{"database": "metadata", "table": "run_metadata", "is_view": false, "human_description_en": "where devstage_curation = \"Gastrula\" and experiment.library_selection = \"RANDOM PCR\"", "rows": [[39629, "SRR1947863", "SRX981062", "SRS889614", "SRP056748", "PRJNA279991", "Zebrafish Mespaa regulates miR 430 expression during gastrulation and  initiates cardiac laterality", "PRJNA279991", "Other", "Transcriptional events during initial vertebrate heart development in vivo remain poorly understood. Mesp1  a bHLH transcription factor  has been described as the earliest transcriptional regulator of cardiac progenitors in multiple species  and represents an excellent candidate for the investigation of relevant targets during cardiovascular development. We report here that both depletion and mutation of Mespaa  the zebrafish homolog of mammalian Mesp1  lead to randomization of cardiac looping  together with significant cardiac morphogenesis defects. These disruptions are preceded by a defect in cardiac left right asymmetry. Surprisingly  the defect in asymmetry was found to occur independently of defects in the transient organ of laterality  the Kupffer\u2019s vesicle KV. We show that Mespaa regulates miR 430 expression during gastrulation to control the levels of Nodal signaling  and that this regulation is required for asymmetric laterality signaling in the prospective heart field. Ectopic expression of miR 430 is sufficient to induce cardiac laterality defects  and consistent with Mespaa over expression in this system  the reduction of miR 430 leads to cardia bifida. 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Ectopic expression of miR 430 is sufficient to induce cardiac laterality defects  and consistent with Mespaa over expression in this system  the reduction of miR 430 leads to cardia bifida. 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Ectopic expression of miR 430 is sufficient to induce cardiac laterality defects  and consistent with Mespaa over expression in this system  the reduction of miR 430 leads to cardia bifida. 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Ectopic expression of miR 430 is sufficient to induce cardiac laterality defects  and consistent with Mespaa over expression in this system  the reduction of miR 430 leads to cardia bifida. 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