{"database": "metadata", "table": "run_metadata", "is_view": false, "human_description_en": "where devstage_curation = \"Zygote\" and tissue_curation = \"Multi-tissue\"", "rows": [[175, "DRR084197", "DRX078028", "DRS086522", "DRP004758", "PRJDB5490", "Fine selection of up regulated genes duirng ovulation by in vivo induction of oocyte maturation and ovulation in zebrafish", "DRP004758", "Other", "Two essential processes  oocyte maturation and ovulation  before oocytes become fertilizable that are independently induced but co operatively proceeded at the final step in oogenesis. Eventhough these two processes are induced by same maturation inducing steroid  17  20 beta dihydroxy 4 pregnen 3 one 17  20 beta DHP  in teleost  the receptor for each pathway is suggested to be different and thus signal transduction pathways are different. While much progresses achieved on the molecular mechanisms for induction of oocyte maturation  the mechanisms to induce ovulation is under elucidation. Previously we established the procedure that can make it possible to prepare the ovarian tissue which contains oocyte maturation induced oocytes in vivo. In the same way  ovulation can be induced in alive zebrafish. Thus it became possible to select the genes up regulated according to ovulation by compare the gene expression between maturation inducing genes in matured oocytes and both maturation and ovulation inducing genes in ovulated eggs. In vivo bioassay has been applied to prepare maturated and ovulated ovarian samples. Specifically up regulated genes to induce ovulation will be selected by RNA seq analysis. The mRNA abundance of highly up regulated genes will be confirmed by q PCR analysis. By this project  ovulation inducing genes will be selected and its roles in induction of ovulation will be addressed in the future.", null, null, "natural paring early sample", "zebrafish ovary isolated from adult fish natural paring oocyte maturation. 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A 10 base indexing sequence TCGAAGTGAT is bases 13 to 22 of read 1 followed by polyT.|sample name:stI oocytes TC9 WT F sc 1951842|scientific name:Danio rerio|strain:AB", null, null, null, null, null, null, null, null, "Illumina HiSeq 2000 paired end sequencing", "SC EXP 13129 8#9", "10325318", "Illumina sequencing of library 10325318  constructed from sample accession ERS460539 for study accession ERP003932.  This is part of an Illumina multiplexed sequencing run 13129 8.  This submission includes reads tagged with the sequence TCGAAGTG.", "qPCR only", null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina HiSeq 2000", null, "ERP003932", "Illumina HiSeq 2000 paired end sequencing", "ENA FIRST PUBLIC:2014 09 16|ENA LAST UPDATE:2018 11 16", "13129_8#9.cram", "cram", 2109545984.0, 16480828.0, "SC RUN 13129 8#9", "0:53 1:75", "A:539694535;C:346663352;G:388843341;T:829045236;N:5299520", 53, 75, null, null, 539694535, 346663352, 388843341, 829045236, 5299520, "ERX557514", "ERS460539", "ERA358077", "SC", "Wellcome Sanger Institute", 2, 0.39712, 0.80906, 0.2298, 0.0288, 0.97575, 0.82292, 0.20258, 0.50014, 53, 75, "T", "B", "mate1 technical by mapping diff", "illumina", "hiseq_era", "3prime", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United Kingdom", "2014-09-16", "Zygote", "Embryo", "Multi-tissue", "Multi-system"], [10189, "ERR600684", "ERX557513", "ERS460538", "ERP003932", "PRJEB4632", "Zebrafish oocyte maturation", "Zebrafish_oocyte_maturation-sc-2013-09-24T12:03:04Z-2777", "Transcriptome Analysis", "The data within this study has been generated to study zebrafish oocyte maturation.", null, null, null, null, "SAMEA2537629", "SC", "ArrayExpress Genotype:buc|ArrayExpress OrganismPart:ovaries|ArrayExpress Sex:female|ArrayExpress Species:Danio rerio|ENA FIRST PUBLIC:2014 09 16T09:23:11Z|ENA LAST UPDATE:2018 03 08T17:41:18Z|External Id:SAMEA2537629|INSDC center name:SC|INSDC first public:2014 09 16T09:23:11Z|INSDC last update:2018 03 08T17:41:18Z|INSDC status:public|Submitter Id:stI oocytes TC8 buc 4 sc 1951841|common name:zebrafish|sample description:3 prime end enriched mRNA from oocytes. A 10 base indexing sequence TTCCATTGAT is bases 13 to 22 of read 1 followed by polyT.|sample name:stI oocytes TC8 buc 4 sc 1951841|scientific name:Danio rerio|strain:AB", null, null, null, null, null, null, null, null, "Illumina HiSeq 2000 paired end sequencing", "SC EXP 13129 8#8", "10325317", "Illumina sequencing of library 10325317  constructed from sample accession ERS460538 for study accession ERP003932.  This is part of an Illumina multiplexed sequencing run 13129 8.  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A 10 base indexing sequence TGTGGTTGAT is bases 13 to 22 of read 1 followed by polyT.|sample name:stI oocytes TC6 buc 2 sc 1951839|scientific name:Danio rerio|strain:AB", null, null, null, null, null, null, null, null, "Illumina HiSeq 2000 paired end sequencing", "SC EXP 13129 8#6", "10325315", "Illumina sequencing of library 10325315  constructed from sample accession ERS460536 for study accession ERP003932.  This is part of an Illumina multiplexed sequencing run 13129 8.  This submission includes reads tagged with the sequence TGTGGTTG.", "qPCR only", null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina HiSeq 2000", null, "ERP003932", "Illumina HiSeq 2000 paired end sequencing", "ENA FIRST PUBLIC:2014 09 16|ENA LAST UPDATE:2018 11 16", "13129_8#6.cram", "cram", 2044001792.0, 15968764.0, "SC RUN 13129 8#6", "0:53 1:75", "A:525134102;C:334554692;G:372135205;T:806953076;N:5224717", 53, 75, null, null, 525134102, 334554692, 372135205, 806953076, 5224717, "ERX557511", "ERS460536", "ERA358077", "SC", "Wellcome Sanger Institute", 2, 0.32466, 0.83888, 0.17696, 0.03057, 0.97451, 0.82938, 0.2392, 0.48605, 53, 75, "T", "B", "mate1 technical by mapping diff", "illumina", "hiseq_era", "3prime", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United Kingdom", "2014-09-16", "Zygote", "Embryo", "Multi-tissue", "Multi-system"], [10192, "ERR600681", "ERX557510", "ERS460535", "ERP003932", "PRJEB4632", "Zebrafish oocyte maturation", "Zebrafish_oocyte_maturation-sc-2013-09-24T12:03:04Z-2777", "Transcriptome Analysis", "The data within this study has been generated to study zebrafish oocyte maturation.", null, null, null, null, "SAMEA2537626", "SC", "ArrayExpress Genotype:buc|ArrayExpress OrganismPart:ovaries|ArrayExpress Sex:female|ArrayExpress Species:Danio rerio|ENA FIRST PUBLIC:2014 09 16T09:23:11Z|ENA LAST UPDATE:2018 03 08T17:41:18Z|External Id:SAMEA2537626|INSDC center name:SC|INSDC first public:2014 09 16T09:23:11Z|INSDC last update:2018 03 08T17:41:18Z|INSDC status:public|Submitter Id:stI oocytes TC5 buc 1 sc 1951838|common name:zebrafish|sample description:3 prime end enriched mRNA from oocytes. 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This submission includes reads tagged with the sequence TCCTCAAT.", "qPCR only", null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina HiSeq 2000", null, "ERP003932", "Illumina HiSeq 2000 paired end sequencing", "ENA FIRST PUBLIC:2014 09 16|ENA LAST UPDATE:2018 11 16", "13129_8#5.cram", "cram", 2936817024.0, 22943883.0, "SC RUN 13129 8#5", "0:53 1:75", "A:708314449;C:473731341;G:531306146;T:1215982569;N:7482519", 53, 75, null, null, 708314449, 473731341, 531306146, 1215982569, 7482519, "ERX557510", "ERS460535", "ERA358077", "SC", "Wellcome Sanger Institute", 2, 0.38759, 0.88578, 0.22255, 0.03034, 0.9707, 0.80984, 0.2075, 0.47267, 53, 75, "T", "B", "mate1 technical by mapping diff", "illumina", "hiseq_era", "3prime", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United Kingdom", "2014-09-16", "Zygote", "Embryo", "Multi-tissue", "Multi-system"], [10193, "ERR600680", "ERX557509", "ERS460534", "ERP003932", "PRJEB4632", "Zebrafish oocyte maturation", "Zebrafish_oocyte_maturation-sc-2013-09-24T12:03:04Z-2777", "Transcriptome Analysis", "The data within this study has been generated to study zebrafish oocyte maturation.", null, null, null, null, "SAMEA2537625", "SC", "ArrayExpress Genotype:Tgbuc:del80|ArrayExpress OrganismPart:ovaries|ArrayExpress Sex:female|ArrayExpress Species:Danio rerio|ENA FIRST PUBLIC:2014 09 16T09:23:11Z|ENA LAST UPDATE:2018 03 08T17:57:23Z|External Id:SAMEA2537625|INSDC center name:SC|INSDC first public:2014 09 16T09:23:11Z|INSDC last update:2018 03 08T17:57:23Z|INSDC status:public|Submitter Id:stI oocytes TC4 Tg 1 sc 1951837|common name:zebrafish|sample description:3 prime end enriched mRNA from oocytes. 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This submission includes reads tagged with the sequence TACAGGAT.", "qPCR only", null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina HiSeq 2000", null, "ERP003932", "Illumina HiSeq 2000 paired end sequencing", "ENA FIRST PUBLIC:2014 09 16|ENA LAST UPDATE:2018 11 16", "13129_8#4.cram", "cram", 2384869248.0, 18631791.0, "SC RUN 13129 8#4", "0:53 1:75", "A:579389373;C:378753292;G:429075103;T:991563565;N:6087915", 53, 75, null, null, 579389373, 378753292, 429075103, 991563565, 6087915, "ERX557509", "ERS460534", "ERA358077", "SC", "Wellcome Sanger Institute", 2, 0.48038, 0.84929, 0.28951, 0.03671, 0.97788, 0.81249, 0.13153, 0.4899, 53, 75, "T", "B", "mate1 technical by mapping diff", "illumina", "hiseq_era", "3prime", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United Kingdom", "2014-09-16", "Zygote", "Embryo", "Multi-tissue", "Multi-system"], [10194, "ERR600679", "ERX557508", "ERS460533", "ERP003932", "PRJEB4632", "Zebrafish oocyte maturation", "Zebrafish_oocyte_maturation-sc-2013-09-24T12:03:04Z-2777", "Transcriptome Analysis", "The data within this study has been generated to study zebrafish oocyte maturation.", null, null, null, null, "SAMEA2537624", "SC", "ArrayExpress Genotype:WT|ArrayExpress OrganismPart:ovaries|ArrayExpress Sex:female|ArrayExpress Species:Danio rerio|ENA FIRST PUBLIC:2014 09 16T09:23:11Z|ENA LAST UPDATE:2018 03 08T17:42:28Z|External Id:SAMEA2537624|INSDC center name:SC|INSDC first public:2014 09 16T09:23:11Z|INSDC last update:2018 03 08T17:42:28Z|INSDC status:public|Submitter Id:stI oocytes TC3 WT 3 sc 1951836|common name:zebrafish|sample description:3 prime end enriched mRNA from oocytes. A 10 base indexing sequence TAGTGACTAT is bases 13 to 22 of read 1 followed by polyT.|sample name:stI oocytes TC3 WT 3 sc 1951836|scientific name:Danio rerio|strain:AB", null, null, null, null, null, null, null, null, "Illumina HiSeq 2000 paired end sequencing", "SC EXP 13129 8#3", "10325312", "Illumina sequencing of library 10325312  constructed from sample accession ERS460533 for study accession ERP003932.  This is part of an Illumina multiplexed sequencing run 13129 8.  This submission includes reads tagged with the sequence TAGTGACT.", "qPCR only", null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina HiSeq 2000", null, "ERP003932", "Illumina HiSeq 2000 paired end sequencing", "ENA FIRST PUBLIC:2014 09 16|ENA LAST UPDATE:2018 11 16", "13129_8#3.cram", "cram", 2105031424.0, 16445558.0, "SC RUN 13129 8#3", "0:53 1:75", "A:508334707;C:332423893;G:374815782;T:884154321;N:5302721", 53, 75, null, null, 508334707, 332423893, 374815782, 884154321, 5302721, "ERX557508", "ERS460533", "ERA358077", "SC", "Wellcome Sanger Institute", 2, 0.50684, 0.84751, 0.30664, 0.04222, 0.97853, 0.81343, 0.11336, 0.49639, 53, 75, "T", "B", "mate1 technical by mapping diff", "illumina", "hiseq_era", "3prime", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United Kingdom", "2014-09-16", "Zygote", "Embryo", "Multi-tissue", "Multi-system"], [10195, "ERR600678", "ERX557507", "ERS460532", "ERP003932", "PRJEB4632", "Zebrafish oocyte maturation", "Zebrafish_oocyte_maturation-sc-2013-09-24T12:03:04Z-2777", "Transcriptome Analysis", "The data within this study has been generated to study zebrafish oocyte maturation.", null, null, null, null, "SAMEA2537623", "SC", "ArrayExpress Genotype:WT|ArrayExpress OrganismPart:ovaries|ArrayExpress Sex:female|ArrayExpress Species:Danio rerio|ENA FIRST PUBLIC:2014 09 16T09:23:11Z|ENA LAST UPDATE:2018 03 08T17:41:18Z|External Id:SAMEA2537623|INSDC center name:SC|INSDC first public:2014 09 16T09:23:11Z|INSDC last update:2018 03 08T17:41:18Z|INSDC status:public|Submitter Id:stI oocytes TC2 WT 2 sc 1951835|common name:zebrafish|sample description:3 prime end enriched mRNA from oocytes. A 10 base indexing sequence TTCCTGCTAT is bases 13 to 22 of read 1 followed by polyT.|sample name:stI oocytes TC2 WT 2 sc 1951835|scientific name:Danio rerio|strain:AB", null, null, null, null, null, null, null, null, "Illumina HiSeq 2000 paired end sequencing", "SC EXP 13129 8#2", "10325311", "Illumina sequencing of library 10325311  constructed from sample accession ERS460532 for study accession ERP003932.  This is part of an Illumina multiplexed sequencing run 13129 8.  This submission includes reads tagged with the sequence TTCCTGCT.", "qPCR only", null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina HiSeq 2000", null, "ERP003932", "Illumina HiSeq 2000 paired end sequencing", "ENA FIRST PUBLIC:2014 09 16|ENA LAST UPDATE:2018 11 16", "13129_8#2.cram", "cram", 1616469504.0, 12628668.0, "SC RUN 13129 8#2", "0:53 1:75", "A:415492342;C:254209235;G:288719848;T:653979238;N:4068841", 53, 75, null, null, 415492342, 254209235, 288719848, 653979238, 4068841, "ERX557507", "ERS460532", "ERA358077", "SC", "Wellcome Sanger Institute", 2, 0.44661, 0.81908, 0.25181, 0.0449, 0.96469, 0.80306, 0.22578, 0.49499, 53, 75, "T", "B", "mate1 technical by mapping diff", "illumina", "hiseq_era", "3prime", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United Kingdom", "2014-09-16", "Zygote", "Embryo", "Multi-tissue", "Multi-system"], [10196, "ERR600677", "ERX557506", "ERS460531", "ERP003932", "PRJEB4632", "Zebrafish oocyte maturation", "Zebrafish_oocyte_maturation-sc-2013-09-24T12:03:04Z-2777", "Transcriptome Analysis", "The data within this study has been generated to study zebrafish oocyte maturation.", null, null, null, null, "SAMEA2537622", "SC", "ArrayExpress Genotype:WT|ArrayExpress OrganismPart:ovaries|ArrayExpress Sex:female|ArrayExpress Species:Danio rerio|ENA FIRST PUBLIC:2014 09 16T09:23:11Z|ENA LAST UPDATE:2018 03 08T17:57:22Z|External Id:SAMEA2537622|INSDC center name:SC|INSDC first public:2014 09 16T09:23:11Z|INSDC last update:2018 03 08T17:57:22Z|INSDC status:public|Submitter Id:stI oocytes TC1 WT 1 sc 1951834|common name:zebrafish|sample description:3 prime end enriched mRNA from oocytes. A 10 base indexing sequence TGCGATCTAT is bases 13 to 22 of read 1 followed by polyT.|sample name:stI oocytes TC1 WT 1 sc 1951834|scientific name:Danio rerio|strain:AB", null, null, null, null, null, null, null, null, "Illumina HiSeq 2000 paired end sequencing", "SC EXP 13129 8#1", "10325310", "Illumina sequencing of library 10325310  constructed from sample accession ERS460531 for study accession ERP003932.  This is part of an Illumina multiplexed sequencing run 13129 8.  This submission includes reads tagged with the sequence TGCGATCT.", "qPCR only", null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina HiSeq 2000", null, "ERP003932", "Illumina HiSeq 2000 paired end sequencing", "ENA FIRST PUBLIC:2014 09 16|ENA LAST UPDATE:2018 11 16", "13129_8#1.cram", "cram", 1741512832.0, 13605569.0, "SC RUN 13129 8#1", "0:53 1:75", "A:460247357;C:265029516;G:295203994;T:716636989;N:4394976", 53, 75, null, null, 460247357, 265029516, 295203994, 716636989, 4394976, "ERX557506", "ERS460531", "ERA358077", "SC", "Wellcome Sanger Institute", 2, 0.36591, 0.79177, 0.21838, 0.03785, 0.97569, 0.81095, 0.16098, 0.49496, 53, 75, "T", "B", "mate1 technical by mapping diff", "illumina", "hiseq_era", "3prime", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United Kingdom", "2014-09-16", "Zygote", "Embryo", "Multi-tissue", "Multi-system"], [10197, "ERR596433", "ERX553396", "ERS462797", "ERP003932", "PRJEB4632", "Zebrafish oocyte maturation", "Zebrafish_oocyte_maturation-sc-2013-09-24T12:03:04Z-2777", "Transcriptome Analysis", "The data within this study has been generated to study zebrafish oocyte maturation.", null, null, null, null, "SAMEA2547987", "SC", "ArrayExpress Genotype:WT|ArrayExpress OrganismPart:ovaries|ArrayExpress Sex:female|ArrayExpress Species:Danio rerio|ENA FIRST PUBLIC:2014 09 09T09:35:46Z|ENA LAST UPDATE:2018 03 08T17:41:51Z|External Id:SAMEA2547987|INSDC center name:SC|INSDC first public:2014 09 09T09:35:46Z|INSDC last update:2018 03 08T17:41:51Z|INSDC status:public|Submitter Id:stI oocytes Fraction9 WT F sc 1971686|common name:zebrafish|sample description:Oocyte RNA fraction.|sample name:stI oocytes Fraction9 WT F sc 1971686|scientific name:Danio rerio|strain:AB", null, null, null, null, null, null, null, null, "Illumina MiSeq paired end sequencing", "SC EXP 13147 1#9", "10347698", "Illumina sequencing of library 10347698  constructed from sample accession ERS462797 for study accession ERP003932.  This is part of an Illumina multiplexed sequencing run 13147 1.  This submission includes reads tagged with the sequence GATCAG.", "RNA seq dUTP", null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina MiSeq", null, "ERP003932", "Illumina MiSeq paired end sequencing", "ENA FIRST PUBLIC:2014 09 09|ENA LAST UPDATE:2018 11 16", "13147_1#9.cram", "cram", 552103200.0, 1840344.0, "SC RUN 13147 1#9", "0:150 1:150", "A:124691243;C:154385942;G:155440122;T:117585893;N:0", 150, 150, null, null, 124691243, 154385942, 155440122, 117585893, 0, "ERX553396", "ERS462797", "ERA356861", "SC", "Wellcome Sanger Institute", 2, 0.97349, 0.97184, 0.25562, 0.26367, 0.73754, 0.76534, 0.68526, 0.65497, 150, 150, "B", "B", "biological fallback assumption", "illumina", "miseq", "unknown", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United Kingdom", "2014-09-09", "Zygote", "Embryo", "Multi-tissue", "Multi-system"], [10198, "ERR596432", "ERX553395", "ERS462796", "ERP003932", "PRJEB4632", "Zebrafish oocyte maturation", "Zebrafish_oocyte_maturation-sc-2013-09-24T12:03:04Z-2777", "Transcriptome Analysis", "The data within this study has been generated to study zebrafish oocyte maturation.", null, null, null, null, "SAMEA2547986", "SC", "ArrayExpress Genotype:buc|ArrayExpress OrganismPart:ovaries|ArrayExpress Sex:female|ArrayExpress Species:Danio rerio|ENA FIRST PUBLIC:2014 09 09T09:35:46Z|ENA LAST UPDATE:2018 03 08T17:58:35Z|External Id:SAMEA2547986|INSDC center name:SC|INSDC first public:2014 09 09T09:35:46Z|INSDC last update:2018 03 08T17:58:35Z|INSDC status:public|Submitter Id:stI oocytes Fraction8 buc 4 sc 1971685|common name:zebrafish|sample description:Oocyte RNA fraction.|sample name:stI oocytes Fraction8 buc 4 sc 1971685|scientific name:Danio rerio|strain:AB", null, null, null, null, null, null, null, null, "Illumina MiSeq paired end sequencing", "SC EXP 13147 1#8", "10347697", "Illumina sequencing of library 10347697  constructed from sample accession ERS462796 for study accession ERP003932.  This is part of an Illumina multiplexed sequencing run 13147 1.  This submission includes reads tagged with the sequence ACTTGA.", "RNA seq dUTP", null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina MiSeq", null, "ERP003932", "Illumina MiSeq paired end sequencing", "ENA FIRST PUBLIC:2014 09 09|ENA LAST UPDATE:2018 11 16", "13147_1#8.cram", "cram", 625194300.0, 2083981.0, "SC RUN 13147 1#8", "0:150 1:150", "A:145587865;C:170554648;G:171453968;T:137597813;N:6", 150, 150, null, null, 145587865, 170554648, 171453968, 137597813, 6, "ERX553395", "ERS462796", "ERA356861", "SC", "Wellcome Sanger Institute", 2, 0.97066, 0.96788, 0.23219, 0.23823, 0.7205, 0.75623, 0.61734, 0.60233, 150, 150, "B", "B", "biological fallback assumption", "illumina", "miseq", "unknown", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United Kingdom", "2014-09-09", "Zygote", "Embryo", "Multi-tissue", "Multi-system"], [10199, "ERR596431", "ERX553394", "ERS462795", "ERP003932", "PRJEB4632", "Zebrafish oocyte maturation", "Zebrafish_oocyte_maturation-sc-2013-09-24T12:03:04Z-2777", "Transcriptome Analysis", "The data within this study has been generated to study zebrafish oocyte maturation.", null, null, null, null, "SAMEA2547985", "SC", "ArrayExpress Genotype:buc|ArrayExpress OrganismPart:ovaries|ArrayExpress Sex:female|ArrayExpress Species:Danio rerio|ENA FIRST PUBLIC:2014 09 09T09:35:46Z|ENA LAST UPDATE:2018 03 08T17:42:18Z|External Id:SAMEA2547985|INSDC center name:SC|INSDC first public:2014 09 09T09:35:46Z|INSDC last update:2018 03 08T17:42:18Z|INSDC status:public|Submitter Id:stI oocytes Fraction7 buc 3 sc 1971684|common name:zebrafish|sample description:Oocyte RNA fraction.|sample name:stI oocytes Fraction7 buc 3 sc 1971684|scientific name:Danio rerio|strain:AB", null, null, null, null, null, null, null, null, "Illumina MiSeq paired end sequencing", "SC EXP 13147 1#7", "10347696", "Illumina sequencing of library 10347696  constructed from sample accession ERS462795 for study accession ERP003932.  This is part of an Illumina multiplexed sequencing run 13147 1.  This submission includes reads tagged with the sequence CAGATC.", "RNA seq dUTP", null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina MiSeq", null, "ERP003932", "Illumina MiSeq paired end sequencing", "ENA FIRST PUBLIC:2014 09 09|ENA LAST UPDATE:2018 11 16", "13147_1#7.cram", "cram", 498083400.0, 1660278.0, "SC RUN 13147 1#7", "0:150 1:150", "A:127449944;C:127409242;G:133869886;T:109354327;N:1", 150, 150, null, null, 127449944, 127409242, 133869886, 109354327, 1, "ERX553394", "ERS462795", "ERA356861", "SC", "Wellcome Sanger Institute", 2, 0.94952, 0.95502, 0.14913, 0.14846, 0.73807, 0.74572, 0.54621, 0.54335, 150, 150, "B", "B", "biological fallback assumption", "illumina", "miseq", "unknown", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United Kingdom", "2014-09-09", "Zygote", "Embryo", "Multi-tissue", "Multi-system"], [10200, "ERR596430", "ERX553393", "ERS462794", "ERP003932", "PRJEB4632", "Zebrafish oocyte maturation", "Zebrafish_oocyte_maturation-sc-2013-09-24T12:03:04Z-2777", "Transcriptome Analysis", "The data within this study has been generated to study zebrafish oocyte maturation.", null, null, null, null, "SAMEA2547984", "SC", "ArrayExpress Genotype:buc|ArrayExpress OrganismPart:ovaries|ArrayExpress Sex:female|ArrayExpress Species:Danio rerio|ENA FIRST PUBLIC:2014 09 09T09:35:46Z|ENA LAST UPDATE:2018 03 08T17:41:51Z|External Id:SAMEA2547984|INSDC center name:SC|INSDC first public:2014 09 09T09:35:46Z|INSDC last update:2018 03 08T17:41:51Z|INSDC status:public|Submitter Id:stI oocytes Fraction6 buc 2 sc 1971683|common name:zebrafish|sample description:Oocyte RNA fraction.|sample name:stI oocytes Fraction6 buc 2 sc 1971683|scientific name:Danio rerio|strain:AB", null, null, null, null, null, null, null, null, "Illumina MiSeq paired end sequencing", "SC EXP 13147 1#6", "10347695", "Illumina sequencing of library 10347695  constructed from sample accession ERS462794 for study accession ERP003932.  This is part of an Illumina multiplexed sequencing run 13147 1.  This submission includes reads tagged with the sequence GCCAAT.", "RNA seq dUTP", null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina MiSeq", null, "ERP003932", "Illumina MiSeq paired end sequencing", "ENA FIRST PUBLIC:2014 09 09|ENA LAST UPDATE:2018 11 16", "13147_1#6.cram", "cram", 433626600.0, 1445422.0, "SC RUN 13147 1#6", "0:150 1:150", "A:113030099;C:110060861;G:116695113;T:93840526;N:1", 150, 150, null, null, 113030099, 110060861, 116695113, 93840526, 1, "ERX553393", "ERS462794", "ERA356861", "SC", "Wellcome Sanger Institute", 2, 0.94512, 0.95418, 0.13754, 0.13509, 0.76274, 0.77542, 0.54752, 0.54127, 150, 150, "B", "B", "biological fallback assumption", "illumina", "miseq", "unknown", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United Kingdom", "2014-09-09", "Zygote", "Embryo", "Multi-tissue", "Multi-system"], [10201, "ERR596429", "ERX553392", "ERS462793", "ERP003932", "PRJEB4632", "Zebrafish oocyte maturation", "Zebrafish_oocyte_maturation-sc-2013-09-24T12:03:04Z-2777", "Transcriptome Analysis", "The data within this study has been generated to study zebrafish oocyte maturation.", null, null, null, null, "SAMEA2547983", "SC", "ArrayExpress Genotype:buc|ArrayExpress OrganismPart:ovaries|ArrayExpress Sex:female|ArrayExpress Species:Danio rerio|ENA FIRST PUBLIC:2014 09 09T09:35:46Z|ENA LAST UPDATE:2018 03 08T17:58:34Z|External Id:SAMEA2547983|INSDC center name:SC|INSDC first public:2014 09 09T09:35:46Z|INSDC last update:2018 03 08T17:58:34Z|INSDC status:public|Submitter Id:stI oocytes Fraction5 buc 1 sc 1971682|common name:zebrafish|sample description:Oocyte RNA fraction.|sample name:stI oocytes Fraction5 buc 1 sc 1971682|scientific name:Danio rerio|strain:AB", null, null, null, null, null, null, null, null, "Illumina MiSeq paired end sequencing", "SC EXP 13147 1#5", "10347694", "Illumina sequencing of library 10347694  constructed from sample accession ERS462793 for study accession ERP003932.  This is part of an Illumina multiplexed sequencing run 13147 1.  This submission includes reads tagged with the sequence ACAGTG.", "RNA seq dUTP", null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina MiSeq", null, "ERP003932", "Illumina MiSeq paired end sequencing", "ENA FIRST PUBLIC:2014 09 09|ENA LAST UPDATE:2018 11 16", "13147_1#5.cram", "cram", 670533600.0, 2235112.0, "SC RUN 13147 1#5", "0:150 1:150", "A:152057213;C:184952145;G:191337018;T:142187224;N:0", 150, 150, null, null, 152057213, 184952145, 191337018, 142187224, 0, "ERX553392", "ERS462793", "ERA356861", "SC", "Wellcome Sanger Institute", 2, 0.95987, 0.95954, 0.20495, 0.20606, 0.73726, 0.7529, 0.61328, 0.61986, 150, 150, "B", "B", "biological fallback assumption", "illumina", "miseq", "unknown", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United Kingdom", "2014-09-09", "Zygote", "Embryo", "Multi-tissue", "Multi-system"], [10202, "ERR596428", "ERX553391", "ERS462792", "ERP003932", "PRJEB4632", "Zebrafish oocyte maturation", "Zebrafish_oocyte_maturation-sc-2013-09-24T12:03:04Z-2777", "Transcriptome Analysis", "The data within this study has been generated to study zebrafish oocyte maturation.", null, null, null, null, "SAMEA2547982", "SC", "ArrayExpress Genotype:Tgbuc:del80|ArrayExpress OrganismPart:ovaries|ArrayExpress Sex:female|ArrayExpress Species:Danio rerio|ENA FIRST PUBLIC:2014 09 09T09:35:46Z|ENA LAST UPDATE:2018 03 08T17:42:18Z|External Id:SAMEA2547982|INSDC center name:SC|INSDC first public:2014 09 09T09:35:46Z|INSDC last update:2018 03 08T17:42:18Z|INSDC status:public|Submitter Id:stI oocytes Fraction4 Tg 1 sc 1971681|common name:zebrafish|sample description:Oocyte RNA fraction.|sample name:stI oocytes Fraction4 Tg 1 sc 1971681|scientific name:Danio rerio|strain:AB", null, null, null, null, null, null, null, null, "Illumina MiSeq paired end sequencing", "SC EXP 13147 1#4", "10347693", "Illumina sequencing of library 10347693  constructed from sample accession ERS462792 for study accession ERP003932.  This is part of an Illumina multiplexed sequencing run 13147 1.  This submission includes reads tagged with the sequence TGACCA.", "RNA seq dUTP", null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina MiSeq", null, "ERP003932", "Illumina MiSeq paired end sequencing", "ENA FIRST PUBLIC:2014 09 09|ENA LAST UPDATE:2018 11 16", "13147_1#4.cram", "cram", 524202300.0, 1747341.0, "SC RUN 13147 1#4", "0:150 1:150", "A:117529161;C:147865152;G:153007969;T:105800018;N:0", 150, 150, null, null, 117529161, 147865152, 153007969, 105800018, 0, "ERX553391", "ERS462792", "ERA356861", "SC", "Wellcome Sanger Institute", 2, 0.95955, 0.96175, 0.21001, 0.20834, 0.76445, 0.78281, 0.70435, 0.68975, 150, 150, "B", "B", "biological fallback assumption", "illumina", "miseq", "unknown", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United Kingdom", "2014-09-09", "Zygote", "Embryo", "Multi-tissue", "Multi-system"], [10203, "ERR596427", "ERX553390", "ERS462791", "ERP003932", "PRJEB4632", "Zebrafish oocyte maturation", "Zebrafish_oocyte_maturation-sc-2013-09-24T12:03:04Z-2777", "Transcriptome Analysis", "The data within this study has been generated to study zebrafish oocyte maturation.", null, null, null, null, "SAMEA2547981", "SC", "ArrayExpress Genotype:WT|ArrayExpress OrganismPart:ovaries|ArrayExpress Sex:female|ArrayExpress Species:Danio rerio|ENA FIRST PUBLIC:2014 09 09T09:35:46Z|ENA LAST UPDATE:2018 03 08T17:41:51Z|External Id:SAMEA2547981|INSDC center name:SC|INSDC first public:2014 09 09T09:35:46Z|INSDC last update:2018 03 08T17:41:51Z|INSDC status:public|Submitter Id:stI oocytes Fraction3 WT 3 sc 1971680|common name:zebrafish|sample description:Oocyte RNA fraction.|sample name:stI oocytes Fraction3 WT 3 sc 1971680|scientific name:Danio rerio|strain:AB", null, null, null, null, null, null, null, null, "Illumina MiSeq paired end sequencing", "SC EXP 13147 1#3", "10347692", "Illumina sequencing of library 10347692  constructed from sample accession ERS462791 for study accession ERP003932.  This is part of an Illumina multiplexed sequencing run 13147 1.  This submission includes reads tagged with the sequence TTAGGC.", "RNA seq dUTP", null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina MiSeq", null, "ERP003932", "Illumina MiSeq paired end sequencing", "ENA FIRST PUBLIC:2014 09 09|ENA LAST UPDATE:2018 11 16", "13147_1#3.cram", "cram", 1142124300.0, 3807081.0, "SC RUN 13147 1#3", "0:150 1:150", "A:239327535;C:334933829;G:339423780;T:228439155;N:1", 150, 150, null, null, 239327535, 334933829, 339423780, 228439155, 1, "ERX553390", "ERS462791", "ERA356861", "SC", "Wellcome Sanger Institute", 2, 0.98041, 0.98099, 0.28225, 0.28585, 0.80273, 0.81012, 0.81256, 0.81436, 150, 150, "B", "B", "biological fallback assumption", "illumina", "miseq", "unknown", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United Kingdom", "2014-09-09", "Zygote", "Embryo", "Multi-tissue", "Multi-system"], [10204, "ERR596426", "ERX553389", "ERS462790", "ERP003932", "PRJEB4632", "Zebrafish oocyte maturation", "Zebrafish_oocyte_maturation-sc-2013-09-24T12:03:04Z-2777", "Transcriptome Analysis", "The data within this study has been generated to study zebrafish oocyte maturation.", null, null, null, null, "SAMEA2547980", "SC", "ArrayExpress Genotype:WT|ArrayExpress OrganismPart:ovaries|ArrayExpress Sex:female|ArrayExpress Species:Danio rerio|ENA FIRST PUBLIC:2014 09 09T09:35:46Z|ENA LAST UPDATE:2018 03 08T17:58:34Z|External Id:SAMEA2547980|INSDC center name:SC|INSDC first public:2014 09 09T09:35:46Z|INSDC last update:2018 03 08T17:58:34Z|INSDC status:public|Submitter Id:stI oocytes Fraction2 WT 2 sc 1971679|common name:zebrafish|sample description:Oocyte RNA fraction.|sample name:stI oocytes Fraction2 WT 2 sc 1971679|scientific name:Danio rerio|strain:AB", null, null, null, null, null, null, null, null, "Illumina MiSeq paired end sequencing", "SC EXP 13147 1#2", "10347691", "Illumina sequencing of library 10347691  constructed from sample accession ERS462790 for study accession ERP003932.  This is part of an Illumina multiplexed sequencing run 13147 1.  This submission includes reads tagged with the sequence CGATGT.", "RNA seq dUTP", null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina MiSeq", null, "ERP003932", "Illumina MiSeq paired end sequencing", "ENA FIRST PUBLIC:2014 09 09|ENA LAST UPDATE:2018 11 16", "13147_1#2.cram", "cram", 714969600.0, 2383232.0, "SC RUN 13147 1#2", "0:150 1:150", "A:151578902;C:207835645;G:217545196;T:138009856;N:1", 150, 150, null, null, 151578902, 207835645, 217545196, 138009856, 1, "ERX553389", "ERS462790", "ERA356861", "SC", "Wellcome Sanger Institute", 2, 0.97925, 0.981, 0.2548, 0.25496, 0.77447, 0.78462, 0.77035, 0.76498, 150, 150, "B", "B", "biological fallback assumption", "illumina", "miseq", "unknown", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United Kingdom", "2014-09-09", "Zygote", "Embryo", "Multi-tissue", "Multi-system"], [10205, "ERR596425", "ERX553388", "ERS462789", "ERP003932", "PRJEB4632", "Zebrafish oocyte maturation", "Zebrafish_oocyte_maturation-sc-2013-09-24T12:03:04Z-2777", "Transcriptome Analysis", "The data within this study has been generated to study zebrafish oocyte maturation.", null, null, null, null, "SAMEA2547979", "SC", "ArrayExpress Genotype:WT|ArrayExpress OrganismPart:ovaries|ArrayExpress Sex:female|ArrayExpress Species:Danio rerio|ENA FIRST PUBLIC:2014 09 09T09:35:46Z|ENA LAST UPDATE:2018 03 08T17:42:18Z|External Id:SAMEA2547979|INSDC center name:SC|INSDC first public:2014 09 09T09:35:46Z|INSDC last update:2018 03 08T17:42:18Z|INSDC status:public|Submitter Id:stI oocytes Fraction1 WT 1 sc 1971678|common name:zebrafish|sample description:Oocyte RNA fraction.|sample name:stI oocytes Fraction1 WT 1 sc 1971678|scientific name:Danio rerio|strain:AB", null, null, null, null, null, null, null, null, "Illumina MiSeq paired end sequencing", "SC EXP 13147 1#1", "10347690", "Illumina sequencing of library 10347690  constructed from sample accession ERS462789 for study accession ERP003932.  This is part of an Illumina multiplexed sequencing run 13147 1.  This submission includes reads tagged with the sequence ATCACG.", "RNA seq dUTP", null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina MiSeq", null, "ERP003932", "Illumina MiSeq paired end sequencing", "ENA FIRST PUBLIC:2014 09 09|ENA LAST UPDATE:2018 11 16", "13147_1#1.cram", "cram", 567682800.0, 1892276.0, "SC RUN 13147 1#1", "0:150 1:150", "A:133087363;C:153887726;G:158264603;T:122443107;N:1", 150, 150, null, null, 133087363, 153887726, 158264603, 122443107, 1, "ERX553388", "ERS462789", "ERA356861", "SC", "Wellcome Sanger Institute", 2, 0.94832, 0.94927, 0.19305, 0.19445, 0.72421, 0.73551, 0.59172, 0.60783, 150, 150, "B", "B", "biological fallback assumption", "illumina", "miseq", "unknown", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United Kingdom", "2014-09-09", "Zygote", "Embryo", "Multi-tissue", "Multi-system"]], "truncated": false, "filtered_table_rows_count": 20, "expanded_columns": [], "expandable_columns": [], "columns": 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"devstage_curation", "devstage_curation_coarse", "tissue_curation", "tissue_curation_coarse"], "primary_keys": [], "units": {}, "query": {"sql": "select rowid, [run.accession], [experiment.accession], [sample.accession], [study.accession], bioproject, [study.title], [study.alias], [study.type], [study.abstract], [study.attributes], [study.PMIDs], [sample.description], [sample.title], [sample.alias], [sample.centername], [sample.attributes], [GEOsample.title], [GEOsample.dataprocessing], [GEOsample.source], [GEOsample.treatmentprotocol], [GEOsample.extractprotocol], [GEOsample.growthprotocol], [GEOsample.characteristics], [GEOsample.accession], [experiment.title], [experiment.alias], [experiment.library_name], [experiment.design_description], [experiment.library_construction_protocol], [experiment.attributes], [experiment.library_strategy], [experiment.library_source], [experiment.library_selection], [experiment.library_layout], [experiment.platform], [experiment.instrument_model], 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prep_kit, sc_or_bulk, tech_class, technology, tech_variant, [submission.bioprojectsource.country], earliest_date, devstage_curation, devstage_curation_coarse, tissue_curation, tissue_curation_coarse from run_metadata where \"devstage_curation\" = :p0 and \"tissue_curation\" = :p1 order by rowid limit 101", "params": {"p0": "Zygote", "p1": "Multi-tissue"}}, "facet_results": {"experiment.library_strategy": {"name": "experiment.library_strategy", "type": "column", "hideable": false, "toggle_url": "/metadata/run_metadata.json?devstage_curation=Zygote&tissue_curation=Multi-tissue", "results": [{"value": "RNA-Seq", "label": "RNA-Seq", "count": 20, "toggle_url": "http://metadata.rnaquarium.org/metadata/run_metadata.json?devstage_curation=Zygote&tissue_curation=Multi-tissue&experiment.library_strategy=RNA-Seq", "selected": false}], "truncated": false}, "experiment.library_source": {"name": "experiment.library_source", "type": "column", "hideable": false, "toggle_url": 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