{"database": "metadata", "table": "run_metadata", "is_view": false, "human_description_en": "where devstage_curation_coarse = \"Juvenile\" and tissue_curation_coarse = \"Cardiovascular System\"", "rows": [[30738, "SRR28346485", "SRX23952998", "SRS20753493", "SRP495277", "PRJNA1088100", "Single cell transcriptional profiles of the atrioventricular canal of 1 mpf zebrafish treated with DMSO or IWR 1 endo", "GSE261604", "Transcriptome Analysis", "To identify the crosstalk between Wnt/\u00df catenin signaling activated cardiomyocytes \u00df cat ON CMs and coronary endothelial cells coECs  we performed single cell RNA sequencing on all the cells of the atrioventricular canal AVC at 1 month  which contains newly formed coronary vessels. To identify the extracellular ligands regulated by Wnt/\u00df catenin signaling  we also collected AVC from fish treated with DMSO or IWR1 endo  an inhibitor of Wnt/\u00df catenin signaling. Our analyses indicated that Fgf and/or Sema signaling are potential candidates for mediating the crosstalk between \u00df cat ON CMs and coECs. Overall design: We treated 1 mpf Tgcryaa:EGFP  myl7:GAL4db TCF?C;5xUAS:NTR mCherry fish with DMSO or 10 \u00b5M IWR1 endo for 48 hours. Hearts were resected from the fish treated with MTZ or DMSO  respectively. We isolated AVCs from hearts and dissociated them into single cells. Barcoded single cell cDNA libraries were prepared using the Chromium Next GEM single cell three primereagent kits v3.1 and sequenced using Illumina NextSeq 500.", "parent bioproject:PRJNA926354", "pubmed:39395410", null, "AVC  IWR1  scRNAseq", "GSM8147318", null, "source name:Heart|tissue:Heart|genotype:Tgfli1:EGFP;Tgcryaa:EGFP  myl7:GAL4db TCFdeltaC;5xUAS:NTR mCherry|treatment:IWR1|geo loc name:missing|collection date:missing", "AVC  IWR1  scRNAseq", "The 10x Genomics Cell Ranger pipeline v6.1.1 was used to perform sample demultiplexing  alignment to the reference genome Danio rerio GRCz11  barcode/UMI processing  and gene counting for each cell. Assembly: GRCz11 Supplementary files format and content: tsv files contain lists of genes and barcodes for each sample  mtx files count gene UMI couns for each sample", "Heart", "Fish were treated with DMSO or 10 \u03bcM MTZ dissolved in fish water for 48 h in the dark.", "Hearts were resected from 1 mpf Tgfli1:EGFP;Tgmyl7:GAL4db TCF\u0394C;5xUAS:NTR mCherry fish treated with DMSO or 10 \u03bcM MTZ for 48 h. The resected hearts were collected in 35 mm dishes containing HBSS without xxx and magnesium. The atrium and the ventricle were removed from AVCs. Only AVCs were transferred to a 12 well plate. The AVCs were digested with 3 mg/ml collagenase type IV Worthington  LS004188/HBSS for 30 min at 28.5 \u00baC. post pipetting  the cells were collected in low binding tubes  pelleted 3000 rpm  5 min at 4\u00baC  and washed in 0.5% FBS/DMEM phenol red free. Cells were resuspended in 0.5% FBS/DMEM and counted using a cell counter Thermofisher Scientific. The resulting cell suspension was loaded onto a 10X Chromium Controller 10X Genomics. The scRNA seq library was prepared using Chromium Next GEM single cell 3\u2019reagent kits v3.1 10X Genomics  1000128  according to the manufacturer\u2019s protocol. Following droplet generation and barcoding  cDNA was synthesized and amplified. The cDNA was processed to construct Illumine sequencing libraries. Sequencing was performed on a NextSeq 500 Illumina. Raw sequencing data were processed using 10X Genomics Cell Ranger software v6.1.1.", null, "tissue:Heart|genotype:Tgfli1:EGFP;Tgcryaa:EGFP  myl7:GAL4db TCFdeltaC;5xUAS:NTR mCherry|treatment:IWR1", "GSM8147318", "GSM8147318: AVC  IWR1  scRNAseq; Danio rerio; RNA Seq", "GSM8147318 r1", "GSM8147318", "1", "Hearts were resected from 1 mpf Tgfli1:EGFP;Tgmyl7:GAL4db TCF\u0394C;5xUAS:NTR mCherry fish treated with DMSO or 10 \u03bcM MTZ for 48 h. The resected hearts were collected in 35 mm dishes containing HBSS without xxx and magnesium. The atrium and the ventricle were removed from AVCs. Only AVCs were transferred to a 12 well plate. The AVCs were digested with 3 mg/ml collagenase type IV Worthington  LS004188/HBSS for 30 min at 28.5 \u00baC. post pipetting  the cells were collected in low binding tubes  pelleted 3000 rpm  5 min at 4\u00baC  and washed in 0.5% FBS/DMEM phenol red free. Cells were resuspended in 0.5% FBS/DMEM and counted using a cell counter Thermofisher Scientific. The resulting cell suspension was loaded onto a 10X Chromium Controller 10X Genomics. The scRNA seq library was prepared using Chromium Next GEM single cell three primereagent kits v3.1 10X Genomics  1000128  according to the manufacturer's protocol. Following droplet generation and barcoding  cDNA was synthesized and amplified. The cDNA was processed to construct Illumine sequencing libraries. Sequencing was performed on a NextSeq 500 Illumina. Raw sequencing data were processed using 10X Genomics Cell Ranger software v6.1.1.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 500", null, "SRP495277", null, "loader:fastq load.py", "AVC_IWR1_S1_L001_I1_001.fastq.gz AVC_IWR1_S1_L001_I2_001.fastq.gz AVC_IWR1_S1_L001_R1_001.fastq.gz AVC_IWR1_S1_L001_R2_001.fastq.gz", "fastq fastq fastq fastq", 8596842480.0, 62295960.0, "GSM8147318 r1", "0:10 1:10 2:28 3:90", "A:1570400090;C:1308581548;G:1326249813;T:1399966642;N:1438307", 10, 10, 28, 90, 1570400090, 1308581548, 1326249813, 1399966642, 1438307, "SRX23952998", "SRS20753493", "SRA1824323", "Department of Pharmacology, Yamagata University", "Department of Pharmacology, Yamagata University", null, null, null, null, null, null, null, null, null, null, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "Japan", "2024-03-14", "Juvenile", "Juvenile", "Heart", "Cardiovascular System"], [30739, "SRR28346486", "SRX23952998", "SRS20753493", "SRP495277", "PRJNA1088100", "Single cell transcriptional profiles of the atrioventricular canal of 1 mpf zebrafish treated with DMSO or IWR 1 endo", "GSE261604", "Transcriptome Analysis", "To identify the crosstalk between Wnt/\u00df catenin signaling activated cardiomyocytes \u00df cat ON CMs and coronary endothelial cells coECs  we performed single cell RNA sequencing on all the cells of the atrioventricular canal AVC at 1 month  which contains newly formed coronary vessels. To identify the extracellular ligands regulated by Wnt/\u00df catenin signaling  we also collected AVC from fish treated with DMSO or IWR1 endo  an inhibitor of Wnt/\u00df catenin signaling. Our analyses indicated that Fgf and/or Sema signaling are potential candidates for mediating the crosstalk between \u00df cat ON CMs and coECs. Overall design: We treated 1 mpf Tgcryaa:EGFP  myl7:GAL4db TCF?C;5xUAS:NTR mCherry fish with DMSO or 10 \u00b5M IWR1 endo for 48 hours. Hearts were resected from the fish treated with MTZ or DMSO  respectively. We isolated AVCs from hearts and dissociated them into single cells. Barcoded single cell cDNA libraries were prepared using the Chromium Next GEM single cell three primereagent kits v3.1 and sequenced using Illumina NextSeq 500.", "parent bioproject:PRJNA926354", "pubmed:39395410", null, "AVC  IWR1  scRNAseq", "GSM8147318", null, "source name:Heart|tissue:Heart|genotype:Tgfli1:EGFP;Tgcryaa:EGFP  myl7:GAL4db TCFdeltaC;5xUAS:NTR mCherry|treatment:IWR1|geo loc name:missing|collection date:missing", "AVC  IWR1  scRNAseq", "The 10x Genomics Cell Ranger pipeline v6.1.1 was used to perform sample demultiplexing  alignment to the reference genome Danio rerio GRCz11  barcode/UMI processing  and gene counting for each cell. Assembly: GRCz11 Supplementary files format and content: tsv files contain lists of genes and barcodes for each sample  mtx files count gene UMI couns for each sample", "Heart", "Fish were treated with DMSO or 10 \u03bcM MTZ dissolved in fish water for 48 h in the dark.", "Hearts were resected from 1 mpf Tgfli1:EGFP;Tgmyl7:GAL4db TCF\u0394C;5xUAS:NTR mCherry fish treated with DMSO or 10 \u03bcM MTZ for 48 h. The resected hearts were collected in 35 mm dishes containing HBSS without xxx and magnesium. The atrium and the ventricle were removed from AVCs. Only AVCs were transferred to a 12 well plate. The AVCs were digested with 3 mg/ml collagenase type IV Worthington  LS004188/HBSS for 30 min at 28.5 \u00baC. post pipetting  the cells were collected in low binding tubes  pelleted 3000 rpm  5 min at 4\u00baC  and washed in 0.5% FBS/DMEM phenol red free. Cells were resuspended in 0.5% FBS/DMEM and counted using a cell counter Thermofisher Scientific. The resulting cell suspension was loaded onto a 10X Chromium Controller 10X Genomics. The scRNA seq library was prepared using Chromium Next GEM single cell 3\u2019reagent kits v3.1 10X Genomics  1000128  according to the manufacturer\u2019s protocol. Following droplet generation and barcoding  cDNA was synthesized and amplified. The cDNA was processed to construct Illumine sequencing libraries. Sequencing was performed on a NextSeq 500 Illumina. Raw sequencing data were processed using 10X Genomics Cell Ranger software v6.1.1.", null, "tissue:Heart|genotype:Tgfli1:EGFP;Tgcryaa:EGFP  myl7:GAL4db TCFdeltaC;5xUAS:NTR mCherry|treatment:IWR1", "GSM8147318", "GSM8147318: AVC  IWR1  scRNAseq; Danio rerio; RNA Seq", "GSM8147318 r1", "GSM8147318", "1", "Hearts were resected from 1 mpf Tgfli1:EGFP;Tgmyl7:GAL4db TCF\u0394C;5xUAS:NTR mCherry fish treated with DMSO or 10 \u03bcM MTZ for 48 h. The resected hearts were collected in 35 mm dishes containing HBSS without xxx and magnesium. The atrium and the ventricle were removed from AVCs. Only AVCs were transferred to a 12 well plate. The AVCs were digested with 3 mg/ml collagenase type IV Worthington  LS004188/HBSS for 30 min at 28.5 \u00baC. post pipetting  the cells were collected in low binding tubes  pelleted 3000 rpm  5 min at 4\u00baC  and washed in 0.5% FBS/DMEM phenol red free. Cells were resuspended in 0.5% FBS/DMEM and counted using a cell counter Thermofisher Scientific. The resulting cell suspension was loaded onto a 10X Chromium Controller 10X Genomics. The scRNA seq library was prepared using Chromium Next GEM single cell three primereagent kits v3.1 10X Genomics  1000128  according to the manufacturer's protocol. Following droplet generation and barcoding  cDNA was synthesized and amplified. The cDNA was processed to construct Illumine sequencing libraries. Sequencing was performed on a NextSeq 500 Illumina. Raw sequencing data were processed using 10X Genomics Cell Ranger software v6.1.1.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 500", null, "SRP495277", null, "loader:fastq load.py", "AVC_IWR1_S1_L002_I1_001.fastq.gz AVC_IWR1_S1_L002_I2_001.fastq.gz AVC_IWR1_S1_L002_R1_001.fastq.gz AVC_IWR1_S1_L002_R2_001.fastq.gz", "fastq fastq fastq fastq", 8505185502.0, 61631779.0, "GSM8147318 r2", "0:10 1:10 2:28 3:90", "A:1553616390;C:1294687778;G:1312612882;T:1384504011;N:1439049", 10, 10, 28, 90, 1553616390, 1294687778, 1312612882, 1384504011, 1439049, "SRX23952998", "SRS20753493", "SRA1824323", "Department of Pharmacology, Yamagata University", "Department of Pharmacology, Yamagata University", null, null, null, null, null, null, null, null, null, null, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "Japan", "2024-03-14", "Juvenile", "Juvenile", "Heart", "Cardiovascular System"], [30740, "SRR28346487", "SRX23952998", "SRS20753493", "SRP495277", "PRJNA1088100", "Single cell transcriptional profiles of the atrioventricular canal of 1 mpf zebrafish treated with DMSO or IWR 1 endo", "GSE261604", "Transcriptome Analysis", "To identify the crosstalk between Wnt/\u00df catenin signaling activated cardiomyocytes \u00df cat ON CMs and coronary endothelial cells coECs  we performed single cell RNA sequencing on all the cells of the atrioventricular canal AVC at 1 month  which contains newly formed coronary vessels. To identify the extracellular ligands regulated by Wnt/\u00df catenin signaling  we also collected AVC from fish treated with DMSO or IWR1 endo  an inhibitor of Wnt/\u00df catenin signaling. Our analyses indicated that Fgf and/or Sema signaling are potential candidates for mediating the crosstalk between \u00df cat ON CMs and coECs. Overall design: We treated 1 mpf Tgcryaa:EGFP  myl7:GAL4db TCF?C;5xUAS:NTR mCherry fish with DMSO or 10 \u00b5M IWR1 endo for 48 hours. Hearts were resected from the fish treated with MTZ or DMSO  respectively. We isolated AVCs from hearts and dissociated them into single cells. Barcoded single cell cDNA libraries were prepared using the Chromium Next GEM single cell three primereagent kits v3.1 and sequenced using Illumina NextSeq 500.", "parent bioproject:PRJNA926354", "pubmed:39395410", null, "AVC  IWR1  scRNAseq", "GSM8147318", null, "source name:Heart|tissue:Heart|genotype:Tgfli1:EGFP;Tgcryaa:EGFP  myl7:GAL4db TCFdeltaC;5xUAS:NTR mCherry|treatment:IWR1|geo loc name:missing|collection date:missing", "AVC  IWR1  scRNAseq", "The 10x Genomics Cell Ranger pipeline v6.1.1 was used to perform sample demultiplexing  alignment to the reference genome Danio rerio GRCz11  barcode/UMI processing  and gene counting for each cell. Assembly: GRCz11 Supplementary files format and content: tsv files contain lists of genes and barcodes for each sample  mtx files count gene UMI couns for each sample", "Heart", "Fish were treated with DMSO or 10 \u03bcM MTZ dissolved in fish water for 48 h in the dark.", "Hearts were resected from 1 mpf Tgfli1:EGFP;Tgmyl7:GAL4db TCF\u0394C;5xUAS:NTR mCherry fish treated with DMSO or 10 \u03bcM MTZ for 48 h. The resected hearts were collected in 35 mm dishes containing HBSS without xxx and magnesium. The atrium and the ventricle were removed from AVCs. Only AVCs were transferred to a 12 well plate. The AVCs were digested with 3 mg/ml collagenase type IV Worthington  LS004188/HBSS for 30 min at 28.5 \u00baC. post pipetting  the cells were collected in low binding tubes  pelleted 3000 rpm  5 min at 4\u00baC  and washed in 0.5% FBS/DMEM phenol red free. Cells were resuspended in 0.5% FBS/DMEM and counted using a cell counter Thermofisher Scientific. The resulting cell suspension was loaded onto a 10X Chromium Controller 10X Genomics. The scRNA seq library was prepared using Chromium Next GEM single cell 3\u2019reagent kits v3.1 10X Genomics  1000128  according to the manufacturer\u2019s protocol. Following droplet generation and barcoding  cDNA was synthesized and amplified. The cDNA was processed to construct Illumine sequencing libraries. Sequencing was performed on a NextSeq 500 Illumina. Raw sequencing data were processed using 10X Genomics Cell Ranger software v6.1.1.", null, "tissue:Heart|genotype:Tgfli1:EGFP;Tgcryaa:EGFP  myl7:GAL4db TCFdeltaC;5xUAS:NTR mCherry|treatment:IWR1", "GSM8147318", "GSM8147318: AVC  IWR1  scRNAseq; Danio rerio; RNA Seq", "GSM8147318 r1", "GSM8147318", "1", "Hearts were resected from 1 mpf Tgfli1:EGFP;Tgmyl7:GAL4db TCF\u0394C;5xUAS:NTR mCherry fish treated with DMSO or 10 \u03bcM MTZ for 48 h. The resected hearts were collected in 35 mm dishes containing HBSS without xxx and magnesium. The atrium and the ventricle were removed from AVCs. Only AVCs were transferred to a 12 well plate. The AVCs were digested with 3 mg/ml collagenase type IV Worthington  LS004188/HBSS for 30 min at 28.5 \u00baC. post pipetting  the cells were collected in low binding tubes  pelleted 3000 rpm  5 min at 4\u00baC  and washed in 0.5% FBS/DMEM phenol red free. Cells were resuspended in 0.5% FBS/DMEM and counted using a cell counter Thermofisher Scientific. The resulting cell suspension was loaded onto a 10X Chromium Controller 10X Genomics. The scRNA seq library was prepared using Chromium Next GEM single cell three primereagent kits v3.1 10X Genomics  1000128  according to the manufacturer's protocol. Following droplet generation and barcoding  cDNA was synthesized and amplified. The cDNA was processed to construct Illumine sequencing libraries. Sequencing was performed on a NextSeq 500 Illumina. Raw sequencing data were processed using 10X Genomics Cell Ranger software v6.1.1.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 500", null, "SRP495277", null, "loader:fastq load.py", "AVC_IWR1_S1_L003_I1_001.fastq.gz AVC_IWR1_S1_L003_I2_001.fastq.gz AVC_IWR1_S1_L003_R1_001.fastq.gz AVC_IWR1_S1_L003_R2_001.fastq.gz", "fastq fastq fastq fastq", 8699205498.0, 63037721.0, "GSM8147318 r3", "0:10 1:10 2:28 3:90", "A:1588465122;C:1324147204;G:1343996634;T:1415623074;N:1162856", 10, 10, 28, 90, 1588465122, 1324147204, 1343996634, 1415623074, 1162856, "SRX23952998", "SRS20753493", "SRA1824323", "Department of Pharmacology, Yamagata University", "Department of Pharmacology, Yamagata University", null, null, null, null, null, null, null, null, null, null, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "Japan", "2024-03-14", "Juvenile", "Juvenile", "Heart", "Cardiovascular System"], [30741, "SRR28346488", "SRX23952998", "SRS20753493", "SRP495277", "PRJNA1088100", "Single cell transcriptional profiles of the atrioventricular canal of 1 mpf zebrafish treated with DMSO or IWR 1 endo", "GSE261604", "Transcriptome Analysis", "To identify the crosstalk between Wnt/\u00df catenin signaling activated cardiomyocytes \u00df cat ON CMs and coronary endothelial cells coECs  we performed single cell RNA sequencing on all the cells of the atrioventricular canal AVC at 1 month  which contains newly formed coronary vessels. To identify the extracellular ligands regulated by Wnt/\u00df catenin signaling  we also collected AVC from fish treated with DMSO or IWR1 endo  an inhibitor of Wnt/\u00df catenin signaling. Our analyses indicated that Fgf and/or Sema signaling are potential candidates for mediating the crosstalk between \u00df cat ON CMs and coECs. Overall design: We treated 1 mpf Tgcryaa:EGFP  myl7:GAL4db TCF?C;5xUAS:NTR mCherry fish with DMSO or 10 \u00b5M IWR1 endo for 48 hours. Hearts were resected from the fish treated with MTZ or DMSO  respectively. We isolated AVCs from hearts and dissociated them into single cells. Barcoded single cell cDNA libraries were prepared using the Chromium Next GEM single cell three primereagent kits v3.1 and sequenced using Illumina NextSeq 500.", "parent bioproject:PRJNA926354", "pubmed:39395410", null, "AVC  IWR1  scRNAseq", "GSM8147318", null, "source name:Heart|tissue:Heart|genotype:Tgfli1:EGFP;Tgcryaa:EGFP  myl7:GAL4db TCFdeltaC;5xUAS:NTR mCherry|treatment:IWR1|geo loc name:missing|collection date:missing", "AVC  IWR1  scRNAseq", "The 10x Genomics Cell Ranger pipeline v6.1.1 was used to perform sample demultiplexing  alignment to the reference genome Danio rerio GRCz11  barcode/UMI processing  and gene counting for each cell. Assembly: GRCz11 Supplementary files format and content: tsv files contain lists of genes and barcodes for each sample  mtx files count gene UMI couns for each sample", "Heart", "Fish were treated with DMSO or 10 \u03bcM MTZ dissolved in fish water for 48 h in the dark.", "Hearts were resected from 1 mpf Tgfli1:EGFP;Tgmyl7:GAL4db TCF\u0394C;5xUAS:NTR mCherry fish treated with DMSO or 10 \u03bcM MTZ for 48 h. The resected hearts were collected in 35 mm dishes containing HBSS without xxx and magnesium. The atrium and the ventricle were removed from AVCs. Only AVCs were transferred to a 12 well plate. The AVCs were digested with 3 mg/ml collagenase type IV Worthington  LS004188/HBSS for 30 min at 28.5 \u00baC. post pipetting  the cells were collected in low binding tubes  pelleted 3000 rpm  5 min at 4\u00baC  and washed in 0.5% FBS/DMEM phenol red free. Cells were resuspended in 0.5% FBS/DMEM and counted using a cell counter Thermofisher Scientific. The resulting cell suspension was loaded onto a 10X Chromium Controller 10X Genomics. The scRNA seq library was prepared using Chromium Next GEM single cell 3\u2019reagent kits v3.1 10X Genomics  1000128  according to the manufacturer\u2019s protocol. Following droplet generation and barcoding  cDNA was synthesized and amplified. The cDNA was processed to construct Illumine sequencing libraries. Sequencing was performed on a NextSeq 500 Illumina. Raw sequencing data were processed using 10X Genomics Cell Ranger software v6.1.1.", null, "tissue:Heart|genotype:Tgfli1:EGFP;Tgcryaa:EGFP  myl7:GAL4db TCFdeltaC;5xUAS:NTR mCherry|treatment:IWR1", "GSM8147318", "GSM8147318: AVC  IWR1  scRNAseq; Danio rerio; RNA Seq", "GSM8147318 r1", "GSM8147318", "1", "Hearts were resected from 1 mpf Tgfli1:EGFP;Tgmyl7:GAL4db TCF\u0394C;5xUAS:NTR mCherry fish treated with DMSO or 10 \u03bcM MTZ for 48 h. The resected hearts were collected in 35 mm dishes containing HBSS without xxx and magnesium. The atrium and the ventricle were removed from AVCs. Only AVCs were transferred to a 12 well plate. The AVCs were digested with 3 mg/ml collagenase type IV Worthington  LS004188/HBSS for 30 min at 28.5 \u00baC. post pipetting  the cells were collected in low binding tubes  pelleted 3000 rpm  5 min at 4\u00baC  and washed in 0.5% FBS/DMEM phenol red free. Cells were resuspended in 0.5% FBS/DMEM and counted using a cell counter Thermofisher Scientific. The resulting cell suspension was loaded onto a 10X Chromium Controller 10X Genomics. The scRNA seq library was prepared using Chromium Next GEM single cell three primereagent kits v3.1 10X Genomics  1000128  according to the manufacturer's protocol. Following droplet generation and barcoding  cDNA was synthesized and amplified. The cDNA was processed to construct Illumine sequencing libraries. Sequencing was performed on a NextSeq 500 Illumina. Raw sequencing data were processed using 10X Genomics Cell Ranger software v6.1.1.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 500", null, "SRP495277", null, "loader:fastq load.py", "AVC_IWR1_S1_L004_I1_001.fastq.gz AVC_IWR1_S1_L004_I2_001.fastq.gz AVC_IWR1_S1_L004_R1_001.fastq.gz AVC_IWR1_S1_L004_R2_001.fastq.gz", "fastq fastq fastq fastq", 8627728812.0, 62519774.0, "GSM8147318 r4", "0:10 1:10 2:28 3:90", "A:1576009383;C:1313466786;G:1332114241;T:1404392019;N:797231", 10, 10, 28, 90, 1576009383, 1313466786, 1332114241, 1404392019, 797231, "SRX23952998", "SRS20753493", "SRA1824323", "Department of Pharmacology, Yamagata University", "Department of Pharmacology, Yamagata University", null, null, null, null, null, null, null, null, null, null, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "Japan", "2024-03-14", "Juvenile", "Juvenile", "Heart", "Cardiovascular System"], [30742, "SRR28346489", "SRX23952997", "SRS20753492", "SRP495277", "PRJNA1088100", "Single cell transcriptional profiles of the atrioventricular canal of 1 mpf zebrafish treated with DMSO or IWR 1 endo", "GSE261604", "Transcriptome Analysis", "To identify the crosstalk between Wnt/\u00df catenin signaling activated cardiomyocytes \u00df cat ON CMs and coronary endothelial cells coECs  we performed single cell RNA sequencing on all the cells of the atrioventricular canal AVC at 1 month  which contains newly formed coronary vessels. To identify the extracellular ligands regulated by Wnt/\u00df catenin signaling  we also collected AVC from fish treated with DMSO or IWR1 endo  an inhibitor of Wnt/\u00df catenin signaling. Our analyses indicated that Fgf and/or Sema signaling are potential candidates for mediating the crosstalk between \u00df cat ON CMs and coECs. Overall design: We treated 1 mpf Tgcryaa:EGFP  myl7:GAL4db TCF?C;5xUAS:NTR mCherry fish with DMSO or 10 \u00b5M IWR1 endo for 48 hours. Hearts were resected from the fish treated with MTZ or DMSO  respectively. We isolated AVCs from hearts and dissociated them into single cells. Barcoded single cell cDNA libraries were prepared using the Chromium Next GEM single cell three primereagent kits v3.1 and sequenced using Illumina NextSeq 500.", "parent bioproject:PRJNA926354", "pubmed:39395410", null, "AVC  DMSO  scRNAseq", "GSM8147317", null, "source name:Heart|tissue:Heart|genotype:Tgfli1:EGFP;Tgcryaa:EGFP  myl7:GAL4db TCFdeltaC;5xUAS:NTR mCherry|treatment:DMSO|geo loc name:missing|collection date:missing", "AVC  DMSO  scRNAseq", "The 10x Genomics Cell Ranger pipeline v6.1.1 was used to perform sample demultiplexing  alignment to the reference genome Danio rerio GRCz11  barcode/UMI processing  and gene counting for each cell. Assembly: GRCz11 Supplementary files format and content: tsv files contain lists of genes and barcodes for each sample  mtx files count gene UMI couns for each sample", "Heart", "Fish were treated with DMSO or 10 \u03bcM MTZ dissolved in fish water for 48 h in the dark.", "Hearts were resected from 1 mpf Tgfli1:EGFP;Tgmyl7:GAL4db TCF\u0394C;5xUAS:NTR mCherry fish treated with DMSO or 10 \u03bcM MTZ for 48 h. The resected hearts were collected in 35 mm dishes containing HBSS without xxx and magnesium. The atrium and the ventricle were removed from AVCs. Only AVCs were transferred to a 12 well plate. The AVCs were digested with 3 mg/ml collagenase type IV Worthington  LS004188/HBSS for 30 min at 28.5 \u00baC. post pipetting  the cells were collected in low binding tubes  pelleted 3000 rpm  5 min at 4\u00baC  and washed in 0.5% FBS/DMEM phenol red free. Cells were resuspended in 0.5% FBS/DMEM and counted using a cell counter Thermofisher Scientific. The resulting cell suspension was loaded onto a 10X Chromium Controller 10X Genomics. The scRNA seq library was prepared using Chromium Next GEM single cell 3\u2019reagent kits v3.1 10X Genomics  1000128  according to the manufacturer\u2019s protocol. Following droplet generation and barcoding  cDNA was synthesized and amplified. The cDNA was processed to construct Illumine sequencing libraries. Sequencing was performed on a NextSeq 500 Illumina. Raw sequencing data were processed using 10X Genomics Cell Ranger software v6.1.1.", null, "tissue:Heart|genotype:Tgfli1:EGFP;Tgcryaa:EGFP  myl7:GAL4db TCFdeltaC;5xUAS:NTR mCherry|treatment:DMSO", "GSM8147317", "GSM8147317: AVC  DMSO  scRNAseq; Danio rerio; RNA Seq", "GSM8147317 r1", "GSM8147317", "1", "Hearts were resected from 1 mpf Tgfli1:EGFP;Tgmyl7:GAL4db TCF\u0394C;5xUAS:NTR mCherry fish treated with DMSO or 10 \u03bcM MTZ for 48 h. The resected hearts were collected in 35 mm dishes containing HBSS without xxx and magnesium. The atrium and the ventricle were removed from AVCs. Only AVCs were transferred to a 12 well plate. The AVCs were digested with 3 mg/ml collagenase type IV Worthington  LS004188/HBSS for 30 min at 28.5 \u00baC. post pipetting  the cells were collected in low binding tubes  pelleted 3000 rpm  5 min at 4\u00baC  and washed in 0.5% FBS/DMEM phenol red free. Cells were resuspended in 0.5% FBS/DMEM and counted using a cell counter Thermofisher Scientific. The resulting cell suspension was loaded onto a 10X Chromium Controller 10X Genomics. The scRNA seq library was prepared using Chromium Next GEM single cell three primereagent kits v3.1 10X Genomics  1000128  according to the manufacturer's protocol. Following droplet generation and barcoding  cDNA was synthesized and amplified. The cDNA was processed to construct Illumine sequencing libraries. Sequencing was performed on a NextSeq 500 Illumina. Raw sequencing data were processed using 10X Genomics Cell Ranger software v6.1.1.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 500", null, "SRP495277", null, "loader:fastq load.py", "AVC_Ctrl_S1_L001_I1_001.fastq.gz AVC_Ctrl_S1_L001_I2_001.fastq.gz AVC_Ctrl_S1_L001_R1_001.fastq.gz AVC_Ctrl_S1_L001_R2_001.fastq.gz", "fastq fastq fastq fastq", 7385103810.0, 53515245.0, "GSM8147317 r1", "0:10 1:10 2:28 3:90", "A:1329371520;C:1135299252;G:1143250549;T:1207226310;N:1224419", 10, 10, 28, 90, 1329371520, 1135299252, 1143250549, 1207226310, 1224419, "SRX23952997", "SRS20753492", "SRA1824323", "Department of Pharmacology, Yamagata University", "Department of Pharmacology, Yamagata University", null, null, null, null, null, null, null, null, null, null, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "Japan", "2024-03-14", "Juvenile", "Juvenile", "Heart", "Cardiovascular System"], [30743, "SRR28346490", "SRX23952997", "SRS20753492", "SRP495277", "PRJNA1088100", "Single cell transcriptional profiles of the atrioventricular canal of 1 mpf zebrafish treated with DMSO or IWR 1 endo", "GSE261604", "Transcriptome Analysis", "To identify the crosstalk between Wnt/\u00df catenin signaling activated cardiomyocytes \u00df cat ON CMs and coronary endothelial cells coECs  we performed single cell RNA sequencing on all the cells of the atrioventricular canal AVC at 1 month  which contains newly formed coronary vessels. To identify the extracellular ligands regulated by Wnt/\u00df catenin signaling  we also collected AVC from fish treated with DMSO or IWR1 endo  an inhibitor of Wnt/\u00df catenin signaling. Our analyses indicated that Fgf and/or Sema signaling are potential candidates for mediating the crosstalk between \u00df cat ON CMs and coECs. Overall design: We treated 1 mpf Tgcryaa:EGFP  myl7:GAL4db TCF?C;5xUAS:NTR mCherry fish with DMSO or 10 \u00b5M IWR1 endo for 48 hours. Hearts were resected from the fish treated with MTZ or DMSO  respectively. We isolated AVCs from hearts and dissociated them into single cells. Barcoded single cell cDNA libraries were prepared using the Chromium Next GEM single cell three primereagent kits v3.1 and sequenced using Illumina NextSeq 500.", "parent bioproject:PRJNA926354", "pubmed:39395410", null, "AVC  DMSO  scRNAseq", "GSM8147317", null, "source name:Heart|tissue:Heart|genotype:Tgfli1:EGFP;Tgcryaa:EGFP  myl7:GAL4db TCFdeltaC;5xUAS:NTR mCherry|treatment:DMSO|geo loc name:missing|collection date:missing", "AVC  DMSO  scRNAseq", "The 10x Genomics Cell Ranger pipeline v6.1.1 was used to perform sample demultiplexing  alignment to the reference genome Danio rerio GRCz11  barcode/UMI processing  and gene counting for each cell. Assembly: GRCz11 Supplementary files format and content: tsv files contain lists of genes and barcodes for each sample  mtx files count gene UMI couns for each sample", "Heart", "Fish were treated with DMSO or 10 \u03bcM MTZ dissolved in fish water for 48 h in the dark.", "Hearts were resected from 1 mpf Tgfli1:EGFP;Tgmyl7:GAL4db TCF\u0394C;5xUAS:NTR mCherry fish treated with DMSO or 10 \u03bcM MTZ for 48 h. The resected hearts were collected in 35 mm dishes containing HBSS without xxx and magnesium. The atrium and the ventricle were removed from AVCs. Only AVCs were transferred to a 12 well plate. The AVCs were digested with 3 mg/ml collagenase type IV Worthington  LS004188/HBSS for 30 min at 28.5 \u00baC. post pipetting  the cells were collected in low binding tubes  pelleted 3000 rpm  5 min at 4\u00baC  and washed in 0.5% FBS/DMEM phenol red free. Cells were resuspended in 0.5% FBS/DMEM and counted using a cell counter Thermofisher Scientific. The resulting cell suspension was loaded onto a 10X Chromium Controller 10X Genomics. The scRNA seq library was prepared using Chromium Next GEM single cell 3\u2019reagent kits v3.1 10X Genomics  1000128  according to the manufacturer\u2019s protocol. Following droplet generation and barcoding  cDNA was synthesized and amplified. The cDNA was processed to construct Illumine sequencing libraries. Sequencing was performed on a NextSeq 500 Illumina. Raw sequencing data were processed using 10X Genomics Cell Ranger software v6.1.1.", null, "tissue:Heart|genotype:Tgfli1:EGFP;Tgcryaa:EGFP  myl7:GAL4db TCFdeltaC;5xUAS:NTR mCherry|treatment:DMSO", "GSM8147317", "GSM8147317: AVC  DMSO  scRNAseq; Danio rerio; RNA Seq", "GSM8147317 r1", "GSM8147317", "1", "Hearts were resected from 1 mpf Tgfli1:EGFP;Tgmyl7:GAL4db TCF\u0394C;5xUAS:NTR mCherry fish treated with DMSO or 10 \u03bcM MTZ for 48 h. The resected hearts were collected in 35 mm dishes containing HBSS without xxx and magnesium. The atrium and the ventricle were removed from AVCs. Only AVCs were transferred to a 12 well plate. The AVCs were digested with 3 mg/ml collagenase type IV Worthington  LS004188/HBSS for 30 min at 28.5 \u00baC. post pipetting  the cells were collected in low binding tubes  pelleted 3000 rpm  5 min at 4\u00baC  and washed in 0.5% FBS/DMEM phenol red free. Cells were resuspended in 0.5% FBS/DMEM and counted using a cell counter Thermofisher Scientific. The resulting cell suspension was loaded onto a 10X Chromium Controller 10X Genomics. The scRNA seq library was prepared using Chromium Next GEM single cell three primereagent kits v3.1 10X Genomics  1000128  according to the manufacturer's protocol. Following droplet generation and barcoding  cDNA was synthesized and amplified. The cDNA was processed to construct Illumine sequencing libraries. Sequencing was performed on a NextSeq 500 Illumina. Raw sequencing data were processed using 10X Genomics Cell Ranger software v6.1.1.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 500", null, "SRP495277", null, "loader:fastq load.py", "AVC_Ctrl_S1_L002_I1_001.fastq.gz AVC_Ctrl_S1_L002_I2_001.fastq.gz AVC_Ctrl_S1_L002_R1_001.fastq.gz AVC_Ctrl_S1_L002_R2_001.fastq.gz", "fastq fastq fastq fastq", 7313299098.0, 52994921.0, "GSM8147317 r2", "0:10 1:10 2:28 3:90", "A:1316101332;C:1124200896;G:1133146934;T:1194866024;N:1227704", 10, 10, 28, 90, 1316101332, 1124200896, 1133146934, 1194866024, 1227704, "SRX23952997", "SRS20753492", "SRA1824323", "Department of Pharmacology, Yamagata University", "Department of Pharmacology, Yamagata University", null, null, null, null, null, null, null, null, null, null, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "Japan", "2024-03-14", "Juvenile", "Juvenile", "Heart", "Cardiovascular System"], [30744, "SRR28346491", "SRX23952997", "SRS20753492", "SRP495277", "PRJNA1088100", "Single cell transcriptional profiles of the atrioventricular canal of 1 mpf zebrafish treated with DMSO or IWR 1 endo", "GSE261604", "Transcriptome Analysis", "To identify the crosstalk between Wnt/\u00df catenin signaling activated cardiomyocytes \u00df cat ON CMs and coronary endothelial cells coECs  we performed single cell RNA sequencing on all the cells of the atrioventricular canal AVC at 1 month  which contains newly formed coronary vessels. To identify the extracellular ligands regulated by Wnt/\u00df catenin signaling  we also collected AVC from fish treated with DMSO or IWR1 endo  an inhibitor of Wnt/\u00df catenin signaling. Our analyses indicated that Fgf and/or Sema signaling are potential candidates for mediating the crosstalk between \u00df cat ON CMs and coECs. Overall design: We treated 1 mpf Tgcryaa:EGFP  myl7:GAL4db TCF?C;5xUAS:NTR mCherry fish with DMSO or 10 \u00b5M IWR1 endo for 48 hours. Hearts were resected from the fish treated with MTZ or DMSO  respectively. We isolated AVCs from hearts and dissociated them into single cells. Barcoded single cell cDNA libraries were prepared using the Chromium Next GEM single cell three primereagent kits v3.1 and sequenced using Illumina NextSeq 500.", "parent bioproject:PRJNA926354", "pubmed:39395410", null, "AVC  DMSO  scRNAseq", "GSM8147317", null, "source name:Heart|tissue:Heart|genotype:Tgfli1:EGFP;Tgcryaa:EGFP  myl7:GAL4db TCFdeltaC;5xUAS:NTR mCherry|treatment:DMSO|geo loc name:missing|collection date:missing", "AVC  DMSO  scRNAseq", "The 10x Genomics Cell Ranger pipeline v6.1.1 was used to perform sample demultiplexing  alignment to the reference genome Danio rerio GRCz11  barcode/UMI processing  and gene counting for each cell. Assembly: GRCz11 Supplementary files format and content: tsv files contain lists of genes and barcodes for each sample  mtx files count gene UMI couns for each sample", "Heart", "Fish were treated with DMSO or 10 \u03bcM MTZ dissolved in fish water for 48 h in the dark.", "Hearts were resected from 1 mpf Tgfli1:EGFP;Tgmyl7:GAL4db TCF\u0394C;5xUAS:NTR mCherry fish treated with DMSO or 10 \u03bcM MTZ for 48 h. The resected hearts were collected in 35 mm dishes containing HBSS without xxx and magnesium. The atrium and the ventricle were removed from AVCs. Only AVCs were transferred to a 12 well plate. The AVCs were digested with 3 mg/ml collagenase type IV Worthington  LS004188/HBSS for 30 min at 28.5 \u00baC. post pipetting  the cells were collected in low binding tubes  pelleted 3000 rpm  5 min at 4\u00baC  and washed in 0.5% FBS/DMEM phenol red free. Cells were resuspended in 0.5% FBS/DMEM and counted using a cell counter Thermofisher Scientific. The resulting cell suspension was loaded onto a 10X Chromium Controller 10X Genomics. The scRNA seq library was prepared using Chromium Next GEM single cell 3\u2019reagent kits v3.1 10X Genomics  1000128  according to the manufacturer\u2019s protocol. Following droplet generation and barcoding  cDNA was synthesized and amplified. The cDNA was processed to construct Illumine sequencing libraries. Sequencing was performed on a NextSeq 500 Illumina. Raw sequencing data were processed using 10X Genomics Cell Ranger software v6.1.1.", null, "tissue:Heart|genotype:Tgfli1:EGFP;Tgcryaa:EGFP  myl7:GAL4db TCFdeltaC;5xUAS:NTR mCherry|treatment:DMSO", "GSM8147317", "GSM8147317: AVC  DMSO  scRNAseq; Danio rerio; RNA Seq", "GSM8147317 r1", "GSM8147317", "1", "Hearts were resected from 1 mpf Tgfli1:EGFP;Tgmyl7:GAL4db TCF\u0394C;5xUAS:NTR mCherry fish treated with DMSO or 10 \u03bcM MTZ for 48 h. The resected hearts were collected in 35 mm dishes containing HBSS without xxx and magnesium. The atrium and the ventricle were removed from AVCs. Only AVCs were transferred to a 12 well plate. The AVCs were digested with 3 mg/ml collagenase type IV Worthington  LS004188/HBSS for 30 min at 28.5 \u00baC. post pipetting  the cells were collected in low binding tubes  pelleted 3000 rpm  5 min at 4\u00baC  and washed in 0.5% FBS/DMEM phenol red free. Cells were resuspended in 0.5% FBS/DMEM and counted using a cell counter Thermofisher Scientific. The resulting cell suspension was loaded onto a 10X Chromium Controller 10X Genomics. The scRNA seq library was prepared using Chromium Next GEM single cell three primereagent kits v3.1 10X Genomics  1000128  according to the manufacturer's protocol. Following droplet generation and barcoding  cDNA was synthesized and amplified. The cDNA was processed to construct Illumine sequencing libraries. Sequencing was performed on a NextSeq 500 Illumina. Raw sequencing data were processed using 10X Genomics Cell Ranger software v6.1.1.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 500", null, "SRP495277", null, "loader:fastq load.py", "AVC_Ctrl_S1_L003_I1_001.fastq.gz AVC_Ctrl_S1_L003_I2_001.fastq.gz AVC_Ctrl_S1_L003_R1_001.fastq.gz AVC_Ctrl_S1_L003_R2_001.fastq.gz", "fastq fastq fastq fastq", 7457857272.0, 54042444.0, "GSM8147317 r3", "0:10 1:10 2:28 3:90", "A:1341763332;C:1146520201;G:1156131488;T:1218395804;N:1009135", 10, 10, 28, 90, 1341763332, 1146520201, 1156131488, 1218395804, 1009135, "SRX23952997", "SRS20753492", "SRA1824323", "Department of Pharmacology, Yamagata University", "Department of Pharmacology, Yamagata University", null, null, null, null, null, null, null, null, null, null, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "Japan", "2024-03-14", "Juvenile", "Juvenile", "Heart", "Cardiovascular System"], [30745, "SRR28346492", "SRX23952997", "SRS20753492", "SRP495277", "PRJNA1088100", "Single cell transcriptional profiles of the atrioventricular canal of 1 mpf zebrafish treated with DMSO or IWR 1 endo", "GSE261604", "Transcriptome Analysis", "To identify the crosstalk between Wnt/\u00df catenin signaling activated cardiomyocytes \u00df cat ON CMs and coronary endothelial cells coECs  we performed single cell RNA sequencing on all the cells of the atrioventricular canal AVC at 1 month  which contains newly formed coronary vessels. To identify the extracellular ligands regulated by Wnt/\u00df catenin signaling  we also collected AVC from fish treated with DMSO or IWR1 endo  an inhibitor of Wnt/\u00df catenin signaling. Our analyses indicated that Fgf and/or Sema signaling are potential candidates for mediating the crosstalk between \u00df cat ON CMs and coECs. Overall design: We treated 1 mpf Tgcryaa:EGFP  myl7:GAL4db TCF?C;5xUAS:NTR mCherry fish with DMSO or 10 \u00b5M IWR1 endo for 48 hours. Hearts were resected from the fish treated with MTZ or DMSO  respectively. We isolated AVCs from hearts and dissociated them into single cells. Barcoded single cell cDNA libraries were prepared using the Chromium Next GEM single cell three primereagent kits v3.1 and sequenced using Illumina NextSeq 500.", "parent bioproject:PRJNA926354", "pubmed:39395410", null, "AVC  DMSO  scRNAseq", "GSM8147317", null, "source name:Heart|tissue:Heart|genotype:Tgfli1:EGFP;Tgcryaa:EGFP  myl7:GAL4db TCFdeltaC;5xUAS:NTR mCherry|treatment:DMSO|geo loc name:missing|collection date:missing", "AVC  DMSO  scRNAseq", "The 10x Genomics Cell Ranger pipeline v6.1.1 was used to perform sample demultiplexing  alignment to the reference genome Danio rerio GRCz11  barcode/UMI processing  and gene counting for each cell. Assembly: GRCz11 Supplementary files format and content: tsv files contain lists of genes and barcodes for each sample  mtx files count gene UMI couns for each sample", "Heart", "Fish were treated with DMSO or 10 \u03bcM MTZ dissolved in fish water for 48 h in the dark.", "Hearts were resected from 1 mpf Tgfli1:EGFP;Tgmyl7:GAL4db TCF\u0394C;5xUAS:NTR mCherry fish treated with DMSO or 10 \u03bcM MTZ for 48 h. The resected hearts were collected in 35 mm dishes containing HBSS without xxx and magnesium. The atrium and the ventricle were removed from AVCs. Only AVCs were transferred to a 12 well plate. The AVCs were digested with 3 mg/ml collagenase type IV Worthington  LS004188/HBSS for 30 min at 28.5 \u00baC. post pipetting  the cells were collected in low binding tubes  pelleted 3000 rpm  5 min at 4\u00baC  and washed in 0.5% FBS/DMEM phenol red free. Cells were resuspended in 0.5% FBS/DMEM and counted using a cell counter Thermofisher Scientific. The resulting cell suspension was loaded onto a 10X Chromium Controller 10X Genomics. The scRNA seq library was prepared using Chromium Next GEM single cell 3\u2019reagent kits v3.1 10X Genomics  1000128  according to the manufacturer\u2019s protocol. Following droplet generation and barcoding  cDNA was synthesized and amplified. The cDNA was processed to construct Illumine sequencing libraries. Sequencing was performed on a NextSeq 500 Illumina. Raw sequencing data were processed using 10X Genomics Cell Ranger software v6.1.1.", null, "tissue:Heart|genotype:Tgfli1:EGFP;Tgcryaa:EGFP  myl7:GAL4db TCFdeltaC;5xUAS:NTR mCherry|treatment:DMSO", "GSM8147317", "GSM8147317: AVC  DMSO  scRNAseq; Danio rerio; RNA Seq", "GSM8147317 r1", "GSM8147317", "1", "Hearts were resected from 1 mpf Tgfli1:EGFP;Tgmyl7:GAL4db TCF\u0394C;5xUAS:NTR mCherry fish treated with DMSO or 10 \u03bcM MTZ for 48 h. The resected hearts were collected in 35 mm dishes containing HBSS without xxx and magnesium. The atrium and the ventricle were removed from AVCs. Only AVCs were transferred to a 12 well plate. The AVCs were digested with 3 mg/ml collagenase type IV Worthington  LS004188/HBSS for 30 min at 28.5 \u00baC. post pipetting  the cells were collected in low binding tubes  pelleted 3000 rpm  5 min at 4\u00baC  and washed in 0.5% FBS/DMEM phenol red free. Cells were resuspended in 0.5% FBS/DMEM and counted using a cell counter Thermofisher Scientific. The resulting cell suspension was loaded onto a 10X Chromium Controller 10X Genomics. The scRNA seq library was prepared using Chromium Next GEM single cell three primereagent kits v3.1 10X Genomics  1000128  according to the manufacturer's protocol. Following droplet generation and barcoding  cDNA was synthesized and amplified. The cDNA was processed to construct Illumine sequencing libraries. Sequencing was performed on a NextSeq 500 Illumina. Raw sequencing data were processed using 10X Genomics Cell Ranger software v6.1.1.", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 500", null, "SRP495277", null, "loader:fastq load.py", "AVC_Ctrl_S1_L004_I1_001.fastq.gz AVC_Ctrl_S1_L004_I2_001.fastq.gz AVC_Ctrl_S1_L004_R1_001.fastq.gz AVC_Ctrl_S1_L004_R2_001.fastq.gz", "fastq fastq fastq fastq", 7430049030.0, 53840935.0, "GSM8147317 r4", "0:10 1:10 2:28 3:90", "A:1337094348;C:1142353903;G:1151539276;T:1214006711;N:689912", 10, 10, 28, 90, 1337094348, 1142353903, 1151539276, 1214006711, 689912, "SRX23952997", "SRS20753492", "SRA1824323", "Department of Pharmacology, Yamagata University", "Department of Pharmacology, Yamagata University", null, null, null, null, null, null, null, null, null, null, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "Japan", "2024-03-14", "Juvenile", "Juvenile", "Heart", "Cardiovascular System"], [68893, "SRR18218072", "SRX14364503", "SRS12177798", "SRP362416", "PRJNA812715", "Mutant IL7R collaborates with MYC to induce  T cell Acute Lymphoblastic Leukemia", "PRJNA812715", "Other", "T cell acute lymphoblastic leukemia T ALL is an aggressive pediatric cancer. Amongst the wide array of driver mutations  10% of T ALL patients display gain of function mutations in the IL 7 receptor alpha chain IL 7Ralpha  encoded by IL7R  which occur in different molecular subtypes of this disease. However  it is still unclear whether IL 7R mutational activation is sufficient to transform T cell precursors. Also  which genes cooperate with IL7R to drive leukemogenesis remain poorly defined. Here  we demonstrate that mutant IL7R alone is capable of inducing T ALL with long latency in stable transgenic zebrafish and transformation is associated with MYC transcriptional activation. Additionally  we find that mutant IL7R collaborates with Myc to induce early onset T ALL in transgenic zebrafish. T ALLs co expressing mutant IL7R and Myc show activation of STAT5 and AKT pathways  harbor reduced numbers of apoptotic cells and remake tumors in transplanted zebrafish faster than T ALLs expressing Myc alone. Moreover  limiting dilution cell transplantation experiments reveal that activated IL 7R signaling increases the overall frequency of leukemia propagating cells. Our work highlights a synergy between mutant IL7R and Myc in inducing T ALL and demonstrates that mutant IL7R enriches for leukemia propagating potential.", null, null, null, null, "Tu AB mMyc 7", null, "strain:Tu/AB|age:41 days|sex:not collected|tissue:lymphoid|birth date:04 11 2016|death date:15 12 2016|genotype:rag2:mCherry rag2:mMyc|biological replicate:Tu AB mMyc biological replicate 3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA seq of Danio rerio leukemia overexpressing mouse Myc Tu/AB", "Tu AB mMyc 7", "Tu AB mMyc 7", "Tu/AB zebrafish overexpressing mouse Myc transiently were sacrificed when moribund and sorted leukemias were harvested for further analysis. The RNA was extracted from sorted cells using the RNeasy Mini Kit according to the manufacturers instructions Qiagen. mRNA was enriched using magnetic beads with Oligo dT  fragmented and converted to cDNA  size selected and PCR amplified generating paired end 100 bp sequences using a Illumina NovaSeq 6000.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP362416", null, null, "FCHT5GHDSXX_L1_HKRDZEBmfpEAAXRAAPEI-P87H3_1.fq.gz FCHT5GHDSXX_L1_HKRDZEBmfpEAAXRAAPEI-P87H3_2.fq.gz", "fastq fastq", 18002105400.0, 60007018.0, "FCHT5GHDSXX L1 HKRDZEBmfpEAAXRAAPEI P87H3 1.fq.gz", "0:150 1:150", "A:4483057959;C:4549819065;G:4562924506;T:4406277865;N:26005", 150, 150, null, null, 4483057959, 4549819065, 4562924506, 4406277865, 26005, "SRX14364503", "SRS12177798", "SRA1380603", "Instituto de Medicina Molecular Joao Lobo Antunes|JBarata lab", "Instituto de Medicina Molecular Joao Lobo Antunes", 2, 0.9259, 0.92681, 0.09642, 0.09697, 0.82479, 0.82546, 0.50634, 0.5148, 150, 150, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "Portugal", "2022-03-04", "Juvenile", "Juvenile", "Lymphatic System", "Cardiovascular System"], [68894, "SRR18218073", "SRX14364502", "SRS12177797", "SRP362416", "PRJNA812715", "Mutant IL7R collaborates with MYC to induce  T cell Acute Lymphoblastic Leukemia", "PRJNA812715", "Other", "T cell acute lymphoblastic leukemia T ALL is an aggressive pediatric cancer. Amongst the wide array of driver mutations  10% of T ALL patients display gain of function mutations in the IL 7 receptor alpha chain IL 7Ralpha  encoded by IL7R  which occur in different molecular subtypes of this disease. However  it is still unclear whether IL 7R mutational activation is sufficient to transform T cell precursors. Also  which genes cooperate with IL7R to drive leukemogenesis remain poorly defined. Here  we demonstrate that mutant IL7R alone is capable of inducing T ALL with long latency in stable transgenic zebrafish and transformation is associated with MYC transcriptional activation. Additionally  we find that mutant IL7R collaborates with Myc to induce early onset T ALL in transgenic zebrafish. T ALLs co expressing mutant IL7R and Myc show activation of STAT5 and AKT pathways  harbor reduced numbers of apoptotic cells and remake tumors in transplanted zebrafish faster than T ALLs expressing Myc alone. Moreover  limiting dilution cell transplantation experiments reveal that activated IL 7R signaling increases the overall frequency of leukemia propagating cells. Our work highlights a synergy between mutant IL7R and Myc in inducing T ALL and demonstrates that mutant IL7R enriches for leukemia propagating potential.", null, null, null, null, "Tu AB mMyc 6", null, "strain:Tu/AB|age:42 days|sex:not collected|tissue:lymphoid|birth date:03 11 2016|death date:15 12 2016|genotype:rag2:mCherry rag2:mMyc|biological replicate:Tu AB mMyc biological replicate 2|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA seq of Danio rerio leukemia overexpressing mouse Myc Tu/AB", "Tu AB mMyc 6", "Tu AB mMyc 6", "Tu/AB zebrafish overexpressing mouse Myc transiently were sacrificed when moribund and sorted leukemias were harvested for further analysis. The RNA was extracted from sorted cells using the RNeasy Mini Kit according to the manufacturers instructions Qiagen. mRNA was enriched using magnetic beads with Oligo dT  fragmented and converted to cDNA  size selected and PCR amplified generating paired end 100 bp sequences using a Illumina NovaSeq 6000.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP362416", null, null, "FCHT5GHDSXX_L1_HKRDZEBmfpEAAWRABPEI-P75G3_1.fq.gz FCHT5GHDSXX_L1_HKRDZEBmfpEAAWRABPEI-P75G3_2.fq.gz", "fastq fastq", 18085463400.0, 60284878.0, "FCHT5GHDSXX L1 HKRDZEBmfpEAAWRABPEI P75G3 1.fq.gz", "0:150 1:150", "A:4716166539;C:4371919988;G:4399782048;T:4597568953;N:25872", 150, 150, null, null, 4716166539, 4371919988, 4399782048, 4597568953, 25872, "SRX14364502", "SRS12177797", "SRA1380603", "Instituto de Medicina Molecular Joao Lobo Antunes|JBarata lab", "Instituto de Medicina Molecular Joao Lobo Antunes", 2, 0.90432, 0.90596, 0.06444, 0.06367, 0.80048, 0.80095, 0.51671, 0.51624, 150, 150, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "Portugal", "2022-03-04", "Juvenile", "Juvenile", "Lymphatic System", "Cardiovascular System"], [68895, "SRR18218074", "SRX14364501", "SRS12177796", "SRP362416", "PRJNA812715", "Mutant IL7R collaborates with MYC to induce  T cell Acute Lymphoblastic Leukemia", "PRJNA812715", "Other", "T cell acute lymphoblastic leukemia T ALL is an aggressive pediatric cancer. Amongst the wide array of driver mutations  10% of T ALL patients display gain of function mutations in the IL 7 receptor alpha chain IL 7Ralpha  encoded by IL7R  which occur in different molecular subtypes of this disease. However  it is still unclear whether IL 7R mutational activation is sufficient to transform T cell precursors. Also  which genes cooperate with IL7R to drive leukemogenesis remain poorly defined. Here  we demonstrate that mutant IL7R alone is capable of inducing T ALL with long latency in stable transgenic zebrafish and transformation is associated with MYC transcriptional activation. Additionally  we find that mutant IL7R collaborates with Myc to induce early onset T ALL in transgenic zebrafish. T ALLs co expressing mutant IL7R and Myc show activation of STAT5 and AKT pathways  harbor reduced numbers of apoptotic cells and remake tumors in transplanted zebrafish faster than T ALLs expressing Myc alone. Moreover  limiting dilution cell transplantation experiments reveal that activated IL 7R signaling increases the overall frequency of leukemia propagating cells. Our work highlights a synergy between mutant IL7R and Myc in inducing T ALL and demonstrates that mutant IL7R enriches for leukemia propagating potential.", null, null, null, null, "Tu AB mMyc 5", null, "strain:Tu/AB|age:42 days|sex:not collected|tissue:lymphoid|birth date:03 11 2016|death date:15 12 2016|genotype:rag2:mCherry rag2:mMyc|biological replicate:Tu AB mMyc biological replicate 1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA seq of Danio rerio leukemia overexpressing mouse Myc Tu/AB", "Tu AB mMyc 5", "Tu AB mMyc 5", "Tu/AB zebrafish overexpressing mouse Myc transiently were sacrificed when moribund and sorted leukemias were harvested for further analysis. The RNA was extracted from sorted cells using the RNeasy Mini Kit according to the manufacturers instructions Qiagen. mRNA was enriched using magnetic beads with Oligo dT  fragmented and converted to cDNA  size selected and PCR amplified generating paired end 100 bp sequences using a Illumina NovaSeq 6000.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP362416", null, null, "FCHT5GHDSXX_L1_HKRDZEBmfpEAAVRAAPEI-P63F3_1.fq.gz FCHT5GHDSXX_L1_HKRDZEBmfpEAAVRAAPEI-P63F3_2.fq.gz", "fastq fastq", 18063312600.0, 60211042.0, "FCHT5GHDSXX L1 HKRDZEBmfpEAAVRAAPEI P63F3 1.fq.gz", "0:150 1:150", "A:4440821008;C:4618318980;G:4635597240;T:4368548938;N:26434", 150, 150, null, null, 4440821008, 4618318980, 4635597240, 4368548938, 26434, "SRX14364501", "SRS12177796", "SRA1380603", "Instituto de Medicina Molecular Joao Lobo Antunes|JBarata lab", "Instituto de Medicina Molecular Joao Lobo Antunes", 2, 0.91708, 0.91735, 0.0319, 0.0316, 0.80793, 0.80744, 0.50004, 0.5071, 150, 150, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "Portugal", "2022-03-04", "Juvenile", "Juvenile", "Lymphatic System", "Cardiovascular System"], [68898, "SRR18218077", "SRX14364498", "SRS12177793", "SRP362416", "PRJNA812715", "Mutant IL7R collaborates with MYC to induce  T cell Acute Lymphoblastic Leukemia", "PRJNA812715", "Other", "T cell acute lymphoblastic leukemia T ALL is an aggressive pediatric cancer. Amongst the wide array of driver mutations  10% of T ALL patients display gain of function mutations in the IL 7 receptor alpha chain IL 7Ralpha  encoded by IL7R  which occur in different molecular subtypes of this disease. However  it is still unclear whether IL 7R mutational activation is sufficient to transform T cell precursors. Also  which genes cooperate with IL7R to drive leukemogenesis remain poorly defined. Here  we demonstrate that mutant IL7R alone is capable of inducing T ALL with long latency in stable transgenic zebrafish and transformation is associated with MYC transcriptional activation. Additionally  we find that mutant IL7R collaborates with Myc to induce early onset T ALL in transgenic zebrafish. T ALLs co expressing mutant IL7R and Myc show activation of STAT5 and AKT pathways  harbor reduced numbers of apoptotic cells and remake tumors in transplanted zebrafish faster than T ALLs expressing Myc alone. Moreover  limiting dilution cell transplantation experiments reveal that activated IL 7R signaling increases the overall frequency of leukemia propagating cells. Our work highlights a synergy between mutant IL7R and Myc in inducing T ALL and demonstrates that mutant IL7R enriches for leukemia propagating potential.", null, null, null, null, "Tu AB mMyc+P1mut 6", null, "strain:Tu/AB|age:35 days|sex:not collected|tissue:lymphoid|birth date:30 08 2016|death date:04 10 2016|genotype:rag2:mCherry rag2:mMyc rag2:hIL7R mut1|biological replicate:Tu AB mMyc+P1mut biological replicate 3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA seq of Danio rerio leukemia overexpressing mouse Myc and human mutant IL7R Tu/AB", "Tu AB mMyc+P1mut 6", "Tu AB mMyc+P1mut 6", "Tu/AB zebrafish overexpressing mouse Myc and human mutant IL7R transiently were sacrificed when moribund and sorted leukemias were harvested for further analysis. The RNA was extracted from sorted cells using the RNeasy Mini Kit according to the manufacturers instructions Qiagen. mRNA was enriched using magnetic beads with Oligo dT  fragmented and converted to cDNA  size selected and PCR amplified generating paired end 100 bp sequences using a Illumina NovaSeq 6000.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP362416", null, null, "FCHT5GHDSXX_L1_HKRDZEBmfpEABARAAPEI-P28C4_1.fq.gz FCHT5GHDSXX_L1_HKRDZEBmfpEABARAAPEI-P28C4_2.fq.gz", "fastq fastq", 18034321800.0, 60114406.0, "FCHT5GHDSXX L1 HKRDZEBmfpEABARAAPEI P28C4 1.fq.gz", "0:150 1:150", "A:4518122158;C:4519676234;G:4543758117;T:4452738564;N:26727", 150, 150, null, null, 4518122158, 4519676234, 4543758117, 4452738564, 26727, "SRX14364498", "SRS12177793", "SRA1380603", "Instituto de Medicina Molecular Joao Lobo Antunes|JBarata lab", "Instituto de Medicina Molecular Joao Lobo Antunes", 2, 0.94431, 0.94437, 0.10494, 0.10462, 0.83177, 0.83037, 0.51143, 0.51642, 150, 150, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "Portugal", "2022-03-04", "Juvenile", "Juvenile", "Lymphatic System", "Cardiovascular System"], [68899, "SRR18218078", "SRX14364497", "SRS12177792", "SRP362416", "PRJNA812715", "Mutant IL7R collaborates with MYC to induce  T cell Acute Lymphoblastic Leukemia", "PRJNA812715", "Other", "T cell acute lymphoblastic leukemia T ALL is an aggressive pediatric cancer. Amongst the wide array of driver mutations  10% of T ALL patients display gain of function mutations in the IL 7 receptor alpha chain IL 7Ralpha  encoded by IL7R  which occur in different molecular subtypes of this disease. However  it is still unclear whether IL 7R mutational activation is sufficient to transform T cell precursors. Also  which genes cooperate with IL7R to drive leukemogenesis remain poorly defined. Here  we demonstrate that mutant IL7R alone is capable of inducing T ALL with long latency in stable transgenic zebrafish and transformation is associated with MYC transcriptional activation. Additionally  we find that mutant IL7R collaborates with Myc to induce early onset T ALL in transgenic zebrafish. T ALLs co expressing mutant IL7R and Myc show activation of STAT5 and AKT pathways  harbor reduced numbers of apoptotic cells and remake tumors in transplanted zebrafish faster than T ALLs expressing Myc alone. Moreover  limiting dilution cell transplantation experiments reveal that activated IL 7R signaling increases the overall frequency of leukemia propagating cells. Our work highlights a synergy between mutant IL7R and Myc in inducing T ALL and demonstrates that mutant IL7R enriches for leukemia propagating potential.", null, null, null, null, "Tu AB mMyc+P1mut 5", null, "strain:Tu/AB|age:35 days|sex:not collected|tissue:lymphoid|birth date:30 08 2016|death date:04 10 2016|genotype:rag2:mCherry rag2:mMyc rag2:hIL7R mut1|biological replicate:Tu AB mMyc+P1mut biological replicate 2|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA seq of Danio rerio leukemia overexpressing mouse Myc and human mutant IL7R Tu/AB", "Tu AB mMyc+P1mut 5", "Tu AB mMyc+P1mut 5", "Tu/AB zebrafish overexpressing mouse Myc and human mutant IL7R transiently were sacrificed when moribund and sorted leukemias were harvested for further analysis. The RNA was extracted from sorted cells using the RNeasy Mini Kit according to the manufacturers instructions Qiagen. mRNA was enriched using magnetic beads with Oligo dT  fragmented and converted to cDNA  size selected and PCR amplified generating paired end 100 bp sequences using a Illumina NovaSeq 6000.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP362416", null, null, "FCHT5GHDSXX_L1_HKRDZEBmfpEAAZRAAPEI-P16B4_1.fq.gz FCHT5GHDSXX_L1_HKRDZEBmfpEAAZRAAPEI-P16B4_2.fq.gz", "fastq fastq", 17750634300.0, 59168781.0, "FCHT5GHDSXX L1 HKRDZEBmfpEAAZRAAPEI P16B4 1.fq.gz", "0:150 1:150", "A:4503506819;C:4398697723;G:4432972998;T:4415430837;N:25923", 150, 150, null, null, 4503506819, 4398697723, 4432972998, 4415430837, 25923, "SRX14364497", "SRS12177792", "SRA1380603", "Instituto de Medicina Molecular Joao Lobo Antunes|JBarata lab", "Instituto de Medicina Molecular Joao Lobo Antunes", 2, 0.94306, 0.94321, 0.07511, 0.0748, 0.79837, 0.79819, 0.51326, 0.50902, 150, 150, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "Portugal", "2022-03-04", "Juvenile", "Juvenile", "Lymphatic System", "Cardiovascular System"], [68900, "SRR18218079", "SRX14364496", "SRS12177791", "SRP362416", "PRJNA812715", "Mutant IL7R collaborates with MYC to induce  T cell Acute Lymphoblastic Leukemia", "PRJNA812715", "Other", "T cell acute lymphoblastic leukemia T ALL is an aggressive pediatric cancer. Amongst the wide array of driver mutations  10% of T ALL patients display gain of function mutations in the IL 7 receptor alpha chain IL 7Ralpha  encoded by IL7R  which occur in different molecular subtypes of this disease. However  it is still unclear whether IL 7R mutational activation is sufficient to transform T cell precursors. Also  which genes cooperate with IL7R to drive leukemogenesis remain poorly defined. Here  we demonstrate that mutant IL7R alone is capable of inducing T ALL with long latency in stable transgenic zebrafish and transformation is associated with MYC transcriptional activation. Additionally  we find that mutant IL7R collaborates with Myc to induce early onset T ALL in transgenic zebrafish. T ALLs co expressing mutant IL7R and Myc show activation of STAT5 and AKT pathways  harbor reduced numbers of apoptotic cells and remake tumors in transplanted zebrafish faster than T ALLs expressing Myc alone. Moreover  limiting dilution cell transplantation experiments reveal that activated IL 7R signaling increases the overall frequency of leukemia propagating cells. Our work highlights a synergy between mutant IL7R and Myc in inducing T ALL and demonstrates that mutant IL7R enriches for leukemia propagating potential.", null, null, null, null, "Tu AB mMyc+P1mut 4", null, "strain:Tu/AB|age:35 days|sex:not collected|tissue:lymphoid|birth date:30 08 2016|death date:04 10 2016|genotype:rag2:mCherry rag2:mMyc rag2:hIL7R mut1|biological replicate:Tu AB mMyc+P1mut biological replicate 1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA seq of Danio rerio leukemia overexpressing mouse Myc and human mutant IL7R Tu/AB", "Tu AB mMyc+P1mut 4", "Tu AB mMyc+P1mut 4", "Tu/AB zebrafish overexpressing mouse Myc and human mutant IL7R transiently were sacrificed when moribund and sorted leukemias were harvested for further analysis. The RNA was extracted from sorted cells using the RNeasy Mini Kit according to the manufacturers instructions Qiagen. mRNA was enriched using magnetic beads with Oligo dT  fragmented and converted to cDNA  size selected and PCR amplified generating paired end 100 bp sequences using a Illumina NovaSeq 6000.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PolyA", "PAIRED", "ILLUMINA", "Illumina NovaSeq 6000", null, "SRP362416", null, null, "FCHT5GHDSXX_L1_HKRDZEBmfpEAAYRAAPEI-P4A4_1.fq.gz FCHT5GHDSXX_L1_HKRDZEBmfpEAAYRAAPEI-P4A4_2.fq.gz", "fastq fastq", 18063110700.0, 60210369.0, "FCHT5GHDSXX L1 HKRDZEBmfpEAAYRAAPEI P4A4 1.fq.gz", "0:150 1:150", "A:4583950532;C:4496933352;G:4489104841;T:4493095718;N:26257", 150, 150, null, null, 4583950532, 4496933352, 4489104841, 4493095718, 26257, "SRX14364496", "SRS12177791", "SRA1380603", "Instituto de Medicina Molecular Joao Lobo Antunes|JBarata lab", "Instituto de Medicina Molecular Joao Lobo Antunes", 2, 0.95231, 0.9534, 0.12525, 0.12554, 0.84404, 0.84323, 0.52132, 0.53078, 150, 150, "B", "B", "biological fallback assumption", "illumina", "novaseq_era", "unknown", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "Portugal", "2022-03-04", "Juvenile", "Juvenile", "Lymphatic System", "Cardiovascular System"], [71807, "SRR22058034", "SRX18039091", "SRS15545647", "SRP404721", "PRJNA894591", "hapln1a+ cells guide coronary growth during heart morphogenesis and regeneration [Dev  hapln1a+ cells]", "GSE216646", "Other", "As cardiac regeneration requires new coronary vessels  exploring the underlying mechanisms behind revascularization will facilitate the development of regenerative therapies for heart failure. Although multiple tissues and chemokines likely orchestrate coronary formation  the interaction between coronary growth and guidance cues remains unclear. Here  by applying single cell RNA sequencing scRNA seq analysis  we examined gene expression in zebrafish epicardial cells during coronary vascularization and identified hapln1a expressing epicardial cells enriched with vascular regulating genes. Fluorescence reporter assays indicated hapln1a+ cells not only envelop coronary vessels  but also form cellular shear structures in ahead of coronary tips. Live imaging analyses demonstrated coronary growth along the pre formed shears  with depletion of hapln1a+ cells blocking this growth. Further  we found hapln1a+ cells also pre lead coronary tips in the regenerating area and hapln1a+ cell loss inhibits coronary revascularization. To characterize the molecular nature of hapln1a+ cells during coronary growth  we profiled hapln1a+ cells in juvenile and regenerating hearts and detected expression of the cell adhesion and migration regulator serpine1 in hapln1a+ cells adjacent to coronary tips. Pharmacological inhibition of serpine1 function blocked coronary vascularization and revascularization. Altogether  our studies reveal that hapln1a+ cells are required for coronary production during heart morphogenesis and regeneration  by establishing a microenvironment to facilitate guided coronary growth. Overall design: hapln1a+ cells of zebrafish heart were isolated by Fluorescence activated cell sorting FACS according to the presence or absence of eGFP signal and analyzed using scRNAseq.", "parent bioproject:PRJNA894685", "pubmed:37311876", null, "hapln1a+  development  scRNAseq", "GSM6685234", null, "source name:heart|strain:EK|tissue:heart|cell type:epicardial cells|age:7 weeks|geo loc name:missing|collection date:missing", "hapln1a+  development  scRNAseq", "All processing was performed in 10X Genomics CellRanger v3.1.0 using default parameters Assembly: Danio rerio GRCz11 Supplementary files format and content: Cell Ranger outputs a .mtx file with the feature barcode matrix. This matrix contains the counts of molecules per cell UMI/cell as determined post filtering and counting by Cell Ranger. The matrix is in market exchange format while the row gene or feature and columns cell barcodes are provided as TSV files. File formats are documented in the Cell Ranger software manual", "heart", null, "Hearts were dissected from juvenile zebrafish  and ventricles were digested with Liberase TM 0.26 U/ml . Dissociated cells were spun down and live EGFP+ cells were sorted by flow cytometry. Library was performed according to the manufacter\u2019s instructions single cell 3\u2019 v3.1 protocol  10x Genomics ", null, "strain:EK|tissue:heart|cell type:epicardial cells|cell type sorted:hapln1a+|age:7 weeks", "GSM6685234", "GSM6685234: hapln1a+  development  scRNAseq; Danio rerio; RNA Seq", "GSM6685234 r1", "GSM6685234", "1", "Hearts were dissected from juvenile zebrafish  and ventricles were digested with Liberase TM 0.26 U/ml . Dissociated cells were spun down and live EGFP+ cells were sorted by flow cytometry. Library was performed according to the manufacter's instructions single cell three prime v3.1 protocol  10x Genomics ", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 550", null, "SRP404721", null, "loader:fastq load.py", "1_S1_L001_I1_001.fastq.gz 1_S1_L001_R1_001.fastq.gz 1_S1_L001_R2_001.fastq.gz", "fastq fastq fastq", 1606360897.0, 12648511.0, "GSM6685234 r1", "0:8 1:28 2:91", "A:339791226;C:243552181;G:266423271;T:301107277;N:140546", 8, 28, 91, null, 339791226, 243552181, 266423271, 301107277, 140546, "SRX18039091", "SRS15545647", "SRA1528482", "Emory University", "Emory University", 1, 0.92176, null, 0.1499, null, 0.81781, null, 0.50927, null, 91, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "United States", "2022-10-26", "Juvenile", "Juvenile", "Heart", "Cardiovascular System"], [71808, "SRR22058035", "SRX18039091", "SRS15545647", "SRP404721", "PRJNA894591", "hapln1a+ cells guide coronary growth during heart morphogenesis and regeneration [Dev  hapln1a+ cells]", "GSE216646", "Other", "As cardiac regeneration requires new coronary vessels  exploring the underlying mechanisms behind revascularization will facilitate the development of regenerative therapies for heart failure. Although multiple tissues and chemokines likely orchestrate coronary formation  the interaction between coronary growth and guidance cues remains unclear. Here  by applying single cell RNA sequencing scRNA seq analysis  we examined gene expression in zebrafish epicardial cells during coronary vascularization and identified hapln1a expressing epicardial cells enriched with vascular regulating genes. Fluorescence reporter assays indicated hapln1a+ cells not only envelop coronary vessels  but also form cellular shear structures in ahead of coronary tips. Live imaging analyses demonstrated coronary growth along the pre formed shears  with depletion of hapln1a+ cells blocking this growth. Further  we found hapln1a+ cells also pre lead coronary tips in the regenerating area and hapln1a+ cell loss inhibits coronary revascularization. To characterize the molecular nature of hapln1a+ cells during coronary growth  we profiled hapln1a+ cells in juvenile and regenerating hearts and detected expression of the cell adhesion and migration regulator serpine1 in hapln1a+ cells adjacent to coronary tips. Pharmacological inhibition of serpine1 function blocked coronary vascularization and revascularization. Altogether  our studies reveal that hapln1a+ cells are required for coronary production during heart morphogenesis and regeneration  by establishing a microenvironment to facilitate guided coronary growth. Overall design: hapln1a+ cells of zebrafish heart were isolated by Fluorescence activated cell sorting FACS according to the presence or absence of eGFP signal and analyzed using scRNAseq.", "parent bioproject:PRJNA894685", "pubmed:37311876", null, "hapln1a+  development  scRNAseq", "GSM6685234", null, "source name:heart|strain:EK|tissue:heart|cell type:epicardial cells|age:7 weeks|geo loc name:missing|collection date:missing", "hapln1a+  development  scRNAseq", "All processing was performed in 10X Genomics CellRanger v3.1.0 using default parameters Assembly: Danio rerio GRCz11 Supplementary files format and content: Cell Ranger outputs a .mtx file with the feature barcode matrix. This matrix contains the counts of molecules per cell UMI/cell as determined post filtering and counting by Cell Ranger. The matrix is in market exchange format while the row gene or feature and columns cell barcodes are provided as TSV files. File formats are documented in the Cell Ranger software manual", "heart", null, "Hearts were dissected from juvenile zebrafish  and ventricles were digested with Liberase TM 0.26 U/ml . Dissociated cells were spun down and live EGFP+ cells were sorted by flow cytometry. Library was performed according to the manufacter\u2019s instructions single cell 3\u2019 v3.1 protocol  10x Genomics ", null, "strain:EK|tissue:heart|cell type:epicardial cells|cell type sorted:hapln1a+|age:7 weeks", "GSM6685234", "GSM6685234: hapln1a+  development  scRNAseq; Danio rerio; RNA Seq", "GSM6685234 r1", "GSM6685234", "1", "Hearts were dissected from juvenile zebrafish  and ventricles were digested with Liberase TM 0.26 U/ml . Dissociated cells were spun down and live EGFP+ cells were sorted by flow cytometry. Library was performed according to the manufacter's instructions single cell three prime v3.1 protocol  10x Genomics ", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 550", null, "SRP404721", null, "loader:fastq load.py", "1_S2_L001_I1_001.fastq.gz 1_S2_L001_R1_001.fastq.gz 1_S2_L001_R2_001.fastq.gz", "fastq fastq fastq", 1332426088.0, 10491544.0, "GSM6685234 r2", "0:8 1:28 2:91", "A:281795062;C:202126091;G:221122322;T:249574256;N:112773", 8, 28, 91, null, 281795062, 202126091, 221122322, 249574256, 112773, "SRX18039091", "SRS15545647", "SRA1528482", "Emory University", "Emory University", 1, 0.92141, null, 0.14909, null, 0.81714, null, 0.50543, null, 91, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "United States", "2022-10-26", "Juvenile", "Juvenile", "Heart", "Cardiovascular System"], [71809, "SRR22058036", "SRX18039091", "SRS15545647", "SRP404721", "PRJNA894591", "hapln1a+ cells guide coronary growth during heart morphogenesis and regeneration [Dev  hapln1a+ cells]", "GSE216646", "Other", "As cardiac regeneration requires new coronary vessels  exploring the underlying mechanisms behind revascularization will facilitate the development of regenerative therapies for heart failure. Although multiple tissues and chemokines likely orchestrate coronary formation  the interaction between coronary growth and guidance cues remains unclear. Here  by applying single cell RNA sequencing scRNA seq analysis  we examined gene expression in zebrafish epicardial cells during coronary vascularization and identified hapln1a expressing epicardial cells enriched with vascular regulating genes. Fluorescence reporter assays indicated hapln1a+ cells not only envelop coronary vessels  but also form cellular shear structures in ahead of coronary tips. Live imaging analyses demonstrated coronary growth along the pre formed shears  with depletion of hapln1a+ cells blocking this growth. Further  we found hapln1a+ cells also pre lead coronary tips in the regenerating area and hapln1a+ cell loss inhibits coronary revascularization. To characterize the molecular nature of hapln1a+ cells during coronary growth  we profiled hapln1a+ cells in juvenile and regenerating hearts and detected expression of the cell adhesion and migration regulator serpine1 in hapln1a+ cells adjacent to coronary tips. Pharmacological inhibition of serpine1 function blocked coronary vascularization and revascularization. Altogether  our studies reveal that hapln1a+ cells are required for coronary production during heart morphogenesis and regeneration  by establishing a microenvironment to facilitate guided coronary growth. Overall design: hapln1a+ cells of zebrafish heart were isolated by Fluorescence activated cell sorting FACS according to the presence or absence of eGFP signal and analyzed using scRNAseq.", "parent bioproject:PRJNA894685", "pubmed:37311876", null, "hapln1a+  development  scRNAseq", "GSM6685234", null, "source name:heart|strain:EK|tissue:heart|cell type:epicardial cells|age:7 weeks|geo loc name:missing|collection date:missing", "hapln1a+  development  scRNAseq", "All processing was performed in 10X Genomics CellRanger v3.1.0 using default parameters Assembly: Danio rerio GRCz11 Supplementary files format and content: Cell Ranger outputs a .mtx file with the feature barcode matrix. This matrix contains the counts of molecules per cell UMI/cell as determined post filtering and counting by Cell Ranger. The matrix is in market exchange format while the row gene or feature and columns cell barcodes are provided as TSV files. File formats are documented in the Cell Ranger software manual", "heart", null, "Hearts were dissected from juvenile zebrafish  and ventricles were digested with Liberase TM 0.26 U/ml . Dissociated cells were spun down and live EGFP+ cells were sorted by flow cytometry. Library was performed according to the manufacter\u2019s instructions single cell 3\u2019 v3.1 protocol  10x Genomics ", null, "strain:EK|tissue:heart|cell type:epicardial cells|cell type sorted:hapln1a+|age:7 weeks", "GSM6685234", "GSM6685234: hapln1a+  development  scRNAseq; Danio rerio; RNA Seq", "GSM6685234 r1", "GSM6685234", "1", "Hearts were dissected from juvenile zebrafish  and ventricles were digested with Liberase TM 0.26 U/ml . Dissociated cells were spun down and live EGFP+ cells were sorted by flow cytometry. Library was performed according to the manufacter's instructions single cell three prime v3.1 protocol  10x Genomics ", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 550", null, "SRP404721", null, "loader:fastq load.py", "1_S3_L001_I1_001.fastq.gz 1_S3_L001_R1_001.fastq.gz 1_S3_L001_R2_001.fastq.gz", "fastq fastq fastq", 1187072175.0, 9347025.0, "GSM6685234 r3", "0:8 1:28 2:91", "A:251072030;C:180002392;G:196984629;T:222418470;N:101754", 8, 28, 91, null, 251072030, 180002392, 196984629, 222418470, 101754, "SRX18039091", "SRS15545647", "SRA1528482", "Emory University", "Emory University", 1, 0.92231, null, 0.15014, null, 0.8174, null, 0.50735, null, 91, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "United States", "2022-10-26", "Juvenile", "Juvenile", "Heart", "Cardiovascular System"], [71810, "SRR22058037", "SRX18039091", "SRS15545647", "SRP404721", "PRJNA894591", "hapln1a+ cells guide coronary growth during heart morphogenesis and regeneration [Dev  hapln1a+ cells]", "GSE216646", "Other", "As cardiac regeneration requires new coronary vessels  exploring the underlying mechanisms behind revascularization will facilitate the development of regenerative therapies for heart failure. Although multiple tissues and chemokines likely orchestrate coronary formation  the interaction between coronary growth and guidance cues remains unclear. Here  by applying single cell RNA sequencing scRNA seq analysis  we examined gene expression in zebrafish epicardial cells during coronary vascularization and identified hapln1a expressing epicardial cells enriched with vascular regulating genes. Fluorescence reporter assays indicated hapln1a+ cells not only envelop coronary vessels  but also form cellular shear structures in ahead of coronary tips. Live imaging analyses demonstrated coronary growth along the pre formed shears  with depletion of hapln1a+ cells blocking this growth. Further  we found hapln1a+ cells also pre lead coronary tips in the regenerating area and hapln1a+ cell loss inhibits coronary revascularization. To characterize the molecular nature of hapln1a+ cells during coronary growth  we profiled hapln1a+ cells in juvenile and regenerating hearts and detected expression of the cell adhesion and migration regulator serpine1 in hapln1a+ cells adjacent to coronary tips. Pharmacological inhibition of serpine1 function blocked coronary vascularization and revascularization. Altogether  our studies reveal that hapln1a+ cells are required for coronary production during heart morphogenesis and regeneration  by establishing a microenvironment to facilitate guided coronary growth. Overall design: hapln1a+ cells of zebrafish heart were isolated by Fluorescence activated cell sorting FACS according to the presence or absence of eGFP signal and analyzed using scRNAseq.", "parent bioproject:PRJNA894685", "pubmed:37311876", null, "hapln1a+  development  scRNAseq", "GSM6685234", null, "source name:heart|strain:EK|tissue:heart|cell type:epicardial cells|age:7 weeks|geo loc name:missing|collection date:missing", "hapln1a+  development  scRNAseq", "All processing was performed in 10X Genomics CellRanger v3.1.0 using default parameters Assembly: Danio rerio GRCz11 Supplementary files format and content: Cell Ranger outputs a .mtx file with the feature barcode matrix. This matrix contains the counts of molecules per cell UMI/cell as determined post filtering and counting by Cell Ranger. The matrix is in market exchange format while the row gene or feature and columns cell barcodes are provided as TSV files. File formats are documented in the Cell Ranger software manual", "heart", null, "Hearts were dissected from juvenile zebrafish  and ventricles were digested with Liberase TM 0.26 U/ml . Dissociated cells were spun down and live EGFP+ cells were sorted by flow cytometry. Library was performed according to the manufacter\u2019s instructions single cell 3\u2019 v3.1 protocol  10x Genomics ", null, "strain:EK|tissue:heart|cell type:epicardial cells|cell type sorted:hapln1a+|age:7 weeks", "GSM6685234", "GSM6685234: hapln1a+  development  scRNAseq; Danio rerio; RNA Seq", "GSM6685234 r1", "GSM6685234", "1", "Hearts were dissected from juvenile zebrafish  and ventricles were digested with Liberase TM 0.26 U/ml . Dissociated cells were spun down and live EGFP+ cells were sorted by flow cytometry. Library was performed according to the manufacter's instructions single cell three prime v3.1 protocol  10x Genomics ", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 550", null, "SRP404721", null, "loader:fastq load.py", "1_S4_L001_I1_001.fastq.gz 1_S4_L001_R1_001.fastq.gz 1_S4_L001_R2_001.fastq.gz", "fastq fastq fastq", 1362894785.0, 10731455.0, "GSM6685234 r4", "0:8 1:28 2:91", "A:288451488;C:206540819;G:226008729;T:255442217;N:119152", 8, 28, 91, null, 288451488, 206540819, 226008729, 255442217, 119152, "SRX18039091", "SRS15545647", "SRA1528482", "Emory University", "Emory University", 1, 0.92215, null, 0.15108, null, 0.81706, null, 0.51589, null, 91, null, "B", null, "usable mapping rate", "illumina", "nextseq", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "United States", "2022-10-26", "Juvenile", "Juvenile", "Heart", "Cardiovascular System"], [71811, "SRR22058440", "SRX18039491", "SRS15546048", "SRP404726", "PRJNA894687", "hapln1a+ cells guide coronary growth during heart morphogenesis and regeneration [Dev  tcf21+ cells]", "GSE216648", "Other", "As cardiac regeneration requires new coronary vessels  exploring the underlying mechanisms behind revascularization will facilitate the development of regenerative therapies for heart failure. Although multiple tissues and chemokines likely orchestrate coronary formation  the interaction between coronary growth and guidance cues remains unclear. Here  by applying single cell RNA sequencing scRNA seq analysis  we examined gene expression in zebrafish epicardial cells during coronary vascularization and identified hapln1a expressing epicardial cells enriched with vascular regulating genes. Fluorescence reporter assays indicated hapln1a+ cells not only envelop coronary vessels  but also form cellular shear structures in ahead of coronary tips. Live imaging analyses demonstrated coronary growth along the pre formed shears  with depletion of hapln1a+ cells blocking this growth. Further  we found hapln1a+ cells also pre lead coronary tips in the regenerating area and hapln1a+ cell loss inhibits coronary revascularization. To characterize the molecular nature of hapln1a+ cells during coronary growth  we profiled hapln1a+ cells in juvenile and regenerating hearts and detected expression of the cell adhesion and migration regulator serpine1 in hapln1a+ cells adjacent to coronary tips. Pharmacological inhibition of serpine1 function blocked coronary vascularization and revascularization. Altogether  our studies reveal that hapln1a+ cells are required for coronary production during heart morphogenesis and regeneration  by establishing a microenvironment to facilitate guided coronary growth. Overall design: Epicardial cells of zebrafish heart were isolated by Fluorescence activated cell sorting FACS according to the presence or absence of eGFP signal and analyzed using scRNAseq.", "parent bioproject:PRJNA894685", "pubmed:37311876", null, "tcf21+  development  scRNAseq", "GSM6685266", null, "source name:heart|strain:EK|tissue:heart|cell type:epicardial cells|age:7 weeks|geo loc name:missing|collection date:missing", "tcf21+  development  scRNAseq", "All processing was performed in 10X Genomics CellRanger v3.1.0 using default parameters Assembly: Danio rerio GRCz11 Supplementary files format and content: Cell Ranger outputs a .mtx file with the feature barcode matrix. This matrix contains the counts of molecules per cell UMI/cell as determined post filtering and counting by Cell Ranger. The matrix is in market exchange format while the row gene or feature and columns cell barcodes are provided as TSV files. File formats are documented in the Cell Ranger software manual", "heart", null, "Hearts were dissected from juvenile zebrafish  and ventricles were digested with Liberase TM 0.26 U/ml . Dissociated cells were spun down and live EGFP+ cells were sorted by flow cytometry. Library was performed according to the manufacter\u2019s instructions single cell 3\u2019 v3.1 protocol  10x Genomics ", null, "strain:EK|tissue:heart|cell type:epicardial cells|cell type sorted:tcf21+|age:7 weeks", "GSM6685266", "GSM6685266: tcf21+  development  scRNAseq; Danio rerio; RNA Seq", "GSM6685266 r1", "GSM6685266", "1", "Hearts were dissected from juvenile zebrafish  and ventricles were digested with Liberase TM 0.26 U/ml . Dissociated cells were spun down and live EGFP+ cells were sorted by flow cytometry. Library was performed according to the manufacter's instructions single cell three prime v3.1 protocol  10x Genomics ", null, "RNA-Seq", "TRANSCRIPTOMIC SINGLE CELL", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 550", null, "SRP404726", null, null, "TCF_21_S1_L001_R1_001.fastq.gz TCF_21_S1_L001_R2_001.fastq.gz", "fastq fastq", 19362384447.0, 162709113.0, "GSM6685266 r1", "0:28 1:91", "A:5391284818;C:4382019183;G:4643235756;T:4943804153;N:2040537", 28, 91, null, null, 5391284818, 4382019183, 4643235756, 4943804153, 2040537, "SRX18039491", "SRS15546048", "SRA1528514", "Emory University", "Emory University", 2, 0.00432, 0.90419, 0.00171, 0.10489, 0.9947, 0.84737, 0.31827, 0.50898, 28, 91, "T", "B", "sc-like readlen", "illumina", "nextseq", "unknown", "cdna_unspecified", "unknown", "sc", "single_cell_droplet", "10x", null, "United States", "2022-10-26", "Juvenile", "Juvenile", "Heart", "Cardiovascular System"], [76452, "SRR24950188", "SRX20708357", "SRS18001302", "SRP444408", "PRJNA984676", "RNA Seq analyses of myocardium specific hey2  overexpressing zebrafish hearts and Adv Hey2 infected NRVMs", "PRJNA984676", "Other", "To elucidate the molecular underpinning of cardiac dilation and myocardial mitochondrial dysfunction caused by hey2 induction  we conducted RNA seq analyses in hey2 overexpression zebrafish hearts and control littermate hearts at 1 week or 1 month post 4 HT treatments. To further investigate the impact of Hey2 in mammalian cardiomyocytes and mitochondria  we performed comparative analysis of transcriptome profiles on NRVMs infected with adenovirus containing Hey2 Adv Hey2 or vectors Adv Ctrl.", null, null, null, "RNA Seq for myocardium specific hey2 overexpressing heart at 1 week post 4 HT treatments  rep1", "OE1w1", null, "strain:not applicable|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:1 month|dev stage:not applicable|collection date:not applicable|geo loc name:not applicable|sex:male and female|tissue:Heart|ID:OE1w replicate 1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq for myocardium specific hey2 overexpressing heart at 1 week post 4 HT treatments  rep1", "OE1w replicate 1", "OE1w replicate 1", "RNA Seq for myocardium specific hey2 overexpressing heart at 1 week post 4 HT treatments  replicate 1", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PCR", "PAIRED", "BGISEQ", "BGISEQ-500", null, "SRP444408", null, null, "OE1w1_1.fq.gz OE1w1_2.fq.gz", "fastq fastq", 6792141900.0, 22640473.0, "OE1w1 1.fq.gz", "0:150 1:150", "A:1821318465;C:1573309073;G:1556399365;T:1841095017;N:19980", 150, 150, null, null, 1821318465, 1573309073, 1556399365, 1841095017, 19980, "SRX20708357", "SRS18001302", "SRA1657339", "East China Normal University|School of Life Sciences", "East China Normal University", 2, 0.92576, 0.91367, 0.07095, 0.07022, 0.74884, 0.75012, 0.52564, 0.52623, 150, 150, "B", "B", "biological fallback assumption", "bgi", "bgi", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2023-06-16", "Juvenile", "Juvenile", "Heart", "Cardiovascular System"], [76453, "SRR24950189", "SRX20708356", "SRS18001301", "SRP444408", "PRJNA984676", "RNA Seq analyses of myocardium specific hey2  overexpressing zebrafish hearts and Adv Hey2 infected NRVMs", "PRJNA984676", "Other", "To elucidate the molecular underpinning of cardiac dilation and myocardial mitochondrial dysfunction caused by hey2 induction  we conducted RNA seq analyses in hey2 overexpression zebrafish hearts and control littermate hearts at 1 week or 1 month post 4 HT treatments. To further investigate the impact of Hey2 in mammalian cardiomyocytes and mitochondria  we performed comparative analysis of transcriptome profiles on NRVMs infected with adenovirus containing Hey2 Adv Hey2 or vectors Adv Ctrl.", null, null, null, "RNA Seq for control littermate hearts at 1 week post 4 HT treatments  rep3", "CTL1w3", null, "strain:not applicable|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:1 month|dev stage:not applicable|collection date:not applicable|geo loc name:not applicable|sex:male and female|tissue:Heart|ID:CTL1w replicate 3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq for control littermate hearts at 1 week post 4 HT treatments  rep3", "CTL1w replicate 3", "CTL1w replicate 3", "RNA Seq for control littermate hearts at 1 week post 4 HT treatments  replicate 3", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PCR", "PAIRED", "BGISEQ", "BGISEQ-500", null, "SRP444408", null, null, "CTL1w3_2.fq.gz CTL1w3_1.fq.gz", "fastq fastq", 6615956700.0, 22053189.0, "CTL1w3 1.fq.gz", "0:150 1:150", "A:1790825663;C:1516487260;G:1497904437;T:1810720169;N:19171", 150, 150, null, null, 1790825663, 1516487260, 1497904437, 1810720169, 19171, "SRX20708356", "SRS18001301", "SRA1657339", "East China Normal University|School of Life Sciences", "East China Normal University", 2, 0.93183, 0.91562, 0.07118, 0.07012, 0.7655, 0.7682, 0.53912, 0.52899, 150, 150, "B", "B", "biological fallback assumption", "bgi", "bgi", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2023-06-16", "Juvenile", "Juvenile", "Heart", "Cardiovascular System"], [76454, "SRR24950190", "SRX20708355", "SRS18001300", "SRP444408", "PRJNA984676", "RNA Seq analyses of myocardium specific hey2  overexpressing zebrafish hearts and Adv Hey2 infected NRVMs", "PRJNA984676", "Other", "To elucidate the molecular underpinning of cardiac dilation and myocardial mitochondrial dysfunction caused by hey2 induction  we conducted RNA seq analyses in hey2 overexpression zebrafish hearts and control littermate hearts at 1 week or 1 month post 4 HT treatments. To further investigate the impact of Hey2 in mammalian cardiomyocytes and mitochondria  we performed comparative analysis of transcriptome profiles on NRVMs infected with adenovirus containing Hey2 Adv Hey2 or vectors Adv Ctrl.", null, null, null, "RNA Seq for control littermate hearts at 1 week post 4 HT treatments  rep2", "CTL1w2", null, "strain:not applicable|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:1 month|dev stage:not applicable|collection date:not applicable|geo loc name:not applicable|sex:male and female|tissue:Heart|ID:CTL1w replicate 2|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq for control littermate hearts at 1 week post 4 HT treatments  rep2", "CTL1w replicate 2", "CTL1w replicate 2", "RNA Seq for control littermate hearts at 1 week post 4 HT treatments  replicate 2", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PCR", "PAIRED", "BGISEQ", "BGISEQ-500", null, "SRP444408", null, null, "CTL1w2_1.fq.gz CTL1w2_2.fq.gz", "fastq fastq", 6582133800.0, 21940446.0, "CTL1w2 1.fq.gz", "0:150 1:150", "A:1766322301;C:1524014072;G:1506429130;T:1785350030;N:18267", 150, 150, null, null, 1766322301, 1524014072, 1506429130, 1785350030, 18267, "SRX20708355", "SRS18001300", "SRA1657339", "East China Normal University|School of Life Sciences", "East China Normal University", 2, 0.93867, 0.92389, 0.06545, 0.06458, 0.76357, 0.76583, 0.53821, 0.54255, 150, 150, "B", "B", "biological fallback assumption", "bgi", "bgi", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2023-06-16", "Juvenile", "Juvenile", "Heart", "Cardiovascular System"], [76455, "SRR24950191", "SRX20708354", "SRS18001299", "SRP444408", "PRJNA984676", "RNA Seq analyses of myocardium specific hey2  overexpressing zebrafish hearts and Adv Hey2 infected NRVMs", "PRJNA984676", "Other", "To elucidate the molecular underpinning of cardiac dilation and myocardial mitochondrial dysfunction caused by hey2 induction  we conducted RNA seq analyses in hey2 overexpression zebrafish hearts and control littermate hearts at 1 week or 1 month post 4 HT treatments. To further investigate the impact of Hey2 in mammalian cardiomyocytes and mitochondria  we performed comparative analysis of transcriptome profiles on NRVMs infected with adenovirus containing Hey2 Adv Hey2 or vectors Adv Ctrl.", null, null, null, "RNA Seq for control littermate hearts at 1 week post 4 HT treatments  rep1", "CTL1w1", null, "strain:not applicable|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:1 month|dev stage:not applicable|collection date:not applicable|geo loc name:not applicable|sex:male and female|tissue:Heart|ID:CTL1w replicate 1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq for control littermate hearts at 1 week post 4 HT treatments  rep1", "CTL1w replicate 1", "CTL1w replicate 1", "RNA Seq for control littermate hearts at 1 week post 4 HT treatments  replicate 1", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PCR", "PAIRED", "BGISEQ", "BGISEQ-500", null, "SRP444408", null, null, "CTL1w1_2.fq.gz CTL1w1_1.fq.gz", "fastq fastq", 6781172100.0, 22603907.0, "CTL1w1 1.fq.gz", "0:150 1:150", "A:1804085074;C:1581571692;G:1567430366;T:1828066356;N:18612", 150, 150, null, null, 1804085074, 1581571692, 1567430366, 1828066356, 18612, "SRX20708354", "SRS18001299", "SRA1657339", "East China Normal University|School of Life Sciences", "East China Normal University", 2, 0.93867, 0.91921, 0.06237, 0.06146, 0.75858, 0.76246, 0.53972, 0.53069, 150, 150, "B", "B", "biological fallback assumption", "bgi", "bgi", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2023-06-16", "Juvenile", "Juvenile", "Heart", "Cardiovascular System"], [76460, "SRR24950198", "SRX20708347", "SRS18001292", "SRP444408", "PRJNA984676", "RNA Seq analyses of myocardium specific hey2  overexpressing zebrafish hearts and Adv Hey2 infected NRVMs", "PRJNA984676", "Other", "To elucidate the molecular underpinning of cardiac dilation and myocardial mitochondrial dysfunction caused by hey2 induction  we conducted RNA seq analyses in hey2 overexpression zebrafish hearts and control littermate hearts at 1 week or 1 month post 4 HT treatments. To further investigate the impact of Hey2 in mammalian cardiomyocytes and mitochondria  we performed comparative analysis of transcriptome profiles on NRVMs infected with adenovirus containing Hey2 Adv Hey2 or vectors Adv Ctrl.", null, null, null, "RNA Seq for myocardium specific hey2 overexpressing heart at 1 week post 4 HT treatments  rep3", "OE1w3", null, "strain:not applicable|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:1 month|dev stage:not applicable|collection date:not applicable|geo loc name:not applicable|sex:male and female|tissue:Heart|ID:OE1w replicate 3|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq for myocardium specific hey2 overexpressing heart at 1 week post 4 HT treatments  rep3", "OE1w replicate 3", "OE1w replicate 3", "RNA Seq for myocardium specific hey2 overexpressing heart at 1 week post 4 HT treatments  replicate 3", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PCR", "PAIRED", "BGISEQ", "BGISEQ-500", null, "SRP444408", null, null, "OE1w3_2.fq.gz OE1w3_1.fq.gz", "fastq fastq", 6593130600.0, 21977102.0, "OE1w3 1.fq.gz", "0:150 1:150", "A:1770061078;C:1529682678;G:1510949508;T:1782338558;N:98778", 150, 150, null, null, 1770061078, 1529682678, 1510949508, 1782338558, 98778, "SRX20708347", "SRS18001292", "SRA1657339", "East China Normal University|School of Life Sciences", "East China Normal University", 2, 0.93503, 0.922, 0.06588, 0.06574, 0.7503, 0.75201, 0.52969, 0.53743, 150, 150, "B", "B", "biological fallback assumption", "bgi", "bgi", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2023-06-16", "Juvenile", "Juvenile", "Heart", "Cardiovascular System"], [76461, "SRR24950199", "SRX20708346", "SRS18001291", "SRP444408", "PRJNA984676", "RNA Seq analyses of myocardium specific hey2  overexpressing zebrafish hearts and Adv Hey2 infected NRVMs", "PRJNA984676", "Other", "To elucidate the molecular underpinning of cardiac dilation and myocardial mitochondrial dysfunction caused by hey2 induction  we conducted RNA seq analyses in hey2 overexpression zebrafish hearts and control littermate hearts at 1 week or 1 month post 4 HT treatments. To further investigate the impact of Hey2 in mammalian cardiomyocytes and mitochondria  we performed comparative analysis of transcriptome profiles on NRVMs infected with adenovirus containing Hey2 Adv Hey2 or vectors Adv Ctrl.", null, null, null, "RNA Seq for myocardium specific hey2 overexpressing heart at 1 week post 4 HT treatments  rep2", "OE1w2", null, "strain:not applicable|isolate:not applicable|breed:not applicable|cultivar:not applicable|ecotype:not applicable|age:1 month|dev stage:not applicable|collection date:not applicable|geo loc name:not applicable|sex:male and female|tissue:Heart|ID:OE1w replicate 2|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq for myocardium specific hey2 overexpressing heart at 1 week post 4 HT treatments  rep2", "OE1w replicate 2", "OE1w replicate 2", "RNA Seq for myocardium specific hey2 overexpressing heart at 1 week post 4 HT treatments  replicate 2", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PCR", "PAIRED", "BGISEQ", "BGISEQ-500", null, "SRP444408", null, null, "OE1w2_2.fq.gz OE1w2_1.fq.gz", "fastq fastq", 6742724700.0, 22475749.0, "OE1w2 1.fq.gz", "0:150 1:150", "A:1802906309;C:1568304698;G:1553625077;T:1817795677;N:92939", 150, 150, null, null, 1802906309, 1568304698, 1553625077, 1817795677, 92939, "SRX20708346", "SRS18001291", "SRA1657339", "East China Normal University|School of Life Sciences", "East China Normal University", 2, 0.93354, 0.91844, 0.06989, 0.0686, 0.74665, 0.74888, 0.52723, 0.52812, 150, 150, "B", "B", "biological fallback assumption", "bgi", "bgi", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2023-06-16", "Juvenile", "Juvenile", "Heart", "Cardiovascular System"]], "truncated": false, "filtered_table_rows_count": 25, "expanded_columns": [], "expandable_columns": [], "columns": ["rowid", "run.accession", "experiment.accession", "sample.accession", "study.accession", "bioproject", "study.title", "study.alias", "study.type", "study.abstract", "study.attributes", "study.PMIDs", "sample.description", "sample.title", "sample.alias", "sample.centername", "sample.attributes", "GEOsample.title", "GEOsample.dataprocessing", "GEOsample.source", "GEOsample.treatmentprotocol", "GEOsample.extractprotocol", "GEOsample.growthprotocol", "GEOsample.characteristics", "GEOsample.accession", "experiment.title", "experiment.alias", "experiment.library_name", "experiment.design_description", "experiment.library_construction_protocol", "experiment.attributes", "experiment.library_strategy", "experiment.library_source", "experiment.library_selection", "experiment.library_layout", "experiment.platform", "experiment.instrument_model", "experiment.spot_descriptor", "experiment.study_ref", "run.title", "run.attributes", "run.filename", "run.semantic_name", "run.total_bases", "run.total_spots", "run.alias", "run.read_lengths", "run.base_counts", "run.r1_length", "run.r2_length", "run.r3_length", "run.r4_length", "run.Acount", "run.Ccount", "run.Gcount", "run.Tcount", "run.Ncount", "run.experiment", "run.pool_member", "submission.accession", "submission.srasource", "submission.bioprojectsource", "seqdetective.n_mates", "seqdetective.mapping_rate.mate1", "seqdetective.mapping_rate.mate2", "seqdetective.nofeature_rate.mate1", "seqdetective.nofeature_rate.mate2", "seqdetective.sparsity.mate1", "seqdetective.sparsity.mate2", "seqdetective.pos_strand_rate.mate1", "seqdetective.pos_strand_rate.mate2", "seqdetective.readlen.mate1", "seqdetective.readlen.mate2", "seqdetective.judgement.mate1", "seqdetective.judgement.mate2", "seqdetective.judgement.reason", "platform_family", "instrument_generation", "read_bias", "selection_class", "prep_kit", "sc_or_bulk", "tech_class", "technology", "tech_variant", "submission.bioprojectsource.country", "earliest_date", "devstage_curation", "devstage_curation_coarse", "tissue_curation", "tissue_curation_coarse"], "primary_keys": [], "units": {}, "query": {"sql": "select rowid, [run.accession], [experiment.accession], [sample.accession], [study.accession], bioproject, [study.title], [study.alias], [study.type], [study.abstract], [study.attributes], [study.PMIDs], [sample.description], [sample.title], [sample.alias], [sample.centername], [sample.attributes], [GEOsample.title], [GEOsample.dataprocessing], [GEOsample.source], [GEOsample.treatmentprotocol], [GEOsample.extractprotocol], [GEOsample.growthprotocol], [GEOsample.characteristics], [GEOsample.accession], [experiment.title], [experiment.alias], [experiment.library_name], [experiment.design_description], [experiment.library_construction_protocol], [experiment.attributes], [experiment.library_strategy], [experiment.library_source], [experiment.library_selection], [experiment.library_layout], [experiment.platform], [experiment.instrument_model], [experiment.spot_descriptor], [experiment.study_ref], [run.title], [run.attributes], [run.filename], [run.semantic_name], [run.total_bases], [run.total_spots], [run.alias], [run.read_lengths], [run.base_counts], [run.r1_length], [run.r2_length], [run.r3_length], [run.r4_length], [run.Acount], [run.Ccount], [run.Gcount], [run.Tcount], [run.Ncount], [run.experiment], [run.pool_member], [submission.accession], [submission.srasource], [submission.bioprojectsource], [seqdetective.n_mates], [seqdetective.mapping_rate.mate1], [seqdetective.mapping_rate.mate2], [seqdetective.nofeature_rate.mate1], [seqdetective.nofeature_rate.mate2], [seqdetective.sparsity.mate1], [seqdetective.sparsity.mate2], [seqdetective.pos_strand_rate.mate1], [seqdetective.pos_strand_rate.mate2], [seqdetective.readlen.mate1], [seqdetective.readlen.mate2], [seqdetective.judgement.mate1], [seqdetective.judgement.mate2], [seqdetective.judgement.reason], platform_family, instrument_generation, read_bias, selection_class, prep_kit, sc_or_bulk, tech_class, technology, tech_variant, [submission.bioprojectsource.country], earliest_date, devstage_curation, devstage_curation_coarse, tissue_curation, tissue_curation_coarse from run_metadata where \"devstage_curation_coarse\" = :p0 and \"tissue_curation_coarse\" = :p1 order by rowid limit 101", "params": {"p0": "Juvenile", "p1": "Cardiovascular System"}}, "facet_results": {"experiment.library_strategy": {"name": "experiment.library_strategy", "type": "column", "hideable": false, "toggle_url": "/metadata/run_metadata.json?devstage_curation_coarse=Juvenile&tissue_curation_coarse=Cardiovascular+System", "results": [{"value": "RNA-Seq", "label": "RNA-Seq", "count": 25, "toggle_url": "http://metadata.rnaquarium.org/metadata/run_metadata.json?devstage_curation_coarse=Juvenile&tissue_curation_coarse=Cardiovascular+System&experiment.library_strategy=RNA-Seq", "selected": false}], "truncated": false}, "experiment.library_source": {"name": "experiment.library_source", "type": "column", "hideable": false, "toggle_url": "/metadata/run_metadata.json?devstage_curation_coarse=Juvenile&tissue_curation_coarse=Cardiovascular+System", "results": [{"value": "TRANSCRIPTOMIC SINGLE CELL", "label": "TRANSCRIPTOMIC SINGLE CELL", "count": 13, "toggle_url": "http://metadata.rnaquarium.org/metadata/run_metadata.json?devstage_curation_coarse=Juvenile&tissue_curation_coarse=Cardiovascular+System&experiment.library_source=TRANSCRIPTOMIC+SINGLE+CELL", "selected": false}, {"value": "TRANSCRIPTOMIC", "label": "TRANSCRIPTOMIC", "count": 12, "toggle_url": "http://metadata.rnaquarium.org/metadata/run_metadata.json?devstage_curation_coarse=Juvenile&tissue_curation_coarse=Cardiovascular+System&experiment.library_source=TRANSCRIPTOMIC", "selected": false}], "truncated": false}, "experiment.library_selection": {"name": "experiment.library_selection", "type": "column", "hideable": false, "toggle_url": "/metadata/run_metadata.json?devstage_curation_coarse=Juvenile&tissue_curation_coarse=Cardiovascular+System", "results": [{"value": "cDNA", "label": "cDNA", "count": 13, "toggle_url": "http://metadata.rnaquarium.org/metadata/run_metadata.json?devstage_curation_coarse=Juvenile&tissue_curation_coarse=Cardiovascular+System&experiment.library_selection=cDNA", "selected": false}, {"value": "PCR", "label": "PCR", "count": 6, "toggle_url": "http://metadata.rnaquarium.org/metadata/run_metadata.json?devstage_curation_coarse=Juvenile&tissue_curation_coarse=Cardiovascular+System&experiment.library_selection=PCR", "selected": false}, {"value": "PolyA", "label": "PolyA", "count": 6, "toggle_url": "http://metadata.rnaquarium.org/metadata/run_metadata.json?devstage_curation_coarse=Juvenile&tissue_curation_coarse=Cardiovascular+System&experiment.library_selection=PolyA", "selected": false}], "truncated": false}, "experiment.library_layout": {"name": "experiment.library_layout", "type": "column", "hideable": false, "toggle_url": "/metadata/run_metadata.json?devstage_curation_coarse=Juvenile&tissue_curation_coarse=Cardiovascular+System", "results": [{"value": "PAIRED", "label": "PAIRED", "count": 25, "toggle_url": "http://metadata.rnaquarium.org/metadata/run_metadata.json?devstage_curation_coarse=Juvenile&tissue_curation_coarse=Cardiovascular+System&experiment.library_layout=PAIRED", "selected": false}], "truncated": false}, "experiment.platform": {"name": "experiment.platform", "type": "column", "hideable": false, "toggle_url": "/metadata/run_metadata.json?devstage_curation_coarse=Juvenile&tissue_curation_coarse=Cardiovascular+System", "results": [{"value": "ILLUMINA", "label": "ILLUMINA", "count": 19, "toggle_url": "http://metadata.rnaquarium.org/metadata/run_metadata.json?devstage_curation_coarse=Juvenile&tissue_curation_coarse=Cardiovascular+System&experiment.platform=ILLUMINA", "selected": false}, {"value": "BGISEQ", "label": "BGISEQ", "count": 6, "toggle_url": "http://metadata.rnaquarium.org/metadata/run_metadata.json?devstage_curation_coarse=Juvenile&tissue_curation_coarse=Cardiovascular+System&experiment.platform=BGISEQ", "selected": false}], "truncated": false}, "devstage_curation_coarse": {"name": "devstage_curation_coarse", "type": "column", "hideable": false, "toggle_url": "/metadata/run_metadata.json?devstage_curation_coarse=Juvenile&tissue_curation_coarse=Cardiovascular+System", "results": [{"value": "Juvenile", "label": "Juvenile", "count": 25, "toggle_url": "http://metadata.rnaquarium.org/metadata/run_metadata.json?tissue_curation_coarse=Cardiovascular+System", "selected": true}], "truncated": false}, "devstage_curation": {"name": "devstage_curation", "type": "column", "hideable": false, "toggle_url": "/metadata/run_metadata.json?devstage_curation_coarse=Juvenile&tissue_curation_coarse=Cardiovascular+System", "results": [{"value": "Juvenile", "label": "Juvenile", "count": 25, "toggle_url": "http://metadata.rnaquarium.org/metadata/run_metadata.json?devstage_curation_coarse=Juvenile&tissue_curation_coarse=Cardiovascular+System&devstage_curation=Juvenile", "selected": false}], "truncated": false}, "tissue_curation_coarse": {"name": "tissue_curation_coarse", "type": "column", "hideable": false, "toggle_url": "/metadata/run_metadata.json?devstage_curation_coarse=Juvenile&tissue_curation_coarse=Cardiovascular+System", "results": [{"value": "Cardiovascular System", "label": "Cardiovascular System", "count": 25, "toggle_url": "http://metadata.rnaquarium.org/metadata/run_metadata.json?devstage_curation_coarse=Juvenile", "selected": true}], "truncated": false}, "tissue_curation": {"name": "tissue_curation", "type": "column", "hideable": false, "toggle_url": "/metadata/run_metadata.json?devstage_curation_coarse=Juvenile&tissue_curation_coarse=Cardiovascular+System", "results": [{"value": "Heart", "label": "Heart", "count": 19, "toggle_url": "http://metadata.rnaquarium.org/metadata/run_metadata.json?devstage_curation_coarse=Juvenile&tissue_curation_coarse=Cardiovascular+System&tissue_curation=Heart", "selected": false}, {"value": "Lymphatic System", "label": "Lymphatic System", "count": 6, "toggle_url": "http://metadata.rnaquarium.org/metadata/run_metadata.json?devstage_curation_coarse=Juvenile&tissue_curation_coarse=Cardiovascular+System&tissue_curation=Lymphatic+System", "selected": false}], "truncated": false}, "technology": {"name": "technology", "type": "column", "hideable": false, "toggle_url": "/metadata/run_metadata.json?devstage_curation_coarse=Juvenile&tissue_curation_coarse=Cardiovascular+System", "results": [{"value": "10x", "label": "10x", "count": 13, "toggle_url": "http://metadata.rnaquarium.org/metadata/run_metadata.json?devstage_curation_coarse=Juvenile&tissue_curation_coarse=Cardiovascular+System&technology=10x", "selected": false}, {"value": "unknown", "label": "unknown", "count": 12, "toggle_url": "http://metadata.rnaquarium.org/metadata/run_metadata.json?devstage_curation_coarse=Juvenile&tissue_curation_coarse=Cardiovascular+System&technology=unknown", "selected": false}], "truncated": false}}, "suggested_facets": [], "next": null, "next_url": null, "private": false, "allow_execute_sql": true, "query_ms": 103.10408799705328}