE:2023 07 14", "8680-JP-0004_S1_L005_R1_001.fastq.gz 8680-JP-0004_S1_L005_R2_001.fastq.gz", "fastq fastq", 19678467074.0, 65160487.0, "E MTAB 12901:8680 JP 0004 S1 L005 R", "0:151 1:151", "A:5388172969;C:4290008191;G:4741082570;T:5230699181;N:28504163", 151, 151, null, null, 5388172969, 4290008191, 4741082570, 5230699181, 28504163, "ERX10689980", "ERS15395525", "ERA23235100", "VANDERBILT UNIVERSITY|European Nucleotide Archive", "VANDERBILT UNIVERSITY|European Nucleotide Archive", 2, 0.92019, 0.91807, 0.13779, 0.13325, 0.69739, 0.69682, 0.48956, 0.48353, 151, 151, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "United States", "2023-07-14", "Adult", "Adult", "Brain", "Nervous System"], [11722, "ERR11282336", "ERX10689979", "ERS15395524", "ERP146895", "PRJEB61813", "RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month", "E-MTAB-12901", "Other", "The ATP binding cassette subfamily B member 1 ABCB1  encoding a multidrug transporter P glycoprotein  plays a critical role in the efflux of xenobiotics in humans and is implicated in cancer resistance to chemotherapy\u2014however  little information regarding Pgp at the zebrafish. In addition  to study the function of Pgp in the zebrafish brain in the aging process  we performed RNA seq using brain tissue of WT and abcb4 knockout zebrafish at different ages  such as 2 month and 30 month.", "ENA FIRST PUBLIC:2023 07 14|ENA LAST UPDATE:2023 07 14", null, "Protocols: brain tissue were dissected from zebrafish and snap frozen until RNA isolation Trizol + zymo RNA clean column kit TruSeq RNA sample prep kit Illumina to prepare cDNA libraries post PolyA selection", "Sample 12", "SAMEA113400378", "VANDERBILT UNIVERSITY", "ENA FIRST PUBLIC:2023 07 14T00:23:41Z|ENA LAST UPDATE:2023 07 14T00:23:41Z|External Id:SAMEA113400378|INSDC center alias:VANDERBILT UNIVERSITY|INSDC center name:VANDERBILT UNIVERSITY|INSDC first public:2023 07 14T00:23:41Z|INSDC last update:2023 07 14T00:23:41Z|INSDC status:public|Submitter Id:E MTAB 12901:Sample 12|age:30|broker name:ArrayExpress|common name:zebrafish|developmental stage:adult|disease:normal|genotype:abcb4  / |organism part:brain|sample name:E MTAB 12901:Sample 12|scientific name:Danio rerio|sex:female|strain:AB", null, null, null, null, null, null, null, null, "Illumina NovaSeq 6000 paired end sequencing; RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month", "E MTAB 12901:Sample 12 p", "Sample 12 p", "RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month", "brain tissue were dissected from zebrafish and snap frozen until RNA isolation Trizol + zymo RNA clean column kit TruSeq RNA sample prep kit Illumina to prepare cDNA libraries post PolyA selection", "Experimental Factor: genotype:abcb4  / ", "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "ERP146895", "Illumina NovaSeq 6000 paired end sequencing; RNA seq of brain tissues of WT and abcb4 knockout zebrafish at 2 month and 30 month", "ENA FIRST PUBLIC:2023 07 14|ENA LAST UPDATE:2023 07 14", "8680-JP-0012_S1_L005_R1_001.fastq.gz 8680-JP-0012_S1_L005_R2_001.fastq.gz", "fastq fastq", 19025354324.0, 62997862.0, "E MTAB 12901:8680 JP 0012 S1 L005 R", "0:151 1:151", "A:5203524540;C:4195830684;G:4551047863;T:5047420365;N:27530872", 151, 151, null, null, 5203524540, 4195830684, 4551047863, 5047420365, 27530872, "ERX10689979", "ERS15395524", "ERA23235100", "VANDERBILT UNIVERSITY|European Nucleotide Archive", "VANDERBILT UNIVERSITY|European Nucleotide Archive", 2, 0.92091, 0.92095, 0.13145, 0.12737, 0.69238, 0.69258, 0.50372, 0.48431, 151, 151, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "United States", "2023-07-14", "Adult", "Adult", "Brain", "Nervous System"], [11813, "ERR11788863", "ERX11187331", "ERS16221143", "ERP149944", "PRJEB64780", "A single cell transcriptomic atlas reveals a new myeloid parenchymal population in the zebrafish brain", "E-MTAB-13223", "Transcriptome Analysis", "To understand the cellular basis of the immune compartment of the zebrafish brain we have established reliable protocols for dissociation and prospective isolation of brain leukocytes  using fluorescent transgenic lines. By combining this approach with single cell RNA sequencing  we have generated a gene expression atlas composed of the distinct immune cells present in the homeostatic brain. These analyses revealed the presence of subpopulations of mononuclear phagocytes and other leukocytes  including cell types that have not been  or have been poorly  characterized so far. Here  we present the characterization of a new mononuclear phagocyte population that represents an important fraction among all brain leukocytes.  Adult brain single cell suspensions were prepared from adult Tgp2ry12:GFP; cd45:DsRed fish n=3 and a total of 14 000 cd45:DsRed+ cells were processed for single cell profiling using the 10x Genomics platform.", "ENA FIRST PUBLIC:2023 12 25|ENA LAST UPDATE:2023 12 25", null, "Protocols: FACS sorting of cd45:Dsred cells. Brains from adult Tgp2ry12:GFP; cd45:DsRed fish n=3  were dissected in 0.9X Dulbecco\u2032s Phosphate Buffered Saline DPBS were triturated and treated with Liberase TM at 33\u00b0C for 30 45 minutes  fully dissociated using a syringe with a 26G needle and washed in 2% fetal bovine serum diluted in 0.9X DPBS. Cell suspensions were centrifuged at 290g 4\u00baC 10 min and filtered through a 40\u00b5m nylon mesh. Just before flow cytometry analysis  using calcein violet to exclude dead cells 1\u00b5M  Thermo Fisher. Flow cytometry acquisition and cell sorting was performed on a FACS ARIA II Becton Dickinson. RNA Illumina", "Sample 1", "SAMEA114235870", "Universite Libre de Bruxelles", "ENA FIRST PUBLIC:2023 12 25T01:15:29Z|ENA LAST UPDATE:2023 12 25T01:15:29Z|External Id:SAMEA114235870|INSDC center name:Universite Libre de Bruxelles|INSDC first public:2023 12 25T01:15:29Z|INSDC last update:2023 12 25T01:15:29Z|INSDC status:public|Submitter Id:E MTAB 13223:Sample 1|age:5|broker name:ArrayExpress|cell type:leukocyte|collection date:not collected|common name:zebrafish|developmental stage:adult|genotype:wild type genotype|geographic location country and/or sea:not collected|individual:pool of 3 fish|isolate:not applicable|organism part:brain|sample name:E MTAB 13223:Sample 1|scientific name:Danio rerio|sex:male and female|strain:AB", null, null, null, null, null, null, null, null, "A single cell transcriptomic atlas reveals a new myeloid parenchymal population in the zebrafish brain", "E MTAB 13223:Sample 1 p", "Sample 1 p", "A single cell transcriptomic atlas reveals a new myeloid parenchymal population in the zebrafish brain", "FACS sorting of cd45:Dsred cells. Brains from adult Tgp2ry12:GFP; cd45:DsRed fish n=3  were dissected in 0.9X Dulbecco\u2032s Phosphate Buffered Saline DPBS were triturated and treated with Liberase TM at 33\u00b0C for 30 45 minutes  fully dissociated using a syringe with a 26G needle and washed in 2% fetal bovine serum diluted in 0.9X DPBS. Cell suspensions were centrifuged at 290g 4\u00baC 10 min and filtered through a 40\u00b5m nylon mesh. Just before flow cytometry analysis  using calcein violet to exclude dead cells 1\u00b5M  Thermo Fisher. Flow cytometry acquisition and cell sorting was performed on a FACS ARIA II Becton Dickinson.  RNA Illumina", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "PolyA", "PAIRED", "ILLUMINA", "NextSeq 550", null, "ERP149944", "NextSeq 550 paired end sequencing; A single cell transcriptomic atlas reveals a new myeloid parenchymal population in the zebrafish brain", "ENA FIRST PUBLIC:2023 12 25|ENA LAST UPDATE:2023 12 25", "L41805_Track-81843_R1.fastq.gz L41805_Track-81843_R2.fastq.gz", "fastq fastq", 5545444464.0, 66017196.0, "E MTAB 13223:L41805 Track 81843", "0:28 1:56", "A:1576174024;C:1193175858;G:1221776615;T:1551009004;N:3308963", 28, 56, null, null, 1576174024, 1193175858, 1221776615, 1551009004, 3308963, "ERX11187331", "ERS16221143", "ERA26318932", "European Bioinformatics Institute|European Nucleotide Archive", "European Bioinformatics Institute|European Nucleotide Archive", 2, 0.00738, 0.90151, 0.00285, 0.20553, 0.99072, 0.82402, 0.4645, 0.53817, 28, 56, "T", "B", "sc-like readlen", "illumina", "nextseq", "unknown", "poly_a", "unknown", "sc", "single_cell_droplet", "10x", null, "United Kingdom", "2023-12-25", "Adult", "Adult", "Brain", "Nervous System"], [11814, "ERR11788864", "ERX11187331", "ERS16221143", "ERP149944", "PRJEB64780", "A single cell transcriptomic atlas reveals a new myeloid parenchymal population in the zebrafish brain", "E-MTAB-13223", "Transcriptome Analysis", "To understand the cellular basis of the immune compartment of the zebrafish brain we have established reliable protocols for dissociation and prospective isolation of brain leukocytes  using fluorescent transgenic lines. By combining this approach with single cell RNA sequencing  we have generated a gene expression atlas composed of the distinct immune cells present in the homeostatic brain. These analyses revealed the presence of subpopulations of mononuclear phagocytes and other leukocytes  including cell types that have not been  or have been poorly  characterized so far. Here  we present the characterization of a new mononuclear phagocyte population that represents an important fraction among all brain leukocytes.  Adult brain single cell suspensions were prepared from adult Tgp2ry12:GFP; cd45:DsRed fish n=3 and a total of 14 000 cd45:DsRed+ cells were processed for single cell profiling using the 10x Genomics platform.", "ENA FIRST PUBLIC:2023 12 25|ENA LAST UPDATE:2023 12 25", null, "Protocols: FACS sorting of cd45:Dsred cells. Brains from adult Tgp2ry12:GFP; cd45:DsRed fish n=3  were dissected in 0.9X Dulbecco\u2032s Phosphate Buffered Saline DPBS were triturated and treated with Liberase TM at 33\u00b0C for 30 45 minutes  fully dissociated using a syringe with a 26G needle and washed in 2% fetal bovine serum diluted in 0.9X DPBS. Cell suspensions were centrifuged at 290g 4\u00baC 10 min and filtered through a 40\u00b5m nylon mesh. Just before flow cytometry analysis  using calcein violet to exclude dead cells 1\u00b5M  Thermo Fisher. Flow cytometry acquisition and cell sorting was performed on a FACS ARIA II Becton Dickinson. RNA Illumina", "Sample 1", "SAMEA114235870", "Universite Libre de Bruxelles", "ENA FIRST PUBLIC:2023 12 25T01:15:29Z|ENA LAST UPDATE:2023 12 25T01:15:29Z|External Id:SAMEA114235870|INSDC center name:Universite Libre de Bruxelles|INSDC first public:2023 12 25T01:15:29Z|INSDC last update:2023 12 25T01:15:29Z|INSDC status:public|Submitter Id:E MTAB 13223:Sample 1|age:5|broker name:ArrayExpress|cell type:leukocyte|collection date:not collected|common name:zebrafish|developmental stage:adult|genotype:wild type genotype|geographic location country and/or sea:not collected|individual:pool of 3 fish|isolate:not applicable|organism part:brain|sample name:E MTAB 13223:Sample 1|scientific name:Danio rerio|sex:male and female|strain:AB", null, null, null, null, null, null, null, null, "A single cell transcriptomic atlas reveals a new myeloid parenchymal population in the zebrafish brain", "E MTAB 13223:Sample 1 p", "Sample 1 p", "A single cell transcriptomic atlas reveals a new myeloid parenchymal population in the zebrafish brain", "FACS sorting of cd45:Dsred cells. Brains from adult Tgp2ry12:GFP; cd45:DsRed fish n=3  were dissected in 0.9X Dulbecco\u2032s Phosphate Buffered Saline DPBS were triturated and treated with Liberase TM at 33\u00b0C for 30 45 minutes  fully dissociated using a syringe with a 26G needle and washed in 2% fetal bovine serum diluted in 0.9X DPBS. Cell suspensions were centrifuged at 290g 4\u00baC 10 min and filtered through a 40\u00b5m nylon mesh. Just before flow cytometry analysis  using calcein violet to exclude dead cells 1\u00b5M  Thermo Fisher. Flow cytometry acquisition and cell sorting was performed on a FACS ARIA II Becton Dickinson.  RNA Illumina", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "PolyA", "PAIRED", "ILLUMINA", "NextSeq 550", null, "ERP149944", "NextSeq 550 paired end sequencing; A single cell transcriptomic atlas reveals a new myeloid parenchymal population in the zebrafish brain", "ENA FIRST PUBLIC:2023 12 25|ENA LAST UPDATE:2023 12 25", "L41805_Track-81377_R1.fastq.gz L41805_Track-81377_R2.fastq.gz", "fastq fastq", 3184137096.0, 37906394.0, "E MTAB 13223:L41805 Track 81377", "0:28 1:56", "A:899943184;C:685571078;G:703849582;T:889228559;N:5544693", 28, 56, null, null, 899943184, 685571078, 703849582, 889228559, 5544693, "ERX11187331", "ERS16221143", "ERA26318932", "European Bioinformatics Institute|European Nucleotide Archive", "European Bioinformatics Institute|European Nucleotide Archive", 2, 0.00734, 0.90077, 0.00293, 0.20318, 0.99064, 0.82765, 0.47994, 0.53473, 28, 56, "T", "B", "sc-like readlen", "illumina", "nextseq", "unknown", "poly_a", "unknown", "sc", "single_cell_droplet", "10x", null, "United Kingdom", "2023-12-25", "Adult", "Adult", "Brain", "Nervous System"], [31902, "SRR28763293", "SRX24328715", "SRS21090019", "SRP503258", "PRJNA1102381", "Acidification alters anxiety like behaviour and brain gene expression in zebrafish", "PRJNA1102381", "Other", "CO2 driven acidification of freshwater ecosystems is an accelerating process that can impact aquatic life in the future. Despite their importance in accurately predicting how future freshwater teleost fish will respond to changing environments  molecular mechanisms coordinating the response to elevated CO2 have not been extensively studied. Making use of the zebrafish Danio rerio considered 'tolerant' to acidified waters  we analyzed the behavioural and transcriptomic response to acidification to highlight potential response mechanisms in the brain and the gills which mediate behaviour and perform acid base regulation. Adult zebrafish were exposed to control 500 uatm and elevated CO2 1000 uatm for five days and submitted to Open Field and Novel Object Approach tests  revealing a decrease in anxiety like behaviour in response to elevated CO2. In the brain  acidification caused differential expression of genes involved in cytoskeletal organization  cellular transport  immunity and the visual neural system. In the gills  an elevated expression of genes involved in immune response and oxidoreduction with increasing pCO2 in combination with decreased anxiety like behaviour point to an increase of oxidative stress. However  the absence of differentially expressed genes in gills indicates that this species has adapted to regulating its acid base balance in acidified waters  common in its natural habitat. Our study pinpoints the molecular response to elevated CO2 possibly triggering behavioural changes in zebrafish and suggest that not only marine  but also freshwater fishes will be behaviourally affected by acidification due to rapid climate change.", null, null, null, null, "EB19", null, "replicate name:10|breed:AB|age:4 months|dev stage:adult|collection date:2021 11 14|geo loc name:Hong Kong|sex:not determined|tissue:brain|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of 4mpf Danio rerio brain", "EB19", "EB19", "Total RNA was extracted using the RNeasy Micro Kit Qiagen.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP503258", null, null, "EB19_1.fastq.gz EB19_2.fastq.gz", "fastq fastq", 9436386526.0, 31246313.0, "EB19 1.fastq.gz", "0:151 1:151", "A:2716315062;C:1976294752;G:2147671436;T:2595980360;N:124916", 151, 151, null, null, 2716315062, 1976294752, 2147671436, 2595980360, 124916, "SRX24328715", "SRS21090019", "SRA1849282", "The University of Hong Kong|School of Biological Sciences", "The University of Hong Kong", 2, 0.91409, 0.91345, 0.24896, 0.24788, 0.78056, 0.78279, 0.72332, 0.73168, 151, 151, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2024-04-22", "Adult", "Adult", "Brain", "Nervous System"], [31903, "SRR28763294", "SRX24328714", "SRS21090018", "SRP503258", "PRJNA1102381", "Acidification alters anxiety like behaviour and brain gene expression in zebrafish", "PRJNA1102381", "Other", "CO2 driven acidification of freshwater ecosystems is an accelerating process that can impact aquatic life in the future. Despite their importance in accurately predicting how future freshwater teleost fish will respond to changing environments  molecular mechanisms coordinating the response to elevated CO2 have not been extensively studied. Making use of the zebrafish Danio rerio considered 'tolerant' to acidified waters  we analyzed the behavioural and transcriptomic response to acidification to highlight potential response mechanisms in the brain and the gills which mediate behaviour and perform acid base regulation. Adult zebrafish were exposed to control 500 uatm and elevated CO2 1000 uatm for five days and submitted to Open Field and Novel Object Approach tests  revealing a decrease in anxiety like behaviour in response to elevated CO2. In the brain  acidification caused differential expression of genes involved in cytoskeletal organization  cellular transport  immunity and the visual neural system. In the gills  an elevated expression of genes involved in immune response and oxidoreduction with increasing pCO2 in combination with decreased anxiety like behaviour point to an increase of oxidative stress. However  the absence of differentially expressed genes in gills indicates that this species has adapted to regulating its acid base balance in acidified waters  common in its natural habitat. Our study pinpoints the molecular response to elevated CO2 possibly triggering behavioural changes in zebrafish and suggest that not only marine  but also freshwater fishes will be behaviourally affected by acidification due to rapid climate change.", null, null, null, null, "EB18", null, "replicate name:9|breed:AB|age:4 months|dev stage:adult|collection date:2021 11 08|geo loc name:Hong Kong|sex:not determined|tissue:brain|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of 4mpf Danio rerio brain", "EB18", "EB18", "Total RNA was extracted using the RNeasy Micro Kit Qiagen.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP503258", null, null, "EB18_1.fastq.gz EB18_2.fastq.gz", "fastq fastq", 9118420390.0, 30193445.0, "EB18 1.fastq.gz", "0:151 1:151", "A:2629035452;C:1916588061;G:2003273132;T:2569401842;N:121903", 151, 151, null, null, 2629035452, 1916588061, 2003273132, 2569401842, 121903, "SRX24328714", "SRS21090018", "SRA1849282", "The University of Hong Kong|School of Biological Sciences", "The University of Hong Kong", 2, 0.92136, 0.91989, 0.22403, 0.22513, 0.72318, 0.72344, 0.58479, 0.59137, 151, 151, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2024-04-22", "Adult", "Adult", "Brain", "Nervous System"], [31904, "SRR28763295", "SRX24328713", "SRS21090017", "SRP503258", "PRJNA1102381", "Acidification alters anxiety like behaviour and brain gene expression in zebrafish", "PRJNA1102381", "Other", "CO2 driven acidification of freshwater ecosystems is an accelerating process that can impact aquatic life in the future. Despite their importance in accurately predicting how future freshwater teleost fish will respond to changing environments  molecular mechanisms coordinating the response to elevated CO2 have not been extensively studied. Making use of the zebrafish Danio rerio considered 'tolerant' to acidified waters  we analyzed the behavioural and transcriptomic response to acidification to highlight potential response mechanisms in the brain and the gills which mediate behaviour and perform acid base regulation. Adult zebrafish were exposed to control 500 uatm and elevated CO2 1000 uatm for five days and submitted to Open Field and Novel Object Approach tests  revealing a decrease in anxiety like behaviour in response to elevated CO2. In the brain  acidification caused differential expression of genes involved in cytoskeletal organization  cellular transport  immunity and the visual neural system. In the gills  an elevated expression of genes involved in immune response and oxidoreduction with increasing pCO2 in combination with decreased anxiety like behaviour point to an increase of oxidative stress. However  the absence of differentially expressed genes in gills indicates that this species has adapted to regulating its acid base balance in acidified waters  common in its natural habitat. Our study pinpoints the molecular response to elevated CO2 possibly triggering behavioural changes in zebrafish and suggest that not only marine  but also freshwater fishes will be behaviourally affected by acidification due to rapid climate change.", null, null, null, null, "EB13", null, "replicate name:8|breed:AB|age:4 months|dev stage:adult|collection date:2021 11 08|geo loc name:Hong Kong|sex:not determined|tissue:brain|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of 4mpf Danio rerio brain", "EB13", "EB13", "Total RNA was extracted using the RNeasy Micro Kit Qiagen.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP503258", null, null, "EB13_1.fastq.gz EB13_2.fastq.gz", "fastq fastq", 9079809388.0, 30065594.0, "EB13 1.fastq.gz", "0:151 1:151", "A:2664300477;C:1849456779;G:1954969464;T:2610962453;N:120215", 151, 151, null, null, 2664300477, 1849456779, 1954969464, 2610962453, 120215, "SRX24328713", "SRS21090017", "SRA1849282", "The University of Hong Kong|School of Biological Sciences", "The University of Hong Kong", 2, 0.92157, 0.92274, 0.20709, 0.20463, 0.77609, 0.77962, 0.77474, 0.76073, 151, 151, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2024-04-22", "Adult", "Adult", "Brain", "Nervous System"], [31905, "SRR28763296", "SRX24328712", "SRS21090016", "SRP503258", "PRJNA1102381", "Acidification alters anxiety like behaviour and brain gene expression in zebrafish", "PRJNA1102381", "Other", "CO2 driven acidification of freshwater ecosystems is an accelerating process that can impact aquatic life in the future. Despite their importance in accurately predicting how future freshwater teleost fish will respond to changing environments  molecular mechanisms coordinating the response to elevated CO2 have not been extensively studied. Making use of the zebrafish Danio rerio considered 'tolerant' to acidified waters  we analyzed the behavioural and transcriptomic response to acidification to highlight potential response mechanisms in the brain and the gills which mediate behaviour and perform acid base regulation. Adult zebrafish were exposed to control 500 uatm and elevated CO2 1000 uatm for five days and submitted to Open Field and Novel Object Approach tests  revealing a decrease in anxiety like behaviour in response to elevated CO2. In the brain  acidification caused differential expression of genes involved in cytoskeletal organization  cellular transport  immunity and the visual neural system. In the gills  an elevated expression of genes involved in immune response and oxidoreduction with increasing pCO2 in combination with decreased anxiety like behaviour point to an increase of oxidative stress. However  the absence of differentially expressed genes in gills indicates that this species has adapted to regulating its acid base balance in acidified waters  common in its natural habitat. Our study pinpoints the molecular response to elevated CO2 possibly triggering behavioural changes in zebrafish and suggest that not only marine  but also freshwater fishes will be behaviourally affected by acidification due to rapid climate change.", null, null, null, null, "EB11", null, "replicate name:7|breed:AB|age:4 months|dev stage:adult|collection date:2021 11 08|geo loc name:Hong Kong|sex:not determined|tissue:brain|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of 4mpf Danio rerio brain", "EB11", "EB11", "Total RNA was extracted using the RNeasy Micro Kit Qiagen.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP503258", null, null, "EB11_1.fastq.gz EB11_2.fastq.gz", "fastq fastq", 9504832712.0, 31472956.0, "EB11 1.fastq.gz", "0:151 1:151", "A:2795532595;C:1925063954;G:2051665162;T:2732443741;N:127260", 151, 151, null, null, 2795532595, 1925063954, 2051665162, 2732443741, 127260, "SRX24328712", "SRS21090016", "SRA1849282", "The University of Hong Kong|School of Biological Sciences", "The University of Hong Kong", 2, 0.93395, 0.93446, 0.15772, 0.15556, 0.87117, 0.87462, 0.89532, 0.89681, 151, 151, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2024-04-22", "Adult", "Adult", "Brain", "Nervous System"], [31906, "SRR28763297", "SRX24328711", "SRS21090015", "SRP503258", "PRJNA1102381", "Acidification alters anxiety like behaviour and brain gene expression in zebrafish", "PRJNA1102381", "Other", "CO2 driven acidification of freshwater ecosystems is an accelerating process that can impact aquatic life in the future. Despite their importance in accurately predicting how future freshwater teleost fish will respond to changing environments  molecular mechanisms coordinating the response to elevated CO2 have not been extensively studied. Making use of the zebrafish Danio rerio considered 'tolerant' to acidified waters  we analyzed the behavioural and transcriptomic response to acidification to highlight potential response mechanisms in the brain and the gills which mediate behaviour and perform acid base regulation. Adult zebrafish were exposed to control 500 uatm and elevated CO2 1000 uatm for five days and submitted to Open Field and Novel Object Approach tests  revealing a decrease in anxiety like behaviour in response to elevated CO2. In the brain  acidification caused differential expression of genes involved in cytoskeletal organization  cellular transport  immunity and the visual neural system. In the gills  an elevated expression of genes involved in immune response and oxidoreduction with increasing pCO2 in combination with decreased anxiety like behaviour point to an increase of oxidative stress. However  the absence of differentially expressed genes in gills indicates that this species has adapted to regulating its acid base balance in acidified waters  common in its natural habitat. Our study pinpoints the molecular response to elevated CO2 possibly triggering behavioural changes in zebrafish and suggest that not only marine  but also freshwater fishes will be behaviourally affected by acidification due to rapid climate change.", null, null, null, null, "EB10", null, "replicate name:6|breed:AB|age:4 months|dev stage:adult|collection date:2021 11 08|geo loc name:Hong Kong|sex:not determined|tissue:brain|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of 4mpf Danio rerio brain", "EB10", "EB10", "Total RNA was extracted using the RNeasy Micro Kit Qiagen.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP503258", null, null, "EB10_1.fastq.gz EB10_2.fastq.gz", "fastq fastq", 9789274734.0, 32414817.0, "EB10 1.fastq.gz", "0:151 1:151", "A:2824956587;C:2042095073;G:2194234020;T:2727859573;N:129481", 151, 151, null, null, 2824956587, 2042095073, 2194234020, 2727859573, 129481, "SRX24328711", "SRS21090015", "SRA1849282", "The University of Hong Kong|School of Biological Sciences", "The University of Hong Kong", 2, 0.92285, 0.9232, 0.22353, 0.22316, 0.76658, 0.76723, 0.70407, 0.36056, 151, 151, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2024-04-22", "Adult", "Adult", "Brain", "Nervous System"], [31907, "SRR28763298", "SRX24328710", "SRS21090014", "SRP503258", "PRJNA1102381", "Acidification alters anxiety like behaviour and brain gene expression in zebrafish", "PRJNA1102381", "Other", "CO2 driven acidification of freshwater ecosystems is an accelerating process that can impact aquatic life in the future. Despite their importance in accurately predicting how future freshwater teleost fish will respond to changing environments  molecular mechanisms coordinating the response to elevated CO2 have not been extensively studied. Making use of the zebrafish Danio rerio considered 'tolerant' to acidified waters  we analyzed the behavioural and transcriptomic response to acidification to highlight potential response mechanisms in the brain and the gills which mediate behaviour and perform acid base regulation. Adult zebrafish were exposed to control 500 uatm and elevated CO2 1000 uatm for five days and submitted to Open Field and Novel Object Approach tests  revealing a decrease in anxiety like behaviour in response to elevated CO2. In the brain  acidification caused differential expression of genes involved in cytoskeletal organization  cellular transport  immunity and the visual neural system. In the gills  an elevated expression of genes involved in immune response and oxidoreduction with increasing pCO2 in combination with decreased anxiety like behaviour point to an increase of oxidative stress. However  the absence of differentially expressed genes in gills indicates that this species has adapted to regulating its acid base balance in acidified waters  common in its natural habitat. Our study pinpoints the molecular response to elevated CO2 possibly triggering behavioural changes in zebrafish and suggest that not only marine  but also freshwater fishes will be behaviourally affected by acidification due to rapid climate change.", null, null, null, null, "EB7", null, "replicate name:5|breed:AB|age:4 months|dev stage:adult|collection date:2021 11 02|geo loc name:Hong Kong|sex:not determined|tissue:brain|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of 4mpf Danio rerio brain", "EB7", "EB7", "Total RNA was extracted using the RNeasy Micro Kit Qiagen.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP503258", null, null, "EB7_1.fastq.gz EB7_2.fastq.gz", "fastq fastq", 10834721120.0, 35876560.0, "EB7 1.fastq.gz", "0:151 1:151", "A:3080234341;C:2317625501;G:2459979119;T:2976734982;N:147177", 151, 151, null, null, 3080234341, 2317625501, 2459979119, 2976734982, 147177, "SRX24328710", "SRS21090014", "SRA1849282", "The University of Hong Kong|School of Biological Sciences", "The University of Hong Kong", 2, 0.91597, 0.91208, 0.20246, 0.20371, 0.70711, 0.70737, 0.46676, 0.54014, 151, 151, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2024-04-22", "Adult", "Adult", "Brain", "Nervous System"], [31908, "SRR28763299", "SRX24328709", "SRS21090013", "SRP503258", "PRJNA1102381", "Acidification alters anxiety like behaviour and brain gene expression in zebrafish", "PRJNA1102381", "Other", "CO2 driven acidification of freshwater ecosystems is an accelerating process that can impact aquatic life in the future. Despite their importance in accurately predicting how future freshwater teleost fish will respond to changing environments  molecular mechanisms coordinating the response to elevated CO2 have not been extensively studied. Making use of the zebrafish Danio rerio considered 'tolerant' to acidified waters  we analyzed the behavioural and transcriptomic response to acidification to highlight potential response mechanisms in the brain and the gills which mediate behaviour and perform acid base regulation. Adult zebrafish were exposed to control 500 uatm and elevated CO2 1000 uatm for five days and submitted to Open Field and Novel Object Approach tests  revealing a decrease in anxiety like behaviour in response to elevated CO2. In the brain  acidification caused differential expression of genes involved in cytoskeletal organization  cellular transport  immunity and the visual neural system. In the gills  an elevated expression of genes involved in immune response and oxidoreduction with increasing pCO2 in combination with decreased anxiety like behaviour point to an increase of oxidative stress. However  the absence of differentially expressed genes in gills indicates that this species has adapted to regulating its acid base balance in acidified waters  common in its natural habitat. Our study pinpoints the molecular response to elevated CO2 possibly triggering behavioural changes in zebrafish and suggest that not only marine  but also freshwater fishes will be behaviourally affected by acidification due to rapid climate change.", null, null, null, null, "EB6", null, "replicate name:4|breed:AB|age:4 months|dev stage:adult|collection date:2021 11 02|geo loc name:Hong Kong|sex:not determined|tissue:brain|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of 4mpf Danio rerio brain", "EB6", "EB6", "Total RNA was extracted using the RNeasy Micro Kit Qiagen.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP503258", null, null, "EB6_1.fastq.gz EB6_2.fastq.gz", "fastq fastq", 9741721512.0, 32257356.0, "EB6 1.fastq.gz", "0:151 1:151", "A:2786663368;C:2058810098;G:2182236675;T:2713885545;N:125826", 151, 151, null, null, 2786663368, 2058810098, 2182236675, 2713885545, 125826, "SRX24328709", "SRS21090013", "SRA1849282", "The University of Hong Kong|School of Biological Sciences", "The University of Hong Kong", 2, 0.9221, 0.91676, 0.20096, 0.20109, 0.71885, 0.72109, 0.5729, 0.56637, 151, 151, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2024-04-22", "Adult", "Adult", "Brain", "Nervous System"], [31915, "SRR28763306", "SRX24328702", "SRS21090006", "SRP503258", "PRJNA1102381", "Acidification alters anxiety like behaviour and brain gene expression in zebrafish", "PRJNA1102381", "Other", "CO2 driven acidification of freshwater ecosystems is an accelerating process that can impact aquatic life in the future. Despite their importance in accurately predicting how future freshwater teleost fish will respond to changing environments  molecular mechanisms coordinating the response to elevated CO2 have not been extensively studied. Making use of the zebrafish Danio rerio considered 'tolerant' to acidified waters  we analyzed the behavioural and transcriptomic response to acidification to highlight potential response mechanisms in the brain and the gills which mediate behaviour and perform acid base regulation. Adult zebrafish were exposed to control 500 uatm and elevated CO2 1000 uatm for five days and submitted to Open Field and Novel Object Approach tests  revealing a decrease in anxiety like behaviour in response to elevated CO2. In the brain  acidification caused differential expression of genes involved in cytoskeletal organization  cellular transport  immunity and the visual neural system. In the gills  an elevated expression of genes involved in immune response and oxidoreduction with increasing pCO2 in combination with decreased anxiety like behaviour point to an increase of oxidative stress. However  the absence of differentially expressed genes in gills indicates that this species has adapted to regulating its acid base balance in acidified waters  common in its natural habitat. Our study pinpoints the molecular response to elevated CO2 possibly triggering behavioural changes in zebrafish and suggest that not only marine  but also freshwater fishes will be behaviourally affected by acidification due to rapid climate change.", null, null, null, null, "EB5", null, "replicate name:3|breed:AB|age:4 months|dev stage:adult|collection date:2021 11 02|geo loc name:Hong Kong|sex:not determined|tissue:brain|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of 4mpf Danio rerio brain", "EB5", "EB5", "Total RNA was extracted using the RNeasy Micro Kit Qiagen.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP503258", null, null, "EB5_1.fastq.gz EB5_2.fastq.gz", "fastq fastq", 10009088756.0, 33142678.0, "EB5 1.fastq.gz", "0:151 1:151", "A:2928623095;C:2061836360;G:2174039537;T:2844456450;N:133314", 151, 151, null, null, 2928623095, 2061836360, 2174039537, 2844456450, 133314, "SRX24328702", "SRS21090006", "SRA1849282", "The University of Hong Kong|School of Biological Sciences", "The University of Hong Kong", 2, 0.9185, 0.91663, 0.21613, 0.21748, 0.71829, 0.71967, 0.56772, 0.56625, 151, 151, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2024-04-22", "Adult", "Adult", "Brain", "Nervous System"], [31922, "SRR28763313", "SRX24328695", "SRS21089999", "SRP503258", "PRJNA1102381", "Acidification alters anxiety like behaviour and brain gene expression in zebrafish", "PRJNA1102381", "Other", "CO2 driven acidification of freshwater ecosystems is an accelerating process that can impact aquatic life in the future. Despite their importance in accurately predicting how future freshwater teleost fish will respond to changing environments  molecular mechanisms coordinating the response to elevated CO2 have not been extensively studied. Making use of the zebrafish Danio rerio considered 'tolerant' to acidified waters  we analyzed the behavioural and transcriptomic response to acidification to highlight potential response mechanisms in the brain and the gills which mediate behaviour and perform acid base regulation. Adult zebrafish were exposed to control 500 uatm and elevated CO2 1000 uatm for five days and submitted to Open Field and Novel Object Approach tests  revealing a decrease in anxiety like behaviour in response to elevated CO2. In the brain  acidification caused differential expression of genes involved in cytoskeletal organization  cellular transport  immunity and the visual neural system. In the gills  an elevated expression of genes involved in immune response and oxidoreduction with increasing pCO2 in combination with decreased anxiety like behaviour point to an increase of oxidative stress. However  the absence of differentially expressed genes in gills indicates that this species has adapted to regulating its acid base balance in acidified waters  common in its natural habitat. Our study pinpoints the molecular response to elevated CO2 possibly triggering behavioural changes in zebrafish and suggest that not only marine  but also freshwater fishes will be behaviourally affected by acidification due to rapid climate change.", null, null, null, null, "EB27", null, "replicate name:14|breed:AB|age:4 months|dev stage:adult|collection date:2021 11 14|geo loc name:Hong Kong|sex:not determined|tissue:brain|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of 4mpf Danio rerio brain", "EB27", "EB27", "Total RNA was extracted using the RNeasy Micro Kit Qiagen.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP503258", null, null, "EB27_1.fastq.gz EB27_2.fastq.gz", "fastq fastq", 9161773094.0, 30336997.0, "EB27 1.fastq.gz", "0:151 1:151", "A:2605950329;C:1969811465;G:2071575244;T:2514313551;N:122505", 151, 151, null, null, 2605950329, 1969811465, 2071575244, 2514313551, 122505, "SRX24328695", "SRS21089999", "SRA1849282", "The University of Hong Kong|School of Biological Sciences", "The University of Hong Kong", 2, 0.92523, 0.92363, 0.21064, 0.21169, 0.70964, 0.70991, 0.55438, 0.55426, 151, 151, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2024-04-22", "Adult", "Adult", "Brain", "Nervous System"], [31923, "SRR28763314", "SRX24328694", "SRS21089998", "SRP503258", "PRJNA1102381", "Acidification alters anxiety like behaviour and brain gene expression in zebrafish", "PRJNA1102381", "Other", "CO2 driven acidification of freshwater ecosystems is an accelerating process that can impact aquatic life in the future. Despite their importance in accurately predicting how future freshwater teleost fish will respond to changing environments  molecular mechanisms coordinating the response to elevated CO2 have not been extensively studied. Making use of the zebrafish Danio rerio considered 'tolerant' to acidified waters  we analyzed the behavioural and transcriptomic response to acidification to highlight potential response mechanisms in the brain and the gills which mediate behaviour and perform acid base regulation. Adult zebrafish were exposed to control 500 uatm and elevated CO2 1000 uatm for five days and submitted to Open Field and Novel Object Approach tests  revealing a decrease in anxiety like behaviour in response to elevated CO2. In the brain  acidification caused differential expression of genes involved in cytoskeletal organization  cellular transport  immunity and the visual neural system. In the gills  an elevated expression of genes involved in immune response and oxidoreduction with increasing pCO2 in combination with decreased anxiety like behaviour point to an increase of oxidative stress. However  the absence of differentially expressed genes in gills indicates that this species has adapted to regulating its acid base balance in acidified waters  common in its natural habitat. Our study pinpoints the molecular response to elevated CO2 possibly triggering behavioural changes in zebrafish and suggest that not only marine  but also freshwater fishes will be behaviourally affected by acidification due to rapid climate change.", null, null, null, null, "EB26", null, "replicate name:13|breed:AB|age:4 months|dev stage:adult|collection date:2021 11 14|geo loc name:Hong Kong|sex:not determined|tissue:brain|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of 4mpf Danio rerio brain", "EB26", "EB26", "Total RNA was extracted using the RNeasy Micro Kit Qiagen.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP503258", null, null, "EB26_1.fastq.gz EB26_2.fastq.gz", "fastq fastq", 9240227560.0, 30596780.0, "EB26 1.fastq.gz", "0:151 1:151", "A:2642944689;C:1958167494;G:2078976911;T:2560016979;N:121487", 151, 151, null, null, 2642944689, 1958167494, 2078976911, 2560016979, 121487, "SRX24328694", "SRS21089998", "SRA1849282", "The University of Hong Kong|School of Biological Sciences", "The University of Hong Kong", 2, 0.92264, 0.92192, 0.21746, 0.21734, 0.73292, 0.73383, 0.62606, 0.61636, 151, 151, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2024-04-22", "Adult", "Adult", "Brain", "Nervous System"], [31924, "SRR28763315", "SRX24328693", "SRS21089997", "SRP503258", "PRJNA1102381", "Acidification alters anxiety like behaviour and brain gene expression in zebrafish", "PRJNA1102381", "Other", "CO2 driven acidification of freshwater ecosystems is an accelerating process that can impact aquatic life in the future. Despite their importance in accurately predicting how future freshwater teleost fish will respond to changing environments  molecular mechanisms coordinating the response to elevated CO2 have not been extensively studied. Making use of the zebrafish Danio rerio considered 'tolerant' to acidified waters  we analyzed the behavioural and transcriptomic response to acidification to highlight potential response mechanisms in the brain and the gills which mediate behaviour and perform acid base regulation. Adult zebrafish were exposed to control 500 uatm and elevated CO2 1000 uatm for five days and submitted to Open Field and Novel Object Approach tests  revealing a decrease in anxiety like behaviour in response to elevated CO2. In the brain  acidification caused differential expression of genes involved in cytoskeletal organization  cellular transport  immunity and the visual neural system. In the gills  an elevated expression of genes involved in immune response and oxidoreduction with increasing pCO2 in combination with decreased anxiety like behaviour point to an increase of oxidative stress. However  the absence of differentially expressed genes in gills indicates that this species has adapted to regulating its acid base balance in acidified waters  common in its natural habitat. Our study pinpoints the molecular response to elevated CO2 possibly triggering behavioural changes in zebrafish and suggest that not only marine  but also freshwater fishes will be behaviourally affected by acidification due to rapid climate change.", null, null, null, null, "EB25", null, "replicate name:12|breed:AB|age:4 months|dev stage:adult|collection date:2021 11 14|geo loc name:Hong Kong|sex:not determined|tissue:brain|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of 4mpf Danio rerio brain", "EB25", "EB25", "Total RNA was extracted using the RNeasy Micro Kit Qiagen.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP503258", null, null, "EB25_1.fastq.gz EB25_2.fastq.gz", "fastq fastq", 9625587110.0, 31872805.0, "EB25 1.fastq.gz", "0:151 1:151", "A:2795560694;C:1995787311;G:2099611038;T:2734501492;N:126575", 151, 151, null, null, 2795560694, 1995787311, 2099611038, 2734501492, 126575, "SRX24328693", "SRS21089997", "SRA1849282", "The University of Hong Kong|School of Biological Sciences", "The University of Hong Kong", 2, 0.92658, 0.92669, 0.20737, 0.20889, 0.74637, 0.74852, 0.665, 0.67094, 151, 151, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2024-04-22", "Adult", "Adult", "Brain", "Nervous System"], [31925, "SRR28763316", "SRX24328692", "SRS21089996", "SRP503258", "PRJNA1102381", "Acidification alters anxiety like behaviour and brain gene expression in zebrafish", "PRJNA1102381", "Other", "CO2 driven acidification of freshwater ecosystems is an accelerating process that can impact aquatic life in the future. Despite their importance in accurately predicting how future freshwater teleost fish will respond to changing environments  molecular mechanisms coordinating the response to elevated CO2 have not been extensively studied. Making use of the zebrafish Danio rerio considered 'tolerant' to acidified waters  we analyzed the behavioural and transcriptomic response to acidification to highlight potential response mechanisms in the brain and the gills which mediate behaviour and perform acid base regulation. Adult zebrafish were exposed to control 500 uatm and elevated CO2 1000 uatm for five days and submitted to Open Field and Novel Object Approach tests  revealing a decrease in anxiety like behaviour in response to elevated CO2. In the brain  acidification caused differential expression of genes involved in cytoskeletal organization  cellular transport  immunity and the visual neural system. In the gills  an elevated expression of genes involved in immune response and oxidoreduction with increasing pCO2 in combination with decreased anxiety like behaviour point to an increase of oxidative stress. However  the absence of differentially expressed genes in gills indicates that this species has adapted to regulating its acid base balance in acidified waters  common in its natural habitat. Our study pinpoints the molecular response to elevated CO2 possibly triggering behavioural changes in zebrafish and suggest that not only marine  but also freshwater fishes will be behaviourally affected by acidification due to rapid climate change.", null, null, null, null, "EB21", null, "replicate name:11|breed:AB|age:4 months|dev stage:adult|collection date:2021 11 14|geo loc name:Hong Kong|sex:not determined|tissue:brain|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of 4mpf Danio rerio brain", "EB21", "EB21", "Total RNA was extracted using the RNeasy Micro Kit Qiagen.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP503258", null, null, "EB21_1.fastq.gz EB21_2.fastq.gz", "fastq fastq", 9252105220.0, 30636110.0, "EB21 1.fastq.gz", "0:151 1:151", "A:2713520368;C:1892341723;G:1978899105;T:2667222416;N:121608", 151, 151, null, null, 2713520368, 1892341723, 1978899105, 2667222416, 121608, "SRX24328692", "SRS21089996", "SRA1849282", "The University of Hong Kong|School of Biological Sciences", "The University of Hong Kong", 2, 0.92204, 0.92307, 0.20614, 0.20487, 0.77561, 0.77956, 0.75506, 0.73666, 151, 151, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2024-04-22", "Adult", "Adult", "Brain", "Nervous System"], [31926, "SRR28763317", "SRX24328691", "SRS21089995", "SRP503258", "PRJNA1102381", "Acidification alters anxiety like behaviour and brain gene expression in zebrafish", "PRJNA1102381", "Other", "CO2 driven acidification of freshwater ecosystems is an accelerating process that can impact aquatic life in the future. Despite their importance in accurately predicting how future freshwater teleost fish will respond to changing environments  molecular mechanisms coordinating the response to elevated CO2 have not been extensively studied. Making use of the zebrafish Danio rerio considered 'tolerant' to acidified waters  we analyzed the behavioural and transcriptomic response to acidification to highlight potential response mechanisms in the brain and the gills which mediate behaviour and perform acid base regulation. Adult zebrafish were exposed to control 500 uatm and elevated CO2 1000 uatm for five days and submitted to Open Field and Novel Object Approach tests  revealing a decrease in anxiety like behaviour in response to elevated CO2. In the brain  acidification caused differential expression of genes involved in cytoskeletal organization  cellular transport  immunity and the visual neural system. In the gills  an elevated expression of genes involved in immune response and oxidoreduction with increasing pCO2 in combination with decreased anxiety like behaviour point to an increase of oxidative stress. However  the absence of differentially expressed genes in gills indicates that this species has adapted to regulating its acid base balance in acidified waters  common in its natural habitat. Our study pinpoints the molecular response to elevated CO2 possibly triggering behavioural changes in zebrafish and suggest that not only marine  but also freshwater fishes will be behaviourally affected by acidification due to rapid climate change.", null, null, null, null, "EB4", null, "replicate name:2|breed:AB|age:4 months|dev stage:adult|collection date:2021 11 02|geo loc name:Hong Kong|sex:not determined|tissue:brain|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of 4mpf Danio rerio brain", "EB4", "EB4", "Total RNA was extracted using the RNeasy Micro Kit Qiagen.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP503258", null, null, "EB4_1.fastq.gz EB4_2.fastq.gz", "fastq fastq", 10173670300.0, 33687650.0, "EB4 1.fastq.gz", "0:151 1:151", "A:2946513536;C:2116612054;G:2242572059;T:2867836703;N:135948", 151, 151, null, null, 2946513536, 2116612054, 2242572059, 2867836703, 135948, "SRX24328691", "SRS21089995", "SRA1849282", "The University of Hong Kong|School of Biological Sciences", "The University of Hong Kong", 2, 0.92771, 0.92647, 0.2039, 0.20424, 0.74485, 0.74679, 0.67237, 0.67051, 151, 151, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2024-04-22", "Adult", "Adult", "Brain", "Nervous System"], [31927, "SRR28763318", "SRX24328690", "SRS21089994", "SRP503258", "PRJNA1102381", "Acidification alters anxiety like behaviour and brain gene expression in zebrafish", "PRJNA1102381", "Other", "CO2 driven acidification of freshwater ecosystems is an accelerating process that can impact aquatic life in the future. Despite their importance in accurately predicting how future freshwater teleost fish will respond to changing environments  molecular mechanisms coordinating the response to elevated CO2 have not been extensively studied. Making use of the zebrafish Danio rerio considered 'tolerant' to acidified waters  we analyzed the behavioural and transcriptomic response to acidification to highlight potential response mechanisms in the brain and the gills which mediate behaviour and perform acid base regulation. Adult zebrafish were exposed to control 500 uatm and elevated CO2 1000 uatm for five days and submitted to Open Field and Novel Object Approach tests  revealing a decrease in anxiety like behaviour in response to elevated CO2. In the brain  acidification caused differential expression of genes involved in cytoskeletal organization  cellular transport  immunity and the visual neural system. In the gills  an elevated expression of genes involved in immune response and oxidoreduction with increasing pCO2 in combination with decreased anxiety like behaviour point to an increase of oxidative stress. However  the absence of differentially expressed genes in gills indicates that this species has adapted to regulating its acid base balance in acidified waters  common in its natural habitat. Our study pinpoints the molecular response to elevated CO2 possibly triggering behavioural changes in zebrafish and suggest that not only marine  but also freshwater fishes will be behaviourally affected by acidification due to rapid climate change.", null, null, null, null, "EB3", null, "replicate name:1|breed:AB|age:4 months|dev stage:adult|collection date:2021 11 02|geo loc name:Hong Kong|sex:not determined|tissue:brain|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of 4mpf Danio rerio brain", "EB3", "EB3", "Total RNA was extracted using the RNeasy Micro Kit Qiagen.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP503258", null, null, "EB3_1.fastq.gz EB3_2.fastq.gz", "fastq fastq", 9627538030.0, 31879265.0, "EB3 1.fastq.gz", "0:151 1:151", "A:2735805884;C:2063838700;G:2168523582;T:2659242105;N:127759", 151, 151, null, null, 2735805884, 2063838700, 2168523582, 2659242105, 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pipefish spleen and the small white organ of the lined seahorse.", "PRJNA799842", "Other", "To explore the function of the gene in spleen development  we generated a knockout zebrafish line. To investigate whether the phenotypic consequences in the seahorses were caused by a missense mutation in this gene  we generated a point mutation zebrafish line by CRISPR/Cas9 mediated homologous recombination HR. RNA seq of these samples tissues brain  liver  kidney  and intestine were analyzed. In addition  to clarify the splenic phenotype of the Syngnathidaes  the transcriptomic profiles of the S. biaculeatus spleens and the H. erectus small white organ were also sampled and sequenced.", null, null, null, null, "WT br 4", null, "strain:AB type|isolate:wild type|breed:WT br 4|age:3 month|dev stage:NA|sex:NA|tissue:brain|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA seq of zebrafish", "WT br 4", "WT br 4", "RNA seq", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP356388", null, "loader:fastq load.py", "Daniorerio_WT-br-4_good_1.fq.gz Daniorerio_WT-br-4_good_2.fq.gz", "fastq fastq", 7496868324.0, 25083735.0, "Daniorerio WT br 4 good 1.fq.gz", "0:149.44 1:149.44", "A:2075060953;C:1661290867;G:1697443641;T:2062703710;N:369153", 149, 149, null, null, 2075060953, 1661290867, 1697443641, 2062703710, 369153, "SRX13877717", "SRS11747891", "SRA1360632", "South China Sea Institute of Oceanology|CAS Key Laboratory of Tropical Marine Bio-Resource", "South China Sea Institute of Oceanology", 2, 0.92105, 0.9212, 0.17618, 0.17502, 0.68844, 0.69035, 0.48965, 0.48899, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2022-01-27", "Adult", "Adult", "Brain", "Nervous System"], [68350, "SRR17714693", "SRX13877716", "SRS11747889", "SRP356388", "PRJNA799842", "RNA seq of CRISPR/Cas9 mediated genome editing zebrafishes; the alligator pipefish spleen and the small white organ of the lined seahorse.", "PRJNA799842", "Other", "To explore the function of the gene in spleen development  we generated a knockout zebrafish line. To investigate whether the phenotypic consequences in the seahorses were caused by a missense mutation in this gene  we generated a point mutation zebrafish line by CRISPR/Cas9 mediated homologous recombination HR. RNA seq of these samples tissues brain  liver  kidney  and intestine were analyzed. In addition  to clarify the splenic phenotype of the Syngnathidaes  the transcriptomic profiles of the S. biaculeatus spleens and the H. erectus small white organ were also sampled and sequenced.", null, null, null, null, "WT br 3", null, "strain:AB type|isolate:wild type|breed:WT br 3|age:3 month|dev stage:NA|sex:NA|tissue:brain|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA seq of zebrafish", "WT br 3", "WT br 3", "RNA seq", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP356388", null, "loader:fastq load.py", "Daniorerio_WT-br-3_good_1.fq.gz Daniorerio_WT-br-3_good_2.fq.gz", "fastq fastq", 6073470194.0, 20313710.0, "Daniorerio WT br 3 good 1.fq.gz", "0:149.49 1:149.49", "A:1713896651;C:1313013182;G:1348015489;T:1698243797;N:301075", 149, 149, null, null, 1713896651, 1313013182, 1348015489, 1698243797, 301075, "SRX13877716", "SRS11747889", "SRA1360632", "South China Sea Institute of Oceanology|CAS Key Laboratory of Tropical Marine Bio-Resource", "South China Sea Institute of Oceanology", 2, 0.91892, 0.91619, 0.18079, 0.17925, 0.68864, 0.69039, 0.49157, 0.48629, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2022-01-27", "Adult", "Adult", "Brain", "Nervous System"], [68351, "SRR17714694", "SRX13877715", "SRS11747887", "SRP356388", "PRJNA799842", "RNA seq of CRISPR/Cas9 mediated genome editing zebrafishes; the alligator pipefish spleen and the small white organ of the lined seahorse.", "PRJNA799842", "Other", "To explore the function of the gene in spleen development  we generated a knockout zebrafish line. To investigate whether the phenotypic consequences in the seahorses were caused by a missense mutation in this gene  we generated a point mutation zebrafish line by CRISPR/Cas9 mediated homologous recombination HR. RNA seq of these samples tissues brain  liver  kidney  and intestine were analyzed. In addition  to clarify the splenic phenotype of the Syngnathidaes  the transcriptomic profiles of the S. biaculeatus spleens and the H. erectus small white organ were also sampled and sequenced.", null, null, null, null, "WT br 2", null, "strain:AB type|isolate:wild type|breed:WT br 2|age:3 month|dev stage:NA|sex:NA|tissue:brain|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA seq of zebrafish", "WT br 2", "WT br 2", "RNA seq", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP356388", null, "loader:fastq load.py", "Daniorerio_WT-br-2_good_1.fq.gz Daniorerio_WT-br-2_good_2.fq.gz", "fastq fastq", 6877930220.0, 23036331.0, "Daniorerio WT br 2 good 1.fq.gz", "0:149.28 1:149.28", "A:1905660754;C:1522223390;G:1554569711;T:1895137309;N:339056", 149, 149, null, null, 1905660754, 1522223390, 1554569711, 1895137309, 339056, "SRX13877715", "SRS11747887", "SRA1360632", "South China Sea Institute of Oceanology|CAS Key Laboratory of Tropical Marine Bio-Resource", "South China Sea Institute of Oceanology", 2, 0.9194, 0.919, 0.17062, 0.16948, 0.69408, 0.69501, 0.49041, 0.46872, 101, 101, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2022-01-27", "Adult", "Adult", "Brain", "Nervous System"], [68352, "SRR17714695", "SRX13877714", "SRS11747888", "SRP356388", "PRJNA799842", "RNA seq of CRISPR/Cas9 mediated genome editing zebrafishes; the alligator pipefish spleen and the small white organ of the lined seahorse.", "PRJNA799842", "Other", "To explore the function of the gene in spleen development  we generated a knockout zebrafish line. To investigate whether the phenotypic consequences in the seahorses were caused by a missense mutation in this gene  we generated a point mutation zebrafish line by CRISPR/Cas9 mediated homologous recombination HR. RNA seq of these samples tissues brain  liver  kidney  and intestine were analyzed. In addition  to clarify the splenic phenotype of the Syngnathidaes  the transcriptomic profiles of the S. biaculeatus spleens and the H. erectus small white organ were also sampled and sequenced.", null, null, null, null, "WT br 1", null, "strain:AB type|isolate:wild type|breed:WT br 1|age:3 month|dev stage:NA|sex:NA|tissue:brain|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA seq of zebrafish", "WT br 1", "WT br 1", "RNA seq", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP356388", null, "loader:fastq load.py", "Daniorerio_WT-br-1_good_1.fq.gz Daniorerio_WT-br-1_good_2.fq.gz", "fastq fastq", 6677275476.0, 22349958.0, "Daniorerio WT br 1 good 1.fq.gz", "0:149.38 1:149.38", "A:1851526469;C:1477465442;G:1505977213;T:1841977322;N:329030", 149, 149, null, null, 1851526469, 1477465442, 1505977213, 1841977322, 329030, "SRX13877714", "SRS11747888", "SRA1360632", "South China Sea Institute of Oceanology|CAS Key Laboratory of Tropical Marine Bio-Resource", "South China Sea Institute of Oceanology", 2, 0.91982, 0.9189, 0.16897, 0.16834, 0.69631, 0.69802, 0.48999, 0.49186, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2022-01-27", "Adult", "Adult", "Brain", "Nervous System"], [68355, "SRR17714698", "SRX13877711", "SRS11747883", "SRP356388", "PRJNA799842", "RNA seq of CRISPR/Cas9 mediated genome editing zebrafishes; the alligator pipefish spleen and the small white organ of the lined seahorse.", "PRJNA799842", "Other", "To explore the function of the gene in spleen development  we generated a knockout zebrafish line. To investigate whether the phenotypic consequences in the seahorses were caused by a missense mutation in this gene  we generated a point mutation zebrafish line by CRISPR/Cas9 mediated homologous recombination HR. RNA seq of these samples tissues brain  liver  kidney  and intestine were analyzed. In addition  to clarify the splenic phenotype of the Syngnathidaes  the transcriptomic profiles of the S. biaculeatus spleens and the H. erectus small white organ were also sampled and sequenced.", null, null, null, null, "KO br 4", null, "strain:AB type|isolate:knockout|breed:KO br 4|age:3 month|dev stage:NA|sex:NA|tissue:brain|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA seq of zebrafish", "KO br 4", "KO br 4", "RNA seq", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP356388", null, "loader:fastq load.py", "Daniorerio_KO-br-4_good_1.fq.gz Daniorerio_KO-br-4_good_2.fq.gz", "fastq fastq", 7447389410.0, 24933883.0, "Daniorerio KO br 4 good 1.fq.gz", "0:149.34 1:149.34", "A:2068669098;C:1645485987;G:1683682550;T:2049528614;N:23161", 149, 149, null, null, 2068669098, 1645485987, 1683682550, 2049528614, 23161, "SRX13877711", "SRS11747883", "SRA1360632", "South China Sea Institute of Oceanology|CAS Key Laboratory of Tropical Marine Bio-Resource", "South China Sea Institute of Oceanology", 2, 0.91528, 0.91474, 0.16702, 0.16723, 0.69004, 0.69132, 0.48334, 0.48557, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2022-01-27", "Adult", "Adult", "Brain", "Nervous System"], [68366, "SRR17714709", "SRX13877700", "SRS11747872", "SRP356388", "PRJNA799842", "RNA seq of CRISPR/Cas9 mediated genome editing zebrafishes; the alligator pipefish spleen and the small white organ of the lined seahorse.", "PRJNA799842", "Other", "To explore the function of the gene in spleen development  we generated a knockout zebrafish line. To investigate whether the phenotypic consequences in the seahorses were caused by a missense mutation in this gene  we generated a point mutation zebrafish line by CRISPR/Cas9 mediated homologous recombination HR. RNA seq of these samples tissues brain  liver  kidney  and intestine were analyzed. In addition  to clarify the splenic phenotype of the Syngnathidaes  the transcriptomic profiles of the S. biaculeatus spleens and the H. erectus small white organ were also sampled and sequenced.", null, null, null, null, "KO br 3", null, "strain:AB type|isolate:knockout|breed:KO br 3|age:3 month|dev stage:NA|sex:NA|tissue:brain|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA seq of zebrafish", "KO br 3", "KO br 3", "RNA seq", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP356388", null, "loader:fastq load.py", "Daniorerio_KO-br-3_good_1.fq.gz Daniorerio_KO-br-3_good_2.fq.gz", "fastq fastq", 7677925588.0, 25687375.0, "Daniorerio KO br 3 good 1.fq.gz", "0:149.45 1:149.45", "A:2141293019;C:1686478627;G:1724464315;T:2125665018;N:24609", 149, 149, null, null, 2141293019, 1686478627, 1724464315, 2125665018, 24609, "SRX13877700", "SRS11747872", "SRA1360632", "South China Sea Institute of Oceanology|CAS Key Laboratory of Tropical Marine Bio-Resource", "South China Sea Institute of Oceanology", 2, 0.9154, 0.91488, 0.17232, 0.17035, 0.69227, 0.69449, 0.47379, 0.48628, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2022-01-27", "Adult", "Adult", "Brain", "Nervous System"], [68367, "SRR17714710", "SRX13877699", "SRS11747873", "SRP356388", "PRJNA799842", "RNA seq of CRISPR/Cas9 mediated genome editing zebrafishes; the alligator pipefish spleen and the small white organ of the lined seahorse.", "PRJNA799842", "Other", "To explore the function of the gene in spleen development  we generated a knockout zebrafish line. To investigate whether the phenotypic consequences in the seahorses were caused by a missense mutation in this gene  we generated a point mutation zebrafish line by CRISPR/Cas9 mediated homologous recombination HR. RNA seq of these samples tissues brain  liver  kidney  and intestine were analyzed. In addition  to clarify the splenic phenotype of the Syngnathidaes  the transcriptomic profiles of the S. biaculeatus spleens and the H. erectus small white organ were also sampled and sequenced.", null, null, null, null, "MU br 4", null, "strain:AB type|isolate:point mutation|breed:MU br 4|age:3 month|dev stage:NA|sex:NA|tissue:brain|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA seq of zebrafish", "MU br 4", "MU br 4", "RNA seq", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP356388", null, "loader:fastq load.py", "Daniorerio_MU-br-4_good_1.fq.gz Daniorerio_MU-br-4_good_2.fq.gz", "fastq fastq", 7375252226.0, 24699759.0, "Daniorerio MU br 4 good 1.fq.gz", "0:149.30 1:149.30", "A:2085033695;C:1591116585;G:1620970196;T:2077764269;N:367481", 149, 149, null, null, 2085033695, 1591116585, 1620970196, 2077764269, 367481, "SRX13877699", "SRS11747873", "SRA1360632", "South China Sea Institute of Oceanology|CAS Key Laboratory of Tropical Marine Bio-Resource", "South China Sea Institute of Oceanology", 2, 0.911, 0.90829, 0.19059, 0.18896, 0.6842, 0.68592, 0.49423, 0.49913, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2022-01-27", "Adult", "Adult", "Brain", "Nervous System"], [68368, "SRR17714711", "SRX13877698", "SRS11747871", "SRP356388", "PRJNA799842", "RNA seq of CRISPR/Cas9 mediated genome editing zebrafishes; the alligator pipefish spleen and the small white organ of the lined seahorse.", "PRJNA799842", "Other", "To explore the function of the gene in spleen development  we generated a knockout zebrafish line. To investigate whether the phenotypic consequences in the seahorses were caused by a missense mutation in this gene  we generated a point mutation zebrafish line by CRISPR/Cas9 mediated homologous recombination HR. RNA seq of these samples tissues brain  liver  kidney  and intestine were analyzed. In addition  to clarify the splenic phenotype of the Syngnathidaes  the transcriptomic profiles of the S. biaculeatus spleens and the H. erectus small white organ were also sampled and sequenced.", null, null, null, null, "MU br 3", null, "strain:AB type|isolate:point mutation|breed:MU br 3|age:3 month|dev stage:NA|sex:NA|tissue:brain|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA seq of zebrafish", "MU br 3", "MU br 3", "RNA seq", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP356388", null, "loader:fastq load.py", "Daniorerio_MU-br-3_good_1.fq.gz Daniorerio_MU-br-3_good_2.fq.gz", "fastq fastq", 8829806410.0, 29560399.0, "Daniorerio MU br 3 good 1.fq.gz", "0:149.35 1:149.35", "A:2496211455;C:1905517808;G:1942497707;T:2485137146;N:442294", 149, 149, null, null, 2496211455, 1905517808, 1942497707, 2485137146, 442294, "SRX13877698", "SRS11747871", "SRA1360632", "South China Sea Institute of Oceanology|CAS Key Laboratory of Tropical Marine Bio-Resource", "South China Sea Institute of Oceanology", 2, 0.91669, 0.91532, 0.19181, 0.19077, 0.69568, 0.69589, 0.48604, 0.48561, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2022-01-27", "Adult", "Adult", "Brain", "Nervous System"], [68369, "SRR17714712", "SRX13877697", "SRS11747869", "SRP356388", "PRJNA799842", "RNA seq of CRISPR/Cas9 mediated genome editing zebrafishes; the alligator pipefish spleen and the small white organ of the lined seahorse.", "PRJNA799842", "Other", "To explore the function of the gene in spleen development  we generated a knockout zebrafish line. To investigate whether the phenotypic consequences in the seahorses were caused by a missense mutation in this gene  we generated a point mutation zebrafish line by CRISPR/Cas9 mediated homologous recombination HR. RNA seq of these samples tissues brain  liver  kidney  and intestine were analyzed. In addition  to clarify the splenic phenotype of the Syngnathidaes  the transcriptomic profiles of the S. biaculeatus spleens and the H. erectus small white organ were also sampled and sequenced.", null, null, null, null, "MU br 2", null, "strain:AB type|isolate:point mutation|breed:MU br 2|age:3 month|dev stage:NA|sex:NA|tissue:brain|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA seq of zebrafish", "MU br 2", "MU br 2", "RNA seq", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP356388", null, "loader:fastq load.py", "Daniorerio_MU-br-2_good_1.fq.gz Daniorerio_MU-br-2_good_2.fq.gz", "fastq fastq", 7338057954.0, 24598168.0, "Daniorerio MU br 2 good 1.fq.gz", "0:149.16 1:149.16", "A:2058068771;C:1600708765;G:1631740601;T:2047172313;N:367504", 149, 149, null, null, 2058068771, 1600708765, 1631740601, 2047172313, 367504, "SRX13877697", "SRS11747869", "SRA1360632", "South China Sea Institute of Oceanology|CAS Key Laboratory of Tropical Marine Bio-Resource", "South China Sea Institute of Oceanology", 2, 0.91566, 0.91378, 0.18522, 0.18394, 0.69201, 0.69236, 0.4969, 0.49242, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2022-01-27", "Adult", "Adult", "Brain", "Nervous System"], [68370, "SRR17714713", "SRX13877696", "SRS11747870", "SRP356388", "PRJNA799842", "RNA seq of CRISPR/Cas9 mediated genome editing zebrafishes; the alligator pipefish spleen and the small white organ of the lined seahorse.", "PRJNA799842", "Other", "To explore the function of the gene in spleen development  we generated a knockout zebrafish line. To investigate whether the phenotypic consequences in the seahorses were caused by a missense mutation in this gene  we generated a point mutation zebrafish line by CRISPR/Cas9 mediated homologous recombination HR. RNA seq of these samples tissues brain  liver  kidney  and intestine were analyzed. In addition  to clarify the splenic phenotype of the Syngnathidaes  the transcriptomic profiles of the S. biaculeatus spleens and the H. erectus small white organ were also sampled and sequenced.", null, null, null, null, "MU br 1", null, "strain:AB type|isolate:point mutation|breed:MU br 1|age:3 month|dev stage:NA|sex:NA|tissue:brain|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA seq of zebrafish", "MU br 1", "MU br 1", "RNA seq", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP356388", null, "loader:fastq load.py", "Daniorerio_MU-br-1_good_1.fq.gz Daniorerio_MU-br-1_good_2.fq.gz", "fastq fastq", 8217544426.0, 27571297.0, "Daniorerio MU br 1 good 1.fq.gz", "0:149.02 1:149.02", "A:2292077990;C:1805071065;G:1840323909;T:2279661276;N:410186", 149, 149, null, null, 2292077990, 1805071065, 1840323909, 2279661276, 410186, "SRX13877696", "SRS11747870", "SRA1360632", "South China Sea Institute of Oceanology|CAS Key Laboratory of Tropical Marine Bio-Resource", "South China Sea Institute of Oceanology", 2, 0.91869, 0.91663, 0.1747, 0.17413, 0.69369, 0.69479, 0.46987, 0.47898, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2022-01-27", "Adult", "Adult", "Brain", "Nervous System"], [68377, "SRR17714720", "SRX13877689", "SRS11747861", "SRP356388", "PRJNA799842", "RNA seq of CRISPR/Cas9 mediated genome editing zebrafishes; the alligator pipefish spleen and the small white organ of the lined seahorse.", "PRJNA799842", "Other", "To explore the function of the gene in spleen development  we generated a knockout zebrafish line. To investigate whether the phenotypic consequences in the seahorses were caused by a missense mutation in this gene  we generated a point mutation zebrafish line by CRISPR/Cas9 mediated homologous recombination HR. RNA seq of these samples tissues brain  liver  kidney  and intestine were analyzed. In addition  to clarify the splenic phenotype of the Syngnathidaes  the transcriptomic profiles of the S. biaculeatus spleens and the H. erectus small white organ were also sampled and sequenced.", null, null, null, null, "KO br 2", null, "strain:AB type|isolate:knockout|breed:KO br 2|age:3 month|dev stage:NA|sex:NA|tissue:brain|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA seq of zebrafish", "KO br 2", "KO br 2", "RNA seq", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP356388", null, "loader:fastq load.py", "Daniorerio_KO-br-2_good_1.fq.gz Daniorerio_KO-br-2_good_2.fq.gz", "fastq fastq", 6926366374.0, 23168782.0, "Daniorerio KO br 2 good 1.fq.gz", "0:149.48 1:149.48", "A:1935071993;C:1518964140;G:1551567622;T:1920740127;N:22492", 149, 149, null, null, 1935071993, 1518964140, 1551567622, 1920740127, 22492, "SRX13877689", "SRS11747861", "SRA1360632", "South China Sea Institute of Oceanology|CAS Key Laboratory of Tropical Marine Bio-Resource", "South China Sea Institute of Oceanology", 2, 0.91501, 0.91449, 0.16712, 0.16608, 0.68927, 0.68974, 0.49324, 0.4917, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2022-01-27", "Adult", "Adult", "Brain", "Nervous System"], [68378, "SRR17714721", "SRX13877688", "SRS11747862", "SRP356388", "PRJNA799842", "RNA seq of CRISPR/Cas9 mediated genome editing zebrafishes; the alligator pipefish spleen and the small white organ of the lined seahorse.", "PRJNA799842", "Other", "To explore the function of the gene in spleen development  we generated a knockout zebrafish line. To investigate whether the phenotypic consequences in the seahorses were caused by a missense mutation in this gene  we generated a point mutation zebrafish line by CRISPR/Cas9 mediated homologous recombination HR. RNA seq of these samples tissues brain  liver  kidney  and intestine were analyzed. In addition  to clarify the splenic phenotype of the Syngnathidaes  the transcriptomic profiles of the S. biaculeatus spleens and the H. erectus small white organ were also sampled and sequenced.", null, null, null, null, "KO br 1", null, "strain:AB type|isolate:knockout|breed:KO br 1|age:3 month|dev stage:NA|sex:NA|tissue:brain|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA seq of zebrafish", "KO br 1", "KO br 1", "RNA seq", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina HiSeq 2500", null, "SRP356388", null, "loader:fastq load.py", "Daniorerio_KO-br-1_good_1.fq.gz Daniorerio_KO-br-1_good_2.fq.gz", "fastq fastq", 8695204456.0, 29088179.0, "Daniorerio KO br 1 good 1.fq.gz", "0:149.46 1:149.46", "A:2427675374;C:1907757731;G:1949179978;T:2410564055;N:27318", 149, 149, null, null, 2427675374, 1907757731, 1949179978, 2410564055, 27318, "SRX13877688", "SRS11747862", "SRA1360632", "South China Sea Institute of Oceanology|CAS Key Laboratory of Tropical Marine Bio-Resource", "South China Sea Institute of Oceanology", 2, 0.9149, 0.91459, 0.16735, 0.1673, 0.68954, 0.69023, 0.49497, 0.49633, 116, 116, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2022-01-27", "Adult", "Adult", "Brain", "Nervous System"], [70109, "SRR19561929", "SRX15614021", "SRS13314715", "SRP378802", "PRJNA846110", "Danio rerio Raw sequence reads", "PRJNA846110", "Other", "Temperature dependence of RNA editing in six tissues of the zebrafish Danio rerio", null, null, null, "Dr13 3", "Dr13 3", null, "strain:not applicable|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:not applicable|dev stage:adult|sex:female|tissue:multi tissue|aliquot:13 replicate 3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio: adult female 54 13 3 Brain", "54 13 3 Brain", "54 13 3 Brain", "All strand specific RNA seq libraries were constructed using VAHTS Stranded mRNA seq library Prep kit for Illumina v2  Vazyme Biotech Co.  Ltd  NR612 02  and subjected to 150bp paired end sequencing on a Novaseq 6053 illumina platform.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP378802", null, null, "54-13-3-Brain-RNA_R1.fq.gz 54-13-3-Brain-RNA_R2.fq.gz", "fastq fastq", 8519592000.0, 28398640.0, "54 13 3 Brain RNA R1.fq.gz", "0:150 1:150", "A:2380407110;C:1852161157;G:1886481748;T:2400379887;N:162098", 150, 150, null, null, 2380407110, 1852161157, 1886481748, 2400379887, 162098, "SRX15614021", "SRS13314715", "SRA1432943", "shanghai ocean university|College of Fisheries and Life Science", "shanghai ocean university", 2, 0.92362, 0.924, 0.15682, 0.15728, 0.70926, 0.71394, 0.49285, 0.50156, 150, 150, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2022-06-07", "Adult", "Adult", "Brain", "Nervous System"], [70113, "SRR19561933", "SRX15614017", "SRS13314707", "SRP378802", "PRJNA846110", "Danio rerio Raw sequence reads", "PRJNA846110", "Other", "Temperature dependence of RNA editing in six tissues of the zebrafish Danio rerio", null, null, null, "Dr27 1", "Dr27 1", null, "strain:not applicable|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:not applicable|dev stage:adult|sex:female|tissue:multi tissue|aliquot:27 replicate 1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio: adult female 46 27 1 Brain", "46 27 1 Brain", "46 27 1 Brain", "All strand specific RNA seq libraries were constructed using VAHTS Stranded mRNA seq library Prep kit for Illumina v2  Vazyme Biotech Co.  Ltd  NR612 02  and subjected to 150bp paired end sequencing on a Novaseq 6005 illumina platform.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP378802", null, null, "46-27-1-Brain-RNA_R1.fq.gz 46-27-1-Brain-RNA_R2.fq.gz", "fastq fastq", 6987525300.0, 23291751.0, "46 27 1 Brain RNA R1.fq.gz", "0:150 1:150", "A:2005854815;C:1466721021;G:1498208671;T:2016609597;N:131196", 150, 150, null, null, 2005854815, 1466721021, 1498208671, 2016609597, 131196, "SRX15614017", "SRS13314707", "SRA1432943", "shanghai ocean university|College of Fisheries and Life Science", "shanghai ocean university", 2, 0.9217, 0.92302, 0.16019, 0.15936, 0.7218, 0.72665, 0.51402, 0.49955, 150, 150, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2022-06-07", "Adult", "Adult", "Brain", "Nervous System"], [70116, "SRR19561936", "SRX15614014", "SRS13314714", "SRP378802", "PRJNA846110", "Danio rerio Raw sequence reads", "PRJNA846110", "Other", "Temperature dependence of RNA editing in six tissues of the zebrafish Danio rerio", null, null, null, "Dr13 2", "Dr13 2", null, "strain:not applicable|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:not applicable|dev stage:adult|sex:female|tissue:multi tissue|aliquot:13 replicate 2|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio: adult female 53 13 2 Brain", "53 13 2 Brain", "53 13 2 Brain", "All strand specific RNA seq libraries were constructed using VAHTS Stranded mRNA seq library Prep kit for Illumina v2  Vazyme Biotech Co.  Ltd  NR612 02  and subjected to 150bp paired end sequencing on a Novaseq 6047 illumina platform.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP378802", null, null, "53-13-2-Brain-RNA_R1.fq.gz 53-13-2-Brain-RNA_R2.fq.gz", "fastq fastq", 7058502300.0, 23528341.0, "53 13 2 Brain RNA R1.fq.gz", "0:150 1:150", "A:1992967170;C:1514239722;G:1545290515;T:2005873325;N:131568", 150, 150, null, null, 1992967170, 1514239722, 1545290515, 2005873325, 131568, "SRX15614014", "SRS13314714", "SRA1432943", "shanghai ocean university|College of Fisheries and Life Science", "shanghai ocean university", 2, 0.92235, 0.92276, 0.16057, 0.16091, 0.71226, 0.71873, 0.49675, 0.49871, 150, 150, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2022-06-07", "Adult", "Adult", "Brain", "Nervous System"], [70122, "SRR19561942", "SRX15614008", "SRS13314713", "SRP378802", "PRJNA846110", "Danio rerio Raw sequence reads", "PRJNA846110", "Other", "Temperature dependence of RNA editing in six tissues of the zebrafish Danio rerio", null, null, null, "Dr13 1", "Dr13 1", null, "strain:not applicable|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:not applicable|dev stage:adult|sex:female|tissue:multi tissue|aliquot:13 replicate 1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio: adult female 52 13 1 Brain", "52 13 1 Brain", "52 13 1 Brain", "All strand specific RNA seq libraries were constructed using VAHTS Stranded mRNA seq library Prep kit for Illumina v2  Vazyme Biotech Co.  Ltd  NR612 02  and subjected to 150bp paired end sequencing on a Novaseq 6041 illumina platform.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP378802", null, null, "52-13-1-Brain-RNA_R1.fq.gz 52-13-1-Brain-RNA_R2.fq.gz", "fastq fastq", 6564342900.0, 21881143.0, "52 13 1 Brain RNA R1.fq.gz", "0:150 1:150", "A:1875036799;C:1388864833;G:1412506225;T:1887810961;N:124082", 150, 150, null, null, 1875036799, 1388864833, 1412506225, 1887810961, 124082, "SRX15614008", "SRS13314713", "SRA1432943", "shanghai ocean university|College of Fisheries and Life Science", "shanghai ocean university", 2, 0.92113, 0.9218, 0.16856, 0.16862, 0.71147, 0.71776, 0.48601, 0.50846, 150, 150, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2022-06-07", "Adult", "Adult", "Brain", "Nervous System"], [70129, "SRR19561949", "SRX15614001", "SRS13314712", "SRP378802", "PRJNA846110", "Danio rerio Raw sequence reads", "PRJNA846110", "Other", "Temperature dependence of RNA editing in six tissues of the zebrafish Danio rerio", null, null, null, "Dr18 3", "Dr18 3", null, "strain:not applicable|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:not applicable|dev stage:adult|sex:female|tissue:multi tissue|aliquot:18 replicate 3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio: adult female 51 18 3 Brain", "51 18 3 Brain", "51 18 3 Brain", "All strand specific RNA seq libraries were constructed using VAHTS Stranded mRNA seq library Prep kit for Illumina v2  Vazyme Biotech Co.  Ltd  NR612 02  and subjected to 150bp paired end sequencing on a Novaseq 6035 illumina platform.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP378802", null, null, "51-18-3-Brain-RNA_R1.fq.gz 51-18-3-Brain-RNA_R2.fq.gz", "fastq fastq", 6606239700.0, 22020799.0, "51 18 3 Brain RNA R1.fq.gz", "0:150 1:150", "A:1885996342;C:1397743068;G:1420407935;T:1901969274;N:123081", 150, 150, null, null, 1885996342, 1397743068, 1420407935, 1901969274, 123081, "SRX15614001", "SRS13314712", "SRA1432943", "shanghai ocean university|College of Fisheries and Life Science", "shanghai ocean university", 2, 0.92037, 0.92037, 0.17349, 0.17351, 0.7134, 0.71961, 0.49757, 0.5036, 150, 150, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2022-06-07", "Adult", "Adult", "Brain", "Nervous System"], [70136, "SRR19561956", "SRX15613994", "SRS13314711", "SRP378802", "PRJNA846110", "Danio rerio Raw sequence reads", "PRJNA846110", "Other", "Temperature dependence of RNA editing in six tissues of the zebrafish Danio rerio", null, null, null, "Dr18 2", "Dr18 2", null, "strain:not applicable|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:not applicable|dev stage:adult|sex:female|tissue:multi tissue|aliquot:18 replicate 2|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio: adult female 50 18 2 Brain", "50 18 2 Brain", "50 18 2 Brain", "All strand specific RNA seq libraries were constructed using VAHTS Stranded mRNA seq library Prep kit for Illumina v2  Vazyme Biotech Co.  Ltd  NR612 02  and subjected to 150bp paired end sequencing on a Novaseq 6029 illumina platform.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP378802", null, null, "50-18-2-Brain-RNA_R1.fq.gz 50-18-2-Brain-RNA_R2.fq.gz", "fastq fastq", 6281424600.0, 20938082.0, "50 18 2 Brain RNA R1.fq.gz", "0:150 1:150", "A:1790409321;C:1331778838;G:1355326334;T:1803791514;N:118593", 150, 150, null, null, 1790409321, 1331778838, 1355326334, 1803791514, 118593, "SRX15613994", "SRS13314711", "SRA1432943", "shanghai ocean university|College of Fisheries and Life Science", "shanghai ocean university", 2, 0.92329, 0.92423, 0.16356, 0.16369, 0.71788, 0.72366, 0.50986, 0.51265, 150, 150, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2022-06-07", "Adult", "Adult", "Brain", "Nervous System"], [70142, "SRR19561962", "SRX15613988", "SRS13314710", "SRP378802", "PRJNA846110", "Danio rerio Raw sequence reads", "PRJNA846110", "Other", "Temperature dependence of RNA editing in six tissues of the zebrafish Danio rerio", null, null, null, "Dr18 1", "Dr18 1", null, "strain:not applicable|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:not applicable|dev stage:adult|sex:female|tissue:multi tissue|aliquot:18 replicate 1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio: adult female 49 18 1 Brain", "49 18 1 Brain", "49 18 1 Brain", "All strand specific RNA seq libraries were constructed using VAHTS Stranded mRNA seq library Prep kit for Illumina v2  Vazyme Biotech Co.  Ltd  NR612 02  and subjected to 150bp paired end sequencing on a Novaseq 6023 illumina platform.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP378802", null, null, "49-18-1-Brain-RNA_R1.fq.gz 49-18-1-Brain-RNA_R2.fq.gz", "fastq fastq", 7316116800.0, 24387056.0, "49 18 1 Brain RNA R1.fq.gz", "0:150 1:150", "A:2029219672;C:1605053166;G:1629415583;T:2052291110;N:137269", 150, 150, null, null, 2029219672, 1605053166, 1629415583, 2052291110, 137269, "SRX15613988", "SRS13314710", "SRA1432943", "shanghai ocean university|College of Fisheries and Life Science", "shanghai ocean university", 2, 0.92845, 0.92831, 0.15339, 0.15343, 0.70163, 0.70818, 0.50561, 0.49113, 150, 150, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2022-06-07", "Adult", "Adult", "Brain", "Nervous System"], [70149, "SRR19561969", "SRX15613981", "SRS13314709", "SRP378802", "PRJNA846110", "Danio rerio Raw sequence reads", "PRJNA846110", "Other", "Temperature dependence of RNA editing in six tissues of the zebrafish Danio rerio", null, null, null, "Dr27 3", "Dr27 3", null, "strain:not applicable|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:not applicable|dev stage:adult|sex:female|tissue:multi tissue|aliquot:27 replicate 3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio: adult female 48 27 3 Brain", "48 27 3 Brain", "48 27 3 Brain", "All strand specific RNA seq libraries were constructed using VAHTS Stranded mRNA seq library Prep kit for Illumina v2  Vazyme Biotech Co.  Ltd  NR612 02  and subjected to 150bp paired end sequencing on a Novaseq 6017 illumina platform.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP378802", null, null, "48-27-3-Brain-RNA_R1.fq.gz 48-27-3-Brain-RNA_R2.fq.gz", "fastq fastq", 7425268800.0, 24750896.0, "48 27 3 Brain RNA R1.fq.gz", "0:150 1:150", "A:2180771736;C:1512927753;G:1541462587;T:2189964944;N:141780", 150, 150, null, null, 2180771736, 1512927753, 1541462587, 2189964944, 141780, "SRX15613981", "SRS13314709", "SRA1432943", "shanghai ocean university|College of Fisheries and Life Science", "shanghai ocean university", 2, 0.92505, 0.92554, 0.17225, 0.17055, 0.73162, 0.73634, 0.54078, 0.54258, 150, 150, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2022-06-07", "Adult", "Adult", "Brain", "Nervous System"], [70155, "SRR19561975", "SRX15613975", "SRS13314708", "SRP378802", "PRJNA846110", "Danio rerio Raw sequence reads", "PRJNA846110", "Other", "Temperature dependence of RNA editing in six tissues of the zebrafish Danio rerio", null, null, null, "Dr27 2", "Dr27 2", null, "strain:not applicable|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:not applicable|dev stage:adult|sex:female|tissue:multi tissue|aliquot:27 replicate 2|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of Danio rerio: adult female 47 27 2 Brain", "47 27 2 Brain", "47 27 2 Brain", "All strand specific RNA seq libraries were constructed using VAHTS Stranded mRNA seq library Prep kit for Illumina v2  Vazyme Biotech Co.  Ltd  NR612 02  and subjected to 150bp paired end sequencing on a Novaseq 6011 illumina platform.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP378802", null, null, "47-27-2-Brain-RNA_R1.fq.gz 47-27-2-Brain-RNA_R2.fq.gz", "fastq fastq", 8266981800.0, 27556606.0, "47 27 2 Brain RNA R1.fq.gz", "0:150 1:150", "A:2439238017;C:1674208335;G:1753393244;T:2400007685;N:134519", 150, 150, null, null, 2439238017, 1674208335, 1753393244, 2400007685, 134519, "SRX15613975", "SRS13314708", "SRA1432943", "shanghai ocean university|College of Fisheries and Life Science", "shanghai ocean university", 2, 0.93567, 0.91255, 0.15677, 0.15133, 0.73728, 0.74059, 0.58353, 0.5211, 150, 150, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "China", "2022-06-07", "Adult", "Adult", "Brain", "Nervous System"], [75356, "SRR24489425", "SRX20274795", "SRS17602489", "SRP436765", "PRJNA970658", "Transcriptome analysis of Cygb1 Knockout in Zebrafish Danio rerio", "PRJNA970658", "Other", "Cytoglobin Cygb is an evolutionary ancient heme protein with yet unclear physiological functions. Cygb might be involved in ROS detoxification  NO metabolism and lipid based signaling processes. Mammalian Cygb is ubiquitously expressed in all tissues. We used a CRISPR/Cas9 genome editing approach and generated a knockout of the penta coordinated cygb1 for in vivo analysis in zebrafish. Adult cygb1 knockouts develop phenotypic anomalies  including neoplasia of gonads  and weight loss. To identify the molecular mechanisms underlying the occurrence of these phenotypes and differentiate between function and effect of the knockout we compared the transcriptomes of cygb1 knockouts at different ages to age matched wild type WT zebrafish.", null, null, null, "Adult Brain F5", "F5 WT brain N R1 001", null, "strain:Tuebingen|isolate:WT Brain F5|age:12 mpf|dev stage:adult|sex:male|tissue:brain|genotype:wildtype|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNAseq Zebrafish 12mpf Brain WT", "F5 WT brain N", "F5 WT brain N", "Total RNA was extracted using the Crystal RNA Mini Kit following the instructions of the manufacturer. RNA samples were treated with DNaseI Qiagen  according to the manufacturers protocol. RNA concentration and quality for Illumina sequencing was measured by employing the TapeStation System 2200. A library for paired end sequencing was generated from 1 g total RNA.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina HiSeq 4000", null, "SRP436765", null, null, "F5-WT-brain-N_R1_001.fastq.gz F5-WT-brain-N_R2_001.fastq.gz", "fastq fastq", 6841055400.0, 22803518.0, "F5 WT brain N R1 001.fastq.gz", "0:150 1:150", "A:1812654704;C:1604487806;G:1679235049;T:1744234054;N:443787", 150, 150, null, null, 1812654704, 1604487806, 1679235049, 1744234054, 443787, "SRX20274795", "SRS17602489", "SRA1634682", "University of Hamburg|Institute of Animal Cell and Systems Biology (IZS)", "University of Hamburg", 2, 0.93773, 0.93874, 0.10994, 0.10853, 0.69426, 0.69818, 0.51982, 0.52431, 150, 150, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "Germany", "2023-05-10", "Adult", "Adult", "Brain", "Nervous System"], [75362, "SRR24489431", "SRX20274789", "SRS17602483", "SRP436765", "PRJNA970658", "Transcriptome analysis of Cygb1 Knockout in Zebrafish Danio rerio", "PRJNA970658", "Other", "Cytoglobin Cygb is an evolutionary ancient heme protein with yet unclear physiological functions. Cygb might be involved in ROS detoxification  NO metabolism and lipid based signaling processes. Mammalian Cygb is ubiquitously expressed in all tissues. We used a CRISPR/Cas9 genome editing approach and generated a knockout of the penta coordinated cygb1 for in vivo analysis in zebrafish. Adult cygb1 knockouts develop phenotypic anomalies  including neoplasia of gonads  and weight loss. To identify the molecular mechanisms underlying the occurrence of these phenotypes and differentiate between function and effect of the knockout we compared the transcriptomes of cygb1 knockouts at different ages to age matched wild type WT zebrafish.", null, null, null, "Old KO Brain 6", "Cygb1KO G 6 S32 R1 001", null, "strain:Tuebingen|isolate:Brain 6 Cygb1KO|age:24 mpf|dev stage:adult|sex:male|tissue:brain|genotype:cygb1 knockout|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNAseq Zebrafish 24mpf Brain Cygb1KO", "Cygb1KO G 6S32", "Cygb1KO G 6S32", "Total RNA was extracted using the Crystal RNA Mini Kit following the instructions of the manufacturer. RNA samples were treated with DNaseI Qiagen  according to the manufacturers protocol. RNA concentration and quality for Illumina sequencing was measured by employing the TapeStation System 2200. A library for paired end sequencing was generated from 1 g total RNA.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP436765", null, null, "Cygb1KO-G-6_S32_R1_001.fastq.gz Cygb1KO-G-6_S32_R2_001.fastq.gz", "fastq fastq", 9925695180.0, 34149517.0, "Cygb1KO G 6 S32 R1 001.fastq.gz", "0:142.59 1:148.06", "A:2929412339;C:1950048888;G:2154536328;T:2880617061;N:11080564", 142, 148, null, null, 2929412339, 1950048888, 2154536328, 2880617061, 11080564, "SRX20274789", "SRS17602483", "SRA1634682", "University of Hamburg|Institute of Animal Cell and Systems Biology (IZS)", "University of Hamburg", 2, 0.9273, 0.92531, 0.25488, 0.2525, 0.71794, 0.71561, 0.53653, 0.53821, 149, 149, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "Germany", "2023-05-10", "Adult", "Adult", "Brain", "Nervous System"], [75364, "SRR24489433", "SRX20274787", "SRS17602478", "SRP436765", "PRJNA970658", "Transcriptome analysis of Cygb1 Knockout in Zebrafish Danio rerio", "PRJNA970658", "Other", "Cytoglobin Cygb is an evolutionary ancient heme protein with yet unclear physiological functions. Cygb might be involved in ROS detoxification  NO metabolism and lipid based signaling processes. Mammalian Cygb is ubiquitously expressed in all tissues. We used a CRISPR/Cas9 genome editing approach and generated a knockout of the penta coordinated cygb1 for in vivo analysis in zebrafish. Adult cygb1 knockouts develop phenotypic anomalies  including neoplasia of gonads  and weight loss. To identify the molecular mechanisms underlying the occurrence of these phenotypes and differentiate between function and effect of the knockout we compared the transcriptomes of cygb1 knockouts at different ages to age matched wild type WT zebrafish.", null, null, null, "Old KO Brain 4", "Cygb1KO G 4 S31 R1 001", null, "strain:Tuebingen|isolate:Brain 4 Cygb1KO|age:24 mpf|dev stage:adult|sex:male|tissue:brain|genotype:cygb1 knockout|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNAseq Zebrafish 24mpf Brain Cygb1KO", "Cygb1KO G 4S31", "Cygb1KO G 4S31", "Total RNA was extracted using the Crystal RNA Mini Kit following the instructions of the manufacturer. RNA samples were treated with DNaseI Qiagen  according to the manufacturers protocol. RNA concentration and quality for Illumina sequencing was measured by employing the TapeStation System 2200. A library for paired end sequencing was generated from 1 g total RNA.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP436765", null, null, "Cygb1KO-G-4_S31_R1_001.fastq.gz Cygb1KO-G-4_S31_R2_001.fastq.gz", "fastq fastq", 9815347477.0, 33708992.0, "Cygb1KO G 4 S31 R1 001.fastq.gz", "0:143.34 1:147.83", "A:2847426769;C:1996151135;G:2157346007;T:2805302254;N:9121312", 143, 147, null, null, 2847426769, 1996151135, 2157346007, 2805302254, 9121312, "SRX20274787", "SRS17602478", "SRA1634682", "University of Hamburg|Institute of Animal Cell and Systems Biology (IZS)", "University of Hamburg", 2, 0.93535, 0.93362, 0.22332, 0.21868, 0.70786, 0.70579, 0.51367, 0.50733, 150, 150, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "Germany", "2023-05-10", "Adult", "Adult", "Brain", "Nervous System"], [75366, "SRR24489435", "SRX20274785", "SRS17602477", "SRP436765", "PRJNA970658", "Transcriptome analysis of Cygb1 Knockout in Zebrafish Danio rerio", "PRJNA970658", "Other", "Cytoglobin Cygb is an evolutionary ancient heme protein with yet unclear physiological functions. Cygb might be involved in ROS detoxification  NO metabolism and lipid based signaling processes. Mammalian Cygb is ubiquitously expressed in all tissues. We used a CRISPR/Cas9 genome editing approach and generated a knockout of the penta coordinated cygb1 for in vivo analysis in zebrafish. Adult cygb1 knockouts develop phenotypic anomalies  including neoplasia of gonads  and weight loss. To identify the molecular mechanisms underlying the occurrence of these phenotypes and differentiate between function and effect of the knockout we compared the transcriptomes of cygb1 knockouts at different ages to age matched wild type WT zebrafish.", null, null, null, "Old KO Brain 3", "Cygb1KO G 3 S30 R1 001", null, "strain:Tuebingen|isolate:Brain 3 Cygb1KO|age:24 mpf|dev stage:adult|sex:male|tissue:brain|genotype:cygb1 knockout|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNAseq Zebrafish 24mpf Brain Cygb1KO", "Cygb1KO G 3S30", "Cygb1KO G 3S30", "Total RNA was extracted using the Crystal RNA Mini Kit following the instructions of the manufacturer. RNA samples were treated with DNaseI Qiagen  according to the manufacturers protocol. RNA concentration and quality for Illumina sequencing was measured by employing the TapeStation System 2200. A library for paired end sequencing was generated from 1 g total RNA.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP436765", null, null, "Cygb1KO-G-3_S30_R1_001.fastq.gz Cygb1KO-G-3_S30_R2_001.fastq.gz", "fastq fastq", 6375806624.0, 22076571.0, "Cygb1KO G 3 S30 R1 001.fastq.gz", "0:140.65 1:148.15", "A:1838824763;C:1269837958;G:1450334143;T:1807137695;N:9672065", 140, 148, null, null, 1838824763, 1269837958, 1450334143, 1807137695, 9672065, "SRX20274785", "SRS17602477", "SRA1634682", "University of Hamburg|Institute of Animal Cell and Systems Biology (IZS)", "University of Hamburg", 2, 0.93667, 0.93108, 0.22667, 0.22219, 0.71131, 0.70883, 0.51796, 0.50211, 149, 149, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "Germany", "2023-05-10", "Adult", "Adult", "Brain", "Nervous System"], [75369, "SRR24489438", "SRX20274782", "SRS17602473", "SRP436765", "PRJNA970658", "Transcriptome analysis of Cygb1 Knockout in Zebrafish Danio rerio", "PRJNA970658", "Other", "Cytoglobin Cygb is an evolutionary ancient heme protein with yet unclear physiological functions. Cygb might be involved in ROS detoxification  NO metabolism and lipid based signaling processes. Mammalian Cygb is ubiquitously expressed in all tissues. We used a CRISPR/Cas9 genome editing approach and generated a knockout of the penta coordinated cygb1 for in vivo analysis in zebrafish. Adult cygb1 knockouts develop phenotypic anomalies  including neoplasia of gonads  and weight loss. To identify the molecular mechanisms underlying the occurrence of these phenotypes and differentiate between function and effect of the knockout we compared the transcriptomes of cygb1 knockouts at different ages to age matched wild type WT zebrafish.", null, null, null, "Old KO Brain 2", "Cygb1KO G 2 S29 R1 001", null, "strain:Tuebingen|isolate:Brain 2 Cygb1KO|age:24 mpf|dev stage:adult|sex:male|tissue:brain|genotype:cygb1 knockout|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNAseq Zebrafish 24mpf Brain Cygb1KO", "Cygb1KO G 2S29", "Cygb1KO G 2S29", "Total RNA was extracted using the Crystal RNA Mini Kit following the instructions of the manufacturer. RNA samples were treated with DNaseI Qiagen  according to the manufacturers protocol. RNA concentration and quality for Illumina sequencing was measured by employing the TapeStation System 2200. A library for paired end sequencing was generated from 1 g total RNA.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP436765", null, null, "Cygb1KO-G-2_S29_R1_001.fastq.gz Cygb1KO-G-2_S29_R2_001.fastq.gz", "fastq fastq", 9228083087.0, 31353095.0, "Cygb1KO G 2 S29 R1 001.fastq.gz", "0:146.81 1:147.51", "A:2685482962;C:1925153227;G:1961258559;T:2654447279;N:1741060", 146, 147, null, null, 2685482962, 1925153227, 1961258559, 2654447279, 1741060, "SRX20274782", "SRS17602473", "SRA1634682", "University of Hamburg|Institute of Animal Cell and Systems Biology (IZS)", "University of Hamburg", 2, 0.94076, 0.94161, 0.20564, 0.20278, 0.70498, 0.70157, 0.51187, 0.51437, 150, 150, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "Germany", "2023-05-10", "Adult", "Adult", "Brain", "Nervous System"], [75371, "SRR24489440", "SRX20274780", "SRS17602476", "SRP436765", "PRJNA970658", "Transcriptome analysis of Cygb1 Knockout in Zebrafish Danio rerio", "PRJNA970658", "Other", "Cytoglobin Cygb is an evolutionary ancient heme protein with yet unclear physiological functions. Cygb might be involved in ROS detoxification  NO metabolism and lipid based signaling processes. Mammalian Cygb is ubiquitously expressed in all tissues. We used a CRISPR/Cas9 genome editing approach and generated a knockout of the penta coordinated cygb1 for in vivo analysis in zebrafish. Adult cygb1 knockouts develop phenotypic anomalies  including neoplasia of gonads  and weight loss. To identify the molecular mechanisms underlying the occurrence of these phenotypes and differentiate between function and effect of the knockout we compared the transcriptomes of cygb1 knockouts at different ages to age matched wild type WT zebrafish.", null, null, null, "Old Brain 4", "4 brainWTm R1 001", null, "strain:Tuebingen|isolate:Old Brain 4|age:24 mpf|dev stage:adult|sex:male|tissue:brain|genotype:wildtype|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNAseq Zebrafish 24mpf Brain WT", "4 brainWTm", "4 brainWTm", "Total RNA was extracted using the Crystal RNA Mini Kit following the instructions of the manufacturer. RNA samples were treated with DNaseI Qiagen  according to the manufacturers protocol. RNA concentration and quality for Illumina sequencing was measured by employing the TapeStation System 2200. A library for paired end sequencing was generated from 1 g total RNA.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP436765", null, null, "4-brainWTm_R1_001.fastq.gz 4-brainWTm_R2_001.fastq.gz", "fastq fastq", 5391512414.0, 18695220.0, "4 brainWTm R1 001.fastq.gz", "0:143.36 1:145.03", "A:1526986732;C:1144838870;G:1197115002;T:1511509189;N:11062621", 143, 145, null, null, 1526986732, 1144838870, 1197115002, 1511509189, 11062621, "SRX20274780", "SRS17602476", "SRA1634682", "University of Hamburg|Institute of Animal Cell and Systems Biology (IZS)", "University of Hamburg", 2, 0.93479, 0.93458, 0.18008, 0.17489, 0.70652, 0.70772, 0.52976, 0.52891, 150, 149, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "Germany", "2023-05-10", "Adult", "Adult", "Brain", "Nervous System"], [75373, "SRR24489442", "SRX20274778", "SRS17602472", "SRP436765", "PRJNA970658", "Transcriptome analysis of Cygb1 Knockout in Zebrafish Danio rerio", "PRJNA970658", "Other", "Cytoglobin Cygb is an evolutionary ancient heme protein with yet unclear physiological functions. Cygb might be involved in ROS detoxification  NO metabolism and lipid based signaling processes. Mammalian Cygb is ubiquitously expressed in all tissues. We used a CRISPR/Cas9 genome editing approach and generated a knockout of the penta coordinated cygb1 for in vivo analysis in zebrafish. Adult cygb1 knockouts develop phenotypic anomalies  including neoplasia of gonads  and weight loss. To identify the molecular mechanisms underlying the occurrence of these phenotypes and differentiate between function and effect of the knockout we compared the transcriptomes of cygb1 knockouts at different ages to age matched wild type WT zebrafish.", null, null, null, "Old Brain 3", "3 brainWTm R1 001", null, "strain:Tuebingen|isolate:Old Brain 3|age:24 mpf|dev stage:adult|sex:male|tissue:brain|genotype:wildtype|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNAseq Zebrafish 24mpf Brain WT", "3 brainWTm", "3 brainWTm", "Total RNA was extracted using the Crystal RNA Mini Kit following the instructions of the manufacturer. RNA samples were treated with DNaseI Qiagen  according to the manufacturers protocol. RNA concentration and quality for Illumina sequencing was measured by employing the TapeStation System 2200. A library for paired end sequencing was generated from 1 g total RNA.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP436765", null, null, "3-brainWTm_R1_001.fastq.gz 3-brainWTm_R2_001.fastq.gz", "fastq fastq", 7193574753.0, 25032649.0, "3 brainWTm R1 001.fastq.gz", "0:143.01 1:144.35", "A:2045307156;C:1525864162;G:1582858563;T:2027473626;N:12071246", 143, 144, null, null, 2045307156, 1525864162, 1582858563, 2027473626, 12071246, "SRX20274778", "SRS17602472", "SRA1634682", "University of Hamburg|Institute of Animal Cell and Systems Biology (IZS)", "University of Hamburg", 2, 0.93262, 0.93305, 0.18864, 0.18297, 0.70522, 0.70755, 0.52309, 0.50982, 149, 150, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "Germany", "2023-05-10", "Adult", "Adult", "Brain", "Nervous System"], [75375, "SRR24489444", "SRX20274776", "SRS17602470", "SRP436765", "PRJNA970658", "Transcriptome analysis of Cygb1 Knockout in Zebrafish Danio rerio", "PRJNA970658", "Other", "Cytoglobin Cygb is an evolutionary ancient heme protein with yet unclear physiological functions. Cygb might be involved in ROS detoxification  NO metabolism and lipid based signaling processes. Mammalian Cygb is ubiquitously expressed in all tissues. We used a CRISPR/Cas9 genome editing approach and generated a knockout of the penta coordinated cygb1 for in vivo analysis in zebrafish. Adult cygb1 knockouts develop phenotypic anomalies  including neoplasia of gonads  and weight loss. To identify the molecular mechanisms underlying the occurrence of these phenotypes and differentiate between function and effect of the knockout we compared the transcriptomes of cygb1 knockouts at different ages to age matched wild type WT zebrafish.", null, null, null, "Old Brain 2", "2 brainWTm R1 001", null, "strain:Tuebingen|isolate:Old Brain 2|age:24 mpf|dev stage:adult|sex:male|tissue:brain|genotype:wildtype|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNAseq Zebrafish 24mpf Brain WT", "2 brainWTm", "2 brainWTm", "Total RNA was extracted using the Crystal RNA Mini Kit following the instructions of the manufacturer. RNA samples were treated with DNaseI Qiagen  according to the manufacturers protocol. RNA concentration and quality for Illumina sequencing was measured by employing the TapeStation System 2200. A library for paired end sequencing was generated from 1 g total RNA.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP436765", null, null, "2-brainWTm_R1_001.fastq.gz 2-brainWTm_R2_001.fastq.gz", "fastq fastq", 6187362464.0, 21465519.0, "2 brainWTm R1 001.fastq.gz", "0:143.41 1:144.83", "A:1748094759;C:1321274648;G:1378525698;T:1728446529;N:11020830", 143, 144, null, null, 1748094759, 1321274648, 1378525698, 1728446529, 11020830, "SRX20274776", "SRS17602470", "SRA1634682", "University of Hamburg|Institute of Animal Cell and Systems Biology (IZS)", "University of Hamburg", 2, 0.93634, 0.93569, 0.19002, 0.18498, 0.70583, 0.70912, 0.49674, 0.50861, 150, 150, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "Germany", "2023-05-10", "Adult", "Adult", "Brain", "Nervous System"], [75377, "SRR24489446", "SRX20274774", "SRS17602469", "SRP436765", "PRJNA970658", "Transcriptome analysis of Cygb1 Knockout in Zebrafish Danio rerio", "PRJNA970658", "Other", "Cytoglobin Cygb is an evolutionary ancient heme protein with yet unclear physiological functions. Cygb might be involved in ROS detoxification  NO metabolism and lipid based signaling processes. Mammalian Cygb is ubiquitously expressed in all tissues. We used a CRISPR/Cas9 genome editing approach and generated a knockout of the penta coordinated cygb1 for in vivo analysis in zebrafish. Adult cygb1 knockouts develop phenotypic anomalies  including neoplasia of gonads  and weight loss. To identify the molecular mechanisms underlying the occurrence of these phenotypes and differentiate between function and effect of the knockout we compared the transcriptomes of cygb1 knockouts at different ages to age matched wild type WT zebrafish.", null, null, null, "Old Brain 1", "1 brainWTm R1 001", null, "strain:Tuebingen|isolate:Old Brain 1|age:24 mpf|dev stage:adult|sex:male|tissue:brain|genotype:wildtype|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNAseq Zebrafish 24mpf Brain WT", "1 brainWTm", "1 brainWTm", "Total RNA was extracted using the Crystal RNA Mini Kit following the instructions of the manufacturer. RNA samples were treated with DNaseI Qiagen  according to the manufacturers protocol. RNA concentration and quality for Illumina sequencing was measured by employing the TapeStation System 2200. 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To identify the molecular mechanisms underlying the occurrence of these phenotypes and differentiate between function and effect of the knockout we compared the transcriptomes of cygb1 knockouts at different ages to age matched wild type WT zebrafish.", null, null, null, "Adult KO brain F8", "F8 KO brain N R1 001", null, "strain:Tuebingen|isolate:KO brain F8|age:12 mpf|dev stage:adult|sex:male|tissue:brain|genotype:cygb1 knockout|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNAseq Zebrafish 12mpf Brain Cygb1KO", "F8 KO brain N", "F8 KO brain N", "Total RNA was extracted using the Crystal RNA Mini Kit following the instructions of the manufacturer. RNA samples were treated with DNaseI Qiagen  according to the manufacturers protocol. RNA concentration and quality for Illumina sequencing was measured by employing the TapeStation System 2200. 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Cygb might be involved in ROS detoxification  NO metabolism and lipid based signaling processes. Mammalian Cygb is ubiquitously expressed in all tissues. We used a CRISPR/Cas9 genome editing approach and generated a knockout of the penta coordinated cygb1 for in vivo analysis in zebrafish. Adult cygb1 knockouts develop phenotypic anomalies  including neoplasia of gonads  and weight loss. To identify the molecular mechanisms underlying the occurrence of these phenotypes and differentiate between function and effect of the knockout we compared the transcriptomes of cygb1 knockouts at different ages to age matched wild type WT zebrafish.", null, null, null, "Adult KO brain F4", "F4 KO brain N R1 001", null, "strain:Tuebingen|isolate:KO brain F4|age:12 mpf|dev stage:adult|sex:male|tissue:brain|genotype:cygb1 knockout|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNAseq Zebrafish 12mpf Brain Cygb1KO", "F4 KO brain N", "F4 KO brain N", "Total RNA was extracted using the Crystal RNA Mini Kit following the instructions of the manufacturer. RNA samples were treated with DNaseI Qiagen  according to the manufacturers protocol. RNA concentration and quality for Illumina sequencing was measured by employing the TapeStation System 2200. 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Cygb might be involved in ROS detoxification  NO metabolism and lipid based signaling processes. Mammalian Cygb is ubiquitously expressed in all tissues. We used a CRISPR/Cas9 genome editing approach and generated a knockout of the penta coordinated cygb1 for in vivo analysis in zebrafish. Adult cygb1 knockouts develop phenotypic anomalies  including neoplasia of gonads  and weight loss. To identify the molecular mechanisms underlying the occurrence of these phenotypes and differentiate between function and effect of the knockout we compared the transcriptomes of cygb1 knockouts at different ages to age matched wild type WT zebrafish.", null, null, null, "Adult KO brain F1", "F1 KO brain N R1 001", null, "strain:Tuebingen|isolate:KO brain F1|age:12 mpf|dev stage:adult|sex:male|tissue:brain|genotype:cygb1 knockout|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNAseq Zebrafish 12mpf Brain Cygb1KO", "F1 KO brain N", "F1 KO brain N", "Total RNA was extracted using the Crystal RNA Mini Kit following the instructions of the manufacturer. RNA samples were treated with DNaseI Qiagen  according to the manufacturers protocol. RNA concentration and quality for Illumina sequencing was measured by employing the TapeStation System 2200. 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Cygb might be involved in ROS detoxification  NO metabolism and lipid based signaling processes. Mammalian Cygb is ubiquitously expressed in all tissues. We used a CRISPR/Cas9 genome editing approach and generated a knockout of the penta coordinated cygb1 for in vivo analysis in zebrafish. Adult cygb1 knockouts develop phenotypic anomalies  including neoplasia of gonads  and weight loss. To identify the molecular mechanisms underlying the occurrence of these phenotypes and differentiate between function and effect of the knockout we compared the transcriptomes of cygb1 knockouts at different ages to age matched wild type WT zebrafish.", null, null, null, "Adult Brain F7", "F7 WT brain N R1 001", null, "strain:Tuebingen|isolate:WT Brain F7|age:12 mpf|dev stage:adult|sex:male|tissue:brain|genotype:wildtype|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNAseq Zebrafish 12mpf Brain WT", "F7 WT brain N", "F7 WT brain N", "Total RNA was extracted using the Crystal RNA Mini Kit following the instructions of the manufacturer. RNA samples were treated with DNaseI Qiagen  according to the manufacturers protocol. RNA concentration and quality for Illumina sequencing was measured by employing the TapeStation System 2200. 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Cygb might be involved in ROS detoxification  NO metabolism and lipid based signaling processes. Mammalian Cygb is ubiquitously expressed in all tissues. We used a CRISPR/Cas9 genome editing approach and generated a knockout of the penta coordinated cygb1 for in vivo analysis in zebrafish. Adult cygb1 knockouts develop phenotypic anomalies  including neoplasia of gonads  and weight loss. To identify the molecular mechanisms underlying the occurrence of these phenotypes and differentiate between function and effect of the knockout we compared the transcriptomes of cygb1 knockouts at different ages to age matched wild type WT zebrafish.", null, null, null, "Adult Brain F6", "F6 WT brain N R1 001", null, "strain:Tuebingen|isolate:WT Brain F6|age:12 mpf|dev stage:adult|sex:male|tissue:brain|genotype:wildtype|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNAseq Zebrafish 12mpf Brain WT", "F6 WT brain N", "F6 WT brain N", "Total RNA was extracted using the Crystal RNA Mini Kit following the instructions of the manufacturer. RNA samples were treated with DNaseI Qiagen  according to the manufacturers protocol. RNA concentration and quality for Illumina sequencing was measured by employing the TapeStation System 2200. 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