{"database": "metadata", "table": "run_metadata", "is_view": false, "human_description_en": "where devstage_curation = \"Zygote\" and experiment.platform = \"PACBIO_SMRT\"", "rows": [[28587, "SRR26395063", "SRX22100871", "SRS19165997", "SRP466518", "PRJNA1028258", "Zygotic activation of transposable elements during zebrafish early embryogenesis", "PRJNA1028258", "Other", "Here we leverage high quality long reads plus manual annotation to establish a high resolution landscape of TE activation and transcription at the levels of locus  transcript  and allele over zebrafish early embryonic development. Moreover  we reveal a previously unknown temporal trajectory and subcellular distribution of zygotic TE activation ZTA in zebrafish  where extensive variation exists among TE families  subfamilies  loci  transcripts and alleles with respect to evolutionary age.", null, "pubmed:40246845", null, null, "1 cell Iso seq", null, "strain:AB x India|age:0.5 hpf|dev stage:1cell|collection date:2018 12|geo loc name:China:Beijing|sex:N/A|tissue:embryo|source material identifier:LR 2|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "PacBio Iso seq of zebrafish: 1 cell", "DR 002", "DR 002", "Total RNA was isolated from each developmental stages of zebrafish embryos using TRIzolTM Reagent Invitrogen. RNA purity and concentration were assessed with the NanoPhotometer spectrophotometer IMPLEN  CA  USA and the Qubit RNA Assay Kit in the Qubit 3.0 Fluorometer Life Technologies  CA  USA. The RNA integrity number RIN was determined using the RNA Nano 6000 Assay Kit and Agilent Bioanalyzer 2100 system Agilent Technologies  CA  USA. RNA samples with a RIN  8 were used to synthesize cDNA with SMARTerPCR cDNA Synthesis Kit Takara Bio USA  Inc.  Mountain View  CA  USA. PCR amplification was performed using a KAPA HiFi PCR Kit Kapa Biosystems  Wilmington  MA  USA with the optimized number of cycles. Size selection of PCR products cDNA for each sample was applied using the BluePippin System: <3 kb and >3 kb. Subsequently  two cDNA libraries <3 kb  >3 kb were prepared using a SMRTbell Template Prep Kit 1.0 Pacific Biosciences  Menlo Park  CA  USA and sequenced on the PacBio sequel II platform.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "SINGLE", "PACBIO_SMRT", "Sequel", null, "SRP466518", null, null, "cell1_1.ccs.fq.gz cell1_2.ccs.fq.gz", "fastq fastq", 2076576705.0, 1053386.0, "cell1 1.ccs.fq.gz", "0:1971.34", "A:560055059;C:479061015;G:485136981;T:552323650;N:0", 1971, null, null, null, 560055059, 479061015, 485136981, 552323650, 0, "SRX22100871", "SRS19165997", "SRA1731898", "University of Michigan|Computational Medicine and Bioinformatics", "University of Michigan", 1, 0.44892, null, 0.00258, null, 0.90114, null, 0.50832, null, 32, null, "B", null, "usable mapping rate", "pacbio", "pacbio_modern", "full_length", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "United States", "2023-10-16", "Zygote", "Embryo", "Embryo Imprecise", "All anatomical structures"], [28588, "SRR26395064", "SRX22100870", "SRS19165996", "SRP466518", "PRJNA1028258", "Zygotic activation of transposable elements during zebrafish early embryogenesis", "PRJNA1028258", "Other", "Here we leverage high quality long reads plus manual annotation to establish a high resolution landscape of TE activation and transcription at the levels of locus  transcript  and allele over zebrafish early embryonic development. Moreover  we reveal a previously unknown temporal trajectory and subcellular distribution of zygotic TE activation ZTA in zebrafish  where extensive variation exists among TE families  subfamilies  loci  transcripts and alleles with respect to evolutionary age.", null, "pubmed:40246845", null, null, "fertilized egg Iso seq", null, "strain:AB x India|age:0 hpf|dev stage:fertilized egg|collection date:2018 12|geo loc name:China:Beijing|sex:N/A|tissue:embryo|source material identifier:LR 1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "PacBio Iso seq of zebrafish: fertilized egg", "DR 001", "DR 001", "Total RNA was isolated from each developmental stages of zebrafish embryos using TRIzolTM Reagent Invitrogen. RNA purity and concentration were assessed with the NanoPhotometer spectrophotometer IMPLEN  CA  USA and the Qubit RNA Assay Kit in the Qubit 3.0 Fluorometer Life Technologies  CA  USA. The RNA integrity number RIN was determined using the RNA Nano 6000 Assay Kit and Agilent Bioanalyzer 2100 system Agilent Technologies  CA  USA. RNA samples with a RIN  8 were used to synthesize cDNA with SMARTerPCR cDNA Synthesis Kit Takara Bio USA  Inc.  Mountain View  CA  USA. PCR amplification was performed using a KAPA HiFi PCR Kit Kapa Biosystems  Wilmington  MA  USA with the optimized number of cycles. Size selection of PCR products cDNA for each sample was applied using the BluePippin System: <3 kb and >3 kb. Subsequently  two cDNA libraries <3 kb  >3 kb were prepared using a SMRTbell Template Prep Kit 1.0 Pacific Biosciences  Menlo Park  CA  USA and sequenced on the PacBio sequel II platform.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "Oligo-dT", "SINGLE", "PACBIO_SMRT", "Sequel", null, "SRP466518", null, null, "cell0_1.ccs.fq.gz cell0_2.ccs.fq.gz cell0_3.ccs.fq.gz", "fastq fastq fastq", 3550605210.0, 2083545.0, "cell0 1.ccs.fq.gz", "0:1704.12", "A:990393181;C:795168216;G:861389798;T:903654015;N:0", 1704, null, null, null, 990393181, 795168216, 861389798, 903654015, 0, "SRX22100870", "SRS19165996", "SRA1731898", "University of Michigan|Computational Medicine and Bioinformatics", "University of Michigan", 1, 0.33296, null, 0.00275, null, 0.95548, null, 0.552, null, 1155, null, "T", null, "long read", "pacbio", "pacbio_modern", "full_length", "poly_a", "unknown", "bulk", "unknown", "unknown", null, "United States", "2023-10-16", "Zygote", "Embryo", "Embryo Imprecise", "All anatomical structures"], [34978, "SRR32588735", "SRX27895216", "SRS24266218", "SRP568323", "PRJNA1232602", "Telomere to telomere genome assemblies for commonly used zebrafish laboratory strains", "PRJNA1232602", "Other", "In this study  Iso Seq was performed on different zebrafish body organs  enabling a comprehensive view of organ specific transcriptomes. High quality PacBio Iso Seq long reads were generated from key zebrafish tissues  including the brain  testis  liver  eye  muscle  ovary  inner ear and kidney  to identify novel isoforms  tissue specific transcripts  and alternative splicing events. We also made available the Iso Seq data from embryos from different time points  hpf  0  6  12  and 24. This dataset enhances gene annotation  improves reference genome annotations  and provides insights into zebrafish organ specific gene expression.", null, null, null, "Iso Seq RNA from embryo zero hpf", "C2 F2 0", null, "strain:NHGRI2|isolate:Single paired cross|breed:Zebrafish|cultivar:wild type|ecotype:United States|age:time 0 hours|dev stage:Embyoys|collection date:2024 06|geo loc name:USA|sex:female|tissue:muscle|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Iso Seq RNA from embyo 0 hpf", "C2 F2 0", "C2 F2 0", "The long Read Sequencing libraries was sequenced using PacBio Sequel II", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "SINGLE", "PACBIO_SMRT", "Sequel II", null, "SRP568323", null, null, "m54313U_231110_180503.hifi_reads.flnc.fastq.gz", "fastq", 8144050440.0, 3744842.0, "m54313U 231110 180503.hifi reads.flnc.fastq.gz", "0:2174.74", "A:2267401658;C:1836982566;G:1935470734;T:2104195482;N:0", 2174, null, null, null, 2267401658, 1836982566, 1935470734, 2104195482, 0, "SRX27895216", "SRS24266218", "SRA2089085", "National Human Genome Research Institute|Translational and Functional Genomics Branch", "National Human Genome Research Institute National Human Genome Research Institute", 1, 0.76793, null, 0.00473, null, 0.80371, null, 0.50925, null, 1489, null, "T", null, "long read", "pacbio", "pacbio_modern", "unknown", "cdna_unspecified", "unknown", "bulk", "unknown", "unknown", null, "United States", "2025-03-06", "Zygote", "Embryo", "Muscle", "Muscular System"], [50511, "SRR8293425", "SRX5108028", "SRS4117494", "SRP172911", "PRJNA498365", "Raw sequence reads  in the unfertilized eggs of zebrafish Danio rerio", "PRJNA498365", "Other", "Raw sequence reads of full length transcriptome sequencing in the unfertilized eggs of zebrafish Danio rerio.", null, null, "Three female zebrafish were selected and 100 unfertilized eggs from each female were harvested as three samples Z1 1  Z1 2  and Z1 3 respectively; Identical quantities of RNA from Z1 1  Z1 2  and Z1 3 were combined into sample Z1 M.", "Model organism or animal sample from Danio rerio", "unfertilized eggs from three female zebrafish were harvested as samples Z1 M", null, "isolate:3 female zebrafish were selected and 100 unfertilized eggs from each female were harvested as 3 samples Z1 1  Z1 2  and Z1 3 respectively; and identical quantities of RNA from Z1 1  Z1 2  and Z1 3 were combined into sample Z1 M.|dev stage:unfertilized egg|sex:female|tissue:ovary|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Full length transcriptome sequencing of zebrafish Danio rerio : adult female unfertilized eggs.", "2 3K D03 1", "2 3K D03 1", "RNA sample Z1 M was reverse transcribed into cDNA. Four fragment ranges 0.5 1K  1 2K  2 3K  and >3K were prepared according to the PacBio Iso Seq protocol. PacBio RS II sequencing reactions of 16 SMRT single molecule real time cells 3 cells of 0.5 1K  5 cells of 1 2K  5 cells of 2 3K  3 cells of >3K were performed in DNA Sequencing Reagent 4.0 Clontech  Mountain View  California  USA.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "SINGLE", "PACBIO_SMRT", "PacBio RS II", null, "SRP172911", null, null, "m160828_125709_42199_c101055502550000001823247601061763_s1_p0.1.bax.h5 m160828_125709_42199_c101055502550000001823247601061763_s1_p0.2.bax.h5 m160828_125709_42199_c101055502550000001823247601061763_s1_p0.3.bax.h5 m160828_125709_42199_c101055502550000001823247601061763_s1_p0.bas.h5", "pacbio_native pacbio_native pacbio_native pacbio_native", 3189114337.0, 163482.0, "2 3K D03 1.tar.gz", null, "A:1019691460;C:775938787;G:628460211;T:736274173;N:0", null, null, null, null, 1019691460, 775938787, 628460211, 736274173, 0, "SRX5108028", "SRS4117494", "SRA820074", "Institute of Hydrobiology, Chinese Academy of Sciences|State Key Laboratory of Freshwater Ecology and Bio", "Institute of Hydrobiology, Chinese Academy of Sciences", null, null, null, null, null, null, null, null, null, null, null, null, null, null, "pacbio", "pacbio_early", "full_length", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2018-12-10", "Zygote", "Embryo", "Gonad", "Reproductive System"], [50512, "SRR8293426", "SRX5108027", "SRS4117494", "SRP172911", "PRJNA498365", "Raw sequence reads  in the unfertilized eggs of zebrafish Danio rerio", "PRJNA498365", "Other", "Raw sequence reads of full length transcriptome sequencing in the unfertilized eggs of zebrafish Danio rerio.", null, null, "Three female zebrafish were selected and 100 unfertilized eggs from each female were harvested as three samples Z1 1  Z1 2  and Z1 3 respectively; Identical quantities of RNA from Z1 1  Z1 2  and Z1 3 were combined into sample Z1 M.", "Model organism or animal sample from Danio rerio", "unfertilized eggs from three female zebrafish were harvested as samples Z1 M", null, "isolate:3 female zebrafish were selected and 100 unfertilized eggs from each female were harvested as 3 samples Z1 1  Z1 2  and Z1 3 respectively; and identical quantities of RNA from Z1 1  Z1 2  and Z1 3 were combined into sample Z1 M.|dev stage:unfertilized egg|sex:female|tissue:ovary|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Full length transcriptome sequencing of zebrafish Danio rerio : adult female unfertilized eggs.", "2 3K E03 1", "2 3K E03 1", "RNA sample Z1 M was reverse transcribed into cDNA. Four fragment ranges 0.5 1K  1 2K  2 3K  and >3K were prepared according to the PacBio Iso Seq protocol. PacBio RS II sequencing reactions of 16 SMRT single molecule real time cells 3 cells of 0.5 1K  5 cells of 1 2K  5 cells of 2 3K  3 cells of >3K were performed in DNA Sequencing Reagent 4.0 Clontech  Mountain View  California  USA.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "SINGLE", "PACBIO_SMRT", "PacBio RS II", null, "SRP172911", null, null, "m160828_171546_42199_c101055502550000001823247601061764_s1_p0.1.bax.h5 m160828_171546_42199_c101055502550000001823247601061764_s1_p0.2.bax.h5 m160828_171546_42199_c101055502550000001823247601061764_s1_p0.3.bax.h5 m160828_171546_42199_c101055502550000001823247601061764_s1_p0.bas.h5", "pacbio_native pacbio_native pacbio_native pacbio_native", 3603145484.0, 163482.0, "2 3K E03 1.tar.gz", null, "A:1041332538;C:789941375;G:785608794;T:952634306;N:0", null, null, null, null, 1041332538, 789941375, 785608794, 952634306, 0, "SRX5108027", "SRS4117494", "SRA820074", "Institute of Hydrobiology, Chinese Academy of Sciences|State Key Laboratory of Freshwater Ecology and Bio", "Institute of Hydrobiology, Chinese Academy of Sciences", null, null, null, null, null, null, null, null, null, null, null, null, null, null, "pacbio", "pacbio_early", "full_length", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2018-12-10", "Zygote", "Embryo", "Gonad", "Reproductive System"], [50513, "SRR8293427", "SRX5108026", "SRS4117494", "SRP172911", "PRJNA498365", "Raw sequence reads  in the unfertilized eggs of zebrafish Danio rerio", "PRJNA498365", "Other", "Raw sequence reads of full length transcriptome sequencing in the unfertilized eggs of zebrafish Danio rerio.", null, null, "Three female zebrafish were selected and 100 unfertilized eggs from each female were harvested as three samples Z1 1  Z1 2  and Z1 3 respectively; Identical quantities of RNA from Z1 1  Z1 2  and Z1 3 were combined into sample Z1 M.", "Model organism or animal sample from Danio rerio", "unfertilized eggs from three female zebrafish were harvested as samples Z1 M", null, "isolate:3 female zebrafish were selected and 100 unfertilized eggs from each female were harvested as 3 samples Z1 1  Z1 2  and Z1 3 respectively; and identical quantities of RNA from Z1 1  Z1 2  and Z1 3 were combined into sample Z1 M.|dev stage:unfertilized egg|sex:female|tissue:ovary|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Full length transcriptome sequencing of zebrafish Danio rerio : adult female unfertilized eggs.", "1 2K D05 1", "1 2K D05 1", "RNA sample Z1 M was reverse transcribed into cDNA. Four fragment ranges 0.5 1K  1 2K  2 3K  and >3K were prepared according to the PacBio Iso Seq protocol. PacBio RS II sequencing reactions of 16 SMRT single molecule real time cells 3 cells of 0.5 1K  5 cells of 1 2K  5 cells of 2 3K  3 cells of >3K were performed in DNA Sequencing Reagent 4.0 Clontech  Mountain View  California  USA.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "SINGLE", "PACBIO_SMRT", "PacBio RS II", null, "SRP172911", null, null, "m160831_064528_42199_c101016682550000001823228410211623_s1_p0.1.bax.h5 m160831_064528_42199_c101016682550000001823228410211623_s1_p0.2.bax.h5 m160831_064528_42199_c101016682550000001823228410211623_s1_p0.3.bax.h5 m160831_064528_42199_c101016682550000001823228410211623_s1_p0.bas.h5", "pacbio_native pacbio_native pacbio_native pacbio_native", 4494598972.0, 163482.0, "1 2K D05 1.tar.gz", null, "A:1263523570;C:919488657;G:1005901638;T:1241749988;N:0", null, null, null, null, 1263523570, 919488657, 1005901638, 1241749988, 0, "SRX5108026", "SRS4117494", "SRA820074", "Institute of Hydrobiology, Chinese Academy of Sciences|State Key Laboratory of Freshwater Ecology and Bio", "Institute of Hydrobiology, Chinese Academy of Sciences", null, null, null, null, null, null, null, null, null, null, null, null, null, null, "pacbio", "pacbio_early", "full_length", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2018-12-10", "Zygote", "Embryo", "Gonad", "Reproductive System"], [50514, "SRR8293428", "SRX5108025", "SRS4117494", "SRP172911", "PRJNA498365", "Raw sequence reads  in the unfertilized eggs of zebrafish Danio rerio", "PRJNA498365", "Other", "Raw sequence reads of full length transcriptome sequencing in the unfertilized eggs of zebrafish Danio rerio.", null, null, "Three female zebrafish were selected and 100 unfertilized eggs from each female were harvested as three samples Z1 1  Z1 2  and Z1 3 respectively; Identical quantities of RNA from Z1 1  Z1 2  and Z1 3 were combined into sample Z1 M.", "Model organism or animal sample from Danio rerio", "unfertilized eggs from three female zebrafish were harvested as samples Z1 M", null, "isolate:3 female zebrafish were selected and 100 unfertilized eggs from each female were harvested as 3 samples Z1 1  Z1 2  and Z1 3 respectively; and identical quantities of RNA from Z1 1  Z1 2  and Z1 3 were combined into sample Z1 M.|dev stage:unfertilized egg|sex:female|tissue:ovary|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Full length transcriptome sequencing of zebrafish Danio rerio : adult female unfertilized eggs.", "1 2K E05 1", "1 2K E05 1", "RNA sample Z1 M was reverse transcribed into cDNA. Four fragment ranges 0.5 1K  1 2K  2 3K  and >3K were prepared according to the PacBio Iso Seq protocol. PacBio RS II sequencing reactions of 16 SMRT single molecule real time cells 3 cells of 0.5 1K  5 cells of 1 2K  5 cells of 2 3K  3 cells of >3K were performed in DNA Sequencing Reagent 4.0 Clontech  Mountain View  California  USA.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "SINGLE", "PACBIO_SMRT", "PacBio RS II", null, "SRP172911", null, null, "m160831_110725_42199_c101016682550000001823228410211624_s1_p0.1.bax.h5 m160831_110725_42199_c101016682550000001823228410211624_s1_p0.2.bax.h5 m160831_110725_42199_c101016682550000001823228410211624_s1_p0.3.bax.h5 m160831_110725_42199_c101016682550000001823228410211624_s1_p0.bas.h5", "pacbio_native pacbio_native pacbio_native pacbio_native", 4610281055.0, 163482.0, "1 2K E05 1.tar.gz", null, "A:1313884618;C:971754386;G:994748647;T:1264950574;N:0", null, null, null, null, 1313884618, 971754386, 994748647, 1264950574, 0, "SRX5108025", "SRS4117494", "SRA820074", "Institute of Hydrobiology, Chinese Academy of Sciences|State Key Laboratory of Freshwater Ecology and Bio", "Institute of Hydrobiology, Chinese Academy of Sciences", null, null, null, null, null, null, null, null, null, null, null, null, null, null, "pacbio", "pacbio_early", "full_length", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2018-12-10", "Zygote", "Embryo", "Gonad", "Reproductive System"], [50515, "SRR8293429", "SRX5108024", "SRS4117494", "SRP172911", "PRJNA498365", "Raw sequence reads  in the unfertilized eggs of zebrafish Danio rerio", "PRJNA498365", "Other", "Raw sequence reads of full length transcriptome sequencing in the unfertilized eggs of zebrafish Danio rerio.", null, null, "Three female zebrafish were selected and 100 unfertilized eggs from each female were harvested as three samples Z1 1  Z1 2  and Z1 3 respectively; Identical quantities of RNA from Z1 1  Z1 2  and Z1 3 were combined into sample Z1 M.", "Model organism or animal sample from Danio rerio", "unfertilized eggs from three female zebrafish were harvested as samples Z1 M", null, "isolate:3 female zebrafish were selected and 100 unfertilized eggs from each female were harvested as 3 samples Z1 1  Z1 2  and Z1 3 respectively; and identical quantities of RNA from Z1 1  Z1 2  and Z1 3 were combined into sample Z1 M.|dev stage:unfertilized egg|sex:female|tissue:ovary|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Full length transcriptome sequencing of zebrafish Danio rerio : adult female unfertilized eggs.", "1 2K F03 1", "1 2K F03 1", "RNA sample Z1 M was reverse transcribed into cDNA. Four fragment ranges 0.5 1K  1 2K  2 3K  and >3K were prepared according to the PacBio Iso Seq protocol. PacBio RS II sequencing reactions of 16 SMRT single molecule real time cells 3 cells of 0.5 1K  5 cells of 1 2K  5 cells of 2 3K  3 cells of >3K were performed in DNA Sequencing Reagent 4.0 Clontech  Mountain View  California  USA.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "SINGLE", "PACBIO_SMRT", "PacBio RS II", null, "SRP172911", null, null, "m160828_213458_42199_c101055502550000001823247601061765_s1_p0.1.bax.h5 m160828_213458_42199_c101055502550000001823247601061765_s1_p0.2.bax.h5 m160828_213458_42199_c101055502550000001823247601061765_s1_p0.3.bax.h5 m160828_213458_42199_c101055502550000001823247601061765_s1_p0.bas.h5", "pacbio_native pacbio_native pacbio_native pacbio_native", 6488051235.0, 163482.0, "1 2K F03 1.tar.gz", null, "A:1835535517;C:1366315971;G:1464006715;T:1752025312;N:0", null, null, null, null, 1835535517, 1366315971, 1464006715, 1752025312, 0, "SRX5108024", "SRS4117494", "SRA820074", "Institute of Hydrobiology, Chinese Academy of Sciences|State Key Laboratory of Freshwater Ecology and Bio", "Institute of Hydrobiology, Chinese Academy of Sciences", null, null, null, null, null, null, null, null, null, null, null, null, null, null, "pacbio", "pacbio_early", "full_length", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2018-12-10", "Zygote", "Embryo", "Gonad", "Reproductive System"], [50516, "SRR8293430", "SRX5108023", "SRS4117494", "SRP172911", "PRJNA498365", "Raw sequence reads  in the unfertilized eggs of zebrafish Danio rerio", "PRJNA498365", "Other", "Raw sequence reads of full length transcriptome sequencing in the unfertilized eggs of zebrafish Danio rerio.", null, null, "Three female zebrafish were selected and 100 unfertilized eggs from each female were harvested as three samples Z1 1  Z1 2  and Z1 3 respectively; Identical quantities of RNA from Z1 1  Z1 2  and Z1 3 were combined into sample Z1 M.", "Model organism or animal sample from Danio rerio", "unfertilized eggs from three female zebrafish were harvested as samples Z1 M", null, "isolate:3 female zebrafish were selected and 100 unfertilized eggs from each female were harvested as 3 samples Z1 1  Z1 2  and Z1 3 respectively; and identical quantities of RNA from Z1 1  Z1 2  and Z1 3 were combined into sample Z1 M.|dev stage:unfertilized egg|sex:female|tissue:ovary|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Full length transcriptome sequencing of zebrafish Danio rerio : adult female unfertilized eggs.", "1 2K G05 1", "1 2K G05 1", "RNA sample Z1 M was reverse transcribed into cDNA. Four fragment ranges 0.5 1K  1 2K  2 3K  and >3K were prepared according to the PacBio Iso Seq protocol. 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Four fragment ranges 0.5 1K  1 2K  2 3K  and >3K were prepared according to the PacBio Iso Seq protocol. PacBio RS II sequencing reactions of 16 SMRT single molecule real time cells 3 cells of 0.5 1K  5 cells of 1 2K  5 cells of 2 3K  3 cells of >3K were performed in DNA Sequencing Reagent 4.0 Clontech  Mountain View  California  USA.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "SINGLE", "PACBIO_SMRT", "PacBio RS II", null, "SRP172911", null, null, "m160921_142928_42199_c100979762550000001823212907061657_s1_X0.bas.h5 m160921_142928_42199_c100979762550000001823212907061657_s1_X0.3.bax.h5 m160921_142928_42199_c100979762550000001823212907061657_s1_X0.2.bax.h5 m160921_142928_42199_c100979762550000001823212907061657_s1_X0.1.bax.h5", "pacbio_native pacbio_native pacbio_native pacbio_native", 1906153604.0, 163482.0, "0.5 1K E03 1.tar.gz", null, "A:568523342;C:407560314;G:389613623;T:500427928;N:0", null, null, null, null, 568523342, 407560314, 389613623, 500427928, 0, "SRX5108022", "SRS4117494", "SRA820074", "Institute of Hydrobiology, Chinese Academy of Sciences|State Key Laboratory of Freshwater Ecology and Bio", "Institute of Hydrobiology, Chinese Academy of Sciences", null, null, null, null, null, null, null, null, null, null, null, null, null, null, "pacbio", "pacbio_early", "full_length", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2018-12-08", "Zygote", "Embryo", "Gonad", "Reproductive System"], [50518, "SRR8293432", "SRX5108021", "SRS4117494", "SRP172911", "PRJNA498365", "Raw sequence reads  in the unfertilized eggs of zebrafish Danio rerio", "PRJNA498365", "Other", "Raw sequence reads of full length transcriptome sequencing in the unfertilized eggs of zebrafish Danio rerio.", null, null, "Three female zebrafish were selected and 100 unfertilized eggs from each female were harvested as three samples Z1 1  Z1 2  and Z1 3 respectively; Identical quantities of RNA from Z1 1  Z1 2  and Z1 3 were combined into sample Z1 M.", "Model organism or animal sample from Danio rerio", "unfertilized eggs from three female zebrafish were harvested as samples Z1 M", null, "isolate:3 female zebrafish were selected and 100 unfertilized eggs from each female were harvested as 3 samples Z1 1  Z1 2  and Z1 3 respectively; and identical quantities of RNA from Z1 1  Z1 2  and Z1 3 were combined into sample Z1 M.|dev stage:unfertilized egg|sex:female|tissue:ovary|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Full length transcriptome sequencing of zebrafish Danio rerio : adult female unfertilized eggs.", "0.5 1K F03 1", "0.5 1K F03 1", "RNA sample Z1 M was reverse transcribed into cDNA. Four fragment ranges 0.5 1K  1 2K  2 3K  and >3K were prepared according to the PacBio Iso Seq protocol. PacBio RS II sequencing reactions of 16 SMRT single molecule real time cells 3 cells of 0.5 1K  5 cells of 1 2K  5 cells of 2 3K  3 cells of >3K were performed in DNA Sequencing Reagent 4.0 Clontech  Mountain View  California  USA.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "SINGLE", "PACBIO_SMRT", "PacBio RS II", null, "SRP172911", null, null, "m160921_184841_42199_c101055652550000001823238312291647_s1_p0.1.bax.h5 m160921_184841_42199_c101055652550000001823238312291647_s1_p0.2.bax.h5 m160921_184841_42199_c101055652550000001823238312291647_s1_p0.3.bax.h5 m160921_184841_42199_c101055652550000001823238312291647_s1_p0.bas.h5", "pacbio_native pacbio_native pacbio_native pacbio_native", 4156910909.0, 163482.0, "0.5 1K F03 1.tar.gz", null, "A:1183616929;C:878682771;G:890537851;T:1136413189;N:0", null, null, null, null, 1183616929, 878682771, 890537851, 1136413189, 0, "SRX5108021", "SRS4117494", "SRA820074", "Institute of Hydrobiology, Chinese Academy of Sciences|State Key Laboratory of Freshwater Ecology and Bio", "Institute of Hydrobiology, Chinese Academy of Sciences", null, null, null, null, null, null, null, null, null, null, null, null, null, null, "pacbio", "pacbio_early", "full_length", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2018-12-10", "Zygote", "Embryo", "Gonad", "Reproductive System"], [50519, "SRR8293433", "SRX5108020", "SRS4117494", "SRP172911", "PRJNA498365", "Raw sequence reads  in the unfertilized eggs of zebrafish Danio rerio", "PRJNA498365", "Other", "Raw sequence reads of full length transcriptome sequencing in the unfertilized eggs of zebrafish Danio rerio.", null, null, "Three female zebrafish were selected and 100 unfertilized eggs from each female were harvested as three samples Z1 1  Z1 2  and Z1 3 respectively; Identical quantities of RNA from Z1 1  Z1 2  and Z1 3 were combined into sample Z1 M.", "Model organism or animal sample from Danio rerio", "unfertilized eggs from three female zebrafish were harvested as samples Z1 M", null, "isolate:3 female zebrafish were selected and 100 unfertilized eggs from each female were harvested as 3 samples Z1 1  Z1 2  and Z1 3 respectively; and identical quantities of RNA from Z1 1  Z1 2  and Z1 3 were combined into sample Z1 M.|dev stage:unfertilized egg|sex:female|tissue:ovary|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Full length transcriptome sequencing of zebrafish Danio rerio : adult female unfertilized eggs.", "0.5 1K G03 1", "0.5 1K G03 1", "RNA sample Z1 M was reverse transcribed into cDNA. Four fragment ranges 0.5 1K  1 2K  2 3K  and >3K were prepared according to the PacBio Iso Seq protocol. PacBio RS II sequencing reactions of 16 SMRT single molecule real time cells 3 cells of 0.5 1K  5 cells of 1 2K  5 cells of 2 3K  3 cells of >3K were performed in DNA Sequencing Reagent 4.0 Clontech  Mountain View  California  USA.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "SINGLE", "PACBIO_SMRT", "PacBio RS II", null, "SRP172911", null, null, "m160829_063211_42199_c101055502550000001823247601061767_s1_p0.bas.h5 m160829_063211_42199_c101055502550000001823247601061767_s1_p0.3.bax.h5 m160829_063211_42199_c101055502550000001823247601061767_s1_p0.2.bax.h5 m160829_063211_42199_c101055502550000001823247601061767_s1_p0.1.bax.h5", "pacbio_native pacbio_native pacbio_native pacbio_native", 5660530911.0, 163482.0, "0.5 1K H03 1.tar.gz", null, "A:1835439052;C:1406954743;G:1037776936;T:1307349158;N:0", null, null, null, null, 1835439052, 1406954743, 1037776936, 1307349158, 0, "SRX5108020", "SRS4117494", "SRA820074", "Institute of Hydrobiology, Chinese Academy of Sciences|State Key Laboratory of Freshwater Ecology and Bio", "Institute of Hydrobiology, Chinese Academy of Sciences", null, null, null, null, null, null, null, null, null, null, null, null, null, null, "pacbio", "pacbio_early", "full_length", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2018-12-08", "Zygote", "Embryo", "Gonad", "Reproductive System"], [50520, "SRR8293434", "SRX5108019", "SRS4117494", "SRP172911", "PRJNA498365", "Raw sequence reads  in the unfertilized eggs of zebrafish Danio rerio", "PRJNA498365", "Other", "Raw sequence reads of full length transcriptome sequencing in the unfertilized eggs of zebrafish Danio rerio.", null, null, "Three female zebrafish were selected and 100 unfertilized eggs from each female were harvested as three samples Z1 1  Z1 2  and Z1 3 respectively; Identical quantities of RNA from Z1 1  Z1 2  and Z1 3 were combined into sample Z1 M.", "Model organism or animal sample from Danio rerio", "unfertilized eggs from three female zebrafish were harvested as samples Z1 M", null, "isolate:3 female zebrafish were selected and 100 unfertilized eggs from each female were harvested as 3 samples Z1 1  Z1 2  and Z1 3 respectively; and identical quantities of RNA from Z1 1  Z1 2  and Z1 3 were combined into sample Z1 M.|dev stage:unfertilized egg|sex:female|tissue:ovary|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Full length transcriptome sequencing of zebrafish Danio rerio : adult female unfertilized eggs.", "1 2K C05 1", "1 2K C05 1", "RNA sample Z1 M was reverse transcribed into cDNA. Four fragment ranges 0.5 1K  1 2K  2 3K  and >3K were prepared according to the PacBio Iso Seq protocol. PacBio RS II sequencing reactions of 16 SMRT single molecule real time cells 3 cells of 0.5 1K  5 cells of 1 2K  5 cells of 2 3K  3 cells of >3K were performed in DNA Sequencing Reagent 4.0 Clontech  Mountain View  California  USA.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "SINGLE", "PACBIO_SMRT", "PacBio RS II", null, "SRP172911", null, null, "m160831_022615_42199_c101016682550000001823228410211622_s1_p0.1.bax.h5 m160831_022615_42199_c101016682550000001823228410211622_s1_p0.2.bax.h5 m160831_022615_42199_c101016682550000001823228410211622_s1_p0.3.bax.h5 m160831_022615_42199_c101016682550000001823228410211622_s1_p0.bas.h5", "pacbio_native pacbio_native pacbio_native pacbio_native", 3047073997.0, 163482.0, "1 2K C05 1.tar.gz", null, "A:862003813;C:632482019;G:660595007;T:843966997;N:0", null, null, null, null, 862003813, 632482019, 660595007, 843966997, 0, "SRX5108019", "SRS4117494", "SRA820074", "Institute of Hydrobiology, Chinese Academy of Sciences|State Key Laboratory of Freshwater Ecology and Bio", "Institute of Hydrobiology, Chinese Academy of Sciences", null, null, null, null, null, null, null, null, null, null, null, null, null, null, "pacbio", "pacbio_early", "full_length", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2018-12-10", "Zygote", "Embryo", "Gonad", "Reproductive System"], [50521, "SRR8293435", "SRX5108018", "SRS4117494", "SRP172911", "PRJNA498365", "Raw sequence reads  in the unfertilized eggs of zebrafish Danio rerio", "PRJNA498365", "Other", "Raw sequence reads of full length transcriptome sequencing in the unfertilized eggs of zebrafish Danio rerio.", null, null, "Three female zebrafish were selected and 100 unfertilized eggs from each female were harvested as three samples Z1 1  Z1 2  and Z1 3 respectively; Identical quantities of RNA from Z1 1  Z1 2  and Z1 3 were combined into sample Z1 M.", "Model organism or animal sample from Danio rerio", "unfertilized eggs from three female zebrafish were harvested as samples Z1 M", null, "isolate:3 female zebrafish were selected and 100 unfertilized eggs from each female were harvested as 3 samples Z1 1  Z1 2  and Z1 3 respectively; and identical quantities of RNA from Z1 1  Z1 2  and Z1 3 were combined into sample Z1 M.|dev stage:unfertilized egg|sex:female|tissue:ovary|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Full length transcriptome sequencing of zebrafish Danio rerio : adult female unfertilized eggs.", "2 3K F05 1", "2 3K F05 1", "RNA sample Z1 M was reverse transcribed into cDNA. Four fragment ranges 0.5 1K  1 2K  2 3K  and >3K were prepared according to the PacBio Iso Seq protocol. PacBio RS II sequencing reactions of 16 SMRT single molecule real time cells 3 cells of 0.5 1K  5 cells of 1 2K  5 cells of 2 3K  3 cells of >3K were performed in DNA Sequencing Reagent 4.0 Clontech  Mountain View  California  USA.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "SINGLE", "PACBIO_SMRT", "PacBio RS II", null, "SRP172911", null, null, "m160831_152721_42199_c101016682550000001823228410211625_s1_p0.1.bax.h5 m160831_152721_42199_c101016682550000001823228410211625_s1_p0.2.bax.h5 m160831_152721_42199_c101016682550000001823228410211625_s1_p0.3.bax.h5 m160831_152721_42199_c101016682550000001823228410211625_s1_p0.bas.h5", "pacbio_native pacbio_native pacbio_native pacbio_native", 3504827488.0, 163482.0, "2 3K F05 1.tar.gz", null, "A:1035191904;C:787763869;G:735456262;T:916015813;N:0", null, null, null, null, 1035191904, 787763869, 735456262, 916015813, 0, "SRX5108018", "SRS4117494", "SRA820074", "Institute of Hydrobiology, Chinese Academy of Sciences|State Key Laboratory of Freshwater Ecology and Bio", "Institute of Hydrobiology, Chinese Academy of Sciences", null, null, null, null, null, null, null, null, null, null, null, null, null, null, "pacbio", "pacbio_early", "full_length", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2018-12-10", "Zygote", "Embryo", "Gonad", "Reproductive System"], [50522, "SRR8293436", "SRX5108017", "SRS4117494", "SRP172911", "PRJNA498365", "Raw sequence reads  in the unfertilized eggs of zebrafish Danio rerio", "PRJNA498365", "Other", "Raw sequence reads of full length transcriptome sequencing in the unfertilized eggs of zebrafish Danio rerio.", null, null, "Three female zebrafish were selected and 100 unfertilized eggs from each female were harvested as three samples Z1 1  Z1 2  and Z1 3 respectively; Identical quantities of RNA from Z1 1  Z1 2  and Z1 3 were combined into sample Z1 M.", "Model organism or animal sample from Danio rerio", "unfertilized eggs from three female zebrafish were harvested as samples Z1 M", null, "isolate:3 female zebrafish were selected and 100 unfertilized eggs from each female were harvested as 3 samples Z1 1  Z1 2  and Z1 3 respectively; and identical quantities of RNA from Z1 1  Z1 2  and Z1 3 were combined into sample Z1 M.|dev stage:unfertilized egg|sex:female|tissue:ovary|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Full length transcriptome sequencing of zebrafish Danio rerio : adult female unfertilized eggs.", "2 3K G05 1", "2 3K G05 1", "RNA sample Z1 M was reverse transcribed into cDNA. Four fragment ranges 0.5 1K  1 2K  2 3K  and >3K were prepared according to the PacBio Iso Seq protocol. PacBio RS II sequencing reactions of 16 SMRT single molecule real time cells 3 cells of 0.5 1K  5 cells of 1 2K  5 cells of 2 3K  3 cells of >3K were performed in DNA Sequencing Reagent 4.0 Clontech  Mountain View  California  USA.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "SINGLE", "PACBIO_SMRT", "PacBio RS II", null, "SRP172911", null, null, "m160831_195005_42199_c101016682550000001823228410211626_s1_p0.1.bax.h5 m160831_195005_42199_c101016682550000001823228410211626_s1_p0.2.bax.h5 m160831_195005_42199_c101016682550000001823228410211626_s1_p0.3.bax.h5 m160831_195005_42199_c101016682550000001823228410211626_s1_p0.bas.h5", "pacbio_native pacbio_native pacbio_native pacbio_native", 3325636539.0, 163482.0, "2 3K G05 1.tar.gz", null, "A:945624516;C:712334057;G:726829680;T:910995593;N:0", null, null, null, null, 945624516, 712334057, 726829680, 910995593, 0, "SRX5108017", "SRS4117494", "SRA820074", "Institute of Hydrobiology, Chinese Academy of Sciences|State Key Laboratory of Freshwater Ecology and Bio", "Institute of Hydrobiology, Chinese Academy of Sciences", null, null, null, null, null, null, null, null, null, null, null, null, null, null, "pacbio", "pacbio_early", "full_length", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2018-12-10", "Zygote", "Embryo", "Gonad", "Reproductive System"], [50523, "SRR8293437", "SRX5108016", "SRS4117494", "SRP172911", "PRJNA498365", "Raw sequence reads  in the unfertilized eggs of zebrafish Danio rerio", "PRJNA498365", "Other", "Raw sequence reads of full length transcriptome sequencing in the unfertilized eggs of zebrafish Danio rerio.", null, null, "Three female zebrafish were selected and 100 unfertilized eggs from each female were harvested as three samples Z1 1  Z1 2  and Z1 3 respectively; Identical quantities of RNA from Z1 1  Z1 2  and Z1 3 were combined into sample Z1 M.", "Model organism or animal sample from Danio rerio", "unfertilized eggs from three female zebrafish were harvested as samples Z1 M", null, "isolate:3 female zebrafish were selected and 100 unfertilized eggs from each female were harvested as 3 samples Z1 1  Z1 2  and Z1 3 respectively; and identical quantities of RNA from Z1 1  Z1 2  and Z1 3 were combined into sample Z1 M.|dev stage:unfertilized egg|sex:female|tissue:ovary|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Full length transcriptome sequencing of zebrafish Danio rerio : adult female unfertilized eggs.", "2 3K H05 1", "2 3K H05 1", "RNA sample Z1 M was reverse transcribed into cDNA. Four fragment ranges 0.5 1K  1 2K  2 3K  and >3K were prepared according to the PacBio Iso Seq protocol. PacBio RS II sequencing reactions of 16 SMRT single molecule real time cells 3 cells of 0.5 1K  5 cells of 1 2K  5 cells of 2 3K  3 cells of >3K were performed in DNA Sequencing Reagent 4.0 Clontech  Mountain View  California  USA.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "SINGLE", "PACBIO_SMRT", "PacBio RS II", null, "SRP172911", null, null, "m160901_000924_42199_c101016682550000001823228410211627_s1_p0.1.bax.h5 m160901_000924_42199_c101016682550000001823228410211627_s1_p0.2.bax.h5 m160901_000924_42199_c101016682550000001823228410211627_s1_p0.3.bax.h5 m160901_000924_42199_c101016682550000001823228410211627_s1_p0.bas.h5", "pacbio_native pacbio_native pacbio_native pacbio_native", 3219471171.0, 163482.0, "2 3K H05 1.tar.gz", null, "A:955121582;C:717645710;G:671893655;T:846270302;N:0", null, null, null, null, 955121582, 717645710, 671893655, 846270302, 0, "SRX5108016", "SRS4117494", "SRA820074", "Institute of Hydrobiology, Chinese Academy of Sciences|State Key Laboratory of Freshwater Ecology and Bio", "Institute of Hydrobiology, Chinese Academy of Sciences", null, null, null, null, null, null, null, null, null, null, null, null, null, null, "pacbio", "pacbio_early", "full_length", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2018-12-10", "Zygote", "Embryo", "Gonad", "Reproductive System"], [50524, "SRR8293438", "SRX5108015", "SRS4117494", "SRP172911", "PRJNA498365", "Raw sequence reads  in the unfertilized eggs of zebrafish Danio rerio", "PRJNA498365", "Other", "Raw sequence reads of full length transcriptome sequencing in the unfertilized eggs of zebrafish Danio rerio.", null, null, "Three female zebrafish were selected and 100 unfertilized eggs from each female were harvested as three samples Z1 1  Z1 2  and Z1 3 respectively; Identical quantities of RNA from Z1 1  Z1 2  and Z1 3 were combined into sample Z1 M.", "Model organism or animal sample from Danio rerio", "unfertilized eggs from three female zebrafish were harvested as samples Z1 M", null, "isolate:3 female zebrafish were selected and 100 unfertilized eggs from each female were harvested as 3 samples Z1 1  Z1 2  and Z1 3 respectively; and identical quantities of RNA from Z1 1  Z1 2  and Z1 3 were combined into sample Z1 M.|dev stage:unfertilized egg|sex:female|tissue:ovary|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Full length transcriptome sequencing of zebrafish Danio rerio : adult female unfertilized eggs.", "3K plus C03 1", "3K plus C03 1", "RNA sample Z1 M was reverse transcribed into cDNA. Four fragment ranges 0.5 1K  1 2K  2 3K  and >3K were prepared according to the PacBio Iso Seq protocol. PacBio RS II sequencing reactions of 16 SMRT single molecule real time cells 3 cells of 0.5 1K  5 cells of 1 2K  5 cells of 2 3K  3 cells of >3K were performed in DNA Sequencing Reagent 4.0 Clontech  Mountain View  California  USA.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "SINGLE", "PACBIO_SMRT", "PacBio RS II", null, "SRP172911", null, null, "m160828_083719_42199_c101055502550000001823247601061762_s1_p0.1.bax.h5 m160828_083719_42199_c101055502550000001823247601061762_s1_p0.2.bax.h5 m160828_083719_42199_c101055502550000001823247601061762_s1_p0.3.bax.h5 m160828_083719_42199_c101055502550000001823247601061762_s1_p0.bas.h5", "pacbio_native pacbio_native pacbio_native pacbio_native", 2241335194.0, 163482.0, "3K plus C03 1.tar.gz", null, "A:714450646;C:554512788;G:442348736;T:513624736;N:0", null, null, null, null, 714450646, 554512788, 442348736, 513624736, 0, "SRX5108015", "SRS4117494", "SRA820074", "Institute of Hydrobiology, Chinese Academy of Sciences|State Key Laboratory of Freshwater Ecology and Bio", "Institute of Hydrobiology, Chinese Academy of Sciences", null, null, null, null, null, null, null, null, null, null, null, null, null, null, "pacbio", "pacbio_early", "full_length", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2018-12-10", "Zygote", "Embryo", "Gonad", "Reproductive System"], [50525, "SRR8293439", "SRX5108014", "SRS4117494", "SRP172911", "PRJNA498365", "Raw sequence reads  in the unfertilized eggs of zebrafish Danio rerio", "PRJNA498365", "Other", "Raw sequence reads of full length transcriptome sequencing in the unfertilized eggs of zebrafish Danio rerio.", null, null, "Three female zebrafish were selected and 100 unfertilized eggs from each female were harvested as three samples Z1 1  Z1 2  and Z1 3 respectively; Identical quantities of RNA from Z1 1  Z1 2  and Z1 3 were combined into sample Z1 M.", "Model organism or animal sample from Danio rerio", "unfertilized eggs from three female zebrafish were harvested as samples Z1 M", null, "isolate:3 female zebrafish were selected and 100 unfertilized eggs from each female were harvested as 3 samples Z1 1  Z1 2  and Z1 3 respectively; and identical quantities of RNA from Z1 1  Z1 2  and Z1 3 were combined into sample Z1 M.|dev stage:unfertilized egg|sex:female|tissue:ovary|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Full length transcriptome sequencing of zebrafish Danio rerio : adult female unfertilized eggs.", "3K plus G03 1", "3K plus G03 1", "RNA sample Z1 M was reverse transcribed into cDNA. Four fragment ranges 0.5 1K  1 2K  2 3K  and >3K were prepared according to the PacBio Iso Seq protocol. PacBio RS II sequencing reactions of 16 SMRT single molecule real time cells 3 cells of 0.5 1K  5 cells of 1 2K  5 cells of 2 3K  3 cells of >3K were performed in DNA Sequencing Reagent 4.0 Clontech  Mountain View  California  USA.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "RANDOM PCR", "SINGLE", "PACBIO_SMRT", "PacBio RS II", null, "SRP172911", null, null, "m160921_231100_42199_c100829932550000001823181912311557_s1_X0.1.bax.h5 m160921_231100_42199_c100829932550000001823181912311557_s1_X0.2.bax.h5 m160921_231100_42199_c100829932550000001823181912311557_s1_X0.3.bax.h5 m160921_231100_42199_c100829932550000001823181912311557_s1_X0.bas.h5", "pacbio_native pacbio_native pacbio_native pacbio_native", 1500621674.0, 163482.0, "3K plus G03 1.tar.gz", null, "A:461686513;C:353289117;G:298838025;T:376337789;N:0", null, null, null, null, 461686513, 353289117, 298838025, 376337789, 0, "SRX5108014", "SRS4117494", "SRA820074", "Institute of Hydrobiology, Chinese Academy of Sciences|State Key Laboratory of Freshwater Ecology and Bio", "Institute of Hydrobiology, Chinese Academy of Sciences", null, null, null, null, null, null, null, null, null, null, null, null, null, null, "pacbio", "pacbio_early", "full_length", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2018-12-10", "Zygote", "Embryo", "Gonad", "Reproductive System"], [50526, "SRR8293440", "SRX5108013", "SRS4117494", "SRP172911", "PRJNA498365", "Raw sequence reads  in the unfertilized eggs of zebrafish Danio rerio", "PRJNA498365", "Other", "Raw sequence reads of full length transcriptome sequencing in the unfertilized eggs of zebrafish Danio rerio.", null, null, "Three female zebrafish were selected and 100 unfertilized eggs from each female were harvested as three samples Z1 1  Z1 2  and Z1 3 respectively; Identical quantities of RNA from Z1 1  Z1 2  and Z1 3 were combined into sample Z1 M.", "Model organism or animal sample from Danio rerio", "unfertilized eggs from three female zebrafish were harvested as samples Z1 M", null, "isolate:3 female zebrafish were selected and 100 unfertilized eggs from each female were harvested as 3 samples Z1 1  Z1 2  and Z1 3 respectively; and identical quantities of RNA from Z1 1  Z1 2  and Z1 3 were combined into sample Z1 M.|dev stage:unfertilized egg|sex:female|tissue:ovary|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Full length transcriptome sequencing of zebrafish Danio rerio : adult female unfertilized eggs.", "3K plus H03 1", "3K plus H03 1", "RNA sample Z1 M was reverse transcribed into cDNA. 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Prime STAR GXL DNA Polymerase Kit was used to PCR amplify the full length cDNA. The SMARTbell libraries were constructed with the SMARTbell template prep kit. Conduct damage repair and end repair. 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