{"database": "metadata", "table": "run_metadata", "is_view": false, "human_description_en": "where devstage_curation = \"Adult\" and tissue_curation_coarse = \"Skeletal Element\"", "rows": [[38060, "SRR1524244", "SRX661009", "SRS665984", "SRP044781", "PRJNA255848", "Danio rerio Transcriptome", "PRJNA255848", "Transcriptome Analysis", "Transcriptome analysis of 12 zebrafish tissues", "parent bioproject:PRJNA255979", "pubmed:27189481", "Zebrafish Bones", "Zebrafish B1s", "F Dr 8", null, "strain:AB|age:5 month|biomaterial provider:INRA|sex:female|tissue:B1s|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "Zebrafish B1s", "F Dr 8", "F Dr 8", "Total RNA was qualified using an Agilent BioAnalyzer and 1 \u00b5g was used for polyA selection and library construction with Illumina's TruSeq stranded total RNA sample preparation kit according to the manufacturer's instructions TruSeq stranded total RNA SamplePrep Guide RevC", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "Illumina HiSeq 2000", "<SPOT_DESCRIPTOR><SPOT_DECODE_SPEC><SPOT_LENGTH>200</SPOT_LENGTH><READ_SPEC><READ_INDEX>0</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Forward</READ_TYPE><BASE_COORD>1</BASE_COORD></READ_SPEC><READ_SPEC><READ_INDEX>1</READ_INDEX><READ_CLASS>Application Read</READ_CLASS><READ_TYPE>Reverse</READ_TYPE><BASE_COORD>101</BASE_COORD></READ_SPEC></SPOT_DECODE_SPEC></SPOT_DESCRIPTOR>", "SRP044781", null, null, "F_Dr_8_ACAGTG_L003_R2.fastq.gz F_Dr_8_ACAGTG_L003_R1.fastq.gz", "fastq fastq", 19343193000.0, 96715965.0, "F Dr 8 files", "0:100 1:100", "A:5011655819;C:4570121318;G:4634671917;T:4933523152;N:193220794", 100, 100, null, null, 5011655819, 4570121318, 4634671917, 4933523152, 193220794, "SRX661009", "SRS665984", "SRA176464", "INRA|Fish Physiology and Genomics", "INRA PhyloFish", 2, 0.88337, 0.91338, 0.05259, 0.05508, 0.75103, 0.75317, 0.51459, 0.51744, 100, 100, "B", "B", "biological fallback assumption", "illumina", "hiseq_era", "unknown", "poly_a", "trueseq", "bulk", "unknown", "unknown", null, "France", "2014-07-26", "Adult", "Adult", "Bone or Cartilage", "Skeletal Element"], [53148, "SRR9692022", "SRX6451056", "SRS5104327", "SRP214773", "PRJNA554788", "Gene expression analysis in the zebrafish fractured scale", "GSE134330", "Transcriptome Analysis", "Bone is a connective tissue which undergoes continuous remodeling  including bone resorption and formation by osteoclasts OCs and osteoblasts OBs  respectively. Recently  extracellular vesicles EVs are increasingly appreciated as a regulator of intercellular communication between OCs and OBs. The zebrafish scale is a thin membranous bone embedded in the skin and consists of OBs  OCs  and bone matrix  providing an elegant model to visualize OC OB communication. Here  we developed a double transgenic zebrafish line  trap:GFP; osterix:mCherry  which expresses GFP and mCherry under the control of the OC specific trap tartrate resistant acid phosphatase and OB specific osterix enhancer  respectively. Utilizing this double transgenic line  in combination with Hoechst 33342 Hoe staining  we isolated four distinct fractions from the scale at 1 day post fracture by flow cytometry  GFP\u2013 mCh+ Hoehigh OB fraction  GFPlow mCh+ Hoehigh OC precursor pOC fraction  GFPhigh Hoehigh mature OC mOC fraction  and mCh+ Hoe\u2013 OB derived extracellular vesicle EV fraction. In this study  we have performed RNA seq analysis using these four fractions to examine the gene expression profile of each  fraction. Overall design: mRNA profiles of isolated OBs  pOCs   mOCs  and OB derived EVs in the zebrafish fractured scales were generated by Quartz seq using Illumina NextSeq500.", null, "pubmed:32327701", null, "EV 2", "GSM3942348", null, "source name:osteoblast derived extracellular vesicles|age:adult|genotype:wild type|tissue:Sorted from the fractured sclae|cell type:osteoblast derived extracellular vesicles|molecule type:Amplified RNA", "EV 2", "Illumina RTA software was used for base calling. Sequence reads were mapped to the zebrafish reference genome GRCz11 using HiSAT2 version 2.1.0. Reads per million RPM were calculated using the Subread ver. 1.6.4. Genome build: GRCz11 Supplementary files format and content: Reads per million RPM values for each sample.", "osteoblast derived extracellular vesicles", null, "Total RNAs were extracted from sorted cells using RNeasy Mini Kit QIAGEN. Cells were directly sorted in a lysis buffer containing 1 \u03bcg/mL of polyinosinic polycytidylic acid  and total RNA was extracted using RNeasy Mini Kit. Reverse transcription RT was performed using Super Script III Thermo Fisher Scientific and an RT primer  which contains oligo dT  T7 promoter  and PCR target region sequences. post digestion of remaining RT primers by exonuclease I Takara  a poly A tail was added to the 3\u2019 ends of the first strand cDNAs using terminal transferase Sigma. The second strand DNA was then synthesized using MightyAmp DNA polymerase Thermo Fisher Scientific and a tagging primer  which contains oligo dT and PCR target region sequences. PCR amplification was performed using a suppression primer  which allow to amplify small size DNA that contains complementary sequences at both ends of the template DNA. The amplified double strand cDNA was purified using QIAquick PCR Purification Kit Qiagen. Library preparation was performed using Nextera XT DNA Library Preparation Kit illumina.", null, "age:adult|genotype:wild type|tissue:Sorted from the fractured sclae|cell type:osteoblast derived extracellular vesicles|molecule type:Amplified RNA", "GSM3942348", "GSM3942348: EV 2; Danio rerio; RNA Seq", "GSM3942348", null, "1", "Total RNAs were extracted from sorted cells using RNeasy Mini Kit QIAGEN. Cells were directly sorted in a lysis buffer containing 1 \u03bcg/mL of polyinosinic polycytidylic acid  and total RNA was extracted using RNeasy Mini Kit. Reverse transcription RT was performed using Super Script III Thermo Fisher Scientific and an RT primer  which contains oligo dT  T7 promoter  and PCR target region sequences. post digestion of remaining RT primers by exonuclease I Takara  a poly A tail was added to the three prime ends of the first strand cDNAs using terminal transferase Sigma. The second strand DNA was then synthesized using MightyAmp DNA polymerase Thermo Fisher Scientific and a tagging primer  which contains oligo dT and PCR target region sequences. PCR amplification was performed using a suppression primer  which allow to amplify small size DNA that contains complementary sequences at both ends of the template DNA. The amplified double strand cDNA was purified using QIAquick PCR Purification Kit Qiagen. Library preparation was performed using Nextera XT DNA Library Preparation Kit illumina.", null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 500", null, "SRP214773", null, null, "EV_2_R1.fastq.gz EV_2_R2.fastq.gz", "fastq fastq", 6759957000.0, 22533190.0, "GSM3942348 r1", "0:150 1:150", "A:1706593126;C:1699056754;G:1646763192;T:1707067337;N:476591", 150, 150, null, null, 1706593126, 1699056754, 1646763192, 1707067337, 476591, "SRX6451056", "SRS5104327", "SRA921382", "GEO", "Kanazawa University", 2, 0.94501, 0.94549, 0.02885, 0.03033, 0.80458, 0.80957, 0.48799, 0.50474, 150, 150, "B", "B", "biological fallback assumption", "illumina", "nextseq", "3prime", "poly_a", "nextera", "sc", "single_cell_plate", "quartzseq", null, "Japan", "2019-07-16", "Adult", "Adult", "Bone or Cartilage", "Skeletal Element"], [53149, "SRR9692021", "SRX6451055", "SRS5104326", "SRP214773", "PRJNA554788", "Gene expression analysis in the zebrafish fractured scale", "GSE134330", "Transcriptome Analysis", "Bone is a connective tissue which undergoes continuous remodeling  including bone resorption and formation by osteoclasts OCs and osteoblasts OBs  respectively. Recently  extracellular vesicles EVs are increasingly appreciated as a regulator of intercellular communication between OCs and OBs. The zebrafish scale is a thin membranous bone embedded in the skin and consists of OBs  OCs  and bone matrix  providing an elegant model to visualize OC OB communication. Here  we developed a double transgenic zebrafish line  trap:GFP; osterix:mCherry  which expresses GFP and mCherry under the control of the OC specific trap tartrate resistant acid phosphatase and OB specific osterix enhancer  respectively. Utilizing this double transgenic line  in combination with Hoechst 33342 Hoe staining  we isolated four distinct fractions from the scale at 1 day post fracture by flow cytometry  GFP\u2013 mCh+ Hoehigh OB fraction  GFPlow mCh+ Hoehigh OC precursor pOC fraction  GFPhigh Hoehigh mature OC mOC fraction  and mCh+ Hoe\u2013 OB derived extracellular vesicle EV fraction. In this study  we have performed RNA seq analysis using these four fractions to examine the gene expression profile of each  fraction. Overall design: mRNA profiles of isolated OBs  pOCs   mOCs  and OB derived EVs in the zebrafish fractured scales were generated by Quartz seq using Illumina NextSeq500.", null, "pubmed:32327701", null, "EV 1", "GSM3942347", null, "source name:osteoblast derived extracellular vesicles|age:adult|genotype:wild type|tissue:Sorted from the fractured sclae|cell type:osteoblast derived extracellular vesicles|molecule type:Amplified RNA", "EV 1", "Illumina RTA software was used for base calling. Sequence reads were mapped to the zebrafish reference genome GRCz11 using HiSAT2 version 2.1.0. Reads per million RPM were calculated using the Subread ver. 1.6.4. Genome build: GRCz11 Supplementary files format and content: Reads per million RPM values for each sample.", "osteoblast derived extracellular vesicles", null, "Total RNAs were extracted from sorted cells using RNeasy Mini Kit QIAGEN. Cells were directly sorted in a lysis buffer containing 1 \u03bcg/mL of polyinosinic polycytidylic acid  and total RNA was extracted using RNeasy Mini Kit. Reverse transcription RT was performed using Super Script III Thermo Fisher Scientific and an RT primer  which contains oligo dT  T7 promoter  and PCR target region sequences. post digestion of remaining RT primers by exonuclease I Takara  a poly A tail was added to the 3\u2019 ends of the first strand cDNAs using terminal transferase Sigma. The second strand DNA was then synthesized using MightyAmp DNA polymerase Thermo Fisher Scientific and a tagging primer  which contains oligo dT and PCR target region sequences. PCR amplification was performed using a suppression primer  which allow to amplify small size DNA that contains complementary sequences at both ends of the template DNA. The amplified double strand cDNA was purified using QIAquick PCR Purification Kit Qiagen. Library preparation was performed using Nextera XT DNA Library Preparation Kit illumina.", null, "age:adult|genotype:wild type|tissue:Sorted from the fractured sclae|cell type:osteoblast derived extracellular vesicles|molecule type:Amplified RNA", "GSM3942347", "GSM3942347: EV 1; Danio rerio; RNA Seq", "GSM3942347", null, "1", "Total RNAs were extracted from sorted cells using RNeasy Mini Kit QIAGEN. Cells were directly sorted in a lysis buffer containing 1 \u03bcg/mL of polyinosinic polycytidylic acid  and total RNA was extracted using RNeasy Mini Kit. Reverse transcription RT was performed using Super Script III Thermo Fisher Scientific and an RT primer  which contains oligo dT  T7 promoter  and PCR target region sequences. post digestion of remaining RT primers by exonuclease I Takara  a poly A tail was added to the three prime ends of the first strand cDNAs using terminal transferase Sigma. The second strand DNA was then synthesized using MightyAmp DNA polymerase Thermo Fisher Scientific and a tagging primer  which contains oligo dT and PCR target region sequences. PCR amplification was performed using a suppression primer  which allow to amplify small size DNA that contains complementary sequences at both ends of the template DNA. The amplified double strand cDNA was purified using QIAquick PCR Purification Kit Qiagen. Library preparation was performed using Nextera XT DNA Library Preparation Kit illumina.", null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 500", null, "SRP214773", null, null, "EV_1_R1.fastq.gz EV_1_R2.fastq.gz", "fastq fastq", 6368980200.0, 21229934.0, "GSM3942347 r1", "0:150 1:150", "A:1609224577;C:1593521490;G:1569729629;T:1596056057;N:448447", 150, 150, null, null, 1609224577, 1593521490, 1569729629, 1596056057, 448447, "SRX6451055", "SRS5104326", "SRA921382", "GEO", "Kanazawa University", 2, 0.93738, 0.93601, 0.02919, 0.03087, 0.79669, 0.80312, 0.50627, 0.50567, 150, 150, "B", "B", "biological fallback assumption", "illumina", "nextseq", "3prime", "poly_a", "nextera", "sc", "single_cell_plate", "quartzseq", null, "Japan", "2019-07-16", "Adult", "Adult", "Bone or Cartilage", "Skeletal Element"], [53154, "SRR9692016", "SRX6451050", "SRS5104321", "SRP214773", "PRJNA554788", "Gene expression analysis in the zebrafish fractured scale", "GSE134330", "Transcriptome Analysis", "Bone is a connective tissue which undergoes continuous remodeling  including bone resorption and formation by osteoclasts OCs and osteoblasts OBs  respectively. Recently  extracellular vesicles EVs are increasingly appreciated as a regulator of intercellular communication between OCs and OBs. The zebrafish scale is a thin membranous bone embedded in the skin and consists of OBs  OCs  and bone matrix  providing an elegant model to visualize OC OB communication. Here  we developed a double transgenic zebrafish line  trap:GFP; osterix:mCherry  which expresses GFP and mCherry under the control of the OC specific trap tartrate resistant acid phosphatase and OB specific osterix enhancer  respectively. Utilizing this double transgenic line  in combination with Hoechst 33342 Hoe staining  we isolated four distinct fractions from the scale at 1 day post fracture by flow cytometry  GFP\u2013 mCh+ Hoehigh OB fraction  GFPlow mCh+ Hoehigh OC precursor pOC fraction  GFPhigh Hoehigh mature OC mOC fraction  and mCh+ Hoe\u2013 OB derived extracellular vesicle EV fraction. In this study  we have performed RNA seq analysis using these four fractions to examine the gene expression profile of each  fraction. Overall design: mRNA profiles of isolated OBs  pOCs   mOCs  and OB derived EVs in the zebrafish fractured scales were generated by Quartz seq using Illumina NextSeq500.", null, "pubmed:32327701", null, "OB 2", "GSM3942342", null, "source name:osteoblasts|age:adult|genotype:wild type|tissue:Sorted from the fractured sclae|cell type:osteoblasts|molecule type:Amplified RNA", "OB 2", "Illumina RTA software was used for base calling. Sequence reads were mapped to the zebrafish reference genome GRCz11 using HiSAT2 version 2.1.0. Reads per million RPM were calculated using the Subread ver. 1.6.4. Genome build: GRCz11 Supplementary files format and content: Reads per million RPM values for each sample.", "osteoblasts", null, "Total RNAs were extracted from sorted cells using RNeasy Mini Kit QIAGEN. Cells were directly sorted in a lysis buffer containing 1 \u03bcg/mL of polyinosinic polycytidylic acid  and total RNA was extracted using RNeasy Mini Kit. Reverse transcription RT was performed using Super Script III Thermo Fisher Scientific and an RT primer  which contains oligo dT  T7 promoter  and PCR target region sequences. post digestion of remaining RT primers by exonuclease I Takara  a poly A tail was added to the 3\u2019 ends of the first strand cDNAs using terminal transferase Sigma. The second strand DNA was then synthesized using MightyAmp DNA polymerase Thermo Fisher Scientific and a tagging primer  which contains oligo dT and PCR target region sequences. PCR amplification was performed using a suppression primer  which allow to amplify small size DNA that contains complementary sequences at both ends of the template DNA. The amplified double strand cDNA was purified using QIAquick PCR Purification Kit Qiagen. Library preparation was performed using Nextera XT DNA Library Preparation Kit illumina.", null, "age:adult|genotype:wild type|tissue:Sorted from the fractured sclae|cell type:osteoblasts|molecule type:Amplified RNA", "GSM3942342", "GSM3942342: OB 2; Danio rerio; RNA Seq", "GSM3942342", null, "1", "Total RNAs were extracted from sorted cells using RNeasy Mini Kit QIAGEN. Cells were directly sorted in a lysis buffer containing 1 \u03bcg/mL of polyinosinic polycytidylic acid  and total RNA was extracted using RNeasy Mini Kit. Reverse transcription RT was performed using Super Script III Thermo Fisher Scientific and an RT primer  which contains oligo dT  T7 promoter  and PCR target region sequences. post digestion of remaining RT primers by exonuclease I Takara  a poly A tail was added to the three prime ends of the first strand cDNAs using terminal transferase Sigma. The second strand DNA was then synthesized using MightyAmp DNA polymerase Thermo Fisher Scientific and a tagging primer  which contains oligo dT and PCR target region sequences. PCR amplification was performed using a suppression primer  which allow to amplify small size DNA that contains complementary sequences at both ends of the template DNA. The amplified double strand cDNA was purified using QIAquick PCR Purification Kit Qiagen. Library preparation was performed using Nextera XT DNA Library Preparation Kit illumina.", null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 500", null, "SRP214773", null, null, "OB_2_R1.fastq.gz OB_2_R2.fastq.gz", "fastq fastq", 4566907200.0, 15223024.0, "GSM3942342 r1", "0:150 1:150", "A:1133280984;C:1162119484;G:1119861724;T:1151514772;N:130236", 150, 150, null, null, 1133280984, 1162119484, 1119861724, 1151514772, 130236, "SRX6451050", "SRS5104321", "SRA921382", "GEO", "Kanazawa University", 2, 0.9502, 0.9473, 0.0382, 0.04036, 0.77853, 0.78719, 0.47336, 0.4862, 150, 150, "B", "B", "biological fallback assumption", "illumina", "nextseq", "3prime", "poly_a", "nextera", "sc", "single_cell_plate", "quartzseq", null, "Japan", "2019-07-16", "Adult", "Adult", "Bone or Cartilage", "Skeletal Element"], [53155, "SRR9692015", "SRX6451049", "SRS5104320", "SRP214773", "PRJNA554788", "Gene expression analysis in the zebrafish fractured scale", "GSE134330", "Transcriptome Analysis", "Bone is a connective tissue which undergoes continuous remodeling  including bone resorption and formation by osteoclasts OCs and osteoblasts OBs  respectively. Recently  extracellular vesicles EVs are increasingly appreciated as a regulator of intercellular communication between OCs and OBs. The zebrafish scale is a thin membranous bone embedded in the skin and consists of OBs  OCs  and bone matrix  providing an elegant model to visualize OC OB communication. Here  we developed a double transgenic zebrafish line  trap:GFP; osterix:mCherry  which expresses GFP and mCherry under the control of the OC specific trap tartrate resistant acid phosphatase and OB specific osterix enhancer  respectively. Utilizing this double transgenic line  in combination with Hoechst 33342 Hoe staining  we isolated four distinct fractions from the scale at 1 day post fracture by flow cytometry  GFP\u2013 mCh+ Hoehigh OB fraction  GFPlow mCh+ Hoehigh OC precursor pOC fraction  GFPhigh Hoehigh mature OC mOC fraction  and mCh+ Hoe\u2013 OB derived extracellular vesicle EV fraction. In this study  we have performed RNA seq analysis using these four fractions to examine the gene expression profile of each  fraction. Overall design: mRNA profiles of isolated OBs  pOCs   mOCs  and OB derived EVs in the zebrafish fractured scales were generated by Quartz seq using Illumina NextSeq500.", null, "pubmed:32327701", null, "OB 1", "GSM3942341", null, "source name:osteoblasts|age:adult|genotype:wild type|tissue:Sorted from the fractured sclae|cell type:osteoblasts|molecule type:Amplified RNA", "OB 1", "Illumina RTA software was used for base calling. Sequence reads were mapped to the zebrafish reference genome GRCz11 using HiSAT2 version 2.1.0. Reads per million RPM were calculated using the Subread ver. 1.6.4. Genome build: GRCz11 Supplementary files format and content: Reads per million RPM values for each sample.", "osteoblasts", null, "Total RNAs were extracted from sorted cells using RNeasy Mini Kit QIAGEN. Cells were directly sorted in a lysis buffer containing 1 \u03bcg/mL of polyinosinic polycytidylic acid  and total RNA was extracted using RNeasy Mini Kit. Reverse transcription RT was performed using Super Script III Thermo Fisher Scientific and an RT primer  which contains oligo dT  T7 promoter  and PCR target region sequences. post digestion of remaining RT primers by exonuclease I Takara  a poly A tail was added to the 3\u2019 ends of the first strand cDNAs using terminal transferase Sigma. The second strand DNA was then synthesized using MightyAmp DNA polymerase Thermo Fisher Scientific and a tagging primer  which contains oligo dT and PCR target region sequences. PCR amplification was performed using a suppression primer  which allow to amplify small size DNA that contains complementary sequences at both ends of the template DNA. The amplified double strand cDNA was purified using QIAquick PCR Purification Kit Qiagen. Library preparation was performed using Nextera XT DNA Library Preparation Kit illumina.", null, "age:adult|genotype:wild type|tissue:Sorted from the fractured sclae|cell type:osteoblasts|molecule type:Amplified RNA", "GSM3942341", "GSM3942341: OB 1; Danio rerio; RNA Seq", "GSM3942341", null, "1", "Total RNAs were extracted from sorted cells using RNeasy Mini Kit QIAGEN. Cells were directly sorted in a lysis buffer containing 1 \u03bcg/mL of polyinosinic polycytidylic acid  and total RNA was extracted using RNeasy Mini Kit. Reverse transcription RT was performed using Super Script III Thermo Fisher Scientific and an RT primer  which contains oligo dT  T7 promoter  and PCR target region sequences. post digestion of remaining RT primers by exonuclease I Takara  a poly A tail was added to the three prime ends of the first strand cDNAs using terminal transferase Sigma. The second strand DNA was then synthesized using MightyAmp DNA polymerase Thermo Fisher Scientific and a tagging primer  which contains oligo dT and PCR target region sequences. PCR amplification was performed using a suppression primer  which allow to amplify small size DNA that contains complementary sequences at both ends of the template DNA. The amplified double strand cDNA was purified using QIAquick PCR Purification Kit Qiagen. Library preparation was performed using Nextera XT DNA Library Preparation Kit illumina.", null, "RNA-Seq", "TRANSCRIPTOMIC", "cDNA", "PAIRED", "ILLUMINA", "NextSeq 500", null, "SRP214773", null, null, "OB_1_R1.fastq.gz OB_1_R2.fastq.gz", "fastq fastq", 3924215700.0, 13080719.0, "GSM3942341 r1", "0:150 1:150", "A:983923789;C:989882668;G:965519773;T:984780602;N:108868", 150, 150, null, null, 983923789, 989882668, 965519773, 984780602, 108868, "SRX6451049", "SRS5104320", "SRA921382", "GEO", "Kanazawa University", 2, 0.95148, 0.94857, 0.03822, 0.04077, 0.77705, 0.78729, 0.48248, 0.49117, 150, 150, "B", "B", "biological fallback assumption", "illumina", "nextseq", "3prime", "poly_a", "nextera", "sc", "single_cell_plate", "quartzseq", null, "Japan", "2019-07-16", "Adult", "Adult", "Bone or Cartilage", "Skeletal Element"], [74701, "SRR24003726", "SRX19807142", "SRS17168877", "SRP429947", "PRJNA949785", "Sequencing of zebrafish bone tissues", "PRJNA949785", "Other", "Transcriptomics was used to investigate sensitive genes associated with exercise improved bone mass", null, null, null, null, "eight month zebrafish bone tissues", null, "breed:Not applicable|age:8 month|sex:male|tissue:b1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of b1 tissue in zebrafish", "case2 4", "case2 4", "Qualified total RNA was purified by DNase I  and mRNA was enriched by adding OligodT  attached magnetic beads. Subsequently  fragment buffer was added to cleave the mRNA into small   fragments. Fragmented mRNA was used as a template for reverse transcription to generate the first  cDNA strand  and then the second cDNA strand was generated. End repair and adaptor ligation were  performed by adding A tailing Mix and RNA Index Adapters  and then PCR amplification was performed. Single strand circle DNA library was obtained by recovering and cycling the PCR products.  The final library was obtained by purifying the linear DNA molecules that were not cycled. The quality  of the final library was tested. The qualified library was replicated by rolling circle replication to form  DNA nanospheres DNBs. DNBs were added to the mesh holes of a nanochip using high density DNA nanochip technology. Subsequently  sequencing was performed on the BGIISEQ500 platform  BGI Shenzhen  Guangdong  China using combined probe anchored polymerisation.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PCR", "PAIRED", "BGISEQ", "BGISEQ-500", null, "SRP429947", null, null, "case2_4_1.fq.gz case2_4_2.fq.gz", "fastq fastq", 6631325400.0, 22104418.0, "case2 4 1.fq.gz", "0:150 1:150", "A:1790451511;C:1523987494;G:1517049214;T:1799837181;N:0", 150, 150, null, null, 1790451511, 1523987494, 1517049214, 1799837181, 0, "SRX19807142", "SRS17168877", "SRA1612461", "Sun Yat-sen University|Department of Epidemiology, School of Public Healt", "Sun Yat-sen University", 2, 0.94202, 0.94054, 0.06448, 0.06393, 0.71415, 0.71445, 0.52801, 0.52947, 150, 150, "B", "B", "biological fallback assumption", "bgi", "bgi", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2023-03-29", "Adult", "Adult", "Bone or Cartilage", "Skeletal Element"], [74702, "SRR24003727", "SRX19807141", "SRS17168877", "SRP429947", "PRJNA949785", "Sequencing of zebrafish bone tissues", "PRJNA949785", "Other", "Transcriptomics was used to investigate sensitive genes associated with exercise improved bone mass", null, null, null, null, "eight month zebrafish bone tissues", null, "breed:Not applicable|age:8 month|sex:male|tissue:b1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of b1 tissue in zebrafish", "case2 3", "case2 3", "Qualified total RNA was purified by DNase I  and mRNA was enriched by adding OligodT  attached magnetic beads. Subsequently  fragment buffer was added to cleave the mRNA into small   fragments. Fragmented mRNA was used as a template for reverse transcription to generate the first  cDNA strand  and then the second cDNA strand was generated. End repair and adaptor ligation were  performed by adding A tailing Mix and RNA Index Adapters  and then PCR amplification was performed. Single strand circle DNA library was obtained by recovering and cycling the PCR products.  The final library was obtained by purifying the linear DNA molecules that were not cycled. The quality  of the final library was tested. The qualified library was replicated by rolling circle replication to form  DNA nanospheres DNBs. DNBs were added to the mesh holes of a nanochip using high density DNA nanochip technology. Subsequently  sequencing was performed on the BGIISEQ500 platform  BGI Shenzhen  Guangdong  China using combined probe anchored polymerisation.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PCR", "PAIRED", "BGISEQ", "BGISEQ-500", null, "SRP429947", null, null, "case2_3_1.fq.gz case2_3_2.fq.gz", "fastq fastq", 6706743300.0, 22355811.0, "case2 3 1.fq.gz", "0:150 1:150", "A:1845685627;C:1507725453;G:1499541624;T:1853790596;N:0", 150, 150, null, null, 1845685627, 1507725453, 1499541624, 1853790596, 0, "SRX19807141", "SRS17168877", "SRA1612461", "Sun Yat-sen University|Department of Epidemiology, School of Public Healt", "Sun Yat-sen University", 2, 0.94268, 0.93594, 0.08009, 0.07937, 0.70402, 0.70441, 0.4556, 0.48818, 150, 150, "B", "B", "biological fallback assumption", "bgi", "bgi", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2023-03-29", "Adult", "Adult", "Bone or Cartilage", "Skeletal Element"], [74703, "SRR24003728", "SRX19807140", "SRS17168877", "SRP429947", "PRJNA949785", "Sequencing of zebrafish bone tissues", "PRJNA949785", "Other", "Transcriptomics was used to investigate sensitive genes associated with exercise improved bone mass", null, null, null, null, "eight month zebrafish bone tissues", null, "breed:Not applicable|age:8 month|sex:male|tissue:b1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of b1 tissue in zebrafish", "case2 2", "case2 2", "Qualified total RNA was purified by DNase I  and mRNA was enriched by adding OligodT  attached magnetic beads. Subsequently  fragment buffer was added to cleave the mRNA into small   fragments. Fragmented mRNA was used as a template for reverse transcription to generate the first  cDNA strand  and then the second cDNA strand was generated. End repair and adaptor ligation were  performed by adding A tailing Mix and RNA Index Adapters  and then PCR amplification was performed. Single strand circle DNA library was obtained by recovering and cycling the PCR products.  The final library was obtained by purifying the linear DNA molecules that were not cycled. The quality  of the final library was tested. The qualified library was replicated by rolling circle replication to form  DNA nanospheres DNBs. DNBs were added to the mesh holes of a nanochip using high density DNA nanochip technology. Subsequently  sequencing was performed on the BGIISEQ500 platform  BGI Shenzhen  Guangdong  China using combined probe anchored polymerisation.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PCR", "PAIRED", "BGISEQ", "BGISEQ-500", null, "SRP429947", null, null, "case2_2_1.fq.gz case2_2_2.fq.gz", "fastq fastq", 6677092200.0, 22256974.0, "case2 2 1.fq.gz", "0:150 1:150", "A:1864795230;C:1474224567;G:1465199644;T:1872872759;N:0", 150, 150, null, null, 1864795230, 1474224567, 1465199644, 1872872759, 0, "SRX19807140", "SRS17168877", "SRA1612461", "Sun Yat-sen University|Department of Epidemiology, School of Public Healt", "Sun Yat-sen University", 2, 0.93359, 0.92743, 0.0959, 0.09583, 0.69089, 0.69234, 0.48419, 0.48121, 150, 150, "B", "B", "biological fallback assumption", "bgi", "bgi", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2023-03-29", "Adult", "Adult", "Bone or Cartilage", "Skeletal Element"], [74704, "SRR24003729", "SRX19807139", "SRS17168877", "SRP429947", "PRJNA949785", "Sequencing of zebrafish bone tissues", "PRJNA949785", "Other", "Transcriptomics was used to investigate sensitive genes associated with exercise improved bone mass", null, null, null, null, "eight month zebrafish bone tissues", null, "breed:Not applicable|age:8 month|sex:male|tissue:b1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of b1 tissue in zebrafish", "case2 1", "case2 1", "Qualified total RNA was purified by DNase I  and mRNA was enriched by adding OligodT  attached magnetic beads. Subsequently  fragment buffer was added to cleave the mRNA into small   fragments. Fragmented mRNA was used as a template for reverse transcription to generate the first  cDNA strand  and then the second cDNA strand was generated. End repair and adaptor ligation were  performed by adding A tailing Mix and RNA Index Adapters  and then PCR amplification was performed. Single strand circle DNA library was obtained by recovering and cycling the PCR products.  The final library was obtained by purifying the linear DNA molecules that were not cycled. The quality  of the final library was tested. The qualified library was replicated by rolling circle replication to form  DNA nanospheres DNBs. DNBs were added to the mesh holes of a nanochip using high density DNA nanochip technology. Subsequently  sequencing was performed on the BGIISEQ500 platform  BGI Shenzhen  Guangdong  China using combined probe anchored polymerisation.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PCR", "PAIRED", "BGISEQ", "BGISEQ-500", null, "SRP429947", null, null, "case2_1_1.fq.gz case2_1_2.fq.gz", "fastq fastq", 6644478600.0, 22148262.0, "case2 1 1.fq.gz", "0:150 1:150", "A:1863814645;C:1460246773;G:1453279946;T:1867137236;N:0", 150, 150, null, null, 1863814645, 1460246773, 1453279946, 1867137236, 0, "SRX19807139", "SRS17168877", "SRA1612461", "Sun Yat-sen University|Department of Epidemiology, School of Public Healt", "Sun Yat-sen University", 2, 0.93932, 0.9379, 0.09722, 0.09709, 0.71985, 0.71873, 0.49646, 0.5522, 150, 150, "B", "B", "biological fallback assumption", "bgi", "bgi", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2023-03-29", "Adult", "Adult", "Bone or Cartilage", "Skeletal Element"], [74705, "SRR24003730", "SRX19807138", "SRS17168877", "SRP429947", "PRJNA949785", "Sequencing of zebrafish bone tissues", "PRJNA949785", "Other", "Transcriptomics was used to investigate sensitive genes associated with exercise improved bone mass", null, null, null, null, "eight month zebrafish bone tissues", null, "breed:Not applicable|age:8 month|sex:male|tissue:b1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of b1 tissue in zebrafish", "control2 6", "control2 6", "Qualified total RNA was purified by DNase I  and mRNA was enriched by adding OligodT  attached magnetic beads. Subsequently  fragment buffer was added to cleave the mRNA into small   fragments. Fragmented mRNA was used as a template for reverse transcription to generate the first  cDNA strand  and then the second cDNA strand was generated. End repair and adaptor ligation were  performed by adding A tailing Mix and RNA Index Adapters  and then PCR amplification was performed. Single strand circle DNA library was obtained by recovering and cycling the PCR products.  The final library was obtained by purifying the linear DNA molecules that were not cycled. The quality  of the final library was tested. The qualified library was replicated by rolling circle replication to form  DNA nanospheres DNBs. DNBs were added to the mesh holes of a nanochip using high density DNA nanochip technology. Subsequently  sequencing was performed on the BGIISEQ500 platform  BGI Shenzhen  Guangdong  China using combined probe anchored polymerisation.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PCR", "PAIRED", "BGISEQ", "BGISEQ-500", null, "SRP429947", null, null, "control2_6_1.fq.gz control2_6_2.fq.gz", "fastq fastq", 6817849200.0, 22726164.0, "control2 6 1.fq.gz", "0:150 1:150", "A:1916198776;C:1480570561;G:1501234091;T:1919845772;N:0", 150, 150, null, null, 1916198776, 1480570561, 1501234091, 1919845772, 0, "SRX19807138", "SRS17168877", "SRA1612461", "Sun Yat-sen University|Department of Epidemiology, School of Public Healt", "Sun Yat-sen University", 2, 0.94697, 0.9369, 0.06934, 0.06812, 0.76824, 0.77114, 0.49005, 0.54882, 150, 150, "B", "B", "biological fallback assumption", "bgi", "bgi", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2023-03-29", "Adult", "Adult", "Bone or Cartilage", "Skeletal Element"], [74706, "SRR24003731", "SRX19807137", "SRS17168877", "SRP429947", "PRJNA949785", "Sequencing of zebrafish bone tissues", "PRJNA949785", "Other", "Transcriptomics was used to investigate sensitive genes associated with exercise improved bone mass", null, null, null, null, "eight month zebrafish bone tissues", null, "breed:Not applicable|age:8 month|sex:male|tissue:b1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of b1 tissue in zebrafish", "control2 5", "control2 5", "Qualified total RNA was purified by DNase I  and mRNA was enriched by adding OligodT  attached magnetic beads. Subsequently  fragment buffer was added to cleave the mRNA into small   fragments. Fragmented mRNA was used as a template for reverse transcription to generate the first  cDNA strand  and then the second cDNA strand was generated. End repair and adaptor ligation were  performed by adding A tailing Mix and RNA Index Adapters  and then PCR amplification was performed. Single strand circle DNA library was obtained by recovering and cycling the PCR products.  The final library was obtained by purifying the linear DNA molecules that were not cycled. The quality  of the final library was tested. The qualified library was replicated by rolling circle replication to form  DNA nanospheres DNBs. DNBs were added to the mesh holes of a nanochip using high density DNA nanochip technology. Subsequently  sequencing was performed on the BGIISEQ500 platform  BGI Shenzhen  Guangdong  China using combined probe anchored polymerisation.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PCR", "PAIRED", "BGISEQ", "BGISEQ-500", null, "SRP429947", null, null, "control2_5_1.fq.gz control2_5_2.fq.gz", "fastq fastq", 6730171500.0, 22433905.0, "control2 5 1.fq.gz", "0:150 1:150", "A:1881763818;C:1469481635;G:1493477302;T:1885448745;N:0", 150, 150, null, null, 1881763818, 1469481635, 1493477302, 1885448745, 0, "SRX19807137", "SRS17168877", "SRA1612461", "Sun Yat-sen University|Department of Epidemiology, School of Public Healt", "Sun Yat-sen University", 2, 0.9399, 0.92905, 0.0832, 0.08122, 0.70993, 0.71228, 0.54991, 0.58952, 150, 150, "B", "B", "biological fallback assumption", "bgi", "bgi", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2023-03-29", "Adult", "Adult", "Bone or Cartilage", "Skeletal Element"], [74707, "SRR24003732", "SRX19807136", "SRS17168877", "SRP429947", "PRJNA949785", "Sequencing of zebrafish bone tissues", "PRJNA949785", "Other", "Transcriptomics was used to investigate sensitive genes associated with exercise improved bone mass", null, null, null, null, "eight month zebrafish bone tissues", null, "breed:Not applicable|age:8 month|sex:male|tissue:b1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of b1 tissue in zebrafish", "control2 4", "control2 4", "Qualified total RNA was purified by DNase I  and mRNA was enriched by adding OligodT  attached magnetic beads. Subsequently  fragment buffer was added to cleave the mRNA into small   fragments. Fragmented mRNA was used as a template for reverse transcription to generate the first  cDNA strand  and then the second cDNA strand was generated. End repair and adaptor ligation were  performed by adding A tailing Mix and RNA Index Adapters  and then PCR amplification was performed. Single strand circle DNA library was obtained by recovering and cycling the PCR products.  The final library was obtained by purifying the linear DNA molecules that were not cycled. The quality  of the final library was tested. The qualified library was replicated by rolling circle replication to form  DNA nanospheres DNBs. DNBs were added to the mesh holes of a nanochip using high density DNA nanochip technology. Subsequently  sequencing was performed on the BGIISEQ500 platform  BGI Shenzhen  Guangdong  China using combined probe anchored polymerisation.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PCR", "PAIRED", "BGISEQ", "BGISEQ-500", null, "SRP429947", null, null, "control2_4_1.fq.gz control2_4_2.fq.gz", "fastq fastq", 6660686100.0, 22202287.0, "control2 4 1.fq.gz", "0:150 1:150", "A:1846205332;C:1486328129;G:1477889862;T:1850262777;N:0", 150, 150, null, null, 1846205332, 1486328129, 1477889862, 1850262777, 0, "SRX19807136", "SRS17168877", "SRA1612461", "Sun Yat-sen University|Department of Epidemiology, School of Public Healt", "Sun Yat-sen University", 2, 0.94251, 0.94001, 0.08206, 0.082, 0.69483, 0.69524, 0.49193, 0.49602, 150, 150, "B", "B", "biological fallback assumption", "bgi", "bgi", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2023-03-29", "Adult", "Adult", "Bone or Cartilage", "Skeletal Element"], [74708, "SRR24003733", "SRX19807135", "SRS17168877", "SRP429947", "PRJNA949785", "Sequencing of zebrafish bone tissues", "PRJNA949785", "Other", "Transcriptomics was used to investigate sensitive genes associated with exercise improved bone mass", null, null, null, null, "eight month zebrafish bone tissues", null, "breed:Not applicable|age:8 month|sex:male|tissue:b1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of b1 tissue in zebrafish", "control2 3", "control2 3", "Qualified total RNA was purified by DNase I  and mRNA was enriched by adding OligodT  attached magnetic beads. Subsequently  fragment buffer was added to cleave the mRNA into small   fragments. Fragmented mRNA was used as a template for reverse transcription to generate the first  cDNA strand  and then the second cDNA strand was generated. End repair and adaptor ligation were  performed by adding A tailing Mix and RNA Index Adapters  and then PCR amplification was performed. Single strand circle DNA library was obtained by recovering and cycling the PCR products.  The final library was obtained by purifying the linear DNA molecules that were not cycled. The quality  of the final library was tested. The qualified library was replicated by rolling circle replication to form  DNA nanospheres DNBs. DNBs were added to the mesh holes of a nanochip using high density DNA nanochip technology. Subsequently  sequencing was performed on the BGIISEQ500 platform  BGI Shenzhen  Guangdong  China using combined probe anchored polymerisation.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PCR", "PAIRED", "BGISEQ", "BGISEQ-500", null, "SRP429947", null, null, "control2_3_1.fq.gz control2_3_2.fq.gz", "fastq fastq", 6683569500.0, 22278565.0, "control2 3 1.fq.gz", "0:150 1:150", "A:1846755716;C:1494194697;G:1482717957;T:1859901130;N:0", 150, 150, null, null, 1846755716, 1494194697, 1482717957, 1859901130, 0, "SRX19807135", "SRS17168877", "SRA1612461", "Sun Yat-sen University|Department of Epidemiology, School of Public Healt", "Sun Yat-sen University", 2, 0.93255, 0.93106, 0.10717, 0.10731, 0.67665, 0.67791, 0.54876, 0.52297, 150, 150, "B", "B", "biological fallback assumption", "bgi", "bgi", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2023-03-29", "Adult", "Adult", "Bone or Cartilage", "Skeletal Element"], [74709, "SRR24003734", "SRX19807134", "SRS17168877", "SRP429947", "PRJNA949785", "Sequencing of zebrafish bone tissues", "PRJNA949785", "Other", "Transcriptomics was used to investigate sensitive genes associated with exercise improved bone mass", null, null, null, null, "eight month zebrafish bone tissues", null, "breed:Not applicable|age:8 month|sex:male|tissue:b1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of b1 tissue in zebrafish", "case2 7", "case2 7", "Qualified total RNA was purified by DNase I  and mRNA was enriched by adding OligodT  attached magnetic beads. Subsequently  fragment buffer was added to cleave the mRNA into small   fragments. Fragmented mRNA was used as a template for reverse transcription to generate the first  cDNA strand  and then the second cDNA strand was generated. End repair and adaptor ligation were  performed by adding A tailing Mix and RNA Index Adapters  and then PCR amplification was performed. Single strand circle DNA library was obtained by recovering and cycling the PCR products.  The final library was obtained by purifying the linear DNA molecules that were not cycled. The quality  of the final library was tested. The qualified library was replicated by rolling circle replication to form  DNA nanospheres DNBs. DNBs were added to the mesh holes of a nanochip using high density DNA nanochip technology. Subsequently  sequencing was performed on the BGIISEQ500 platform  BGI Shenzhen  Guangdong  China using combined probe anchored polymerisation.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PCR", "PAIRED", "BGISEQ", "BGISEQ-500", null, "SRP429947", null, null, "case2_7_1.fq.gz case2_7_2.fq.gz", "fastq fastq", 6770748000.0, 22569160.0, "case2 7 1.fq.gz", "0:150 1:150", "A:1918448687;C:1455131518;G:1474277741;T:1922890054;N:0", 150, 150, null, null, 1918448687, 1455131518, 1474277741, 1922890054, 0, "SRX19807134", "SRS17168877", "SRA1612461", "Sun Yat-sen University|Department of Epidemiology, School of Public Healt", "Sun Yat-sen University", 2, 0.94025, 0.92953, 0.08709, 0.0851, 0.73864, 0.73914, 0.5716, 0.56037, 150, 150, "B", "B", "biological fallback assumption", "bgi", "bgi", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2023-03-29", "Adult", "Adult", "Bone or Cartilage", "Skeletal Element"], [74710, "SRR24003735", "SRX19807133", "SRS17168877", "SRP429947", "PRJNA949785", "Sequencing of zebrafish bone tissues", "PRJNA949785", "Other", "Transcriptomics was used to investigate sensitive genes associated with exercise improved bone mass", null, null, null, null, "eight month zebrafish bone tissues", null, "breed:Not applicable|age:8 month|sex:male|tissue:b1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of b1 tissue in zebrafish", "case2 6", "case2 6", "Qualified total RNA was purified by DNase I  and mRNA was enriched by adding OligodT  attached magnetic beads. Subsequently  fragment buffer was added to cleave the mRNA into small   fragments. Fragmented mRNA was used as a template for reverse transcription to generate the first  cDNA strand  and then the second cDNA strand was generated. End repair and adaptor ligation were  performed by adding A tailing Mix and RNA Index Adapters  and then PCR amplification was performed. Single strand circle DNA library was obtained by recovering and cycling the PCR products.  The final library was obtained by purifying the linear DNA molecules that were not cycled. The quality  of the final library was tested. The qualified library was replicated by rolling circle replication to form  DNA nanospheres DNBs. DNBs were added to the mesh holes of a nanochip using high density DNA nanochip technology. Subsequently  sequencing was performed on the BGIISEQ500 platform  BGI Shenzhen  Guangdong  China using combined probe anchored polymerisation.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PCR", "PAIRED", "BGISEQ", "BGISEQ-500", null, "SRP429947", null, null, "case2_6_1.fq.gz case2_6_2.fq.gz", "fastq fastq", 6743372700.0, 22477909.0, "case2 6 1.fq.gz", "0:150 1:150", "A:1825699499;C:1533637372;G:1552424547;T:1831611282;N:0", 150, 150, null, null, 1825699499, 1533637372, 1552424547, 1831611282, 0, "SRX19807133", "SRS17168877", "SRA1612461", "Sun Yat-sen University|Department of Epidemiology, School of Public Healt", "Sun Yat-sen University", 2, 0.95425, 0.94313, 0.05313, 0.05246, 0.77268, 0.77461, 0.52616, 0.5227, 150, 150, "B", "B", "biological fallback assumption", "bgi", "bgi", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2023-03-29", "Adult", "Adult", "Bone or Cartilage", "Skeletal Element"], [74711, "SRR24003736", "SRX19807132", "SRS17168877", "SRP429947", "PRJNA949785", "Sequencing of zebrafish bone tissues", "PRJNA949785", "Other", "Transcriptomics was used to investigate sensitive genes associated with exercise improved bone mass", null, null, null, null, "eight month zebrafish bone tissues", null, "breed:Not applicable|age:8 month|sex:male|tissue:b1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of b1 tissue in zebrafish", "case2 5", "case2 5", "Qualified total RNA was purified by DNase I  and mRNA was enriched by adding OligodT  attached magnetic beads. Subsequently  fragment buffer was added to cleave the mRNA into small   fragments. Fragmented mRNA was used as a template for reverse transcription to generate the first  cDNA strand  and then the second cDNA strand was generated. End repair and adaptor ligation were  performed by adding A tailing Mix and RNA Index Adapters  and then PCR amplification was performed. Single strand circle DNA library was obtained by recovering and cycling the PCR products.  The final library was obtained by purifying the linear DNA molecules that were not cycled. The quality  of the final library was tested. The qualified library was replicated by rolling circle replication to form  DNA nanospheres DNBs. DNBs were added to the mesh holes of a nanochip using high density DNA nanochip technology. Subsequently  sequencing was performed on the BGIISEQ500 platform  BGI Shenzhen  Guangdong  China using combined probe anchored polymerisation.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PCR", "PAIRED", "BGISEQ", "BGISEQ-500", null, "SRP429947", null, null, "case2_5_1.fq.gz case2_5_2.fq.gz", "fastq fastq", 6810815400.0, 22702718.0, "case2 5 1.fq.gz", "0:150 1:150", "A:1861549181;C:1529649127;G:1553365612;T:1866251480;N:0", 150, 150, null, null, 1861549181, 1529649127, 1553365612, 1866251480, 0, "SRX19807132", "SRS17168877", "SRA1612461", "Sun Yat-sen University|Department of Epidemiology, School of Public Healt", "Sun Yat-sen University", 2, 0.94842, 0.93757, 0.06448, 0.06317, 0.74036, 0.74097, 0.53479, 0.52393, 150, 150, "B", "B", "biological fallback assumption", "bgi", "bgi", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2023-03-29", "Adult", "Adult", "Bone or Cartilage", "Skeletal Element"], [74712, "SRR24003737", "SRX19807131", "SRS17168877", "SRP429947", "PRJNA949785", "Sequencing of zebrafish bone tissues", "PRJNA949785", "Other", "Transcriptomics was used to investigate sensitive genes associated with exercise improved bone mass", null, null, null, null, "eight month zebrafish bone tissues", null, "breed:Not applicable|age:8 month|sex:male|tissue:b1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of b1 tissue in zebrafish", "control2 2", "control2 2", "Qualified total RNA was purified by DNase I  and mRNA was enriched by adding OligodT  attached magnetic beads. Subsequently  fragment buffer was added to cleave the mRNA into small   fragments. Fragmented mRNA was used as a template for reverse transcription to generate the first  cDNA strand  and then the second cDNA strand was generated. End repair and adaptor ligation were  performed by adding A tailing Mix and RNA Index Adapters  and then PCR amplification was performed. Single strand circle DNA library was obtained by recovering and cycling the PCR products.  The final library was obtained by purifying the linear DNA molecules that were not cycled. The quality  of the final library was tested. The qualified library was replicated by rolling circle replication to form  DNA nanospheres DNBs. DNBs were added to the mesh holes of a nanochip using high density DNA nanochip technology. Subsequently  sequencing was performed on the BGIISEQ500 platform  BGI Shenzhen  Guangdong  China using combined probe anchored polymerisation.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PCR", "PAIRED", "BGISEQ", "BGISEQ-500", null, "SRP429947", null, null, "control2_2_1.fq.gz control2_2_2.fq.gz", "fastq fastq", 6693643800.0, 22312146.0, "control2 2 1.fq.gz", "0:150 1:150", "A:1846873501;C:1499207913;G:1490122627;T:1857439759;N:0", 150, 150, null, null, 1846873501, 1499207913, 1490122627, 1857439759, 0, "SRX19807131", "SRS17168877", "SRA1612461", "Sun Yat-sen University|Department of Epidemiology, School of Public Healt", "Sun Yat-sen University", 2, 0.93282, 0.93092, 0.09007, 0.0899, 0.68672, 0.68793, 0.55221, 0.55318, 150, 150, "B", "B", "biological fallback assumption", "bgi", "bgi", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2023-03-29", "Adult", "Adult", "Bone or Cartilage", "Skeletal Element"], [74713, "SRR24003738", "SRX19807130", "SRS17168877", "SRP429947", "PRJNA949785", "Sequencing of zebrafish bone tissues", "PRJNA949785", "Other", "Transcriptomics was used to investigate sensitive genes associated with exercise improved bone mass", null, null, null, null, "eight month zebrafish bone tissues", null, "breed:Not applicable|age:8 month|sex:male|tissue:b1|BioSampleModel:Model organism or animal", null, null, null, null, null, null, null, null, "RNA Seq of b1 tissue in zebrafish", "control2 1", "control2 1", "Qualified total RNA was purified by DNase I  and mRNA was enriched by adding OligodT  attached magnetic beads. Subsequently  fragment buffer was added to cleave the mRNA into small   fragments. Fragmented mRNA was used as a template for reverse transcription to generate the first  cDNA strand  and then the second cDNA strand was generated. End repair and adaptor ligation were  performed by adding A tailing Mix and RNA Index Adapters  and then PCR amplification was performed. Single strand circle DNA library was obtained by recovering and cycling the PCR products.  The final library was obtained by purifying the linear DNA molecules that were not cycled. The quality  of the final library was tested. The qualified library was replicated by rolling circle replication to form  DNA nanospheres DNBs. DNBs were added to the mesh holes of a nanochip using high density DNA nanochip technology. Subsequently  sequencing was performed on the BGIISEQ500 platform  BGI Shenzhen  Guangdong  China using combined probe anchored polymerisation.", null, null, "RNA-Seq", "TRANSCRIPTOMIC", "PCR", "PAIRED", "BGISEQ", "BGISEQ-500", null, "SRP429947", null, null, "control2_1_1.fq.gz control2_1_2.fq.gz", "fastq fastq", 6629354100.0, 22097847.0, "control2 1 1.fq.gz", "0:150 1:150", "A:1819412655;C:1492313439;G:1486507818;T:1831120188;N:0", 150, 150, null, null, 1819412655, 1492313439, 1486507818, 1831120188, 0, "SRX19807130", "SRS17168877", "SRA1612461", "Sun Yat-sen University|Department of Epidemiology, School of Public Healt", "Sun Yat-sen University", 2, 0.93273, 0.93086, 0.08814, 0.08711, 0.67748, 0.67696, 0.5355, 0.54253, 150, 150, "B", "B", "biological fallback assumption", "bgi", "bgi", "unknown", "random_priming", "unknown", "bulk", "unknown", "unknown", null, "China", "2023-03-29", "Adult", "Adult", "Bone or Cartilage", "Skeletal Element"]], "truncated": false, 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